MS008229 (gene) Bitter gourd (TR) v1

Overview
NameMS008229
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionAlkaline/neutral invertase
Locationscaffold45: 454754 .. 457387 (+)
RNA-Seq ExpressionMS008229
SyntenyMS008229
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTTGGAGCTTCGCCGCCCAGGCCGAAGTATAGGAGAATTGATAAAATTTCGAAGTTACGGCCGAATTGTTCTAGAATTATAGGGTCTGTACAGGTTAATGATAATTCGAGTAGGAGAGGTTTTGGTTTTTCCAATGGTTATCGGTTGAGTAGAGGTAGTGGTGGTAAAAATAGGCATTTGATTGCAAATGTGGCGTCGGATTTTAGAAATCAGTCGACGTCGGCTGAAGCTCATGTCAATCAGAAGAGCTTTGAGACAATATATATTCAAGGAGGGTTTACAGTGAAGCCACTGGTGATTGAGAGTATTGAGACGAGTCGTGGTTTAGAGAAAGAAGAAAAATCGGCATCGGAGGCTGAGGGGTTGGGTAGTTTAAAGGATTTTAATTATGCTAGGGTTGAGAGAGAGGCGTCTGAGATTGAAAAGGAGGCATGGAGCTTGCTTCGAGACTCTGTTGTGAACTATTGTGGACATCCTGTTGGAACTGTTGCTGCTAATGATCCTGCTGATACTCAGCCACTGAATTACGACCAGGTCTTTGTTCGTGATTTTGTCCCGTCGGCCTTGGCCTTCTTGTTGAATGGAGAAGACGAAATTGTCAAGAATTTTCTCCTGCACACCTTGCAATTACAGGTATATGCCTAAACATTAAGCTTTCCGTTCATCTAAAAGAGGGGACATCTTTTATATGTGTATCTGATCTATTACAAAATTTGATGTAAGATATTAGTGAATTTAGCTGATTGTAGAATGATAGGATCATTACCCACTGTATCTTGACGTATCAAATTTAGAAATACCATTTTTAAGGTCATAATGAAGTCATTCTCTTTATATTCATGAATACTACAGCTCACTTGTCCTTTGTACTGAAATCAACCGTCTTTGATGTTGGTTAGAGTTGGGAGAAGACTGTTGACTGTTACAGCCCCGGGCAAGGGCTGATGCCAGCAAGTTTCAAAGTTAGAAGCCAACCTCTTGATGGAAGTGATGGAGCTTTCGAGGAAGTTCTTGATCCCGATTTTGGTGAATCTGCCATTGGTCGTGTTGCACCGGTTGATTCTGGTAAAGAAACTTCTGTTTTTTCAGTTCTGAAATGGATTCACTTCAATTTTTTCTTCCACATAGAACATGAAATCTTAACCTGTTACTGTGTGTTTTTACTCACACAGGGCTGTGGTGGATTATTTTGTTAAGAGCTTATGGAAAGATCACAGGAGACTACACATTGCAGGAACGGGTCGATGTACAGACAGGCATAAGACTGATCCTTAATCTGTGCTTGACGAATGGGTTTGACATGTTTCCTACTCTGTTAGTTAGTGACGGTTCCTGCATGATCGATAGACGAATGGGCATTCATGGACACCCACTGGAAATTCAAGTAAGAATAAGTAGACCAATAATCAATTATCATTGTGGTTTTTTCAAATATTGAGCAGCAATTCTTAATAGCTATAGTAAACTGTTATCCTCTTAGTTTAGTTCGAGTTCATGTACGGGCAATCGCTTGACGTGGTTGACTTCGTTTTGCAGGCATTATTCTACTCAGCCCTACGCTGCTCCAGAGAGATGTTGATTGTCAACGACGCAACCAAGAATTTGGTGGCTGCCCTGAACAGTAGACTAAGTTCACTCTCCTTCCATATCCGGGAATACTACTGGATCGATATGAACAAAATCAACGAAATTTATCGTTACAAAACGGAGGAGTATTCTTCTGAAGCTGTCAATAAGTTCAATATATATCCTGAACAAATTCCTAGTTGGCTGGTGGATTGGATTCCTGAGGAAGGTGGCTACCTTATTGGCAATCTACAGCCTGCCCACATGGACTTCAGGTTTTTCACGCTCGGAAATCTTTGGTCCATCGTGTCATCTCTCGGGACCCCGAAACAGAACAAGGGCATTCTGAACTTGATTGAAGCCAAATGGGATGACCTTGTGGCTAACATGCCTCTTAAGATATGCTTCCCGGCTATGGAGTACGAGGAATGGCGCATCGTAACCGGCAGTGACCCTAAGAACACGTGAGGCTCTTTCGAGTTTTGAGGCAGTGAAAATTCGTTTGATTTGAAGCAAGTATATAGGAAATTTTTGTAACCAAACGCTGATCTTTTCCTTCGTTTTTTCCCTTTTGGCAGTCCTTGGTCATATCATAATGGGGGATCTTGGCCAACACTGTTATGGCAGGTGAGGCACTTATTATTCAAACTTCATTTTTACCCCTCACCTCAAAACAGTTGATTTTCAACCATTTATTAATCTCACTGTAAATGTTTGTGGGCAATTTTACAGTTCACACTGGCCTGTATGAAGATGGGGAGGCCAGAGCTAGCAAGGAATGCCATTGCAGTGGCTGAGAAGAAGCTTTCGGCCGATCGTTGGCCCGAGTACTATGATATGCGCAGTGCGAGATTAATAGGGAAGCAATCACGGCTCTTCCAAACCTGGACGATCGCTGGTTTCTTGACATCAAAGTTGCTTCTCGAGAACCCAGAAAAGGCGTCTCTGTTGTATTGGGAGGAGGATTACGAGCTCCTTCAGAGCTGCGTCTGTGCGCTCGGCAAAGCCAACGGGAACAGGTGCTCTCGCCAGCGCCACCCTGCAAGACCAGACGATCACGACCAT

mRNA sequence

ATGTTTGGAGCTTCGCCGCCCAGGCCGAAGTATAGGAGAATTGATAAAATTTCGAAGTTACGGCCGAATTGTTCTAGAATTATAGGGTCTGTACAGGTTAATGATAATTCGAGTAGGAGAGGTTTTGGTTTTTCCAATGGTTATCGGTTGAGTAGAGGTAGTGGTGGTAAAAATAGGCATTTGATTGCAAATGTGGCGTCGGATTTTAGAAATCAGTCGACGTCGGCTGAAGCTCATGTCAATCAGAAGAGCTTTGAGACAATATATATTCAAGGAGGGTTTACAGTGAAGCCACTGGTGATTGAGAGTATTGAGACGAGTCGTGGTTTAGAGAAAGAAGAAAAATCGGCATCGGAGGCTGAGGGGTTGGGTAGTTTAAAGGATTTTAATTATGCTAGGGTTGAGAGAGAGGCGTCTGAGATTGAAAAGGAGGCATGGAGCTTGCTTCGAGACTCTGTTGTGAACTATTGTGGACATCCTGTTGGAACTGTTGCTGCTAATGATCCTGCTGATACTCAGCCACTGAATTACGACCAGGTCTTTGTTCGTGATTTTGTCCCGTCGGCCTTGGCCTTCTTGTTGAATGGAGAAGACGAAATTGTCAAGAATTTTCTCCTGCACACCTTGCAATTACAGAGTTGGGAGAAGACTGTTGACTGTTACAGCCCCGGGCAAGGGCTGATGCCAGCAAGTTTCAAAGTTAGAAGCCAACCTCTTGATGGAAGTGATGGAGCTTTCGAGGAAGTTCTTGATCCCGATTTTGGTGAATCTGCCATTGGTCGTGTTGCACCGGTTGATTCTGGGCTGTGGTGGATTATTTTGTTAAGAGCTTATGGAAAGATCACAGGAGACTACACATTGCAGGAACGGGTCGATGTACAGACAGGCATAAGACTGATCCTTAATCTGTGCTTGACGAATGGGTTTGACATGTTTCCTACTCTGTTAGTTAGTGACGGTTCCTGCATGATCGATAGACGAATGGGCATTCATGGACACCCACTGGAAATTCAAGCATTATTCTACTCAGCCCTACGCTGCTCCAGAGAGATGTTGATTGTCAACGACGCAACCAAGAATTTGGTGGCTGCCCTGAACAGTAGACTAAGTTCACTCTCCTTCCATATCCGGGAATACTACTGGATCGATATGAACAAAATCAACGAAATTTATCGTTACAAAACGGAGGAGTATTCTTCTGAAGCTGTCAATAAGTTCAATATATATCCTGAACAAATTCCTAGTTGGCTGGTGGATTGGATTCCTGAGGAAGGTGGCTACCTTATTGGCAATCTACAGCCTGCCCACATGGACTTCAGGTTTTTCACGCTCGGAAATCTTTGGTCCATCGTGTCATCTCTCGGGACCCCGAAACAGAACAAGGGCATTCTGAACTTGATTGAAGCCAAATGGGATGACCTTGTGGCTAACATGCCTCTTAAGATATGCTTCCCGGCTATGGAGTACGAGGAATGGCGCATCGTAACCGGCAGTGACCCTAAGAACACTCCTTGGTCATATCATAATGGGGGATCTTGGCCAACACTGTTATGGCAGTTCACACTGGCCTGTATGAAGATGGGGAGGCCAGAGCTAGCAAGGAATGCCATTGCAGTGGCTGAGAAGAAGCTTTCGGCCGATCGTTGGCCCGAGTACTATGATATGCGCAGTGCGAGATTAATAGGGAAGCAATCACGGCTCTTCCAAACCTGGACGATCGCTGGTTTCTTGACATCAAAGTTGCTTCTCGAGAACCCAGAAAAGGCGTCTCTGTTGTATTGGGAGGAGGATTACGAGCTCCTTCAGAGCTGCGTCTGTGCGCTCGGCAAAGCCAACGGGAACAGGTGCTCTCGCCAGCGCCACCCTGCAAGACCAGACGATCACGACCAT

Coding sequence (CDS)

ATGTTTGGAGCTTCGCCGCCCAGGCCGAAGTATAGGAGAATTGATAAAATTTCGAAGTTACGGCCGAATTGTTCTAGAATTATAGGGTCTGTACAGGTTAATGATAATTCGAGTAGGAGAGGTTTTGGTTTTTCCAATGGTTATCGGTTGAGTAGAGGTAGTGGTGGTAAAAATAGGCATTTGATTGCAAATGTGGCGTCGGATTTTAGAAATCAGTCGACGTCGGCTGAAGCTCATGTCAATCAGAAGAGCTTTGAGACAATATATATTCAAGGAGGGTTTACAGTGAAGCCACTGGTGATTGAGAGTATTGAGACGAGTCGTGGTTTAGAGAAAGAAGAAAAATCGGCATCGGAGGCTGAGGGGTTGGGTAGTTTAAAGGATTTTAATTATGCTAGGGTTGAGAGAGAGGCGTCTGAGATTGAAAAGGAGGCATGGAGCTTGCTTCGAGACTCTGTTGTGAACTATTGTGGACATCCTGTTGGAACTGTTGCTGCTAATGATCCTGCTGATACTCAGCCACTGAATTACGACCAGGTCTTTGTTCGTGATTTTGTCCCGTCGGCCTTGGCCTTCTTGTTGAATGGAGAAGACGAAATTGTCAAGAATTTTCTCCTGCACACCTTGCAATTACAGAGTTGGGAGAAGACTGTTGACTGTTACAGCCCCGGGCAAGGGCTGATGCCAGCAAGTTTCAAAGTTAGAAGCCAACCTCTTGATGGAAGTGATGGAGCTTTCGAGGAAGTTCTTGATCCCGATTTTGGTGAATCTGCCATTGGTCGTGTTGCACCGGTTGATTCTGGGCTGTGGTGGATTATTTTGTTAAGAGCTTATGGAAAGATCACAGGAGACTACACATTGCAGGAACGGGTCGATGTACAGACAGGCATAAGACTGATCCTTAATCTGTGCTTGACGAATGGGTTTGACATGTTTCCTACTCTGTTAGTTAGTGACGGTTCCTGCATGATCGATAGACGAATGGGCATTCATGGACACCCACTGGAAATTCAAGCATTATTCTACTCAGCCCTACGCTGCTCCAGAGAGATGTTGATTGTCAACGACGCAACCAAGAATTTGGTGGCTGCCCTGAACAGTAGACTAAGTTCACTCTCCTTCCATATCCGGGAATACTACTGGATCGATATGAACAAAATCAACGAAATTTATCGTTACAAAACGGAGGAGTATTCTTCTGAAGCTGTCAATAAGTTCAATATATATCCTGAACAAATTCCTAGTTGGCTGGTGGATTGGATTCCTGAGGAAGGTGGCTACCTTATTGGCAATCTACAGCCTGCCCACATGGACTTCAGGTTTTTCACGCTCGGAAATCTTTGGTCCATCGTGTCATCTCTCGGGACCCCGAAACAGAACAAGGGCATTCTGAACTTGATTGAAGCCAAATGGGATGACCTTGTGGCTAACATGCCTCTTAAGATATGCTTCCCGGCTATGGAGTACGAGGAATGGCGCATCGTAACCGGCAGTGACCCTAAGAACACTCCTTGGTCATATCATAATGGGGGATCTTGGCCAACACTGTTATGGCAGTTCACACTGGCCTGTATGAAGATGGGGAGGCCAGAGCTAGCAAGGAATGCCATTGCAGTGGCTGAGAAGAAGCTTTCGGCCGATCGTTGGCCCGAGTACTATGATATGCGCAGTGCGAGATTAATAGGGAAGCAATCACGGCTCTTCCAAACCTGGACGATCGCTGGTTTCTTGACATCAAAGTTGCTTCTCGAGAACCCAGAAAAGGCGTCTCTGTTGTATTGGGAGGAGGATTACGAGCTCCTTCAGAGCTGCGTCTGTGCGCTCGGCAAAGCCAACGGGAACAGGTGCTCTCGCCAGCGCCACCCTGCAAGACCAGACGATCACGACCAT

Protein sequence

MFGASPPRPKYRRIDKISKLRPNCSRIIGSVQVNDNSSRRGFGFSNGYRLSRGSGGKNRHLIANVASDFRNQSTSAEAHVNQKSFETIYIQGGFTVKPLVIESIETSRGLEKEEKSASEAEGLGSLKDFNYARVEREASEIEKEAWSLLRDSVVNYCGHPVGTVAANDPADTQPLNYDQVFVRDFVPSALAFLLNGEDEIVKNFLLHTLQLQSWEKTVDCYSPGQGLMPASFKVRSQPLDGSDGAFEEVLDPDFGESAIGRVAPVDSGLWWIILLRAYGKITGDYTLQERVDVQTGIRLILNLCLTNGFDMFPTLLVSDGSCMIDRRMGIHGHPLEIQALFYSALRCSREMLIVNDATKNLVAALNSRLSSLSFHIREYYWIDMNKINEIYRYKTEEYSSEAVNKFNIYPEQIPSWLVDWIPEEGGYLIGNLQPAHMDFRFFTLGNLWSIVSSLGTPKQNKGILNLIEAKWDDLVANMPLKICFPAMEYEEWRIVTGSDPKNTPWSYHNGGSWPTLLWQFTLACMKMGRPELARNAIAVAEKKLSADRWPEYYDMRSARLIGKQSRLFQTWTIAGFLTSKLLLENPEKASLLYWEEDYELLQSCVCALGKANGNRCSRQRHPARPDDHDH
Homology
BLAST of MS008229 vs. NCBI nr
Match: XP_022138141.1 (alkaline/neutral invertase A, mitochondrial [Momordica charantia])

HSP 1 Score: 1297.0 bits (3355), Expect = 0.0e+00
Identity = 629/630 (99.84%), Postives = 630/630 (100.00%), Query Frame = 0

Query: 1   MFGASPPRPKYRRIDKISKLRPNCSRIIGSVQVNDNSSRRGFGFSNGYRLSRGSGGKNRH 60
           MFGASPPRPKYRRIDKISKLRPNCSRIIGSVQVNDNSSRRGFGFSNGYRLSRGSGGKNRH
Sbjct: 26  MFGASPPRPKYRRIDKISKLRPNCSRIIGSVQVNDNSSRRGFGFSNGYRLSRGSGGKNRH 85

Query: 61  LIANVASDFRNQSTSAEAHVNQKSFETIYIQGGFTVKPLVIESIETSRGLEKEEKSASEA 120
           LIANVASDFRNQSTSAEAHVNQKSFETIYIQGGFTVKPLVIESIET+RGLEKEEKSASEA
Sbjct: 86  LIANVASDFRNQSTSAEAHVNQKSFETIYIQGGFTVKPLVIESIETNRGLEKEEKSASEA 145

Query: 121 EGLGSLKDFNYARVEREASEIEKEAWSLLRDSVVNYCGHPVGTVAANDPADTQPLNYDQV 180
           EGLGSLKDFNYARVEREASEIEKEAWSLLRDSVVNYCGHPVGTVAANDPADTQPLNYDQV
Sbjct: 146 EGLGSLKDFNYARVEREASEIEKEAWSLLRDSVVNYCGHPVGTVAANDPADTQPLNYDQV 205

Query: 181 FVRDFVPSALAFLLNGEDEIVKNFLLHTLQLQSWEKTVDCYSPGQGLMPASFKVRSQPLD 240
           FVRDFVPSALAFLLNGEDEIVKNFLLHTLQLQSWEKTVDCYSPGQGLMPASFKVRSQPLD
Sbjct: 206 FVRDFVPSALAFLLNGEDEIVKNFLLHTLQLQSWEKTVDCYSPGQGLMPASFKVRSQPLD 265

Query: 241 GSDGAFEEVLDPDFGESAIGRVAPVDSGLWWIILLRAYGKITGDYTLQERVDVQTGIRLI 300
           GSDGAFEEVLDPDFGESAIGRVAPVDSGLWWIILLRAYGKITGDYTLQERVDVQTGIRLI
Sbjct: 266 GSDGAFEEVLDPDFGESAIGRVAPVDSGLWWIILLRAYGKITGDYTLQERVDVQTGIRLI 325

Query: 301 LNLCLTNGFDMFPTLLVSDGSCMIDRRMGIHGHPLEIQALFYSALRCSREMLIVNDATKN 360
           LNLCLTNGFDMFPTLLVSDGSCMIDRRMGIHGHPLEIQALFYSALRCSREMLIVNDATKN
Sbjct: 326 LNLCLTNGFDMFPTLLVSDGSCMIDRRMGIHGHPLEIQALFYSALRCSREMLIVNDATKN 385

Query: 361 LVAALNSRLSSLSFHIREYYWIDMNKINEIYRYKTEEYSSEAVNKFNIYPEQIPSWLVDW 420
           LVAALNSRLSSLSFHIREYYWIDMNKINEIYRYKTEEYSSEAVNKFNIYPEQIPSWLVDW
Sbjct: 386 LVAALNSRLSSLSFHIREYYWIDMNKINEIYRYKTEEYSSEAVNKFNIYPEQIPSWLVDW 445

Query: 421 IPEEGGYLIGNLQPAHMDFRFFTLGNLWSIVSSLGTPKQNKGILNLIEAKWDDLVANMPL 480
           IPEEGGYLIGNLQPAHMDFRFFTLGNLWSIVSSLGTPKQNKGILNLIEAKWDDLVANMPL
Sbjct: 446 IPEEGGYLIGNLQPAHMDFRFFTLGNLWSIVSSLGTPKQNKGILNLIEAKWDDLVANMPL 505

Query: 481 KICFPAMEYEEWRIVTGSDPKNTPWSYHNGGSWPTLLWQFTLACMKMGRPELARNAIAVA 540
           KICFPAMEYEEWRIVTGSDPKNTPWSYHNGGSWPTLLWQFTLACMKMGRPELARNAIAVA
Sbjct: 506 KICFPAMEYEEWRIVTGSDPKNTPWSYHNGGSWPTLLWQFTLACMKMGRPELARNAIAVA 565

Query: 541 EKKLSADRWPEYYDMRSARLIGKQSRLFQTWTIAGFLTSKLLLENPEKASLLYWEEDYEL 600
           EKKLSADRWPEYYDMRSARLIGKQSRLFQTWTIAGFLTSKLLLENPEKASLLYWEEDYEL
Sbjct: 566 EKKLSADRWPEYYDMRSARLIGKQSRLFQTWTIAGFLTSKLLLENPEKASLLYWEEDYEL 625

Query: 601 LQSCVCALGKANGNRCSRQRHPARPDDHDH 631
           LQSCVCALGKANGNRCSRQRHPARPDDHDH
Sbjct: 626 LQSCVCALGKANGNRCSRQRHPARPDDHDH 655

BLAST of MS008229 vs. NCBI nr
Match: XP_038875028.1 (alkaline/neutral invertase A, mitochondrial [Benincasa hispida])

HSP 1 Score: 1147.5 bits (2967), Expect = 0.0e+00
Identity = 554/626 (88.50%), Postives = 585/626 (93.45%), Query Frame = 0

Query: 1   MFGA-SPPRPKYRRIDKISKLRPNCSRIIGSVQVNDNSSRRGFGFSNGYRLSRGSGGKNR 60
           MFG  + P+PKY+RI ++SKL PN  +IIGSVQV DN +RR F FSNGYRL +GS G+NR
Sbjct: 26  MFGTIASPKPKYKRISRLSKLEPNGCKIIGSVQVLDNLNRRCFSFSNGYRLYKGSSGRNR 85

Query: 61  HLIANVASDFRNQSTSAEAHVNQKSFETIYIQGGFTVKPLVIESIETSRGLEKEEKSASE 120
            LIANVASDFRNQSTSAE HV QK+F+TIYIQGGF VKPL+I SIET   + KE+     
Sbjct: 86  CLIANVASDFRNQSTSAEPHVKQKNFDTIYIQGGFKVKPLLIGSIETGHDIVKED----- 145

Query: 121 AEGLGSLKDFNYARVEREASEIEKEAWSLLRDSVVNYCGHPVGTVAANDPADTQPLNYDQ 180
            EGLGSLK  NY+RVERE S IEKEAWSLLRDSVV YCGHPVGTVAANDPADTQPLNYDQ
Sbjct: 146 -EGLGSLKGSNYSRVEREVSMIEKEAWSLLRDSVVYYCGHPVGTVAANDPADTQPLNYDQ 205

Query: 181 VFVRDFVPSALAFLLNGEDEIVKNFLLHTLQLQSWEKTVDCYSPGQGLMPASFKVRSQPL 240
           VFVRDFVPSALAFLLNGE+EIVKNFLLHTLQLQSWEKTVDCYSPGQGLMPASFKVRSQPL
Sbjct: 206 VFVRDFVPSALAFLLNGEEEIVKNFLLHTLQLQSWEKTVDCYSPGQGLMPASFKVRSQPL 265

Query: 241 DGSDGAFEEVLDPDFGESAIGRVAPVDSGLWWIILLRAYGKITGDYTLQERVDVQTGIRL 300
           DGSDGAFEEVLDPDFGESAIGRVAPVDSGLWWIILLRAYGKITGDYTLQERVDVQTGIRL
Sbjct: 266 DGSDGAFEEVLDPDFGESAIGRVAPVDSGLWWIILLRAYGKITGDYTLQERVDVQTGIRL 325

Query: 301 ILNLCLTNGFDMFPTLLVSDGSCMIDRRMGIHGHPLEIQALFYSALRCSREMLIVNDATK 360
           ILNLCLTNGFDMFPTLLVSDGSCMIDRRMGIHGHPLEIQALFYSALRCSREMLIVN++TK
Sbjct: 326 ILNLCLTNGFDMFPTLLVSDGSCMIDRRMGIHGHPLEIQALFYSALRCSREMLIVNESTK 385

Query: 361 NLVAALNSRLSSLSFHIREYYWIDMNKINEIYRYKTEEYSSEAVNKFNIYPEQIPSWLVD 420
           NLV ALN+RLS+LSFHIREYYW+D NKINEIYRYKTEEYS++AVNKFNIYPEQIPSWLVD
Sbjct: 386 NLVVALNNRLSALSFHIREYYWVDKNKINEIYRYKTEEYSTDAVNKFNIYPEQIPSWLVD 445

Query: 421 WIPEEGGYLIGNLQPAHMDFRFFTLGNLWSIVSSLGTPKQNKGILNLIEAKWDDLVANMP 480
           WIPEEGGYLIGNLQPAHMDFRFFTLGNLWS+VSSLGTPKQN+GILNLIEAKWDDLVANMP
Sbjct: 446 WIPEEGGYLIGNLQPAHMDFRFFTLGNLWSVVSSLGTPKQNEGILNLIEAKWDDLVANMP 505

Query: 481 LKICFPAMEYEEWRIVTGSDPKNTPWSYHNGGSWPTLLWQFTLACMKMGRPELARNAIAV 540
           LKICFPAMEYEEWRIVTGSDPKNTPWSYHNGGSWPTLLWQFTLACMKMGRPELARNAIAV
Sbjct: 506 LKICFPAMEYEEWRIVTGSDPKNTPWSYHNGGSWPTLLWQFTLACMKMGRPELARNAIAV 565

Query: 541 AEKKLSADRWPEYYDMRSARLIGKQSRLFQTWTIAGFLTSKLLLENPEKASLLYWEEDYE 600
           AEKKL+ DRWPEYYDMRSARL+GKQSRLFQTWTIAGFLTSKLLLENPEKASLL+WEEDYE
Sbjct: 566 AEKKLAVDRWPEYYDMRSARLVGKQSRLFQTWTIAGFLTSKLLLENPEKASLLFWEEDYE 625

Query: 601 LLQSCVCALGKANGNRCSRQRHPARP 626
           +LQ+CVCALGKAN N+CSR RH   P
Sbjct: 626 ILQNCVCALGKANRNKCSRHRHRQHP 645

BLAST of MS008229 vs. NCBI nr
Match: XP_008438972.1 (PREDICTED: alkaline/neutral invertase A, mitochondrial [Cucumis melo])

HSP 1 Score: 1143.3 bits (2956), Expect = 0.0e+00
Identity = 550/631 (87.16%), Postives = 584/631 (92.55%), Query Frame = 0

Query: 1   MFGA-SPPRPKYRRIDKISKLRPNCSRIIGSVQVNDNSSRRGFGFSNGYRLSRGSGGKNR 60
           MFG  + P+ KY+RI + SKL PN  ++IGSVQV D  SRR F FSNGYRL +GS  +NR
Sbjct: 26  MFGTIASPKLKYKRIGRFSKLEPNGCKVIGSVQVVDKLSRRCFCFSNGYRLYKGSSDRNR 85

Query: 61  HLIANVASDFRNQSTSAEAHVNQKSFETIYIQGGFTVKPLVIESIETSRGLEKEEKSASE 120
           HLIANVASDFRNQSTSAE++V QKSF+ IYI GGF VKPL IESIET   + KE+K  SE
Sbjct: 86  HLIANVASDFRNQSTSAESYVKQKSFDAIYINGGFKVKPLEIESIETGHDIVKEDKKVSE 145

Query: 121 AEGLGSLKDFNYARVEREASEIEKEAWSLLRDSVVNYCGHPVGTVAANDPADTQPLNYDQ 180
            EGLGSLK  NY+RVERE S+IEKEAW LLR+SVV YCGHPVGTVAANDPAD QPLNYDQ
Sbjct: 146 VEGLGSLKGSNYSRVERELSKIEKEAWDLLRNSVVFYCGHPVGTVAANDPADAQPLNYDQ 205

Query: 181 VFVRDFVPSALAFLLNGEDEIVKNFLLHTLQLQSWEKTVDCYSPGQGLMPASFKVRSQPL 240
           VFVRDFVPSALAFLLNGE+EIVKNFLLHTLQLQSWEKTVDCYSPGQGLMPASFKVRSQPL
Sbjct: 206 VFVRDFVPSALAFLLNGEEEIVKNFLLHTLQLQSWEKTVDCYSPGQGLMPASFKVRSQPL 265

Query: 241 DGSDGAFEEVLDPDFGESAIGRVAPVDSGLWWIILLRAYGKITGDYTLQERVDVQTGIRL 300
           DGSDGAFEEVLDPDFGESAIGRVAPVDSGLWWIILLRAYGKITGDYTLQERVDVQTGIRL
Sbjct: 266 DGSDGAFEEVLDPDFGESAIGRVAPVDSGLWWIILLRAYGKITGDYTLQERVDVQTGIRL 325

Query: 301 ILNLCLTNGFDMFPTLLVSDGSCMIDRRMGIHGHPLEIQALFYSALRCSREMLIVNDATK 360
           ILNLCLTNGFDMFPTLLVSDGSCMIDRRMGIHGHPLEIQALFYSALRCSREMLIVND+TK
Sbjct: 326 ILNLCLTNGFDMFPTLLVSDGSCMIDRRMGIHGHPLEIQALFYSALRCSREMLIVNDSTK 385

Query: 361 NLVAALNSRLSSLSFHIREYYWIDMNKINEIYRYKTEEYSSEAVNKFNIYPEQIPSWLVD 420
           NLV  LN+RLS+LSFHIREYYW+D NKINEIYRYKTEEYS++AVNKFNIYPEQIPSWLVD
Sbjct: 386 NLVVELNNRLSALSFHIREYYWVDKNKINEIYRYKTEEYSTDAVNKFNIYPEQIPSWLVD 445

Query: 421 WIPEEGGYLIGNLQPAHMDFRFFTLGNLWSIVSSLGTPKQNKGILNLIEAKWDDLVANMP 480
           WIPEEGGY IGNLQPAHMDFRFFTLGNLWS+VSSLGTPKQN+GILNLIEAKWDDLVANMP
Sbjct: 446 WIPEEGGYFIGNLQPAHMDFRFFTLGNLWSVVSSLGTPKQNEGILNLIEAKWDDLVANMP 505

Query: 481 LKICFPAMEYEEWRIVTGSDPKNTPWSYHNGGSWPTLLWQFTLACMKMGRPELARNAIAV 540
           LKICFPAMEYEEWRI+TGSDPKNTPWSYHNGGSWPTLLWQFTLAC+KMGRPELARNAIAV
Sbjct: 506 LKICFPAMEYEEWRIITGSDPKNTPWSYHNGGSWPTLLWQFTLACIKMGRPELARNAIAV 565

Query: 541 AEKKLSADRWPEYYDMRSARLIGKQSRLFQTWTIAGFLTSKLLLENPEKASLLYWEEDYE 600
           AEKKLS DRWPEYYDMRSARLIGKQSRLFQTWTIAGFLTSKLLLENPEKASLL+WEEDYE
Sbjct: 566 AEKKLSVDRWPEYYDMRSARLIGKQSRLFQTWTIAGFLTSKLLLENPEKASLLFWEEDYE 625

Query: 601 LLQSCVCALGKANGNRCSRQRHPARPDDHDH 631
           +LQ+CVCALGK NGN+CSR R   +P + +H
Sbjct: 626 ILQNCVCALGKGNGNKCSRHRQHPKPSNPNH 656

BLAST of MS008229 vs. NCBI nr
Match: XP_011651089.1 (alkaline/neutral invertase A, mitochondrial [Cucumis sativus] >KGN57182.1 hypothetical protein Csa_010368 [Cucumis sativus])

HSP 1 Score: 1130.9 bits (2924), Expect = 0.0e+00
Identity = 543/626 (86.74%), Postives = 580/626 (92.65%), Query Frame = 0

Query: 1   MFGA-SPPRPKYRRIDKISKLRPNCSRIIGSVQVNDNSSRRGFGFSNGYRLSRGSGGKNR 60
           MFG  + P+ KY+RI + SKL PN  +I GSVQV DN SRR   FSNGYRL +GS  +NR
Sbjct: 26  MFGTIASPKLKYKRIGRFSKLEPNGCKITGSVQVVDNLSRRCICFSNGYRLYKGSNDRNR 85

Query: 61  HLIANVASDFRNQSTSAEAHVNQKSFETIYIQGGFTVKPLVIESIETSRGLEKEEKSASE 120
            LIANVASDFRNQSTS+E++V QKSF+TIYI GGF VKPL IESIET   + KE+K  SE
Sbjct: 86  CLIANVASDFRNQSTSSESYVKQKSFDTIYINGGFKVKPLEIESIETGHDIVKEDKKVSE 145

Query: 121 AEGLGSLKDFNYARVEREASEIEKEAWSLLRDSVVNYCGHPVGTVAANDPADTQPLNYDQ 180
            EGLGSLK  NY+RVERE S+IEKEAW LLR+SVV YCGHPVGTVAANDPAD+QPLNYDQ
Sbjct: 146 VEGLGSLKGSNYSRVEREVSKIEKEAWDLLRNSVVFYCGHPVGTVAANDPADSQPLNYDQ 205

Query: 181 VFVRDFVPSALAFLLNGEDEIVKNFLLHTLQLQSWEKTVDCYSPGQGLMPASFKVRSQPL 240
           VFVRDF+PSALAFLLNGE+EIVKNFLLHTLQLQSWEKTVDCYSPGQGLMPASFKVRSQPL
Sbjct: 206 VFVRDFIPSALAFLLNGEEEIVKNFLLHTLQLQSWEKTVDCYSPGQGLMPASFKVRSQPL 265

Query: 241 DGSDGAFEEVLDPDFGESAIGRVAPVDSGLWWIILLRAYGKITGDYTLQERVDVQTGIRL 300
           DGSDGAFEEVLDPDFGESAIGRVAPVDSGLWWIIL+RAYGKITGDYTLQERVDVQTGIRL
Sbjct: 266 DGSDGAFEEVLDPDFGESAIGRVAPVDSGLWWIILVRAYGKITGDYTLQERVDVQTGIRL 325

Query: 301 ILNLCLTNGFDMFPTLLVSDGSCMIDRRMGIHGHPLEIQALFYSALRCSREMLIVNDATK 360
           ILNLCLTNGFDMFPTLLVSDGSCMIDRRMGIHGHPLEIQALFYSALRCSREMLIVND+TK
Sbjct: 326 ILNLCLTNGFDMFPTLLVSDGSCMIDRRMGIHGHPLEIQALFYSALRCSREMLIVNDSTK 385

Query: 361 NLVAALNSRLSSLSFHIREYYWIDMNKINEIYRYKTEEYSSEAVNKFNIYPEQIPSWLVD 420
           NLV  LN+RLS+LSFHIREYYW+D NKINEIYRYKTEEYSS+AVNKFNIYPEQIPSWLVD
Sbjct: 386 NLVVELNNRLSALSFHIREYYWVDKNKINEIYRYKTEEYSSDAVNKFNIYPEQIPSWLVD 445

Query: 421 WIPEEGGYLIGNLQPAHMDFRFFTLGNLWSIVSSLGTPKQNKGILNLIEAKWDDLVANMP 480
           WIPEEGGY +GNLQPAHMDFRFFTLGNLWSIVSSLGTPKQN+GILNLIEAKWDDLVANMP
Sbjct: 446 WIPEEGGYFMGNLQPAHMDFRFFTLGNLWSIVSSLGTPKQNEGILNLIEAKWDDLVANMP 505

Query: 481 LKICFPAMEYEEWRIVTGSDPKNTPWSYHNGGSWPTLLWQFTLACMKMGRPELARNAIAV 540
           LKICFPAMEYEEWRI+TGSDPKNTPWSYHNGGSWPTLLWQFTLAC+KMGRPE+ARNAIAV
Sbjct: 506 LKICFPAMEYEEWRIITGSDPKNTPWSYHNGGSWPTLLWQFTLACIKMGRPEVARNAIAV 565

Query: 541 AEKKLSADRWPEYYDMRSARLIGKQSRLFQTWTIAGFLTSKLLLENPEKASLLYWEEDYE 600
           AEKKLS DRWPEYYDMRSARLIGKQSRLFQTWTIAGFLTSKLLLENPEKASLL+WEEDY+
Sbjct: 566 AEKKLSIDRWPEYYDMRSARLIGKQSRLFQTWTIAGFLTSKLLLENPEKASLLFWEEDYD 625

Query: 601 LLQSCVCALGKANGNRCSRQRHPARP 626
           +LQ+C+CAL K NGN+CSR R   +P
Sbjct: 626 ILQNCICALSKGNGNKCSRHRQHPKP 651

BLAST of MS008229 vs. NCBI nr
Match: XP_022956128.1 (alkaline/neutral invertase A, mitochondrial-like [Cucurbita moschata])

HSP 1 Score: 1110.5 bits (2871), Expect = 0.0e+00
Identity = 544/633 (85.94%), Postives = 581/633 (91.79%), Query Frame = 0

Query: 1   MFG-ASPPRPKYRRIDKISKLRPNCSRIIGSVQVNDNSSRRGFGFSNGYRLSRGSGGKNR 60
           MFG  S  + KYRRI + SKL P+  +IIGSVQV  + +RR F  SN +RL +G+ G+NR
Sbjct: 26  MFGTTSLAKAKYRRIGRFSKLEPSGRKIIGSVQVVGDLNRRCFSCSNLHRLYKGNSGRNR 85

Query: 61  HLIANVASDFRNQSTSAEAHVNQKSFETIYIQGGFTVKPLVIESIETSRGLEKEEKSASE 120
            LIANVASDFRNQSTSAE HV QKSFE IYIQGGF VKPLVIESIET   L K+EK  SE
Sbjct: 86  CLIANVASDFRNQSTSAEPHVKQKSFERIYIQGGFKVKPLVIESIETD--LVKDEKKVSE 145

Query: 121 AEGLGSLKDFNYARVEREASEIEKEAWSLLRDSVVNYCGHPVGTVAANDPADTQPLNYDQ 180
            E L  LK    +RVERE S+IEKEAW+LLRDSVVNYCGHPVGTVAANDP+DTQPLNYDQ
Sbjct: 146 VEELSGLKG---SRVEREVSKIEKEAWNLLRDSVVNYCGHPVGTVAANDPSDTQPLNYDQ 205

Query: 181 VFVRDFVPSALAFLLNGEDEIVKNFLLHTLQLQSWEKTVDCYSPGQGLMPASFKVRSQPL 240
           VFVRDFVPSALAFLLNGE+EIVKNFLLHTLQLQSWEKTVDCYSPGQGLMPASFKVRSQPL
Sbjct: 206 VFVRDFVPSALAFLLNGEEEIVKNFLLHTLQLQSWEKTVDCYSPGQGLMPASFKVRSQPL 265

Query: 241 DGSDGAFEEVLDPDFGESAIGRVAPVDSGLWWIILLRAYGKITGDYTLQERVDVQTGIRL 300
           DGSDGAFEEVLDPDFGESAIGRVAPVDSGLWWIILLRAYGKITGDYTLQER+DVQTGIRL
Sbjct: 266 DGSDGAFEEVLDPDFGESAIGRVAPVDSGLWWIILLRAYGKITGDYTLQERIDVQTGIRL 325

Query: 301 ILNLCLTNGFDMFPTLLVSDGSCMIDRRMGIHGHPLEIQALFYSALRCSREMLIVNDATK 360
           ILNLCLTNGFDMFPTLLV DGSCMIDRRMGIHGHPLEIQALFYSALRCSREMLIVND+TK
Sbjct: 326 ILNLCLTNGFDMFPTLLVGDGSCMIDRRMGIHGHPLEIQALFYSALRCSREMLIVNDSTK 385

Query: 361 NLVAALNSRLSSLSFHIREYYWIDMNKINEIYRYKTEEYSSEAVNKFNIYPEQIPSWLVD 420
           NLVAALN+RLS+LSFHIREY+W+D NK+NEIYRY+TEEYS++AVNKFNIYPEQIP WLVD
Sbjct: 386 NLVAALNNRLSALSFHIREYFWVDKNKLNEIYRYRTEEYSTDAVNKFNIYPEQIPGWLVD 445

Query: 421 WIPEEGGYLIGNLQPAHMDFRFFTLGNLWSIVSSLGTPKQNKGILNLIEAKWDDLVANMP 480
           WIPEEGGYLIGNLQPAHMDFRFFTLGNLWSIVSSLGTP+QN+GILNLIEAKWDDLVANMP
Sbjct: 446 WIPEEGGYLIGNLQPAHMDFRFFTLGNLWSIVSSLGTPQQNEGILNLIEAKWDDLVANMP 505

Query: 481 LKICFPAMEYEEWRIVTGSDPKNTPWSYHNGGSWPTLLWQFTLACMKMGRPELARNAIAV 540
           LKICFPAME+EEWRI+TGSDPKNTPWSYHNGGSWPTLLWQFTLACMKMGRPELAR AIAV
Sbjct: 506 LKICFPAMEHEEWRIITGSDPKNTPWSYHNGGSWPTLLWQFTLACMKMGRPELARKAIAV 565

Query: 541 AEKKLSADRWPEYYDMRSARLIGKQSRLFQTWTIAGFLTSKLLLENPEKASLLYWEEDYE 600
           AEKKLSADRWPEYYDMRSA LIGKQSRLFQTWTIAGFLTSKLLL+NPEKASLL+WEEDYE
Sbjct: 566 AEKKLSADRWPEYYDMRSASLIGKQSRLFQTWTIAGFLTSKLLLDNPEKASLLFWEEDYE 625

Query: 601 LLQSCVCALGKANGNRCSRQRH--PARPDDHDH 631
           LLQ CVC LGKANGN+CSR RH    +P++ +H
Sbjct: 626 LLQGCVCVLGKANGNKCSRHRHRQHRKPNNLNH 653

BLAST of MS008229 vs. ExPASy Swiss-Prot
Match: B9DFA8 (Alkaline/neutral invertase C, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=INVC PE=1 SV=1)

HSP 1 Score: 899.0 bits (2322), Expect = 3.0e-260
Identity = 439/647 (67.85%), Postives = 522/647 (80.68%), Query Frame = 0

Query: 1   MFGASPP---------RPKYRRIDKIS-KLRPNCSRII--GSVQVNDNSSRRGFGFSNGY 60
           +FG SPP         +    +ID  S +   +C RI+   +   + +S   G G S   
Sbjct: 27  LFGFSPPNSGKFINSSKLHCTKIDSRSIRSGIHCRRIVLDRNAFCDSDSISWGGGGSRVL 86

Query: 61  RLSRGSGGKNRH--LIANVASDFRNQSTSA-EAHVNQKSFETIYIQGGFTVKPLVIESIE 120
           R    S G+ R   +I +VASDFRN STS+ ++HVN KSFE+++      VKPLV + +E
Sbjct: 87  RARGSSRGRGRGVLVIPHVASDFRNYSTSSLDSHVNDKSFESMF------VKPLVFKEVE 146

Query: 121 TSRGLEKEEKSASEAEGLGSLKDFNYARV------ER--EASEIEKEAWSLLRDSVVNYC 180
            + G+ K E+       +G  KD N+  V      ER    +E+EKEAW LLR +VVNYC
Sbjct: 147 KTEGIPKRERG-----NVGGGKDANFGNVGVRKETERCLSQTEVEKEAWKLLRGAVVNYC 206

Query: 181 GHPVGTVAANDPADTQPLNYDQVFVRDFVPSALAFLLNGEDEIVKNFLLHTLQLQSWEKT 240
           G PVGTVAANDP DTQ LNYDQVF+RDFVPSA AF+L+GE EIV+NFLLHTLQLQSWEKT
Sbjct: 207 GFPVGTVAANDPGDTQTLNYDQVFIRDFVPSAYAFMLDGEGEIVRNFLLHTLQLQSWEKT 266

Query: 241 VDCYSPGQGLMPASFKVRSQPLDGSDGAFEEVLDPDFGESAIGRVAPVDSGLWWIILLRA 300
           VDC+SPG GLMPASFKV+S PL+G+DG+FEE LDPDFG SAIGRV+PVDSGLWWIILLRA
Sbjct: 267 VDCHSPGPGLMPASFKVKSAPLEGNDGSFEEFLDPDFGGSAIGRVSPVDSGLWWIILLRA 326

Query: 301 YGKITGDYTLQERVDVQTGIRLILNLCLTNGFDMFPTLLVSDGSCMIDRRMGIHGHPLEI 360
           YGK+TGDYTLQER+DVQTGI+LIL LCL +GFDMFPTLLV+DGSCM+DRRMGIHGHPLEI
Sbjct: 327 YGKLTGDYTLQERIDVQTGIKLILKLCLADGFDMFPTLLVTDGSCMVDRRMGIHGHPLEI 386

Query: 361 QALFYSALRCSREMLIVNDATKNLVAALNSRLSSLSFHIREYYWIDMNKINEIYRYKTEE 420
           QALFYSALRC+REMLIVND TK+LV A+N+RLS+LSFHIREYYW+D+ KINEIYRY TEE
Sbjct: 387 QALFYSALRCAREMLIVNDGTKSLVTAVNNRLSALSFHIREYYWVDIKKINEIYRYNTEE 446

Query: 421 YSSEAVNKFNIYPEQIPSWLVDWIPEEGGYLIGNLQPAHMDFRFFTLGNLWSIVSSLGTP 480
           YS++A NKFNIYPEQIP+WLVDWIP++GGY IGNLQPAHMDFRFFTLGNLW+++SSLG  
Sbjct: 447 YSADATNKFNIYPEQIPTWLVDWIPDKGGYFIGNLQPAHMDFRFFTLGNLWAVISSLGNQ 506

Query: 481 KQNKGILNLIEAKWDDLVANMPLKICFPAMEYEEWRIVTGSDPKNTPWSYHNGGSWPTLL 540
           +QN+G++ LIE KWDDLVANMPLKICFPA+E +EWRI+TGSDPKNTPWSYHNGGSWPTLL
Sbjct: 507 EQNEGVMTLIEEKWDDLVANMPLKICFPALEKDEWRIITGSDPKNTPWSYHNGGSWPTLL 566

Query: 541 WQFTLACMKMGRPELARNAIAVAEKKLSADRWPEYYDMRSARLIGKQSRLFQTWTIAGFL 600
           WQFTLAC+KMG+ ELA+ A+AVAEK+L  D WPEYYD +S R +GKQSRL+QTWTIAGFL
Sbjct: 567 WQFTLACIKMGKLELAKKAVAVAEKRLKEDEWPEYYDTKSGRFVGKQSRLYQTWTIAGFL 626

Query: 601 TSKLLLENPEKASLLYWEEDYELLQSCVCALGKANG--NRCSRQRHP 623
            +K L+E PEKASLL+WEEDY+LL++CVC L K++G  N+CSR   P
Sbjct: 627 AAKKLIEQPEKASLLFWEEDYQLLETCVCGLSKSSGRKNKCSRFTPP 662

BLAST of MS008229 vs. ExPASy Swiss-Prot
Match: Q9FXA8 (Alkaline/neutral invertase A, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=INVA PE=1 SV=1)

HSP 1 Score: 870.5 bits (2248), Expect = 1.1e-251
Identity = 423/599 (70.62%), Postives = 484/599 (80.80%), Query Frame = 0

Query: 22  PNCSRIIGSVQVNDNSSRRGFGFSNGYRLSRGSGGKNRHLIANVASDFRNQSTSAEAHVN 81
           P+ SR   +  +   SS + F  S+ Y   +     NR          R  STS E +++
Sbjct: 33  PDLSR---TTSIRHLSSSQRFVSSSIYCFPQSKILPNRFSEKTTGISVRQFSTSVETNLS 92

Query: 82  QKSFETIYIQGGFTVKPLVIESIETSRGLEKEEKSASEAE--GLGSLKDFNYARVEREAS 141
            KSFE I++Q              +   LE+  K+  E E   +GS K      V RE S
Sbjct: 93  DKSFERIHVQ--------------SDAILERIHKNEEEVETVSIGSEK------VVREES 152

Query: 142 EIEKEAWSLLRDSVVNYCGHPVGTVAANDPADTQPLNYDQVFVRDFVPSALAFLLNGEDE 201
           E EKEAW +L ++VV YCG PVGTVAANDP D  PLNYDQVF+RDFVPSALAFLL GE +
Sbjct: 153 EAEKEAWRILENAVVRYCGSPVGTVAANDPGDKMPLNYDQVFIRDFVPSALAFLLKGEGD 212

Query: 202 IVKNFLLHTLQLQSWEKTVDCYSPGQGLMPASFKVRSQPLDGSDGAFEEVLDPDFGESAI 261
           IV+NFLLHTLQLQSWEKTVDCYSPGQGLMPASFKVR+  LD  +   EEVLDPDFGESAI
Sbjct: 213 IVRNFLLHTLQLQSWEKTVDCYSPGQGLMPASFKVRTVALD--ENTTEEVLDPDFGESAI 272

Query: 262 GRVAPVDSGLWWIILLRAYGKITGDYTLQERVDVQTGIRLILNLCLTNGFDMFPTLLVSD 321
           GRVAPVDSGLWWIILLRAYGKITGD++LQER+DVQTGI+LI+NLCL +GFDMFPTLLV+D
Sbjct: 273 GRVAPVDSGLWWIILLRAYGKITGDFSLQERIDVQTGIKLIMNLCLADGFDMFPTLLVTD 332

Query: 322 GSCMIDRRMGIHGHPLEIQALFYSALRCSREMLIVNDATKNLVAALNSRLSSLSFHIREY 381
           GSCMIDRRMGIHGHPLEIQ+LFYSALRCSREML VND++K+LV A+N+RLS+LSFHIREY
Sbjct: 333 GSCMIDRRMGIHGHPLEIQSLFYSALRCSREMLSVNDSSKDLVRAINNRLSALSFHIREY 392

Query: 382 YWIDMNKINEIYRYKTEEYSSEAVNKFNIYPEQIPSWLVDWIPEEGGYLIGNLQPAHMDF 441
           YW+D+ KINEIYRYKTEEYS++A NKFNIYPEQIP WL+DWIPE+GGYL+GNLQPAHMDF
Sbjct: 393 YWVDIKKINEIYRYKTEEYSTDATNKFNIYPEQIPPWLMDWIPEQGGYLLGNLQPAHMDF 452

Query: 442 RFFTLGNLWSIVSSLGTPKQNKGILNLIEAKWDDLVANMPLKICFPAMEYEEWRIVTGSD 501
           RFFTLGN WSIVSSL TPKQN+ ILNLIEAKWDD++ NMPLKIC+PA+EY++WRI+TGSD
Sbjct: 453 RFFTLGNFWSIVSSLATPKQNEAILNLIEAKWDDIIGNMPLKICYPALEYDDWRIITGSD 512

Query: 502 PKNTPWSYHNGGSWPTLLWQFTLACMKMGRPELARNAIAVAEKKLSADRWPEYYDMRSAR 561
           PKNTPWSYHN GSWPTLLWQFTLACMKMGRPELA  A+AVAEK+L ADRWPEYYD RS +
Sbjct: 513 PKNTPWSYHNSGSWPTLLWQFTLACMKMGRPELAEKALAVAEKRLLADRWPEYYDTRSGK 572

Query: 562 LIGKQSRLFQTWTIAGFLTSKLLLENPEKASLLYWEEDYELLQSCVCALGKANGNRCSR 619
            IGKQSRL+QTWT+AGFLTSKLLL NPE ASLL+WEEDYELL  C C L K++  +CSR
Sbjct: 573 FIGKQSRLYQTWTVAGFLTSKLLLANPEMASLLFWEEDYELLDICACGLRKSDRKKCSR 606

BLAST of MS008229 vs. ExPASy Swiss-Prot
Match: Q84JL5 (Probable alkaline/neutral invertase A, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=INVH PE=2 SV=1)

HSP 1 Score: 849.7 bits (2194), Expect = 2.1e-245
Identity = 401/558 (71.86%), Postives = 467/558 (83.69%), Query Frame = 0

Query: 63  ANVASDFRNQSTSAEAHVNQKSFETIYIQGGFTVKPLVIESIETSRGLEKEEKSASEAEG 122
           A V S+ R+ S S     N  + + IY + G  VKPLV+E ++     EK+E++ +E E 
Sbjct: 77  AQVVSEARSHSASTTC-ANDTTLDQIYTKNGLNVKPLVVERLKRD---EKDEEAVNEDE- 136

Query: 123 LGSLKDFNYARVEREASEIEKEAWSLLRDSVVNYCGHPVGTVAANDPADTQPLNYDQVFV 182
              +K   +  V  + +++E+EAW LLRDS+V YC  PVGTVAA DP DT P NYDQVF+
Sbjct: 137 -EGVKRDGFEGV--KCNDVEEEAWRLLRDSIVTYCDSPVGTVAAKDPTDTTPSNYDQVFI 196

Query: 183 RDFVPSALAFLLNGEDEIVKNFLLHTLQLQSWEKTVDCYSPGQGLMPASFKVRSQPLDGS 242
           RDFVPSALAFLL GE EIV+NFLLHTLQLQSWEKTVDCYSPGQGLMPASFKVR+ PL+  
Sbjct: 197 RDFVPSALAFLLKGESEIVRNFLLHTLQLQSWEKTVDCYSPGQGLMPASFKVRTLPLE-- 256

Query: 243 DGAFEEVLDPDFGESAIGRVAPVDSGLWWIILLRAYGKITGDYTLQERVDVQTGIRLILN 302
           +  FEEVLDPDFGE+AIGRVAPVDSGLWWIILLRAYGKITGDY+LQER+DVQTGI++I N
Sbjct: 257 EDKFEEVLDPDFGEAAIGRVAPVDSGLWWIILLRAYGKITGDYSLQERIDVQTGIKMIAN 316

Query: 303 LCLTNGFDMFPTLLVSDGSCMIDRRMGIHGHPLEIQALFYSALRCSREMLIVNDATKNLV 362
           LCL +GFDMFPTLLV+DGSCMIDRRMGIHGHPLEIQALFYSALR SREM+ VND++KN++
Sbjct: 317 LCLADGFDMFPTLLVTDGSCMIDRRMGIHGHPLEIQALFYSALRSSREMITVNDSSKNII 376

Query: 363 AALNSRLSSLSFHIREYYWIDMNKINEIYRYKTEEYSSEAVNKFNIYPEQIPSWLVDWIP 422
             +++RLS+LSFHIRE YW+D NKINEIYRYKTEEYS +A NKFNIYPEQ+  WL+DW+P
Sbjct: 377 KTISNRLSALSFHIRENYWVDKNKINEIYRYKTEEYSMDATNKFNIYPEQVSPWLMDWVP 436

Query: 423 E--EGGYLIGNLQPAHMDFRFFTLGNLWSIVSSLGTPKQNKGILNLIEAKWDDLVANMPL 482
           E  + G+LIGNLQPAHMDFRFFTLGNLWSI+SSLGTPKQN+ ILNL+E KWDDLV +MPL
Sbjct: 437 ESPDSGFLIGNLQPAHMDFRFFTLGNLWSIISSLGTPKQNQAILNLVEEKWDDLVGHMPL 496

Query: 483 KICFPAMEYEEWRIVTGSDPKNTPWSYHNGGSWPTLLWQFTLACMKMGRPELARNAIAVA 542
           KIC+PA+E  EW I+TGSDPKNTPWSYHNGGSWPTLLWQFTLAC+KMGRPELA  A+ +A
Sbjct: 497 KICYPALESSEWHIITGSDPKNTPWSYHNGGSWPTLLWQFTLACIKMGRPELAEKAVTLA 556

Query: 543 EKKLSADRWPEYYDMRSARLIGKQSRLFQTWTIAGFLTSKLLLENPEKASLLYWEEDYEL 602
           EK+L ADRWPEYYD R  + IGKQSRL+QTWTIAGFLTSK LL+NPE AS L+WEED EL
Sbjct: 557 EKRLQADRWPEYYDTRDGKFIGKQSRLYQTWTIAGFLTSKQLLQNPEIASSLFWEEDLEL 616

Query: 603 LQSCVCALGKANGNRCSR 619
           L+SCVC L K+   +CSR
Sbjct: 617 LESCVCVLTKSGRKKCSR 624

BLAST of MS008229 vs. ExPASy Swiss-Prot
Match: Q10MC0 (Neutral/alkaline invertase 1, mitochondrial OS=Oryza sativa subsp. japonica OX=39947 GN=NIN1 PE=1 SV=1)

HSP 1 Score: 837.4 bits (2162), Expect = 1.1e-241
Identity = 393/510 (77.06%), Postives = 448/510 (87.84%), Query Frame = 0

Query: 111 EKEEKSASEAEGLGSLKDFNYARVEREASEIEKEAWSLLRDSVVNYCGHPVGTVAANDPA 170
           EK+  + +    L  LK +      R+ S  EKEAWSLL  SVV+YCG  VGTVAANDP+
Sbjct: 111 EKDPVATACQHELEGLKAWVETVRSRKESTEEKEAWSLLGRSVVSYCGTAVGTVAANDPS 170

Query: 171 D-TQPLNYDQVFVRDFVPSALAFLLNGEDEIVKNFLLHTLQLQSWEKTVDCYSPGQGLMP 230
              Q LNYDQVF+RDFVPSA+AFLL GE +IVKNFLLHTLQLQSWEKTVDCYSPGQGLMP
Sbjct: 171 TANQMLNYDQVFIRDFVPSAIAFLLKGEGDIVKNFLLHTLQLQSWEKTVDCYSPGQGLMP 230

Query: 231 ASFKVRSQPLDGSDGAFEEVLDPDFGESAIGRVAPVDSGLWWIILLRAYGKITGDYTLQE 290
           ASFKVRS PLDG+  AFEEVLDPDFGESAIGRVAPVDSGLWWIILLRAYGKITGDY LQE
Sbjct: 231 ASFKVRSIPLDGNSEAFEEVLDPDFGESAIGRVAPVDSGLWWIILLRAYGKITGDYALQE 290

Query: 291 RVDVQTGIRLILNLCLTNGFDMFPTLLVSDGSCMIDRRMGIHGHPLEIQALFYSALRCSR 350
           RVDVQTGIRLILNLCL++GFDMFPTLLV+DGSCMIDRRMGIHGHPLEIQ+LFYSALRC+R
Sbjct: 291 RVDVQTGIRLILNLCLSDGFDMFPTLLVTDGSCMIDRRMGIHGHPLEIQSLFYSALRCAR 350

Query: 351 EMLIVNDATKNLVAALNSRLSSLSFHIREYYWIDMNKINEIYRYKTEEYSSEAVNKFNIY 410
           EM+ VND + +L+ A+N RLS+LSFHIREYYW+DM KINEIYRYKTEEYS +A+NKFNIY
Sbjct: 351 EMVSVNDGSNSLIRAINYRLSALSFHIREYYWVDMKKINEIYRYKTEEYSHDAINKFNIY 410

Query: 411 PEQIPSWLVDWIPEEGGYLIGNLQPAHMDFRFFTLGNLWSIVSSLGTPKQNKGILNLIEA 470
           PEQIPSWL DWIPE+GGYLIGNLQPAHMDFRFF+LGNLW+I+SSL T +Q +GILNLIEA
Sbjct: 411 PEQIPSWLADWIPEKGGYLIGNLQPAHMDFRFFSLGNLWAIISSLATQRQAEGILNLIEA 470

Query: 471 KWDDLVANMPLKICFPAMEYEEWRIVTGSDPKNTPWSYHNGGSWPTLLWQFTLACMKMGR 530
           KW+D++ANMPLKIC+PA+EYEEWRI+TGSDPKNTPWSYHNGGSWPTLLWQFTLAC+KMGR
Sbjct: 471 KWEDIIANMPLKICYPALEYEEWRIITGSDPKNTPWSYHNGGSWPTLLWQFTLACIKMGR 530

Query: 531 PELARNAIAVAEKKLSADRWPEYYDMRSARLIGKQSRLFQTWTIAGFLTSKLLLENPEKA 590
            +LA+ AI VAEK+LS D+WPEYYD R+ R IGKQSRL+QTWTIAG+L+SK+LL+ PE A
Sbjct: 531 RDLAQRAIEVAEKRLSEDKWPEYYDTRTGRFIGKQSRLYQTWTIAGYLSSKMLLDCPELA 590

Query: 591 SLLYWEEDYELLQSCVCALGKANGNRCSRQ 620
           S+L  EED ELL+ C C++ K+   +CSR+
Sbjct: 591 SILICEEDLELLEGCACSVNKSARTKCSRR 620

BLAST of MS008229 vs. ExPASy Swiss-Prot
Match: Q6H6N5 (Neutral/alkaline invertase 3, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=NIN3 PE=2 SV=1)

HSP 1 Score: 763.8 bits (1971), Expect = 1.5e-219
Identity = 344/468 (73.50%), Postives = 408/468 (87.18%), Query Frame = 0

Query: 136 REASEIEKEAWSLLRDSVVNYCGHPVGTVAANDPADTQPLNYDQVFVRDFVPSALAFLLN 195
           R+AS +E EAW LLR+SVV YCG PVGT+AANDP D  P+NYDQVF+RDF+PS +AFLL 
Sbjct: 121 RKASSVEDEAWELLRESVVYYCGSPVGTIAANDPNDANPMNYDQVFIRDFIPSGIAFLLK 180

Query: 196 GEDEIVKNFLLHTLQLQSWEKTVDCYSPGQGLMPASFKVRSQPLDGSDGAFEEVLDPDFG 255
           GE EIV+NF+LHTLQLQSWEKT+DC+SPGQGLMPASFKVR+ PLDG + A EEVLDPDFG
Sbjct: 181 GEYEIVRNFILHTLQLQSWEKTMDCHSPGQGLMPASFKVRTIPLDGDEDATEEVLDPDFG 240

Query: 256 ESAIGRVAPVDSGLWWIILLRAYGKITGDYTLQERVDVQTGIRLILNLCLTNGFDMFPTL 315
           E+AIGRVAPVDSGLWWIILLRAYGK +GD T+QER+DVQTGI++IL LCL +GFDMFPTL
Sbjct: 241 EAAIGRVAPVDSGLWWIILLRAYGKCSGDLTVQERIDVQTGIKMILKLCLADGFDMFPTL 300

Query: 316 LVSDGSCMIDRRMGIHGHPLEIQALFYSALRCSREMLIVNDATKNLVAALNSRLSSLSFH 375
           LV+DGSCMIDRRMGIHGHPLEIQALFYSAL C+REML   D + +L+ ALN+RL +LSFH
Sbjct: 301 LVTDGSCMIDRRMGIHGHPLEIQALFYSALLCAREMLTPEDGSADLIRALNNRLIALSFH 360

Query: 376 IREYYWIDMNKINEIYRYKTEEYSSEAVNKFNIYPEQIPSWLVDWIPEEGGYLIGNLQPA 435
           IREYYW+DM K+NEIYRYKTEEYS +AVNKFNIYP+Q+  WLV+WIP +GGY IGNLQPA
Sbjct: 361 IREYYWVDMQKLNEIYRYKTEEYSYDAVNKFNIYPDQVSPWLVEWIPPKGGYFIGNLQPA 420

Query: 436 HMDFRFFTLGNLWSIVSSLGTPKQNKGILNLIEAKWDDLVANMPLKICFPAMEYEEWRIV 495
           HMDFRFF+LGNLWSIVSSL T  Q+  IL+LIE+KW DLVA MPLKIC+PA+E +EW+I+
Sbjct: 421 HMDFRFFSLGNLWSIVSSLATTHQSHAILDLIESKWSDLVAEMPLKICYPALENQEWKII 480

Query: 496 TGSDPKNTPWSYHNGGSWPTLLWQFTLACMKMGRPELARNAIAVAEKKLSADRWPEYYDM 555
           TGSDPKNTPWSYHNGGSWPTLLWQ T+A +KM RPE+A  A+ VAE++++ D+WPEYYD 
Sbjct: 481 TGSDPKNTPWSYHNGGSWPTLLWQLTVASIKMNRPEIAAKAVEVAERRIAIDKWPEYYDT 540

Query: 556 RSARLIGKQSRLFQTWTIAGFLTSKLLLENPEKASLLYWEEDYELLQS 604
           + AR IGKQSRL+QTW+IAG+L +K LL+ P+ A +L  +ED E+L +
Sbjct: 541 KRARFIGKQSRLYQTWSIAGYLVAKQLLDKPDAARILSNDEDSEILNA 588

BLAST of MS008229 vs. ExPASy TrEMBL
Match: A0A6J1C8V3 (Alkaline/neutral invertase OS=Momordica charantia OX=3673 GN=LOC111009384 PE=3 SV=1)

HSP 1 Score: 1297.0 bits (3355), Expect = 0.0e+00
Identity = 629/630 (99.84%), Postives = 630/630 (100.00%), Query Frame = 0

Query: 1   MFGASPPRPKYRRIDKISKLRPNCSRIIGSVQVNDNSSRRGFGFSNGYRLSRGSGGKNRH 60
           MFGASPPRPKYRRIDKISKLRPNCSRIIGSVQVNDNSSRRGFGFSNGYRLSRGSGGKNRH
Sbjct: 26  MFGASPPRPKYRRIDKISKLRPNCSRIIGSVQVNDNSSRRGFGFSNGYRLSRGSGGKNRH 85

Query: 61  LIANVASDFRNQSTSAEAHVNQKSFETIYIQGGFTVKPLVIESIETSRGLEKEEKSASEA 120
           LIANVASDFRNQSTSAEAHVNQKSFETIYIQGGFTVKPLVIESIET+RGLEKEEKSASEA
Sbjct: 86  LIANVASDFRNQSTSAEAHVNQKSFETIYIQGGFTVKPLVIESIETNRGLEKEEKSASEA 145

Query: 121 EGLGSLKDFNYARVEREASEIEKEAWSLLRDSVVNYCGHPVGTVAANDPADTQPLNYDQV 180
           EGLGSLKDFNYARVEREASEIEKEAWSLLRDSVVNYCGHPVGTVAANDPADTQPLNYDQV
Sbjct: 146 EGLGSLKDFNYARVEREASEIEKEAWSLLRDSVVNYCGHPVGTVAANDPADTQPLNYDQV 205

Query: 181 FVRDFVPSALAFLLNGEDEIVKNFLLHTLQLQSWEKTVDCYSPGQGLMPASFKVRSQPLD 240
           FVRDFVPSALAFLLNGEDEIVKNFLLHTLQLQSWEKTVDCYSPGQGLMPASFKVRSQPLD
Sbjct: 206 FVRDFVPSALAFLLNGEDEIVKNFLLHTLQLQSWEKTVDCYSPGQGLMPASFKVRSQPLD 265

Query: 241 GSDGAFEEVLDPDFGESAIGRVAPVDSGLWWIILLRAYGKITGDYTLQERVDVQTGIRLI 300
           GSDGAFEEVLDPDFGESAIGRVAPVDSGLWWIILLRAYGKITGDYTLQERVDVQTGIRLI
Sbjct: 266 GSDGAFEEVLDPDFGESAIGRVAPVDSGLWWIILLRAYGKITGDYTLQERVDVQTGIRLI 325

Query: 301 LNLCLTNGFDMFPTLLVSDGSCMIDRRMGIHGHPLEIQALFYSALRCSREMLIVNDATKN 360
           LNLCLTNGFDMFPTLLVSDGSCMIDRRMGIHGHPLEIQALFYSALRCSREMLIVNDATKN
Sbjct: 326 LNLCLTNGFDMFPTLLVSDGSCMIDRRMGIHGHPLEIQALFYSALRCSREMLIVNDATKN 385

Query: 361 LVAALNSRLSSLSFHIREYYWIDMNKINEIYRYKTEEYSSEAVNKFNIYPEQIPSWLVDW 420
           LVAALNSRLSSLSFHIREYYWIDMNKINEIYRYKTEEYSSEAVNKFNIYPEQIPSWLVDW
Sbjct: 386 LVAALNSRLSSLSFHIREYYWIDMNKINEIYRYKTEEYSSEAVNKFNIYPEQIPSWLVDW 445

Query: 421 IPEEGGYLIGNLQPAHMDFRFFTLGNLWSIVSSLGTPKQNKGILNLIEAKWDDLVANMPL 480
           IPEEGGYLIGNLQPAHMDFRFFTLGNLWSIVSSLGTPKQNKGILNLIEAKWDDLVANMPL
Sbjct: 446 IPEEGGYLIGNLQPAHMDFRFFTLGNLWSIVSSLGTPKQNKGILNLIEAKWDDLVANMPL 505

Query: 481 KICFPAMEYEEWRIVTGSDPKNTPWSYHNGGSWPTLLWQFTLACMKMGRPELARNAIAVA 540
           KICFPAMEYEEWRIVTGSDPKNTPWSYHNGGSWPTLLWQFTLACMKMGRPELARNAIAVA
Sbjct: 506 KICFPAMEYEEWRIVTGSDPKNTPWSYHNGGSWPTLLWQFTLACMKMGRPELARNAIAVA 565

Query: 541 EKKLSADRWPEYYDMRSARLIGKQSRLFQTWTIAGFLTSKLLLENPEKASLLYWEEDYEL 600
           EKKLSADRWPEYYDMRSARLIGKQSRLFQTWTIAGFLTSKLLLENPEKASLLYWEEDYEL
Sbjct: 566 EKKLSADRWPEYYDMRSARLIGKQSRLFQTWTIAGFLTSKLLLENPEKASLLYWEEDYEL 625

Query: 601 LQSCVCALGKANGNRCSRQRHPARPDDHDH 631
           LQSCVCALGKANGNRCSRQRHPARPDDHDH
Sbjct: 626 LQSCVCALGKANGNRCSRQRHPARPDDHDH 655

BLAST of MS008229 vs. ExPASy TrEMBL
Match: A0A1S3AX88 (Alkaline/neutral invertase OS=Cucumis melo OX=3656 GN=LOC103483905 PE=3 SV=1)

HSP 1 Score: 1143.3 bits (2956), Expect = 0.0e+00
Identity = 550/631 (87.16%), Postives = 584/631 (92.55%), Query Frame = 0

Query: 1   MFGA-SPPRPKYRRIDKISKLRPNCSRIIGSVQVNDNSSRRGFGFSNGYRLSRGSGGKNR 60
           MFG  + P+ KY+RI + SKL PN  ++IGSVQV D  SRR F FSNGYRL +GS  +NR
Sbjct: 26  MFGTIASPKLKYKRIGRFSKLEPNGCKVIGSVQVVDKLSRRCFCFSNGYRLYKGSSDRNR 85

Query: 61  HLIANVASDFRNQSTSAEAHVNQKSFETIYIQGGFTVKPLVIESIETSRGLEKEEKSASE 120
           HLIANVASDFRNQSTSAE++V QKSF+ IYI GGF VKPL IESIET   + KE+K  SE
Sbjct: 86  HLIANVASDFRNQSTSAESYVKQKSFDAIYINGGFKVKPLEIESIETGHDIVKEDKKVSE 145

Query: 121 AEGLGSLKDFNYARVEREASEIEKEAWSLLRDSVVNYCGHPVGTVAANDPADTQPLNYDQ 180
            EGLGSLK  NY+RVERE S+IEKEAW LLR+SVV YCGHPVGTVAANDPAD QPLNYDQ
Sbjct: 146 VEGLGSLKGSNYSRVERELSKIEKEAWDLLRNSVVFYCGHPVGTVAANDPADAQPLNYDQ 205

Query: 181 VFVRDFVPSALAFLLNGEDEIVKNFLLHTLQLQSWEKTVDCYSPGQGLMPASFKVRSQPL 240
           VFVRDFVPSALAFLLNGE+EIVKNFLLHTLQLQSWEKTVDCYSPGQGLMPASFKVRSQPL
Sbjct: 206 VFVRDFVPSALAFLLNGEEEIVKNFLLHTLQLQSWEKTVDCYSPGQGLMPASFKVRSQPL 265

Query: 241 DGSDGAFEEVLDPDFGESAIGRVAPVDSGLWWIILLRAYGKITGDYTLQERVDVQTGIRL 300
           DGSDGAFEEVLDPDFGESAIGRVAPVDSGLWWIILLRAYGKITGDYTLQERVDVQTGIRL
Sbjct: 266 DGSDGAFEEVLDPDFGESAIGRVAPVDSGLWWIILLRAYGKITGDYTLQERVDVQTGIRL 325

Query: 301 ILNLCLTNGFDMFPTLLVSDGSCMIDRRMGIHGHPLEIQALFYSALRCSREMLIVNDATK 360
           ILNLCLTNGFDMFPTLLVSDGSCMIDRRMGIHGHPLEIQALFYSALRCSREMLIVND+TK
Sbjct: 326 ILNLCLTNGFDMFPTLLVSDGSCMIDRRMGIHGHPLEIQALFYSALRCSREMLIVNDSTK 385

Query: 361 NLVAALNSRLSSLSFHIREYYWIDMNKINEIYRYKTEEYSSEAVNKFNIYPEQIPSWLVD 420
           NLV  LN+RLS+LSFHIREYYW+D NKINEIYRYKTEEYS++AVNKFNIYPEQIPSWLVD
Sbjct: 386 NLVVELNNRLSALSFHIREYYWVDKNKINEIYRYKTEEYSTDAVNKFNIYPEQIPSWLVD 445

Query: 421 WIPEEGGYLIGNLQPAHMDFRFFTLGNLWSIVSSLGTPKQNKGILNLIEAKWDDLVANMP 480
           WIPEEGGY IGNLQPAHMDFRFFTLGNLWS+VSSLGTPKQN+GILNLIEAKWDDLVANMP
Sbjct: 446 WIPEEGGYFIGNLQPAHMDFRFFTLGNLWSVVSSLGTPKQNEGILNLIEAKWDDLVANMP 505

Query: 481 LKICFPAMEYEEWRIVTGSDPKNTPWSYHNGGSWPTLLWQFTLACMKMGRPELARNAIAV 540
           LKICFPAMEYEEWRI+TGSDPKNTPWSYHNGGSWPTLLWQFTLAC+KMGRPELARNAIAV
Sbjct: 506 LKICFPAMEYEEWRIITGSDPKNTPWSYHNGGSWPTLLWQFTLACIKMGRPELARNAIAV 565

Query: 541 AEKKLSADRWPEYYDMRSARLIGKQSRLFQTWTIAGFLTSKLLLENPEKASLLYWEEDYE 600
           AEKKLS DRWPEYYDMRSARLIGKQSRLFQTWTIAGFLTSKLLLENPEKASLL+WEEDYE
Sbjct: 566 AEKKLSVDRWPEYYDMRSARLIGKQSRLFQTWTIAGFLTSKLLLENPEKASLLFWEEDYE 625

Query: 601 LLQSCVCALGKANGNRCSRQRHPARPDDHDH 631
           +LQ+CVCALGK NGN+CSR R   +P + +H
Sbjct: 626 ILQNCVCALGKGNGNKCSRHRQHPKPSNPNH 656

BLAST of MS008229 vs. ExPASy TrEMBL
Match: A0A0A0L968 (Alkaline/neutral invertase OS=Cucumis sativus OX=3659 GN=Csa_3G168930 PE=3 SV=1)

HSP 1 Score: 1130.9 bits (2924), Expect = 0.0e+00
Identity = 543/626 (86.74%), Postives = 580/626 (92.65%), Query Frame = 0

Query: 1   MFGA-SPPRPKYRRIDKISKLRPNCSRIIGSVQVNDNSSRRGFGFSNGYRLSRGSGGKNR 60
           MFG  + P+ KY+RI + SKL PN  +I GSVQV DN SRR   FSNGYRL +GS  +NR
Sbjct: 26  MFGTIASPKLKYKRIGRFSKLEPNGCKITGSVQVVDNLSRRCICFSNGYRLYKGSNDRNR 85

Query: 61  HLIANVASDFRNQSTSAEAHVNQKSFETIYIQGGFTVKPLVIESIETSRGLEKEEKSASE 120
            LIANVASDFRNQSTS+E++V QKSF+TIYI GGF VKPL IESIET   + KE+K  SE
Sbjct: 86  CLIANVASDFRNQSTSSESYVKQKSFDTIYINGGFKVKPLEIESIETGHDIVKEDKKVSE 145

Query: 121 AEGLGSLKDFNYARVEREASEIEKEAWSLLRDSVVNYCGHPVGTVAANDPADTQPLNYDQ 180
            EGLGSLK  NY+RVERE S+IEKEAW LLR+SVV YCGHPVGTVAANDPAD+QPLNYDQ
Sbjct: 146 VEGLGSLKGSNYSRVEREVSKIEKEAWDLLRNSVVFYCGHPVGTVAANDPADSQPLNYDQ 205

Query: 181 VFVRDFVPSALAFLLNGEDEIVKNFLLHTLQLQSWEKTVDCYSPGQGLMPASFKVRSQPL 240
           VFVRDF+PSALAFLLNGE+EIVKNFLLHTLQLQSWEKTVDCYSPGQGLMPASFKVRSQPL
Sbjct: 206 VFVRDFIPSALAFLLNGEEEIVKNFLLHTLQLQSWEKTVDCYSPGQGLMPASFKVRSQPL 265

Query: 241 DGSDGAFEEVLDPDFGESAIGRVAPVDSGLWWIILLRAYGKITGDYTLQERVDVQTGIRL 300
           DGSDGAFEEVLDPDFGESAIGRVAPVDSGLWWIIL+RAYGKITGDYTLQERVDVQTGIRL
Sbjct: 266 DGSDGAFEEVLDPDFGESAIGRVAPVDSGLWWIILVRAYGKITGDYTLQERVDVQTGIRL 325

Query: 301 ILNLCLTNGFDMFPTLLVSDGSCMIDRRMGIHGHPLEIQALFYSALRCSREMLIVNDATK 360
           ILNLCLTNGFDMFPTLLVSDGSCMIDRRMGIHGHPLEIQALFYSALRCSREMLIVND+TK
Sbjct: 326 ILNLCLTNGFDMFPTLLVSDGSCMIDRRMGIHGHPLEIQALFYSALRCSREMLIVNDSTK 385

Query: 361 NLVAALNSRLSSLSFHIREYYWIDMNKINEIYRYKTEEYSSEAVNKFNIYPEQIPSWLVD 420
           NLV  LN+RLS+LSFHIREYYW+D NKINEIYRYKTEEYSS+AVNKFNIYPEQIPSWLVD
Sbjct: 386 NLVVELNNRLSALSFHIREYYWVDKNKINEIYRYKTEEYSSDAVNKFNIYPEQIPSWLVD 445

Query: 421 WIPEEGGYLIGNLQPAHMDFRFFTLGNLWSIVSSLGTPKQNKGILNLIEAKWDDLVANMP 480
           WIPEEGGY +GNLQPAHMDFRFFTLGNLWSIVSSLGTPKQN+GILNLIEAKWDDLVANMP
Sbjct: 446 WIPEEGGYFMGNLQPAHMDFRFFTLGNLWSIVSSLGTPKQNEGILNLIEAKWDDLVANMP 505

Query: 481 LKICFPAMEYEEWRIVTGSDPKNTPWSYHNGGSWPTLLWQFTLACMKMGRPELARNAIAV 540
           LKICFPAMEYEEWRI+TGSDPKNTPWSYHNGGSWPTLLWQFTLAC+KMGRPE+ARNAIAV
Sbjct: 506 LKICFPAMEYEEWRIITGSDPKNTPWSYHNGGSWPTLLWQFTLACIKMGRPEVARNAIAV 565

Query: 541 AEKKLSADRWPEYYDMRSARLIGKQSRLFQTWTIAGFLTSKLLLENPEKASLLYWEEDYE 600
           AEKKLS DRWPEYYDMRSARLIGKQSRLFQTWTIAGFLTSKLLLENPEKASLL+WEEDY+
Sbjct: 566 AEKKLSIDRWPEYYDMRSARLIGKQSRLFQTWTIAGFLTSKLLLENPEKASLLFWEEDYD 625

Query: 601 LLQSCVCALGKANGNRCSRQRHPARP 626
           +LQ+C+CAL K NGN+CSR R   +P
Sbjct: 626 ILQNCICALSKGNGNKCSRHRQHPKP 651

BLAST of MS008229 vs. ExPASy TrEMBL
Match: A0A6J1GVX4 (Alkaline/neutral invertase OS=Cucurbita moschata OX=3662 GN=LOC111457912 PE=3 SV=1)

HSP 1 Score: 1110.5 bits (2871), Expect = 0.0e+00
Identity = 544/633 (85.94%), Postives = 581/633 (91.79%), Query Frame = 0

Query: 1   MFG-ASPPRPKYRRIDKISKLRPNCSRIIGSVQVNDNSSRRGFGFSNGYRLSRGSGGKNR 60
           MFG  S  + KYRRI + SKL P+  +IIGSVQV  + +RR F  SN +RL +G+ G+NR
Sbjct: 26  MFGTTSLAKAKYRRIGRFSKLEPSGRKIIGSVQVVGDLNRRCFSCSNLHRLYKGNSGRNR 85

Query: 61  HLIANVASDFRNQSTSAEAHVNQKSFETIYIQGGFTVKPLVIESIETSRGLEKEEKSASE 120
            LIANVASDFRNQSTSAE HV QKSFE IYIQGGF VKPLVIESIET   L K+EK  SE
Sbjct: 86  CLIANVASDFRNQSTSAEPHVKQKSFERIYIQGGFKVKPLVIESIETD--LVKDEKKVSE 145

Query: 121 AEGLGSLKDFNYARVEREASEIEKEAWSLLRDSVVNYCGHPVGTVAANDPADTQPLNYDQ 180
            E L  LK    +RVERE S+IEKEAW+LLRDSVVNYCGHPVGTVAANDP+DTQPLNYDQ
Sbjct: 146 VEELSGLKG---SRVEREVSKIEKEAWNLLRDSVVNYCGHPVGTVAANDPSDTQPLNYDQ 205

Query: 181 VFVRDFVPSALAFLLNGEDEIVKNFLLHTLQLQSWEKTVDCYSPGQGLMPASFKVRSQPL 240
           VFVRDFVPSALAFLLNGE+EIVKNFLLHTLQLQSWEKTVDCYSPGQGLMPASFKVRSQPL
Sbjct: 206 VFVRDFVPSALAFLLNGEEEIVKNFLLHTLQLQSWEKTVDCYSPGQGLMPASFKVRSQPL 265

Query: 241 DGSDGAFEEVLDPDFGESAIGRVAPVDSGLWWIILLRAYGKITGDYTLQERVDVQTGIRL 300
           DGSDGAFEEVLDPDFGESAIGRVAPVDSGLWWIILLRAYGKITGDYTLQER+DVQTGIRL
Sbjct: 266 DGSDGAFEEVLDPDFGESAIGRVAPVDSGLWWIILLRAYGKITGDYTLQERIDVQTGIRL 325

Query: 301 ILNLCLTNGFDMFPTLLVSDGSCMIDRRMGIHGHPLEIQALFYSALRCSREMLIVNDATK 360
           ILNLCLTNGFDMFPTLLV DGSCMIDRRMGIHGHPLEIQALFYSALRCSREMLIVND+TK
Sbjct: 326 ILNLCLTNGFDMFPTLLVGDGSCMIDRRMGIHGHPLEIQALFYSALRCSREMLIVNDSTK 385

Query: 361 NLVAALNSRLSSLSFHIREYYWIDMNKINEIYRYKTEEYSSEAVNKFNIYPEQIPSWLVD 420
           NLVAALN+RLS+LSFHIREY+W+D NK+NEIYRY+TEEYS++AVNKFNIYPEQIP WLVD
Sbjct: 386 NLVAALNNRLSALSFHIREYFWVDKNKLNEIYRYRTEEYSTDAVNKFNIYPEQIPGWLVD 445

Query: 421 WIPEEGGYLIGNLQPAHMDFRFFTLGNLWSIVSSLGTPKQNKGILNLIEAKWDDLVANMP 480
           WIPEEGGYLIGNLQPAHMDFRFFTLGNLWSIVSSLGTP+QN+GILNLIEAKWDDLVANMP
Sbjct: 446 WIPEEGGYLIGNLQPAHMDFRFFTLGNLWSIVSSLGTPQQNEGILNLIEAKWDDLVANMP 505

Query: 481 LKICFPAMEYEEWRIVTGSDPKNTPWSYHNGGSWPTLLWQFTLACMKMGRPELARNAIAV 540
           LKICFPAME+EEWRI+TGSDPKNTPWSYHNGGSWPTLLWQFTLACMKMGRPELAR AIAV
Sbjct: 506 LKICFPAMEHEEWRIITGSDPKNTPWSYHNGGSWPTLLWQFTLACMKMGRPELARKAIAV 565

Query: 541 AEKKLSADRWPEYYDMRSARLIGKQSRLFQTWTIAGFLTSKLLLENPEKASLLYWEEDYE 600
           AEKKLSADRWPEYYDMRSA LIGKQSRLFQTWTIAGFLTSKLLL+NPEKASLL+WEEDYE
Sbjct: 566 AEKKLSADRWPEYYDMRSASLIGKQSRLFQTWTIAGFLTSKLLLDNPEKASLLFWEEDYE 625

Query: 601 LLQSCVCALGKANGNRCSRQRH--PARPDDHDH 631
           LLQ CVC LGKANGN+CSR RH    +P++ +H
Sbjct: 626 LLQGCVCVLGKANGNKCSRHRHRQHRKPNNLNH 653

BLAST of MS008229 vs. ExPASy TrEMBL
Match: A0A6J1IXE1 (Alkaline/neutral invertase OS=Cucurbita maxima OX=3661 GN=LOC111479350 PE=3 SV=1)

HSP 1 Score: 1099.3 bits (2842), Expect = 0.0e+00
Identity = 541/633 (85.47%), Postives = 575/633 (90.84%), Query Frame = 0

Query: 1   MFG-ASPPRPKYRRIDKISKLRPNCSRIIGSVQVNDNSSRRGFGFSNGYRLSRGSGGKNR 60
           MFG  S P+ K RRI + SKL P+  +I+GSVQV  + +RR F  SN YRL +G+ G+NR
Sbjct: 26  MFGTTSLPKAKCRRIGRFSKLEPSGRKIVGSVQVVGDLNRRCFSCSNLYRLYKGNSGRNR 85

Query: 61  HLIANVASDFRNQSTSAEAHVNQKSFETIYIQGGFTVKPLVIESIETSRGLEKEEKSASE 120
            LIANVASDFRNQSTSAE HV QKSFE IYIQGGF VKPLVIESIET   L K+EK  SE
Sbjct: 86  CLIANVASDFRNQSTSAEPHVKQKSFERIYIQGGFKVKPLVIESIETD--LVKDEKKVSE 145

Query: 121 AEGLGSLKDFNYARVEREASEIEKEAWSLLRDSVVNYCGHPVGTVAANDPADTQPLNYDQ 180
            E L  LK    +RVERE S+IEKEAW LLRDSVVNYCGHPVGTVAANDP+DTQPLNYDQ
Sbjct: 146 VEELSGLKG---SRVEREVSKIEKEAWILLRDSVVNYCGHPVGTVAANDPSDTQPLNYDQ 205

Query: 181 VFVRDFVPSALAFLLNGEDEIVKNFLLHTLQLQSWEKTVDCYSPGQGLMPASFKVRSQPL 240
           VFVRDFVPSALAFLLNGE EIVKNFLLHTLQLQSWEKTVDCYSPGQGLMPASFKVRSQPL
Sbjct: 206 VFVRDFVPSALAFLLNGEQEIVKNFLLHTLQLQSWEKTVDCYSPGQGLMPASFKVRSQPL 265

Query: 241 DGSDGAFEEVLDPDFGESAIGRVAPVDSGLWWIILLRAYGKITGDYTLQERVDVQTGIRL 300
           DG+DGAFEEVLDPDFGESAIGRVAPVDSGLWWIILLRAYGKITGDYTLQERVDVQTGIRL
Sbjct: 266 DGNDGAFEEVLDPDFGESAIGRVAPVDSGLWWIILLRAYGKITGDYTLQERVDVQTGIRL 325

Query: 301 ILNLCLTNGFDMFPTLLVSDGSCMIDRRMGIHGHPLEIQALFYSALRCSREMLIVNDATK 360
           ILNLCLTNGFDMFPTLLV DGSCMIDRRMGIHGHPLEIQALFYSALRCSREMLIVND+TK
Sbjct: 326 ILNLCLTNGFDMFPTLLVGDGSCMIDRRMGIHGHPLEIQALFYSALRCSREMLIVNDSTK 385

Query: 361 NLVAALNSRLSSLSFHIREYYWIDMNKINEIYRYKTEEYSSEAVNKFNIYPEQIPSWLVD 420
           NLVAALN+RLS+LSFHIREY+W+D NK+NEIYRY+TEEYS++AVNKFNIYPEQIP WLVD
Sbjct: 386 NLVAALNNRLSALSFHIREYFWVDKNKLNEIYRYRTEEYSTDAVNKFNIYPEQIPGWLVD 445

Query: 421 WIPEEGGYLIGNLQPAHMDFRFFTLGNLWSIVSSLGTPKQNKGILNLIEAKWDDLVANMP 480
           WIPEE GYLIGNLQ AHMDFRFFTLGNLWSIVSSLGTP+QN+GILNLIEAKWDDLVANMP
Sbjct: 446 WIPEESGYLIGNLQSAHMDFRFFTLGNLWSIVSSLGTPQQNEGILNLIEAKWDDLVANMP 505

Query: 481 LKICFPAMEYEEWRIVTGSDPKNTPWSYHNGGSWPTLLWQFTLACMKMGRPELARNAIAV 540
           LKICFPAME+EEWRI+TGSDPKNTPWSYHNGGSWPTLLWQFTLACMKMGRPELAR AIAV
Sbjct: 506 LKICFPAMEHEEWRIITGSDPKNTPWSYHNGGSWPTLLWQFTLACMKMGRPELARKAIAV 565

Query: 541 AEKKLSADRWPEYYDMRSARLIGKQSRLFQTWTIAGFLTSKLLLENPEKASLLYWEEDYE 600
           AEKKLSADRWPEYYDMRSA LIGKQSRLFQTWTIAGFLTSKLLLENPEKASLL+WEEDYE
Sbjct: 566 AEKKLSADRWPEYYDMRSASLIGKQSRLFQTWTIAGFLTSKLLLENPEKASLLFWEEDYE 625

Query: 601 LLQSCVCALGKANGNRCSRQR--HPARPDDHDH 631
           LLQ CVC LG ANGN+CSR R     +P++ +H
Sbjct: 626 LLQGCVCVLGTANGNKCSRHRPGQHRKPNNLNH 653

BLAST of MS008229 vs. TAIR 10
Match: AT3G06500.1 (Plant neutral invertase family protein )

HSP 1 Score: 899.0 bits (2322), Expect = 2.1e-261
Identity = 439/647 (67.85%), Postives = 522/647 (80.68%), Query Frame = 0

Query: 1   MFGASPP---------RPKYRRIDKIS-KLRPNCSRII--GSVQVNDNSSRRGFGFSNGY 60
           +FG SPP         +    +ID  S +   +C RI+   +   + +S   G G S   
Sbjct: 27  LFGFSPPNSGKFINSSKLHCTKIDSRSIRSGIHCRRIVLDRNAFCDSDSISWGGGGSRVL 86

Query: 61  RLSRGSGGKNRH--LIANVASDFRNQSTSA-EAHVNQKSFETIYIQGGFTVKPLVIESIE 120
           R    S G+ R   +I +VASDFRN STS+ ++HVN KSFE+++      VKPLV + +E
Sbjct: 87  RARGSSRGRGRGVLVIPHVASDFRNYSTSSLDSHVNDKSFESMF------VKPLVFKEVE 146

Query: 121 TSRGLEKEEKSASEAEGLGSLKDFNYARV------ER--EASEIEKEAWSLLRDSVVNYC 180
            + G+ K E+       +G  KD N+  V      ER    +E+EKEAW LLR +VVNYC
Sbjct: 147 KTEGIPKRERG-----NVGGGKDANFGNVGVRKETERCLSQTEVEKEAWKLLRGAVVNYC 206

Query: 181 GHPVGTVAANDPADTQPLNYDQVFVRDFVPSALAFLLNGEDEIVKNFLLHTLQLQSWEKT 240
           G PVGTVAANDP DTQ LNYDQVF+RDFVPSA AF+L+GE EIV+NFLLHTLQLQSWEKT
Sbjct: 207 GFPVGTVAANDPGDTQTLNYDQVFIRDFVPSAYAFMLDGEGEIVRNFLLHTLQLQSWEKT 266

Query: 241 VDCYSPGQGLMPASFKVRSQPLDGSDGAFEEVLDPDFGESAIGRVAPVDSGLWWIILLRA 300
           VDC+SPG GLMPASFKV+S PL+G+DG+FEE LDPDFG SAIGRV+PVDSGLWWIILLRA
Sbjct: 267 VDCHSPGPGLMPASFKVKSAPLEGNDGSFEEFLDPDFGGSAIGRVSPVDSGLWWIILLRA 326

Query: 301 YGKITGDYTLQERVDVQTGIRLILNLCLTNGFDMFPTLLVSDGSCMIDRRMGIHGHPLEI 360
           YGK+TGDYTLQER+DVQTGI+LIL LCL +GFDMFPTLLV+DGSCM+DRRMGIHGHPLEI
Sbjct: 327 YGKLTGDYTLQERIDVQTGIKLILKLCLADGFDMFPTLLVTDGSCMVDRRMGIHGHPLEI 386

Query: 361 QALFYSALRCSREMLIVNDATKNLVAALNSRLSSLSFHIREYYWIDMNKINEIYRYKTEE 420
           QALFYSALRC+REMLIVND TK+LV A+N+RLS+LSFHIREYYW+D+ KINEIYRY TEE
Sbjct: 387 QALFYSALRCAREMLIVNDGTKSLVTAVNNRLSALSFHIREYYWVDIKKINEIYRYNTEE 446

Query: 421 YSSEAVNKFNIYPEQIPSWLVDWIPEEGGYLIGNLQPAHMDFRFFTLGNLWSIVSSLGTP 480
           YS++A NKFNIYPEQIP+WLVDWIP++GGY IGNLQPAHMDFRFFTLGNLW+++SSLG  
Sbjct: 447 YSADATNKFNIYPEQIPTWLVDWIPDKGGYFIGNLQPAHMDFRFFTLGNLWAVISSLGNQ 506

Query: 481 KQNKGILNLIEAKWDDLVANMPLKICFPAMEYEEWRIVTGSDPKNTPWSYHNGGSWPTLL 540
           +QN+G++ LIE KWDDLVANMPLKICFPA+E +EWRI+TGSDPKNTPWSYHNGGSWPTLL
Sbjct: 507 EQNEGVMTLIEEKWDDLVANMPLKICFPALEKDEWRIITGSDPKNTPWSYHNGGSWPTLL 566

Query: 541 WQFTLACMKMGRPELARNAIAVAEKKLSADRWPEYYDMRSARLIGKQSRLFQTWTIAGFL 600
           WQFTLAC+KMG+ ELA+ A+AVAEK+L  D WPEYYD +S R +GKQSRL+QTWTIAGFL
Sbjct: 567 WQFTLACIKMGKLELAKKAVAVAEKRLKEDEWPEYYDTKSGRFVGKQSRLYQTWTIAGFL 626

Query: 601 TSKLLLENPEKASLLYWEEDYELLQSCVCALGKANG--NRCSRQRHP 623
            +K L+E PEKASLL+WEEDY+LL++CVC L K++G  N+CSR   P
Sbjct: 627 AAKKLIEQPEKASLLFWEEDYQLLETCVCGLSKSSGRKNKCSRFTPP 662

BLAST of MS008229 vs. TAIR 10
Match: AT1G56560.1 (Plant neutral invertase family protein )

HSP 1 Score: 870.5 bits (2248), Expect = 8.0e-253
Identity = 423/599 (70.62%), Postives = 484/599 (80.80%), Query Frame = 0

Query: 22  PNCSRIIGSVQVNDNSSRRGFGFSNGYRLSRGSGGKNRHLIANVASDFRNQSTSAEAHVN 81
           P+ SR   +  +   SS + F  S+ Y   +     NR          R  STS E +++
Sbjct: 33  PDLSR---TTSIRHLSSSQRFVSSSIYCFPQSKILPNRFSEKTTGISVRQFSTSVETNLS 92

Query: 82  QKSFETIYIQGGFTVKPLVIESIETSRGLEKEEKSASEAE--GLGSLKDFNYARVEREAS 141
            KSFE I++Q              +   LE+  K+  E E   +GS K      V RE S
Sbjct: 93  DKSFERIHVQ--------------SDAILERIHKNEEEVETVSIGSEK------VVREES 152

Query: 142 EIEKEAWSLLRDSVVNYCGHPVGTVAANDPADTQPLNYDQVFVRDFVPSALAFLLNGEDE 201
           E EKEAW +L ++VV YCG PVGTVAANDP D  PLNYDQVF+RDFVPSALAFLL GE +
Sbjct: 153 EAEKEAWRILENAVVRYCGSPVGTVAANDPGDKMPLNYDQVFIRDFVPSALAFLLKGEGD 212

Query: 202 IVKNFLLHTLQLQSWEKTVDCYSPGQGLMPASFKVRSQPLDGSDGAFEEVLDPDFGESAI 261
           IV+NFLLHTLQLQSWEKTVDCYSPGQGLMPASFKVR+  LD  +   EEVLDPDFGESAI
Sbjct: 213 IVRNFLLHTLQLQSWEKTVDCYSPGQGLMPASFKVRTVALD--ENTTEEVLDPDFGESAI 272

Query: 262 GRVAPVDSGLWWIILLRAYGKITGDYTLQERVDVQTGIRLILNLCLTNGFDMFPTLLVSD 321
           GRVAPVDSGLWWIILLRAYGKITGD++LQER+DVQTGI+LI+NLCL +GFDMFPTLLV+D
Sbjct: 273 GRVAPVDSGLWWIILLRAYGKITGDFSLQERIDVQTGIKLIMNLCLADGFDMFPTLLVTD 332

Query: 322 GSCMIDRRMGIHGHPLEIQALFYSALRCSREMLIVNDATKNLVAALNSRLSSLSFHIREY 381
           GSCMIDRRMGIHGHPLEIQ+LFYSALRCSREML VND++K+LV A+N+RLS+LSFHIREY
Sbjct: 333 GSCMIDRRMGIHGHPLEIQSLFYSALRCSREMLSVNDSSKDLVRAINNRLSALSFHIREY 392

Query: 382 YWIDMNKINEIYRYKTEEYSSEAVNKFNIYPEQIPSWLVDWIPEEGGYLIGNLQPAHMDF 441
           YW+D+ KINEIYRYKTEEYS++A NKFNIYPEQIP WL+DWIPE+GGYL+GNLQPAHMDF
Sbjct: 393 YWVDIKKINEIYRYKTEEYSTDATNKFNIYPEQIPPWLMDWIPEQGGYLLGNLQPAHMDF 452

Query: 442 RFFTLGNLWSIVSSLGTPKQNKGILNLIEAKWDDLVANMPLKICFPAMEYEEWRIVTGSD 501
           RFFTLGN WSIVSSL TPKQN+ ILNLIEAKWDD++ NMPLKIC+PA+EY++WRI+TGSD
Sbjct: 453 RFFTLGNFWSIVSSLATPKQNEAILNLIEAKWDDIIGNMPLKICYPALEYDDWRIITGSD 512

Query: 502 PKNTPWSYHNGGSWPTLLWQFTLACMKMGRPELARNAIAVAEKKLSADRWPEYYDMRSAR 561
           PKNTPWSYHN GSWPTLLWQFTLACMKMGRPELA  A+AVAEK+L ADRWPEYYD RS +
Sbjct: 513 PKNTPWSYHNSGSWPTLLWQFTLACMKMGRPELAEKALAVAEKRLLADRWPEYYDTRSGK 572

Query: 562 LIGKQSRLFQTWTIAGFLTSKLLLENPEKASLLYWEEDYELLQSCVCALGKANGNRCSR 619
            IGKQSRL+QTWT+AGFLTSKLLL NPE ASLL+WEEDYELL  C C L K++  +CSR
Sbjct: 573 FIGKQSRLYQTWTVAGFLTSKLLLANPEMASLLFWEEDYELLDICACGLRKSDRKKCSR 606

BLAST of MS008229 vs. TAIR 10
Match: AT3G05820.1 (invertase H )

HSP 1 Score: 849.7 bits (2194), Expect = 1.5e-246
Identity = 401/558 (71.86%), Postives = 467/558 (83.69%), Query Frame = 0

Query: 63  ANVASDFRNQSTSAEAHVNQKSFETIYIQGGFTVKPLVIESIETSRGLEKEEKSASEAEG 122
           A V S+ R+ S S     N  + + IY + G  VKPLV+E ++     EK+E++ +E E 
Sbjct: 103 AQVVSEARSHSASTTC-ANDTTLDQIYTKNGLNVKPLVVERLKRD---EKDEEAVNEDE- 162

Query: 123 LGSLKDFNYARVEREASEIEKEAWSLLRDSVVNYCGHPVGTVAANDPADTQPLNYDQVFV 182
              +K   +  V  + +++E+EAW LLRDS+V YC  PVGTVAA DP DT P NYDQVF+
Sbjct: 163 -EGVKRDGFEGV--KCNDVEEEAWRLLRDSIVTYCDSPVGTVAAKDPTDTTPSNYDQVFI 222

Query: 183 RDFVPSALAFLLNGEDEIVKNFLLHTLQLQSWEKTVDCYSPGQGLMPASFKVRSQPLDGS 242
           RDFVPSALAFLL GE EIV+NFLLHTLQLQSWEKTVDCYSPGQGLMPASFKVR+ PL+  
Sbjct: 223 RDFVPSALAFLLKGESEIVRNFLLHTLQLQSWEKTVDCYSPGQGLMPASFKVRTLPLE-- 282

Query: 243 DGAFEEVLDPDFGESAIGRVAPVDSGLWWIILLRAYGKITGDYTLQERVDVQTGIRLILN 302
           +  FEEVLDPDFGE+AIGRVAPVDSGLWWIILLRAYGKITGDY+LQER+DVQTGI++I N
Sbjct: 283 EDKFEEVLDPDFGEAAIGRVAPVDSGLWWIILLRAYGKITGDYSLQERIDVQTGIKMIAN 342

Query: 303 LCLTNGFDMFPTLLVSDGSCMIDRRMGIHGHPLEIQALFYSALRCSREMLIVNDATKNLV 362
           LCL +GFDMFPTLLV+DGSCMIDRRMGIHGHPLEIQALFYSALR SREM+ VND++KN++
Sbjct: 343 LCLADGFDMFPTLLVTDGSCMIDRRMGIHGHPLEIQALFYSALRSSREMITVNDSSKNII 402

Query: 363 AALNSRLSSLSFHIREYYWIDMNKINEIYRYKTEEYSSEAVNKFNIYPEQIPSWLVDWIP 422
             +++RLS+LSFHIRE YW+D NKINEIYRYKTEEYS +A NKFNIYPEQ+  WL+DW+P
Sbjct: 403 KTISNRLSALSFHIRENYWVDKNKINEIYRYKTEEYSMDATNKFNIYPEQVSPWLMDWVP 462

Query: 423 E--EGGYLIGNLQPAHMDFRFFTLGNLWSIVSSLGTPKQNKGILNLIEAKWDDLVANMPL 482
           E  + G+LIGNLQPAHMDFRFFTLGNLWSI+SSLGTPKQN+ ILNL+E KWDDLV +MPL
Sbjct: 463 ESPDSGFLIGNLQPAHMDFRFFTLGNLWSIISSLGTPKQNQAILNLVEEKWDDLVGHMPL 522

Query: 483 KICFPAMEYEEWRIVTGSDPKNTPWSYHNGGSWPTLLWQFTLACMKMGRPELARNAIAVA 542
           KIC+PA+E  EW I+TGSDPKNTPWSYHNGGSWPTLLWQFTLAC+KMGRPELA  A+ +A
Sbjct: 523 KICYPALESSEWHIITGSDPKNTPWSYHNGGSWPTLLWQFTLACIKMGRPELAEKAVTLA 582

Query: 543 EKKLSADRWPEYYDMRSARLIGKQSRLFQTWTIAGFLTSKLLLENPEKASLLYWEEDYEL 602
           EK+L ADRWPEYYD R  + IGKQSRL+QTWTIAGFLTSK LL+NPE AS L+WEED EL
Sbjct: 583 EKRLQADRWPEYYDTRDGKFIGKQSRLYQTWTIAGFLTSKQLLQNPEIASSLFWEEDLEL 642

Query: 603 LQSCVCALGKANGNRCSR 619
           L+SCVC L K+   +CSR
Sbjct: 643 LESCVCVLTKSGRKKCSR 650

BLAST of MS008229 vs. TAIR 10
Match: AT5G22510.1 (alkaline/neutral invertase )

HSP 1 Score: 753.1 bits (1943), Expect = 1.9e-217
Identity = 368/571 (64.45%), Postives = 441/571 (77.23%), Query Frame = 0

Query: 58  NRHLIANVASDFRNQSTSAEAHVNQKSFETIYIQGGFTVKPLVIESIETSRGLEKEEKSA 117
           N H +++V     + S S      +K   TI       V+  +  ++  S GL  E KS 
Sbjct: 48  NSHEVSSVPKHSFHSSNSVLK--GKKFVSTICKCQKHDVEESIRSTLLPSDGLSSELKSD 107

Query: 118 SEAEGL---GSLKDFNYARVEREASEIEKEAWSLLRDSVVNYCGHPVGTVAANDPADTQP 177
            +   L   GS+     A+     S IE EAW LLR SVV YCG P+GT+AANDP  T  
Sbjct: 108 LDEMPLPVNGSVSSNGNAQSVGTKS-IEDEAWDLLRQSVVFYCGSPIGTIAANDPNSTSV 167

Query: 178 LNYDQVFVRDFVPSALAFLLNGEDEIVKNFLLHTLQLQSWEKTVDCYSPGQGLMPASFKV 237
           LNYDQVF+RDF+PS +AFLL GE +IV+NF+L+TLQLQSWEKT+DC+SPGQGLMP SFKV
Sbjct: 168 LNYDQVFIRDFIPSGIAFLLKGEYDIVRNFILYTLQLQSWEKTMDCHSPGQGLMPCSFKV 227

Query: 238 RSQPLDGSDGAFEEVLDPDFGESAIGRVAPVDSGLWWIILLRAYGKITGDYTLQERVDVQ 297
           ++ PLDG D   EEVLDPDFGE+AIGRVAPVDSGLWWIILLRAYGK TGD ++QERVDVQ
Sbjct: 228 KTVPLDGDDSMTEEVLDPDFGEAAIGRVAPVDSGLWWIILLRAYGKCTGDLSVQERVDVQ 287

Query: 298 TGIRLILNLCLTNGFDMFPTLLVSDGSCMIDRRMGIHGHPLEIQALFYSALRCSREMLIV 357
           TGI++IL LCL +GFDMFPTLLV+DGSCMIDRRMGIHGHPLEIQALFYSAL C+REML  
Sbjct: 288 TGIKMILKLCLADGFDMFPTLLVTDGSCMIDRRMGIHGHPLEIQALFYSALVCAREMLTP 347

Query: 358 NDATKNLVAALNSRLSSLSFHIREYYWIDMNKINEIYRYKTEEYSSEAVNKFNIYPEQIP 417
            D + +L+ ALN+RL +L+FHIREYYW+D+ KINEIYRY+TEEYS +AVNKFNIYP+QIP
Sbjct: 348 EDGSADLIRALNNRLVALNFHIREYYWLDLKKINEIYRYQTEEYSYDAVNKFNIYPDQIP 407

Query: 418 SWLVDWIPEEGGYLIGNLQPAHMDFRFFTLGNLWSIVSSLGTPKQNKGILNLIEAKWDDL 477
           SWLVD++P  GGYLIGNLQPAHMDFRFFTLGNLWSIVSSL +  Q+  IL+ IEAKW +L
Sbjct: 408 SWLVDFMPNRGGYLIGNLQPAHMDFRFFTLGNLWSIVSSLASNDQSHAILDFIEAKWAEL 467

Query: 478 VANMPLKICFPAMEYEEWRIVTGSDPKNTPWSYHNGGSWPTLLWQFTLACMKMGRPELAR 537
           VA+MPLKIC+PAME EEWRI+TGSDPKNTPWSYHNGG+WPTLLWQ T+A +KMGRPE+A 
Sbjct: 468 VADMPLKICYPAMEGEEWRIITGSDPKNTPWSYHNGGAWPTLLWQLTVASIKMGRPEIAE 527

Query: 538 NAIAVAEKKLSADRWPEYYDMRSARLIGKQSRLFQTWTIAGFLTSKLLLENPEKASLLYW 597
            A+ +AE+++S D+WPEYYD + AR IGKQ+RL+QTW+IAG+L +KLLL NP  A  L  
Sbjct: 528 KAVELAERRISLDKWPEYYDTKRARFIGKQARLYQTWSIAGYLVAKLLLANPAAAKFLTS 587

Query: 598 EEDYELLQSCVCALGKANGNRCSRQRHPARP 626
           EED +L  +  C L  AN  R    +   +P
Sbjct: 588 EEDSDLRNAFSCML-SANPRRTRGPKKAQQP 614

BLAST of MS008229 vs. TAIR 10
Match: AT4G34860.1 (Plant neutral invertase family protein )

HSP 1 Score: 592.4 bits (1526), Expect = 4.2e-169
Identity = 277/479 (57.83%), Postives = 358/479 (74.74%), Query Frame = 0

Query: 124 GSLKDFNYARVER--EASEIEKEAWSLLRDSVVNYCGHPVGTVAANDPADTQPLNYDQVF 183
           G    FN  R +   E+  +  EAW  LR S+V + G PVGT+AA D ++ + LNYDQVF
Sbjct: 87  GRRSGFNTPRSQPGFESHPMVGEAWDALRRSMVYFRGQPVGTIAAVDNSE-EKLNYDQVF 146

Query: 184 VRDFVPSALAFLLNGEDEIVKNFLLHTLQLQSWEKTVDCYSPGQGLMPASFKVRSQPLDG 243
           VRDFVPSALAFL+NGE +IVKNFLL TL+LQSWEK +D +  G+G+MPASFKV   P+  
Sbjct: 147 VRDFVPSALAFLMNGEPDIVKNFLLKTLRLQSWEKKIDRFQLGEGVMPASFKVFHDPVRN 206

Query: 244 SDGAFEEVLDPDFGESAIGRVAPVDSGLWWIILLRAYGKITGDYTLQERVDVQTGIRLIL 303
                 E L  DFGESAIGRVAPVDSG WWIILLRAY K TGD +L +  + Q GIRLIL
Sbjct: 207 -----HETLIADFGESAIGRVAPVDSGFWWIILLRAYTKSTGDSSLADMPECQKGIRLIL 266

Query: 304 NLCLTNGFDMFPTLLVSDGSCMIDRRMGIHGHPLEIQALFYSALRCSREMLIVNDATKNL 363
           +LCL+ GFD FPTLL +DG CMIDRRMG++G+P+EIQALF+ ALRC+  +L  +   K +
Sbjct: 267 SLCLSEGFDTFPTLLCADGCCMIDRRMGVYGYPIEIQALFFMALRCALLLLKHDGEGKEM 326

Query: 364 VAALNSRLSSLSFHIREYYWIDMNKINEIYRYKTEEYSSEAVNKFNIYPEQIPSWLVDWI 423
           V  +  RL +LS+H+R Y+W+D+ ++N+IYRYKTEEYS  AVNKFN+ P+ +P W+ D++
Sbjct: 327 VEQIVKRLHALSYHMRSYFWLDLKQLNDIYRYKTEEYSHTAVNKFNVIPDSLPEWVFDFM 386

Query: 424 PEEGGYLIGNLQPAHMDFRFFTLGNLWSIVSSLGTPKQNKGILNLIEAKWDDLVANMPLK 483
           P  GG+ IGN+ PA MDFR+F LGN  +I+SSL TP+Q+  I++LIE++W++LV  MPLK
Sbjct: 387 PPHGGFFIGNVSPARMDFRWFALGNCIAILSSLATPEQSTAIMDLIESRWEELVGEMPLK 446

Query: 484 ICFPAMEYEEWRIVTGSDPKNTPWSYHNGGSWPTLLWQFTLACMKMGRPELARNAIAVAE 543
           +C+PA+E  EWRIVTG DPKNT WSYHNGGSWP LLW  T AC+K GRP++AR AI VAE
Sbjct: 447 VCYPAIESHEWRIVTGCDPKNTRWSYHNGGSWPVLLWLLTAACIKTGRPQIARRAIEVAE 506

Query: 544 KKLSADRWPEYYDMRSARLIGKQSRLFQTWTIAGFLTSKLLLENPEKASLLYWEEDYEL 601
            +L  D WPEYYD +  R +GKQSR  QTW++AG+L +K++LE+P    ++  EED ++
Sbjct: 507 ARLHKDHWPEYYDGKVGRYVGKQSRKNQTWSVAGYLVAKMMLEDPSHVGMVCLEEDKQM 559

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022138141.10.0e+0099.84alkaline/neutral invertase A, mitochondrial [Momordica charantia][more]
XP_038875028.10.0e+0088.50alkaline/neutral invertase A, mitochondrial [Benincasa hispida][more]
XP_008438972.10.0e+0087.16PREDICTED: alkaline/neutral invertase A, mitochondrial [Cucumis melo][more]
XP_011651089.10.0e+0086.74alkaline/neutral invertase A, mitochondrial [Cucumis sativus] >KGN57182.1 hypoth... [more]
XP_022956128.10.0e+0085.94alkaline/neutral invertase A, mitochondrial-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
B9DFA83.0e-26067.85Alkaline/neutral invertase C, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=I... [more]
Q9FXA81.1e-25170.62Alkaline/neutral invertase A, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=I... [more]
Q84JL52.1e-24571.86Probable alkaline/neutral invertase A, chloroplastic OS=Arabidopsis thaliana OX=... [more]
Q10MC01.1e-24177.06Neutral/alkaline invertase 1, mitochondrial OS=Oryza sativa subsp. japonica OX=3... [more]
Q6H6N51.5e-21973.50Neutral/alkaline invertase 3, chloroplastic OS=Oryza sativa subsp. japonica OX=3... [more]
Match NameE-valueIdentityDescription
A0A6J1C8V30.0e+0099.84Alkaline/neutral invertase OS=Momordica charantia OX=3673 GN=LOC111009384 PE=3 S... [more]
A0A1S3AX880.0e+0087.16Alkaline/neutral invertase OS=Cucumis melo OX=3656 GN=LOC103483905 PE=3 SV=1[more]
A0A0A0L9680.0e+0086.74Alkaline/neutral invertase OS=Cucumis sativus OX=3659 GN=Csa_3G168930 PE=3 SV=1[more]
A0A6J1GVX40.0e+0085.94Alkaline/neutral invertase OS=Cucurbita moschata OX=3662 GN=LOC111457912 PE=3 SV... [more]
A0A6J1IXE10.0e+0085.47Alkaline/neutral invertase OS=Cucurbita maxima OX=3661 GN=LOC111479350 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT3G06500.12.1e-26167.85Plant neutral invertase family protein [more]
AT1G56560.18.0e-25370.62Plant neutral invertase family protein [more]
AT3G05820.11.5e-24671.86invertase H [more]
AT5G22510.11.9e-21764.45alkaline/neutral invertase [more]
AT4G34860.14.2e-16957.83Plant neutral invertase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 131..151
NoneNo IPR availablePANTHERPTHR31916:SF49ALKALINE/NEUTRAL INVERTASE C, MITOCHONDRIALcoord: 57..620
IPR024746Glycosyl hydrolase family 100PFAMPF12899Glyco_hydro_100coord: 145..586
e-value: 2.6E-215
score: 715.1
IPR024746Glycosyl hydrolase family 100PANTHERPTHR31916FAMILY NOT NAMEDcoord: 57..620
IPR012341Six-hairpin glycosidase-like superfamilyGENE3D1.50.10.10coord: 175..572
e-value: 5.8E-13
score: 50.5
IPR008928Six-hairpin glycosidase superfamilySUPERFAMILY48208Six-hairpin glycosidasescoord: 145..578

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS008229.1MS008229.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
molecular_function GO:0033926 glycopeptide alpha-N-acetylgalactosaminidase activity