MS007731 (gene) Bitter gourd (TR) v1

BLAST of MS007731 vs. NCBI nr
Match: XP_023547636.1 (uncharacterized protein LOC111806520 [Cucurbita pepo subsp. pepo] >XP_023547637.1 uncharacterized protein LOC111806520 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 184.5 bits (467), Expect = 5.8e-43
Identity = 106/138 (76.81%), Postives = 111/138 (80.43%), Query Frame = 0

Query: 3   MSVHSR---TMLPPGASRKRKEVESFMKPLAPKAGGGGGDGDSVSSNRLLAGYLAHEFLS 62
           MSVHSR   TMLPP ASRKRKEVE F+K   PKA G     DSVSSN+LLAGYLAHEFLS
Sbjct: 1   MSVHSRTITTMLPPRASRKRKEVEPFVK---PKATG----VDSVSSNQLLAGYLAHEFLS 60

Query: 63  KGTLFGEKYEPARSEAVSLA----GECKRTKPE----AAPSIKNENQSYAEVASILKMDG 122
           KGTLFGEKYEPARSEAV +      ECKRTKPE    AAPS++ EN SYAEVASILKMDG
Sbjct: 61  KGTLFGEKYEPARSEAVGMTSSQPSECKRTKPEAAAAAAPSVRKENHSYAEVASILKMDG 120

Query: 123 AHLPGIVNPAQLARWIKM 130
           AHLPGIVNPAQLARWIKM
Sbjct: 121 AHLPGIVNPAQLARWIKM 131

BLAST of MS007731 vs. NCBI nr
Match: XP_022964143.1 (uncharacterized protein LOC111464257 [Cucurbita moschata])

HSP 1 Score: 183.3 bits (464), Expect = 1.3e-42
Identity = 103/135 (76.30%), Postives = 110/135 (81.48%), Query Frame = 0

Query: 4   SVHSR---TMLPPGASRKRKEVESFMKPLAPKAGGGGGDGDSVSSNRLLAGYLAHEFLSK 63
           SVHSR   TMLPPGASRKRKEVE+F+KP A  A       DSV SNRLLAGYLAHEFLSK
Sbjct: 3   SVHSRTTTTMLPPGASRKRKEVEAFVKPKAVGA-------DSVVSNRLLAGYLAHEFLSK 62

Query: 64  GTLFGEKYEPARSEAVSLA----GECKRTKPE--AAPSIKNENQSYAEVASILKMDGAHL 123
           GTLFGEKYEPARSEAV +      E K+TKPE  AAPS++ ENQSYAEVASILKM+GAHL
Sbjct: 63  GTLFGEKYEPARSEAVGMTCSRPAEYKKTKPEAAAAPSVEKENQSYAEVASILKMEGAHL 122

Query: 124 PGIVNPAQLARWIKM 130
           PGIVNPAQLARWIKM
Sbjct: 123 PGIVNPAQLARWIKM 130

BLAST of MS007731 vs. NCBI nr
Match: KAG6593829.1 (hypothetical protein SDJN03_13305, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 183.3 bits (464), Expect = 1.3e-42
Identity = 103/135 (76.30%), Postives = 110/135 (81.48%), Query Frame = 0

Query: 4   SVHSR---TMLPPGASRKRKEVESFMKPLAPKAGGGGGDGDSVSSNRLLAGYLAHEFLSK 63
           SVHSR   TMLPPGASRKRKEVE+F+KP A  A       DSV SNRLLAGYLAHEFLSK
Sbjct: 3   SVHSRTTTTMLPPGASRKRKEVETFVKPKAVGA-------DSVVSNRLLAGYLAHEFLSK 62

Query: 64  GTLFGEKYEPARSEAVSLA----GECKRTKPE--AAPSIKNENQSYAEVASILKMDGAHL 123
           GTLFGEKYEPARSEAV +      E K+TKPE  AAPS++ ENQSYAEVASILKM+GAHL
Sbjct: 63  GTLFGEKYEPARSEAVGMTCSRPAEYKKTKPEAAAAPSVEKENQSYAEVASILKMEGAHL 122

Query: 124 PGIVNPAQLARWIKM 130
           PGIVNPAQLARWIKM
Sbjct: 123 PGIVNPAQLARWIKM 130

BLAST of MS007731 vs. NCBI nr
Match: KAG6574904.1 (hypothetical protein SDJN03_25543, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 183.0 bits (463), Expect = 1.7e-42
Identity = 107/140 (76.43%), Postives = 111/140 (79.29%), Query Frame = 0

Query: 3   MSVHSR---TMLPPGASRKRKEVESFMKPLAPKAGGGGGDGDSVSSNRLLAGYLAHEFLS 62
           MSVHSR   TMLPP ASRKRKEVE F+K   PKA G     DSVSSN+LLAGYLAHEFLS
Sbjct: 1   MSVHSRTITTMLPPRASRKRKEVEPFVK---PKATG----VDSVSSNQLLAGYLAHEFLS 60

Query: 63  KGTLFGEKYEPARSEAV----SLAGECKRTKPE------AAPSIKNENQSYAEVASILKM 122
           KGTLFGEKYEPARSEAV    S   ECKR KPE      AAPS++ ENQSYAEVASILKM
Sbjct: 61  KGTLFGEKYEPARSEAVRMTSSQPSECKRMKPEAATAAAAAPSVRKENQSYAEVASILKM 120

Query: 123 DGAHLPGIVNPAQLARWIKM 130
           DGAHLPGIVNPAQLARWIKM
Sbjct: 121 DGAHLPGIVNPAQLARWIKM 133

BLAST of MS007731 vs. NCBI nr
Match: KAG7026157.1 (hypothetical protein SDJN02_12656, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 181.8 bits (460), Expect = 3.8e-42
Identity = 102/135 (75.56%), Postives = 110/135 (81.48%), Query Frame = 0

Query: 4   SVHSR---TMLPPGASRKRKEVESFMKPLAPKAGGGGGDGDSVSSNRLLAGYLAHEFLSK 63
           SVHSR   TMLPPGASRKRKEVE+F+KP A  A       DSV SNRLLAGYLAHEFLSK
Sbjct: 3   SVHSRTTTTMLPPGASRKRKEVETFVKPKAVGA-------DSVVSNRLLAGYLAHEFLSK 62

Query: 64  GTLFGEKYEPARSEAVSLA----GECKRTKPE--AAPSIKNENQSYAEVASILKMDGAHL 123
           GTLFGEKYEPARSEAV +      E K+TKPE  AAPS++ ENQSYAEVASILKM+GAHL
Sbjct: 63  GTLFGEKYEPARSEAVGMTCSRPAEYKKTKPEAAAAPSVEKENQSYAEVASILKMEGAHL 122

Query: 124 PGIVNPAQLARWIKM 130
           PGIVNPAQLARWIK+
Sbjct: 123 PGIVNPAQLARWIKI 130

BLAST of MS007731 vs. ExPASy TrEMBL
Match: A0A6J1HJY7 (uncharacterized protein LOC111464257 OS=Cucurbita moschata OX=3662 GN=LOC111464257 PE=4 SV=1)

HSP 1 Score: 183.3 bits (464), Expect = 6.3e-43
Identity = 103/135 (76.30%), Postives = 110/135 (81.48%), Query Frame = 0

Query: 4   SVHSR---TMLPPGASRKRKEVESFMKPLAPKAGGGGGDGDSVSSNRLLAGYLAHEFLSK 63
           SVHSR   TMLPPGASRKRKEVE+F+KP A  A       DSV SNRLLAGYLAHEFLSK
Sbjct: 3   SVHSRTTTTMLPPGASRKRKEVEAFVKPKAVGA-------DSVVSNRLLAGYLAHEFLSK 62

Query: 64  GTLFGEKYEPARSEAVSLA----GECKRTKPE--AAPSIKNENQSYAEVASILKMDGAHL 123
           GTLFGEKYEPARSEAV +      E K+TKPE  AAPS++ ENQSYAEVASILKM+GAHL
Sbjct: 63  GTLFGEKYEPARSEAVGMTCSRPAEYKKTKPEAAAAPSVEKENQSYAEVASILKMEGAHL 122

Query: 124 PGIVNPAQLARWIKM 130
           PGIVNPAQLARWIKM
Sbjct: 123 PGIVNPAQLARWIKM 130

BLAST of MS007731 vs. ExPASy TrEMBL
Match: A0A6J1KXG9 (uncharacterized protein LOC111499092 OS=Cucurbita maxima OX=3661 GN=LOC111499092 PE=4 SV=1)

HSP 1 Score: 181.4 bits (459), Expect = 2.4e-42
Identity = 104/136 (76.47%), Postives = 109/136 (80.15%), Query Frame = 0

Query: 3   MSVHSR---TMLPPGASRKRKEVESFMKPLAPKAGGGGGDGDSVSSNRLLAGYLAHEFLS 62
           MS HSR   TMLPP ASRKRKEVE F+K   PKA G     DSVSSN+LLAGYLAHEFLS
Sbjct: 1   MSFHSRTITTMLPPRASRKRKEVEPFVK---PKATG----VDSVSSNQLLAGYLAHEFLS 60

Query: 63  KGTLFGEKYEPARSEAVSLA----GECKRTKPE--AAPSIKNENQSYAEVASILKMDGAH 122
           KGTLFGEKYEP RSEAV +      ECKRTKPE  AAPS++ EN SYAEVASILKMDGAH
Sbjct: 61  KGTLFGEKYEPPRSEAVGMTSSQPSECKRTKPEAAAAPSVRKENHSYAEVASILKMDGAH 120

Query: 123 LPGIVNPAQLARWIKM 130
           LPGIVNPAQLARWIKM
Sbjct: 121 LPGIVNPAQLARWIKM 129

BLAST of MS007731 vs. ExPASy TrEMBL
Match: A0A6J1H3W4 (uncharacterized protein LOC111460190 OS=Cucurbita moschata OX=3662 GN=LOC111460190 PE=4 SV=1)

HSP 1 Score: 180.3 bits (456), Expect = 5.3e-42
Identity = 102/142 (71.83%), Postives = 108/142 (76.06%), Query Frame = 0

Query: 3   MSVHSR---TMLPPGASRKRKEVESFMKPLAPKAGGGGGDGDSVSSNRLLAGYLAHEFLS 62
           MSVHSR   TMLPP ASRKRKEVE F+KP A          DSVSSN+LLAGYLAHEFLS
Sbjct: 1   MSVHSRTITTMLPPRASRKRKEVEPFVKPKATAV-------DSVSSNQLLAGYLAHEFLS 60

Query: 63  KGTLFGEKYEPARSEAVSLA----GECKRTKPE--------AAPSIKNENQSYAEVASIL 122
           KGTLFGEKYEPARSEAV +      ECKR KPE        AAPS++ E+ SYAEVASIL
Sbjct: 61  KGTLFGEKYEPARSEAVGMTSSQPSECKRMKPEAATAAAAAAAPSVRKEDHSYAEVASIL 120

Query: 123 KMDGAHLPGIVNPAQLARWIKM 130
           KMDGAHLPGIVNPAQLARWIKM
Sbjct: 121 KMDGAHLPGIVNPAQLARWIKM 135

BLAST of MS007731 vs. ExPASy TrEMBL
Match: A0A5A7UAS9 (Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold772G00360 PE=4 SV=1)

HSP 1 Score: 154.1 bits (388), Expect = 4.1e-34
Identity = 87/126 (69.05%), Postives = 96/126 (76.19%), Query Frame = 0

Query: 10  MLPPGAS-RKRKEVESFMKPLAPKAGGGGGDGDSVSSNRLLAGYLAHEFLSKGTLFGEKY 69
           M PPG S RKRKEVE  +KP   +A       D++S+NRLLAGYLAHEFLS GTLFGEKY
Sbjct: 1   MSPPGGSPRKRKEVEPLVKPKVAEA-------DAISANRLLAGYLAHEFLSNGTLFGEKY 60

Query: 70  EPARSEAVSLA----GECKRTKPEAAPS-IKNENQSYAEVASILKMDGAHLPGIVNPAQL 129
           E A++EAV +A     ECKRTKPEAA + IK  N SYAEVASILKMDGAHLPGIVNP QL
Sbjct: 61  ETAQTEAVGMANLQSAECKRTKPEAAAAGIKKLNPSYAEVASILKMDGAHLPGIVNPGQL 119

BLAST of MS007731 vs. ExPASy TrEMBL
Match: A0A0A0LI65 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G173040 PE=4 SV=1)

HSP 1 Score: 151.4 bits (381), Expect = 2.6e-33
Identity = 87/126 (69.05%), Postives = 93/126 (73.81%), Query Frame = 0

Query: 10  MLPPGAS-RKRKEVESFMKPLAPKAGGGGGDGDSVSSNRLLAGYLAHEFLSKGTLFGEKY 69
           M PPG S RKRKEVE  +KP   +A       DS+S+NRLLAGYLAHEFL  GTLFGEKY
Sbjct: 1   MSPPGGSPRKRKEVEPLVKPKVAEA-------DSISANRLLAGYLAHEFLCNGTLFGEKY 60

Query: 70  EPARSEAVSLAG----ECKRTKPE-AAPSIKNENQSYAEVASILKMDGAHLPGIVNPAQL 129
           EPA +EAV +A     ECKRTK E AA SIK  N SYAEVA ILKMDGAHLPGIVNP QL
Sbjct: 61  EPALNEAVGMANSQSTECKRTKLEAAAASIKKVNHSYAEVARILKMDGAHLPGIVNPGQL 119

BLAST of MS007731 vs. TAIR 10
Match: AT5G44060.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G04000.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). )

HSP 1 Score: 92.0 bits (227), Expect = 3.6e-19
Identity = 48/94 (51.06%), Postives = 69/94 (73.40%), Query Frame = 0

Query: 41  DSVSSNRLLAGYLAHEFLSKGTLFGEKYEPARSEAVSLAGEC---KRTKP--EAAPSIKN 100
           + V SN+LLAGYLAHEFL+ GTLFGE + P +++A  L  +    ++ KP  +  PS  +
Sbjct: 58  EPVCSNQLLAGYLAHEFLNNGTLFGELWNPTKAQAGPLTTQSIDPRKNKPSHDIEPS-DH 117

Query: 101 ENQSYAEVASILKMDGAHLPGIVNPAQLARWIKM 130
           + + Y EVA+IL++DG HLPGIVNP+QLAR++K+
Sbjct: 118 KRRRYVEVANILRVDGTHLPGIVNPSQLARFLKL 150

BLAST of MS007731 vs. TAIR 10
Match: AT3G23440.1 (embryo sac development arrest 6 )

HSP 1 Score: 90.5 bits (223), Expect = 1.1e-18
Identity = 49/116 (42.24%), Postives = 68/116 (58.62%), Query Frame = 0

Query: 14  GASRKRKEVESFMKPLAPKAGGGGGDGDSVSSNRLLAGYLAHEFLSKGTLFGEKYEPARS 73
           GASRKRK+ ES ++             ++ + N LLAGY+AHE+L+ GT+ G K     +
Sbjct: 10  GASRKRKDTESDLR-------------EAATPNWLLAGYMAHEYLTCGTMLGRKLYSGWA 69

Query: 74  EAVSLAGECKRTKPEAAPSIKNENQSYAEVASILKMDGAHLPGIVNPAQLARWIKM 130
           E     G      P  +  +K   QSY+EVAS+ K DG H+PG+VNP QLA+WI+M
Sbjct: 70  E----VGPLVSPSPLQSREVKKARQSYSEVASVFKTDGNHVPGVVNPTQLAKWIQM 108

BLAST of MS007731 vs. TAIR 10
Match: AT1G04000.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G44060.1); Has 62 Blast hits to 62 proteins in 10 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 62; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 84.0 bits (206), Expect = 9.9e-17
Identity = 48/109 (44.04%), Postives = 73/109 (66.97%), Query Frame = 0

Query: 26  MKPLAPKAGGGGGDGDSVSSNRL-LAGYLAHEFLSKGTLFGEKYEPARSEAVSLAGECKR 85
           + P++ K+       + + SN+L LAGYL+HE+L++GTLFGE++  AR++A     E  +
Sbjct: 51  VNPISKKSSTAA--AEPIGSNQLMLAGYLSHEYLTQGTLFGEQWNQARAQA-----ESSK 110

Query: 86  TKP----EAAPSIKNENQSYAEVASILKMDGAHLPGIVNPAQLARWIKM 130
            KP    E A   + + + Y EVA++L+ DGA LPGIVNPAQLAR++K+
Sbjct: 111 IKPSHTVEPAEECEPKRKRYREVANLLRSDGAQLPGIVNPAQLARFLKL 152