MS008133 (gene) Bitter gourd (TR) v1

Overview
NameMS008133
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionAUGMIN subunit 4
Locationscaffold703: 488359 .. 492773 (+)
RNA-Seq ExpressionMS008133
SyntenyMS008133
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTGAAAAGCTTACAGGGCGGAGGGCAAAACCTTCCGGCGGACGTCACGCAGGTTATTGATCAGTTGGAGCGCCACTGCTTGGCTCCTGATGGATCCCTCGTCTCCAAGCCCTCCCACTACGATCTACAACTCGTATTCATTCTTCTCCTCTCGTTTTGTTCTCCCCTTCGATTTTTGTGTTTTATTGATTGATGAGGAAATGTGGGTCGAATCGAGCGTTGTAATTTGATCTCTGTTTGAAATCTATCAAGAAATTGACCTAATATTGCGTCGACTCATTGCTTCAGTTTATATATGTATATATGTACTGAATACTGAAGCTGAATTTTACTAATTATGAATTTTGCAGGCGAGGGAAGAAATGTCGAGGGAAAGATTGCGTTATTTGGAAGCTATGGTGGGTGTTCCACCCCTTTTTTTTTTTTATTATTTAGTTCTGGGTTGTGTGAGACACCACAATTGATTGAGTTGGTTGCATACTTGCATCCGTGGGCGATTATTTTTCTGTTTTCCCCGTGGGAGGAGTGTAATTGTTGTTGCGTGTTTCATGTAGGCAATCTATTGTGAAGCAATTGCAATGGTGGAAGAGTACCAACAGGCTGTTTCAATGGCGAACCTTGGAGGAGTTCGAGATGTTCAGGCTCTTTATCCTCAACTAGGCTTGAAGAATTCGCCTCAGGTTTGTGATAGGAACCGTTGTAAATTTACATTGTAAAAATGAAAATAAGAAGAATGATAGTTGATAGCCTTTTTGAAAGGGCACAATCAACTTCAAACTCTGCCTACCTCTCAAATCTCCTGACCTATATAACATTCAAACTCAGAGTGAACGAACTTATCTTCTAACAAAATCACCCAATCTCTAGAAAACTAATTAACCTATATAGATAACCTTGATTTCGAAAAGTTTTATTGTCTGCTCCGCTCCTGCAATTTGTTGAAAAATTACTTTGCAATATCACCTTTCTTATTTTAGTTGGAAGGATTATGTTCAACTTGTTTGTTTGGAAGTTCAAACTGAGTGGATCTCATGTACAAATGTTTGTTGGGATAATGTTATGATAGAATGAAGGATGGAAACATGTATTGAAAAGTGACGTTACTGGAAATACCATAGGTTTGCCCTCGGTTATACATGTTGTGAACTTCTAAGTGTATTTTTTTTTTTTTTTTTATTTTTATCATTGAAATTTTATCAGATTATATCTGAATGGGCCTTATTATTATGTTGCAGACGCTTTCAGTTTTTTTCCTATTAGAAAAAAAGGCCCTCAGTTGTTTTTGTGTGTTAGATTTGCTGAATATTGGTAGTCTGAACAACTTCCTGAAATTCAGGGCCTTAGAACTATGGTTCGGAATCAGATTTCTTTTTAGGATTGTATGTAACTAGTTATTCTAAGAAGCTTATAATTATTGGAAGTATTGCTTTTCAAAATTTTAATATTTTAACAGCCTCTATTATGCTATAGTCCTATCAACTTTCCATGCAGATGAAAAAAAGCCATCCTATCTTTTAGGGGATACTGAATAGATAAATTGGCTTTCAAGTATGTATCTAATTCTACGTCACACACAACTGCGTAGATGGAGTGATAGATTTCAATCCTTGTCTCAGGTTTACGAGACTCTTGAGCATCGAATGGTTGTGGCAGAAGCATCACAGAGATTGAGGCTTCCTCTTATATCAAAGGATGGTGAAATCCACGAGGAAGAGATCGAGAAGTTGAGTTTAATATCTCGAAGTTCCCTTGATAGTACAAGCACCAGTGTCACAATCAGCTCAAGCACTAACTCAACAAATTATGCAAGTGCAAATAGTACTGGAAGCATTGTGAACAATATTCTTTCGGTTAGCTCAACTGATACCGCCGAACCTGGAGTAGGTGGTGTTCCCAATCGCTTTCTTGGAATCACACCTGCCTTTTTATGGCAAACACAGCTCCATCAAACGCCTTCTATGGTATGACATCTATGTGGATGGGTGGAAAGTATCAAATTCTCAACGACGTATACATTTTCCCTTTTCTGTTGGCTTTTATTGAATTGGGTTATTTTTCAGACCGATTCTGTACCAGATATTATTTTTTAGCCACCTAATATTTTGCTCACAATTTATGAAAGGCATCACTGATCTAAATTATCTTCAACTTCTTCTAATGTCATTACAGGATATGACTGAATATCAAATGGCTCTTTCCCGTGAGATTGAGGCTCGTTTGAAGACTAAATGCGACAAGGTAGCTGATGCTTTTGTGATGGATGATATCGGTAAGTCTTTTGTTCCTTACCATTTATGTACAAACTTGCGAGCGTGTGTGTAATTGCGTTGAATTTTTTTTTCTCCCGGTTTTGTAGCTTTAGTTATATTTACTTGATTTTCATGTGTCGAAGTGTTGATATTCACTTGATTTGATGTGTCAAAAGTATTGATCTGTCTTTTTATCCTAGAACTGTTGGAAGGTTGCATCTTACATGTTTCAGTTCAACTACATTTATCCATGTGACGAGTGTTCACTTTTCTGCTACTTGATCTTGAAAAATGAAATTATATGGACTATGCTTTTAGTTTTAGTAGGTAATTCATTCTACAATGCATATCTTATTGCCGTACATTTATGCAGAGTCATCATCGGGGCATCATAGCTCTAGTGCAAGGCTTCCAGAGAGGTCTGGTTTACTCTTACGTGGCAATTGGCATTATTTCTTCTTCTTCTTTTTTTTTTTTTGTTTTTTTGTGCGTGTGTGTAAACCCCACATTTATATTTCTCGCTAAAACTTCAATAAATAGGGTGAAGTTGATAATCGAGGAAATTGAAAGGGAAGAAGCAGCTTTACGACAAGAGCTTTACTCAGCGGACAGAAAGTTTGCTGAATATTACAATGTATGACAGGAAGCTCATTTTAATATATGAGTACTTTTTATCCATTTTGGCAAAGTATAATTGCGTGAATTTTCTAATATTTTGTTCAAAACTAGGTTCTTGAGCAGATATTGGGAGTGCTGATTAAGCTTGTTAAAGATTTGAAGCTGCAGCACCAACATAAATATGTAATCACTGTTATTTTCTATCATAATTGATGGTTAAATTTTCTCGAATGACTTCTGTAATTCTATTTCCTTTGTAGTTTTCTTACTCTAGCACATTTAACCTGTTACCTGTTTGAATTGAAATTTTGTAGGACGACCTCCAAAAAACATGGCTGTGCAAAAGGTGCGAGACCATGAATGCAAAACTGAGGTTTGTCACAACTCAATGTTGATTGGATCCTCATGGTTATTAAATAGTTAGATATTCATACTCTTTTTTCACATATTTGACACATGTTGGGTGCGCATTGGACGTTGTCCGTGTTTGACGTCTATCTGACACTGGCACTCCAAAAAAAAAAATAGTGTCTGTGCTTCCTAGTTCATTTTACGATCAGGGCATCCTAACTCATGTTTCTGGCTAAATCCATCCATCTGAAAGTCCATAAAGAATTGCGTTTCATGCATCCCTCCTGTATCTCTGTTTTGGAGCTGTATACCATTTTAACTGCAAATTCTGTTCCATTTATCTAAATATGCATTATTCTGCGGCAATGCTGTACCTCATTTTTCAGTGTTTTAGAGCACGTTCTCCTGCTAGAGACCTACACTCAGGAATCTATACCAGCCCTTCATAAAATAAGGTACGTGTATTTTGTTGATTGTATTCTTTTCAGCAGCCAGCGTGTTATCTTTTGGAGGGTTAATATGATTAGAATGTGTTTCTTTGCAGAAAGTATCTTGTTGAAGCTACGGAGGAAGCTTCTATTTCTTATAATAAAGCAGTAAGTCTCTCTCTCGTGTTCTCGCTCTCTATATATATTGATAAAAGAAGGAGAATATATTCACAAATGTAATAAACAATTTCTCTCGATCTTGGAGGCTTCTGTGATTTCTTTCTAACCAAAAAAGATTAAAAAAACACGCTAACCGAAGCACTTCAGCTTTGTCTTTCCACCAGCGGTCCAGCACCATAGTGGAGGGGGAGGAAGAACAAATTTATGTTTATATATGACCTTTCTATGTTGTTTGATCATTAAACCTTATAGTATTTGACCTGATCTGTAATCTTTGCAACTTCAGTTTGAATTCACTGGTTCATGCTACATCGTTTACGTTTACGTTTTTGCCAGGTTACTCGTCTCCGCGAGTATCAAGGTGTAGACCCCCACTTCGACACAATCGCAAGGCAGTACCACGATATTGTAATGGTAATGTGTCGTGAAATAGTTTCTCACTTTCATCCTCTCTGTGAAAAATGGGGAAGAAAATAAAGGCTCATCAACTTTCACCCACTTTTGTTTTGCAGAAACTGGAAAATATGCAGTGGACAATTCACCAAGTTGAAATGGACTTGAAACGCTTACCAGATCAATCAAGTACA

mRNA sequence

ATGGTGAAAAGCTTACAGGGCGGAGGGCAAAACCTTCCGGCGGACGTCACGCAGGTTATTGATCAGTTGGAGCGCCACTGCTTGGCTCCTGATGGATCCCTCGTCTCCAAGCCCTCCCACTACGATCTACAACTCGCGAGGGAAGAAATGTCGAGGGAAAGATTGCGTTATTTGGAAGCTATGGCAATCTATTGTGAAGCAATTGCAATGGTGGAAGAGTACCAACAGGCTGTTTCAATGGCGAACCTTGGAGGAGTTCGAGATGTTCAGGCTCTTTATCCTCAACTAGGCTTGAAGAATTCGCCTCAGGTTTACGAGACTCTTGAGCATCGAATGGTTGTGGCAGAAGCATCACAGAGATTGAGGCTTCCTCTTATATCAAAGGATGGTGAAATCCACGAGGAAGAGATCGAGAAGTTGAGTTTAATATCTCGAAGTTCCCTTGATAGTACAAGCACCAGTGTCACAATCAGCTCAAGCACTAACTCAACAAATTATGCAAGTGCAAATAGTACTGGAAGCATTGTGAACAATATTCTTTCGGTTAGCTCAACTGATACCGCCGAACCTGGAGTAGGTGGTGTTCCCAATCGCTTTCTTGGAATCACACCTGCCTTTTTATGGCAAACACAGCTCCATCAAACGCCTTCTATGGATATGACTGAATATCAAATGGCTCTTTCCCGTGAGATTGAGGCTCGTTTGAAGACTAAATGCGACAAGGTAGCTGATGCTTTTGTGATGGATGATATCGAGTCATCATCGGGGCATCATAGCTCTAGTGCAAGGCTTCCAGAGAGGGTGAAGTTGATAATCGAGGAAATTGAAAGGGAAGAAGCAGCTTTACGACAAGAGCTTTACTCAGCGGACAGAAAGTTTGCTGAATATTACAATGTTCTTGAGCAGATATTGGGAGTGCTGATTAAGCTTGTTAAAGATTTGAAGCTGCAGCACCAACATAAATATGACGACCTCCAAAAAACATGGCTGTGCAAAAGGTGCGAGACCATGAATGCAAAACTGAGTGTTTTAGAGCACGTTCTCCTGCTAGAGACCTACACTCAGGAATCTATACCAGCCCTTCATAAAATAAGAAAGTATCTTGTTGAAGCTACGGAGGAAGCTTCTATTTCTTATAATAAAGCAGTTACTCGTCTCCGCGAGTATCAAGGTGTAGACCCCCACTTCGACACAATCGCAAGGCAGTACCACGATATTGTAATGAAACTGGAAAATATGCAGTGGACAATTCACCAAGTTGAAATGGACTTGAAACGCTTACCAGATCAATCAAGTACA

Coding sequence (CDS)

ATGGTGAAAAGCTTACAGGGCGGAGGGCAAAACCTTCCGGCGGACGTCACGCAGGTTATTGATCAGTTGGAGCGCCACTGCTTGGCTCCTGATGGATCCCTCGTCTCCAAGCCCTCCCACTACGATCTACAACTCGCGAGGGAAGAAATGTCGAGGGAAAGATTGCGTTATTTGGAAGCTATGGCAATCTATTGTGAAGCAATTGCAATGGTGGAAGAGTACCAACAGGCTGTTTCAATGGCGAACCTTGGAGGAGTTCGAGATGTTCAGGCTCTTTATCCTCAACTAGGCTTGAAGAATTCGCCTCAGGTTTACGAGACTCTTGAGCATCGAATGGTTGTGGCAGAAGCATCACAGAGATTGAGGCTTCCTCTTATATCAAAGGATGGTGAAATCCACGAGGAAGAGATCGAGAAGTTGAGTTTAATATCTCGAAGTTCCCTTGATAGTACAAGCACCAGTGTCACAATCAGCTCAAGCACTAACTCAACAAATTATGCAAGTGCAAATAGTACTGGAAGCATTGTGAACAATATTCTTTCGGTTAGCTCAACTGATACCGCCGAACCTGGAGTAGGTGGTGTTCCCAATCGCTTTCTTGGAATCACACCTGCCTTTTTATGGCAAACACAGCTCCATCAAACGCCTTCTATGGATATGACTGAATATCAAATGGCTCTTTCCCGTGAGATTGAGGCTCGTTTGAAGACTAAATGCGACAAGGTAGCTGATGCTTTTGTGATGGATGATATCGAGTCATCATCGGGGCATCATAGCTCTAGTGCAAGGCTTCCAGAGAGGGTGAAGTTGATAATCGAGGAAATTGAAAGGGAAGAAGCAGCTTTACGACAAGAGCTTTACTCAGCGGACAGAAAGTTTGCTGAATATTACAATGTTCTTGAGCAGATATTGGGAGTGCTGATTAAGCTTGTTAAAGATTTGAAGCTGCAGCACCAACATAAATATGACGACCTCCAAAAAACATGGCTGTGCAAAAGGTGCGAGACCATGAATGCAAAACTGAGTGTTTTAGAGCACGTTCTCCTGCTAGAGACCTACACTCAGGAATCTATACCAGCCCTTCATAAAATAAGAAAGTATCTTGTTGAAGCTACGGAGGAAGCTTCTATTTCTTATAATAAAGCAGTTACTCGTCTCCGCGAGTATCAAGGTGTAGACCCCCACTTCGACACAATCGCAAGGCAGTACCACGATATTGTAATGAAACTGGAAAATATGCAGTGGACAATTCACCAAGTTGAAATGGACTTGAAACGCTTACCAGATCAATCAAGTACA

Protein sequence

MVKSLQGGGQNLPADVTQVIDQLERHCLAPDGSLVSKPSHYDLQLAREEMSRERLRYLEAMAIYCEAIAMVEEYQQAVSMANLGGVRDVQALYPQLGLKNSPQVYETLEHRMVVAEASQRLRLPLISKDGEIHEEEIEKLSLISRSSLDSTSTSVTISSSTNSTNYASANSTGSIVNNILSVSSTDTAEPGVGGVPNRFLGITPAFLWQTQLHQTPSMDMTEYQMALSREIEARLKTKCDKVADAFVMDDIESSSGHHSSSARLPERVKLIIEEIEREEAALRQELYSADRKFAEYYNVLEQILGVLIKLVKDLKLQHQHKYDDLQKTWLCKRCETMNAKLSVLEHVLLLETYTQESIPALHKIRKYLVEATEEASISYNKAVTRLREYQGVDPHFDTIARQYHDIVMKLENMQWTIHQVEMDLKRLPDQSST
Homology
BLAST of MS008133 vs. NCBI nr
Match: XP_022139359.1 (AUGMIN subunit 4 [Momordica charantia])

HSP 1 Score: 822.8 bits (2124), Expect = 1.4e-234
Identity = 429/433 (99.08%), Postives = 431/433 (99.54%), Query Frame = 0

Query: 1   MVKSLQGGGQNLPADVTQVIDQLERHCLAPDGSLVSKPSHYDLQLAREEMSRERLRYLEA 60
           MVKSLQGGGQNLPADVTQVIDQLERHCLAPDGSLVSKPSHYDLQLAREEMSRERLRYLEA
Sbjct: 1   MVKSLQGGGQNLPADVTQVIDQLERHCLAPDGSLVSKPSHYDLQLAREEMSRERLRYLEA 60

Query: 61  MAIYCEAIAMVEEYQQAVSMANLGGVRDVQALYPQLGLKNSPQVYETLEHRMVVAEASQR 120
           MA+YCEAIAMVEEYQQAVSMANLGG RDVQALYPQLGLKNSPQVYETLEHRMVVAEASQR
Sbjct: 61  MAVYCEAIAMVEEYQQAVSMANLGGFRDVQALYPQLGLKNSPQVYETLEHRMVVAEASQR 120

Query: 121 LRLPLISKDGEIHEEEIEKLSLISRSSLDSTSTSVTISSSTNSTNYASANSTGSIVNNIL 180
           LRLP+ISKDGEIHEEEIEKLSLISRSSLDSTSTSVTISSSTNSTNYASANSTGSIVNNIL
Sbjct: 121 LRLPJISKDGEIHEEEIEKLSLISRSSLDSTSTSVTISSSTNSTNYASANSTGSIVNNIL 180

Query: 181 SVSSTDTAEPGVGGVPNRFLGITPAFLWQTQLHQTPSMDMTEYQMALSREIEARLKTKCD 240
           SVSSTDTAEPGVGGVPN FLGITPAFLWQTQLHQTPSMDMTEYQMALSREIEARLKTKCD
Sbjct: 181 SVSSTDTAEPGVGGVPNSFLGITPAFLWQTQLHQTPSMDMTEYQMALSREIEARLKTKCD 240

Query: 241 KVADAFVMDDIESSSGHHSSSARLPERVKLIIEEIEREEAALRQELYSADRKFAEYYNVL 300
           KVADAFVMDDIESSSGHHSSSARLPERVKLIIEEIEREEAALRQELYSADRKFAEYYNVL
Sbjct: 241 KVADAFVMDDIESSSGHHSSSARLPERVKLIIEEIEREEAALRQELYSADRKFAEYYNVL 300

Query: 301 EQILGVLIKLVKDLKLQHQHKYDDLQKTWLCKRCETMNAKLSVLEHVLLLETYTQESIPA 360
           EQILGVLIKLVKDLKLQHQHKYDDLQKTWLCKRCETMNAKLSVLEHVLLLETYTQESIPA
Sbjct: 301 EQILGVLIKLVKDLKLQHQHKYDDLQKTWLCKRCETMNAKLSVLEHVLLLETYTQESIPA 360

Query: 361 LHKIRKYLVEATEEASISYNKAVTRLREYQGVDPHFDTIARQYHDIVMKLENMQWTIHQV 420
           LHKIRKYLVEATEEASISYNKAVTRLREYQGVDPHFDTIARQYHDIVMKLENMQWTIHQV
Sbjct: 361 LHKIRKYLVEATEEASISYNKAVTRLREYQGVDPHFDTIARQYHDIVMKLENMQWTIHQV 420

Query: 421 EMDLKRLPDQSST 434
           EMDLKRLPDQSST
Sbjct: 421 EMDLKRLPDQSST 433

BLAST of MS008133 vs. NCBI nr
Match: XP_008447737.1 (PREDICTED: AUGMIN subunit 4 [Cucumis melo])

HSP 1 Score: 803.9 bits (2075), Expect = 6.8e-229
Identity = 421/433 (97.23%), Postives = 426/433 (98.38%), Query Frame = 0

Query: 1   MVKSLQGGGQNLPADVTQVIDQLERHCLAPDGSLVSKPSHYDLQLAREEMSRERLRYLEA 60
           MVKSLQGGGQNLPADVTQVIDQLERHCLAPDGSLVSKP+HYDLQLAREEMSRERLRYLEA
Sbjct: 1   MVKSLQGGGQNLPADVTQVIDQLERHCLAPDGSLVSKPAHYDLQLAREEMSRERLRYLEA 60

Query: 61  MAIYCEAIAMVEEYQQAVSMANLGGVRDVQALYPQLGLKNSPQVYETLEHRMVVAEASQR 120
           MAIYCEAIAMVEEYQQAVSMANLGGVRDVQALYPQLGLKNSPQVYETLEHRMVVAEASQR
Sbjct: 61  MAIYCEAIAMVEEYQQAVSMANLGGVRDVQALYPQLGLKNSPQVYETLEHRMVVAEASQR 120

Query: 121 LRLPLISKDGEIHEEEIEKLSLISRSSLDSTSTSVTISSSTNSTNYASANSTGSIVNNIL 180
           LRLPLISKDGEIHEEEIEKL   SRSSLDSTSTSVTISSSTNSTNYASA+STGSIVNN L
Sbjct: 121 LRLPLISKDGEIHEEEIEKL---SRSSLDSTSTSVTISSSTNSTNYASASSTGSIVNNSL 180

Query: 181 SVSSTDTAEPGVGGVPNRFLGITPAFLWQTQLHQTPSMDMTEYQMALSREIEARLKTKCD 240
           SVSSTDTAEPGVGGVPNRFLGITPAFLWQTQLHQTPS DM EYQMALSREI++RLKTKCD
Sbjct: 181 SVSSTDTAEPGVGGVPNRFLGITPAFLWQTQLHQTPSTDMAEYQMALSREIDSRLKTKCD 240

Query: 241 KVADAFVMDDIESSSGHHSSSARLPERVKLIIEEIEREEAALRQELYSADRKFAEYYNVL 300
           KVADAF+MDDIESSSGHHSSSARLPERVKLIIEEIEREEAALRQELYSADRKFAEYYNVL
Sbjct: 241 KVADAFIMDDIESSSGHHSSSARLPERVKLIIEEIEREEAALRQELYSADRKFAEYYNVL 300

Query: 301 EQILGVLIKLVKDLKLQHQHKYDDLQKTWLCKRCETMNAKLSVLEHVLLLETYTQESIPA 360
           EQILGVLIKLVKDLKLQHQHKYDDLQKTWLCKRCETMNAKLSVLEHVLLLETYTQESIPA
Sbjct: 301 EQILGVLIKLVKDLKLQHQHKYDDLQKTWLCKRCETMNAKLSVLEHVLLLETYTQESIPA 360

Query: 361 LHKIRKYLVEATEEASISYNKAVTRLREYQGVDPHFDTIARQYHDIVMKLENMQWTIHQV 420
           LHKIRKYLVEATEEASISYNKAVTRLREYQGVDPHFD IARQYHDIVMKLENMQWTIHQV
Sbjct: 361 LHKIRKYLVEATEEASISYNKAVTRLREYQGVDPHFDNIARQYHDIVMKLENMQWTIHQV 420

Query: 421 EMDLKRLPDQSST 434
           EMDLKRLPDQSST
Sbjct: 421 EMDLKRLPDQSST 430

BLAST of MS008133 vs. NCBI nr
Match: KAA0025153.1 (AUGMIN subunit 4 [Cucumis melo var. makuwa] >TYK23111.1 AUGMIN subunit 4 [Cucumis melo var. makuwa])

HSP 1 Score: 798.9 bits (2062), Expect = 2.2e-227
Identity = 419/433 (96.77%), Postives = 424/433 (97.92%), Query Frame = 0

Query: 1   MVKSLQGGGQNLPADVTQVIDQLERHCLAPDGSLVSKPSHYDLQLAREEMSRERLRYLEA 60
           MVKSLQGGGQNLPADVTQVIDQLERHCLAPDGSLVSKP+HYDLQLAREEMSRERLRYLEA
Sbjct: 1   MVKSLQGGGQNLPADVTQVIDQLERHCLAPDGSLVSKPAHYDLQLAREEMSRERLRYLEA 60

Query: 61  MAIYCEAIAMVEEYQQAVSMANLGGVRDVQALYPQLGLKNSPQVYETLEHRMVVAEASQR 120
           MAIYCEAIAMVEEYQQAVSMANLGGVRDVQALYPQLGLKNSPQVYETLEHRMVVAEASQ 
Sbjct: 61  MAIYCEAIAMVEEYQQAVSMANLGGVRDVQALYPQLGLKNSPQVYETLEHRMVVAEASQG 120

Query: 121 LRLPLISKDGEIHEEEIEKLSLISRSSLDSTSTSVTISSSTNSTNYASANSTGSIVNNIL 180
           LRLPLISKDGEIHEEEIEKL   SRSSLDSTSTSVTI SSTNSTNYASA+STGSIVNN L
Sbjct: 121 LRLPLISKDGEIHEEEIEKL---SRSSLDSTSTSVTIISSTNSTNYASASSTGSIVNNSL 180

Query: 181 SVSSTDTAEPGVGGVPNRFLGITPAFLWQTQLHQTPSMDMTEYQMALSREIEARLKTKCD 240
           SVSSTDTAEPGVGGVPNRFLGITPAFLWQTQLHQTPS DM EYQMALSREI++RLKTKCD
Sbjct: 181 SVSSTDTAEPGVGGVPNRFLGITPAFLWQTQLHQTPSTDMAEYQMALSREIDSRLKTKCD 240

Query: 241 KVADAFVMDDIESSSGHHSSSARLPERVKLIIEEIEREEAALRQELYSADRKFAEYYNVL 300
           KVADAF+MDDIESSSGHHSSSARLPERVKLIIEEIEREEAALRQELYSADRKFAEYYNVL
Sbjct: 241 KVADAFIMDDIESSSGHHSSSARLPERVKLIIEEIEREEAALRQELYSADRKFAEYYNVL 300

Query: 301 EQILGVLIKLVKDLKLQHQHKYDDLQKTWLCKRCETMNAKLSVLEHVLLLETYTQESIPA 360
           EQILGVLIKLVKDLKLQHQHKYDDLQKTWLCKRCETMNAKLSVLEHVLLLETYTQESIPA
Sbjct: 301 EQILGVLIKLVKDLKLQHQHKYDDLQKTWLCKRCETMNAKLSVLEHVLLLETYTQESIPA 360

Query: 361 LHKIRKYLVEATEEASISYNKAVTRLREYQGVDPHFDTIARQYHDIVMKLENMQWTIHQV 420
           LHKIRKYLVEATEEASISYNKAVTRLREYQGVDPHFD IARQYHDIVMKLENMQWTIHQV
Sbjct: 361 LHKIRKYLVEATEEASISYNKAVTRLREYQGVDPHFDNIARQYHDIVMKLENMQWTIHQV 420

Query: 421 EMDLKRLPDQSST 434
           EMDLKRLPDQSST
Sbjct: 421 EMDLKRLPDQSST 430

BLAST of MS008133 vs. NCBI nr
Match: XP_031744847.1 (AUGMIN subunit 4 [Cucumis sativus] >KGN44473.2 hypothetical protein Csa_016636 [Cucumis sativus])

HSP 1 Score: 797.7 bits (2059), Expect = 4.9e-227
Identity = 417/432 (96.53%), Postives = 423/432 (97.92%), Query Frame = 0

Query: 1   MVKSLQGGGQNLPADVTQVIDQLERHCLAPDGSLVSKPSHYDLQLAREEMSRERLRYLEA 60
           MVKSLQGGGQNLP DVTQVIDQLERHCLAPDGSLVSKP+HYDLQLAREEMSRERLRYLEA
Sbjct: 1   MVKSLQGGGQNLPTDVTQVIDQLERHCLAPDGSLVSKPAHYDLQLAREEMSRERLRYLEA 60

Query: 61  MAIYCEAIAMVEEYQQAVSMANLGGVRDVQALYPQLGLKNSPQVYETLEHRMVVAEASQR 120
           MAIYCEAIAMVEEYQQAVSMANLGGVRDVQALYPQLGLKNSPQVYETLEHRMVVAEASQR
Sbjct: 61  MAIYCEAIAMVEEYQQAVSMANLGGVRDVQALYPQLGLKNSPQVYETLEHRMVVAEASQR 120

Query: 121 LRLPLISKDGEIHEEEIEKLSLISRSSLDSTSTSVTISSSTNSTNYASANSTGSIVNNIL 180
           LRLPLISKDGEIHEEEIEKL   SRSSLDSTST VTISSSTNSTNYASA+STGSIVNN L
Sbjct: 121 LRLPLISKDGEIHEEEIEKL---SRSSLDSTSTGVTISSSTNSTNYASASSTGSIVNNSL 180

Query: 181 SVSSTDTAEPGVGGVPNRFLGITPAFLWQTQLHQTPSMDMTEYQMALSREIEARLKTKCD 240
           SVSSTDTAEPGVGGVPNRFLGITPAFLWQTQLH TPS DM EYQMALSREI+ARLKTKCD
Sbjct: 181 SVSSTDTAEPGVGGVPNRFLGITPAFLWQTQLHHTPSTDMAEYQMALSREIDARLKTKCD 240

Query: 241 KVADAFVMDDIESSSGHHSSSARLPERVKLIIEEIEREEAALRQELYSADRKFAEYYNVL 300
           KVADAF+MDDIESSSGHHSSSARLPERVKLIIEEIEREEAALRQELYSADRKFAEYYNVL
Sbjct: 241 KVADAFIMDDIESSSGHHSSSARLPERVKLIIEEIEREEAALRQELYSADRKFAEYYNVL 300

Query: 301 EQILGVLIKLVKDLKLQHQHKYDDLQKTWLCKRCETMNAKLSVLEHVLLLETYTQESIPA 360
           EQILGVLIKLVKDLKLQHQHKYDDLQKTWLCKRCETMNAKLSVLEHVLLLETYTQESIPA
Sbjct: 301 EQILGVLIKLVKDLKLQHQHKYDDLQKTWLCKRCETMNAKLSVLEHVLLLETYTQESIPA 360

Query: 361 LHKIRKYLVEATEEASISYNKAVTRLREYQGVDPHFDTIARQYHDIVMKLENMQWTIHQV 420
           LHKIRKYLVEATEEASISYNKAVTRLREYQGVDPHFDTIARQYHDIVMKL+NMQWTIHQV
Sbjct: 361 LHKIRKYLVEATEEASISYNKAVTRLREYQGVDPHFDTIARQYHDIVMKLDNMQWTIHQV 420

Query: 421 EMDLKRLPDQSS 433
           EMDLKRLPDQS+
Sbjct: 421 EMDLKRLPDQST 429

BLAST of MS008133 vs. NCBI nr
Match: XP_038900104.1 (AUGMIN subunit 4 isoform X1 [Benincasa hispida])

HSP 1 Score: 796.2 bits (2055), Expect = 1.4e-226
Identity = 416/433 (96.07%), Postives = 425/433 (98.15%), Query Frame = 0

Query: 1   MVKSLQGGGQNLPADVTQVIDQLERHCLAPDGSLVSKPSHYDLQLAREEMSRERLRYLEA 60
           MVKSLQGGGQNLPADVTQVIDQLERHCLAPDGSLVSKP HYDLQLAREEMSRERLRYLEA
Sbjct: 1   MVKSLQGGGQNLPADVTQVIDQLERHCLAPDGSLVSKPVHYDLQLAREEMSRERLRYLEA 60

Query: 61  MAIYCEAIAMVEEYQQAVSMANLGGVRDVQALYPQLGLKNSPQVYETLEHRMVVAEASQR 120
           MAIYCEAIAMVEEYQQAVSMANLGGVRDVQALYPQLGLKNSPQVYETLEHRMVVAEASQR
Sbjct: 61  MAIYCEAIAMVEEYQQAVSMANLGGVRDVQALYPQLGLKNSPQVYETLEHRMVVAEASQR 120

Query: 121 LRLPLISKDGEIHEEEIEKLSLISRSSLDSTSTSVTISSSTNSTNYASANSTGSIVNNIL 180
           LRLPLISKDGEI E+EIEK S++SRSSLDSTST +TISS+ NSTNYASA+STGSIVNN  
Sbjct: 121 LRLPLISKDGEIQEDEIEKWSVVSRSSLDSTSTGITISSTANSTNYASASSTGSIVNN-S 180

Query: 181 SVSSTDTAEPGVGGVPNRFLGITPAFLWQTQLHQTPSMDMTEYQMALSREIEARLKTKCD 240
           S++STDTAEPGVGGVPNRFLGITPAFLWQTQLHQTPSMDMTEYQ ALSREI+ARLKTKCD
Sbjct: 181 SLNSTDTAEPGVGGVPNRFLGITPAFLWQTQLHQTPSMDMTEYQKALSREIDARLKTKCD 240

Query: 241 KVADAFVMDDIESSSGHHSSSARLPERVKLIIEEIEREEAALRQELYSADRKFAEYYNVL 300
           KVADAFVMDDIESSSGHHSSSARLPERVKLIIEEIEREEAALRQELYSADRKFAEYYNVL
Sbjct: 241 KVADAFVMDDIESSSGHHSSSARLPERVKLIIEEIEREEAALRQELYSADRKFAEYYNVL 300

Query: 301 EQILGVLIKLVKDLKLQHQHKYDDLQKTWLCKRCETMNAKLSVLEHVLLLETYTQESIPA 360
           EQILGVLIKLVKDLKLQHQHKYDDLQKTWLCKRCETMNAKLSVLEHVLLLETYTQESIPA
Sbjct: 301 EQILGVLIKLVKDLKLQHQHKYDDLQKTWLCKRCETMNAKLSVLEHVLLLETYTQESIPA 360

Query: 361 LHKIRKYLVEATEEASISYNKAVTRLREYQGVDPHFDTIARQYHDIVMKLENMQWTIHQV 420
           LHKIRKYLVEATEEASISYNKAVTRLREYQGVDPHFDTIARQYHDIVMKLENMQWTIHQV
Sbjct: 361 LHKIRKYLVEATEEASISYNKAVTRLREYQGVDPHFDTIARQYHDIVMKLENMQWTIHQV 420

Query: 421 EMDLKRLPDQSST 434
           EMDLKRLPDQSST
Sbjct: 421 EMDLKRLPDQSST 432

BLAST of MS008133 vs. ExPASy Swiss-Prot
Match: Q8GYM3 (AUGMIN subunit 4 OS=Arabidopsis thaliana OX=3702 GN=AUG4 PE=1 SV=1)

HSP 1 Score: 637.9 bits (1644), Expect = 8.4e-182
Identity = 338/425 (79.53%), Postives = 378/425 (88.94%), Query Frame = 0

Query: 1   MVKSLQGGGQNLPADVTQVIDQLERHCLAPDGSLVSKPSHYDLQLAREEMSRERLRYLEA 60
           MVK+LQG  QNLPADV Q+IDQLERHCLAPDGSLV+K  + DLQLAREEMSRERLRYLEA
Sbjct: 1   MVKALQGAAQNLPADVNQLIDQLERHCLAPDGSLVTKSVYSDLQLAREEMSRERLRYLEA 60

Query: 61  MAIYCEAIAMVEEYQQAVSMANLGGVRDVQALYPQLGLKNSPQVYETLEHRMVVAEASQR 120
           MAIYCEA+AMVEEYQQA+S+AN GG+RDVQ LYPQLGLKNSPQVYETLEHR+VVAEA+Q+
Sbjct: 61  MAIYCEAVAMVEEYQQAISVANHGGIRDVQGLYPQLGLKNSPQVYETLEHRLVVAEAAQK 120

Query: 121 LRLPLISKDGEIHEEEIEKLSLISRSSLDSTSTSVTISSSTNSTNYASANSTGSIVNNIL 180
           LRLPLIS  GEIHEEEIEK S++SRSSLDS STS TISS++NS NY  ANS+ + V   +
Sbjct: 121 LRLPLISDGGEIHEEEIEKWSILSRSSLDSASTSFTISSTSNSVNY--ANSSANSVAGGI 180

Query: 181 SVSSTDTAEPGVGGVPNRFLGITPAFLWQTQLHQTPSMDMTEYQMALSREIEARLKTKCD 240
           S+S+ DT    VGGVPNRFLGITPA+L   QL  T SMDM +YQM L+REIE RLK KCD
Sbjct: 181 SLSAVDT--DVVGGVPNRFLGITPAYLSYVQLQNTISMDMADYQMFLAREIEGRLKEKCD 240

Query: 241 KVADAFVMDDIESSSGHHSSSARLPERVKLIIEEIEREEAALRQELYSADRKFAEYYNVL 300
           K+ADA ++DD +SS+G+ +SSARLPERVK IIEEIER+EAALR++LYSADRKFAEYYNVL
Sbjct: 241 KLADA-IVDDTDSSTGNRNSSARLPERVKFIIEEIERDEAALREDLYSADRKFAEYYNVL 300

Query: 301 EQILGVLIKLVKDLKLQHQHKYDDLQKTWLCKRCETMNAKLSVLEHVLLLETYTQESIPA 360
           EQILGVLIKLVKDLKL+HQHKY+++QKTWLCKRCETMNAKL VLE+VLLLETYT +SI A
Sbjct: 301 EQILGVLIKLVKDLKLEHQHKYNEMQKTWLCKRCETMNAKLRVLENVLLLETYTPDSISA 360

Query: 361 LHKIRKYLVEATEEASISYNKAVTRLREYQGVDPHFDTIARQYHDIVMKLENMQWTIHQV 420
           LH IR YLVEATEEAS +YNKAVTRLREYQGVDPHFDTIARQYHDIV KLENMQWTIHQV
Sbjct: 361 LHNIRNYLVEATEEASAAYNKAVTRLREYQGVDPHFDTIARQYHDIVKKLENMQWTIHQV 420

Query: 421 EMDLK 426
           EMDLK
Sbjct: 421 EMDLK 420

BLAST of MS008133 vs. ExPASy Swiss-Prot
Match: Q9H6D7 (HAUS augmin-like complex subunit 4 OS=Homo sapiens OX=9606 GN=HAUS4 PE=1 SV=1)

HSP 1 Score: 58.2 bits (139), Expect = 2.8e-07
Identity = 47/204 (23.04%), Postives = 95/204 (46.57%), Query Frame = 0

Query: 217 SMDMTEYQMALSREIEARLKTKCDKVADAF-VMDDIESSSGHHSSSARLPERVKLIIEEI 276
           S D    +  L +E+E +LK KC  +   +    D +S +   +   +L E +    ++ 
Sbjct: 154 SEDFVWMRARLQQEVEEQLKKKCFTLLCYYDPNSDADSETVKAAKVWKLAEVLVGEQQQC 213

Query: 277 EREEAALRQELYSADRKFAEYYNVLEQILGVLIKLVKDLKLQHQHKYDDLQKTWLCKRCE 336
           +  ++  ++++   ++K A Y  VL + L +L +L+++ +L+ Q + D +   +L  +C 
Sbjct: 214 QDAKSQQKEQMLLLEKKSAAYSQVLLRCLTLLQRLLQEHRLKTQSELDRINAQYLEVKCG 273

Query: 337 TMNAKLSVLEHVLLLETYTQESIPALHKIRKYLVEATEEASISYNKAVTRLREYQGVDPH 396
            M  KL + E  +L +TYT E +     IR  L  A          +   L  Y+ +   
Sbjct: 274 AMILKLRMEELKILSDTYTVEKVEVHRLIRDRLEGAIHLQEQDMENSRQVLNSYEVLGEE 333

Query: 397 FDTIARQYHDIVMKLENMQWTIHQ 420
           FD + ++Y  +    EN +W + +
Sbjct: 334 FDRLVKEYTVLKQATENKRWALQE 357

BLAST of MS008133 vs. ExPASy TrEMBL
Match: A0A6J1CCQ9 (AUGMIN subunit 4 OS=Momordica charantia OX=3673 GN=LOC111010312 PE=4 SV=1)

HSP 1 Score: 823.2 bits (2125), Expect = 5.2e-235
Identity = 430/433 (99.31%), Postives = 431/433 (99.54%), Query Frame = 0

Query: 1   MVKSLQGGGQNLPADVTQVIDQLERHCLAPDGSLVSKPSHYDLQLAREEMSRERLRYLEA 60
           MVKSLQGGGQNLPADVTQVIDQLERHCLAPDGSLVSKPSHYDLQLAREEMSRERLRYLEA
Sbjct: 1   MVKSLQGGGQNLPADVTQVIDQLERHCLAPDGSLVSKPSHYDLQLAREEMSRERLRYLEA 60

Query: 61  MAIYCEAIAMVEEYQQAVSMANLGGVRDVQALYPQLGLKNSPQVYETLEHRMVVAEASQR 120
           MA+YCEAIAMVEEYQQAVSMANLGG RDVQALYPQLGLKNSPQVYETLEHRMVVAEASQR
Sbjct: 61  MAVYCEAIAMVEEYQQAVSMANLGGFRDVQALYPQLGLKNSPQVYETLEHRMVVAEASQR 120

Query: 121 LRLPLISKDGEIHEEEIEKLSLISRSSLDSTSTSVTISSSTNSTNYASANSTGSIVNNIL 180
           LRLPLISKDGEIHEEEIEKLSLISRSSLDSTSTSVTISSSTNSTNYASANSTGSIVNNIL
Sbjct: 121 LRLPLISKDGEIHEEEIEKLSLISRSSLDSTSTSVTISSSTNSTNYASANSTGSIVNNIL 180

Query: 181 SVSSTDTAEPGVGGVPNRFLGITPAFLWQTQLHQTPSMDMTEYQMALSREIEARLKTKCD 240
           SVSSTDTAEPGVGGVPN FLGITPAFLWQTQLHQTPSMDMTEYQMALSREIEARLKTKCD
Sbjct: 181 SVSSTDTAEPGVGGVPNSFLGITPAFLWQTQLHQTPSMDMTEYQMALSREIEARLKTKCD 240

Query: 241 KVADAFVMDDIESSSGHHSSSARLPERVKLIIEEIEREEAALRQELYSADRKFAEYYNVL 300
           KVADAFVMDDIESSSGHHSSSARLPERVKLIIEEIEREEAALRQELYSADRKFAEYYNVL
Sbjct: 241 KVADAFVMDDIESSSGHHSSSARLPERVKLIIEEIEREEAALRQELYSADRKFAEYYNVL 300

Query: 301 EQILGVLIKLVKDLKLQHQHKYDDLQKTWLCKRCETMNAKLSVLEHVLLLETYTQESIPA 360
           EQILGVLIKLVKDLKLQHQHKYDDLQKTWLCKRCETMNAKLSVLEHVLLLETYTQESIPA
Sbjct: 301 EQILGVLIKLVKDLKLQHQHKYDDLQKTWLCKRCETMNAKLSVLEHVLLLETYTQESIPA 360

Query: 361 LHKIRKYLVEATEEASISYNKAVTRLREYQGVDPHFDTIARQYHDIVMKLENMQWTIHQV 420
           LHKIRKYLVEATEEASISYNKAVTRLREYQGVDPHFDTIARQYHDIVMKLENMQWTIHQV
Sbjct: 361 LHKIRKYLVEATEEASISYNKAVTRLREYQGVDPHFDTIARQYHDIVMKLENMQWTIHQV 420

Query: 421 EMDLKRLPDQSST 434
           EMDLKRLPDQSST
Sbjct: 421 EMDLKRLPDQSST 433

BLAST of MS008133 vs. ExPASy TrEMBL
Match: A0A1S3BHI2 (AUGMIN subunit 4 OS=Cucumis melo OX=3656 GN=LOC103490141 PE=4 SV=1)

HSP 1 Score: 803.9 bits (2075), Expect = 3.3e-229
Identity = 421/433 (97.23%), Postives = 426/433 (98.38%), Query Frame = 0

Query: 1   MVKSLQGGGQNLPADVTQVIDQLERHCLAPDGSLVSKPSHYDLQLAREEMSRERLRYLEA 60
           MVKSLQGGGQNLPADVTQVIDQLERHCLAPDGSLVSKP+HYDLQLAREEMSRERLRYLEA
Sbjct: 1   MVKSLQGGGQNLPADVTQVIDQLERHCLAPDGSLVSKPAHYDLQLAREEMSRERLRYLEA 60

Query: 61  MAIYCEAIAMVEEYQQAVSMANLGGVRDVQALYPQLGLKNSPQVYETLEHRMVVAEASQR 120
           MAIYCEAIAMVEEYQQAVSMANLGGVRDVQALYPQLGLKNSPQVYETLEHRMVVAEASQR
Sbjct: 61  MAIYCEAIAMVEEYQQAVSMANLGGVRDVQALYPQLGLKNSPQVYETLEHRMVVAEASQR 120

Query: 121 LRLPLISKDGEIHEEEIEKLSLISRSSLDSTSTSVTISSSTNSTNYASANSTGSIVNNIL 180
           LRLPLISKDGEIHEEEIEKL   SRSSLDSTSTSVTISSSTNSTNYASA+STGSIVNN L
Sbjct: 121 LRLPLISKDGEIHEEEIEKL---SRSSLDSTSTSVTISSSTNSTNYASASSTGSIVNNSL 180

Query: 181 SVSSTDTAEPGVGGVPNRFLGITPAFLWQTQLHQTPSMDMTEYQMALSREIEARLKTKCD 240
           SVSSTDTAEPGVGGVPNRFLGITPAFLWQTQLHQTPS DM EYQMALSREI++RLKTKCD
Sbjct: 181 SVSSTDTAEPGVGGVPNRFLGITPAFLWQTQLHQTPSTDMAEYQMALSREIDSRLKTKCD 240

Query: 241 KVADAFVMDDIESSSGHHSSSARLPERVKLIIEEIEREEAALRQELYSADRKFAEYYNVL 300
           KVADAF+MDDIESSSGHHSSSARLPERVKLIIEEIEREEAALRQELYSADRKFAEYYNVL
Sbjct: 241 KVADAFIMDDIESSSGHHSSSARLPERVKLIIEEIEREEAALRQELYSADRKFAEYYNVL 300

Query: 301 EQILGVLIKLVKDLKLQHQHKYDDLQKTWLCKRCETMNAKLSVLEHVLLLETYTQESIPA 360
           EQILGVLIKLVKDLKLQHQHKYDDLQKTWLCKRCETMNAKLSVLEHVLLLETYTQESIPA
Sbjct: 301 EQILGVLIKLVKDLKLQHQHKYDDLQKTWLCKRCETMNAKLSVLEHVLLLETYTQESIPA 360

Query: 361 LHKIRKYLVEATEEASISYNKAVTRLREYQGVDPHFDTIARQYHDIVMKLENMQWTIHQV 420
           LHKIRKYLVEATEEASISYNKAVTRLREYQGVDPHFD IARQYHDIVMKLENMQWTIHQV
Sbjct: 361 LHKIRKYLVEATEEASISYNKAVTRLREYQGVDPHFDNIARQYHDIVMKLENMQWTIHQV 420

Query: 421 EMDLKRLPDQSST 434
           EMDLKRLPDQSST
Sbjct: 421 EMDLKRLPDQSST 430

BLAST of MS008133 vs. ExPASy TrEMBL
Match: A0A0A0K3S8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G210080 PE=4 SV=1)

HSP 1 Score: 800.8 bits (2067), Expect = 2.8e-228
Identity = 419/433 (96.77%), Postives = 424/433 (97.92%), Query Frame = 0

Query: 1   MVKSLQGGGQNLPADVTQVIDQLERHCLAPDGSLVSKPSHYDLQLAREEMSRERLRYLEA 60
           MVKSLQGGGQNLP DVTQVIDQLERHCLAPDGSLVSKP+HYDLQLAREEMSRERLRYLEA
Sbjct: 1   MVKSLQGGGQNLPTDVTQVIDQLERHCLAPDGSLVSKPAHYDLQLAREEMSRERLRYLEA 60

Query: 61  MAIYCEAIAMVEEYQQAVSMANLGGVRDVQALYPQLGLKNSPQVYETLEHRMVVAEASQR 120
           MAIYCEAIAMVEEYQQAVSMANLGGVRDVQALYPQLGLKNSPQVYETLEHRMVVAEASQR
Sbjct: 61  MAIYCEAIAMVEEYQQAVSMANLGGVRDVQALYPQLGLKNSPQVYETLEHRMVVAEASQR 120

Query: 121 LRLPLISKDGEIHEEEIEKLSLISRSSLDSTSTSVTISSSTNSTNYASANSTGSIVNNIL 180
           LRLPLISKDGEIHEEEIEKL   SRSSLDSTST VTISSSTNSTNYASA+STGSIVNN L
Sbjct: 121 LRLPLISKDGEIHEEEIEKL---SRSSLDSTSTGVTISSSTNSTNYASASSTGSIVNNSL 180

Query: 181 SVSSTDTAEPGVGGVPNRFLGITPAFLWQTQLHQTPSMDMTEYQMALSREIEARLKTKCD 240
           SVSSTDTAEPGVGGVPNRFLGITPAFLWQTQLH TPS DM EYQMALSREI+ARLKTKCD
Sbjct: 181 SVSSTDTAEPGVGGVPNRFLGITPAFLWQTQLHHTPSTDMAEYQMALSREIDARLKTKCD 240

Query: 241 KVADAFVMDDIESSSGHHSSSARLPERVKLIIEEIEREEAALRQELYSADRKFAEYYNVL 300
           KVADAF+MDDIESSSGHHSSSARLPERVKLIIEEIEREEAALRQELYSADRKFAEYYNVL
Sbjct: 241 KVADAFIMDDIESSSGHHSSSARLPERVKLIIEEIEREEAALRQELYSADRKFAEYYNVL 300

Query: 301 EQILGVLIKLVKDLKLQHQHKYDDLQKTWLCKRCETMNAKLSVLEHVLLLETYTQESIPA 360
           EQILGVLIKLVKDLKLQHQHKYDDLQKTWLCKRCETMNAKLSVLEHVLLLETYTQESIPA
Sbjct: 301 EQILGVLIKLVKDLKLQHQHKYDDLQKTWLCKRCETMNAKLSVLEHVLLLETYTQESIPA 360

Query: 361 LHKIRKYLVEATEEASISYNKAVTRLREYQGVDPHFDTIARQYHDIVMKLENMQWTIHQV 420
           LHKIRKYLVEATEEASISYNKAVTRLREYQGVDPHFDTIARQYHDIVMKL+NMQWTIHQV
Sbjct: 361 LHKIRKYLVEATEEASISYNKAVTRLREYQGVDPHFDTIARQYHDIVMKLDNMQWTIHQV 420

Query: 421 EMDLKRLPDQSST 434
           EMDLKRLPDQSST
Sbjct: 421 EMDLKRLPDQSST 430

BLAST of MS008133 vs. ExPASy TrEMBL
Match: A0A5A7SKF9 (AUGMIN subunit 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold142G001020 PE=4 SV=1)

HSP 1 Score: 798.9 bits (2062), Expect = 1.1e-227
Identity = 419/433 (96.77%), Postives = 424/433 (97.92%), Query Frame = 0

Query: 1   MVKSLQGGGQNLPADVTQVIDQLERHCLAPDGSLVSKPSHYDLQLAREEMSRERLRYLEA 60
           MVKSLQGGGQNLPADVTQVIDQLERHCLAPDGSLVSKP+HYDLQLAREEMSRERLRYLEA
Sbjct: 1   MVKSLQGGGQNLPADVTQVIDQLERHCLAPDGSLVSKPAHYDLQLAREEMSRERLRYLEA 60

Query: 61  MAIYCEAIAMVEEYQQAVSMANLGGVRDVQALYPQLGLKNSPQVYETLEHRMVVAEASQR 120
           MAIYCEAIAMVEEYQQAVSMANLGGVRDVQALYPQLGLKNSPQVYETLEHRMVVAEASQ 
Sbjct: 61  MAIYCEAIAMVEEYQQAVSMANLGGVRDVQALYPQLGLKNSPQVYETLEHRMVVAEASQG 120

Query: 121 LRLPLISKDGEIHEEEIEKLSLISRSSLDSTSTSVTISSSTNSTNYASANSTGSIVNNIL 180
           LRLPLISKDGEIHEEEIEKL   SRSSLDSTSTSVTI SSTNSTNYASA+STGSIVNN L
Sbjct: 121 LRLPLISKDGEIHEEEIEKL---SRSSLDSTSTSVTIISSTNSTNYASASSTGSIVNNSL 180

Query: 181 SVSSTDTAEPGVGGVPNRFLGITPAFLWQTQLHQTPSMDMTEYQMALSREIEARLKTKCD 240
           SVSSTDTAEPGVGGVPNRFLGITPAFLWQTQLHQTPS DM EYQMALSREI++RLKTKCD
Sbjct: 181 SVSSTDTAEPGVGGVPNRFLGITPAFLWQTQLHQTPSTDMAEYQMALSREIDSRLKTKCD 240

Query: 241 KVADAFVMDDIESSSGHHSSSARLPERVKLIIEEIEREEAALRQELYSADRKFAEYYNVL 300
           KVADAF+MDDIESSSGHHSSSARLPERVKLIIEEIEREEAALRQELYSADRKFAEYYNVL
Sbjct: 241 KVADAFIMDDIESSSGHHSSSARLPERVKLIIEEIEREEAALRQELYSADRKFAEYYNVL 300

Query: 301 EQILGVLIKLVKDLKLQHQHKYDDLQKTWLCKRCETMNAKLSVLEHVLLLETYTQESIPA 360
           EQILGVLIKLVKDLKLQHQHKYDDLQKTWLCKRCETMNAKLSVLEHVLLLETYTQESIPA
Sbjct: 301 EQILGVLIKLVKDLKLQHQHKYDDLQKTWLCKRCETMNAKLSVLEHVLLLETYTQESIPA 360

Query: 361 LHKIRKYLVEATEEASISYNKAVTRLREYQGVDPHFDTIARQYHDIVMKLENMQWTIHQV 420
           LHKIRKYLVEATEEASISYNKAVTRLREYQGVDPHFD IARQYHDIVMKLENMQWTIHQV
Sbjct: 361 LHKIRKYLVEATEEASISYNKAVTRLREYQGVDPHFDNIARQYHDIVMKLENMQWTIHQV 420

Query: 421 EMDLKRLPDQSST 434
           EMDLKRLPDQSST
Sbjct: 421 EMDLKRLPDQSST 430

BLAST of MS008133 vs. ExPASy TrEMBL
Match: A0A6J1FPS4 (AUGMIN subunit 4 OS=Cucurbita moschata OX=3662 GN=LOC111445870 PE=4 SV=1)

HSP 1 Score: 787.7 bits (2033), Expect = 2.4e-224
Identity = 413/433 (95.38%), Postives = 418/433 (96.54%), Query Frame = 0

Query: 1   MVKSLQGGGQNLPADVTQVIDQLERHCLAPDGSLVSKPSHYDLQLAREEMSRERLRYLEA 60
           M KSLQGGGQNLPADVTQVIDQLERHCLAPDGSLVSKP HYDL LAREEMSRERLRYLEA
Sbjct: 1   MAKSLQGGGQNLPADVTQVIDQLERHCLAPDGSLVSKPVHYDLLLAREEMSRERLRYLEA 60

Query: 61  MAIYCEAIAMVEEYQQAVSMANLGGVRDVQALYPQLGLKNSPQVYETLEHRMVVAEASQR 120
           MAIY EAIAMVEEYQQAVSMANLGGVRD QALYPQLGLKNS QVYETLEHRMVVAEASQR
Sbjct: 61  MAIYSEAIAMVEEYQQAVSMANLGGVRDGQALYPQLGLKNSAQVYETLEHRMVVAEASQR 120

Query: 121 LRLPLISKDGEIHEEEIEKLSLISRSSLDSTSTSVTISSSTNSTNYASANSTGSIVNNIL 180
           LRLPLISKDGEIHEEEIEKLSL+SRSSLDSTST VTISSS+NSTNYASANS GSIVNN L
Sbjct: 121 LRLPLISKDGEIHEEEIEKLSLVSRSSLDSTSTGVTISSSSNSTNYASANSAGSIVNNSL 180

Query: 181 SVSSTDTAEPGVGGVPNRFLGITPAFLWQTQLHQTPSMDMTEYQMALSREIEARLKTKCD 240
           SVSS D AEPGVGGVPNRFLGITPAFLWQTQLHQ PSMDM EYQMALSREI+ARLK KCD
Sbjct: 181 SVSSNDNAEPGVGGVPNRFLGITPAFLWQTQLHQVPSMDMPEYQMALSREIDARLKIKCD 240

Query: 241 KVADAFVMDDIESSSGHHSSSARLPERVKLIIEEIEREEAALRQELYSADRKFAEYYNVL 300
           KVADAFVMDDIESSSGHHSSSARLPERVKLIIEEIEREE ALRQ+LYSADRKFAEYYNVL
Sbjct: 241 KVADAFVMDDIESSSGHHSSSARLPERVKLIIEEIEREEEALRQDLYSADRKFAEYYNVL 300

Query: 301 EQILGVLIKLVKDLKLQHQHKYDDLQKTWLCKRCETMNAKLSVLEHVLLLETYTQESIPA 360
           EQILGVLIKLVKDLKL+HQHKYDDLQKTWLCKRCETMNAKLSVLEHVLLLETYTQESIPA
Sbjct: 301 EQILGVLIKLVKDLKLRHQHKYDDLQKTWLCKRCETMNAKLSVLEHVLLLETYTQESIPA 360

Query: 361 LHKIRKYLVEATEEASISYNKAVTRLREYQGVDPHFDTIARQYHDIVMKLENMQWTIHQV 420
           LHKIRKYLVEATEEASISYNKAVTRLREYQGVDPHFDTIARQYHDIVMKLENMQWTIHQV
Sbjct: 361 LHKIRKYLVEATEEASISYNKAVTRLREYQGVDPHFDTIARQYHDIVMKLENMQWTIHQV 420

Query: 421 EMDLKRLPDQSST 434
           EMDLKRLPDQSST
Sbjct: 421 EMDLKRLPDQSST 433

BLAST of MS008133 vs. TAIR 10
Match: AT1G50710.1 (unknown protein; Has 109 Blast hits to 109 proteins in 44 species: Archae - 0; Bacteria - 2; Metazoa - 61; Fungi - 8; Plants - 36; Viruses - 0; Other Eukaryotes - 2 (source: NCBI BLink). )

HSP 1 Score: 637.9 bits (1644), Expect = 6.0e-183
Identity = 338/425 (79.53%), Postives = 378/425 (88.94%), Query Frame = 0

Query: 1   MVKSLQGGGQNLPADVTQVIDQLERHCLAPDGSLVSKPSHYDLQLAREEMSRERLRYLEA 60
           MVK+LQG  QNLPADV Q+IDQLERHCLAPDGSLV+K  + DLQLAREEMSRERLRYLEA
Sbjct: 1   MVKALQGAAQNLPADVNQLIDQLERHCLAPDGSLVTKSVYSDLQLAREEMSRERLRYLEA 60

Query: 61  MAIYCEAIAMVEEYQQAVSMANLGGVRDVQALYPQLGLKNSPQVYETLEHRMVVAEASQR 120
           MAIYCEA+AMVEEYQQA+S+AN GG+RDVQ LYPQLGLKNSPQVYETLEHR+VVAEA+Q+
Sbjct: 61  MAIYCEAVAMVEEYQQAISVANHGGIRDVQGLYPQLGLKNSPQVYETLEHRLVVAEAAQK 120

Query: 121 LRLPLISKDGEIHEEEIEKLSLISRSSLDSTSTSVTISSSTNSTNYASANSTGSIVNNIL 180
           LRLPLIS  GEIHEEEIEK S++SRSSLDS STS TISS++NS NY  ANS+ + V   +
Sbjct: 121 LRLPLISDGGEIHEEEIEKWSILSRSSLDSASTSFTISSTSNSVNY--ANSSANSVAGGI 180

Query: 181 SVSSTDTAEPGVGGVPNRFLGITPAFLWQTQLHQTPSMDMTEYQMALSREIEARLKTKCD 240
           S+S+ DT    VGGVPNRFLGITPA+L   QL  T SMDM +YQM L+REIE RLK KCD
Sbjct: 181 SLSAVDT--DVVGGVPNRFLGITPAYLSYVQLQNTISMDMADYQMFLAREIEGRLKEKCD 240

Query: 241 KVADAFVMDDIESSSGHHSSSARLPERVKLIIEEIEREEAALRQELYSADRKFAEYYNVL 300
           K+ADA ++DD +SS+G+ +SSARLPERVK IIEEIER+EAALR++LYSADRKFAEYYNVL
Sbjct: 241 KLADA-IVDDTDSSTGNRNSSARLPERVKFIIEEIERDEAALREDLYSADRKFAEYYNVL 300

Query: 301 EQILGVLIKLVKDLKLQHQHKYDDLQKTWLCKRCETMNAKLSVLEHVLLLETYTQESIPA 360
           EQILGVLIKLVKDLKL+HQHKY+++QKTWLCKRCETMNAKL VLE+VLLLETYT +SI A
Sbjct: 301 EQILGVLIKLVKDLKLEHQHKYNEMQKTWLCKRCETMNAKLRVLENVLLLETYTPDSISA 360

Query: 361 LHKIRKYLVEATEEASISYNKAVTRLREYQGVDPHFDTIARQYHDIVMKLENMQWTIHQV 420
           LH IR YLVEATEEAS +YNKAVTRLREYQGVDPHFDTIARQYHDIV KLENMQWTIHQV
Sbjct: 361 LHNIRNYLVEATEEASAAYNKAVTRLREYQGVDPHFDTIARQYHDIVKKLENMQWTIHQV 420

Query: 421 EMDLK 426
           EMDLK
Sbjct: 421 EMDLK 420

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022139359.11.4e-23499.08AUGMIN subunit 4 [Momordica charantia][more]
XP_008447737.16.8e-22997.23PREDICTED: AUGMIN subunit 4 [Cucumis melo][more]
KAA0025153.12.2e-22796.77AUGMIN subunit 4 [Cucumis melo var. makuwa] >TYK23111.1 AUGMIN subunit 4 [Cucumi... [more]
XP_031744847.14.9e-22796.53AUGMIN subunit 4 [Cucumis sativus] >KGN44473.2 hypothetical protein Csa_016636 [... [more]
XP_038900104.11.4e-22696.07AUGMIN subunit 4 isoform X1 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
Q8GYM38.4e-18279.53AUGMIN subunit 4 OS=Arabidopsis thaliana OX=3702 GN=AUG4 PE=1 SV=1[more]
Q9H6D72.8e-0723.04HAUS augmin-like complex subunit 4 OS=Homo sapiens OX=9606 GN=HAUS4 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1CCQ95.2e-23599.31AUGMIN subunit 4 OS=Momordica charantia OX=3673 GN=LOC111010312 PE=4 SV=1[more]
A0A1S3BHI23.3e-22997.23AUGMIN subunit 4 OS=Cucumis melo OX=3656 GN=LOC103490141 PE=4 SV=1[more]
A0A0A0K3S82.8e-22896.77Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G210080 PE=4 SV=1[more]
A0A5A7SKF91.1e-22796.77AUGMIN subunit 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold142G001... [more]
A0A6J1FPS42.4e-22495.38AUGMIN subunit 4 OS=Cucurbita moschata OX=3662 GN=LOC111445870 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT1G50710.16.0e-18379.53unknown protein; Has 109 Blast hits to 109 proteins in 44 species: Archae - 0; B... [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 272..292
NoneNo IPR availablePANTHERPTHR16219:SF2BNAA06G02620D PROTEINcoord: 1..425
IPR029327HAUS augmin-like complex subunit 4PFAMPF14735HAUS4coord: 188..422
e-value: 6.3E-82
score: 274.8
IPR029327HAUS augmin-like complex subunit 4PANTHERPTHR16219AUGMIN SUBUNIT 4 FAMILY MEMBERcoord: 1..425

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS008133.1MS008133.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0051225 spindle assembly
cellular_component GO:0070652 HAUS complex