MS008260 (gene) Bitter gourd (TR) v1

Overview


Name	MS008260
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Cytochrome P450
Location	scaffold4: 13100 .. 13561 (-)
RNA-Seq Expression	MS008260
Synteny	MS008260

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

GATTTGGAAAGGCTTCCGTATCTGCAGTGCGTTATAAAGGAGACGCTGCGGATGTACCCGGTGGGGCCCTTACTGATGCCCCACGAATCGTCGGAGGATTGTACTGTGGGTGGGTACCACATCCCACGTGGCACCATGTTACTGGTGAACGCGTGGGCGATCCACAACGACGGCGGAAACTGGGAGGAGCCGGCGGCGTTCAGGCCGGATAGATTCTTGGAGGCGGCAGCGGGCGGCGGAGATGGGTTGAGATATGTGCCGTTTGGGGCGGGGAGGAGGGGGTGCCCCGGGGAGGCGTTGGCCGTGAAAGTGGTGGGGCTGGTAGTGGGGTCGCTGATTCAGTGCTTTGAGTGGGACAGAATTGGAGAGGAAATGGTGGACATGGCCGAGGGAACAGGCCTCACCATGTCCAAGGCCCATCCTTTGCAAGCAAAATTCAGGCCCAGCCCAATTCTCCGCCGC

mRNA sequence

GATTTGGAAAGGCTTCCGTATCTGCAGTGCGTTATAAAGGAGACGCTGCGGATGTACCCGGTGGGGCCCTTACTGATGCCCCACGAATCGTCGGAGGATTGTACTGTGGGTGGGTACCACATCCCACGTGGCACCATGTTACTGGTGAACGCGTGGGCGATCCACAACGACGGCGGAAACTGGGAGGAGCCGGCGGCGTTCAGGCCGGATAGATTCTTGGAGGCGGCAGCGGGCGGCGGAGATGGGTTGAGATATGTGCCGTTTGGGGCGGGGAGGAGGGGGTGCCCCGGGGAGGCGTTGGCCGTGAAAGTGGTGGGGCTGGTAGTGGGGTCGCTGATTCAGTGCTTTGAGTGGGACAGAATTGGAGAGGAAATGGTGGACATGGCCGAGGGAACAGGCCTCACCATGTCCAAGGCCCATCCTTTGCAAGCAAAATTCAGGCCCAGCCCAATTCTCCGCCGC

Coding sequence (CDS)

GATTTGGAAAGGCTTCCGTATCTGCAGTGCGTTATAAAGGAGACGCTGCGGATGTACCCGGTGGGGCCCTTACTGATGCCCCACGAATCGTCGGAGGATTGTACTGTGGGTGGGTACCACATCCCACGTGGCACCATGTTACTGGTGAACGCGTGGGCGATCCACAACGACGGCGGAAACTGGGAGGAGCCGGCGGCGTTCAGGCCGGATAGATTCTTGGAGGCGGCAGCGGGCGGCGGAGATGGGTTGAGATATGTGCCGTTTGGGGCGGGGAGGAGGGGGTGCCCCGGGGAGGCGTTGGCCGTGAAAGTGGTGGGGCTGGTAGTGGGGTCGCTGATTCAGTGCTTTGAGTGGGACAGAATTGGAGAGGAAATGGTGGACATGGCCGAGGGAACAGGCCTCACCATGTCCAAGGCCCATCCTTTGCAAGCAAAATTCAGGCCCAGCCCAATTCTCCGCCGC

Protein sequence

DLERLPYLQCVIKETLRMYPVGPLLMPHESSEDCTVGGYHIPRGTMLLVNAWAIHNDGGNWEEPAAFRPDRFLEAAAGGGDGLRYVPFGAGRRGCPGEALAVKVVGLVVGSLIQCFEWDRIGEEMVDMAEGTGLTMSKAHPLQAKFRPSPILRR

Homology

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BLAST of MS008260 vs. NCBI nr
Match: XP_022140041.1 (cytochrome P450 81D1-like [Momordica charantia])

HSP 1 Score: 303.1 bits (775), Expect = 1.3e-78
Identity = 141/154 (91.56%), Postives = 145/154 (94.16%), Query Frame = 0


Query: 1   DLERLPYLQCVIKETLRMYPVGPLLMPHESSEDCTVGGYHIPRGTMLLVNAWAIHNDGGN 60
           DLE+LPYLQCVIKETLRM+PVGPLL+PHESS DCTVGGYHIPRGTMLLVNAWAIHNDGGN
Sbjct: 352 DLEKLPYLQCVIKETLRMHPVGPLLVPHESSADCTVGGYHIPRGTMLLVNAWAIHNDGGN 411

Query: 61  WEEPAAFRPDRFLEAAAGGGDGLRYVPFGAGRRGCPGEALAVKVVGLVVGSLIQCFEWDR 120
           WEEPAAFRP+RFLE AAG GDGLRYVPFG GRRGCPGE LAVKVVGLVVGSLIQCFEWDR
Sbjct: 412 WEEPAAFRPERFLEVAAGAGDGLRYVPFGFGRRGCPGETLAVKVVGLVVGSLIQCFEWDR 471

Query: 121 IGEEMVDMAEGTGLTMSKAHPLQAKFRPSPILRR 155
           IGEEMVDMAEGTGLTM KAHPLQAK RP PIL R
Sbjct: 472 IGEEMVDMAEGTGLTMPKAHPLQAKCRPRPILYR 505

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BLAST of MS008260 vs. NCBI nr
Match: XP_022142908.1 (cytochrome P450 81D11-like [Momordica charantia])

HSP 1 Score: 297.7 bits (761), Expect = 5.6e-77
Identity = 140/154 (90.91%), Postives = 143/154 (92.86%), Query Frame = 0


Query: 1   DLERLPYLQCVIKETLRMYPVGPLLMPHESSEDCTVGGYHIPRGTMLLVNAWAIHNDGGN 60
           DLE+L YLQCVIKETLRMYPVGPLL+PHESS DCTVGGYHIPRGTMLLVN WAIHNDG N
Sbjct: 383 DLEKLRYLQCVIKETLRMYPVGPLLVPHESSADCTVGGYHIPRGTMLLVNVWAIHNDGRN 442

Query: 61  WEEPAAFRPDRFLEAAAGGGDGLRYVPFGAGRRGCPGEALAVKVVGLVVGSLIQCFEWDR 120
           WEEPAAFRP+RFLEAAAGGGDGLRYVPFG GRRGCPGE LAVKVVGLVVGSLIQCFEWDR
Sbjct: 443 WEEPAAFRPERFLEAAAGGGDGLRYVPFGFGRRGCPGETLAVKVVGLVVGSLIQCFEWDR 502

Query: 121 IGEEMVDMAEGTGLTMSKAHPLQAKFRPSPILRR 155
           IGEEMVDMAE TGLTM KAHPLQAK RP PIL R
Sbjct: 503 IGEEMVDMAERTGLTMPKAHPLQAKCRPRPILYR 536

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BLAST of MS008260 vs. NCBI nr
Match: XP_008444122.1 (PREDICTED: cytochrome P450 81D1-like [Cucumis melo] >TYK19213.1 cytochrome P450 81D1-like [Cucumis melo var. makuwa])

HSP 1 Score: 263.8 bits (673), Expect = 9.0e-67
Identity = 123/154 (79.87%), Postives = 135/154 (87.66%), Query Frame = 0


Query: 1   DLERLPYLQCVIKETLRMYPVGPLLMPHESSEDCTVGGYHIPRGTMLLVNAWAIHNDGGN 60
           DLE+LPYLQCVIKET+RMYP GPLL+PHESS DCTVGGYHIPRGTML+VNAWAIHND G 
Sbjct: 349 DLEKLPYLQCVIKETMRMYPAGPLLVPHESSADCTVGGYHIPRGTMLMVNAWAIHNDAGL 408

Query: 61  WEEPAAFRPDRFLEAAA--GGGDGLRYVPFGAGRRGCPGEALAVKVVGLVVGSLIQCFEW 120
           WEE A F+P+RFL AAA  G G GL+Y+ FGAGRRGCPGE LA+KVVGLV+GSLIQCFEW
Sbjct: 409 WEEAAVFKPERFLAAAAAEGDGTGLKYMVFGAGRRGCPGEGLAMKVVGLVLGSLIQCFEW 468

Query: 121 DRIGEEMVDMAEGTGLTMSKAHPLQAKFRPSPIL 153
           +RIGEEMVDM EGTGL+M KA PLQAK  P PIL
Sbjct: 469 ERIGEEMVDMGEGTGLSMPKACPLQAKCSPRPIL 502

[Close]

BLAST of MS008260 vs. NCBI nr
Match: KAA0052386.1 (cytochrome P450 81D1-like [Cucumis melo var. makuwa])

HSP 1 Score: 263.8 bits (673), Expect = 9.0e-67
Identity = 123/154 (79.87%), Postives = 135/154 (87.66%), Query Frame = 0


Query: 1   DLERLPYLQCVIKETLRMYPVGPLLMPHESSEDCTVGGYHIPRGTMLLVNAWAIHNDGGN 60
           DLE+LPYLQCVIKET+RMYP GPLL+PHESS DCTVGGYHIPRGTML+VNAWAIHND G 
Sbjct: 385 DLEKLPYLQCVIKETMRMYPAGPLLVPHESSADCTVGGYHIPRGTMLMVNAWAIHNDAGL 444

Query: 61  WEEPAAFRPDRFLEAAA--GGGDGLRYVPFGAGRRGCPGEALAVKVVGLVVGSLIQCFEW 120
           WEE A F+P+RFL AAA  G G GL+Y+ FGAGRRGCPGE LA+KVVGLV+GSLIQCFEW
Sbjct: 445 WEEAAVFKPERFLAAAAAEGDGTGLKYMVFGAGRRGCPGEGLAMKVVGLVLGSLIQCFEW 504

Query: 121 DRIGEEMVDMAEGTGLTMSKAHPLQAKFRPSPIL 153
           +RIGEEMVDM EGTGL+M KA PLQAK  P PIL
Sbjct: 505 ERIGEEMVDMGEGTGLSMPKACPLQAKCSPRPIL 538

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BLAST of MS008260 vs. NCBI nr
Match: XP_004142688.1 (cytochrome P450 81Q32 [Cucumis sativus] >KGN54600.1 hypothetical protein Csa_012553 [Cucumis sativus])

HSP 1 Score: 263.1 bits (671), Expect = 1.5e-66
Identity = 123/153 (80.39%), Postives = 135/153 (88.24%), Query Frame = 0


Query: 1   DLERLPYLQCVIKETLRMYPVGPLLMPHESSEDCTVGGYHIPRGTMLLVNAWAIHNDGGN 60
           DLE+LPYLQCVIKET+RMYPVGPLL+PHESS DCTVGGYHIP GTML+VNAWAIHND G 
Sbjct: 350 DLEKLPYLQCVIKETMRMYPVGPLLVPHESSADCTVGGYHIPGGTMLMVNAWAIHNDAGL 409

Query: 61  WEEPAAFRPDRFLEAAA-GGGDGLRYVPFGAGRRGCPGEALAVKVVGLVVGSLIQCFEWD 120
           WEE A F+P+RFL A A G G GL+Y+ FGAGRRGCPGE LA++VVGLV+GSLIQCFEW+
Sbjct: 410 WEEAAVFKPERFLGAGAEGDGIGLKYMVFGAGRRGCPGEGLAMRVVGLVLGSLIQCFEWE 469

Query: 121 RIGEEMVDMAEGTGLTMSKAHPLQAKFRPSPIL 153
           RIGEEMVDM EGTGLTM KA PLQAK RP PIL
Sbjct: 470 RIGEEMVDMGEGTGLTMPKACPLQAKCRPRPIL 502

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BLAST of MS008260 vs. ExPASy Swiss-Prot
Match: W8JMU7 (Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1)

HSP 1 Score: 186.0 bits (471), Expect = 3.1e-46
Identity = 88/157 (56.05%), Postives = 110/157 (70.06%), Query Frame = 0


Query: 1   DLERLPYLQCVIKETLRMYPVGPLLMPHESSEDCTVGGYHIPRGTMLLVNAWAIHNDGGN 60
           DL +L YL  +I ET R+ P  P+L+PHESS+DC V GY +P+GT+LLVNAWAIH D   
Sbjct: 343 DLPKLTYLHNIISETFRLCPAAPMLVPHESSDDCKVQGYDVPKGTILLVNAWAIHRDPEF 402

Query: 61  WEEPAAFRPDRFLEAAAGGGDGL---RYVPFGAGRRGCPGEALAVKVVGLVVGSLIQCFE 120
           W+EP  F+P+R       GG  L   + +PFG GRR CPG  LA +VVGL +G+LIQCFE
Sbjct: 403 WDEPTLFKPERH------GGVELEPSKLMPFGMGRRSCPGSGLAQRVVGLTLGALIQCFE 462

Query: 121 WDRIGEEMVDMAEGTGLTMSKAHPLQAKFRPSPILRR 155
           W RIGE  +DMAEG+GLTM KA PL+A  +P  IL +
Sbjct: 463 WKRIGEAKIDMAEGSGLTMPKAQPLEALCKPRNILHK 493

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BLAST of MS008260 vs. ExPASy Swiss-Prot
Match: Q9LHA1 (Cytochrome P450 81D11 OS=Arabidopsis thaliana OX=3702 GN=CYP81D11 PE=2 SV=1)

HSP 1 Score: 181.0 bits (458), Expect = 1.0e-44
Identity = 82/155 (52.90%), Postives = 110/155 (70.97%), Query Frame = 0


Query: 1   DLERLPYLQCVIKETLRMYPVGPLLMPHESSEDCTVGGYHIPRGTMLLVNAWAIHNDGGN 60
           D+ +LPYLQ ++ ETLR+YPV P+L+PH +SEDC V GY +PRGT++LVNAWAIH D   
Sbjct: 352 DIVKLPYLQHIVSETLRLYPVAPMLLPHLASEDCIVDGYDVPRGTIILVNAWAIHRDPKL 411

Query: 61  WEEPAAFRPDRFLEAAAGGGDGLRYVPFGAGRRGCPGEALAVKVVGLVVGSLIQCFEWDR 120
           WEEP  F+P+RF +     G+  + +PFG GRR CPG  LA ++V L +GSL+QCFEW+R
Sbjct: 412 WEEPEKFKPERFEKK----GEDKKLMPFGIGRRSCPGSGLAQRLVTLALGSLVQCFEWER 471

Query: 121 IGEEMVDMAEG-TGLTMSKAHPLQAKFRPSPILRR 155
           + E+ +DM E   G TM KA  LQA  +  PI+ +
Sbjct: 472 VEEKYLDMRESEKGTTMRKATSLQAMCKARPIVHK 502

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BLAST of MS008260 vs. ExPASy Swiss-Prot
Match: Q9FG65 (Cytochrome P450 81D1 OS=Arabidopsis thaliana OX=3702 GN=CYP81D1 PE=2 SV=1)

HSP 1 Score: 180.3 bits (456), Expect = 1.7e-44
Identity = 80/154 (51.95%), Postives = 107/154 (69.48%), Query Frame = 0


Query: 1   DLERLPYLQCVIKETLRMYPVGPLLMPHESSEDCTVGGYHIPRGTMLLVNAWAIHNDGGN 60
           DL  LPYL+ ++ ETLR++P  PLL+PH +SEDC +G Y +PRGT LLVNAWAIH D   
Sbjct: 350 DLSELPYLKNIVLETLRLHPATPLLVPHMASEDCKIGSYDMPRGTTLLVNAWAIHRDPNT 409

Query: 61  WEEPAAFRPDRFLEAAAGGGDGLRYVPFGAGRRGCPGEALAVKVVGLVVGSLIQCFEWDR 120
           W++P +F+P+RF +      +  + + FG GRR CPG  LA ++VGL +GSLIQCFEW+R
Sbjct: 410 WDDPDSFKPERFEKEE----EAQKLLAFGLGRRACPGSGLAQRIVGLALGSLIQCFEWER 469

Query: 121 IGEEMVDMAEGTGLTMSKAHPLQAKFRPSPILRR 155
           +G   VDM EG G T+ KA PL+A  +  P L +
Sbjct: 470 VGNVEVDMKEGVGNTVPKAIPLKAICKARPFLHK 499

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BLAST of MS008260 vs. ExPASy Swiss-Prot
Match: Q0WTF4 (Cytochrome P450 81F3 OS=Arabidopsis thaliana OX=3702 GN=CYP81F3 PE=2 SV=1)

HSP 1 Score: 170.6 bits (431), Expect = 1.4e-41
Identity = 78/157 (49.68%), Postives = 101/157 (64.33%), Query Frame = 0


Query: 1   DLERLPYLQCVIKETLRMYPVGPLLMPHESSEDCTVGGYHIPRGTMLLVNAWAIHNDGGN 60
           D+  LPYLQ ++ ET R+YP  PLL+P   +ED  VGGY +PRGTM++VNAWAIH D   
Sbjct: 339 DIANLPYLQNIVSETFRLYPAAPLLVPRSPTEDIKVGGYDVPRGTMVMVNAWAIHRDPEL 398

Query: 61  WEEPAAFRPDRFLEAAAGGGDG---LRYVPFGAGRRGCPGEALAVKVVGLVVGSLIQCFE 120
           W EP  F+P+RF     GGG G    + +PFG GRR CPG  L  K+V L +GSLIQCF+
Sbjct: 399 WNEPEKFKPERF-NGGEGGGRGEDVHKLMPFGNGRRSCPGAGLGQKIVTLALGSLIQCFD 458

Query: 121 WDRIGEEMVDMAEGTGLTMSKAHPLQAKFRPSPILRR 155
           W ++  E +DM E  G+ M K  PL A  +  PI+ +
Sbjct: 459 WQKVNGEAIDMTETPGMAMRKKIPLSALCQSRPIMSK 494

[Close]

BLAST of MS008260 vs. ExPASy Swiss-Prot
Match: O65790 (Cytochrome P450 81F1 OS=Arabidopsis thaliana OX=3702 GN=CYP81F1 PE=2 SV=2)

HSP 1 Score: 169.9 bits (429), Expect = 2.3e-41
Identity = 75/154 (48.70%), Postives = 101/154 (65.58%), Query Frame = 0


Query: 1   DLERLPYLQCVIKETLRMYPVGPLLMPHESSEDCTVGGYHIPRGTMLLVNAWAIHNDGGN 60
           D+  LPYLQ V+ ET R++PV P L+P   ++D  +GGY +PR T+++VNAWAIH D   
Sbjct: 344 DIAVLPYLQNVVSETFRLFPVAPFLIPRSPTDDMKIGGYDVPRDTIVMVNAWAIHRDPEI 403

Query: 61  WEEPAAFRPDRFLEAAAGGGDGLRYVPFGAGRRGCPGEALAVKVVGLVVGSLIQCFEWDR 120
           WEEP  F PDR+ +         + +PFG GRR CPG  L  ++V L +GSLIQCFEW+ 
Sbjct: 404 WEEPEKFNPDRYNDGCGSDYYVYKLMPFGNGRRTCPGAGLGQRIVTLALGSLIQCFEWEN 463

Query: 121 IGEEMVDMAEGTGLTMSKAHPLQAKFRPSPILRR 155
           +  E +DM+E TGL M K  PL+A  RP PI+ +
Sbjct: 464 VKGEEMDMSESTGLGMRKMDPLRAMCRPRPIMSK 497

[Close]

BLAST of MS008260 vs. ExPASy TrEMBL
Match: A0A6J1CEJ7 (cytochrome P450 81D1-like OS=Momordica charantia OX=3673 GN=LOC111010788 PE=3 SV=1)

HSP 1 Score: 303.1 bits (775), Expect = 6.5e-79
Identity = 141/154 (91.56%), Postives = 145/154 (94.16%), Query Frame = 0


Query: 1   DLERLPYLQCVIKETLRMYPVGPLLMPHESSEDCTVGGYHIPRGTMLLVNAWAIHNDGGN 60
           DLE+LPYLQCVIKETLRM+PVGPLL+PHESS DCTVGGYHIPRGTMLLVNAWAIHNDGGN
Sbjct: 352 DLEKLPYLQCVIKETLRMHPVGPLLVPHESSADCTVGGYHIPRGTMLLVNAWAIHNDGGN 411

Query: 61  WEEPAAFRPDRFLEAAAGGGDGLRYVPFGAGRRGCPGEALAVKVVGLVVGSLIQCFEWDR 120
           WEEPAAFRP+RFLE AAG GDGLRYVPFG GRRGCPGE LAVKVVGLVVGSLIQCFEWDR
Sbjct: 412 WEEPAAFRPERFLEVAAGAGDGLRYVPFGFGRRGCPGETLAVKVVGLVVGSLIQCFEWDR 471

Query: 121 IGEEMVDMAEGTGLTMSKAHPLQAKFRPSPILRR 155
           IGEEMVDMAEGTGLTM KAHPLQAK RP PIL R
Sbjct: 472 IGEEMVDMAEGTGLTMPKAHPLQAKCRPRPILYR 505

[Close]

BLAST of MS008260 vs. ExPASy TrEMBL
Match: A0A6J1CPF5 (cytochrome P450 81D11-like OS=Momordica charantia OX=3673 GN=LOC111012906 PE=3 SV=1)

HSP 1 Score: 297.7 bits (761), Expect = 2.7e-77
Identity = 140/154 (90.91%), Postives = 143/154 (92.86%), Query Frame = 0


Query: 1   DLERLPYLQCVIKETLRMYPVGPLLMPHESSEDCTVGGYHIPRGTMLLVNAWAIHNDGGN 60
           DLE+L YLQCVIKETLRMYPVGPLL+PHESS DCTVGGYHIPRGTMLLVN WAIHNDG N
Sbjct: 383 DLEKLRYLQCVIKETLRMYPVGPLLVPHESSADCTVGGYHIPRGTMLLVNVWAIHNDGRN 442

Query: 61  WEEPAAFRPDRFLEAAAGGGDGLRYVPFGAGRRGCPGEALAVKVVGLVVGSLIQCFEWDR 120
           WEEPAAFRP+RFLEAAAGGGDGLRYVPFG GRRGCPGE LAVKVVGLVVGSLIQCFEWDR
Sbjct: 443 WEEPAAFRPERFLEAAAGGGDGLRYVPFGFGRRGCPGETLAVKVVGLVVGSLIQCFEWDR 502

Query: 121 IGEEMVDMAEGTGLTMSKAHPLQAKFRPSPILRR 155
           IGEEMVDMAE TGLTM KAHPLQAK RP PIL R
Sbjct: 503 IGEEMVDMAERTGLTMPKAHPLQAKCRPRPILYR 536

[Close]

BLAST of MS008260 vs. ExPASy TrEMBL
Match: A0A5A7UDX9 (Cytochrome P450 81D1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold1994G00040 PE=3 SV=1)

HSP 1 Score: 263.8 bits (673), Expect = 4.3e-67
Identity = 123/154 (79.87%), Postives = 135/154 (87.66%), Query Frame = 0


Query: 1   DLERLPYLQCVIKETLRMYPVGPLLMPHESSEDCTVGGYHIPRGTMLLVNAWAIHNDGGN 60
           DLE+LPYLQCVIKET+RMYP GPLL+PHESS DCTVGGYHIPRGTML+VNAWAIHND G 
Sbjct: 385 DLEKLPYLQCVIKETMRMYPAGPLLVPHESSADCTVGGYHIPRGTMLMVNAWAIHNDAGL 444

Query: 61  WEEPAAFRPDRFLEAAA--GGGDGLRYVPFGAGRRGCPGEALAVKVVGLVVGSLIQCFEW 120
           WEE A F+P+RFL AAA  G G GL+Y+ FGAGRRGCPGE LA+KVVGLV+GSLIQCFEW
Sbjct: 445 WEEAAVFKPERFLAAAAAEGDGTGLKYMVFGAGRRGCPGEGLAMKVVGLVLGSLIQCFEW 504

Query: 121 DRIGEEMVDMAEGTGLTMSKAHPLQAKFRPSPIL 153
           +RIGEEMVDM EGTGL+M KA PLQAK  P PIL
Sbjct: 505 ERIGEEMVDMGEGTGLSMPKACPLQAKCSPRPIL 538

[Close]

BLAST of MS008260 vs. ExPASy TrEMBL
Match: A0A5D3D6T6 (Cytochrome P450 81D1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1493G00050 PE=3 SV=1)

HSP 1 Score: 263.8 bits (673), Expect = 4.3e-67
Identity = 123/154 (79.87%), Postives = 135/154 (87.66%), Query Frame = 0


Query: 1   DLERLPYLQCVIKETLRMYPVGPLLMPHESSEDCTVGGYHIPRGTMLLVNAWAIHNDGGN 60
           DLE+LPYLQCVIKET+RMYP GPLL+PHESS DCTVGGYHIPRGTML+VNAWAIHND G 
Sbjct: 349 DLEKLPYLQCVIKETMRMYPAGPLLVPHESSADCTVGGYHIPRGTMLMVNAWAIHNDAGL 408

Query: 61  WEEPAAFRPDRFLEAAA--GGGDGLRYVPFGAGRRGCPGEALAVKVVGLVVGSLIQCFEW 120
           WEE A F+P+RFL AAA  G G GL+Y+ FGAGRRGCPGE LA+KVVGLV+GSLIQCFEW
Sbjct: 409 WEEAAVFKPERFLAAAAAEGDGTGLKYMVFGAGRRGCPGEGLAMKVVGLVLGSLIQCFEW 468

Query: 121 DRIGEEMVDMAEGTGLTMSKAHPLQAKFRPSPIL 153
           +RIGEEMVDM EGTGL+M KA PLQAK  P PIL
Sbjct: 469 ERIGEEMVDMGEGTGLSMPKACPLQAKCSPRPIL 502

[Close]

BLAST of MS008260 vs. ExPASy TrEMBL
Match: A0A1S3B958 (cytochrome P450 81D1-like OS=Cucumis melo OX=3656 GN=LOC103487563 PE=3 SV=1)

HSP 1 Score: 263.8 bits (673), Expect = 4.3e-67
Identity = 123/154 (79.87%), Postives = 135/154 (87.66%), Query Frame = 0


Query: 1   DLERLPYLQCVIKETLRMYPVGPLLMPHESSEDCTVGGYHIPRGTMLLVNAWAIHNDGGN 60
           DLE+LPYLQCVIKET+RMYP GPLL+PHESS DCTVGGYHIPRGTML+VNAWAIHND G 
Sbjct: 349 DLEKLPYLQCVIKETMRMYPAGPLLVPHESSADCTVGGYHIPRGTMLMVNAWAIHNDAGL 408

Query: 61  WEEPAAFRPDRFLEAAA--GGGDGLRYVPFGAGRRGCPGEALAVKVVGLVVGSLIQCFEW 120
           WEE A F+P+RFL AAA  G G GL+Y+ FGAGRRGCPGE LA+KVVGLV+GSLIQCFEW
Sbjct: 409 WEEAAVFKPERFLAAAAAEGDGTGLKYMVFGAGRRGCPGEGLAMKVVGLVLGSLIQCFEW 468

Query: 121 DRIGEEMVDMAEGTGLTMSKAHPLQAKFRPSPIL 153
           +RIGEEMVDM EGTGL+M KA PLQAK  P PIL
Sbjct: 469 ERIGEEMVDMGEGTGLSMPKACPLQAKCSPRPIL 502

[Close]

BLAST of MS008260 vs. TAIR 10
Match: AT4G37360.1 (cytochrome P450, family 81, subfamily D, polypeptide 2 )

HSP 1 Score: 193.4 bits (490), Expect = 1.4e-49
Identity = 86/147 (58.50%), Postives = 109/147 (74.15%), Query Frame = 0


Query: 1   DLERLPYLQCVIKETLRMYPVGPLLMPHESSEDCTVGGYHIPRGTMLLVNAWAIHNDGGN 60
           D+  LPYLQ ++ ETLR+YP  P+L+PH +S+DC VGGY +PRGTMLL NAWAIH D   
Sbjct: 344 DIPNLPYLQNIVSETLRLYPAAPMLLPHVASKDCKVGGYDMPRGTMLLTNAWAIHRDPLL 403

Query: 61  WEEPAAFRPDRFLEAAAGGGDGLRYVPFGAGRRGCPGEALAVKVVGLVVGSLIQCFEWDR 120
           W++P +F+P+RF +     G+  + +PFG GRR CPG  LA ++V L +GSLIQCFEW+R
Sbjct: 404 WDDPTSFKPERFEKE----GEAKKLMPFGLGRRACPGSGLAQRLVTLSLGSLIQCFEWER 463

Query: 121 IGEEMVDMAEGTGLTMSKAHPLQAKFR 148
           IGEE VDM EG GLTM KA PL+A  R
Sbjct: 464 IGEEEVDMTEGPGLTMPKARPLEAMCR 486

[Close]

BLAST of MS008260 vs. TAIR 10
Match: AT1G66540.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 189.5 bits (480), Expect = 2.0e-48
Identity = 88/147 (59.86%), Postives = 108/147 (73.47%), Query Frame = 0


Query: 1   DLERLPYLQCVIKETLRMYPVGPLLMPHESSEDCTVGGYHIPRGTMLLVNAWAIHNDGGN 60
           D+  LPYLQ ++ ETLR+YP GPLL+PH SSEDC VGGY +P GTMLLVN WAIH D   
Sbjct: 231 DIPNLPYLQNIVSETLRLYPAGPLLVPHISSEDCKVGGYDMPCGTMLLVNVWAIHRDPRL 290

Query: 61  WEEPAAFRPDRFLEAAAGGGDGLRYVPFGAGRRGCPGEALAVKVVGLVVGSLIQCFEWDR 120
           W++PA+F+P+RF +     G+  + + FG GRR CPG  LA ++V L +GSLIQCFEW+R
Sbjct: 291 WDDPASFKPERFEKE----GETHKLLTFGLGRRACPGSGLARRLVSLSLGSLIQCFEWER 350

Query: 121 IGEEMVDMAEGTGLTMSKAHPLQAKFR 148
           IGEE VDM EG GLTM +A PL A  R
Sbjct: 351 IGEEEVDMTEGGGLTMPRAIPLVAMCR 373

[Close]

BLAST of MS008260 vs. TAIR 10
Match: AT1G66540.2 (Cytochrome P450 superfamily protein )

HSP 1 Score: 189.5 bits (480), Expect = 2.0e-48
Identity = 88/147 (59.86%), Postives = 108/147 (73.47%), Query Frame = 0


Query: 1   DLERLPYLQCVIKETLRMYPVGPLLMPHESSEDCTVGGYHIPRGTMLLVNAWAIHNDGGN 60
           D+  LPYLQ ++ ETLR+YP GPLL+PH SSEDC VGGY +P GTMLLVN WAIH D   
Sbjct: 172 DIPNLPYLQNIVSETLRLYPAGPLLVPHISSEDCKVGGYDMPCGTMLLVNVWAIHRDPRL 231

Query: 61  WEEPAAFRPDRFLEAAAGGGDGLRYVPFGAGRRGCPGEALAVKVVGLVVGSLIQCFEWDR 120
           W++PA+F+P+RF +     G+  + + FG GRR CPG  LA ++V L +GSLIQCFEW+R
Sbjct: 232 WDDPASFKPERFEKE----GETHKLLTFGLGRRACPGSGLARRLVSLSLGSLIQCFEWER 291

Query: 121 IGEEMVDMAEGTGLTMSKAHPLQAKFR 148
           IGEE VDM EG GLTM +A PL A  R
Sbjct: 292 IGEEEVDMTEGGGLTMPRAIPLVAMCR 314

[Close]

BLAST of MS008260 vs. TAIR 10
Match: AT4G37340.1 (cytochrome P450, family 81, subfamily D, polypeptide 3 )

HSP 1 Score: 187.6 bits (475), Expect = 7.6e-48
Identity = 85/153 (55.56%), Postives = 109/153 (71.24%), Query Frame = 0


Query: 1   DLERLPYLQCVIKETLRMYPVGPLLMPHESSEDCTVGGYHIPRGTMLLVNAWAIHNDGGN 60
           DL  LPYLQ ++ E+LR+YP  PLL+PH +SEDC VGGYH+PRGTMLL NAWAIH D   
Sbjct: 344 DLSHLPYLQNIVSESLRLYPASPLLVPHVASEDCKVGGYHMPRGTMLLTNAWAIHRDPKI 403

Query: 61  WEEPAAFRPDRFLEAAAGGGDGLRYVPFGAGRRGCPGEALAVKVVGLVVGSLIQCFEWDR 120
           W++P +F+P+RF +     G+  + + FG GRR CPG  LA ++  L +GSLIQCFEW+R
Sbjct: 404 WDDPTSFKPERFEKE----GEAQKLLGFGLGRRACPGSGLAQRLASLTIGSLIQCFEWER 463

Query: 121 IGEEMVDMAE-GTGLTMSKAHPLQAKFRPSPIL 153
           IGEE VDM E G G+ M KA PL A  +  P++
Sbjct: 464 IGEEEVDMTEGGGGVIMPKAIPLVAMCKARPVV 492

[Close]

BLAST of MS008260 vs. TAIR 10
Match: AT4G37370.1 (cytochrome P450, family 81, subfamily D, polypeptide 8 )

HSP 1 Score: 184.1 bits (466), Expect = 8.4e-47
Identity = 81/150 (54.00%), Postives = 108/150 (72.00%), Query Frame = 0


Query: 1   DLERLPYLQCVIKETLRMYPVGPLLMPHESSEDCTVGGYHIPRGTMLLVNAWAIHNDGGN 60
           D+  LPYLQ ++ ETLR+YP  P+L+PH +SEDC V GY +PRGT+LL N WAIH D   
Sbjct: 343 DISNLPYLQNIVSETLRLYPAAPMLLPHVASEDCKVAGYDMPRGTILLTNVWAIHRDPQL 402

Query: 61  WEEPAAFRPDRFLEAAAGGGDGLRYVPFGAGRRGCPGEALAVKVVGLVVGSLIQCFEWDR 120
           W++P +F+P+RF +     G+  + +PFG GRR CPG  LA +++ L +GSLIQC EW++
Sbjct: 403 WDDPMSFKPERFEKE----GEAQKLMPFGLGRRACPGSGLAHRLINLTLGSLIQCLEWEK 462

Query: 121 IGEEMVDMAEGTGLTMSKAHPLQAKFRPSP 151
           IGEE VDM+EG G+TM KA PL+A  R  P
Sbjct: 463 IGEE-VDMSEGKGVTMPKAKPLEAMCRARP 487

The following BLAST results are available for this feature:

BLAST of MS008260 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022140041.1	1.3e-78	91.56	cytochrome P450 81D1-like [Momordica charantia]	[more]
XP_022142908.1	5.6e-77	90.91	cytochrome P450 81D11-like [Momordica charantia]	[more]
XP_008444122.1	9.0e-67	79.87	PREDICTED: cytochrome P450 81D1-like [Cucumis melo] >TYK19213.1 cytochrome P450 ...	[more]
KAA0052386.1	9.0e-67	79.87	cytochrome P450 81D1-like [Cucumis melo var. makuwa]	[more]
XP_004142688.1	1.5e-66	80.39	cytochrome P450 81Q32 [Cucumis sativus] >KGN54600.1 hypothetical protein Csa_012...	[more]

BLAST of MS008260 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
W8JMU7	3.1e-46	56.05	Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1	[more]
Q9LHA1	1.0e-44	52.90	Cytochrome P450 81D11 OS=Arabidopsis thaliana OX=3702 GN=CYP81D11 PE=2 SV=1	[more]
Q9FG65	1.7e-44	51.95	Cytochrome P450 81D1 OS=Arabidopsis thaliana OX=3702 GN=CYP81D1 PE=2 SV=1	[more]
Q0WTF4	1.4e-41	49.68	Cytochrome P450 81F3 OS=Arabidopsis thaliana OX=3702 GN=CYP81F3 PE=2 SV=1	[more]
O65790	2.3e-41	48.70	Cytochrome P450 81F1 OS=Arabidopsis thaliana OX=3702 GN=CYP81F1 PE=2 SV=2	[more]

BLAST of MS008260 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CEJ7	6.5e-79	91.56	cytochrome P450 81D1-like OS=Momordica charantia OX=3673 GN=LOC111010788 PE=3 SV...	[more]
A0A6J1CPF5	2.7e-77	90.91	cytochrome P450 81D11-like OS=Momordica charantia OX=3673 GN=LOC111012906 PE=3 S...	[more]
A0A5A7UDX9	4.3e-67	79.87	Cytochrome P450 81D1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffo...	[more]
A0A5D3D6T6	4.3e-67	79.87	Cytochrome P450 81D1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo...	[more]
A0A1S3B958	4.3e-67	79.87	cytochrome P450 81D1-like OS=Cucumis melo OX=3656 GN=LOC103487563 PE=3 SV=1	[more]

BLAST of MS008260 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G37360.1	1.4e-49	58.50	cytochrome P450, family 81, subfamily D, polypeptide 2	[more]
AT1G66540.1	2.0e-48	59.86	Cytochrome P450 superfamily protein	[more]
AT1G66540.2	2.0e-48	59.86	Cytochrome P450 superfamily protein	[more]
AT4G37340.1	7.6e-48	55.56	cytochrome P450, family 81, subfamily D, polypeptide 3	[more]
AT4G37370.1	8.4e-47	54.00	cytochrome P450, family 81, subfamily D, polypeptide 8	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001128	Cytochrome P450	PRINTS	PR00385	P450	coord: 10..21 score: 46.12 coord: 95..106 score: 36.17 coord: 86..95 score: 58.17
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 1..142 e-value: 2.3E-43 score: 148.6
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 9..27 score: 36.08 coord: 85..95 score: 55.0 coord: 50..74 score: 37.55 coord: 95..118 score: 36.62
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 1..149 e-value: 1.9E-50 score: 173.8
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 1..148
None	No IPR available	PANTHER	PTHR47947:SF17	CYTOCHROME P450, FAMILY 81, SUBFAMILY D, POLYPEPTIDE 5	coord: 1..151
None	No IPR available	PANTHER	PTHR47947	CYTOCHROME P450 82C3-RELATED	coord: 1..151
IPR017972	Cytochrome P450, conserved site	PROSITE	PS00086	CYTOCHROME_P450	coord: 88..97

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS008260.1	MS008260.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen