MS008417 (gene) Bitter gourd (TR) v1

Overview
NameMS008417
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
Descriptionglycosyltransferase family protein 64 C3
Locationscaffold4: 1258594 .. 1259556 (+)
RNA-Seq ExpressionMS008417
SyntenyMS008417
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAATTCAAGTCACCGACGATCTTCTTCTTCCTTCTCTCTTTTTCCGCCGCCGTGTCCTCAACCGCCGACCGGTGCGCCGCCGAATTCCGGCCGGACCGACGGAATTTAAGATCCGATCAAATCACGGTCCTCATCAACGGCTATTACGAGTCCCGCATCCCCCTCCTCCAATCAATAGCCTCCACGTACGCCGCATCCCCATTCGTACGCGCCGTGCTCGTTCTTTGGGGAAATCCCTCAACCCCAAGAGAAACCCTAACTCAATTGGCTCGAAACCTCACCGCCGGACCAATTTCCCTAATTCGCCAATCTTCAAACAGTCTCAACGCTCGATTTCTCCCCCGCAGGTCCATCGAAACCGGCGTCGTTTTGATCTGCGACGACGATGTCGAAATCGACACGAAATCGCTCGAATTCGCGTTCAGAATTTGGGGGAGAAACCCCGAACGACTTGTCGGCTTCTTCGTCCGGTCGCACGATATAGACCTCTCGAAGAGGGAATGGATCTACACCGTTCATCCAGACAAGTACTCGATCGTGCTCACCAAGCTCATGATCCTGAAAACAGAGTATCTGTTCGAGTACAGCTGCGGCGGCGGAGAGGCGATGGCCGACATGAGGAGAGTTGTCGATCGGGCGCGTAATTGCGAGGATATCTTGATGAATTTCGTGGTGGCCGACATGTCGAACGCCGGGCCTATACTGGTTGCGGCGCAGAGGATCAGGGATTGGGGGGACCCACGGAATGATGATGGCGACGAACGGCTGGGATTGACTGAGGTGGGGCTGAGTAATAGGAAAGGGCAGCATAGGAAGAGAAGGGGACGGTGCATAACGGAGTTTCATAGACTGTTGGGGAGGATGCCGTTACGGTATAGTTACGGAAAGTTCGTTAATTCAGTTGGCGAGCAAGGTCTATGTAGAAAAGGAGGGAAATTGGTCCCCTGTGATCAAAATATC

mRNA sequence

ATGAAATTCAAGTCACCGACGATCTTCTTCTTCCTTCTCTCTTTTTCCGCCGCCGTGTCCTCAACCGCCGACCGGTGCGCCGCCGAATTCCGGCCGGACCGACGGAATTTAAGATCCGATCAAATCACGGTCCTCATCAACGGCTATTACGAGTCCCGCATCCCCCTCCTCCAATCAATAGCCTCCACGTACGCCGCATCCCCATTCGTACGCGCCGTGCTCGTTCTTTGGGGAAATCCCTCAACCCCAAGAGAAACCCTAACTCAATTGGCTCGAAACCTCACCGCCGGACCAATTTCCCTAATTCGCCAATCTTCAAACAGTCTCAACGCTCGATTTCTCCCCCGCAGGTCCATCGAAACCGGCGTCGTTTTGATCTGCGACGACGATGTCGAAATCGACACGAAATCGCTCGAATTCGCGTTCAGAATTTGGGGGAGAAACCCCGAACGACTTGTCGGCTTCTTCGTCCGGTCGCACGATATAGACCTCTCGAAGAGGGAATGGATCTACACCGTTCATCCAGACAAGTACTCGATCGTGCTCACCAAGCTCATGATCCTGAAAACAGAGTATCTGTTCGAGTACAGCTGCGGCGGCGGAGAGGCGATGGCCGACATGAGGAGAGTTGTCGATCGGGCGCGTAATTGCGAGGATATCTTGATGAATTTCGTGGTGGCCGACATGTCGAACGCCGGGCCTATACTGGTTGCGGCGCAGAGGATCAGGGATTGGGGGGACCCACGGAATGATGATGGCGACGAACGGCTGGGATTGACTGAGGTGGGGCTGAGTAATAGGAAAGGGCAGCATAGGAAGAGAAGGGGACGGTGCATAACGGAGTTTCATAGACTGTTGGGGAGGATGCCGTTACGGTATAGTTACGGAAAGTTCGTTAATTCAGTTGGCGAGCAAGGTCTATGTAGAAAAGGAGGGAAATTGGTCCCCTGTGATCAAAATATC

Coding sequence (CDS)

ATGAAATTCAAGTCACCGACGATCTTCTTCTTCCTTCTCTCTTTTTCCGCCGCCGTGTCCTCAACCGCCGACCGGTGCGCCGCCGAATTCCGGCCGGACCGACGGAATTTAAGATCCGATCAAATCACGGTCCTCATCAACGGCTATTACGAGTCCCGCATCCCCCTCCTCCAATCAATAGCCTCCACGTACGCCGCATCCCCATTCGTACGCGCCGTGCTCGTTCTTTGGGGAAATCCCTCAACCCCAAGAGAAACCCTAACTCAATTGGCTCGAAACCTCACCGCCGGACCAATTTCCCTAATTCGCCAATCTTCAAACAGTCTCAACGCTCGATTTCTCCCCCGCAGGTCCATCGAAACCGGCGTCGTTTTGATCTGCGACGACGATGTCGAAATCGACACGAAATCGCTCGAATTCGCGTTCAGAATTTGGGGGAGAAACCCCGAACGACTTGTCGGCTTCTTCGTCCGGTCGCACGATATAGACCTCTCGAAGAGGGAATGGATCTACACCGTTCATCCAGACAAGTACTCGATCGTGCTCACCAAGCTCATGATCCTGAAAACAGAGTATCTGTTCGAGTACAGCTGCGGCGGCGGAGAGGCGATGGCCGACATGAGGAGAGTTGTCGATCGGGCGCGTAATTGCGAGGATATCTTGATGAATTTCGTGGTGGCCGACATGTCGAACGCCGGGCCTATACTGGTTGCGGCGCAGAGGATCAGGGATTGGGGGGACCCACGGAATGATGATGGCGACGAACGGCTGGGATTGACTGAGGTGGGGCTGAGTAATAGGAAAGGGCAGCATAGGAAGAGAAGGGGACGGTGCATAACGGAGTTTCATAGACTGTTGGGGAGGATGCCGTTACGGTATAGTTACGGAAAGTTCGTTAATTCAGTTGGCGAGCAAGGTCTATGTAGAAAAGGAGGGAAATTGGTCCCCTGTGATCAAAATATC

Protein sequence

MKFKSPTIFFFLLSFSAAVSSTADRCAAEFRPDRRNLRSDQITVLINGYYESRIPLLQSIASTYAASPFVRAVLVLWGNPSTPRETLTQLARNLTAGPISLIRQSSNSLNARFLPRRSIETGVVLICDDDVEIDTKSLEFAFRIWGRNPERLVGFFVRSHDIDLSKREWIYTVHPDKYSIVLTKLMILKTEYLFEYSCGGGEAMADMRRVVDRARNCEDILMNFVVADMSNAGPILVAAQRIRDWGDPRNDDGDERLGLTEVGLSNRKGQHRKRRGRCITEFHRLLGRMPLRYSYGKFVNSVGEQGLCRKGGKLVPCDQNI
Homology
BLAST of MS008417 vs. NCBI nr
Match: XP_022137713.1 (glycosyltransferase family protein 64 C3 [Momordica charantia])

HSP 1 Score: 645.2 bits (1663), Expect = 3.0e-181
Identity = 319/321 (99.38%), Postives = 319/321 (99.38%), Query Frame = 0

Query: 1   MKFKSPTIFFFLLSFSAAVSSTADRCAAEFRPDRRNLRSDQITVLINGYYESRIPLLQSI 60
           MKFKSPTIFFFLLSFSAAVSSTADRCAAEFRPDRRNLRSDQITVLINGYYESRIPLLQSI
Sbjct: 1   MKFKSPTIFFFLLSFSAAVSSTADRCAAEFRPDRRNLRSDQITVLINGYYESRIPLLQSI 60

Query: 61  ASTYAASPFVRAVLVLWGNPSTPRETLTQLARNLTAGPISLIRQSSNSLNARFLPRRSIE 120
           ASTYAASPFVRAVLVLWGNPSTPRETLTQLARNLTAGPISLIRQSSNSLNARFLPRRSIE
Sbjct: 61  ASTYAASPFVRAVLVLWGNPSTPRETLTQLARNLTAGPISLIRQSSNSLNARFLPRRSIE 120

Query: 121 TGVVLICDDDVEIDTKSLEFAFRIWGRNPERLVGFFVRSHDIDLSKREWIYTVHPDKYSI 180
           TG VLICDDDVEIDTKSLEFAFRIWGRNPERLVGFFVRSHDIDLSKREWIYTVHPDKYSI
Sbjct: 121 TGAVLICDDDVEIDTKSLEFAFRIWGRNPERLVGFFVRSHDIDLSKREWIYTVHPDKYSI 180

Query: 181 VLTKLMILKTEYLFEYSCGGGEAMADMRRVVDRARNCEDILMNFVVADMSNAGPILVAAQ 240
           VLTKLMILKTEYLFEYSCGGGEAMADMRRVVDRARNCEDILMNFVVADMSNAGPILVAAQ
Sbjct: 181 VLTKLMILKTEYLFEYSCGGGEAMADMRRVVDRARNCEDILMNFVVADMSNAGPILVAAQ 240

Query: 241 RIRDWGDPRNDDGDERLGLTEVGLSNRKGQHRKRRGRCITEFHRLLGRMPLRYSYGKFVN 300
           RIRDWGDPRNDDG ERLGLTEVGLSNRKGQHRKRRGRCITEFHRLLGRMPLRYSYGKFVN
Sbjct: 241 RIRDWGDPRNDDGHERLGLTEVGLSNRKGQHRKRRGRCITEFHRLLGRMPLRYSYGKFVN 300

Query: 301 SVGEQGLCRKGGKLVPCDQNI 322
           SVGEQGLCRKGGKLVPCDQNI
Sbjct: 301 SVGEQGLCRKGGKLVPCDQNI 321

BLAST of MS008417 vs. NCBI nr
Match: XP_038898918.1 (glycosyltransferase family protein 64 C3 [Benincasa hispida])

HSP 1 Score: 543.9 bits (1400), Expect = 9.4e-151
Identity = 275/331 (83.08%), Postives = 292/331 (88.22%), Query Frame = 0

Query: 1   MKFKSPTIFFFLLSFSAAVSS----TADRCAAEFRPDRRNLRSDQITVLINGYYESRIPL 60
           M F   TI   LLSFS AVSS    TA  CAA   PDRRNLRSDQITVLINGYYESRIPL
Sbjct: 1   MNFHFLTILVLLLSFSTAVSSLHEETAATCAAASPPDRRNLRSDQITVLINGYYESRIPL 60

Query: 61  LQSIASTYAASPFVRAVLVLWGNPSTPRETLTQLARNLTAGPISLIRQSSNSLNARFLPR 120
           LQSIASTYAASPFV  VL+LWGNPST  +TLT+LA+NLT GPISLIRQSSNSLN+RFLPR
Sbjct: 61  LQSIASTYAASPFVHTVLILWGNPSTSTKTLTKLAQNLTTGPISLIRQSSNSLNSRFLPR 120

Query: 121 RSIETGVVLICDDDVEIDTKSLEFAFRIWGRNPERLVGFFVRSHDIDLSKREWIYTVHPD 180
           +SI+TG VLICDDDVEIDT SLEFAFRIWGRNPERLVGFFVRSHD+DLS+REWIYTVH D
Sbjct: 121 KSIQTGAVLICDDDVEIDTSSLEFAFRIWGRNPERLVGFFVRSHDLDLSRREWIYTVHQD 180

Query: 181 KYSIVLTKLMILKTEYLFEYSCGGGEAMADMRRVVDRARNCEDILMNFVVADMSNAGPIL 240
           +YSIVLTKLMILKTEYLFEYSCGGG AMADMRRVVDR RNCEDILMNFVVADMSNAGPI+
Sbjct: 181 RYSIVLTKLMILKTEYLFEYSCGGGAAMADMRRVVDRERNCEDILMNFVVADMSNAGPIM 240

Query: 241 VAAQRIRDWGDPRN--DDGDERLGL----TEVGLSNRKGQHRKRRGRCITEFHRLLGRMP 300
           VAAQRIRDWGDPRN  DD +ERLGL    +E+GLSNRKG+HRKRRGRCITEFHR  GRMP
Sbjct: 241 VAAQRIRDWGDPRNDYDDENERLGLREGVSEIGLSNRKGEHRKRRGRCITEFHRRFGRMP 300

Query: 301 LRYSYGKFVNSVGEQGLCRKGGKLVPCDQNI 322
           LRYSYGK V+SVGEQ LCRKG KLVPCDQN+
Sbjct: 301 LRYSYGKSVDSVGEQALCRKGRKLVPCDQNV 331

BLAST of MS008417 vs. NCBI nr
Match: KAG7024528.1 (Glycosyltransferase family protein 64 C3, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 543.9 bits (1400), Expect = 9.4e-151
Identity = 275/330 (83.33%), Postives = 294/330 (89.09%), Query Frame = 0

Query: 1   MKFKSPTIFFFLLSFSAAVSS----TADRCAAEFRPDRRNLRSDQITVLINGYYESRIPL 60
           M FKS TI F LLSFS AV S    TA  C AE +PDRRNLRSDQITVLINGYYESRIPL
Sbjct: 1   MNFKSLTI-FILLSFSTAVCSLLKATAAACDAETQPDRRNLRSDQITVLINGYYESRIPL 60

Query: 61  LQSIASTYAASPFVRAVLVLWGNPSTPRETLTQLARNLTAGPISLIRQSSNSLNARFLPR 120
           LQSIA+TYAASP V+AVL+LWGNPST  +TLTQLA+NLT GPIS+IRQSSNSLN+RFLP 
Sbjct: 61  LQSIAATYAASPIVQAVLILWGNPSTSSKTLTQLAQNLTTGPISVIRQSSNSLNSRFLPH 120

Query: 121 RSIETGVVLICDDDVEIDTKSLEFAFRIWGRNPERLVGFFVRSHDIDLSKREWIYTVHPD 180
           +SI+TG VLICDDDVEID  SLEFAFR+WGRNPERLVGFFVRSHD+DLS+REWIYTVH D
Sbjct: 121 KSIQTGAVLICDDDVEIDISSLEFAFRVWGRNPERLVGFFVRSHDLDLSRREWIYTVHQD 180

Query: 181 KYSIVLTKLMILKTEYLFEYSCGGGEAMADMRRVVDRARNCEDILMNFVVADMSNAGPIL 240
           KYSIVLTKLMILK EYLFEY+CGGG AMADMRRVVDR RNCEDILMNF+VADMSNAGPIL
Sbjct: 181 KYSIVLTKLMILKMEYLFEYTCGGGAAMADMRRVVDRERNCEDILMNFMVADMSNAGPIL 240

Query: 241 VAAQRIRDWGDPRND-DGDERLGL----TEVGLSNRKGQHRKRRGRCITEFHRLLGRMPL 300
           VAAQRIRDWGDPRND D +ERLGL    +EVGLSNRKG+HRKRRGRCITEFHR LGRMPL
Sbjct: 241 VAAQRIRDWGDPRNDYDENERLGLGEGVSEVGLSNRKGEHRKRRGRCITEFHRRLGRMPL 300

Query: 301 RYSYGKFVNSVGEQGLCRKGGKLVPCDQNI 322
           RYSYGK VNSVGEQ LCRKGGKLVPCD N+
Sbjct: 301 RYSYGKLVNSVGEQALCRKGGKLVPCDHNV 329

BLAST of MS008417 vs. NCBI nr
Match: KAG6591645.1 (Glycosyltransferase family protein 64 C3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 543.9 bits (1400), Expect = 9.4e-151
Identity = 275/330 (83.33%), Postives = 294/330 (89.09%), Query Frame = 0

Query: 1   MKFKSPTIFFFLLSFSAAVSS----TADRCAAEFRPDRRNLRSDQITVLINGYYESRIPL 60
           M FKS TI F LLSFS AV S    TA  C AE +PDRRNLRSDQITVLINGYYESRIPL
Sbjct: 1   MNFKSLTI-FILLSFSTAVCSLLKATAAACDAESQPDRRNLRSDQITVLINGYYESRIPL 60

Query: 61  LQSIASTYAASPFVRAVLVLWGNPSTPRETLTQLARNLTAGPISLIRQSSNSLNARFLPR 120
           LQSIA+TYAASP V+AVL+LWGNPST  +TLTQLA+NLT GPIS+IRQSSNSLN+RFLP 
Sbjct: 61  LQSIAATYAASPIVQAVLILWGNPSTSSKTLTQLAQNLTTGPISVIRQSSNSLNSRFLPH 120

Query: 121 RSIETGVVLICDDDVEIDTKSLEFAFRIWGRNPERLVGFFVRSHDIDLSKREWIYTVHPD 180
           +SI+TG VLICDDDVEID  SLEFAFR+WGRNPERLVGFFVRSHD+DLS+REWIYTVH D
Sbjct: 121 KSIQTGAVLICDDDVEIDISSLEFAFRVWGRNPERLVGFFVRSHDLDLSRREWIYTVHQD 180

Query: 181 KYSIVLTKLMILKTEYLFEYSCGGGEAMADMRRVVDRARNCEDILMNFVVADMSNAGPIL 240
           KYSIVLTKLMILK EYLFEY+CGGG AMADMRRVVDR RNCEDILMNF+VADMSNAGPIL
Sbjct: 181 KYSIVLTKLMILKMEYLFEYTCGGGAAMADMRRVVDRERNCEDILMNFMVADMSNAGPIL 240

Query: 241 VAAQRIRDWGDPRND-DGDERLGL----TEVGLSNRKGQHRKRRGRCITEFHRLLGRMPL 300
           VAAQRIRDWGDPRND D +ERLGL    +EVGLSNRKG+HRKRRGRCITEFHR LGRMPL
Sbjct: 241 VAAQRIRDWGDPRNDYDENERLGLGEGVSEVGLSNRKGEHRKRRGRCITEFHRRLGRMPL 300

Query: 301 RYSYGKFVNSVGEQGLCRKGGKLVPCDQNI 322
           RYSYGK VNSVGEQ LCRKGGKLVPCD N+
Sbjct: 301 RYSYGKLVNSVGEQALCRKGGKLVPCDHNV 329

BLAST of MS008417 vs. NCBI nr
Match: XP_022975936.1 (glycosyltransferase family protein 64 C3 [Cucurbita maxima])

HSP 1 Score: 542.3 bits (1396), Expect = 2.7e-150
Identity = 275/330 (83.33%), Postives = 292/330 (88.48%), Query Frame = 0

Query: 1   MKFKSPTIFFFLLSFSAAVSS----TADRCAAEFRPDRRNLRSDQITVLINGYYESRIPL 60
           M FKS TI F LLSFS AV S    TA  C AE  PDRRNLRSDQITVLINGYYESRIPL
Sbjct: 1   MNFKSLTI-FILLSFSTAVCSLLKATAAACDAESLPDRRNLRSDQITVLINGYYESRIPL 60

Query: 61  LQSIASTYAASPFVRAVLVLWGNPSTPRETLTQLARNLTAGPISLIRQSSNSLNARFLPR 120
           LQSIA+TYAASP V AVL+LWGNPST  +TLTQLA+NLT GPIS+IRQSSNSLN+RFLP 
Sbjct: 61  LQSIAATYAASPIVHAVLILWGNPSTSSKTLTQLAQNLTTGPISVIRQSSNSLNSRFLPH 120

Query: 121 RSIETGVVLICDDDVEIDTKSLEFAFRIWGRNPERLVGFFVRSHDIDLSKREWIYTVHPD 180
           +SI+TG VLICDDDVEID  SLEFAFR+WGRNPERLVGFFVRSHD+DLS+REWIYTVH D
Sbjct: 121 KSIQTGAVLICDDDVEIDISSLEFAFRVWGRNPERLVGFFVRSHDLDLSRREWIYTVHQD 180

Query: 181 KYSIVLTKLMILKTEYLFEYSCGGGEAMADMRRVVDRARNCEDILMNFVVADMSNAGPIL 240
           KYSIVLTKLMILK EYLFEY+CGGG AMADMRRVVDR RNCEDILMNF+VADMSNAGPIL
Sbjct: 181 KYSIVLTKLMILKMEYLFEYTCGGGAAMADMRRVVDRERNCEDILMNFMVADMSNAGPIL 240

Query: 241 VAAQRIRDWGDPRND-DGDERLGL----TEVGLSNRKGQHRKRRGRCITEFHRLLGRMPL 300
           VAAQRIRDWGDPRND D +ERLGL    +EVGLSNRKG+HRKRRGRCITEFHR LGRMPL
Sbjct: 241 VAAQRIRDWGDPRNDYDENERLGLGERFSEVGLSNRKGEHRKRRGRCITEFHRRLGRMPL 300

Query: 301 RYSYGKFVNSVGEQGLCRKGGKLVPCDQNI 322
           RYSYGK VNSVGEQ LCRKGGKLVPCD N+
Sbjct: 301 RYSYGKLVNSVGEQALCRKGGKLVPCDHNV 329

BLAST of MS008417 vs. ExPASy Swiss-Prot
Match: Q9C975 (Glycosyltransferase family protein 64 C3 OS=Arabidopsis thaliana OX=3702 GN=At1g80290 PE=2 SV=1)

HSP 1 Score: 409.1 bits (1050), Expect = 4.7e-113
Identity = 201/313 (64.22%), Postives = 245/313 (78.27%), Query Frame = 0

Query: 13  LSFSAAVSSTADRCAAEFRPDRRNLRSDQITVLINGYYESRIPLLQSIASTYAASPFVRA 72
           L F   +S   D C A  + + + LRSDQITVLINGY E RIPLLQ+I ++Y++S  V +
Sbjct: 19  LVFCRTLSGDPDPCDATNQREFQKLRSDQITVLINGYSEYRIPLLQTIVASYSSSSIVSS 78

Query: 73  VLVLWGNPSTPRETLTQLARNLT-----AGPISLIRQSSNSLNARFLPRRSIETGVVLIC 132
           +LVLWGNPSTP + L QL +NLT     +  ISLI+QSS+SLNARFLPR S++T  VLIC
Sbjct: 79  ILVLWGNPSTPDQLLDQLYQNLTQYSPGSASISLIQQSSSSLNARFLPRSSVDTRAVLIC 138

Query: 133 DDDVEIDTKSLEFAFRIWGRNPERLVGFFVRSHDIDLSKREWIYTVHPDKYSIVLTKLMI 192
           DDDVEID +SLEFAF +W  NP+RLVG FVRSH  DL  +EWIYTVHPDKYSIVLTK M+
Sbjct: 139 DDDVEIDQRSLEFAFSVWKSNPDRLVGTFVRSHGFDLQGKEWIYTVHPDKYSIVLTKFMM 198

Query: 193 LKTEYLFEYSCGGGEAMADMRRVVDRARNCEDILMNFVVADMSNAGPILVAAQRIRDWGD 252
           +K +YLFEYSC GG  M +MR +VD+ RNCEDILMNFV AD   AGPI+V A+R+RDWGD
Sbjct: 199 MKQDYLFEYSCKGGVEMEEMRMIVDQMRNCEDILMNFVAADRLRAGPIMVGAERVRDWGD 258

Query: 253 PRNDDGDERLGLTEVGLSNRKGQHRKRRGRCITEFHRLLGRMPLRYSYGKFVNSVGEQGL 312
            RN++ +ER  + +VGLS+R+ +HRKRRG CI EFHR++G+MPL YSYGK VNSVGEQGL
Sbjct: 259 ARNEEVEER--VRDVGLSSRRVEHRKRRGNCIREFHRVMGKMPLMYSYGKVVNSVGEQGL 318

Query: 313 CRKGGKLVPCDQN 321
           CRK GKLV CD++
Sbjct: 319 CRKAGKLVFCDRD 329

BLAST of MS008417 vs. ExPASy Swiss-Prot
Match: Q9WVL6 (Exostosin-like 3 OS=Mus musculus OX=10090 GN=Extl3 PE=1 SV=2)

HSP 1 Score: 120.6 bits (301), Expect = 3.4e-26
Identity = 84/261 (32.18%), Postives = 127/261 (48.66%), Query Frame = 0

Query: 36  NLRSDQITVLINGYYESRIPLLQSIASTYAASPFVRAVLVLWGNPSTPRETLTQLARNLT 95
           N++ +Q TV++  Y   R  +L +        P++  V+V+W +P  P E L      + 
Sbjct: 656 NVQREQFTVVMLTY--EREEVLMNSLERLNGLPYLNKVVVVWNSPKLPSEDLLWPDIGV- 715

Query: 96  AGPISLIRQSSNSLNARFLPRRSIETGVVLICDDDVEIDTKSLEFAFRIWGRNPERLVGF 155
             PI ++R   NSLN RFLP   IET  +L  DDD  +    + F FR+W    +R+VGF
Sbjct: 716 --PIMVVRTEKNSLNNRFLPWNEIETEAILSIDDDAHLRHDEIMFGFRVWREARDRIVGF 775

Query: 156 FVRSHDIDLSKREWIY-TVHPDKYSIVLTKLMILKTEYLFEYSCGGGEAMADMRRVVDRA 215
             R H  D+  + W+Y + +  + S+VLT        Y + YS    +A+ DM   VD  
Sbjct: 776 PGRYHAWDIPHQSWLYNSNYSCELSMVLTGAAFFHKYYAYLYSYVMPQAIRDM---VDEY 835

Query: 216 RNCEDILMNFVVADMSNAGPILVAAQ-RIRDWGDPRNDDGDERLGLTEVGLSNRKGQHRK 275
            NCEDI MNF+V+ ++   PI V ++   R  G P+    D+               H  
Sbjct: 836 INCEDIAMNFLVSHITRKPPIKVTSRWTFRCPGCPQALSHDD--------------SHFH 894

Query: 276 RRGRCITEFHRLLGRMPLRYS 295
            R +CI  F ++ G MPL Y+
Sbjct: 896 ERHKCINFFVKVYGYMPLLYT 894

BLAST of MS008417 vs. ExPASy Swiss-Prot
Match: O43909 (Exostosin-like 3 OS=Homo sapiens OX=9606 GN=EXTL3 PE=1 SV=1)

HSP 1 Score: 119.4 bits (298), Expect = 7.5e-26
Identity = 84/261 (32.18%), Postives = 126/261 (48.28%), Query Frame = 0

Query: 36  NLRSDQITVLINGYYESRIPLLQSIASTYAASPFVRAVLVLWGNPSTPRETLTQLARNLT 95
           N+  +Q TV++  Y   R  +L +        P++  V+V+W +P  P E L      + 
Sbjct: 657 NVPREQFTVVMLTY--EREEVLMNSLERLNGLPYLNKVVVVWNSPKLPSEDLLWPDIGV- 716

Query: 96  AGPISLIRQSSNSLNARFLPRRSIETGVVLICDDDVEIDTKSLEFAFRIWGRNPERLVGF 155
             PI ++R   NSLN RFLP   IET  +L  DDD  +    + F FR+W    +R+VGF
Sbjct: 717 --PIMVVRTEKNSLNNRFLPWNEIETEAILSIDDDAHLRHDEIMFGFRVWREARDRIVGF 776

Query: 156 FVRSHDIDLSKREWIY-TVHPDKYSIVLTKLMILKTEYLFEYSCGGGEAMADMRRVVDRA 215
             R H  D+  + W+Y + +  + S+VLT        Y + YS    +A+ DM   VD  
Sbjct: 777 PGRYHAWDIPHQSWLYNSNYSCELSMVLTGAAFFHKYYAYLYSYVMPQAIRDM---VDEY 836

Query: 216 RNCEDILMNFVVADMSNAGPILVAAQ-RIRDWGDPRNDDGDERLGLTEVGLSNRKGQHRK 275
            NCEDI MNF+V+ ++   PI V ++   R  G P+    D+               H  
Sbjct: 837 INCEDIAMNFLVSHITRKPPIKVTSRWTFRCPGCPQALSHDD--------------SHFH 895

Query: 276 RRGRCITEFHRLLGRMPLRYS 295
            R +CI  F ++ G MPL Y+
Sbjct: 897 ERHKCINFFVKVYGYMPLLYT 895

BLAST of MS008417 vs. ExPASy Swiss-Prot
Match: Q5RBC3 (Exostosin-1 OS=Pongo abelii OX=9601 GN=EXT1 PE=2 SV=1)

HSP 1 Score: 110.5 bits (275), Expect = 3.5e-23
Identity = 81/246 (32.93%), Postives = 122/246 (49.59%), Query Frame = 0

Query: 55  PLLQSIASTYAASPFVRAVLVLWGNPSTPRETLTQLARNLTAGPISLIRQSSNSLNARFL 114
           P+L+ + +  A S +   ++VLW N   P     +     TA P+ +I   S  +++RFL
Sbjct: 496 PVLKLLVAA-AKSQYCAQIIVLW-NCDKPLPAKHRWP--ATAVPVIVIEGESKVMSSRFL 555

Query: 115 PRRSIETGVVLICDDDVEIDTKSLEFAFRIWGRNPERLVGFFVRSHDIDLSKREWIYTVH 174
           P  +I T  VL  D+D  + T  ++FAF +W   PER+VG+  RSH  D SK  W YT  
Sbjct: 556 PYDNIITDAVLSLDEDTVLSTTEVDFAFTVWRSFPERIVGYPARSHFWDNSKERWGYTSK 615

Query: 175 -PDKYSIVLTKLMILKTEYLFEYSCGGGEAMADMRRVVDRARNCEDILMNFVVADMSNAG 234
             + YS+VLT   I    Y + YS       A ++ +VD+  NCEDILMNF+V+ ++   
Sbjct: 616 WTNDYSMVLTGAAIYHKYYHYLYS---HYLPASLKNMVDQLANCEDILMNFLVSAVTKLP 675

Query: 235 PILVAAQRIRDWGDPRNDDGDERLGLTEVGLSNRKGQ-----HRKRRGRCITEFHRLLGR 294
           PI V  +              E+   T +G ++R  +     H  +R  C+  F    G 
Sbjct: 676 PIKVTQK--------------EQYKETMMGQTSRASRWADPDHFAQRQSCMNTFASWFGY 720

BLAST of MS008417 vs. ExPASy Swiss-Prot
Match: Q16394 (Exostosin-1 OS=Homo sapiens OX=9606 GN=EXT1 PE=1 SV=2)

HSP 1 Score: 110.2 bits (274), Expect = 4.5e-23
Identity = 79/241 (32.78%), Postives = 118/241 (48.96%), Query Frame = 0

Query: 55  PLLQSIASTYAASPFVRAVLVLWGNPSTPRETLTQLARNLTAGPISLIRQSSNSLNARFL 114
           P+L+ + +  A S +   ++VLW N   P     +     TA P+ +I   S  +++RFL
Sbjct: 496 PVLKLLVAA-AKSQYCAQIIVLW-NCDKPLPAKHRWP--ATAVPVVVIEGESKVMSSRFL 555

Query: 115 PRRSIETGVVLICDDDVEIDTKSLEFAFRIWGRNPERLVGFFVRSHDIDLSKREWIYTVH 174
           P  +I T  VL  D+D  + T  ++FAF +W   PER+VG+  RSH  D SK  W YT  
Sbjct: 556 PYDNIITDAVLSLDEDTVLSTTEVDFAFTVWQSFPERIVGYPARSHFWDNSKERWGYTSK 615

Query: 175 -PDKYSIVLTKLMILKTEYLFEYSCGGGEAMADMRRVVDRARNCEDILMNFVVADMSNAG 234
             + YS+VLT   I    Y + YS       A ++ +VD+  NCEDILMNF+V+ ++   
Sbjct: 616 WTNDYSMVLTGAAIYHKYYHYLYS---HYLPASLKNMVDQLANCEDILMNFLVSAVTKLP 675

Query: 235 PILVAAQRIRDWGDPRNDDGDERLGLTEVGLSNRKGQHRKRRGRCITEFHRLLGRMPLRY 294
           PI V  ++            +  +G T          H  +R  C+  F    G MPL +
Sbjct: 676 PIKVTQKK---------QYKETMMGQTSRASRWADPDHFAQRQSCMNTFASWFGYMPLIH 720

BLAST of MS008417 vs. ExPASy TrEMBL
Match: A0A6J1CB50 (glycosyltransferase family protein 64 C3 OS=Momordica charantia OX=3673 GN=LOC111009080 PE=3 SV=1)

HSP 1 Score: 645.2 bits (1663), Expect = 1.4e-181
Identity = 319/321 (99.38%), Postives = 319/321 (99.38%), Query Frame = 0

Query: 1   MKFKSPTIFFFLLSFSAAVSSTADRCAAEFRPDRRNLRSDQITVLINGYYESRIPLLQSI 60
           MKFKSPTIFFFLLSFSAAVSSTADRCAAEFRPDRRNLRSDQITVLINGYYESRIPLLQSI
Sbjct: 1   MKFKSPTIFFFLLSFSAAVSSTADRCAAEFRPDRRNLRSDQITVLINGYYESRIPLLQSI 60

Query: 61  ASTYAASPFVRAVLVLWGNPSTPRETLTQLARNLTAGPISLIRQSSNSLNARFLPRRSIE 120
           ASTYAASPFVRAVLVLWGNPSTPRETLTQLARNLTAGPISLIRQSSNSLNARFLPRRSIE
Sbjct: 61  ASTYAASPFVRAVLVLWGNPSTPRETLTQLARNLTAGPISLIRQSSNSLNARFLPRRSIE 120

Query: 121 TGVVLICDDDVEIDTKSLEFAFRIWGRNPERLVGFFVRSHDIDLSKREWIYTVHPDKYSI 180
           TG VLICDDDVEIDTKSLEFAFRIWGRNPERLVGFFVRSHDIDLSKREWIYTVHPDKYSI
Sbjct: 121 TGAVLICDDDVEIDTKSLEFAFRIWGRNPERLVGFFVRSHDIDLSKREWIYTVHPDKYSI 180

Query: 181 VLTKLMILKTEYLFEYSCGGGEAMADMRRVVDRARNCEDILMNFVVADMSNAGPILVAAQ 240
           VLTKLMILKTEYLFEYSCGGGEAMADMRRVVDRARNCEDILMNFVVADMSNAGPILVAAQ
Sbjct: 181 VLTKLMILKTEYLFEYSCGGGEAMADMRRVVDRARNCEDILMNFVVADMSNAGPILVAAQ 240

Query: 241 RIRDWGDPRNDDGDERLGLTEVGLSNRKGQHRKRRGRCITEFHRLLGRMPLRYSYGKFVN 300
           RIRDWGDPRNDDG ERLGLTEVGLSNRKGQHRKRRGRCITEFHRLLGRMPLRYSYGKFVN
Sbjct: 241 RIRDWGDPRNDDGHERLGLTEVGLSNRKGQHRKRRGRCITEFHRLLGRMPLRYSYGKFVN 300

Query: 301 SVGEQGLCRKGGKLVPCDQNI 322
           SVGEQGLCRKGGKLVPCDQNI
Sbjct: 301 SVGEQGLCRKGGKLVPCDQNI 321

BLAST of MS008417 vs. ExPASy TrEMBL
Match: A0A6J1IFJ6 (glycosyltransferase family protein 64 C3 OS=Cucurbita maxima OX=3661 GN=LOC111476492 PE=3 SV=1)

HSP 1 Score: 542.3 bits (1396), Expect = 1.3e-150
Identity = 275/330 (83.33%), Postives = 292/330 (88.48%), Query Frame = 0

Query: 1   MKFKSPTIFFFLLSFSAAVSS----TADRCAAEFRPDRRNLRSDQITVLINGYYESRIPL 60
           M FKS TI F LLSFS AV S    TA  C AE  PDRRNLRSDQITVLINGYYESRIPL
Sbjct: 1   MNFKSLTI-FILLSFSTAVCSLLKATAAACDAESLPDRRNLRSDQITVLINGYYESRIPL 60

Query: 61  LQSIASTYAASPFVRAVLVLWGNPSTPRETLTQLARNLTAGPISLIRQSSNSLNARFLPR 120
           LQSIA+TYAASP V AVL+LWGNPST  +TLTQLA+NLT GPIS+IRQSSNSLN+RFLP 
Sbjct: 61  LQSIAATYAASPIVHAVLILWGNPSTSSKTLTQLAQNLTTGPISVIRQSSNSLNSRFLPH 120

Query: 121 RSIETGVVLICDDDVEIDTKSLEFAFRIWGRNPERLVGFFVRSHDIDLSKREWIYTVHPD 180
           +SI+TG VLICDDDVEID  SLEFAFR+WGRNPERLVGFFVRSHD+DLS+REWIYTVH D
Sbjct: 121 KSIQTGAVLICDDDVEIDISSLEFAFRVWGRNPERLVGFFVRSHDLDLSRREWIYTVHQD 180

Query: 181 KYSIVLTKLMILKTEYLFEYSCGGGEAMADMRRVVDRARNCEDILMNFVVADMSNAGPIL 240
           KYSIVLTKLMILK EYLFEY+CGGG AMADMRRVVDR RNCEDILMNF+VADMSNAGPIL
Sbjct: 181 KYSIVLTKLMILKMEYLFEYTCGGGAAMADMRRVVDRERNCEDILMNFMVADMSNAGPIL 240

Query: 241 VAAQRIRDWGDPRND-DGDERLGL----TEVGLSNRKGQHRKRRGRCITEFHRLLGRMPL 300
           VAAQRIRDWGDPRND D +ERLGL    +EVGLSNRKG+HRKRRGRCITEFHR LGRMPL
Sbjct: 241 VAAQRIRDWGDPRNDYDENERLGLGERFSEVGLSNRKGEHRKRRGRCITEFHRRLGRMPL 300

Query: 301 RYSYGKFVNSVGEQGLCRKGGKLVPCDQNI 322
           RYSYGK VNSVGEQ LCRKGGKLVPCD N+
Sbjct: 301 RYSYGKLVNSVGEQALCRKGGKLVPCDHNV 329

BLAST of MS008417 vs. ExPASy TrEMBL
Match: A0A5D3E608 (Glycosyltransferase family protein 64 C3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold455G006430 PE=3 SV=1)

HSP 1 Score: 539.7 bits (1389), Expect = 8.6e-150
Identity = 271/331 (81.87%), Postives = 290/331 (87.61%), Query Frame = 0

Query: 1   MKFKSPTIFFFLLSFSAAVSS----TADRCAAEFRPDRRNLRSDQITVLINGYYESRIPL 60
           M FKS TI   LLSFS AV S    T   CAAE  PDRRNLRSDQITVLINGYYESRIPL
Sbjct: 1   MNFKSLTILVLLLSFSTAVYSLHNETTAACAAESLPDRRNLRSDQITVLINGYYESRIPL 60

Query: 61  LQSIASTYAASPFVRAVLVLWGNPSTPRETLTQLARNLTAGPISLIRQSSNSLNARFLPR 120
           LQS+A+ YAASPFV  VL+LWGNPST  +TLT+LA+NLT GPI+LIRQSSNSLN+RFLPR
Sbjct: 61  LQSLAARYAASPFVHTVLILWGNPSTSTKTLTKLAQNLTTGPITLIRQSSNSLNSRFLPR 120

Query: 121 RSIETGVVLICDDDVEIDTKSLEFAFRIWGRNPERLVGFFVRSHDIDLSKREWIYTVHPD 180
           +SI+T  VLICDDDVEIDT SLEFAFRIWGRNPERLVGFFVRSHD+DLS+REWIYTVH D
Sbjct: 121 KSIQTSAVLICDDDVEIDTPSLEFAFRIWGRNPERLVGFFVRSHDLDLSRREWIYTVHQD 180

Query: 181 KYSIVLTKLMILKTEYLFEYSCGGGEAMADMRRVVDRARNCEDILMNFVVADMSNAGPIL 240
           KYSIVLTKLMILK EYLFEYSCGGG AMADMRRVVD  RNCEDILMNFVVADMSNAGPI+
Sbjct: 181 KYSIVLTKLMILKAEYLFEYSCGGGAAMADMRRVVDLERNCEDILMNFVVADMSNAGPIM 240

Query: 241 VAAQRIRDWGDPRN--DDGDERL----GLTEVGLSNRKGQHRKRRGRCITEFHRLLGRMP 300
           VAAQRIRDWGDPRN  DDG+ERL    G +E+GLSNRKG+HRKRRGRCITEFHR LGRMP
Sbjct: 241 VAAQRIRDWGDPRNEYDDGNERLRLREGASEIGLSNRKGEHRKRRGRCITEFHRRLGRMP 300

Query: 301 LRYSYGKFVNSVGEQGLCRKGGKLVPCDQNI 322
           LRYSYGK VNS+GEQ LCRKG KLVPCDQN+
Sbjct: 301 LRYSYGKSVNSIGEQALCRKGRKLVPCDQNV 331

BLAST of MS008417 vs. ExPASy TrEMBL
Match: A0A1S3CR30 (glycosyltransferase family protein 64 C3 OS=Cucumis melo OX=3656 GN=LOC103503730 PE=3 SV=1)

HSP 1 Score: 539.7 bits (1389), Expect = 8.6e-150
Identity = 271/331 (81.87%), Postives = 290/331 (87.61%), Query Frame = 0

Query: 1   MKFKSPTIFFFLLSFSAAVSS----TADRCAAEFRPDRRNLRSDQITVLINGYYESRIPL 60
           M FKS TI   LLSFS AV S    T   CAAE  PDRRNLRSDQITVLINGYYESRIPL
Sbjct: 19  MNFKSLTILVLLLSFSTAVYSLHNETTAACAAESLPDRRNLRSDQITVLINGYYESRIPL 78

Query: 61  LQSIASTYAASPFVRAVLVLWGNPSTPRETLTQLARNLTAGPISLIRQSSNSLNARFLPR 120
           LQS+A+ YAASPFV  VL+LWGNPST  +TLT+LA+NLT GPI+LIRQSSNSLN+RFLPR
Sbjct: 79  LQSLAARYAASPFVHTVLILWGNPSTSTKTLTKLAQNLTTGPITLIRQSSNSLNSRFLPR 138

Query: 121 RSIETGVVLICDDDVEIDTKSLEFAFRIWGRNPERLVGFFVRSHDIDLSKREWIYTVHPD 180
           +SI+T  VLICDDDVEIDT SLEFAFRIWGRNPERLVGFFVRSHD+DLS+REWIYTVH D
Sbjct: 139 KSIQTSAVLICDDDVEIDTPSLEFAFRIWGRNPERLVGFFVRSHDLDLSRREWIYTVHQD 198

Query: 181 KYSIVLTKLMILKTEYLFEYSCGGGEAMADMRRVVDRARNCEDILMNFVVADMSNAGPIL 240
           KYSIVLTKLMILK EYLFEYSCGGG AMADMRRVVD  RNCEDILMNFVVADMSNAGPI+
Sbjct: 199 KYSIVLTKLMILKAEYLFEYSCGGGAAMADMRRVVDLERNCEDILMNFVVADMSNAGPIM 258

Query: 241 VAAQRIRDWGDPRN--DDGDERL----GLTEVGLSNRKGQHRKRRGRCITEFHRLLGRMP 300
           VAAQRIRDWGDPRN  DDG+ERL    G +E+GLSNRKG+HRKRRGRCITEFHR LGRMP
Sbjct: 259 VAAQRIRDWGDPRNEYDDGNERLRLREGASEIGLSNRKGEHRKRRGRCITEFHRRLGRMP 318

Query: 301 LRYSYGKFVNSVGEQGLCRKGGKLVPCDQNI 322
           LRYSYGK VNS+GEQ LCRKG KLVPCDQN+
Sbjct: 319 LRYSYGKSVNSIGEQALCRKGRKLVPCDQNV 349

BLAST of MS008417 vs. ExPASy TrEMBL
Match: A0A0A0LDW4 (Glyco_transf_64 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G883010 PE=3 SV=1)

HSP 1 Score: 538.5 bits (1386), Expect = 1.9e-149
Identity = 271/331 (81.87%), Postives = 291/331 (87.92%), Query Frame = 0

Query: 1   MKFKSPTIFFFLLSFSAAVSS----TADRCAAEFRPDRRNLRSDQITVLINGYYESRIPL 60
           M F+S TI   LLSFS AV S    TA  CAAE  PDRRNLRSDQITVLINGYYESRIPL
Sbjct: 1   MNFESLTILVLLLSFSTAVYSLHKETAAACAAESLPDRRNLRSDQITVLINGYYESRIPL 60

Query: 61  LQSIASTYAASPFVRAVLVLWGNPSTPRETLTQLARNLTAGPISLIRQSSNSLNARFLPR 120
           LQS+A+ YAASPFV  VL+LWGNPST  ETLT+LA+NLT GPISLIRQSSNSLN+RFLPR
Sbjct: 61  LQSLAARYAASPFVHTVLILWGNPSTSTETLTKLAQNLTTGPISLIRQSSNSLNSRFLPR 120

Query: 121 RSIETGVVLICDDDVEIDTKSLEFAFRIWGRNPERLVGFFVRSHDIDLSKREWIYTVHPD 180
           +SI+T  VLICDDDVEIDT SLEFAFRIWGRNPERLVGFFVRSHD+DLS+REWIYT+H D
Sbjct: 121 KSIQTFAVLICDDDVEIDTPSLEFAFRIWGRNPERLVGFFVRSHDLDLSRREWIYTIHQD 180

Query: 181 KYSIVLTKLMILKTEYLFEYSCGGGEAMADMRRVVDRARNCEDILMNFVVADMSNAGPIL 240
           KYSIVLTKLMILK EYLFEYSCGGG AMADMRRVVD  RNCEDILMNFVVADMSNAGPI+
Sbjct: 181 KYSIVLTKLMILKAEYLFEYSCGGGAAMADMRRVVDVERNCEDILMNFVVADMSNAGPIM 240

Query: 241 VAAQRIRDWGDPRN--DDGDERL----GLTEVGLSNRKGQHRKRRGRCITEFHRLLGRMP 300
           VAAQRIRDWGDPRN  DDG+ERL    G++E+GLSNRKG+HRKRRG CITEFHR LGRMP
Sbjct: 241 VAAQRIRDWGDPRNEYDDGNERLRLREGVSEIGLSNRKGEHRKRRGGCITEFHRRLGRMP 300

Query: 301 LRYSYGKFVNSVGEQGLCRKGGKLVPCDQNI 322
           LRYSYGK VNS+GEQ LCRKG KLVPCDQN+
Sbjct: 301 LRYSYGKSVNSIGEQALCRKGRKLVPCDQNV 331

BLAST of MS008417 vs. TAIR 10
Match: AT1G80290.1 (Nucleotide-diphospho-sugar transferases superfamily protein )

HSP 1 Score: 409.1 bits (1050), Expect = 3.4e-114
Identity = 201/313 (64.22%), Postives = 245/313 (78.27%), Query Frame = 0

Query: 13  LSFSAAVSSTADRCAAEFRPDRRNLRSDQITVLINGYYESRIPLLQSIASTYAASPFVRA 72
           L F   +S   D C A  + + + LRSDQITVLINGY E RIPLLQ+I ++Y++S  V +
Sbjct: 19  LVFCRTLSGDPDPCDATNQREFQKLRSDQITVLINGYSEYRIPLLQTIVASYSSSSIVSS 78

Query: 73  VLVLWGNPSTPRETLTQLARNLT-----AGPISLIRQSSNSLNARFLPRRSIETGVVLIC 132
           +LVLWGNPSTP + L QL +NLT     +  ISLI+QSS+SLNARFLPR S++T  VLIC
Sbjct: 79  ILVLWGNPSTPDQLLDQLYQNLTQYSPGSASISLIQQSSSSLNARFLPRSSVDTRAVLIC 138

Query: 133 DDDVEIDTKSLEFAFRIWGRNPERLVGFFVRSHDIDLSKREWIYTVHPDKYSIVLTKLMI 192
           DDDVEID +SLEFAF +W  NP+RLVG FVRSH  DL  +EWIYTVHPDKYSIVLTK M+
Sbjct: 139 DDDVEIDQRSLEFAFSVWKSNPDRLVGTFVRSHGFDLQGKEWIYTVHPDKYSIVLTKFMM 198

Query: 193 LKTEYLFEYSCGGGEAMADMRRVVDRARNCEDILMNFVVADMSNAGPILVAAQRIRDWGD 252
           +K +YLFEYSC GG  M +MR +VD+ RNCEDILMNFV AD   AGPI+V A+R+RDWGD
Sbjct: 199 MKQDYLFEYSCKGGVEMEEMRMIVDQMRNCEDILMNFVAADRLRAGPIMVGAERVRDWGD 258

Query: 253 PRNDDGDERLGLTEVGLSNRKGQHRKRRGRCITEFHRLLGRMPLRYSYGKFVNSVGEQGL 312
            RN++ +ER  + +VGLS+R+ +HRKRRG CI EFHR++G+MPL YSYGK VNSVGEQGL
Sbjct: 259 ARNEEVEER--VRDVGLSSRRVEHRKRRGNCIREFHRVMGKMPLMYSYGKVVNSVGEQGL 318

Query: 313 CRKGGKLVPCDQN 321
           CRK GKLV CD++
Sbjct: 319 CRKAGKLVFCDRD 329

BLAST of MS008417 vs. TAIR 10
Match: AT1G80290.2 (Nucleotide-diphospho-sugar transferases superfamily protein )

HSP 1 Score: 409.1 bits (1050), Expect = 3.4e-114
Identity = 201/313 (64.22%), Postives = 245/313 (78.27%), Query Frame = 0

Query: 13  LSFSAAVSSTADRCAAEFRPDRRNLRSDQITVLINGYYESRIPLLQSIASTYAASPFVRA 72
           L F   +S   D C A  + + + LRSDQITVLINGY E RIPLLQ+I ++Y++S  V +
Sbjct: 27  LVFCRTLSGDPDPCDATNQREFQKLRSDQITVLINGYSEYRIPLLQTIVASYSSSSIVSS 86

Query: 73  VLVLWGNPSTPRETLTQLARNLT-----AGPISLIRQSSNSLNARFLPRRSIETGVVLIC 132
           +LVLWGNPSTP + L QL +NLT     +  ISLI+QSS+SLNARFLPR S++T  VLIC
Sbjct: 87  ILVLWGNPSTPDQLLDQLYQNLTQYSPGSASISLIQQSSSSLNARFLPRSSVDTRAVLIC 146

Query: 133 DDDVEIDTKSLEFAFRIWGRNPERLVGFFVRSHDIDLSKREWIYTVHPDKYSIVLTKLMI 192
           DDDVEID +SLEFAF +W  NP+RLVG FVRSH  DL  +EWIYTVHPDKYSIVLTK M+
Sbjct: 147 DDDVEIDQRSLEFAFSVWKSNPDRLVGTFVRSHGFDLQGKEWIYTVHPDKYSIVLTKFMM 206

Query: 193 LKTEYLFEYSCGGGEAMADMRRVVDRARNCEDILMNFVVADMSNAGPILVAAQRIRDWGD 252
           +K +YLFEYSC GG  M +MR +VD+ RNCEDILMNFV AD   AGPI+V A+R+RDWGD
Sbjct: 207 MKQDYLFEYSCKGGVEMEEMRMIVDQMRNCEDILMNFVAADRLRAGPIMVGAERVRDWGD 266

Query: 253 PRNDDGDERLGLTEVGLSNRKGQHRKRRGRCITEFHRLLGRMPLRYSYGKFVNSVGEQGL 312
            RN++ +ER  + +VGLS+R+ +HRKRRG CI EFHR++G+MPL YSYGK VNSVGEQGL
Sbjct: 267 ARNEEVEER--VRDVGLSSRRVEHRKRRGNCIREFHRVMGKMPLMYSYGKVVNSVGEQGL 326

Query: 313 CRKGGKLVPCDQN 321
           CRK GKLV CD++
Sbjct: 327 CRKAGKLVFCDRD 337

BLAST of MS008417 vs. TAIR 10
Match: AT3G55830.1 (Nucleotide-diphospho-sugar transferases superfamily protein )

HSP 1 Score: 104.0 bits (258), Expect = 2.3e-22
Identity = 75/275 (27.27%), Postives = 126/275 (45.82%), Query Frame = 0

Query: 43  TVLINGYYESRIPLLQSIASTYAASPFVRAVLVLWGNPSTPRETLTQLARNLTAG----- 102
           T+L+N +   R  LL+   S YA+   + ++ ++W  P+ P E+L +   N+        
Sbjct: 75  TLLMNTW--KRYDLLKKSVSHYASCSRLDSIHIVWSEPNPPSESLKEYLHNVLKKKTRDG 134

Query: 103 ---PISLIRQSSNSLNARFLPRRSIETGVVLICDDDVEIDTKSLEFAFRIWGRNPERLVG 162
               +       +SLN RF   + ++T  V   DDD+     +++FAF +W   P+ +VG
Sbjct: 135 HEVELRFDINKEDSLNNRFKEIKDLKTDAVFSIDDDIIFPCHTVDFAFNVWESAPDTMVG 194

Query: 163 FFVRSHDIDLSKRE--------WIYTVHPDKYSIVLTKLMILKTEYLFEYSCGGGEAMAD 222
           F  R H  + S  +        W        YS+VL+K      +YL  Y+       A 
Sbjct: 195 FVPRVHWPEKSNDKANYYTYSGWWSVWWSGTYSMVLSKAAFFHKKYLSLYT---NSMPAS 254

Query: 223 MRRVVDRARNCEDILMNFVVADMSNAGPILVAAQRIRDWGDPRNDDGDERLGLTEVGLSN 282
           +R    + RNCEDI M+F++A+ +NA P +    +I + G           G++ +    
Sbjct: 255 IREFTTKNRNCEDIAMSFLIANATNA-PAIWVKGKIYEIGS---------TGISSI---- 314

Query: 283 RKGQHRKRRGRCITEFHRLLGRMPLRYSYGKFVNS 302
             G H ++R  C+  F    G+MPL Y+  K V+S
Sbjct: 315 --GGHTEKRTHCVNRFVAEFGKMPLVYTSMKAVDS 328

BLAST of MS008417 vs. TAIR 10
Match: AT5G04500.1 (glycosyltransferase family protein 47 )

HSP 1 Score: 101.3 bits (251), Expect = 1.5e-21
Identity = 70/248 (28.23%), Postives = 114/248 (45.97%), Query Frame = 0

Query: 50  YESRIPLLQSIASTYAASPFVRAVLVLWGNPSTPRETLTQLARNLTAGPISLIRQSSNSL 109
           Y++R+  L+     Y+  P V+ ++V+W     P      L+   +A P+ +  Q  NSL
Sbjct: 525 YDARLWNLKMYVKRYSRCPSVKEIVVIWNKGPPP-----DLSELDSAVPVRIRVQKQNSL 584

Query: 110 NARFLPRRSIETGVVLICDDDVEIDTKSLEFAFRIWGRNPERLVGFFVRSHDIDLSKREW 169
           N RF     I+T  VL  DDD+ +    +E  FR+W  +PERLVGF+ R  D  ++    
Sbjct: 585 NNRFEIDPLIKTRAVLELDDDIMMPCDDIEKGFRVWREHPERLVGFYPRFVDQTMTYSAE 644

Query: 170 IYTVHPDKYSIVLTKLMILKTEYLFEYSCGGGEAMADMRRVVDRARNCEDILMNFVVADM 229
            +      Y+++LT    +   + F+        +   R  VD   NCEDIL+NF+ A+ 
Sbjct: 645 KFARSHKGYNMILTGAAFMDVRFAFDMYQSDKAKLG--RVFVDEQFNCEDILLNFLYANA 704

Query: 230 SNAGPILVAAQRIRDWGDPRNDDGDERLGLTEVGLSNRKGQHRKRRGRCITEFHRLLGRM 289
           S +G  +   +      D     G        V +S    QH ++R +C+  F  L G +
Sbjct: 705 SGSGKAVEYVRPSLVTIDTSKFSG--------VAISGNTNQHYRKRSKCLRRFSDLYGSL 757

Query: 290 -PLRYSYG 297
              R+ +G
Sbjct: 765 VDRRWEFG 757

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022137713.13.0e-18199.38glycosyltransferase family protein 64 C3 [Momordica charantia][more]
XP_038898918.19.4e-15183.08glycosyltransferase family protein 64 C3 [Benincasa hispida][more]
KAG7024528.19.4e-15183.33Glycosyltransferase family protein 64 C3, partial [Cucurbita argyrosperma subsp.... [more]
KAG6591645.19.4e-15183.33Glycosyltransferase family protein 64 C3, partial [Cucurbita argyrosperma subsp.... [more]
XP_022975936.12.7e-15083.33glycosyltransferase family protein 64 C3 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q9C9754.7e-11364.22Glycosyltransferase family protein 64 C3 OS=Arabidopsis thaliana OX=3702 GN=At1g... [more]
Q9WVL63.4e-2632.18Exostosin-like 3 OS=Mus musculus OX=10090 GN=Extl3 PE=1 SV=2[more]
O439097.5e-2632.18Exostosin-like 3 OS=Homo sapiens OX=9606 GN=EXTL3 PE=1 SV=1[more]
Q5RBC33.5e-2332.93Exostosin-1 OS=Pongo abelii OX=9601 GN=EXT1 PE=2 SV=1[more]
Q163944.5e-2332.78Exostosin-1 OS=Homo sapiens OX=9606 GN=EXT1 PE=1 SV=2[more]
Match NameE-valueIdentityDescription
A0A6J1CB501.4e-18199.38glycosyltransferase family protein 64 C3 OS=Momordica charantia OX=3673 GN=LOC11... [more]
A0A6J1IFJ61.3e-15083.33glycosyltransferase family protein 64 C3 OS=Cucurbita maxima OX=3661 GN=LOC11147... [more]
A0A5D3E6088.6e-15081.87Glycosyltransferase family protein 64 C3 OS=Cucumis melo var. makuwa OX=1194695 ... [more]
A0A1S3CR308.6e-15081.87glycosyltransferase family protein 64 C3 OS=Cucumis melo OX=3656 GN=LOC103503730... [more]
A0A0A0LDW41.9e-14981.87Glyco_transf_64 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G88... [more]
Match NameE-valueIdentityDescription
AT1G80290.13.4e-11464.22Nucleotide-diphospho-sugar transferases superfamily protein [more]
AT1G80290.23.4e-11464.22Nucleotide-diphospho-sugar transferases superfamily protein [more]
AT3G55830.12.3e-2227.27Nucleotide-diphospho-sugar transferases superfamily protein [more]
AT5G04500.11.5e-2128.23glycosyltransferase family protein 47 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR015338Glycosyl transferase 64 domainPFAMPF09258Glyco_transf_64coord: 42..300
e-value: 3.3E-80
score: 268.9
IPR029044Nucleotide-diphospho-sugar transferasesGENE3D3.90.550.10Spore Coat Polysaccharide Biosynthesis Protein SpsA; Chain Acoord: 38..305
e-value: 3.3E-77
score: 261.0
IPR029044Nucleotide-diphospho-sugar transferasesSUPERFAMILY53448Nucleotide-diphospho-sugar transferasescoord: 42..295
NoneNo IPR availablePANTHERPTHR11062:SF254GLYCOSYLTRANSFERASE FAMILY PROTEIN 64 C3coord: 22..317
IPR004263Exostosin-likePANTHERPTHR11062EXOSTOSIN HEPARAN SULFATE GLYCOSYLTRANSFERASE -RELATEDcoord: 22..317

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS008417.1MS008417.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006486 protein glycosylation
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0016757 glycosyltransferase activity