MS008269 (gene) Bitter gourd (TR) v1

Overview
NameMS008269
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionARID domain-containing protein
Locationscaffold4: 56185 .. 57135 (-)
RNA-Seq ExpressionMS008269
SyntenyMS008269
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAATTGGGCAGAGATGCTAAAGCGATCCATTCGGCGGATTTGTTGGTGTGTTTTCCTTCTCGATCGAATCTGACTTTAATGCCGAAGCCGCTTTGTAGTCCAGCGAGGGGATTGGATTCCAACAAGCTCCGCCGCAGCCACCGCCACCACCATCGCCGGAGGAAATCGACCTCCGCCGCCAGTCCGCTGATATGGGCGAAACCCAAGACAATGGGGTCCGAGATATCGGAGCCGTCGTCCCCGAAAGTGACGTGTGCTGGGCAGATAAAGATCAGGCCCAAGACCGGCTCCTGCAAGAGCTGGCAATCGGTGATGGAAGAGATTGAGAGAATTCACAACAGGAGGAAATTACGGAGGAGGAGGTCTAATTGGGTCGAGTCTTTGGGGTTCAAGAAAGATATAATGCAATTCTTGACGTGTTTACGGAACATCCGGTTTGATTTTCGGTGTTTCAAAGCTTTCCCAGAAGCAGATTTCACTACGGAAGAAGAAGATGAAGAAGAGGAGGAGGAAGAAGAGGAGGGAAAATCTCAGGAAAATCAAGTGGGAGTTGAGGGAAATGAAAGCTCCAGAACTGCATTTTCTAAATGGTTTATGGTTTTACAGGAAAGTGGGGCTAGTAATGGGATTTGCAGAGAAAGCAATGGCCCGCCATTGGCACCTCCCAAGAATGCTCTTTTGCTAATGCGATGCAGGTCTGCTCCAGCAAAGAGTTGGCAGGAGGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGCAGCAGAAGAAGATGAAAAGGAAGTGAAGGTGAAGAAGAGCTTGAAATGGCTAATGGAGGAAGAGAACAGAGAGAGATTGGTTATGGAAATGGGGCCTGATTTCTGCAGAATGTCATCGGAGATTGCAAAGGAGACATGGGTTGGCAGGGATTTGTTTTCAAGGAGTCGGAGTTGGAAGGCT

mRNA sequence

ATGAAATTGGGCAGAGATGCTAAAGCGATCCATTCGGCGGATTTGTTGGTGTGTTTTCCTTCTCGATCGAATCTGACTTTAATGCCGAAGCCGCTTTGTAGTCCAGCGAGGGGATTGGATTCCAACAAGCTCCGCCGCAGCCACCGCCACCACCATCGCCGGAGGAAATCGACCTCCGCCGCCAGTCCGCTGATATGGGCGAAACCCAAGACAATGGGGTCCGAGATATCGGAGCCGTCGTCCCCGAAAGTGACGTGTGCTGGGCAGATAAAGATCAGGCCCAAGACCGGCTCCTGCAAGAGCTGGCAATCGGTGATGGAAGAGATTGAGAGAATTCACAACAGGAGGAAATTACGGAGGAGGAGGTCTAATTGGGTCGAGTCTTTGGGGTTCAAGAAAGATATAATGCAATTCTTGACGTGTTTACGGAACATCCGGTTTGATTTTCGGTGTTTCAAAGCTTTCCCAGAAGCAGATTTCACTACGGAAGAAGAAGATGAAGAAGAGGAGGAGGAAGAAGAGGAGGGAAAATCTCAGGAAAATCAAGTGGGAGTTGAGGGAAATGAAAGCTCCAGAACTGCATTTTCTAAATGGTTTATGGTTTTACAGGAAAGTGGGGCTAGTAATGGGATTTGCAGAGAAAGCAATGGCCCGCCATTGGCACCTCCCAAGAATGCTCTTTTGCTAATGCGATGCAGGTCTGCTCCAGCAAAGAGTTGGCAGGAGGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGCAGCAGAAGAAGATGAAAAGGAAGTGAAGGTGAAGAAGAGCTTGAAATGGCTAATGGAGGAAGAGAACAGAGAGAGATTGGTTATGGAAATGGGGCCTGATTTCTGCAGAATGTCATCGGAGATTGCAAAGGAGACATGGGTTGGCAGGGATTTGTTTTCAAGGAGTCGGAGTTGGAAGGCT

Coding sequence (CDS)

ATGAAATTGGGCAGAGATGCTAAAGCGATCCATTCGGCGGATTTGTTGGTGTGTTTTCCTTCTCGATCGAATCTGACTTTAATGCCGAAGCCGCTTTGTAGTCCAGCGAGGGGATTGGATTCCAACAAGCTCCGCCGCAGCCACCGCCACCACCATCGCCGGAGGAAATCGACCTCCGCCGCCAGTCCGCTGATATGGGCGAAACCCAAGACAATGGGGTCCGAGATATCGGAGCCGTCGTCCCCGAAAGTGACGTGTGCTGGGCAGATAAAGATCAGGCCCAAGACCGGCTCCTGCAAGAGCTGGCAATCGGTGATGGAAGAGATTGAGAGAATTCACAACAGGAGGAAATTACGGAGGAGGAGGTCTAATTGGGTCGAGTCTTTGGGGTTCAAGAAAGATATAATGCAATTCTTGACGTGTTTACGGAACATCCGGTTTGATTTTCGGTGTTTCAAAGCTTTCCCAGAAGCAGATTTCACTACGGAAGAAGAAGATGAAGAAGAGGAGGAGGAAGAAGAGGAGGGAAAATCTCAGGAAAATCAAGTGGGAGTTGAGGGAAATGAAAGCTCCAGAACTGCATTTTCTAAATGGTTTATGGTTTTACAGGAAAGTGGGGCTAGTAATGGGATTTGCAGAGAAAGCAATGGCCCGCCATTGGCACCTCCCAAGAATGCTCTTTTGCTAATGCGATGCAGGTCTGCTCCAGCAAAGAGTTGGCAGGAGGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGCAGCAGAAGAAGATGAAAAGGAAGTGAAGGTGAAGAAGAGCTTGAAATGGCTAATGGAGGAAGAGAACAGAGAGAGATTGGTTATGGAAATGGGGCCTGATTTCTGCAGAATGTCATCGGAGATTGCAAAGGAGACATGGGTTGGCAGGGATTTGTTTTCAAGGAGTCGGAGTTGGAAGGCT

Protein sequence

MKLGRDAKAIHSADLLVCFPSRSNLTLMPKPLCSPARGLDSNKLRRSHRHHHRRRKSTSAASPLIWAKPKTMGSEISEPSSPKVTCAGQIKIRPKTGSCKSWQSVMEEIERIHNRRKLRRRRSNWVESLGFKKDIMQFLTCLRNIRFDFRCFKAFPEADFTTEEEDEEEEEEEEEGKSQENQVGVEGNESSRTAFSKWFMVLQESGASNGICRESNGPPLAPPKNALLLMRCRSAPAKSWQEEEEEEEEEEEEEEEAAEEDEKEVKVKKSLKWLMEEENRERLVMEMGPDFCRMSSEIAKETWVGRDLFSRSRSWKA
Homology
BLAST of MS008269 vs. NCBI nr
Match: XP_022147766.1 (uncharacterized protein LOC111016595 [Momordica charantia])

HSP 1 Score: 594.0 bits (1530), Expect = 7.8e-166
Identity = 316/322 (98.14%), Postives = 316/322 (98.14%), Query Frame = 0

Query: 1   MKLGRDAKAIHSADLLVCFPSRSNLTLMPKPLCSPARGLDSNKLRRSHRHHHRRRKSTSA 60
           MKLGRDAKAIHSADLLVCFPSRSNLTLMPKPLCSPARGLDSNKLRRSHRHHHRRRKSTSA
Sbjct: 1   MKLGRDAKAIHSADLLVCFPSRSNLTLMPKPLCSPARGLDSNKLRRSHRHHHRRRKSTSA 60

Query: 61  ASPLIWAKPKTMGSEISEPSSPKVTCAGQIKIRPKTGSCKSWQSVMEEIERIHNRRKLRR 120
           ASPLIWAKPKTMGSEISEPSSPKVTCAGQIKIRPKTGSCKSWQSVMEEIERIHNRRKLRR
Sbjct: 61  ASPLIWAKPKTMGSEISEPSSPKVTCAGQIKIRPKTGSCKSWQSVMEEIERIHNRRKLRR 120

Query: 121 RRSNWVESLGFKKDIMQFLTCLRNIRFDFRCFKAFPEADFTTEEEDEEEEEEEEEGKSQE 180
           RRSNWVESLGFKKDIMQFLTCLRNIRFDFRCFKAFPEADFTTEEED EEEEEEEEGKSQE
Sbjct: 121 RRSNWVESLGFKKDIMQFLTCLRNIRFDFRCFKAFPEADFTTEEED-EEEEEEEEGKSQE 180

Query: 181 NQVGVEGNESSRTAFSKWFMVLQESGASNGICRESNGPPLAPPKNALLLMRCRSAPAKSW 240
           NQVGVEGNESSRTAFSKWFMVLQESGASNGICRESNGPPLAPPKNALLLMRCRSAPAKSW
Sbjct: 181 NQVGVEGNESSRTAFSKWFMVLQESGASNGICRESNGPPLAPPKNALLLMRCRSAPAKSW 240

Query: 241 QEEEEEEEEEEEEEEE-----AAEEDEKEVKVKKSLKWLMEEENRERLVMEMGPDFCRMS 300
           QEEEEEEEEEEEEEEE     AAEEDEKEVKVKKSLKWLMEEENRERLVMEMGPDFCRMS
Sbjct: 241 QEEEEEEEEEEEEEEEEEEEAAAEEDEKEVKVKKSLKWLMEEENRERLVMEMGPDFCRMS 300

Query: 301 SEIAKETWVGRDLFSRSRSWKA 318
           SEIAKETWVGRDLFSRSRSWKA
Sbjct: 301 SEIAKETWVGRDLFSRSRSWKA 321

BLAST of MS008269 vs. NCBI nr
Match: XP_038898663.1 (transcription initiation factor IIE subunit alpha [Benincasa hispida])

HSP 1 Score: 438.0 bits (1125), Expect = 7.1e-119
Identity = 249/330 (75.45%), Postives = 276/330 (83.64%), Query Frame = 0

Query: 1   MKLGRDAKAIHSADLLVCFPSRSNLTLMPKPLCSPARGLDSNKLRRSHRHHHRRRKSTSA 60
           MKLGR+ K I SADLLVCFPSRS+L LMP PLCSPARG DS+K R SHRH+HRRRK  SA
Sbjct: 1   MKLGRENKGIPSADLLVCFPSRSHLALMPNPLCSPARGSDSSKFRLSHRHYHRRRK--SA 60

Query: 61  ASPLIWAKPKTMGSEISEPSSPKVTCAGQIKIRPKTGSCKSWQSVMEEIERIHNRRKLRR 120
            SP++WAK KTMGSEISEPSSPKVTCAGQIKIRPK  + KSWQSVMEEIERIHNRRKLRR
Sbjct: 61  ESPVVWAKAKTMGSEISEPSSPKVTCAGQIKIRPK--NSKSWQSVMEEIERIHNRRKLRR 120

Query: 121 RRSNWVESLGFKKDIMQFLTCLRNIRFDFRCFKAFPEADFTTEEEDEEEEEEEEEGKSQE 180
           RR +WVESLGFKKDIMQFLTCLRNIRFDFRCF+AFP  DFTTEEE+EEEEEEEE  KSQ 
Sbjct: 121 RRFHWVESLGFKKDIMQFLTCLRNIRFDFRCFRAFPATDFTTEEEEEEEEEEEE--KSQG 180

Query: 181 NQVGVEGNESSRTAFSKWFMVLQESGASNGICRESN----------GPPLAPPKNALLLM 240
           NQVGV+ NESSRTAFSKWFMVLQE+G SN + RES              +APPKNALLLM
Sbjct: 181 NQVGVDENESSRTAFSKWFMVLQENG-SNELKRESKILCSDDDVSIEAAMAPPKNALLLM 240

Query: 241 RCRSAPAKSWQEEEEEEEEEEEEEEEAAEEDEKEVKVKKSLKWLMEEENRERLVMEMGPD 300
           RCRSAPAK W EEE EEEE+++++    +++++EVKVKKSLKWLMEEENRERLVME G D
Sbjct: 241 RCRSAPAKRWLEEESEEEEDDDDD----DDEKEEVKVKKSLKWLMEEENRERLVMETGTD 300

Query: 301 FCRMSSEIAKETWV----GRDLFSRSRSWK 317
           FCRM+S+IAKETWV     RDLF+RS SWK
Sbjct: 301 FCRMTSDIAKETWVVSEKSRDLFTRSHSWK 319

BLAST of MS008269 vs. NCBI nr
Match: XP_004142611.2 (transcription initiation factor IIE subunit alpha [Cucumis sativus] >KAE8649637.1 hypothetical protein Csa_012410 [Cucumis sativus])

HSP 1 Score: 403.3 bits (1035), Expect = 2.0e-108
Identity = 231/327 (70.64%), Postives = 268/327 (81.96%), Query Frame = 0

Query: 1   MKLGRD-AKAIHSADLLVCFPSRSNLTLMPKPLCSPARGLDSNKLRRSHRHHHRRRKSTS 60
           MKL R+ +K I S+DLLVCFPSRS+L LMP PLCSPARG DS+K R  +R +HRRRK  S
Sbjct: 11  MKLNREKSKGIPSSDLLVCFPSRSHLALMPNPLCSPARGSDSSKFRLDYRRYHRRRK--S 70

Query: 61  AASPLIWAKPKTMGSEISEPSSPKVTCAGQIKIRPKTGSCKSWQSVMEEIERIHNRRKLR 120
           A SP++WAK KTMGSEISEPSSPKVTCAGQIKIRPK  + KSWQSVMEEIERIHNRRKLR
Sbjct: 71  AESPVVWAKAKTMGSEISEPSSPKVTCAGQIKIRPK--NSKSWQSVMEEIERIHNRRKLR 130

Query: 121 RRRSNWVESLGFKKDIMQFLTCLRNIRFDFRCFKAFPEADFTTEEEDEEEEEEEEEGKSQ 180
           RRR NW+ES GFKKDIMQFLTCLR +RFDFRCF+AFPE DFTTEEE+EEEEEEEEE   +
Sbjct: 131 RRRFNWIESFGFKKDIMQFLTCLRTMRFDFRCFRAFPETDFTTEEEEEEEEEEEEE--EE 190

Query: 181 ENQVGVEGNESSRTAFSKWFMVLQESGASNGICRESNG----------PPLAPPKNALLL 240
           +NQVG+E NESSRTAFSKWFMVLQE+G SN + R+SN             +APP+NALLL
Sbjct: 191 KNQVGIEENESSRTAFSKWFMVLQENG-SNELKRDSNSRCYEDDESIEATMAPPRNALLL 250

Query: 241 MRCRSAPAKSWQEEEEEEEEEEEEEEEAAEEDEKEVKVKKSLKWLMEEENRERLVMEMGP 300
           MRC+SAPA+ W EEE EEE++E+E+    E+++++VKVKKSLKWLMEEENRER+VMEMG 
Sbjct: 251 MRCKSAPARRWMEEESEEEDDEKEK----EKEKEKVKVKKSLKWLMEEENRERVVMEMGT 310

Query: 301 DFCRMSSEIAKETWVGRDLFSRSRSWK 317
           DFCRM S+ AKE       F+RS+SWK
Sbjct: 311 DFCRMISDNAKE-------FTRSQSWK 319

BLAST of MS008269 vs. NCBI nr
Match: XP_008444111.1 (PREDICTED: uncharacterized protein LOC103487551 [Cucumis melo] >KAA0064246.1 transcription initiation factor IIE subunit alpha-like [Cucumis melo var. makuwa] >TYK18616.1 transcription initiation factor IIE subunit alpha-like [Cucumis melo var. makuwa])

HSP 1 Score: 397.5 bits (1020), Expect = 1.1e-106
Identity = 231/326 (70.86%), Postives = 257/326 (78.83%), Query Frame = 0

Query: 1   MKLGRD-AKAIHSADLLVCFPSRSNLTLMPKPLCSPARGLDSNKLRRSHRHHHRRRKSTS 60
           MKL R+ +K I SADLLVCFPSRS+L LMP PLCSPARG DS+K R  HR  HRRRK  S
Sbjct: 8   MKLNREKSKGIPSADLLVCFPSRSHLALMPNPLCSPARGSDSSKFRLDHRRFHRRRK--S 67

Query: 61  AASPLIWAKPKTMGSEISEPSSPKVTCAGQIKIRPKTGSCKSWQSVMEEIERIHNRRKLR 120
           A SP++WAK KTMGSEISEPSSPKVTCAGQIKIRPK  + KSWQSVMEEIERIHNRRKLR
Sbjct: 68  AESPVVWAKAKTMGSEISEPSSPKVTCAGQIKIRPK--NSKSWQSVMEEIERIHNRRKLR 127

Query: 121 RRRSNWVESLGFKKDIMQFLTCLRNIRFDFRCFKAFPEADFTTEEEDEEEEEEEEEGKSQ 180
           RRR  WVES GFKKDIMQFLTCLR IRFDFRCF+AFPE DFTTEEE+EEEEEEE+E    
Sbjct: 128 RRRFRWVESFGFKKDIMQFLTCLRTIRFDFRCFRAFPETDFTTEEEEEEEEEEEDE---- 187

Query: 181 ENQVGVEGNESSRTAFSKWFMVLQESGA------SNGICR---ESNGPPLAPPKNALLLM 240
           +NQVG+E NESSRTAFSKWFMVLQE+G+      S  +C    ES    +APP NALLLM
Sbjct: 188 KNQVGIEENESSRTAFSKWFMVLQENGSNELKRDSKSLCNEDDESIEAIMAPPINALLLM 247

Query: 241 RCRSAPAKSWQEEEEEEEEEEEEEEEAAEEDEKEVKVKKSLKWLMEEENRERLVMEMGPD 300
           RCRSAPA+ W EEE EE ++E+E          +VKVKKSLKWLMEEENRERLV+EMG D
Sbjct: 248 RCRSAPARRWMEEESEEGDDEKE----------KVKVKKSLKWLMEEENRERLVVEMGTD 307

Query: 301 FCRMSSEIAKETWVGRDLFSRSRSWK 317
           FCRM+S+ AKE       F+RS+SWK
Sbjct: 308 FCRMTSDNAKE-------FTRSQSWK 308

BLAST of MS008269 vs. NCBI nr
Match: XP_023523446.1 (uncharacterized protein LOC111787660 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 360.1 bits (923), Expect = 1.9e-95
Identity = 211/318 (66.35%), Postives = 236/318 (74.21%), Query Frame = 0

Query: 1   MKLGRDAKAIHSADLLVCFPSRSNLTLMPKPLCSPARGLDSNKLRRSHR-HHHRRRKSTS 60
           MKLGR+AK I S DLLVCFPSRS L LMP PLCSP RG DS+KLRRS R +HHRRRK  S
Sbjct: 68  MKLGREAKGISSTDLLVCFPSRSRLALMPNPLCSPVRGSDSSKLRRSRRSYHHRRRK--S 127

Query: 61  AASPLIWAKPKTMGSEISEPSSPKVTCAGQIKIRPKTGSCKSWQSVMEEIERIHNRRKLR 120
           A SP++WAK KTMGSE+SEPSSPKVTCAGQ+K+R K  + + WQSVMEEIERIHNRRKLR
Sbjct: 128 AESPVVWAKAKTMGSEMSEPSSPKVTCAGQVKMRRK--NRERWQSVMEEIERIHNRRKLR 187

Query: 121 RRRS-NWVESLGFKKDIMQFLTCLRNIRFDFRCFKAFPEADFTTEEEDEEEEEEEEEGKS 180
           RRR  NWVESLGFKKDIMQ LTCLR++RFDFRCF+AFPEADFTTEE+++EEEEEEEE + 
Sbjct: 188 RRRRVNWVESLGFKKDIMQMLTCLRSLRFDFRCFRAFPEADFTTEEDEDEEEEEEEEEEE 247

Query: 181 QENQVGVEGNESSRTAFSKWFMVLQESGASNGICRESNGPPLAPPKNALLLMRCRSAPAK 240
           +E       +ESSR AFSKWFMVLQE+   NG C   +  P+APPKNALLLMRCRSAPAK
Sbjct: 248 EE-------SESSRAAFSKWFMVLQENRMMNG-CAVDDDAPVAPPKNALLLMRCRSAPAK 307

Query: 241 SWQEEEEEEEEEEEEEEEAAEEDEKEVKVKKSLKWLMEEENRERLVMEMGPDFCRMSSEI 300
           SW E                   EK  K KKSLKWLMEEENRERL ME G +  R S   
Sbjct: 308 SWVE-------------------EKVKKKKKSLKWLMEEENRERLAMEKGTEISRTS--- 344

Query: 301 AKETWVGRDLFSRSRSWK 317
               W+     +RSRSWK
Sbjct: 368 --PYWL-----TRSRSWK 344

BLAST of MS008269 vs. ExPASy TrEMBL
Match: A0A6J1D3C2 (uncharacterized protein LOC111016595 OS=Momordica charantia OX=3673 GN=LOC111016595 PE=4 SV=1)

HSP 1 Score: 594.0 bits (1530), Expect = 3.8e-166
Identity = 316/322 (98.14%), Postives = 316/322 (98.14%), Query Frame = 0

Query: 1   MKLGRDAKAIHSADLLVCFPSRSNLTLMPKPLCSPARGLDSNKLRRSHRHHHRRRKSTSA 60
           MKLGRDAKAIHSADLLVCFPSRSNLTLMPKPLCSPARGLDSNKLRRSHRHHHRRRKSTSA
Sbjct: 1   MKLGRDAKAIHSADLLVCFPSRSNLTLMPKPLCSPARGLDSNKLRRSHRHHHRRRKSTSA 60

Query: 61  ASPLIWAKPKTMGSEISEPSSPKVTCAGQIKIRPKTGSCKSWQSVMEEIERIHNRRKLRR 120
           ASPLIWAKPKTMGSEISEPSSPKVTCAGQIKIRPKTGSCKSWQSVMEEIERIHNRRKLRR
Sbjct: 61  ASPLIWAKPKTMGSEISEPSSPKVTCAGQIKIRPKTGSCKSWQSVMEEIERIHNRRKLRR 120

Query: 121 RRSNWVESLGFKKDIMQFLTCLRNIRFDFRCFKAFPEADFTTEEEDEEEEEEEEEGKSQE 180
           RRSNWVESLGFKKDIMQFLTCLRNIRFDFRCFKAFPEADFTTEEED EEEEEEEEGKSQE
Sbjct: 121 RRSNWVESLGFKKDIMQFLTCLRNIRFDFRCFKAFPEADFTTEEED-EEEEEEEEGKSQE 180

Query: 181 NQVGVEGNESSRTAFSKWFMVLQESGASNGICRESNGPPLAPPKNALLLMRCRSAPAKSW 240
           NQVGVEGNESSRTAFSKWFMVLQESGASNGICRESNGPPLAPPKNALLLMRCRSAPAKSW
Sbjct: 181 NQVGVEGNESSRTAFSKWFMVLQESGASNGICRESNGPPLAPPKNALLLMRCRSAPAKSW 240

Query: 241 QEEEEEEEEEEEEEEE-----AAEEDEKEVKVKKSLKWLMEEENRERLVMEMGPDFCRMS 300
           QEEEEEEEEEEEEEEE     AAEEDEKEVKVKKSLKWLMEEENRERLVMEMGPDFCRMS
Sbjct: 241 QEEEEEEEEEEEEEEEEEEEAAAEEDEKEVKVKKSLKWLMEEENRERLVMEMGPDFCRMS 300

Query: 301 SEIAKETWVGRDLFSRSRSWKA 318
           SEIAKETWVGRDLFSRSRSWKA
Sbjct: 301 SEIAKETWVGRDLFSRSRSWKA 321

BLAST of MS008269 vs. ExPASy TrEMBL
Match: A0A0A0L1Z4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G377750 PE=4 SV=1)

HSP 1 Score: 403.3 bits (1035), Expect = 9.4e-109
Identity = 231/327 (70.64%), Postives = 268/327 (81.96%), Query Frame = 0

Query: 1   MKLGRD-AKAIHSADLLVCFPSRSNLTLMPKPLCSPARGLDSNKLRRSHRHHHRRRKSTS 60
           MKL R+ +K I S+DLLVCFPSRS+L LMP PLCSPARG DS+K R  +R +HRRRK  S
Sbjct: 1   MKLNREKSKGIPSSDLLVCFPSRSHLALMPNPLCSPARGSDSSKFRLDYRRYHRRRK--S 60

Query: 61  AASPLIWAKPKTMGSEISEPSSPKVTCAGQIKIRPKTGSCKSWQSVMEEIERIHNRRKLR 120
           A SP++WAK KTMGSEISEPSSPKVTCAGQIKIRPK  + KSWQSVMEEIERIHNRRKLR
Sbjct: 61  AESPVVWAKAKTMGSEISEPSSPKVTCAGQIKIRPK--NSKSWQSVMEEIERIHNRRKLR 120

Query: 121 RRRSNWVESLGFKKDIMQFLTCLRNIRFDFRCFKAFPEADFTTEEEDEEEEEEEEEGKSQ 180
           RRR NW+ES GFKKDIMQFLTCLR +RFDFRCF+AFPE DFTTEEE+EEEEEEEEE   +
Sbjct: 121 RRRFNWIESFGFKKDIMQFLTCLRTMRFDFRCFRAFPETDFTTEEEEEEEEEEEEE--EE 180

Query: 181 ENQVGVEGNESSRTAFSKWFMVLQESGASNGICRESNG----------PPLAPPKNALLL 240
           +NQVG+E NESSRTAFSKWFMVLQE+G SN + R+SN             +APP+NALLL
Sbjct: 181 KNQVGIEENESSRTAFSKWFMVLQENG-SNELKRDSNSRCYEDDESIEATMAPPRNALLL 240

Query: 241 MRCRSAPAKSWQEEEEEEEEEEEEEEEAAEEDEKEVKVKKSLKWLMEEENRERLVMEMGP 300
           MRC+SAPA+ W EEE EEE++E+E+    E+++++VKVKKSLKWLMEEENRER+VMEMG 
Sbjct: 241 MRCKSAPARRWMEEESEEEDDEKEK----EKEKEKVKVKKSLKWLMEEENRERVVMEMGT 300

Query: 301 DFCRMSSEIAKETWVGRDLFSRSRSWK 317
           DFCRM S+ AKE       F+RS+SWK
Sbjct: 301 DFCRMISDNAKE-------FTRSQSWK 309

BLAST of MS008269 vs. ExPASy TrEMBL
Match: A0A5D3D503 (Transcription initiation factor IIE subunit alpha-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold119G001490 PE=4 SV=1)

HSP 1 Score: 397.5 bits (1020), Expect = 5.2e-107
Identity = 231/326 (70.86%), Postives = 257/326 (78.83%), Query Frame = 0

Query: 1   MKLGRD-AKAIHSADLLVCFPSRSNLTLMPKPLCSPARGLDSNKLRRSHRHHHRRRKSTS 60
           MKL R+ +K I SADLLVCFPSRS+L LMP PLCSPARG DS+K R  HR  HRRRK  S
Sbjct: 8   MKLNREKSKGIPSADLLVCFPSRSHLALMPNPLCSPARGSDSSKFRLDHRRFHRRRK--S 67

Query: 61  AASPLIWAKPKTMGSEISEPSSPKVTCAGQIKIRPKTGSCKSWQSVMEEIERIHNRRKLR 120
           A SP++WAK KTMGSEISEPSSPKVTCAGQIKIRPK  + KSWQSVMEEIERIHNRRKLR
Sbjct: 68  AESPVVWAKAKTMGSEISEPSSPKVTCAGQIKIRPK--NSKSWQSVMEEIERIHNRRKLR 127

Query: 121 RRRSNWVESLGFKKDIMQFLTCLRNIRFDFRCFKAFPEADFTTEEEDEEEEEEEEEGKSQ 180
           RRR  WVES GFKKDIMQFLTCLR IRFDFRCF+AFPE DFTTEEE+EEEEEEE+E    
Sbjct: 128 RRRFRWVESFGFKKDIMQFLTCLRTIRFDFRCFRAFPETDFTTEEEEEEEEEEEDE---- 187

Query: 181 ENQVGVEGNESSRTAFSKWFMVLQESGA------SNGICR---ESNGPPLAPPKNALLLM 240
           +NQVG+E NESSRTAFSKWFMVLQE+G+      S  +C    ES    +APP NALLLM
Sbjct: 188 KNQVGIEENESSRTAFSKWFMVLQENGSNELKRDSKSLCNEDDESIEAIMAPPINALLLM 247

Query: 241 RCRSAPAKSWQEEEEEEEEEEEEEEEAAEEDEKEVKVKKSLKWLMEEENRERLVMEMGPD 300
           RCRSAPA+ W EEE EE ++E+E          +VKVKKSLKWLMEEENRERLV+EMG D
Sbjct: 248 RCRSAPARRWMEEESEEGDDEKE----------KVKVKKSLKWLMEEENRERLVVEMGTD 307

Query: 301 FCRMSSEIAKETWVGRDLFSRSRSWK 317
           FCRM+S+ AKE       F+RS+SWK
Sbjct: 308 FCRMTSDNAKE-------FTRSQSWK 308

BLAST of MS008269 vs. ExPASy TrEMBL
Match: A0A1S3B949 (uncharacterized protein LOC103487551 OS=Cucumis melo OX=3656 GN=LOC103487551 PE=4 SV=1)

HSP 1 Score: 397.5 bits (1020), Expect = 5.2e-107
Identity = 231/326 (70.86%), Postives = 257/326 (78.83%), Query Frame = 0

Query: 1   MKLGRD-AKAIHSADLLVCFPSRSNLTLMPKPLCSPARGLDSNKLRRSHRHHHRRRKSTS 60
           MKL R+ +K I SADLLVCFPSRS+L LMP PLCSPARG DS+K R  HR  HRRRK  S
Sbjct: 8   MKLNREKSKGIPSADLLVCFPSRSHLALMPNPLCSPARGSDSSKFRLDHRRFHRRRK--S 67

Query: 61  AASPLIWAKPKTMGSEISEPSSPKVTCAGQIKIRPKTGSCKSWQSVMEEIERIHNRRKLR 120
           A SP++WAK KTMGSEISEPSSPKVTCAGQIKIRPK  + KSWQSVMEEIERIHNRRKLR
Sbjct: 68  AESPVVWAKAKTMGSEISEPSSPKVTCAGQIKIRPK--NSKSWQSVMEEIERIHNRRKLR 127

Query: 121 RRRSNWVESLGFKKDIMQFLTCLRNIRFDFRCFKAFPEADFTTEEEDEEEEEEEEEGKSQ 180
           RRR  WVES GFKKDIMQFLTCLR IRFDFRCF+AFPE DFTTEEE+EEEEEEE+E    
Sbjct: 128 RRRFRWVESFGFKKDIMQFLTCLRTIRFDFRCFRAFPETDFTTEEEEEEEEEEEDE---- 187

Query: 181 ENQVGVEGNESSRTAFSKWFMVLQESGA------SNGICR---ESNGPPLAPPKNALLLM 240
           +NQVG+E NESSRTAFSKWFMVLQE+G+      S  +C    ES    +APP NALLLM
Sbjct: 188 KNQVGIEENESSRTAFSKWFMVLQENGSNELKRDSKSLCNEDDESIEAIMAPPINALLLM 247

Query: 241 RCRSAPAKSWQEEEEEEEEEEEEEEEAAEEDEKEVKVKKSLKWLMEEENRERLVMEMGPD 300
           RCRSAPA+ W EEE EE ++E+E          +VKVKKSLKWLMEEENRERLV+EMG D
Sbjct: 248 RCRSAPARRWMEEESEEGDDEKE----------KVKVKKSLKWLMEEENRERLVVEMGTD 307

Query: 301 FCRMSSEIAKETWVGRDLFSRSRSWK 317
           FCRM+S+ AKE       F+RS+SWK
Sbjct: 308 FCRMTSDNAKE-------FTRSQSWK 308

BLAST of MS008269 vs. ExPASy TrEMBL
Match: A0A2I4DXY3 (uncharacterized protein LOC108984494 OS=Juglans regia OX=51240 GN=LOC108984494 PE=4 SV=1)

HSP 1 Score: 358.2 bits (918), Expect = 3.5e-95
Identity = 216/341 (63.34%), Postives = 254/341 (74.49%), Query Frame = 0

Query: 4   GRDAKAIHSADLLVCFPSRSNLTLMPKPLCSPARGLDSNKLRRSHRHHHR---RRKST-- 63
           GR+AKA  SADLLVCFPSR++LTLMPKP+CSPAR  + NK   SH HHH    ++ ST  
Sbjct: 3   GREAKAAPSADLLVCFPSRAHLTLMPKPICSPARHSEPNKRHHSHHHHHHHHLKKASTRG 62

Query: 64  -SAASPLIWAKPKTMGSEISEPSSPKVTCAGQIKIRPKTGSCKSWQSVMEEIERIHNRRK 123
              ASPL+WAK K MGSEISEP+SPKVTCAGQIK+RPKT SCKSWQSVMEEIERIHN RK
Sbjct: 63  GGQASPLLWAKTKPMGSEISEPTSPKVTCAGQIKVRPKTSSCKSWQSVMEEIERIHNNRK 122

Query: 124 LRRRRSNWVESLGFKKDIMQFLTCLRNIRFDFRCFKAFPEADFTTEEEDEEEEEEEEEGK 183
             ++R +WVE+LG KKDIMQFLTCLR+IRFDFRCF +FPE+D TTE+ED    +EEE G+
Sbjct: 123 -HKKRPSWVEALGLKKDIMQFLTCLRSIRFDFRCFGSFPESDITTEDED----DEEEIGE 182

Query: 184 SQENQVGVE---GNESSRTAFSKWFMVLQESGASNGICR------------ESNGPPLAP 243
            Q+N +G E   GNE+SRTAFSKWFMVLQE+  +N +C+            ES     AP
Sbjct: 183 CQDNHLGTERSDGNETSRTAFSKWFMVLQEN-QNNKLCKQEKRETKKTYDDESIAASAAP 242

Query: 244 PKNALLLMRCRSAPAKSWQEEEEEEEEEEEEEEEAAEEDEKEVKVKKSLKWLMEEENRER 303
           P NALLLMRCRSAPAK W  E+E+EEEE EE+E+  E   KEV+  KSLK LMEEE R +
Sbjct: 243 PPNALLLMRCRSAPAKGWLVEKEDEEEEGEEDED-EEGTGKEVRKAKSLKCLMEEEKRTK 302

Query: 304 ----LVMEMGPDFCRMSSEIAKETWVG---RDLFSRSRSWK 317
               +VM+   DF ++SS+IAKETWVG   RD FSRSRSWK
Sbjct: 303 KENMVVMKYDTDFYKISSDIAKETWVGGGMRDPFSRSRSWK 336

BLAST of MS008269 vs. TAIR 10
Match: AT1G78110.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: plasma membrane; EXPRESSED IN: 17 plant structures; EXPRESSED DURING: 7 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G22230.1); Has 5452 Blast hits to 3541 proteins in 289 species: Archae - 4; Bacteria - 165; Metazoa - 1756; Fungi - 532; Plants - 205; Viruses - 141; Other Eukaryotes - 2649 (source: NCBI BLink). )

HSP 1 Score: 255.4 bits (651), Expect = 6.1e-68
Identity = 174/340 (51.18%), Postives = 220/340 (64.71%), Query Frame = 0

Query: 11  HSADLLVCFPSRSNLTLMPKPLCSPARGLDSNKLRRSHRHHHRRRKSTSA-------ASP 70
           +SADLLVCFPSR++L L PKP+CSP+R  DS+  RR    HHRR+ S  +        SP
Sbjct: 16  YSADLLVCFPSRTHLALTPKPICSPSRPSDSSTNRRP---HHRRQLSKLSGGGGGGHGSP 75

Query: 71  LIWAK---PKTM-GSEISEPSSPKVTCAGQIKIRPKT--GSCKSWQSVMEEIERIHNRRK 130
           ++WAK    K M G EI+EP+SPKVTCAGQIK+RP    G  K+WQSVMEEIERIH+ R 
Sbjct: 76  VLWAKQASSKNMGGDEIAEPTSPKVTCAGQIKVRPSKCGGRGKNWQSVMEEIERIHDNRS 135

Query: 131 LRRRRSNWVESLGFKKDIMQFLTCLRNIRFDFRCFKAFPEADFTTEEEDEEEEEEEEEGK 190
             +         G KKD+M FLTCLRNI+FDFRCF  F  AD T+++++EE+++++EE  
Sbjct: 136 QSK-------FFGLKKDVMGFLTCLRNIKFDFRCFGDFRHADVTSDDDEEEDDDDDEE-- 195

Query: 191 SQENQVGVEGNESSRTAFSKWFMVLQE------SGASNGICRE------SNGPPLAPPKN 250
             E  V  E  E+S+T FSKWFMVLQE         +N  C E      +   P  PP N
Sbjct: 196 --EEVVEGEEEENSKTVFSKWFMVLQEEQNNKDDDKNNNKCDEKRDLEDTETEPAVPPPN 255

Query: 251 ALLLMRCRSAPAKSWQEEE-----EEEEEEEEEEEEAAEEDEKEVKV-KKSLKWLMEEEN 310
           ALLLMRCRSAPAKSW EE      E+E+ EE++EE+  E+ E  +K  KK L+ LMEEE 
Sbjct: 256 ALLLMRCRSAPAKSWLEERMKVKTEQEKREEQKEEKETEDQETSMKTKKKDLRSLMEEEK 315

Query: 311 RERLVMEMGPDFCRMSSEIAKETWVG---RDLFSRSRSWK 317
            E ++M    +F R+SS+IAKETWV    +D  SRSRSWK
Sbjct: 316 MELVLMRYDTEFYRLSSDIAKETWVVGGIQDPLSRSRSWK 341

BLAST of MS008269 vs. TAIR 10
Match: AT1G22230.1 (unknown protein; LOCATED IN: chloroplast; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G78110.1); Has 2358 Blast hits to 1759 proteins in 159 species: Archae - 2; Bacteria - 36; Metazoa - 1046; Fungi - 203; Plants - 157; Viruses - 72; Other Eukaryotes - 842 (source: NCBI BLink). )

HSP 1 Score: 198.7 bits (504), Expect = 6.8e-51
Identity = 150/333 (45.05%), Postives = 206/333 (61.86%), Query Frame = 0

Query: 1   MKLGRDAKAIHSADLLVCFPSRSNLTLMPKPLCSPARGLDSNKLRRSHRHHHRRRKSTSA 60
           +K G    + +SADL+VCFPSR++L+L  K + SP+   +    RR +  HHRR  S  +
Sbjct: 2   IKGGGGKSSGYSADLMVCFPSRAHLSLPSKSISSPSSSFN----RRQNAPHHRRSISKLS 61

Query: 61  ASPLIWAKPKTMGSE-ISEPSSPKVTCAGQIKIR--PKTGSCKSWQSVMEEIERIHNRRK 120
           +S     + +  G E + EP+SPKVTCAGQIK+R   + G  K+WQS+M EIE+IH    
Sbjct: 62  SSGGGVRQNRGGGREVVEEPTSPKVTCAGQIKVRSSKRDGGGKNWQSLMAEIEKIH---- 121

Query: 121 LRRRRSNWVESLGFKKDIMQFLTCLRNIRFDFRCFKAFPEADFTTEEEDEEEEEEEEEGK 180
              R  +  +  G K+D+M FLTCLR+  FDFRCF AFP  D  +++E+E+EEEEEE+ +
Sbjct: 122 ---RSKSESKFFGIKRDVMGFLTCLRD--FDFRCFGAFPPVDIISDDEEEDEEEEEEDEE 181

Query: 181 SQENQVGVEGNESSRTAFSKWFMVLQESGASNGICRE------SNGPPLAPPKNALLLMR 240
             E       +ESS T FSKW MVL E   +N  C +      S+     PP NALLLMR
Sbjct: 182 EDE-------DESSGTVFSKWLMVLHEK-QNNEECVDGKENVFSDVETAVPPPNALLLMR 241

Query: 241 CRSAPAKSWQEEEEEEEEE-EEEEEEAAEEDEKE---VKVKKSLKWLMEEENRERL-VME 300
           CRSAP K+W EE++EE EE +   +++ EE+E+E   V  KK L+ LMEEE +  L VM 
Sbjct: 242 CRSAPVKNWSEEKKEETEEGDNRVKQSGEEEEEEKDRVGNKKDLRSLMEEEKKMNLVVMN 301

Query: 301 MGPDFCRMSSEIAKETWVG---RDLFSRSRSWK 317
              ++ ++S++IAKETWV    +D   RSRSWK
Sbjct: 302 YDTNYYKLSNDIAKETWVVGGIQDPLFRSRSWK 313

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022147766.17.8e-16698.14uncharacterized protein LOC111016595 [Momordica charantia][more]
XP_038898663.17.1e-11975.45transcription initiation factor IIE subunit alpha [Benincasa hispida][more]
XP_004142611.22.0e-10870.64transcription initiation factor IIE subunit alpha [Cucumis sativus] >KAE8649637.... [more]
XP_008444111.11.1e-10670.86PREDICTED: uncharacterized protein LOC103487551 [Cucumis melo] >KAA0064246.1 tra... [more]
XP_023523446.11.9e-9566.35uncharacterized protein LOC111787660 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A6J1D3C23.8e-16698.14uncharacterized protein LOC111016595 OS=Momordica charantia OX=3673 GN=LOC111016... [more]
A0A0A0L1Z49.4e-10970.64Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G377750 PE=4 SV=1[more]
A0A5D3D5035.2e-10770.86Transcription initiation factor IIE subunit alpha-like OS=Cucumis melo var. maku... [more]
A0A1S3B9495.2e-10770.86uncharacterized protein LOC103487551 OS=Cucumis melo OX=3656 GN=LOC103487551 PE=... [more]
A0A2I4DXY33.5e-9563.34uncharacterized protein LOC108984494 OS=Juglans regia OX=51240 GN=LOC108984494 P... [more]
Match NameE-valueIdentityDescription
AT1G78110.16.1e-6851.18unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic... [more]
AT1G22230.16.8e-5145.05unknown protein; LOCATED IN: chloroplast; BEST Arabidopsis thaliana protein matc... [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 237..268
NoneNo IPR availableCOILSCoilCoilcoord: 162..182
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 36..62
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 160..177
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 160..189
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 238..266
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 241..260
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 43..58
NoneNo IPR availablePANTHERPTHR33448:SF3OS09G0370000 PROTEINcoord: 4..316
NoneNo IPR availablePANTHERPTHR33448CHLOROPLAST PROTEIN HCF243-RELATEDcoord: 4..316

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS008269.1MS008269.1mRNA