MS007728 (gene) Bitter gourd (TR) v1

BLAST of MS007728 vs. NCBI nr
Match: XP_022151923.1 (uncharacterized protein LOC111019778 [Momordica charantia])

HSP 1 Score: 171.4 bits (433), Expect = 6.3e-39
Identity = 97/157 (61.78%), Postives = 117/157 (74.52%), Query Frame = 0

Query: 4   NLVCLSLSVMLFLAAGVSVLADGSDMCTKAEHVALCRSMAGG-ASDPVAATKAGLEKLIM 63
           NLVCLSL   +FLAA ++     +D+C KA+HVALCRSMA G  ++PVAAT+AG+EKLI 
Sbjct: 5   NLVCLSL---VFLAAAMA-QTHAADICAKADHVALCRSMAKGVGNNPVAATRAGIEKLIT 64

Query: 64  ETKRAMEASVKSGGKGPLEVCKETYGYAIGDLETGLEYLKSNDKGSLGGEMSSAMTYYTT 123
            TK A EAS+KSG  G L VCKETY  AI +L+TGL+YL+  DK SL  E+S AMT YTT
Sbjct: 65  ATKLAREASIKSGRSGALGVCKETYANAISELKTGLDYLQKKDKDSLNIELSGAMTDYTT 124

Query: 124 CDDAIKESGTSGEVAGILKADSTLLHMASTCMYLASL 160
           CDDAI ESG  G+  G+LK D  LL MASTC+YLA+L
Sbjct: 125 CDDAIAESGVVGKAVGVLKNDRMLLRMASTCLYLATL 157

BLAST of MS007728 vs. NCBI nr
Match: XP_022151772.1 (uncharacterized protein LOC111019676 [Momordica charantia])

HSP 1 Score: 157.5 bits (397), Expect = 9.5e-35
Identity = 89/157 (56.69%), Postives = 111/157 (70.70%), Query Frame = 0

Query: 4   NLVCLSLSVMLFLAAGVSVLADGSDMCTKAEHVALCRSMAGGA-SDPVAATKAGLEKLIM 63
           NLVCLS    L L A     A  +D+C KA++ ALCRSM  GA ++PVAAT+AG+EKLI+
Sbjct: 5   NLVCLS----LVLVAVAVAQAHAADICAKADYAALCRSMVKGAGNNPVAATRAGIEKLIL 64

Query: 64  ETKRAMEASVKSGGKGPLEVCKETYGYAIGDLETGLEYLKSNDKGSLGGEMSSAMTYYTT 123
            TK A EA +KS   G L VCKET+  AI +L+ GL+YL+  DK SL  E+S AMT YTT
Sbjct: 65  ATKIAREARLKSAKSGALGVCKETFANAISELKMGLDYLQKKDKDSLNIELSGAMTDYTT 124

Query: 124 CDDAIKESGTSGEVAGILKADSTLLHMASTCMYLASL 160
           CDDAI ESG  G+  GI+K D TLL+MAS C+YL++L
Sbjct: 125 CDDAIAESGVVGKAVGIVKIDRTLLNMASNCLYLSTL 157

BLAST of MS007728 vs. NCBI nr
Match: XP_023551699.1 (pectinesterase 3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 138.3 bits (347), Expect = 5.9e-29
Identity = 73/149 (48.99%), Postives = 104/149 (69.80%), Query Frame = 0

Query: 12  VMLFLAAGV-SVLADGSDMCTKAEHVALCRSMAGGASDPVAATKAGLEKLIMETKRAMEA 71
           V+LF+AA V +  ADG+D+C KAE+++LCRS   GA++P A  +A +E LI+ETK A E+
Sbjct: 7   VLLFVAAAVLAGQADGADICEKAEYMSLCRSSVKGATEPAAGLQAAIEHLILETKHAKES 66

Query: 72  SVKSGGKGPLEVCKETYGYAIGDLETGLEYLKSNDKGSLGGEMSSAMTYYTTCDDAIKES 131
           S+K G    L+VCK+ Y  A+ DL+  ++YL  NDK SL   +S+A+T Y TCDDA+ ES
Sbjct: 67  SMKLGAMKCLDVCKQNYDDAVTDLQKSVQYLTKNDKPSLKISLSAALTDYVTCDDAVMES 126

Query: 132 GTSGEVAGILKADSTLLHMASTCMYLASL 160
           G S +V  ++  D  L HMA+ C++LASL
Sbjct: 127 GNSKKVGDLMSVDVVLRHMAANCLHLASL 155

BLAST of MS007728 vs. NCBI nr
Match: XP_022957327.1 (pectinesterase 3-like [Cucurbita moschata])

HSP 1 Score: 135.6 bits (340), Expect = 3.9e-28
Identity = 73/149 (48.99%), Postives = 102/149 (68.46%), Query Frame = 0

Query: 12  VMLFLAAGV-SVLADGSDMCTKAEHVALCRSMAGGASDPVAATKAGLEKLIMETKRAMEA 71
           V+LF+AA V +  ADG+D+C KAE+ +LCRS   GA++P A  +A +E LI ETK A E+
Sbjct: 7   VLLFVAAAVLAGQADGADICEKAEYASLCRSSVKGATEPAAGLQAAIEHLISETKHAKES 66

Query: 72  SVKSGGKGPLEVCKETYGYAIGDLETGLEYLKSNDKGSLGGEMSSAMTYYTTCDDAIKES 131
           S+K G    L+VCK+ Y  A+ DL+  ++YL  NDK SL   +S+A+T Y TCDDA+ ES
Sbjct: 67  SMKLGVMKCLDVCKQNYNDAVTDLQQSVQYLTKNDKPSLKISLSAALTDYVTCDDAVMES 126

Query: 132 GTSGEVAGILKADSTLLHMASTCMYLASL 160
           G S +V  ++  D  L HMA+ C++LASL
Sbjct: 127 GNSKKVGDLMSVDVMLRHMAANCLHLASL 155

BLAST of MS007728 vs. NCBI nr
Match: KAG7032299.1 (hypothetical protein SDJN02_06343, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 135.2 bits (339), Expect = 5.0e-28
Identity = 74/149 (49.66%), Postives = 102/149 (68.46%), Query Frame = 0

Query: 12  VMLFLAAGV-SVLADGSDMCTKAEHVALCRSMAGGASDPVAATKAGLEKLIMETKRAMEA 71
           V+LF+AA V +  ADG+D+C KAE+ +LCRS   GA++P A  +A +E LI ETK A E+
Sbjct: 7   VLLFVAAAVLAGQADGADICEKAEYGSLCRSSVKGATEPAAGLQAAIEHLISETKHAKES 66

Query: 72  SVKSGGKGPLEVCKETYGYAIGDLETGLEYLKSNDKGSLGGEMSSAMTYYTTCDDAIKES 131
           S+K G    L+VCK+ Y  AI DL+  ++YL  NDK SL   +S+A+T Y TCDDA+ ES
Sbjct: 67  SMKLGVMKCLDVCKQNYNDAITDLQKSVQYLTKNDKPSLKISLSAALTDYVTCDDAVMES 126

Query: 132 GTSGEVAGILKADSTLLHMASTCMYLASL 160
           G S +V  ++  D  L HMA+ C++LASL
Sbjct: 127 GNSKKVGDLMSVDVVLRHMAANCLHLASL 155

BLAST of MS007728 vs. ExPASy TrEMBL
Match: A0A6J1DDH2 (uncharacterized protein LOC111019778 OS=Momordica charantia OX=3673 GN=LOC111019778 PE=4 SV=1)

HSP 1 Score: 171.4 bits (433), Expect = 3.1e-39
Identity = 97/157 (61.78%), Postives = 117/157 (74.52%), Query Frame = 0

Query: 4   NLVCLSLSVMLFLAAGVSVLADGSDMCTKAEHVALCRSMAGG-ASDPVAATKAGLEKLIM 63
           NLVCLSL   +FLAA ++     +D+C KA+HVALCRSMA G  ++PVAAT+AG+EKLI 
Sbjct: 5   NLVCLSL---VFLAAAMA-QTHAADICAKADHVALCRSMAKGVGNNPVAATRAGIEKLIT 64

Query: 64  ETKRAMEASVKSGGKGPLEVCKETYGYAIGDLETGLEYLKSNDKGSLGGEMSSAMTYYTT 123
            TK A EAS+KSG  G L VCKETY  AI +L+TGL+YL+  DK SL  E+S AMT YTT
Sbjct: 65  ATKLAREASIKSGRSGALGVCKETYANAISELKTGLDYLQKKDKDSLNIELSGAMTDYTT 124

Query: 124 CDDAIKESGTSGEVAGILKADSTLLHMASTCMYLASL 160
           CDDAI ESG  G+  G+LK D  LL MASTC+YLA+L
Sbjct: 125 CDDAIAESGVVGKAVGVLKNDRMLLRMASTCLYLATL 157

BLAST of MS007728 vs. ExPASy TrEMBL
Match: A0A6J1DED8 (uncharacterized protein LOC111019676 OS=Momordica charantia OX=3673 GN=LOC111019676 PE=4 SV=1)

HSP 1 Score: 157.5 bits (397), Expect = 4.6e-35
Identity = 89/157 (56.69%), Postives = 111/157 (70.70%), Query Frame = 0

Query: 4   NLVCLSLSVMLFLAAGVSVLADGSDMCTKAEHVALCRSMAGGA-SDPVAATKAGLEKLIM 63
           NLVCLS    L L A     A  +D+C KA++ ALCRSM  GA ++PVAAT+AG+EKLI+
Sbjct: 5   NLVCLS----LVLVAVAVAQAHAADICAKADYAALCRSMVKGAGNNPVAATRAGIEKLIL 64

Query: 64  ETKRAMEASVKSGGKGPLEVCKETYGYAIGDLETGLEYLKSNDKGSLGGEMSSAMTYYTT 123
            TK A EA +KS   G L VCKET+  AI +L+ GL+YL+  DK SL  E+S AMT YTT
Sbjct: 65  ATKIAREARLKSAKSGALGVCKETFANAISELKMGLDYLQKKDKDSLNIELSGAMTDYTT 124

Query: 124 CDDAIKESGTSGEVAGILKADSTLLHMASTCMYLASL 160
           CDDAI ESG  G+  GI+K D TLL+MAS C+YL++L
Sbjct: 125 CDDAIAESGVVGKAVGIVKIDRTLLNMASNCLYLSTL 157

BLAST of MS007728 vs. ExPASy TrEMBL
Match: A0A6J1GYU1 (pectinesterase 3-like OS=Cucurbita moschata OX=3662 GN=LOC111458758 PE=4 SV=1)

HSP 1 Score: 135.6 bits (340), Expect = 1.9e-28
Identity = 73/149 (48.99%), Postives = 102/149 (68.46%), Query Frame = 0

Query: 12  VMLFLAAGV-SVLADGSDMCTKAEHVALCRSMAGGASDPVAATKAGLEKLIMETKRAMEA 71
           V+LF+AA V +  ADG+D+C KAE+ +LCRS   GA++P A  +A +E LI ETK A E+
Sbjct: 7   VLLFVAAAVLAGQADGADICEKAEYASLCRSSVKGATEPAAGLQAAIEHLISETKHAKES 66

Query: 72  SVKSGGKGPLEVCKETYGYAIGDLETGLEYLKSNDKGSLGGEMSSAMTYYTTCDDAIKES 131
           S+K G    L+VCK+ Y  A+ DL+  ++YL  NDK SL   +S+A+T Y TCDDA+ ES
Sbjct: 67  SMKLGVMKCLDVCKQNYNDAVTDLQQSVQYLTKNDKPSLKISLSAALTDYVTCDDAVMES 126

Query: 132 GTSGEVAGILKADSTLLHMASTCMYLASL 160
           G S +V  ++  D  L HMA+ C++LASL
Sbjct: 127 GNSKKVGDLMSVDVMLRHMAANCLHLASL 155

BLAST of MS007728 vs. ExPASy TrEMBL
Match: A0A6J1JKG0 (pectinesterase 3-like OS=Cucurbita maxima OX=3661 GN=LOC111485228 PE=4 SV=1)

HSP 1 Score: 134.4 bits (337), Expect = 4.2e-28
Identity = 73/149 (48.99%), Postives = 103/149 (69.13%), Query Frame = 0

Query: 12  VMLFLAAGV-SVLADGSDMCTKAEHVALCRSMAGGASDPVAATKAGLEKLIMETKRAMEA 71
           V+LF+AA V +  ADG+D+C K+E+ +LCRS   GA++P A  +A +E L+ ETK+A E+
Sbjct: 7   VLLFVAAAVLAGQADGTDICEKSEYASLCRSSVKGATEPAAGLQAAIEHLMSETKQAKES 66

Query: 72  SVKSGGKGPLEVCKETYGYAIGDLETGLEYLKSNDKGSLGGEMSSAMTYYTTCDDAIKES 131
           S+K G    L+VCK+ Y  A+ DLE  L+YL  NDK SL   +S+A+T Y TCDDA+ ES
Sbjct: 67  SMKLGVMKCLDVCKQNYDDAVTDLEKSLQYLTINDKPSLKISLSAALTDYVTCDDAVMES 126

Query: 132 GTSGEVAGILKADSTLLHMASTCMYLASL 160
           G S +V  ++  D  L HMA+ C++LASL
Sbjct: 127 GDSKKVGDLMSIDVVLRHMAANCLHLASL 155

BLAST of MS007728 vs. ExPASy TrEMBL
Match: A0A6J1DEH0 (uncharacterized protein LOC111019774 OS=Momordica charantia OX=3673 GN=LOC111019774 PE=4 SV=1)

HSP 1 Score: 131.7 bits (330), Expect = 2.7e-27
Identity = 80/160 (50.00%), Postives = 104/160 (65.00%), Query Frame = 0

Query: 4   NLVCLSLSVMLFLAAGVSVL---ADGSDMCTKAEHVALCRSMAGGASDPVAATKAGLEKL 63
           NLVC SL   +FLAA V++L   A GSD+C  A  V  CR++  GA+DPVAA +A +E+L
Sbjct: 5   NLVCFSL---VFLAAAVALLAGHAHGSDICVNANDVPQCRAIVKGATDPVAALRAAIEQL 64

Query: 64  IMETKRAMEASVK-SGGKGPLEVCKETYGYAIGDLETGLEYLKSNDKGSLGGEMSSAMTY 123
           I ETKRA EASV  +   GPL+VCK+ +  AI DL+  LE LK  DK SL   +S+A++ 
Sbjct: 65  IFETKRANEASVALAANPGPLDVCKQNFDNAIRDLQRSLESLKIQDKDSLESHLSAALSD 124

Query: 124 YTTCDDAIKESGTSGEVAGILKADSTLLHMASTCMYLASL 160
           Y  CD A+ E     +  GI K D+ LL+MAS  +YLASL
Sbjct: 125 YVRCDGAVIEGDMMVQSVGIFKTDNVLLNMASNVIYLASL 161

BLAST of MS007728 vs. TAIR 10
Match: AT1G70540.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 72.4 bits (176), Expect = 3.7e-13
Identity = 57/166 (34.34%), Postives = 89/166 (53.61%), Query Frame = 0

Query: 4   NLVCLSLSVMLFLAAGVS-------VLADGSDMCTKAEHVALCRSMAGGASDPVAATKAG 63
           NL   +  ++L L A  S       V+  G D C+ ++   LCRS+  G  +  AAT+  
Sbjct: 4   NLHLATAVILLLLTASQSGVAMAQRVMGGGRDPCSVSDFKVLCRSVVKGQKNVNAATEVS 63

Query: 64  LEKLIMETKRAMEA---SVKSGGKGPLEVCKETYGYAIGDLETGLEYLKSNDKGSLGGEM 123
           + +L+  T +A EA   S KSG  G L+ C   Y  A+ +L+  L+ +K ND  SL   +
Sbjct: 64  IRELMKRTIKAKEAAKISRKSG--GGLKTCYSNYDSALENLQKALKNIKQNDGFSLNINL 123

Query: 124 SSAMTYYTTCDDAIKESGTSGEVAGILKADSTLLHMASTCMYLASL 160
           S+++T + TC+DA+   GT+  V    K+ STL  MA  C+ L++L
Sbjct: 124 SASLTDFDTCNDAM-GGGTASNV--FAKSTSTLHEMADNCLALSTL 164

BLAST of MS007728 vs. TAIR 10
Match: AT1G23350.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 69.7 bits (169), Expect = 2.4e-12
Identity = 52/153 (33.99%), Postives = 82/153 (53.59%), Query Frame = 0

Query: 10  LSVMLFLAAGVSVLADGS--DMCTKAEHVALCRSMAGGASDPVAATKAGLEKLIMETKRA 69
           +++ L LAA  S     S  D CT ++  ALCRS   G ++  AAT   +++L+  T++A
Sbjct: 7   VTIFLLLAATSSQALSISLADPCTVSDFPALCRSTIKGQTNVNAATDVAIKELMKRTRQA 66

Query: 70  MEASVKSGGK-GPLEVCKETYGYAIGDLETGLEYLKSNDKGSLGGEMSSAMTYYTTCDDA 129
            + + K   + G +  C   +  A  +LE  L  +K ND  SL   +S+A+T Y TC D+
Sbjct: 67  KKIAEKELKRDGGVATCLSNFNSAFDNLEKALTNIKENDGFSLNINLSAALTDYDTCSDS 126

Query: 130 IKESGTSGEVAGILKADSTLLHMASTCMYLASL 160
           +KE   + EV  I K+   L  MA  C+ L++L
Sbjct: 127 MKE---TKEVNVIYKSAGVLYRMADNCLALSTL 156

BLAST of MS007728 vs. TAIR 10
Match: AT3G36659.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 51.2 bits (121), Expect = 8.9e-07
Identity = 40/133 (30.08%), Postives = 67/133 (50.38%), Query Frame = 0

Query: 29  MCTKAEHVALCRSMAGGASDPVAA--TKAGLEKLIMETKRAMEASVKSGGKGPLEVCKET 88
           +C K ++  LC S       P     T + L   I  +  A +A++    K     C+E 
Sbjct: 133 ICGKTDNPPLCESSVSPLLTPQLKPNTSSVLILAIQASITATKAAMAIVEKVDASDCQEL 192

Query: 89  YGYAIGDLETGLEYLKSNDKGSLGGEMSSAMTYYTTCDDAIKESGTSGEVAGILKADSTL 148
           Y  A+ +LE  +  +KS+D  ++   +S+AMT Y+TC+D  +ESG    +A +  AD  L
Sbjct: 193 YDDAVVNLEDAVNAVKSSDIATVNTNLSAAMTDYSTCNDGFEESGEPNPLAYV--ADK-L 252

Query: 149 LHMASTCMYLASL 160
             M S C+ +++L
Sbjct: 253 TKMVSNCLAISTL 262