MS007672 (gene) Bitter gourd (TR) v1

Overview
NameMS007672
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionAcylamino-acid-releasing enzyme
Locationscaffold13: 330015 .. 337313 (-)
RNA-Seq ExpressionMS007672
SyntenyMS007672
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
AGTAGAAAACTGTCAACTTCCTTTGCCATGGATGGTTCAAAGATTAGTGCCGTTGTTGACGAGCTTCCAGCTGGGGTTGATCCAGAAACTGAGGGAGAATATGCTGAGCAGTCCAAATTACTTCAAGAATTCACAACAATTCCTAACATTGACAAAGCATGGATGTTTAAGTCTGACAGTGGTATGGTTGCTGTTGTTTATGCTGGTGCATTTACTATTCATGTGTTTATAATTGTCATAAAATTATGTGCTTGGCGATGTCAATTCTATTCTTACTTGACATTTTTCATAGGAGACTCCGTGGCAACGTTTTCAATTAGCCAAGCAAGTCTTCTATCAAATAAGAGGAGAAAGTATACTTTGTCAGCTCATATTTCTAAGGGAAACGACGACAGTTCCGTGAACTTTAAATGGGCTCCATTTCCTGTTGAAATGATTGGAGTCTCTACAATAGTGCCATCGCCATCAGGCTCAAAACTTCTTACAGTTCGTAATTCTGAAAATGATTCTCCAGTTAAATTGGAAATTTGGAGTGCAGGTCAAATCGAAAAGGAGTTTCACATCCCTCAATCTGTTCATGGCTCAATATATACTGATGGATGGTAATGTGTCGGTTATATTAATTTTTTGAATTGTGAATTTGATGCTAAATTAATGTTCACTTTGTAAGAATCATTTTAATATGCTTGTTCTCTTAAACTTCTAAAAAAATAATATAAAAATGGCATTCTTTATTGTATCTCCTTGGTAGAAGTTCTTTAAAATGTACTAATTTTATTGGTGGGTGAGTTTGTAGCGTCTGTCATAAAGAAAAGGCAATAAGATTTAAACTCTATTTTTTTTCTATGTTATAGGTTTGAAGGAATCTCTTGGAACTCTAATGAAACTTATATTGCTTATATTGCGGAAGAACCTTCTCCCTCCAAGCCAACTTTTACTGTTTCTGGATATCAAAAAGGTTGCTCAACAAATAAGGATTCTACCAACTGGAAAGCTCAAGGGGATTTTGAGGAAGAATGGGGGGAAGCTTATGCAGGTAAAAGGCAGCCTGCTCTCTTTGTCATCAACATTGACAGGTGTGGTTCAAGTGTTACTGTATTCATATTGACAAAATAAGGCACAATTTTAGATTTTGTGAACTTTGTTAAGAATTCCTTTTTCGTTTCTCATTAACAGCGGAGAGGTAAGGCATGTGAAATTAATCGAGAAATATTTGAGTGTGGGCCAAGTCGTATGGGCTCCATCCATAGGCGTGCATCAATATTTGGTTTTTGCTGGGTGGTCATCAGAGCCTAGAAAACTTGGTATGAAGTACTGCTATAATCGGCCTTGTGCATTATATGCAGTTAGGGCCCCGGATTATGGATCAGAAGTCGATGAACATAAACTCAAGTGTGTTATACTTTCTCTCTTATATTTTTTTTGATGGATTTTTTACTAATATCGTTTCAATCTGAACATTCTAATTTATTTCAATGTTGTCCAGAGATGGATCGAAGGAGGATTTTCCCCTCTATAATCTAACTCAGAACATAAGCAGTGCCTTCCTTCCTCGGTTCAGGTACAATTTTATCTCATTACCTTCCAGTGTTTGTTTCCCTAAACTTGTTTTTCCACAGCAAATCCAGTACGTTTTAACTTTAACTTCTGTAAATTCTTTATCAAATTTGGATGATATATATAAGTGGCTGCAGGCTATTTTCTAACGTATGTTGAGGGGAGGCTAGACTCATACTCTAATTTTGTTCTATTGTTTGCATGTGTTTTTAGTTCCAGACCTCTTTACATAAACGCTTGCGAGCATGAATATTAAATCACAACTTGGAGTACAGTTTTTATGGTCCTTGTATACTATTTAATCATTTTCATTCTTATAATTATATCTTTGTTACTTTAGAAGGTATTTCATGCTTAAAGAGTCAATTTAGATAGTTTTATTTGATGTGCATATACTTCTCCCATTGATCATTTTTTGCAAATAGTTTTTATGGGTCATCAATTAACATGCAAGCTCTATTCTATTGCATGTATATGAAATAACGCATAAGATGAGCAACGGGATTGGAACTGATTATTATGAATGATAATGTTTTCCAGCCCAGATGGGAAGTTCCTTATATTTTTATCTGCAAACTCTTCTGTGAACACTGGAGCCCATTCCGCAACAAATTCTCTTCACAGAATCAACTGGCCTACCGACGAAAAACTTTGTTTTAATGAAAATATTGTTGACGTGGTGAGCTTTGTATTTTTTATTTGTAAATATTGGTTAATGTGAAAATCTGTTGGCCGATTGATTTCTTTTAAACTAGTTTCTTCTGTGGGGTGGTGTCACTGTATATGTTACAAACATTTGGGCATGCCACATCTATCAAATACTTGTTAAGCATAATAAACATGTGTCGAATATTATGAGCATTTTGAGAATGAAAAAAATTAAAATTTTGGTTTCAAGTTTCCTTTATTTGTTAAGATGTTAAGTCATTTAGTAAATTGATTTAGTTGTTTGGTTCTTTCTAAAATATTTTGACATAAAACATTGATTATAAATGTTTAAGCCTCGTGAAGTAAAATAAATTTTTAGCATCAGAATCTTTGAAACGTCTAAAAGTAATATATCTATAAAAAATGTATATTATAATAAACTATTTTTGCTATGTCCATATCCATGTTCTAGATTTTTAGGAATGAATGTGCCACTATATCCGTGCTTCTTACGGAAGAAGCATTGGTGTTAAATCAGATGATGTTTTAATTACCGAATAGAATGTGCTTAGTTTTCTTTTGAGAGGAAACAAGAAATTTTCATTCATGTAATGAAAGGAAAGGAAAACCAGTACAGGAAAATTAAATGAAAAATAGTAAAGGTAAGAACCAAAGATATTAGCCAATAGGCGGAGTTATGAAAACCTCCCCATTCATACGAATATATTGTAAAGGAGAAGCTAGAGAGCACACCATTGGGAACCTTAAGTTTTGTCGCTTCAAAACTACCCAATGCTTGTGAGGTCTTGCGGCTTCAAAAACTTTCTTTCCATCCAAATTTCTGGGCGAATGCTTTAACTGCATATTGGTGCTTTGAAGTTATCTTATTTATTTACTAGTTATTTTTTCCAAAAGTTCCTTATGCCTTTCTTGGCAACCTTTTCTGTATAAATTAATTTTGAAGGTTCCTGTGGTGCACTGGGCTGAGGACGACCTCTTTCCTGGCCTCTATTCACATGGTTTCCTGATTAATCCATGGCTTTCTGACGGACGTACTGTTATTATATCATCCATATGGCATAGCAGTGAAGCAATACTTTCCATAGATATTTTAAGGCAATTTATCATTTGCTTTAAATTTATGTTTTAATTCTTACGCTAATCTCTGTGCTCCAATTTGTATAATACGATGATATTTTTTTATTGAACATTTCAGTGGTGAAGTATCACGCATCAGTCCATCCAACTCAAACTTTTCGTGGAATGTTCTCGCTCTAGATGGAGACAATGTTGTTGCTGGTAATGTGGAAATGAGATCTTCCTGATAAGCTTGCTCGCTGGAACTGATTTTTAACATTTTTTCTTTGAAGTTCCTATCTGCAGTTTCTAGCAGCCCAGTAGATATTCCTCAACTCAAGTACGGTCGTCTTATTGAGAAAGAAACTAAGAATGCTGTATGGAGTTGGTTAGATGTATCAAGTCCTGTCTTTAAATGCTCTGAGAAGGTTGTTCAATTTTTCATTTATAAACACCACAAATGTTATCCCAAAAGTTCTGATTAAGTAAACCGGCACTTTATGATACAGGTTAAAACTCTACTCTCAGCTCTTCAGTTCAGTATAATGAAGATTCCAGTCAAGGATGTTTCCAACTGCCTGACAAAAGGTACTGTACAGAACAGATGATTATTTACCATTTTATTTCCAATGTGCAGATTTGATTCAGACCAAATGTTGCATGAATGCATCTTCAGAATACCTATGCCTGAAGCCTATACATACTCAGACTAATGAGAGAAATTCTTAGTATTACATTGCTTTTGGTGAAACTTTCTTCACTACTGGAAGTAAACACAAAACAATCCATTCCCCATTATTTTTAATAATCAAAATTGTTGCAGTGCTGTTTGTGCAACAGAACTGAACTTCATTATTTTTTTCAATAGAAATTTCTCTCTTCAAAATTTAAAAAATAACAATCCTTTACTGTTTAACTTCATAAATAAATAGCAGTCATACAAGTCAATGAGCTAGTTAATCCAATTCTCATGAAAATTTAGATGCGATATAAAATTTTGCTGTCAACTTTCTTGATGCAATTAACTACCATATGTTTTAACGAAAATTTGAATTAATCATGAAATATAGATGATCTGGGAATGTTCCTATATTTGGTGACCTTCTCTGATGATAATGTTGGTTTCCTTTTGTCAATGTTTTGGTCAAATATAGAACATTTCTTGAGCTTATAAAATGCAAATCAAAACATGTGAGTTTTGTTTCTTCTTCTATGAAATTTTTACCCTCGAAAAAGTGATGAAAGGTAAGTGTTAGTTCAGTTACTTTCTTTTCAGGTGCTAGCAAACCTTTTGAAGCTATCTTTGTGTCTTCCAAGTCCGTCAAAGGGAATGAGTTGAATCCAGTTATTGTTAACCTTCACGGCGGTCCACATTCTACGTCCATATCAAGCTTCTCAAAGTCCTCGGCATTTCTCTCTTCACTAGGATTCAACCTTTTGGTTGTGAACTATAGGTAGGTCGACTTTCTTCCCTCTGATCTTAATTTGGGTTTAGTTGTACCTAGAAGTATTTGCAATTGAAGTTGGGTCAAATTAATCAGCTTTCAGTTGGTAATATATATAATATAATAAGCGTATATCTCACCTGGACCCCTTCTCTCCAGTTTATCTACTACTCGGGTCTTTTTTATTATTCAAAACCTCACATAAGGATTACTCCTACTTCTCGAGACAAATTTTTCGAAAAAGATTAGCTTTAATTACTGGACATAAAATTTTTTACTGAATTATTTGAGAGCCAGATTTGAAAAAGTCATTTGTTTATAAAGTGAGATCGAGGCGATTGAAAAATTATGGTTTATATATCAGATATAGTGTCACTGACTCACAACATAAAGAGCGTTCTTTCCTTTCTTTGGCTTCTTTGATATGGTTATCTCAGAGGTTCATTGGGTTTCGGTGAGGACGCATTGCAATCTCTTCCCGGAAAAGTTGGGTCCCAGGTACTAGTTTTAGTAAATTTAGGTATTCGTTTTTTTTTTCCTCGAGAATTTTCATTTTACTGCTATCGTGATGACTGTTTTTCTTCGGATGTCACTGAATAGAATATTTTTTTAATCTATTTTTTCTCAGGATGTCAATGATGTACTTACAGCTTTAGATCACATTATCGACAAGGGACTTGCTAATCCATCTAAAGTTGCAGTTCTAGGTGGTTCTCATGGAGGTTTTTTGACAACTCATTTGATTGGCCAGGTCGAGTAGTTAACACAAAATTTTAATGTTGCATTTTCTGAAAATAATTCTCATGTTCGATTAATTCTGCGTGATTGATAAAGGATTTTGTATCTGTTGATTCTTTCCATGATTTTCCCTTTTTTTTTTTTTTTAAATTTGAACTCTCCCGAGCTACACTTGAAAATATGACGGTGCACGATGCATGTTCATTTCGTGGTCCACTAATGTGGTGATATTTTGATTTGCATTTTTTCCCTATCACACCTTCTAATTGTTCTTACTCCTTTACCTAGGGTAAGTTGATATTACTTTTTCGAAGGTTAAAAAATACTTTTTGTCCAATAAGAACTCTTTTCAATAATTTCCTAGTATTTTATTAGATTATCAAAAGTGATTTTTGAAATTCTTAAAAATACTTTAAAATTTTTTCAGAATGCTTTTAAGAGAAGCAATTAATTAGTGCTTTTCAAAACAACGCTTAAATAAATCACCACTATGAAAAAAGTCACCTAAACTCACATTAATGTTGGGAGTTATGGCATATAGAGTTCTAAGAAGGCAATAATTTCCTTATAAATTGAATCCCTTCTGTTTTCTAAATGATGCAGGCACCAGATAGATTTGTGGCAGCTGCTGCAAGAAATCCTGTGTGTAACCTTGCTTTAATGGTTGGCACATCAGATATTCCTGATTGGTGTTATGTGGAATGTTACGGAAGGGAGTGGAAGAATTACTTCACGGAAGCACCATCTGCTGAGCACCAAATCGAGTTATACAACAAATCTCCCATTCTACATGTCTCAAAAGTACGTAGATCAAAATCTAACCATCTATTTCTTTATTGGTACATTGATGTTTTAAAATTCTTGCTAAACTTGGAAAAAAATAACACTCATAAAAGCTTTGGTCAATTGATCTAATAGTGCAGAGGACTAAATTTTGTTTATTTATTTTTTGTAAGAAAAATTGTAAGCTCTGTGAACTTTATTGAAGAATCCATCGTTCATTGAAGGATCTTCTTAGGGAGGATCCTCGTCTTGTGAGTAACAATATTTTTTTTTTAACTTTCTTAAAACGTGGTGAGATATGATATAAATCAATCTTAATCAGAAGATAGAACTGTTATTTAGTCCAAGTATCTTTATTTGGAGAATTATTTTACCAACAACTCTGTTTTTTTTTATATATATATTTGACCTGTTTATCAATACTGCTAGCTTCACGTATTTTCTCAGTATACCTTAATAAATTTCTTTCTTTTTGAGTGGATGTTCTTCGTTGAAATCTTCTGTCTCATTTCGATTTTGCAGGTTAAAGCACCCACCATTTTTCTTTTAGGTGCTAAAGATCTTCGTGTTCCATTTTCAAATGGATTGCAAGTAAGATGTAACACATTCTATTAAATTCTACGTCCTTTTTGACTTATTTCTATGATTTTTTATTTTTTATTTTTTTTATTTCATCATGTGCGATGGGGTTCTAACCCCCAATTTTTTGGTTGAAAATATATGCTTTACAATCAGTTGAGCTATACTCAGGTTGACTTATTTCTGTGACTATAAAATGACTGTGGTGTTGTTATGTTTATCACCAAGAATGATCGCACGAAAACAAGAAACTGTGTCCAATCTCGGACTCTTTGAATTTCTTATTATACCAGAAAATTATGTCAAAAAATACTTTAATGTTATACTTAAGATGGTTATATATAATATGCAGTTTGCACGAGCGTTGAAGGAGAAAGGAGTTGAGGTCAAAGTGATCATGTTTCCAGATGATATTCATCCCATTGACAGGTTCCACATCTACTTACTTTCGCATTCTCGCATTTGTTCTGTT

mRNA sequence

AGTAGAAAACTGTCAACTTCCTTTGCCATGGATGGTTCAAAGATTAGTGCCGTTGTTGACGAGCTTCCAGCTGGGGTTGATCCAGAAACTGAGGGAGAATATGCTGAGCAGTCCAAATTACTTCAAGAATTCACAACAATTCCTAACATTGACAAAGCATGGATGTTTAAGTCTGACAGTGGAGACTCCGTGGCAACGTTTTCAATTAGCCAAGCAAGTCTTCTATCAAATAAGAGGAGAAAGTATACTTTGTCAGCTCATATTTCTAAGGGAAACGACGACAGTTCCGTGAACTTTAAATGGGCTCCATTTCCTGTTGAAATGATTGGAGTCTCTACAATAGTGCCATCGCCATCAGGCTCAAAACTTCTTACAGTTCGTAATTCTGAAAATGATTCTCCAGTTAAATTGGAAATTTGGAGTGCAGGTCAAATCGAAAAGGAGTTTCACATCCCTCAATCTGTTCATGGCTCAATATATACTGATGGATGGTTTGAAGGAATCTCTTGGAACTCTAATGAAACTTATATTGCTTATATTGCGGAAGAACCTTCTCCCTCCAAGCCAACTTTTACTGTTTCTGGATATCAAAAAGGTTGCTCAACAAATAAGGATTCTACCAACTGGAAAGCTCAAGGGGATTTTGAGGAAGAATGGGGGGAAGCTTATGCAGGTAAAAGGCAGCCTGCTCTCTTTGTCATCAACATTGACAGCGGAGAGGTAAGGCATGTGAAATTAATCGAGAAATATTTGAGTGTGGGCCAAGTCGTATGGGCTCCATCCATAGGCGTGCATCAATATTTGGTTTTTGCTGGGTGGTCATCAGAGCCTAGAAAACTTGGTATGAAGTACTGCTATAATCGGCCTTGTGCATTATATGCAGTTAGGGCCCCGGATTATGGATCAGAAGTCGATGAACATAAACTCAAAGATGGATCGAAGGAGGATTTTCCCCTCTATAATCTAACTCAGAACATAAGCAGTGCCTTCCTTCCTCGGTTCAGCCCAGATGGGAAGTTCCTTATATTTTTATCTGCAAACTCTTCTGTGAACACTGGAGCCCATTCCGCAACAAATTCTCTTCACAGAATCAACTGGCCTACCGACGAAAAACTTTGTTTTAATGAAAATATTGTTGACGTGGTTCCTGTGGTGCACTGGGCTGAGGACGACCTCTTTCCTGGCCTCTATTCACATGGTTTCCTGATTAATCCATGGCTTTCTGACGGACGTACTGTTATTATATCATCCATATGGCATAGCAGTGAAGCAATACTTTCCATAGATATTTTAAGGCAATTTATCATTTGCTTTAAATTTATTGGTGAAGTATCACGCATCAGTCCATCCAACTCAAACTTTTCGTGGAATGTTCTCGCTCTAGATGGAGACAATGTTGTTGCTGTTTCTAGCAGCCCAGTAGATATTCCTCAACTCAAGTACGGTCGTCTTATTGAGAAAGAAACTAAGAATGCTGTATGGAGTTGGTTAGATGTATCAAGTCCTGTCTTTAAATGCTCTGAGAAGGTTGTTAAAACTCTACTCTCAGCTCTTCAGTTCAGTATAATGAAGATTCCAGTCAAGGATGTTTCCAACTGCCTGACAAAAGGTGCTAGCAAACCTTTTGAAGCTATCTTTGTGTCTTCCAAGTCCGTCAAAGGGAATGAGTTGAATCCAGTTATTGTTAACCTTCACGGCGGTCCACATTCTACGTCCATATCAAGCTTCTCAAAGTCCTCGGCATTTCTCTCTTCACTAGGATTCAACCTTTTGGTTGTGAACTATAGAGGTTCATTGGGTTTCGGTGAGGACGCATTGCAATCTCTTCCCGGAAAAGTTGGGTCCCAGGATGTCAATGATGTACTTACAGCTTTAGATCACATTATCGACAAGGGACTTGCTAATCCATCTAAAGTTGCAGTTCTAGGTGGTTCTCATGGAGGTTTTTTGACAACTCATTTGATTGGCCAGGCACCAGATAGATTTGTGGCAGCTGCTGCAAGAAATCCTGTGTGTAACCTTGCTTTAATGGTTGGCACATCAGATATTCCTGATTGGTGTTATGTGGAATGTTACGGAAGGGAGTGGAAGAATTACTTCACGGAAGCACCATCTGCTGAGCACCAAATCGAGTTATACAACAAATCTCCCATTCTACATGTCTCAAAAGTTAAAGCACCCACCATTTTTCTTTTAGGTGCTAAAGATCTTCGTGTTCCATTTTCAAATGGATTGCAATTTGCACGAGCGTTGAAGGAGAAAGGAGTTGAGGTCAAAGTGATCATGTTTCCAGATGATATTCATCCCATTGACAGGTTCCACATCTACTTACTTTCGCATTCTCGCATTTGTTCTGTT

Coding sequence (CDS)

AGTAGAAAACTGTCAACTTCCTTTGCCATGGATGGTTCAAAGATTAGTGCCGTTGTTGACGAGCTTCCAGCTGGGGTTGATCCAGAAACTGAGGGAGAATATGCTGAGCAGTCCAAATTACTTCAAGAATTCACAACAATTCCTAACATTGACAAAGCATGGATGTTTAAGTCTGACAGTGGAGACTCCGTGGCAACGTTTTCAATTAGCCAAGCAAGTCTTCTATCAAATAAGAGGAGAAAGTATACTTTGTCAGCTCATATTTCTAAGGGAAACGACGACAGTTCCGTGAACTTTAAATGGGCTCCATTTCCTGTTGAAATGATTGGAGTCTCTACAATAGTGCCATCGCCATCAGGCTCAAAACTTCTTACAGTTCGTAATTCTGAAAATGATTCTCCAGTTAAATTGGAAATTTGGAGTGCAGGTCAAATCGAAAAGGAGTTTCACATCCCTCAATCTGTTCATGGCTCAATATATACTGATGGATGGTTTGAAGGAATCTCTTGGAACTCTAATGAAACTTATATTGCTTATATTGCGGAAGAACCTTCTCCCTCCAAGCCAACTTTTACTGTTTCTGGATATCAAAAAGGTTGCTCAACAAATAAGGATTCTACCAACTGGAAAGCTCAAGGGGATTTTGAGGAAGAATGGGGGGAAGCTTATGCAGGTAAAAGGCAGCCTGCTCTCTTTGTCATCAACATTGACAGCGGAGAGGTAAGGCATGTGAAATTAATCGAGAAATATTTGAGTGTGGGCCAAGTCGTATGGGCTCCATCCATAGGCGTGCATCAATATTTGGTTTTTGCTGGGTGGTCATCAGAGCCTAGAAAACTTGGTATGAAGTACTGCTATAATCGGCCTTGTGCATTATATGCAGTTAGGGCCCCGGATTATGGATCAGAAGTCGATGAACATAAACTCAAAGATGGATCGAAGGAGGATTTTCCCCTCTATAATCTAACTCAGAACATAAGCAGTGCCTTCCTTCCTCGGTTCAGCCCAGATGGGAAGTTCCTTATATTTTTATCTGCAAACTCTTCTGTGAACACTGGAGCCCATTCCGCAACAAATTCTCTTCACAGAATCAACTGGCCTACCGACGAAAAACTTTGTTTTAATGAAAATATTGTTGACGTGGTTCCTGTGGTGCACTGGGCTGAGGACGACCTCTTTCCTGGCCTCTATTCACATGGTTTCCTGATTAATCCATGGCTTTCTGACGGACGTACTGTTATTATATCATCCATATGGCATAGCAGTGAAGCAATACTTTCCATAGATATTTTAAGGCAATTTATCATTTGCTTTAAATTTATTGGTGAAGTATCACGCATCAGTCCATCCAACTCAAACTTTTCGTGGAATGTTCTCGCTCTAGATGGAGACAATGTTGTTGCTGTTTCTAGCAGCCCAGTAGATATTCCTCAACTCAAGTACGGTCGTCTTATTGAGAAAGAAACTAAGAATGCTGTATGGAGTTGGTTAGATGTATCAAGTCCTGTCTTTAAATGCTCTGAGAAGGTTGTTAAAACTCTACTCTCAGCTCTTCAGTTCAGTATAATGAAGATTCCAGTCAAGGATGTTTCCAACTGCCTGACAAAAGGTGCTAGCAAACCTTTTGAAGCTATCTTTGTGTCTTCCAAGTCCGTCAAAGGGAATGAGTTGAATCCAGTTATTGTTAACCTTCACGGCGGTCCACATTCTACGTCCATATCAAGCTTCTCAAAGTCCTCGGCATTTCTCTCTTCACTAGGATTCAACCTTTTGGTTGTGAACTATAGAGGTTCATTGGGTTTCGGTGAGGACGCATTGCAATCTCTTCCCGGAAAAGTTGGGTCCCAGGATGTCAATGATGTACTTACAGCTTTAGATCACATTATCGACAAGGGACTTGCTAATCCATCTAAAGTTGCAGTTCTAGGTGGTTCTCATGGAGGTTTTTTGACAACTCATTTGATTGGCCAGGCACCAGATAGATTTGTGGCAGCTGCTGCAAGAAATCCTGTGTGTAACCTTGCTTTAATGGTTGGCACATCAGATATTCCTGATTGGTGTTATGTGGAATGTTACGGAAGGGAGTGGAAGAATTACTTCACGGAAGCACCATCTGCTGAGCACCAAATCGAGTTATACAACAAATCTCCCATTCTACATGTCTCAAAAGTTAAAGCACCCACCATTTTTCTTTTAGGTGCTAAAGATCTTCGTGTTCCATTTTCAAATGGATTGCAATTTGCACGAGCGTTGAAGGAGAAAGGAGTTGAGGTCAAAGTGATCATGTTTCCAGATGATATTCATCCCATTGACAGGTTCCACATCTACTTACTTTCGCATTCTCGCATTTGTTCTGTT

Protein sequence

SRKLSTSFAMDGSKISAVVDELPAGVDPETEGEYAEQSKLLQEFTTIPNIDKAWMFKSDSGDSVATFSISQASLLSNKRRKYTLSAHISKGNDDSSVNFKWAPFPVEMIGVSTIVPSPSGSKLLTVRNSENDSPVKLEIWSAGQIEKEFHIPQSVHGSIYTDGWFEGISWNSNETYIAYIAEEPSPSKPTFTVSGYQKGCSTNKDSTNWKAQGDFEEEWGEAYAGKRQPALFVINIDSGEVRHVKLIEKYLSVGQVVWAPSIGVHQYLVFAGWSSEPRKLGMKYCYNRPCALYAVRAPDYGSEVDEHKLKDGSKEDFPLYNLTQNISSAFLPRFSPDGKFLIFLSANSSVNTGAHSATNSLHRINWPTDEKLCFNENIVDVVPVVHWAEDDLFPGLYSHGFLINPWLSDGRTVIISSIWHSSEAILSIDILRQFIICFKFIGEVSRISPSNSNFSWNVLALDGDNVVAVSSSPVDIPQLKYGRLIEKETKNAVWSWLDVSSPVFKCSEKVVKTLLSALQFSIMKIPVKDVSNCLTKGASKPFEAIFVSSKSVKGNELNPVIVNLHGGPHSTSISSFSKSSAFLSSLGFNLLVVNYRGSLGFGEDALQSLPGKVGSQDVNDVLTALDHIIDKGLANPSKVAVLGGSHGGFLTTHLIGQAPDRFVAAAARNPVCNLALMVGTSDIPDWCYVECYGREWKNYFTEAPSAEHQIELYNKSPILHVSKVKAPTIFLLGAKDLRVPFSNGLQFARALKEKGVEVKVIMFPDDIHPIDRFHIYLLSHSRICSV
Homology
BLAST of MS007672 vs. NCBI nr
Match: XP_022138558.1 (acylamino-acid-releasing enzyme isoform X2 [Momordica charantia])

HSP 1 Score: 1523.1 bits (3942), Expect = 0.0e+00
Identity = 757/772 (98.06%), Postives = 758/772 (98.19%), Query Frame = 0

Query: 1   SRKLSTSFAMDGSKISAVVDELPAGVDPETEGEYAEQSKLLQEFTTIPNIDKAWMFKSDS 60
           +RKLSTSFAMDGSKISAVVDELPAGVDPETEGEYAEQSKLLQEFTTIPNIDKAWMFKSDS
Sbjct: 42  TRKLSTSFAMDGSKISAVVDELPAGVDPETEGEYAEQSKLLQEFTTIPNIDKAWMFKSDS 101

Query: 61  GDSVATFSISQASLLSNKRRKYTLSAHISKGNDDSSVNFKWAPFPVEMIGVSTIVPSPSG 120
           GDSVATFSISQASLLSNKRRKYTLSAHISKGN DSSVNFKWAPFPVEMIGVSTIVPSPSG
Sbjct: 102 GDSVATFSISQASLLSNKRRKYTLSAHISKGNGDSSVNFKWAPFPVEMIGVSTIVPSPSG 161

Query: 121 SKLLTVRNSENDSPVKLEIWSAGQIEKEFHIPQSVHGSIYTDGWFEGISWNSNETYIAYI 180
           SKLLTVRNSENDSPVKLEIWSAGQIEKEFHIPQSVHGSIYTDGWFEGISWNSNETYIAYI
Sbjct: 162 SKLLTVRNSENDSPVKLEIWSAGQIEKEFHIPQSVHGSIYTDGWFEGISWNSNETYIAYI 221

Query: 181 AEEPSPSKPTFTVSGYQKGCSTNKDSTNWKAQGDFEEEWGEAYAGKRQPALFVINIDSGE 240
           AEEPSPSKPTFTVSGYQKGCSTNKDSTNWKAQGDFEEEWGEAYAGKRQPALFVINIDSGE
Sbjct: 222 AEEPSPSKPTFTVSGYQKGCSTNKDSTNWKAQGDFEEEWGEAYAGKRQPALFVINIDSGE 281

Query: 241 VRHVKLIEKYLSVGQVVWAPSIGVHQYLVFAGWSSEPRKLGMKYCYNRPCALYAVRAPDY 300
           VRHVKLIEKYLSVGQVVWAPSIGVHQYLVFAGWSSEPRKLGMKYCYNRPCALYAVRAPDY
Sbjct: 282 VRHVKLIEKYLSVGQVVWAPSIGVHQYLVFAGWSSEPRKLGMKYCYNRPCALYAVRAPDY 341

Query: 301 GSEVDEHKLKDGSKEDFPLYNLTQNISSAFLPRFSPDGKFLIFLSANSSVNTGAHSATNS 360
           GSEVDEHKLKDGSKEDFPLYNLTQNISSAFLPRFSPDGKFLIFLSANSSVNTGAHSATNS
Sbjct: 342 GSEVDEHKLKDGSKEDFPLYNLTQNISSAFLPRFSPDGKFLIFLSANSSVNTGAHSATNS 401

Query: 361 LHRINWPTDEKLCFNENIVDVVPVVHWAEDDLFPGLYSHGFLINPWLSDGRTVIISSIWH 420
           LHRINWPTDEKLCFNENIVDVVPVVHWAEDDLFPGLYSHGFLINPWLSDGRTVIISSIWH
Sbjct: 402 LHRINWPTDEKLCFNENIVDVVPVVHWAEDDLFPGLYSHGFLINPWLSDGRTVIISSIWH 461

Query: 421 SSEAILSIDILRQFIICFKFIGEVSRISPSNSNFSWNVLALDGDNVVAVSSSPVDIPQLK 480
           SSEAILSIDIL          GEVSRISPSNSNFSWNVLALDGDNVVAVSSSPVDIPQLK
Sbjct: 462 SSEAILSIDILS---------GEVSRISPSNSNFSWNVLALDGDNVVAVSSSPVDIPQLK 521

Query: 481 YGRLIEKETKNAVWSWLDVSSPVFKCSEKVVKTLLSALQFSIMKIPVKDVSNCLTKGASK 540
           YGRLIEKETKNAVWSWLDVSSPVFKCSEK VKTLLSALQFSIMKIPVKDVSNCLTKGASK
Sbjct: 522 YGRLIEKETKNAVWSWLDVSSPVFKCSEK-VKTLLSALQFSIMKIPVKDVSNCLTKGASK 581

Query: 541 PFEAIFVSSKSVKGNELNPVIVNLHGGPHSTSISSFSKSSAFLSSLGFNLLVVNYRGSLG 600
           PFEAIFVSSKSVKGNELNPVIVNLHGGPHSTSISSFSKSSAFLSSLGFNLLVVNYRGSLG
Sbjct: 582 PFEAIFVSSKSVKGNELNPVIVNLHGGPHSTSISSFSKSSAFLSSLGFNLLVVNYRGSLG 641

Query: 601 FGEDALQSLPGKVGSQDVNDVLTALDHIIDKGLANPSKVAVLGGSHGGFLTTHLIGQAPD 660
           FGEDALQSLPGKVGSQDVNDVL ALDHIIDKGLANPSKVAVLGGSHGGFLTTHLIGQAPD
Sbjct: 642 FGEDALQSLPGKVGSQDVNDVLAALDHIIDKGLANPSKVAVLGGSHGGFLTTHLIGQAPD 701

Query: 661 RFVAAAARNPVCNLALMVGTSDIPDWCYVECYGREWKNYFTEAPSAEHQIELYNKSPILH 720
           RFVAAAARNPVCNLALMVGTSDIPDWCYVECYGRE KNYFTEAPSAEHQIELYNKSPILH
Sbjct: 702 RFVAAAARNPVCNLALMVGTSDIPDWCYVECYGREGKNYFTEAPSAEHQIELYNKSPILH 761

Query: 721 VSKVKAPTIFLLGAKDLRVPFSNGLQFARALKEKGVEVKVIMFPDDIHPIDR 773
           VSKVKAPTIFLLGAKDLRVPFSNGLQFARALKEKGVEVKVIMFPDDIHPIDR
Sbjct: 762 VSKVKAPTIFLLGAKDLRVPFSNGLQFARALKEKGVEVKVIMFPDDIHPIDR 803

BLAST of MS007672 vs. NCBI nr
Match: XP_022138557.1 (acylamino-acid-releasing enzyme isoform X1 [Momordica charantia])

HSP 1 Score: 1516.9 bits (3926), Expect = 0.0e+00
Identity = 757/777 (97.43%), Postives = 758/777 (97.55%), Query Frame = 0

Query: 1   SRKLSTSFAMDGSKISAVVDELPAGVDPETEGEYAEQSKLLQEFTTIPNIDKAWMFKSDS 60
           +RKLSTSFAMDGSKISAVVDELPAGVDPETEGEYAEQSKLLQEFTTIPNIDKAWMFKSDS
Sbjct: 42  TRKLSTSFAMDGSKISAVVDELPAGVDPETEGEYAEQSKLLQEFTTIPNIDKAWMFKSDS 101

Query: 61  GDSVATFSISQASLLSNKRRKYTLSAHISKGNDDSSVNFKWAPFPVEMIGVSTIVPSPSG 120
           GDSVATFSISQASLLSNKRRKYTLSAHISKGN DSSVNFKWAPFPVEMIGVSTIVPSPSG
Sbjct: 102 GDSVATFSISQASLLSNKRRKYTLSAHISKGNGDSSVNFKWAPFPVEMIGVSTIVPSPSG 161

Query: 121 SKLLTVRNSENDSPVKLEIWSAGQIEKEFHIPQSVHGSIYTDGWFEGISWNSNETYIAYI 180
           SKLLTVRNSENDSPVKLEIWSAGQIEKEFHIPQSVHGSIYTDGWFEGISWNSNETYIAYI
Sbjct: 162 SKLLTVRNSENDSPVKLEIWSAGQIEKEFHIPQSVHGSIYTDGWFEGISWNSNETYIAYI 221

Query: 181 AEEPSPSKPTFTVSGYQKGCSTNKDSTNWKAQGDFEEEWGEAYAGKRQPALFVINIDSGE 240
           AEEPSPSKPTFTVSGYQKGCSTNKDSTNWKAQGDFEEEWGEAYAGKRQPALFVINIDSGE
Sbjct: 222 AEEPSPSKPTFTVSGYQKGCSTNKDSTNWKAQGDFEEEWGEAYAGKRQPALFVINIDSGE 281

Query: 241 VRHVKLIEKYLSVGQVVWAPSIGVHQYLVFAGWSSEPRKLGMKYCYNRPCALYAVRAPDY 300
           VRHVKLIEKYLSVGQVVWAPSIGVHQYLVFAGWSSEPRKLGMKYCYNRPCALYAVRAPDY
Sbjct: 282 VRHVKLIEKYLSVGQVVWAPSIGVHQYLVFAGWSSEPRKLGMKYCYNRPCALYAVRAPDY 341

Query: 301 GSEVDEHKLKDGSKEDFPLYNLTQNISSAFLPRFSPDGKFLIFLSANSSVNTGAHSATNS 360
           GSEVDEHKLKDGSKEDFPLYNLTQNISSAFLPRFSPDGKFLIFLSANSSVNTGAHSATNS
Sbjct: 342 GSEVDEHKLKDGSKEDFPLYNLTQNISSAFLPRFSPDGKFLIFLSANSSVNTGAHSATNS 401

Query: 361 LHRINWPTDEKLCFNENIVDVVPVVHWAEDDLFPGLYSHGFLINPWLSDGRTVIISSIWH 420
           LHRINWPTDEKLCFNENIVDVVPVVHWAEDDLFPGLYSHGFLINPWLSDGRTVIISSIWH
Sbjct: 402 LHRINWPTDEKLCFNENIVDVVPVVHWAEDDLFPGLYSHGFLINPWLSDGRTVIISSIWH 461

Query: 421 SSEAILSIDILRQFIICFKFIGEVSRISPSNSNFSWNVLALDGDNVVAVSSSPVDIPQLK 480
           SSEAILSIDIL          GEVSRISPSNSNFSWNVLALDGDNVVAVSSSPVDIPQLK
Sbjct: 462 SSEAILSIDILS---------GEVSRISPSNSNFSWNVLALDGDNVVAVSSSPVDIPQLK 521

Query: 481 YGRLIEKETKNAVWSWLDVSSPVFKCSEKVVKTLLSALQFSIMKIPVKDVSNCLTK---- 540
           YGRLIEKETKNAVWSWLDVSSPVFKCSEK VKTLLSALQFSIMKIPVKDVSNCLTK    
Sbjct: 522 YGRLIEKETKNAVWSWLDVSSPVFKCSEK-VKTLLSALQFSIMKIPVKDVSNCLTKVTFF 581

Query: 541 -GASKPFEAIFVSSKSVKGNELNPVIVNLHGGPHSTSISSFSKSSAFLSSLGFNLLVVNY 600
            GASKPFEAIFVSSKSVKGNELNPVIVNLHGGPHSTSISSFSKSSAFLSSLGFNLLVVNY
Sbjct: 582 SGASKPFEAIFVSSKSVKGNELNPVIVNLHGGPHSTSISSFSKSSAFLSSLGFNLLVVNY 641

Query: 601 RGSLGFGEDALQSLPGKVGSQDVNDVLTALDHIIDKGLANPSKVAVLGGSHGGFLTTHLI 660
           RGSLGFGEDALQSLPGKVGSQDVNDVL ALDHIIDKGLANPSKVAVLGGSHGGFLTTHLI
Sbjct: 642 RGSLGFGEDALQSLPGKVGSQDVNDVLAALDHIIDKGLANPSKVAVLGGSHGGFLTTHLI 701

Query: 661 GQAPDRFVAAAARNPVCNLALMVGTSDIPDWCYVECYGREWKNYFTEAPSAEHQIELYNK 720
           GQAPDRFVAAAARNPVCNLALMVGTSDIPDWCYVECYGRE KNYFTEAPSAEHQIELYNK
Sbjct: 702 GQAPDRFVAAAARNPVCNLALMVGTSDIPDWCYVECYGREGKNYFTEAPSAEHQIELYNK 761

Query: 721 SPILHVSKVKAPTIFLLGAKDLRVPFSNGLQFARALKEKGVEVKVIMFPDDIHPIDR 773
           SPILHVSKVKAPTIFLLGAKDLRVPFSNGLQFARALKEKGVEVKVIMFPDDIHPIDR
Sbjct: 762 SPILHVSKVKAPTIFLLGAKDLRVPFSNGLQFARALKEKGVEVKVIMFPDDIHPIDR 808

BLAST of MS007672 vs. NCBI nr
Match: XP_022138559.1 (acylamino-acid-releasing enzyme isoform X3 [Momordica charantia])

HSP 1 Score: 1502.6 bits (3889), Expect = 0.0e+00
Identity = 749/768 (97.53%), Postives = 749/768 (97.53%), Query Frame = 0

Query: 10  MDGSKISAVVDELPAGVDPETEGEYAEQSKLLQEFTTIPNIDKAWMFKSDSGDSVATFSI 69
           MDGSKISAVVDELPAGVDPETEGEYAEQSKLLQEFTTIPNIDKAWMFKSDSGDSVATFSI
Sbjct: 1   MDGSKISAVVDELPAGVDPETEGEYAEQSKLLQEFTTIPNIDKAWMFKSDSGDSVATFSI 60

Query: 70  SQASLLSNKRRKYTLSAHISKGNDDSSVNFKWAPFPVEMIGVSTIVPSPSGSKLLTVRNS 129
           SQASLLSNKRRKYTLSAHISKGN DSSVNFKWAPFPVEMIGVSTIVPSPSGSKLLTVRNS
Sbjct: 61  SQASLLSNKRRKYTLSAHISKGNGDSSVNFKWAPFPVEMIGVSTIVPSPSGSKLLTVRNS 120

Query: 130 ENDSPVKLEIWSAGQIEKEFHIPQSVHGSIYTDGWFEGISWNSNETYIAYIAEEPSPSKP 189
           ENDSPVKLEIWSAGQIEKEFHIPQSVHGSIYTDGWFEGISWNSNETYIAYIAEEPSPSKP
Sbjct: 121 ENDSPVKLEIWSAGQIEKEFHIPQSVHGSIYTDGWFEGISWNSNETYIAYIAEEPSPSKP 180

Query: 190 TFTVSGYQKGCSTNKDSTNWKAQGDFEEEWGEAYAGKRQPALFVINIDSGEVRHVKLIEK 249
           TFTVSGYQKGCSTNKDSTNWKAQGDFEEEWGEAYAGKRQPALFVINIDSGEVRHVKLIEK
Sbjct: 181 TFTVSGYQKGCSTNKDSTNWKAQGDFEEEWGEAYAGKRQPALFVINIDSGEVRHVKLIEK 240

Query: 250 YLSVGQVVWAPSIGVHQYLVFAGWSSEPRKLGMKYCYNRPCALYAVRAPDYGSEVDEHKL 309
           YLSVGQVVWAPSIGVHQYLVFAGWSSEPRKLGMKYCYNRPCALYAVRAPDYGSEVDEHKL
Sbjct: 241 YLSVGQVVWAPSIGVHQYLVFAGWSSEPRKLGMKYCYNRPCALYAVRAPDYGSEVDEHKL 300

Query: 310 KDGSKEDFPLYNLTQNISSAFLPRFSPDGKFLIFLSANSSVNTGAHSATNSLHRINWPTD 369
           KDGSKEDFPLYNLTQNISSAFLPRFSPDGKFLIFLSANSSVNTGAHSATNSLHRINWPTD
Sbjct: 301 KDGSKEDFPLYNLTQNISSAFLPRFSPDGKFLIFLSANSSVNTGAHSATNSLHRINWPTD 360

Query: 370 EKLCFNENIVDVVPVVHWAEDDLFPGLYSHGFLINPWLSDGRTVIISSIWHSSEAILSID 429
           EKLCFNENIVDVVPVVHWAEDDLFPGLYSHGFLINPWLSDGRTVIISSIWHSSEAILSID
Sbjct: 361 EKLCFNENIVDVVPVVHWAEDDLFPGLYSHGFLINPWLSDGRTVIISSIWHSSEAILSID 420

Query: 430 ILRQFIICFKFIGEVSRISPSNSNFSWNVLALDGDNVVAVSSSPVDIPQLKYGRLIEKET 489
           IL          GEVSRISPSNSNFSWNVLALDGDNVVAVSSSPVDIPQLKYGRLIEKET
Sbjct: 421 ILS---------GEVSRISPSNSNFSWNVLALDGDNVVAVSSSPVDIPQLKYGRLIEKET 480

Query: 490 KNAVWSWLDVSSPVFKCSEKVVKTLLSALQFSIMKIPVKDVSNCLTK-----GASKPFEA 549
           KNAVWSWLDVSSPVFKCSEK VKTLLSALQFSIMKIPVKDVSNCLTK     GASKPFEA
Sbjct: 481 KNAVWSWLDVSSPVFKCSEK-VKTLLSALQFSIMKIPVKDVSNCLTKVTFFSGASKPFEA 540

Query: 550 IFVSSKSVKGNELNPVIVNLHGGPHSTSISSFSKSSAFLSSLGFNLLVVNYRGSLGFGED 609
           IFVSSKSVKGNELNPVIVNLHGGPHSTSISSFSKSSAFLSSLGFNLLVVNYRGSLGFGED
Sbjct: 541 IFVSSKSVKGNELNPVIVNLHGGPHSTSISSFSKSSAFLSSLGFNLLVVNYRGSLGFGED 600

Query: 610 ALQSLPGKVGSQDVNDVLTALDHIIDKGLANPSKVAVLGGSHGGFLTTHLIGQAPDRFVA 669
           ALQSLPGKVGSQDVNDVL ALDHIIDKGLANPSKVAVLGGSHGGFLTTHLIGQAPDRFVA
Sbjct: 601 ALQSLPGKVGSQDVNDVLAALDHIIDKGLANPSKVAVLGGSHGGFLTTHLIGQAPDRFVA 660

Query: 670 AAARNPVCNLALMVGTSDIPDWCYVECYGREWKNYFTEAPSAEHQIELYNKSPILHVSKV 729
           AAARNPVCNLALMVGTSDIPDWCYVECYGRE KNYFTEAPSAEHQIELYNKSPILHVSKV
Sbjct: 661 AAARNPVCNLALMVGTSDIPDWCYVECYGREGKNYFTEAPSAEHQIELYNKSPILHVSKV 720

Query: 730 KAPTIFLLGAKDLRVPFSNGLQFARALKEKGVEVKVIMFPDDIHPIDR 773
           KAPTIFLLGAKDLRVPFSNGLQFARALKEKGVEVKVIMFPDDIHPIDR
Sbjct: 721 KAPTIFLLGAKDLRVPFSNGLQFARALKEKGVEVKVIMFPDDIHPIDR 758

BLAST of MS007672 vs. NCBI nr
Match: XP_038875186.1 (acylamino-acid-releasing enzyme isoform X1 [Benincasa hispida])

HSP 1 Score: 1370.5 bits (3546), Expect = 0.0e+00
Identity = 674/772 (87.31%), Postives = 707/772 (91.58%), Query Frame = 0

Query: 1   SRKLSTSFAMDGSKISAVVDELPAGVDPETEGEYAEQSKLLQEFTTIPNIDKAWMFKSDS 60
           SRKLS +  MD SKIS VV+E P+G+DP TE EYAEQSKLLQEFT IPNIDKAW FKSDS
Sbjct: 42  SRKLSAALVMDASKISNVVEEFPSGIDPITEEEYAEQSKLLQEFTKIPNIDKAWTFKSDS 101

Query: 61  GDSVATFSISQASLLSNKRRKYTLSAHISKGNDDSSVNFKWAPFPVEMIGVSTIVPSPSG 120
           GDS+ATFSISQASLL+NKRRKYTLSAHISKGND +SVNF W PFPVEMIGVSTIVPSPSG
Sbjct: 102 GDSMATFSISQASLLANKRRKYTLSAHISKGNDSNSVNFTWTPFPVEMIGVSTIVPSPSG 161

Query: 121 SKLLTVRNSENDSPVKLEIWSAGQIEKEFHIPQSVHGSIYTDGWFEGISWNSNETYIAYI 180
           SK L VRN ENDSPV+LEIWSAGQIEKEFHIPQS+HGSIYTDGWFEGISWNSNETYIAY+
Sbjct: 162 SKFLAVRNPENDSPVQLEIWSAGQIEKEFHIPQSIHGSIYTDGWFEGISWNSNETYIAYV 221

Query: 181 AEEPSPSKPTFTVSGYQKGCSTNKDSTNWKAQGDFEEEWGEAYAGKRQPALFVINIDSGE 240
           AEEPSPSKPTFTVSGYQKGCSTNKDSTNWK QGDF+E+WGEAYAGKRQPALFVIN+DSGE
Sbjct: 222 AEEPSPSKPTFTVSGYQKGCSTNKDSTNWKGQGDFKEDWGEAYAGKRQPALFVINVDSGE 281

Query: 241 VRHVKLIEKYLSVGQVVWAPSIGVHQYLVFAGWSSEPRKLGMKYCYNRPCALYAVRAPDY 300
           VR VK+IE  LSVGQVVWAPSIG  QYLVF GWSS+PRKLGM YCYNRPCALYAV+APDY
Sbjct: 282 VRQVKIIEHSLSVGQVVWAPSIGEDQYLVFVGWSSDPRKLGMIYCYNRPCALYAVKAPDY 341

Query: 301 GSEVDEHKLKDGSKEDFPLYNLTQNISSAFLPRFSPDGKFLIFLSANSSVNTGAHSATNS 360
           GSEV+EHKLKD SK+DFPLYNLTQNISSAF PRFSPDGKFL+FLSA+SSVN+GAHSATNS
Sbjct: 342 GSEVNEHKLKDESKKDFPLYNLTQNISSAFFPRFSPDGKFLVFLSAHSSVNSGAHSATNS 401

Query: 361 LHRINWPTDEKLCFNENIVDVVPVVHWAEDDLFPGLYSHGFLINPWLSDGRTVIISSIWH 420
           LHRINWP D +L F ENIVDVVPVVHWAE+DLFPGLYSHGFL NPW SDG TVI +SIW 
Sbjct: 402 LHRINWPRDGQLSFRENIVDVVPVVHWAENDLFPGLYSHGFLANPWFSDGHTVITTSIWR 461

Query: 421 SSEAILSIDILRQFIICFKFIGEVSRISPSNSNFSWNVLALDGDNVVAVSSSPVDIPQLK 480
           S +AILSID L          GEVS ISP+NSNFSW VLALDGDN+VAVSSSPVDIPQLK
Sbjct: 462 SKDAILSIDTLS---------GEVSCISPANSNFSWKVLALDGDNIVAVSSSPVDIPQLK 521

Query: 481 YGRLIEKETKNAVWSWLDVSSPVFKCSEKVVKTLLSALQFSIMKIPVKDVSNCLTKGASK 540
           YG LIEKETKNA WSWLDVSSPVFKCSEK VKTLLS+LQFSIMKIPVKDVSNCLTKG+  
Sbjct: 522 YGCLIEKETKNAAWSWLDVSSPVFKCSEK-VKTLLSSLQFSIMKIPVKDVSNCLTKGSRD 581

Query: 541 PFEAIFVSSKSVKGNELNPVIVNLHGGPHSTSISSFSKSSAFLSSLGFNLLVVNYRGSLG 600
           PFEAIFVSSKSVKGNELNPVIV LHGGPHSTSISSFSKS AFLSS+GFNLLVVNYRGSLG
Sbjct: 582 PFEAIFVSSKSVKGNELNPVIVTLHGGPHSTSISSFSKSLAFLSSIGFNLLVVNYRGSLG 641

Query: 601 FGEDALQSLPGKVGSQDVNDVLTALDHIIDKGLANPSKVAVLGGSHGGFLTTHLIGQAPD 660
           FGE+ALQSLPGK+GSQDVNDVLTALDHIIDKGLAN SKV VLGGSHGGFLTTHLIGQAPD
Sbjct: 642 FGEEALQSLPGKIGSQDVNDVLTALDHIIDKGLANSSKVVVLGGSHGGFLTTHLIGQAPD 701

Query: 661 RFVAAAARNPVCNLALMVGTSDIPDWCYVECYGREWKNYFTEAPSAEHQIELYNKSPILH 720
           RFVAAAARNPVCNLALMV TSDIPDWCYVECYG E KNYFTEAPSAEH I LYNKSPI H
Sbjct: 702 RFVAAAARNPVCNLALMVSTSDIPDWCYVECYGMEGKNYFTEAPSAEHLIHLYNKSPISH 761

Query: 721 VSKVKAPTIFLLGAKDLRVPFSNGLQFARALKEKGVEVKVIMFPDDIHPIDR 773
           VSKVKAPTIFLLGAKDLRVPFSNGLQ+ARALKEKGVEVKV MFPDDIHPIDR
Sbjct: 762 VSKVKAPTIFLLGAKDLRVPFSNGLQYARALKEKGVEVKVFMFPDDIHPIDR 803

BLAST of MS007672 vs. NCBI nr
Match: XP_038875189.1 (acylamino-acid-releasing enzyme isoform X2 [Benincasa hispida])

HSP 1 Score: 1367.4 bits (3538), Expect = 0.0e+00
Identity = 672/770 (87.27%), Postives = 705/770 (91.56%), Query Frame = 0

Query: 3   KLSTSFAMDGSKISAVVDELPAGVDPETEGEYAEQSKLLQEFTTIPNIDKAWMFKSDSGD 62
           KLS +  MD SKIS VV+E P+G+DP TE EYAEQSKLLQEFT IPNIDKAW FKSDSGD
Sbjct: 43  KLSAALVMDASKISNVVEEFPSGIDPITEEEYAEQSKLLQEFTKIPNIDKAWTFKSDSGD 102

Query: 63  SVATFSISQASLLSNKRRKYTLSAHISKGNDDSSVNFKWAPFPVEMIGVSTIVPSPSGSK 122
           S+ATFSISQASLL+NKRRKYTLSAHISKGND +SVNF W PFPVEMIGVSTIVPSPSGSK
Sbjct: 103 SMATFSISQASLLANKRRKYTLSAHISKGNDSNSVNFTWTPFPVEMIGVSTIVPSPSGSK 162

Query: 123 LLTVRNSENDSPVKLEIWSAGQIEKEFHIPQSVHGSIYTDGWFEGISWNSNETYIAYIAE 182
            L VRN ENDSPV+LEIWSAGQIEKEFHIPQS+HGSIYTDGWFEGISWNSNETYIAY+AE
Sbjct: 163 FLAVRNPENDSPVQLEIWSAGQIEKEFHIPQSIHGSIYTDGWFEGISWNSNETYIAYVAE 222

Query: 183 EPSPSKPTFTVSGYQKGCSTNKDSTNWKAQGDFEEEWGEAYAGKRQPALFVINIDSGEVR 242
           EPSPSKPTFTVSGYQKGCSTNKDSTNWK QGDF+E+WGEAYAGKRQPALFVIN+DSGEVR
Sbjct: 223 EPSPSKPTFTVSGYQKGCSTNKDSTNWKGQGDFKEDWGEAYAGKRQPALFVINVDSGEVR 282

Query: 243 HVKLIEKYLSVGQVVWAPSIGVHQYLVFAGWSSEPRKLGMKYCYNRPCALYAVRAPDYGS 302
            VK+IE  LSVGQVVWAPSIG  QYLVF GWSS+PRKLGM YCYNRPCALYAV+APDYGS
Sbjct: 283 QVKIIEHSLSVGQVVWAPSIGEDQYLVFVGWSSDPRKLGMIYCYNRPCALYAVKAPDYGS 342

Query: 303 EVDEHKLKDGSKEDFPLYNLTQNISSAFLPRFSPDGKFLIFLSANSSVNTGAHSATNSLH 362
           EV+EHKLKD SK+DFPLYNLTQNISSAF PRFSPDGKFL+FLSA+SSVN+GAHSATNSLH
Sbjct: 343 EVNEHKLKDESKKDFPLYNLTQNISSAFFPRFSPDGKFLVFLSAHSSVNSGAHSATNSLH 402

Query: 363 RINWPTDEKLCFNENIVDVVPVVHWAEDDLFPGLYSHGFLINPWLSDGRTVIISSIWHSS 422
           RINWP D +L F ENIVDVVPVVHWAE+DLFPGLYSHGFL NPW SDG TVI +SIW S 
Sbjct: 403 RINWPRDGQLSFRENIVDVVPVVHWAENDLFPGLYSHGFLANPWFSDGHTVITTSIWRSK 462

Query: 423 EAILSIDILRQFIICFKFIGEVSRISPSNSNFSWNVLALDGDNVVAVSSSPVDIPQLKYG 482
           +AILSID L          GEVS ISP+NSNFSW VLALDGDN+VAVSSSPVDIPQLKYG
Sbjct: 463 DAILSIDTLS---------GEVSCISPANSNFSWKVLALDGDNIVAVSSSPVDIPQLKYG 522

Query: 483 RLIEKETKNAVWSWLDVSSPVFKCSEKVVKTLLSALQFSIMKIPVKDVSNCLTKGASKPF 542
            LIEKETKNA WSWLDVSSPVFKCSEK VKTLLS+LQFSIMKIPVKDVSNCLTKG+  PF
Sbjct: 523 CLIEKETKNAAWSWLDVSSPVFKCSEK-VKTLLSSLQFSIMKIPVKDVSNCLTKGSRDPF 582

Query: 543 EAIFVSSKSVKGNELNPVIVNLHGGPHSTSISSFSKSSAFLSSLGFNLLVVNYRGSLGFG 602
           EAIFVSSKSVKGNELNPVIV LHGGPHSTSISSFSKS AFLSS+GFNLLVVNYRGSLGFG
Sbjct: 583 EAIFVSSKSVKGNELNPVIVTLHGGPHSTSISSFSKSLAFLSSIGFNLLVVNYRGSLGFG 642

Query: 603 EDALQSLPGKVGSQDVNDVLTALDHIIDKGLANPSKVAVLGGSHGGFLTTHLIGQAPDRF 662
           E+ALQSLPGK+GSQDVNDVLTALDHIIDKGLAN SKV VLGGSHGGFLTTHLIGQAPDRF
Sbjct: 643 EEALQSLPGKIGSQDVNDVLTALDHIIDKGLANSSKVVVLGGSHGGFLTTHLIGQAPDRF 702

Query: 663 VAAAARNPVCNLALMVGTSDIPDWCYVECYGREWKNYFTEAPSAEHQIELYNKSPILHVS 722
           VAAAARNPVCNLALMV TSDIPDWCYVECYG E KNYFTEAPSAEH I LYNKSPI HVS
Sbjct: 703 VAAAARNPVCNLALMVSTSDIPDWCYVECYGMEGKNYFTEAPSAEHLIHLYNKSPISHVS 762

Query: 723 KVKAPTIFLLGAKDLRVPFSNGLQFARALKEKGVEVKVIMFPDDIHPIDR 773
           KVKAPTIFLLGAKDLRVPFSNGLQ+ARALKEKGVEVKV MFPDDIHPIDR
Sbjct: 763 KVKAPTIFLLGAKDLRVPFSNGLQYARALKEKGVEVKVFMFPDDIHPIDR 802

BLAST of MS007672 vs. ExPASy Swiss-Prot
Match: Q84LM4 (Acylamino-acid-releasing enzyme OS=Arabidopsis thaliana OX=3702 GN=AARE PE=1 SV=1)

HSP 1 Score: 932.9 bits (2410), Expect = 2.3e-270
Identity = 459/755 (60.79%), Postives = 579/755 (76.69%), Query Frame = 0

Query: 21  ELPAGVDPETEGEYAEQSKLLQEFTTIPNIDKAWMFKSDSGDSVATFSISQASLLSNKRR 80
           EL  G+DP TE EYA QSKLLQEF  IP+IDKAW+F SDSG S A F++SQA+LL+NK++
Sbjct: 11  ELHVGLDPTTEEEYATQSKLLQEFINIPSIDKAWIFNSDSG-SQAMFALSQANLLANKKK 70

Query: 81  KYTLSAHISKGNDDSSVNFKWAPFPVEMIGVSTIVPSPSGSKLLTVRNSENDSPVKLEIW 140
           K+ LS HIS    + SVNF WAPFP+EM G S  VPSPSG KLL +RN EN+SP K EIW
Sbjct: 71  KFMLSGHIS-NESNQSVNFHWAPFPIEMTGASAFVPSPSGLKLLVIRNPENESPTKFEIW 130

Query: 141 SAGQIEKEFHIPQSVHGSIYTDGWFEGISWNSNETYIAYIAEEPSPSKPTFTVSG-YQKG 200
           ++ Q+EKEFHIPQ VHGS+Y DGWFEGISW+S+ET++AY+AEEPS  KPTF   G Y+K 
Sbjct: 131 NSSQLEKEFHIPQKVHGSVYVDGWFEGISWDSDETHVAYVAEEPSRPKPTFDHLGYYKKE 190

Query: 201 CSTNKDSTNWKAQGDFEEEWGEAYAGKRQPALFVINIDSGEVRHVKLIEKYLSVGQVVWA 260
            S +K   +WK +GD+EEEWGEAYAGKRQPALFVIN+DSGEV  +K I + +SVGQVVW+
Sbjct: 191 NSLDKGIGSWKGEGDWEEEWGEAYAGKRQPALFVINVDSGEVEPIKGIPRSISVGQVVWS 250

Query: 261 P-SIGVHQYLVFAGWSSEPRKLGMKYCYNRPCALYAVRAPDYGSEVDEHKLKDGSKEDFP 320
           P S G  QYLVFAGW  + RK G+KYCYNRPCA+YA++           + KD    +FP
Sbjct: 251 PNSNGSAQYLVFAGWLGDKRKFGIKYCYNRPCAIYAIK-------FTSDEPKDDDANEFP 310

Query: 321 LYNLTQNISSAFLPRFSPDGKFLIFLSANSSVNTGAHSATNSLHRINWPTDEKLCFNENI 380
           ++NLT++ISS F PRFS DGKFL+F+SA ++V++GAH AT SLHRI+WP+D KL  + NI
Sbjct: 311 IHNLTKSISSGFCPRFSKDGKFLVFVSAKTAVDSGAHWATESLHRIDWPSDGKLPESTNI 370

Query: 381 VDVVPVVHWAEDDLFPGLYSHGFLINPWLSDGRTVIISSIWHSSEAILSIDILRQFIICF 440
           VDV+ VV+  +D  FPGLY  G L +PWLSDG ++++S+ W S   ILS+++L       
Sbjct: 371 VDVIQVVNCPKDGCFPGLYVTGLLSDPWLSDGHSLMLSTYWRSCRVILSVNLLS------ 430

Query: 441 KFIGEVSRISPSNSNFSWNVLALDGDNVVAVSSSPVDIPQLKYGRLIEKETKNAVWSWLD 500
              GEVSR SPS+S++SWN LALDGD++VAVSSSPV +P++KYG+          W W +
Sbjct: 431 ---GEVSRASPSDSDYSWNALALDGDSIVAVSSSPVSVPEIKYGKKGLDSAGKPSWLWSN 490

Query: 501 VSSPVFKCSEKVVKTLLSALQFSIMKIPVKDVSNCLTKGASKPFEAIFV-SSKSVKGNEL 560
           + SP+ + SEKV+   LS+LQF I+K+P+ DVS  L +GA  P EAI+V SSKS +  + 
Sbjct: 491 IQSPI-RYSEKVMAG-LSSLQFKILKVPISDVSEGLAEGAKNPIEAIYVSSSKSKENGKC 550

Query: 561 NPVIVNLHGGPHSTSISSFSKSSAFLSSLGFNLLVVNYRGSLGFGEDALQSLPGKVGSQD 620
           +P+I  LHGGPHS S  SFS++ A+LSS+G++ L++NYRGSLG+GEDALQSLPGKVGSQD
Sbjct: 551 DPLIAVLHGGPHSVSPCSFSRTMAYLSSIGYSQLIINYRGSLGYGEDALQSLPGKVGSQD 610

Query: 621 VNDVLTALDHIIDKGLANPSKVAVLGGSHGGFLTTHLIGQAPDRFVAAAARNPVCNLALM 680
           V D L A+DH I+ G+A+PS++ VLGGSHGGFLTTHLIGQAPD+FVAAAARNPVCN+A M
Sbjct: 611 VKDCLLAVDHAIEMGIADPSRITVLGGSHGGFLTTHLIGQAPDKFVAAAARNPVCNMASM 670

Query: 681 VGTSDIPDWCYVECYGREWKNYFTEAPSAEHQIELYNKSPILHVSKVKAPTIFLLGAKDL 740
           VG +DIPDWC+ E YG +  +++TEAPSAE     +  SPI H+SKVK PT+FLLG KDL
Sbjct: 671 VGITDIPDWCFFEAYGDQ--SHYTEAPSAEDLSRFHQMSPISHISKVKTPTLFLLGTKDL 730

Query: 741 RVPFSNGLQFARALKEKGVEVKVIMFPDDIHPIDR 773
           RVP SNG Q+ RALKEKGVEVKV++FP+D HP+DR
Sbjct: 731 RVPISNGFQYVRALKEKGVEVKVLVFPNDNHPLDR 743

BLAST of MS007672 vs. ExPASy Swiss-Prot
Match: Q0IXP9 (Acylamino-acid-releasing enzyme 1 OS=Oryza sativa subsp. japonica OX=39947 GN=Os10g0415600 PE=3 SV=1)

HSP 1 Score: 883.2 bits (2281), Expect = 2.1e-255
Identity = 442/758 (58.31%), Postives = 555/758 (73.22%), Query Frame = 0

Query: 22  LPAGVDPETEGEYAEQSKLLQEFTTIPNIDKAWMFKSDS-GDSVATFSISQASLLSNKRR 81
           LP G+D     EYA QSKLLQEF  IP I  AW+F S +   S A  S+ Q  LL+NK+R
Sbjct: 15  LPLGMDVSMVDEYASQSKLLQEFVKIPTIGNAWIFNSKTENTSRAIVSVGQTDLLANKKR 74

Query: 82  KYTLSAHISKGNDDSSVNFKWAPFPVEMIGVSTIVPSPSGSKLLTVRNSENDSPVKLEIW 141
            + L++HISK N  +SV+F+W+PFP+EM GVS ++PSPSG KLL +RNSE+DSP KLE+W
Sbjct: 75  SFLLNSHISK-NSSNSVDFQWSPFPIEMSGVSAVIPSPSGRKLLLIRNSEDDSPTKLEVW 134

Query: 142 SAGQIEKEFHIPQSVHGSIYTDGWFEGISWNSNETYIAYIAEEPSPSKPTFTVSGYQKGC 201
              Q+E E HI QSVHGS+Y D WFEGISWN  ET +AY+AEEP   KP F  SGY+K  
Sbjct: 135 GPCQLENEIHIAQSVHGSLYVDEWFEGISWNQEETLVAYVAEEPPQPKPEFNDSGYKKAG 194

Query: 202 STNKDSTNWKAQGDFEEEWGEAYAGKRQPALFVINIDSGEVRHVKLIEKYLSVGQVVWAP 261
           S+ KD  +WK +GD+EE WGE Y+ KR PALFV+NI SGEVR VK I + LSVGQV+WAP
Sbjct: 195 SSEKDCKSWKGKGDWEETWGETYSKKRIPALFVVNISSGEVRAVKGIPRTLSVGQVIWAP 254

Query: 262 SIGVHQYLVFAGWSSE------PRKLGMKYCYNRPCALYAVRAPDYGSEVDEHKLKDGSK 321
           S      LVF  WSS+      PRKLG+KYC+NRPCALYAV  P +  E D+  L     
Sbjct: 255 SSS--HSLVFVAWSSDNGYQKTPRKLGIKYCFNRPCALYAVPDP-FMEEADKPSLNVSKG 314

Query: 322 EDFPLYNLTQNISSAFLPRFSPDGKFLIFLSANSSVNTGAHSATNSLHRINWPTDEKLCF 381
           E  P   LT  +SSAF PRFSPDGK+L+F+SA S++++G H+ATNS+H+I+WP D KL  
Sbjct: 315 ETAPTTKLTSELSSAFFPRFSPDGKYLVFISAKSAIDSGTHNATNSMHKIDWPADGKL-E 374

Query: 382 NENIVDVVPVVHWAEDDLFPGLYSHGFLINPWLSDGRTVIISSIWHSSEAILSIDILRQF 441
             ++ DVVP+V   +D  FPGLY  G L NPWL+DG+T+I+SSIW S E ILS++++ + 
Sbjct: 375 GLSVADVVPIVMCPQDGCFPGLYCSGILRNPWLTDGQTMILSSIWGSKEVILSVNVVSR- 434

Query: 442 IICFKFIGEVSRISPSNSNFSWNVLALDGDNVVAVSSSPVDIPQLKYGRLIEKETKNAVW 501
                   EVSR+SP +S++SWNVLALD DN++AVSSS + +PQ+ YG  + +  K   W
Sbjct: 435 --------EVSRVSPQDSDYSWNVLALDKDNILAVSSSLITVPQIYYGSEVCQTGKPNQW 494

Query: 502 SWLDVSSPVFKCSEKVVKTLLSALQFSIMKIPVKDVSNCLTKGASKPFEAIFVSSKSVKG 561
            W ++++P    S+K +  +L+  +FSI+KIP+ + SN L  GA  PFEAIFVS K    
Sbjct: 495 EWQEIATPFPSPSDK-ISAILADHKFSILKIPISNSSNKLADGAKLPFEAIFVSWKD--- 554

Query: 562 NELNPVIVNLHGGPHSTSISSFSKSSAFLSSLGFNLLVVNYRGSLGFGEDALQSLPGKVG 621
           +   P IV LHGGPH+   SS+SKS AFL S G+NLLVVNYRGSLGFGE+ALQSLPG +G
Sbjct: 555 SATRPTIVVLHGGPHTVYPSSYSKSLAFLYSQGYNLLVVNYRGSLGFGEEALQSLPGNIG 614

Query: 622 SQDVNDVLTALDHIIDKGLANPSKVAVLGGSHGGFLTTHLIGQAPDRFVAAAARNPVCNL 681
           SQDVNDVLTALD +I KGL + SKVAV+GGSHGGFLTTHLIGQAP  FVAAAARNPVCNL
Sbjct: 615 SQDVNDVLTALDFVIKKGLIDASKVAVVGGSHGGFLTTHLIGQAPGTFVAAAARNPVCNL 674

Query: 682 ALMVGTSDIPDWCYVECYGREWKNYFTEAPSAEHQIELYNKSPILHVSKVKAPTIFLLGA 741
           +LMVGT+DIP+WC+VE YG+E KN F+E PS +   + + KSPI H+SKV  PT+FLLGA
Sbjct: 675 SLMVGTTDIPEWCFVEIYGKEGKNCFSEYPSFDDLCQFHQKSPISHISKVSTPTLFLLGA 734

Query: 742 KDLRVPFSNGLQFARALKEKGVEVKVIMFPDDIHPIDR 773
           +DLRVP SNGLQ+AR LKE GVE K+I+FP+D+H +D+
Sbjct: 735 QDLRVPVSNGLQYARTLKEMGVETKIIVFPEDMHGLDK 754

BLAST of MS007672 vs. ExPASy Swiss-Prot
Match: Q338C0 (Acylamino-acid-releasing enzyme 2 OS=Oryza sativa subsp. japonica OX=39947 GN=Os10g0415800 PE=3 SV=1)

HSP 1 Score: 862.4 bits (2227), Expect = 3.8e-249
Identity = 432/756 (57.14%), Postives = 548/756 (72.49%), Query Frame = 0

Query: 26  VDPETEGEYAEQSKLLQEFTTIPNIDKAWMFKSDSGD-SVATFSISQASLLSNKRRKYTL 85
           +D     EYA QSKLLQEFT  P+ID AW+F++++ D S A +SISQ +LL+N +RKY L
Sbjct: 1   MDALASEEYASQSKLLQEFTNAPSIDGAWVFQTNNEDRSTAMYSISQTNLLANNKRKYIL 60

Query: 86  SAHISKGNDDSSVNFKWAPFPVEMIGVSTIVPSPSGSKLLTVRNSENDSPVKLEIWSAGQ 145
            +HI + N  + ++F+W+PFP++M GVS +VPSPSGSKLL VRN E  SP KLEI     
Sbjct: 61  FSHIMR-NGTNLLDFQWSPFPIQMDGVSAVVPSPSGSKLLVVRNGEKGSPTKLEIVDQSH 120

Query: 146 IEKEFHIPQSVHGSIYTDGWFEGISWNSNETYIAYIAEEPSPSKPTFTVSGYQKGCSTNK 205
           +EKE H+ QSVHG +YTD WF GISWN  ET IAYIAE+    KP F  +GY+K  S+ K
Sbjct: 121 VEKEIHVAQSVHGPLYTDEWFHGISWNQEETLIAYIAEDSPEPKPVFDDTGYRKEGSSEK 180

Query: 206 DSTNWKAQGDFEEEWGEAYAGKRQPALFVINIDSGEVRHVKLIEKYLSVGQVVWAP--SI 265
           D  NWK QGD+EE+WGE Y+ K +P+LFV++I+SGEVR  K I + LSVGQVVWAP  S 
Sbjct: 181 DCNNWKGQGDWEEDWGETYSKKGRPSLFVLDINSGEVRAAKGISRSLSVGQVVWAPPSSC 240

Query: 266 GVHQYLVFAGW------SSEPRKLGMKYCYNRPCALYAVRAPDYGSEVDEHKLKDGSKED 325
           G  +YL+F GW       + PRKLG+KYC NRPC+LY+   P   S+VD     D   E 
Sbjct: 241 GRQKYLIFVGWLEHNGFQNTPRKLGIKYCSNRPCSLYSTLCPFEESDVDNAPASDSKLEP 300

Query: 326 FPL-YNLTQNISSAFLPRFSPDGKFLIFLSANSSVNTGAHSATNSLHRINWPTDEKLCFN 385
             +  NLT +ISSAF PRFS DGK L+FLSAN +V++GAH+AT+SLH+INWP+D K+   
Sbjct: 301 ASVAINLTPSISSAFFPRFSKDGKLLVFLSANRAVDSGAHNATDSLHKINWPSDWKMDQY 360

Query: 386 ENIVDVVPVVHWAEDDLFPGLYSHGFLINPWLSDGRTVIISSIWHSSEAILSIDILRQFI 445
             I DV+P+V   +D  FPGLY    L NPWLSD  T+I++S W S+E ILSID+L    
Sbjct: 361 LEITDVIPIVMCPQDGCFPGLYCSSMLSNPWLSDRCTMILTSAWRSTEVILSIDVLS--- 420

Query: 446 ICFKFIGEVSRISPSNSNFSWNVLALDGDNVVAVSSSPVDIPQLKYGRLIEKETKNAVWS 505
                 G+ +RISP NS +SW+ LA+DG NV+AVSSSP+D PQ+KYG  +  + +   W 
Sbjct: 421 ------GKATRISPENSEYSWSALAVDGHNVLAVSSSPIDPPQIKYGHQVSLKDQTCTWV 480

Query: 506 WLDVSSPVFKCSEKVVKTLLSALQFSIMKIPVKDVSNCLTKGASKPFEAIFVSSKSVKGN 565
           W +V++     +   VK LLS  QFSI+KIPV + S+ L+ G+  PFEAIFVS K    +
Sbjct: 481 WDEVNNNPLMAANNKVKALLSHHQFSILKIPVTNPSDDLSDGSKLPFEAIFVSCKD---S 540

Query: 566 ELNPVIVNLHGGPHSTSISSFSKSSAFLSSLGFNLLVVNYRGSLGFGEDALQSLPGKVGS 625
              P I+ LHGGPHS S+SS+SK+SAFL+SLGFNLL+VNYRG+ GFGE+ALQSLPGKVGS
Sbjct: 541 SHKPTILVLHGGPHSVSVSSYSKTSAFLASLGFNLLIVNYRGTPGFGEEALQSLPGKVGS 600

Query: 626 QDVNDVLTALDHIIDKGLANPSKVAVLGGSHGGFLTTHLIGQAPDRFVAAAARNPVCNLA 685
           QDV D LTALD++I+ GL + SKVAV+G SHGGFLTTHLIGQAPDRF+ AAARNPVCNL+
Sbjct: 601 QDVQDCLTALDYVIEGGLIDASKVAVIGISHGGFLTTHLIGQAPDRFMVAAARNPVCNLS 660

Query: 686 LMVGTSDIPDWCYVECYGREWKNYFTEAPSAEHQIELYNKSPILHVSKVKAPTIFLLGAK 745
           LM+GT+DIPDWCY    G E + + +E+PS +H    Y KSPI H+SKVKAP + LLG  
Sbjct: 661 LMIGTTDIPDWCYAVACGSEGRQHASESPSPDHLRLFYQKSPIAHISKVKAPLLMLLGGA 720

Query: 746 DLRVPFSNGLQFARALKEKGVEVKVIMFPDDIHPID 772
           DLRVP SNGLQ+ARAL+E+G E++++MFPDDIH I+
Sbjct: 721 DLRVPISNGLQYARALRERGGEIRIMMFPDDIHEIN 743

BLAST of MS007672 vs. ExPASy Swiss-Prot
Match: P80227 (Acylamino-acid-releasing enzyme OS=Bos taurus OX=9913 GN=APEH PE=1 SV=2)

HSP 1 Score: 334.0 bits (855), Expect = 4.7e-90
Identity = 221/678 (32.60%), Postives = 340/678 (50.15%), Query Frame = 0

Query: 117 SPSGSKLLTVRNSENDSPVK--LEIWSAGQIEKEFHIPQ-SVHGSIYTDGWFEGISWNSN 176
           SPSG+    +R + +    K  LE+W   +  K F++     HG +Y D  F  +SW+ +
Sbjct: 97  SPSGTMKAVLRKAGSTGEEKQFLEVWEKNRKLKSFNLSALEKHGPVYEDDCFGCLSWSHS 156

Query: 177 ETYIAYIAEEPSPSKPTF------TVSGYQKGCSTNKDSTNWKAQGD---FEEEWGEAYA 236
           ET++ Y+AE+  P   +F       +SG     +  K   +   +GD   F E+WGE   
Sbjct: 157 ETHLLYVAEKKRPKAESFFQTKALDISGSDDEMARPK-KPDQAIKGDQFLFYEDWGENMV 216

Query: 237 GKRQPALFVINIDSGEVRHVKLIEKYLSVGQVVWAPSIGVHQYLVFAGWSSEPRKLGMKY 296
            K  P L V++I+SG +  ++ + + +S GQ  WAP       +VFAGW  EP +LG+++
Sbjct: 217 SKGSPVLCVLDIESGNISVLEGVPESVSPGQAFWAPG---DTGVVFAGWWHEPFRLGIRF 276

Query: 297 CYNRPCALYAVRAPDYGSEVDEHKLKDGSKEDFPLYNLTQNISSAFLPRFSPDGKFLIFL 356
           C NR  ALY V       E+                 L+ +  +   PR SPD   +++L
Sbjct: 277 CTNRRSALYYVDLTGGNCEL-----------------LSDDSLAVTSPRLSPDQCRIVYL 336

Query: 357 SANSSVNTGAHSATNSLHRINWPTDEKLCFNENIVDVVPVVHWAEDDLFPGLYSHGFLIN 416
              S V    H     L   +W T   +     +VDVVP       + F G+Y     + 
Sbjct: 337 QFPSLV---PHQQCGQLCLYDWYTRVTVV----VVDVVP---RQLGENFSGIYCSLLPLG 396

Query: 417 PWLSDGRTVIISSIWHSSEAILSIDILRQFIICFKFIGEVSRISPSNSNFSWNVLALDGD 476
            W +D + V+  +   S + + ++D           +G V+ ++   S  SW +L +D D
Sbjct: 397 CWSADSQRVVFDTAQRSRQDLFAVDTQ---------MGTVTPLTAGGSGGSWKLLTIDRD 456

Query: 477 NVVAVSSSPVDIPQLKYGRL----IEKETKNAVWSWLDVSSPVFKCSEKVVKTLLSALQF 536
            +VA  S+P   P LK G L    +E+E    VW  L+ + P+   S  +       LQ 
Sbjct: 457 LMVAQFSTPNLPPCLKVGFLPPAGMEQE---VVWVSLEEAEPIPDISWSI-----RVLQ- 516

Query: 537 SIMKIPVKDVSNCLTKGASKPFEAIFVSSKSVKGNELNPVIVNLHGGPHSTSISSFSKSS 596
                P  +  +    G    FEAI +   +       P++V  HGGPHS+ ++S+    
Sbjct: 517 -----PPPEQEHAQYVGLD--FEAILIQPSNPPDKTQVPMVVMPHGGPHSSFVTSWMLLP 576

Query: 597 AFLSSLGFNLLVVNYRGSLGFGEDALQSLPGKVGSQDVNDVLTALDHIIDKGLANPSKVA 656
           A L  +GF  L+VNYRGS GFG+D++ SLPG VGSQDV DV  A++ ++ +   +  +VA
Sbjct: 577 AMLCKMGFAALLVNYRGSTGFGQDSILSLPGNVGSQDVKDVQFAVEQVLQEEHFDAGRVA 636

Query: 657 VLGGSHGGFLTTHLIGQAPDRFVAAAARNPVCNLALMVGTSDIPDWCYVECYGREWKNYF 716
           +LGGSHGGFL+ HLIGQ P+ + A   RNPV N+A M+G++DIPDWC VE        Y 
Sbjct: 637 LLGGSHGGFLSCHLIGQYPETYGACVVRNPVINIASMMGSTDIPDWCVVEA------GYL 696

Query: 717 TEA---PSAEHQIELYNKSPILHVSKVKAPTIFLLGAKDLRVPFSNGLQFARALKEKGVE 776
             +   P      E+ NKSPI +  +VK P + +LG +D RVPF  G+++ RALK + V 
Sbjct: 697 YSSDCLPDPNVWSEMLNKSPIKYTPQVKTPVLLMLGQEDRRVPFKQGMEYYRALKARNVP 712

BLAST of MS007672 vs. ExPASy Swiss-Prot
Match: Q8R146 (Acylamino-acid-releasing enzyme OS=Mus musculus OX=10090 GN=Apeh PE=1 SV=3)

HSP 1 Score: 330.9 bits (847), Expect = 4.0e-89
Identity = 215/674 (31.90%), Postives = 338/674 (50.15%), Query Frame = 0

Query: 117 SPSGSKLLTVRNS----ENDSPVKLEIWSAGQIEKEFHIPQ-SVHGSIYTDGWFEGISWN 176
           SPSG+    +R +      +    LE+W   +  K F++     HG +Y D  F  +SW+
Sbjct: 97  SPSGTMKAVLRKAGGAVSGEEKQFLEVWEKNRKLKSFNLSALEKHGPVYEDDCFGCLSWS 156

Query: 177 SNETYIAYIAEEPSPSKPTFTVSGYQKGCSTNKDSTNWK-----AQGD---FEEEWGEAY 236
            +ET++ Y+AE+  P   +F  +      +++++    K      +GD   F E+WGE  
Sbjct: 157 HSETHLLYVAEKKRPKAESFFQTKALDVSASDEEMARPKKPDQAIKGDQFVFYEDWGETM 216

Query: 237 AGKRQPALFVINIDSGEVRHVKLIEKYLSVGQVVWAPSIGVHQYLVFAGWSSEPRKLGMK 296
             K  P L V++I+SG +  ++ + + +S GQ  WAP       +VF GW  EP +LG++
Sbjct: 217 VSKSIPVLCVLDIESGNISVLEGVPENVSPGQAFWAPG---DTGVVFVGWWHEPFRLGIR 276

Query: 297 YCYNRPCALYAVRAPDYGSEVDEHKLKDGSKEDFPLYNLTQNISSAFLPRFSPDGKFLIF 356
           YC NR  ALY V             L  G  E      L+    +   PR SPD   +++
Sbjct: 277 YCTNRRSALYYV------------DLSGGKCE-----LLSDESLAVCSPRLSPDQCRVVY 336

Query: 357 LSANSSVNTGAHSATNSLHRINWPTDEKLCFNENIVDVVPVVHWAEDDLFPGLYSHGFLI 416
           L   S      H   + L   +W T         +VD+VP       + F G+Y     +
Sbjct: 337 LQYPS---LAPHHQCSQLFLYDWYTK----VTSLVVDIVP---RQLGESFSGIYCSLLPL 396

Query: 417 NPWLSDGRTVIISSIWHSSEAILSIDILRQFIICFKFIGEVSRISPSNSNFSWNVLALDG 476
             W +D + V+  S+  S + + ++D            G V+ ++   S  SW +L +D 
Sbjct: 397 GCWSADSQRVVFDSVQRSRQDLFAVDTQ---------TGSVTSLTAGGSAGSWKLLTIDR 456

Query: 477 DNVVAVSSSPVDIPQLKYGRLIEKETKNAV-WSWLDVSSPVFKCSEKVVKTLLSALQFSI 536
           D +VA  S+P   P LK G L     + +V W  L+ + P+              + + I
Sbjct: 457 DLMVAQFSTPNLPPSLKVGFLPPAGKEQSVSWVSLEEAEPI------------PDIHWGI 516

Query: 537 MKI-PVKDVSNCLTKGASKPFEAIFVSSKSVKGNELNPVIVNLHGGPHSTSISSFSKSSA 596
             + P  D  N   + A   FEAI +   +       P++V  HGGPHS+ ++++    A
Sbjct: 517 RVLHPPPDQEN--VQYADLDFEAILLQPSNSPDKSQVPMVVMPHGGPHSSFVTAWMLFPA 576

Query: 597 FLSSLGFNLLVVNYRGSLGFGEDALQSLPGKVGSQDVNDVLTALDHIIDKGLANPSKVAV 656
            L  +GF +L+VNYRGS GFG+D++ SLPG VG QDV DV  A+  ++ +   +  +VA+
Sbjct: 577 MLCKMGFAVLLVNYRGSTGFGQDSILSLPGNVGHQDVKDVQFAVQQVLQEEHFDARRVAL 636

Query: 657 LGGSHGGFLTTHLIGQAPDRFVAAAARNPVCNLALMVGTSDIPDWCYVECYGREWKNYFT 716
           +GGSHGGFL+ HLIGQ P+ + A  ARNPV N+  M+GT+DIPDWC VE  G  + N + 
Sbjct: 637 MGGSHGGFLSCHLIGQYPETYSACIARNPVINIVSMMGTTDIPDWCMVET-GFPYSNDY- 696

Query: 717 EAPSAEHQIELYNKSPILHVSKVKAPTIFLLGAKDLRVPFSNGLQFARALKEKGVEVKVI 776
             P      E+ +KSPI ++ +VK P + +LG +D RVPF  GL++  ALK + V V+++
Sbjct: 697 -LPDLNVLEEMLDKSPIKYIPQVKTPVLLMLGQEDRRVPFKQGLEYYHALKARNVPVRLL 714

BLAST of MS007672 vs. ExPASy TrEMBL
Match: A0A6J1CDB8 (acylamino-acid-releasing enzyme isoform X2 OS=Momordica charantia OX=3673 GN=LOC111009690 PE=4 SV=1)

HSP 1 Score: 1523.1 bits (3942), Expect = 0.0e+00
Identity = 757/772 (98.06%), Postives = 758/772 (98.19%), Query Frame = 0

Query: 1   SRKLSTSFAMDGSKISAVVDELPAGVDPETEGEYAEQSKLLQEFTTIPNIDKAWMFKSDS 60
           +RKLSTSFAMDGSKISAVVDELPAGVDPETEGEYAEQSKLLQEFTTIPNIDKAWMFKSDS
Sbjct: 42  TRKLSTSFAMDGSKISAVVDELPAGVDPETEGEYAEQSKLLQEFTTIPNIDKAWMFKSDS 101

Query: 61  GDSVATFSISQASLLSNKRRKYTLSAHISKGNDDSSVNFKWAPFPVEMIGVSTIVPSPSG 120
           GDSVATFSISQASLLSNKRRKYTLSAHISKGN DSSVNFKWAPFPVEMIGVSTIVPSPSG
Sbjct: 102 GDSVATFSISQASLLSNKRRKYTLSAHISKGNGDSSVNFKWAPFPVEMIGVSTIVPSPSG 161

Query: 121 SKLLTVRNSENDSPVKLEIWSAGQIEKEFHIPQSVHGSIYTDGWFEGISWNSNETYIAYI 180
           SKLLTVRNSENDSPVKLEIWSAGQIEKEFHIPQSVHGSIYTDGWFEGISWNSNETYIAYI
Sbjct: 162 SKLLTVRNSENDSPVKLEIWSAGQIEKEFHIPQSVHGSIYTDGWFEGISWNSNETYIAYI 221

Query: 181 AEEPSPSKPTFTVSGYQKGCSTNKDSTNWKAQGDFEEEWGEAYAGKRQPALFVINIDSGE 240
           AEEPSPSKPTFTVSGYQKGCSTNKDSTNWKAQGDFEEEWGEAYAGKRQPALFVINIDSGE
Sbjct: 222 AEEPSPSKPTFTVSGYQKGCSTNKDSTNWKAQGDFEEEWGEAYAGKRQPALFVINIDSGE 281

Query: 241 VRHVKLIEKYLSVGQVVWAPSIGVHQYLVFAGWSSEPRKLGMKYCYNRPCALYAVRAPDY 300
           VRHVKLIEKYLSVGQVVWAPSIGVHQYLVFAGWSSEPRKLGMKYCYNRPCALYAVRAPDY
Sbjct: 282 VRHVKLIEKYLSVGQVVWAPSIGVHQYLVFAGWSSEPRKLGMKYCYNRPCALYAVRAPDY 341

Query: 301 GSEVDEHKLKDGSKEDFPLYNLTQNISSAFLPRFSPDGKFLIFLSANSSVNTGAHSATNS 360
           GSEVDEHKLKDGSKEDFPLYNLTQNISSAFLPRFSPDGKFLIFLSANSSVNTGAHSATNS
Sbjct: 342 GSEVDEHKLKDGSKEDFPLYNLTQNISSAFLPRFSPDGKFLIFLSANSSVNTGAHSATNS 401

Query: 361 LHRINWPTDEKLCFNENIVDVVPVVHWAEDDLFPGLYSHGFLINPWLSDGRTVIISSIWH 420
           LHRINWPTDEKLCFNENIVDVVPVVHWAEDDLFPGLYSHGFLINPWLSDGRTVIISSIWH
Sbjct: 402 LHRINWPTDEKLCFNENIVDVVPVVHWAEDDLFPGLYSHGFLINPWLSDGRTVIISSIWH 461

Query: 421 SSEAILSIDILRQFIICFKFIGEVSRISPSNSNFSWNVLALDGDNVVAVSSSPVDIPQLK 480
           SSEAILSIDIL          GEVSRISPSNSNFSWNVLALDGDNVVAVSSSPVDIPQLK
Sbjct: 462 SSEAILSIDILS---------GEVSRISPSNSNFSWNVLALDGDNVVAVSSSPVDIPQLK 521

Query: 481 YGRLIEKETKNAVWSWLDVSSPVFKCSEKVVKTLLSALQFSIMKIPVKDVSNCLTKGASK 540
           YGRLIEKETKNAVWSWLDVSSPVFKCSEK VKTLLSALQFSIMKIPVKDVSNCLTKGASK
Sbjct: 522 YGRLIEKETKNAVWSWLDVSSPVFKCSEK-VKTLLSALQFSIMKIPVKDVSNCLTKGASK 581

Query: 541 PFEAIFVSSKSVKGNELNPVIVNLHGGPHSTSISSFSKSSAFLSSLGFNLLVVNYRGSLG 600
           PFEAIFVSSKSVKGNELNPVIVNLHGGPHSTSISSFSKSSAFLSSLGFNLLVVNYRGSLG
Sbjct: 582 PFEAIFVSSKSVKGNELNPVIVNLHGGPHSTSISSFSKSSAFLSSLGFNLLVVNYRGSLG 641

Query: 601 FGEDALQSLPGKVGSQDVNDVLTALDHIIDKGLANPSKVAVLGGSHGGFLTTHLIGQAPD 660
           FGEDALQSLPGKVGSQDVNDVL ALDHIIDKGLANPSKVAVLGGSHGGFLTTHLIGQAPD
Sbjct: 642 FGEDALQSLPGKVGSQDVNDVLAALDHIIDKGLANPSKVAVLGGSHGGFLTTHLIGQAPD 701

Query: 661 RFVAAAARNPVCNLALMVGTSDIPDWCYVECYGREWKNYFTEAPSAEHQIELYNKSPILH 720
           RFVAAAARNPVCNLALMVGTSDIPDWCYVECYGRE KNYFTEAPSAEHQIELYNKSPILH
Sbjct: 702 RFVAAAARNPVCNLALMVGTSDIPDWCYVECYGREGKNYFTEAPSAEHQIELYNKSPILH 761

Query: 721 VSKVKAPTIFLLGAKDLRVPFSNGLQFARALKEKGVEVKVIMFPDDIHPIDR 773
           VSKVKAPTIFLLGAKDLRVPFSNGLQFARALKEKGVEVKVIMFPDDIHPIDR
Sbjct: 762 VSKVKAPTIFLLGAKDLRVPFSNGLQFARALKEKGVEVKVIMFPDDIHPIDR 803

BLAST of MS007672 vs. ExPASy TrEMBL
Match: A0A6J1CBF8 (acylamino-acid-releasing enzyme isoform X1 OS=Momordica charantia OX=3673 GN=LOC111009690 PE=4 SV=1)

HSP 1 Score: 1516.9 bits (3926), Expect = 0.0e+00
Identity = 757/777 (97.43%), Postives = 758/777 (97.55%), Query Frame = 0

Query: 1   SRKLSTSFAMDGSKISAVVDELPAGVDPETEGEYAEQSKLLQEFTTIPNIDKAWMFKSDS 60
           +RKLSTSFAMDGSKISAVVDELPAGVDPETEGEYAEQSKLLQEFTTIPNIDKAWMFKSDS
Sbjct: 42  TRKLSTSFAMDGSKISAVVDELPAGVDPETEGEYAEQSKLLQEFTTIPNIDKAWMFKSDS 101

Query: 61  GDSVATFSISQASLLSNKRRKYTLSAHISKGNDDSSVNFKWAPFPVEMIGVSTIVPSPSG 120
           GDSVATFSISQASLLSNKRRKYTLSAHISKGN DSSVNFKWAPFPVEMIGVSTIVPSPSG
Sbjct: 102 GDSVATFSISQASLLSNKRRKYTLSAHISKGNGDSSVNFKWAPFPVEMIGVSTIVPSPSG 161

Query: 121 SKLLTVRNSENDSPVKLEIWSAGQIEKEFHIPQSVHGSIYTDGWFEGISWNSNETYIAYI 180
           SKLLTVRNSENDSPVKLEIWSAGQIEKEFHIPQSVHGSIYTDGWFEGISWNSNETYIAYI
Sbjct: 162 SKLLTVRNSENDSPVKLEIWSAGQIEKEFHIPQSVHGSIYTDGWFEGISWNSNETYIAYI 221

Query: 181 AEEPSPSKPTFTVSGYQKGCSTNKDSTNWKAQGDFEEEWGEAYAGKRQPALFVINIDSGE 240
           AEEPSPSKPTFTVSGYQKGCSTNKDSTNWKAQGDFEEEWGEAYAGKRQPALFVINIDSGE
Sbjct: 222 AEEPSPSKPTFTVSGYQKGCSTNKDSTNWKAQGDFEEEWGEAYAGKRQPALFVINIDSGE 281

Query: 241 VRHVKLIEKYLSVGQVVWAPSIGVHQYLVFAGWSSEPRKLGMKYCYNRPCALYAVRAPDY 300
           VRHVKLIEKYLSVGQVVWAPSIGVHQYLVFAGWSSEPRKLGMKYCYNRPCALYAVRAPDY
Sbjct: 282 VRHVKLIEKYLSVGQVVWAPSIGVHQYLVFAGWSSEPRKLGMKYCYNRPCALYAVRAPDY 341

Query: 301 GSEVDEHKLKDGSKEDFPLYNLTQNISSAFLPRFSPDGKFLIFLSANSSVNTGAHSATNS 360
           GSEVDEHKLKDGSKEDFPLYNLTQNISSAFLPRFSPDGKFLIFLSANSSVNTGAHSATNS
Sbjct: 342 GSEVDEHKLKDGSKEDFPLYNLTQNISSAFLPRFSPDGKFLIFLSANSSVNTGAHSATNS 401

Query: 361 LHRINWPTDEKLCFNENIVDVVPVVHWAEDDLFPGLYSHGFLINPWLSDGRTVIISSIWH 420
           LHRINWPTDEKLCFNENIVDVVPVVHWAEDDLFPGLYSHGFLINPWLSDGRTVIISSIWH
Sbjct: 402 LHRINWPTDEKLCFNENIVDVVPVVHWAEDDLFPGLYSHGFLINPWLSDGRTVIISSIWH 461

Query: 421 SSEAILSIDILRQFIICFKFIGEVSRISPSNSNFSWNVLALDGDNVVAVSSSPVDIPQLK 480
           SSEAILSIDIL          GEVSRISPSNSNFSWNVLALDGDNVVAVSSSPVDIPQLK
Sbjct: 462 SSEAILSIDILS---------GEVSRISPSNSNFSWNVLALDGDNVVAVSSSPVDIPQLK 521

Query: 481 YGRLIEKETKNAVWSWLDVSSPVFKCSEKVVKTLLSALQFSIMKIPVKDVSNCLTK---- 540
           YGRLIEKETKNAVWSWLDVSSPVFKCSEK VKTLLSALQFSIMKIPVKDVSNCLTK    
Sbjct: 522 YGRLIEKETKNAVWSWLDVSSPVFKCSEK-VKTLLSALQFSIMKIPVKDVSNCLTKVTFF 581

Query: 541 -GASKPFEAIFVSSKSVKGNELNPVIVNLHGGPHSTSISSFSKSSAFLSSLGFNLLVVNY 600
            GASKPFEAIFVSSKSVKGNELNPVIVNLHGGPHSTSISSFSKSSAFLSSLGFNLLVVNY
Sbjct: 582 SGASKPFEAIFVSSKSVKGNELNPVIVNLHGGPHSTSISSFSKSSAFLSSLGFNLLVVNY 641

Query: 601 RGSLGFGEDALQSLPGKVGSQDVNDVLTALDHIIDKGLANPSKVAVLGGSHGGFLTTHLI 660
           RGSLGFGEDALQSLPGKVGSQDVNDVL ALDHIIDKGLANPSKVAVLGGSHGGFLTTHLI
Sbjct: 642 RGSLGFGEDALQSLPGKVGSQDVNDVLAALDHIIDKGLANPSKVAVLGGSHGGFLTTHLI 701

Query: 661 GQAPDRFVAAAARNPVCNLALMVGTSDIPDWCYVECYGREWKNYFTEAPSAEHQIELYNK 720
           GQAPDRFVAAAARNPVCNLALMVGTSDIPDWCYVECYGRE KNYFTEAPSAEHQIELYNK
Sbjct: 702 GQAPDRFVAAAARNPVCNLALMVGTSDIPDWCYVECYGREGKNYFTEAPSAEHQIELYNK 761

Query: 721 SPILHVSKVKAPTIFLLGAKDLRVPFSNGLQFARALKEKGVEVKVIMFPDDIHPIDR 773
           SPILHVSKVKAPTIFLLGAKDLRVPFSNGLQFARALKEKGVEVKVIMFPDDIHPIDR
Sbjct: 762 SPILHVSKVKAPTIFLLGAKDLRVPFSNGLQFARALKEKGVEVKVIMFPDDIHPIDR 808

BLAST of MS007672 vs. ExPASy TrEMBL
Match: A0A6J1CAF4 (acylamino-acid-releasing enzyme isoform X3 OS=Momordica charantia OX=3673 GN=LOC111009690 PE=4 SV=1)

HSP 1 Score: 1502.6 bits (3889), Expect = 0.0e+00
Identity = 749/768 (97.53%), Postives = 749/768 (97.53%), Query Frame = 0

Query: 10  MDGSKISAVVDELPAGVDPETEGEYAEQSKLLQEFTTIPNIDKAWMFKSDSGDSVATFSI 69
           MDGSKISAVVDELPAGVDPETEGEYAEQSKLLQEFTTIPNIDKAWMFKSDSGDSVATFSI
Sbjct: 1   MDGSKISAVVDELPAGVDPETEGEYAEQSKLLQEFTTIPNIDKAWMFKSDSGDSVATFSI 60

Query: 70  SQASLLSNKRRKYTLSAHISKGNDDSSVNFKWAPFPVEMIGVSTIVPSPSGSKLLTVRNS 129
           SQASLLSNKRRKYTLSAHISKGN DSSVNFKWAPFPVEMIGVSTIVPSPSGSKLLTVRNS
Sbjct: 61  SQASLLSNKRRKYTLSAHISKGNGDSSVNFKWAPFPVEMIGVSTIVPSPSGSKLLTVRNS 120

Query: 130 ENDSPVKLEIWSAGQIEKEFHIPQSVHGSIYTDGWFEGISWNSNETYIAYIAEEPSPSKP 189
           ENDSPVKLEIWSAGQIEKEFHIPQSVHGSIYTDGWFEGISWNSNETYIAYIAEEPSPSKP
Sbjct: 121 ENDSPVKLEIWSAGQIEKEFHIPQSVHGSIYTDGWFEGISWNSNETYIAYIAEEPSPSKP 180

Query: 190 TFTVSGYQKGCSTNKDSTNWKAQGDFEEEWGEAYAGKRQPALFVINIDSGEVRHVKLIEK 249
           TFTVSGYQKGCSTNKDSTNWKAQGDFEEEWGEAYAGKRQPALFVINIDSGEVRHVKLIEK
Sbjct: 181 TFTVSGYQKGCSTNKDSTNWKAQGDFEEEWGEAYAGKRQPALFVINIDSGEVRHVKLIEK 240

Query: 250 YLSVGQVVWAPSIGVHQYLVFAGWSSEPRKLGMKYCYNRPCALYAVRAPDYGSEVDEHKL 309
           YLSVGQVVWAPSIGVHQYLVFAGWSSEPRKLGMKYCYNRPCALYAVRAPDYGSEVDEHKL
Sbjct: 241 YLSVGQVVWAPSIGVHQYLVFAGWSSEPRKLGMKYCYNRPCALYAVRAPDYGSEVDEHKL 300

Query: 310 KDGSKEDFPLYNLTQNISSAFLPRFSPDGKFLIFLSANSSVNTGAHSATNSLHRINWPTD 369
           KDGSKEDFPLYNLTQNISSAFLPRFSPDGKFLIFLSANSSVNTGAHSATNSLHRINWPTD
Sbjct: 301 KDGSKEDFPLYNLTQNISSAFLPRFSPDGKFLIFLSANSSVNTGAHSATNSLHRINWPTD 360

Query: 370 EKLCFNENIVDVVPVVHWAEDDLFPGLYSHGFLINPWLSDGRTVIISSIWHSSEAILSID 429
           EKLCFNENIVDVVPVVHWAEDDLFPGLYSHGFLINPWLSDGRTVIISSIWHSSEAILSID
Sbjct: 361 EKLCFNENIVDVVPVVHWAEDDLFPGLYSHGFLINPWLSDGRTVIISSIWHSSEAILSID 420

Query: 430 ILRQFIICFKFIGEVSRISPSNSNFSWNVLALDGDNVVAVSSSPVDIPQLKYGRLIEKET 489
           IL          GEVSRISPSNSNFSWNVLALDGDNVVAVSSSPVDIPQLKYGRLIEKET
Sbjct: 421 ILS---------GEVSRISPSNSNFSWNVLALDGDNVVAVSSSPVDIPQLKYGRLIEKET 480

Query: 490 KNAVWSWLDVSSPVFKCSEKVVKTLLSALQFSIMKIPVKDVSNCLTK-----GASKPFEA 549
           KNAVWSWLDVSSPVFKCSEK VKTLLSALQFSIMKIPVKDVSNCLTK     GASKPFEA
Sbjct: 481 KNAVWSWLDVSSPVFKCSEK-VKTLLSALQFSIMKIPVKDVSNCLTKVTFFSGASKPFEA 540

Query: 550 IFVSSKSVKGNELNPVIVNLHGGPHSTSISSFSKSSAFLSSLGFNLLVVNYRGSLGFGED 609
           IFVSSKSVKGNELNPVIVNLHGGPHSTSISSFSKSSAFLSSLGFNLLVVNYRGSLGFGED
Sbjct: 541 IFVSSKSVKGNELNPVIVNLHGGPHSTSISSFSKSSAFLSSLGFNLLVVNYRGSLGFGED 600

Query: 610 ALQSLPGKVGSQDVNDVLTALDHIIDKGLANPSKVAVLGGSHGGFLTTHLIGQAPDRFVA 669
           ALQSLPGKVGSQDVNDVL ALDHIIDKGLANPSKVAVLGGSHGGFLTTHLIGQAPDRFVA
Sbjct: 601 ALQSLPGKVGSQDVNDVLAALDHIIDKGLANPSKVAVLGGSHGGFLTTHLIGQAPDRFVA 660

Query: 670 AAARNPVCNLALMVGTSDIPDWCYVECYGREWKNYFTEAPSAEHQIELYNKSPILHVSKV 729
           AAARNPVCNLALMVGTSDIPDWCYVECYGRE KNYFTEAPSAEHQIELYNKSPILHVSKV
Sbjct: 661 AAARNPVCNLALMVGTSDIPDWCYVECYGREGKNYFTEAPSAEHQIELYNKSPILHVSKV 720

Query: 730 KAPTIFLLGAKDLRVPFSNGLQFARALKEKGVEVKVIMFPDDIHPIDR 773
           KAPTIFLLGAKDLRVPFSNGLQFARALKEKGVEVKVIMFPDDIHPIDR
Sbjct: 721 KAPTIFLLGAKDLRVPFSNGLQFARALKEKGVEVKVIMFPDDIHPIDR 758

BLAST of MS007672 vs. ExPASy TrEMBL
Match: A0A5A7UVB5 (Acylamino-acid-releasing enzyme OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold280G003600 PE=4 SV=1)

HSP 1 Score: 1364.7 bits (3531), Expect = 0.0e+00
Identity = 668/782 (85.42%), Postives = 715/782 (91.43%), Query Frame = 0

Query: 1   SRKLSTSFAMDGSKISAVVDELPAGVDPETEGEYAEQSKLLQEFTTIPNIDKAWMFKSDS 60
           SR+LS +  MDGS IS V DE P+G+DP TE EYAEQSKLLQEFT IPNID+AW FKSDS
Sbjct: 42  SRQLSAAMVMDGSTISKVADEFPSGIDPTTEEEYAEQSKLLQEFTKIPNIDRAWTFKSDS 101

Query: 61  GDSVATFSISQASLLSNKRRKYTLSAHISKGNDDSSVNFKWAPFPVEMIGVSTIVPSPSG 120
           GD +ATFSISQASLL+NKRRKYTLSAHISKGND +SVNF W PFP+EMIGVSTIVPSPSG
Sbjct: 102 GDPMATFSISQASLLANKRRKYTLSAHISKGNDGNSVNFTWTPFPIEMIGVSTIVPSPSG 161

Query: 121 SKLLTVRNSENDSPVKLEIWSAGQIEKEFHIPQSVHGSIYTDGWFEGISWNSNETYIAYI 180
           SK LTVRN ENDSPV+LEIWSAGQIEKEFHIPQS+HGSIYTDGWFEGISWNSNETYIAY+
Sbjct: 162 SKFLTVRNPENDSPVQLEIWSAGQIEKEFHIPQSIHGSIYTDGWFEGISWNSNETYIAYV 221

Query: 181 AEEPSPSKPTFTVSGYQKGCSTNKDSTNWKAQGDFEEEWGEAYAGKRQPALFVINIDSGE 240
           AEEPSPSKPTFT SGYQKGCS NKDSTNWK QGDF+E+WGEAYAGKRQPALFVINIDSGE
Sbjct: 222 AEEPSPSKPTFTFSGYQKGCSANKDSTNWKGQGDFKEDWGEAYAGKRQPALFVINIDSGE 281

Query: 241 VRHVKLIEKYLSVGQVVWAPSIGVHQYLVFAGWSSEPRKLGMKYCYNRPCALYAVRAPDY 300
           VRHVK++EK LSVGQV+WAPSIG  QYLVF GWSSEPRKLGM YCYNRPCALYAV+APDY
Sbjct: 282 VRHVKIVEKSLSVGQVIWAPSIGEDQYLVFVGWSSEPRKLGMIYCYNRPCALYAVKAPDY 341

Query: 301 GSEVDEHKLKDGSKEDFPLYNLTQNISSAFLPRFSPDGKFLIFLSANSSVNTGAHSATNS 360
           GSE++E KLKD  KEDFPLYNLTQ+ISSAF PRFSPDGKFL+FLSA+SSVN+GAHSATNS
Sbjct: 342 GSEINERKLKDEPKEDFPLYNLTQSISSAFFPRFSPDGKFLVFLSAHSSVNSGAHSATNS 401

Query: 361 LHRINWPTDEKLCFNENIVDVVPVVHWAEDDLFPGLYSHGFLINPWLSDGRTVIISSIWH 420
           LHRINWP D K  F+ENIVDVVPVVH+AE++LFPGLYSHGFL NPWLSDG TVI +SIW 
Sbjct: 402 LHRINWPRDGKPSFSENIVDVVPVVHYAENELFPGLYSHGFLSNPWLSDGHTVITTSIWR 461

Query: 421 SSEAILSIDILRQFIICFKFI----------GEVSRISPSNSNFSWNVLALDGDNVVAVS 480
           S +AILSIDILRQF   FK +          GEVS ISP+NSNFSW VLALDGDNVVAVS
Sbjct: 462 SKDAILSIDILRQFEYFFKKLLFCAKLPFNSGEVSCISPANSNFSWKVLALDGDNVVAVS 521

Query: 481 SSPVDIPQLKYGRLIEKETKNAVWSWLDVSSPVFKCSEKVVKTLLSALQFSIMKIPVKDV 540
           SSPVDIPQLKYG L EKE+KNA WSWLDVSSPVFKCSEK VK LLS+LQFS MKIPVKDV
Sbjct: 522 SSPVDIPQLKYGCLTEKESKNAAWSWLDVSSPVFKCSEK-VKNLLSSLQFSTMKIPVKDV 581

Query: 541 SNCLTKGASKPFEAIFVSSKSVKGNELNPVIVNLHGGPHSTSISSFSKSSAFLSSLGFNL 600
           SNCLTKGAS+PFEAIFVSSKSVKGNELNP+IVNLHGGPHSTSISSFSKS AFLSS+GFNL
Sbjct: 582 SNCLTKGASEPFEAIFVSSKSVKGNELNPLIVNLHGGPHSTSISSFSKSLAFLSSIGFNL 641

Query: 601 LVVNYRGSLGFGEDALQSLPGKVGSQDVNDVLTALDHIIDKGLANPSKVAVLGGSHGGFL 660
           LVVNYRGSLGFGE+ALQSLPGK+G+QDVNDVLTALDHIIDKGLA+ SKVAVLGGSHGGFL
Sbjct: 642 LVVNYRGSLGFGEEALQSLPGKIGTQDVNDVLTALDHIIDKGLASSSKVAVLGGSHGGFL 701

Query: 661 TTHLIGQAPDRFVAAAARNPVCNLALMVGTSDIPDWCYVECYGREWKNYFTEAPSAEHQI 720
           TTHLIGQAPDRFVAAAARNPVCNLALMVGTSDIPDWCYVECYG+E KN++TEAPSAEH  
Sbjct: 702 TTHLIGQAPDRFVAAAARNPVCNLALMVGTSDIPDWCYVECYGKEGKNHYTEAPSAEHLT 761

Query: 721 ELYNKSPILHVSKVKAPTIFLLGAKDLRVPFSNGLQFARALKEKGVEVKVIMFPDDIHPI 773
            LYNKSPILHVSKVK PTIFLLGAKDLRVPFSNGLQ+ARAL+EKGVEVKVIMFPDD+HPI
Sbjct: 762 HLYNKSPILHVSKVKTPTIFLLGAKDLRVPFSNGLQYARALREKGVEVKVIMFPDDVHPI 821

BLAST of MS007672 vs. ExPASy TrEMBL
Match: A0A5D3E4H1 (Acylamino-acid-releasing enzyme OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold216G001630 PE=4 SV=1)

HSP 1 Score: 1357.8 bits (3513), Expect = 0.0e+00
Identity = 663/772 (85.88%), Postives = 709/772 (91.84%), Query Frame = 0

Query: 1   SRKLSTSFAMDGSKISAVVDELPAGVDPETEGEYAEQSKLLQEFTTIPNIDKAWMFKSDS 60
           SR+LS +  MDGS IS V DE P+G+DP TE EYAEQSKLLQEFT IPNID+AW FKSDS
Sbjct: 42  SRQLSAAMVMDGSTISKVADEFPSGIDPTTEEEYAEQSKLLQEFTKIPNIDRAWTFKSDS 101

Query: 61  GDSVATFSISQASLLSNKRRKYTLSAHISKGNDDSSVNFKWAPFPVEMIGVSTIVPSPSG 120
           GD +ATFSISQASLL+NKRRKYTLSAHISKGND +SVNF W PFP+EMIGVSTIVPSPSG
Sbjct: 102 GDPMATFSISQASLLANKRRKYTLSAHISKGNDGNSVNFTWTPFPIEMIGVSTIVPSPSG 161

Query: 121 SKLLTVRNSENDSPVKLEIWSAGQIEKEFHIPQSVHGSIYTDGWFEGISWNSNETYIAYI 180
           SK LTVRN ENDSPV+LEIWSAGQIEKEFHIPQS+HGSIYTDGWFEGISWNSNETYIAY+
Sbjct: 162 SKFLTVRNPENDSPVQLEIWSAGQIEKEFHIPQSIHGSIYTDGWFEGISWNSNETYIAYV 221

Query: 181 AEEPSPSKPTFTVSGYQKGCSTNKDSTNWKAQGDFEEEWGEAYAGKRQPALFVINIDSGE 240
           AEEPSPSKPTFT SGYQKGCS NKDSTNWK QGDF+E+WGEAYAGKRQPALFVINIDSGE
Sbjct: 222 AEEPSPSKPTFTFSGYQKGCSANKDSTNWKGQGDFKEDWGEAYAGKRQPALFVINIDSGE 281

Query: 241 VRHVKLIEKYLSVGQVVWAPSIGVHQYLVFAGWSSEPRKLGMKYCYNRPCALYAVRAPDY 300
           VRHVK++EK LSVGQV+WAPSIG  QYLVF GWSSEPRKLGM YCYNRPCALYAV+APDY
Sbjct: 282 VRHVKIVEKSLSVGQVIWAPSIGEDQYLVFVGWSSEPRKLGMIYCYNRPCALYAVKAPDY 341

Query: 301 GSEVDEHKLKDGSKEDFPLYNLTQNISSAFLPRFSPDGKFLIFLSANSSVNTGAHSATNS 360
           GSE++E KLKD  KEDFPLYNLTQ+ISSAF PRFSPDGKFL+FLSA+SSVN+GAHSATNS
Sbjct: 342 GSEINERKLKDEPKEDFPLYNLTQSISSAFFPRFSPDGKFLVFLSAHSSVNSGAHSATNS 401

Query: 361 LHRINWPTDEKLCFNENIVDVVPVVHWAEDDLFPGLYSHGFLINPWLSDGRTVIISSIWH 420
           LHRINWP D K  F+ENIVDVVPVVH+AE++LFPGLYSHGFL NPWLSDG TVI +SIW 
Sbjct: 402 LHRINWPRDGKPSFSENIVDVVPVVHYAENELFPGLYSHGFLSNPWLSDGHTVITTSIWR 461

Query: 421 SSEAILSIDILRQFIICFKFIGEVSRISPSNSNFSWNVLALDGDNVVAVSSSPVDIPQLK 480
           S +AILSIDIL          GEVS ISP+NSNFSW VLALDGDNVVAVSSSPVDIPQLK
Sbjct: 462 SKDAILSIDILS---------GEVSCISPANSNFSWKVLALDGDNVVAVSSSPVDIPQLK 521

Query: 481 YGRLIEKETKNAVWSWLDVSSPVFKCSEKVVKTLLSALQFSIMKIPVKDVSNCLTKGASK 540
           YG L EKE+KNA WSWLDVSSPVFKCSEK VK LLS+LQFS MKIPVKDVSNCLTKGAS+
Sbjct: 522 YGCLTEKESKNAAWSWLDVSSPVFKCSEK-VKNLLSSLQFSTMKIPVKDVSNCLTKGASE 581

Query: 541 PFEAIFVSSKSVKGNELNPVIVNLHGGPHSTSISSFSKSSAFLSSLGFNLLVVNYRGSLG 600
           PFEAIFVSSKSVKGNELNP+IVNLHGGPHSTSISSFSKS AFLSS+GFNLLVVNYRGSLG
Sbjct: 582 PFEAIFVSSKSVKGNELNPLIVNLHGGPHSTSISSFSKSLAFLSSIGFNLLVVNYRGSLG 641

Query: 601 FGEDALQSLPGKVGSQDVNDVLTALDHIIDKGLANPSKVAVLGGSHGGFLTTHLIGQAPD 660
           FGE+ALQSLPGK+G+QDVNDVLTALDHIIDKGLA+ SKVAVLGGSHGGFLTTHLIGQAPD
Sbjct: 642 FGEEALQSLPGKIGTQDVNDVLTALDHIIDKGLASSSKVAVLGGSHGGFLTTHLIGQAPD 701

Query: 661 RFVAAAARNPVCNLALMVGTSDIPDWCYVECYGREWKNYFTEAPSAEHQIELYNKSPILH 720
           RFVAAAARNPVCNLALMVGTSDIPDWCYVECYG+E KN++TEAPSAEH   LYNKSPILH
Sbjct: 702 RFVAAAARNPVCNLALMVGTSDIPDWCYVECYGKEGKNHYTEAPSAEHLTHLYNKSPILH 761

Query: 721 VSKVKAPTIFLLGAKDLRVPFSNGLQFARALKEKGVEVKVIMFPDDIHPIDR 773
           VSKVK PTIFLLGAKDLRVPFSNGLQ+ARAL+EKGVEVKVIMFPDD+HPIDR
Sbjct: 762 VSKVKTPTIFLLGAKDLRVPFSNGLQYARALREKGVEVKVIMFPDDVHPIDR 803

BLAST of MS007672 vs. TAIR 10
Match: AT4G14570.1 (acylaminoacyl-peptidase-related )

HSP 1 Score: 932.9 bits (2410), Expect = 1.6e-271
Identity = 459/755 (60.79%), Postives = 579/755 (76.69%), Query Frame = 0

Query: 21  ELPAGVDPETEGEYAEQSKLLQEFTTIPNIDKAWMFKSDSGDSVATFSISQASLLSNKRR 80
           EL  G+DP TE EYA QSKLLQEF  IP+IDKAW+F SDSG S A F++SQA+LL+NK++
Sbjct: 11  ELHVGLDPTTEEEYATQSKLLQEFINIPSIDKAWIFNSDSG-SQAMFALSQANLLANKKK 70

Query: 81  KYTLSAHISKGNDDSSVNFKWAPFPVEMIGVSTIVPSPSGSKLLTVRNSENDSPVKLEIW 140
           K+ LS HIS    + SVNF WAPFP+EM G S  VPSPSG KLL +RN EN+SP K EIW
Sbjct: 71  KFMLSGHIS-NESNQSVNFHWAPFPIEMTGASAFVPSPSGLKLLVIRNPENESPTKFEIW 130

Query: 141 SAGQIEKEFHIPQSVHGSIYTDGWFEGISWNSNETYIAYIAEEPSPSKPTFTVSG-YQKG 200
           ++ Q+EKEFHIPQ VHGS+Y DGWFEGISW+S+ET++AY+AEEPS  KPTF   G Y+K 
Sbjct: 131 NSSQLEKEFHIPQKVHGSVYVDGWFEGISWDSDETHVAYVAEEPSRPKPTFDHLGYYKKE 190

Query: 201 CSTNKDSTNWKAQGDFEEEWGEAYAGKRQPALFVINIDSGEVRHVKLIEKYLSVGQVVWA 260
            S +K   +WK +GD+EEEWGEAYAGKRQPALFVIN+DSGEV  +K I + +SVGQVVW+
Sbjct: 191 NSLDKGIGSWKGEGDWEEEWGEAYAGKRQPALFVINVDSGEVEPIKGIPRSISVGQVVWS 250

Query: 261 P-SIGVHQYLVFAGWSSEPRKLGMKYCYNRPCALYAVRAPDYGSEVDEHKLKDGSKEDFP 320
           P S G  QYLVFAGW  + RK G+KYCYNRPCA+YA++           + KD    +FP
Sbjct: 251 PNSNGSAQYLVFAGWLGDKRKFGIKYCYNRPCAIYAIK-------FTSDEPKDDDANEFP 310

Query: 321 LYNLTQNISSAFLPRFSPDGKFLIFLSANSSVNTGAHSATNSLHRINWPTDEKLCFNENI 380
           ++NLT++ISS F PRFS DGKFL+F+SA ++V++GAH AT SLHRI+WP+D KL  + NI
Sbjct: 311 IHNLTKSISSGFCPRFSKDGKFLVFVSAKTAVDSGAHWATESLHRIDWPSDGKLPESTNI 370

Query: 381 VDVVPVVHWAEDDLFPGLYSHGFLINPWLSDGRTVIISSIWHSSEAILSIDILRQFIICF 440
           VDV+ VV+  +D  FPGLY  G L +PWLSDG ++++S+ W S   ILS+++L       
Sbjct: 371 VDVIQVVNCPKDGCFPGLYVTGLLSDPWLSDGHSLMLSTYWRSCRVILSVNLLS------ 430

Query: 441 KFIGEVSRISPSNSNFSWNVLALDGDNVVAVSSSPVDIPQLKYGRLIEKETKNAVWSWLD 500
              GEVSR SPS+S++SWN LALDGD++VAVSSSPV +P++KYG+          W W +
Sbjct: 431 ---GEVSRASPSDSDYSWNALALDGDSIVAVSSSPVSVPEIKYGKKGLDSAGKPSWLWSN 490

Query: 501 VSSPVFKCSEKVVKTLLSALQFSIMKIPVKDVSNCLTKGASKPFEAIFV-SSKSVKGNEL 560
           + SP+ + SEKV+   LS+LQF I+K+P+ DVS  L +GA  P EAI+V SSKS +  + 
Sbjct: 491 IQSPI-RYSEKVMAG-LSSLQFKILKVPISDVSEGLAEGAKNPIEAIYVSSSKSKENGKC 550

Query: 561 NPVIVNLHGGPHSTSISSFSKSSAFLSSLGFNLLVVNYRGSLGFGEDALQSLPGKVGSQD 620
           +P+I  LHGGPHS S  SFS++ A+LSS+G++ L++NYRGSLG+GEDALQSLPGKVGSQD
Sbjct: 551 DPLIAVLHGGPHSVSPCSFSRTMAYLSSIGYSQLIINYRGSLGYGEDALQSLPGKVGSQD 610

Query: 621 VNDVLTALDHIIDKGLANPSKVAVLGGSHGGFLTTHLIGQAPDRFVAAAARNPVCNLALM 680
           V D L A+DH I+ G+A+PS++ VLGGSHGGFLTTHLIGQAPD+FVAAAARNPVCN+A M
Sbjct: 611 VKDCLLAVDHAIEMGIADPSRITVLGGSHGGFLTTHLIGQAPDKFVAAAARNPVCNMASM 670

Query: 681 VGTSDIPDWCYVECYGREWKNYFTEAPSAEHQIELYNKSPILHVSKVKAPTIFLLGAKDL 740
           VG +DIPDWC+ E YG +  +++TEAPSAE     +  SPI H+SKVK PT+FLLG KDL
Sbjct: 671 VGITDIPDWCFFEAYGDQ--SHYTEAPSAEDLSRFHQMSPISHISKVKTPTLFLLGTKDL 730

Query: 741 RVPFSNGLQFARALKEKGVEVKVIMFPDDIHPIDR 773
           RVP SNG Q+ RALKEKGVEVKV++FP+D HP+DR
Sbjct: 731 RVPISNGFQYVRALKEKGVEVKVLVFPNDNHPLDR 743

BLAST of MS007672 vs. TAIR 10
Match: AT5G36210.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 82.4 bits (202), Expect = 1.8e-15
Identity = 56/213 (26.29%), Postives = 102/213 (47.89%), Query Frame = 0

Query: 556 ELNPVIVNLHGGPHSTSISSFSKSSAFLSSLGFNLLVVNYRGSLGFGEDALQSLPGKVGS 615
           E  P++V  HGGP + S  S + +  + +S G+  + VNY GS G+G +  + L  + G 
Sbjct: 490 EKPPLLVKSHGGPTAESRGSLNLNIQYWTSRGWAFVDVNYGGSTGYGREYRERLLRQWGI 549

Query: 616 QDVNDVLTALDHIIDKGLANPSKVAVLGGSHGGFLTTHLIGQAPDRFVAAAARNPVCNLA 675
            DV+D      +++  G A+  ++ + GGS GG+ T   +    D F A A+   V +L 
Sbjct: 550 VDVDDCCGCAKYLVSSGKADVKRLCISGGSAGGYTTLASLA-FRDVFKAGASLYGVADLK 609

Query: 676 LMVGTSDIPDWCYVECYGREWKNYFTEAPSAEHQIELYNKSPILHVSKVKAPTIFLLGAK 735
           ++            +  G ++++ + +    + + + Y +SPI  V K   P I   G +
Sbjct: 610 ML------------KEEGHKFESRYIDNLVGDEK-DFYERSPINFVDKFSCPIILFQGLE 669

Query: 736 DLRVPFSNGLQFARALKEKGVEVKVIMFPDDIH 769
           D  V      +   ALK+KG+ V ++ +  + H
Sbjct: 670 DKVVTPDQSRKIYEALKKKGLPVALVEYEGEQH 688

BLAST of MS007672 vs. TAIR 10
Match: AT1G76140.1 (Prolyl oligopeptidase family protein )

HSP 1 Score: 57.0 bits (136), Expect = 7.9e-08
Identity = 41/131 (31.30%), Postives = 64/131 (48.85%), Query Frame = 0

Query: 547 VSSKSVKGNELNPVIVNLHGGPHSTSISSFSKSSAFLSS-LGFNLLVVNYRGSLGFGED- 606
           V+ K +K +  +P ++  +GG + +   SFS S   LS  LG      N RG   +GE+ 
Sbjct: 533 VAKKDIKLDGSHPCLLYAYGGFNISITPSFSASRIVLSKHLGVVFCFANIRGGGEYGEEW 592

Query: 607 -ALQSLPGKVGSQDVNDVLTALDHIIDKGLANPSKVAVLGGSHGGFLTTHLIGQAPDRFV 666
               SL  K    D  D ++  ++++  G   PSK+ + GGS+GG L    I Q PD + 
Sbjct: 593 HKAGSLAKKQNCFD--DFISGAEYLVSAGYTQPSKLCIEGGSNGGLLVGACINQRPDLYG 652

Query: 667 AAAARNPVCNL 675
            A A   V ++
Sbjct: 653 CALAHVGVMDM 661

BLAST of MS007672 vs. TAIR 10
Match: AT1G76140.2 (Prolyl oligopeptidase family protein )

HSP 1 Score: 57.0 bits (136), Expect = 7.9e-08
Identity = 41/131 (31.30%), Postives = 64/131 (48.85%), Query Frame = 0

Query: 547 VSSKSVKGNELNPVIVNLHGGPHSTSISSFSKSSAFLSS-LGFNLLVVNYRGSLGFGED- 606
           V+ K +K +  +P ++  +GG + +   SFS S   LS  LG      N RG   +GE+ 
Sbjct: 533 VAKKDIKLDGSHPCLLYAYGGFNISITPSFSASRIVLSKHLGVVFCFANIRGGGEYGEEW 592

Query: 607 -ALQSLPGKVGSQDVNDVLTALDHIIDKGLANPSKVAVLGGSHGGFLTTHLIGQAPDRFV 666
               SL  K    D  D ++  ++++  G   PSK+ + GGS+GG L    I Q PD + 
Sbjct: 593 HKAGSLAKKQNCFD--DFISGAEYLVSAGYTQPSKLCIEGGSNGGLLVGACINQRPDLYG 652

Query: 667 AAAARNPVCNL 675
            A A   V ++
Sbjct: 653 CALAHVGVMDM 661

BLAST of MS007672 vs. TAIR 10
Match: AT2G47390.1 (Prolyl oligopeptidase family protein )

HSP 1 Score: 56.2 bits (134), Expect = 1.4e-07
Identity = 41/151 (27.15%), Postives = 68/151 (45.03%), Query Frame = 0

Query: 624 ALDHIIDKGLANPSKVAVLGGSHGGFLTTHLIGQAPDRFVAAAARNPVCNLALM---VGT 683
           A++ ++ +G+A+ SK+AV G S+G F+T +L+  AP  F    AR+   N  L       
Sbjct: 760 AVEEVVRRGVADRSKIAVGGHSYGAFMTANLLAHAPHLFACGIARSGAYNRTLTPFGFQN 819

Query: 684 SDIPDWCYVECYGREWKNYFTEAPSAEHQIELYNKSPILHVSKVKAPTIFLLGAKDLRVP 743
            D   W        E  N + E             SP +  +K+K P + + G +D   P
Sbjct: 820 EDRTLW--------EATNVYVE------------MSPFMSANKIKKPILLIHGEED-NNP 879

Query: 744 FSNGLQ---FARALKEKGVEVKVIMFPDDIH 769
            +  +Q   F  ALK  G   ++++ P + H
Sbjct: 880 GTLTMQSDRFFNALKGHGALCRLVVLPHESH 889

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022138558.10.0e+0098.06acylamino-acid-releasing enzyme isoform X2 [Momordica charantia][more]
XP_022138557.10.0e+0097.43acylamino-acid-releasing enzyme isoform X1 [Momordica charantia][more]
XP_022138559.10.0e+0097.53acylamino-acid-releasing enzyme isoform X3 [Momordica charantia][more]
XP_038875186.10.0e+0087.31acylamino-acid-releasing enzyme isoform X1 [Benincasa hispida][more]
XP_038875189.10.0e+0087.27acylamino-acid-releasing enzyme isoform X2 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
Q84LM42.3e-27060.79Acylamino-acid-releasing enzyme OS=Arabidopsis thaliana OX=3702 GN=AARE PE=1 SV=... [more]
Q0IXP92.1e-25558.31Acylamino-acid-releasing enzyme 1 OS=Oryza sativa subsp. japonica OX=39947 GN=Os... [more]
Q338C03.8e-24957.14Acylamino-acid-releasing enzyme 2 OS=Oryza sativa subsp. japonica OX=39947 GN=Os... [more]
P802274.7e-9032.60Acylamino-acid-releasing enzyme OS=Bos taurus OX=9913 GN=APEH PE=1 SV=2[more]
Q8R1464.0e-8931.90Acylamino-acid-releasing enzyme OS=Mus musculus OX=10090 GN=Apeh PE=1 SV=3[more]
Match NameE-valueIdentityDescription
A0A6J1CDB80.0e+0098.06acylamino-acid-releasing enzyme isoform X2 OS=Momordica charantia OX=3673 GN=LOC... [more]
A0A6J1CBF80.0e+0097.43acylamino-acid-releasing enzyme isoform X1 OS=Momordica charantia OX=3673 GN=LOC... [more]
A0A6J1CAF40.0e+0097.53acylamino-acid-releasing enzyme isoform X3 OS=Momordica charantia OX=3673 GN=LOC... [more]
A0A5A7UVB50.0e+0085.42Acylamino-acid-releasing enzyme OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_... [more]
A0A5D3E4H10.0e+0085.88Acylamino-acid-releasing enzyme OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_... [more]
Match NameE-valueIdentityDescription
AT4G14570.11.6e-27160.79acylaminoacyl-peptidase-related [more]
AT5G36210.11.8e-1526.29alpha/beta-Hydrolases superfamily protein [more]
AT1G76140.17.9e-0831.30Prolyl oligopeptidase family protein [more]
AT1G76140.27.9e-0831.30Prolyl oligopeptidase family protein [more]
AT2G47390.11.4e-0727.15Prolyl oligopeptidase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001375Peptidase S9, prolyl oligopeptidase, catalytic domainPFAMPF00326Peptidase_S9coord: 575..770
e-value: 1.1E-34
score: 119.9
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 505..772
e-value: 3.5E-56
score: 192.8
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 550..774
NoneNo IPR availablePANTHERPTHR42776SERINE PEPTIDASE S9 FAMILY MEMBERcoord: 9..774
NoneNo IPR availablePANTHERPTHR42776:SF24ACYLAMINO-ACID-RELEASING ENZYME-LIKEcoord: 9..774
NoneNo IPR availableSUPERFAMILY82171DPP6 N-terminal domain-likecoord: 105..421
IPR002471Peptidase S9, serine active sitePROSITEPS00708PRO_ENDOPEP_SERcoord: 620..650

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS007672.1MS007672.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
molecular_function GO:0004252 serine-type endopeptidase activity
molecular_function GO:0008236 serine-type peptidase activity