MS006517 (gene) Bitter gourd (TR) v1

BLAST of MS006517 vs. NCBI nr
Match: KAG6588760.1 (hypothetical protein SDJN03_17325, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 196.8 bits (499), Expect = 1.1e-46
Identity = 96/123 (78.05%), Postives = 108/123 (87.80%), Query Frame = 0

Query: 1   MISTVLANIGQRDPILHVTKDAKDGHRSEWIPIRTIEDPYVQEVAKFAVAEFDMEKMANF 60
           MIST+L NIGQ DPILHVTKD K   RSEWI IRTIEDPYVQEVAKFAV EF+++KM  F
Sbjct: 1   MISTLLTNIGQHDPILHVTKDVKGDPRSEWISIRTIEDPYVQEVAKFAVIEFNVDKMTTF 60

Query: 61  QYKRLLRCWIREEEMGGIKYRLILEVKDDAPTPKLGGKYEAIVWEASGRLIIRKLKLISF 120
           QYKR+LRCWI+ EEMGGIKYRL+LEVK +APT K+  KYEA+VWEASGRLIIRKLKL+SF
Sbjct: 61  QYKRMLRCWIK-EEMGGIKYRLVLEVKQEAPTQKV-CKYEAVVWEASGRLIIRKLKLVSF 120

Query: 121 MPC 124
           +PC
Sbjct: 121 VPC 121

BLAST of MS006517 vs. NCBI nr
Match: KAA0031436.1 (Cystatin domain containing protein [Cucumis melo var. makuwa] >TYK06887.1 Cystatin domain containing protein [Cucumis melo var. makuwa])

HSP 1 Score: 194.5 bits (493), Expect = 5.6e-46
Identity = 101/127 (79.53%), Postives = 107/127 (84.25%), Query Frame = 0

Query: 1   MISTVLANIGQRDPILHVT-KDAKDGHRSEWIPIRTIEDPYVQEVAKFAVAEFDMEKMAN 60
           MISTVLANIGQ DPILHVT KD K  H+SEWI IR IEDPYVQEVAKFAV EF+ EKMA 
Sbjct: 1   MISTVLANIGQHDPILHVTSKDTKGDHQSEWISIRAIEDPYVQEVAKFAVREFEKEKMAR 60

Query: 61  FQYKRLLRCWIREEEMGGIKYRLILEVKDDAPTPKLGGKYEAIVWEASGRLIIRKLKLIS 120
            QYKRLL CWI+ EEMGGIKYRLILEV  +    K+ GKYEAIVWEASGRLIIRKLKLIS
Sbjct: 61  SQYKRLLSCWIK-EEMGGIKYRLILEVIKE---EKIEGKYEAIVWEASGRLIIRKLKLIS 120

Query: 121 FMPCLHG 127
           FMPC+HG
Sbjct: 121 FMPCMHG 123

BLAST of MS006517 vs. NCBI nr
Match: KGN43767.1 (hypothetical protein Csa_017208 [Cucumis sativus])

HSP 1 Score: 192.6 bits (488), Expect = 2.1e-45
Identity = 98/128 (76.56%), Postives = 108/128 (84.38%), Query Frame = 0

Query: 1   MISTVLANIGQRDPILHVT-KDAKDGHRSEWIPIRTIEDPYVQEVAKFAVAEFDMEKMAN 60
           MIST+LAN+GQ DPILHVT KD K  H+SEWI IR IEDPYVQEVAKFA+ EF+ EKMA 
Sbjct: 1   MISTLLANVGQHDPILHVTSKDTKGDHQSEWISIRAIEDPYVQEVAKFAMREFEKEKMAR 60

Query: 61  FQYKRLLRCWIREEEMGGIKYRLILEVKDDAPTPKLGGKYEAIVWEASGRLIIRKLKLIS 120
           FQYKRLL CWI+ EEMGGIKYRL+LEV  +    KL GKYEA+VWEASGRLIIRKLKLIS
Sbjct: 61  FQYKRLLSCWIK-EEMGGIKYRLVLEVIKE---EKLEGKYEAVVWEASGRLIIRKLKLIS 120

Query: 121 FMPCLHGA 128
           FMP +HGA
Sbjct: 121 FMPSMHGA 124

BLAST of MS006517 vs. NCBI nr
Match: KAG6571859.1 (hypothetical protein SDJN03_28587, partial [Cucurbita argyrosperma subsp. sororia] >KAG7011544.1 hypothetical protein SDJN02_26450, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 182.2 bits (461), Expect = 2.9e-42
Identity = 92/124 (74.19%), Postives = 99/124 (79.84%), Query Frame = 0

Query: 5   VLANIGQRDPILHVTKDAKDGHRSEWIPIRTIEDPYVQEVAKFAVAEFDMEKMANFQYKR 64
           +LANIGQ DPILHVTKDAK  H SEWI IR IEDPYVQEVAKF V E + EK A FQYK 
Sbjct: 1   MLANIGQHDPILHVTKDAKGDHCSEWISIRAIEDPYVQEVAKFVVVEVNKEKKATFQYKG 60

Query: 65  LLRCWIREEEMGGIKYRLILEVKDDAPTPKLGGKYEAIVWEASGRLIIRKLKLISFMPCL 124
           LLRCW++ EEMGGIKYRLILEVK  A      GKYEAIVWEASGRLIIRKLKL+SF+ C 
Sbjct: 61  LLRCWVK-EEMGGIKYRLILEVKGGASE----GKYEAIVWEASGRLIIRKLKLVSFVACN 119

Query: 125 HGAD 129
           HG +
Sbjct: 121 HGVE 119

BLAST of MS006517 vs. NCBI nr
Match: KAG6571861.1 (Cysteine proteinase inhibitor 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 73.9 bits (180), Expect = 1.1e-09
Identity = 40/108 (37.04%), Postives = 58/108 (53.70%), Query Frame = 0

Query: 1   MISTVLANIGQRDPILHVTKDAKDGHRSEWIPIRTIEDPYVQEVAKFAVAEFDMEKMANF 60
           M S+ + N+GQ DP   V  D KD   S W PI  I++  V+E AK  VAE++ME     
Sbjct: 1   MSSSTIVNVGQYDPGHRVINDLKDCCPSGWTPIPDIDEGCVKEAAKHGVAEYNMEYNETL 60

Query: 61  QYKRLLRCWIREEEMGGIKYRLILEVKDDAPTPKLGGKYEAIVWEASG 109
           +Y+ ++R W  E + GG+ Y  +LE  D         +Y+A+V E  G
Sbjct: 61  KYEGIVRAWYMELKEGGLDYGFVLEAMDCVGRV---NRYKAVVSEDKG 105

BLAST of MS006517 vs. ExPASy Swiss-Prot
Match: Q6TPK4 (Cysteine proteinase inhibitor 1 OS=Actinidia deliciosa OX=3627 PE=1 SV=1)

HSP 1 Score: 52.8 bits (125), Expect = 3.4e-06
Identity = 27/76 (35.53%), Postives = 45/76 (59.21%), Query Frame = 0

Query: 30  WIPIRTIEDPYVQEVAKFAVAEFDMEKMANFQYKRLLRCWIREEEMGGIKYRLILEVKDD 89
           W PI ++    VQ+VA+FAV+E + +     QY+ ++R +   + + G  YRL++  KD 
Sbjct: 32  WRPIESLNSAEVQDVAQFAVSEHNKQANDELQYQSVVRGY--TQVVAGTNYRLVIAAKDG 91

Query: 90  APTPKLGGKYEAIVWE 106
           A    + G YEA+VW+
Sbjct: 92  A----VVGNYEAVVWD 101

BLAST of MS006517 vs. ExPASy Swiss-Prot
Match: P86472 (Cysteine proteinase inhibitor 1 OS=Actinidia chinensis var. chinensis OX=1590841 GN=CYT1 PE=1 SV=2)

HSP 1 Score: 52.4 bits (124), Expect = 4.5e-06
Identity = 27/76 (35.53%), Postives = 44/76 (57.89%), Query Frame = 0

Query: 30  WIPIRTIEDPYVQEVAKFAVAEFDMEKMANFQYKRLLRCWIREEEMGGIKYRLILEVKDD 89
           W PI  +    VQ+VA+FAV+E + +     QY+ ++R +   + + G  YRL++  KD 
Sbjct: 32  WRPIENLNSAEVQDVAQFAVSEHNKQANDELQYQSVVRGY--TQVVAGTNYRLVIAAKDG 91

Query: 90  APTPKLGGKYEAIVWE 106
           A    + G YEA+VW+
Sbjct: 92  A----VVGNYEAVVWD 101

BLAST of MS006517 vs. ExPASy Swiss-Prot
Match: Q10Q47 (Putative cysteine proteinase inhibitor 7 OS=Oryza sativa subsp. japonica OX=39947 GN=Os03g0210100 PE=3 SV=1)

HSP 1 Score: 48.1 bits (113), Expect = 8.4e-05
Identity = 34/94 (36.17%), Postives = 50/94 (53.19%), Query Frame = 0

Query: 30  WIPIRTIEDPYVQEVAKFAVAEFDMEKMA-NFQYKRLLRCWIREEEMGGIKYRLILEVKD 89
           W P+  I DP+VQE+ ++AVAE D    A    ++R+      ++ + G+ YRL LE   
Sbjct: 30  WEPV-DINDPHVQELGRWAVAEEDRGVAAGGLTFERVTDG--EKQVVAGVNYRLTLEASS 89

Query: 90  DAPTPKLGGKYEAIVWEASGRLIIRKLKLISFMP 123
                   G+YEA+V+E   R   R  KL+SF P
Sbjct: 90  SGAK---DGRYEAVVYEQDPRSNAR--KLVSFEP 115

BLAST of MS006517 vs. ExPASy TrEMBL
Match: A0A5A7SN35 (Cystatin domain containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G001920 PE=4 SV=1)

HSP 1 Score: 194.5 bits (493), Expect = 2.7e-46
Identity = 101/127 (79.53%), Postives = 107/127 (84.25%), Query Frame = 0

Query: 1   MISTVLANIGQRDPILHVT-KDAKDGHRSEWIPIRTIEDPYVQEVAKFAVAEFDMEKMAN 60
           MISTVLANIGQ DPILHVT KD K  H+SEWI IR IEDPYVQEVAKFAV EF+ EKMA 
Sbjct: 1   MISTVLANIGQHDPILHVTSKDTKGDHQSEWISIRAIEDPYVQEVAKFAVREFEKEKMAR 60

Query: 61  FQYKRLLRCWIREEEMGGIKYRLILEVKDDAPTPKLGGKYEAIVWEASGRLIIRKLKLIS 120
            QYKRLL CWI+ EEMGGIKYRLILEV  +    K+ GKYEAIVWEASGRLIIRKLKLIS
Sbjct: 61  SQYKRLLSCWIK-EEMGGIKYRLILEVIKE---EKIEGKYEAIVWEASGRLIIRKLKLIS 120

Query: 121 FMPCLHG 127
           FMPC+HG
Sbjct: 121 FMPCMHG 123

BLAST of MS006517 vs. ExPASy TrEMBL
Match: A0A0A0K644 (Cystatin domain containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G066240 PE=4 SV=1)

HSP 1 Score: 192.6 bits (488), Expect = 1.0e-45
Identity = 98/128 (76.56%), Postives = 108/128 (84.38%), Query Frame = 0

Query: 1   MISTVLANIGQRDPILHVT-KDAKDGHRSEWIPIRTIEDPYVQEVAKFAVAEFDMEKMAN 60
           MIST+LAN+GQ DPILHVT KD K  H+SEWI IR IEDPYVQEVAKFA+ EF+ EKMA 
Sbjct: 1   MISTLLANVGQHDPILHVTSKDTKGDHQSEWISIRAIEDPYVQEVAKFAMREFEKEKMAR 60

Query: 61  FQYKRLLRCWIREEEMGGIKYRLILEVKDDAPTPKLGGKYEAIVWEASGRLIIRKLKLIS 120
           FQYKRLL CWI+ EEMGGIKYRL+LEV  +    KL GKYEA+VWEASGRLIIRKLKLIS
Sbjct: 61  FQYKRLLSCWIK-EEMGGIKYRLVLEVIKE---EKLEGKYEAVVWEASGRLIIRKLKLIS 120

Query: 121 FMPCLHGA 128
           FMP +HGA
Sbjct: 121 FMPSMHGA 124

BLAST of MS006517 vs. ExPASy TrEMBL
Match: A0A6J0L5R2 (cysteine proteinase inhibitor 5-like OS=Raphanus sativus OX=3726 GN=LOC108826602 PE=4 SV=1)

HSP 1 Score: 69.3 bits (168), Expect = 1.3e-08
Identity = 38/108 (35.19%), Postives = 57/108 (52.78%), Query Frame = 0

Query: 15  ILHVTKDAKDGHRSEWIPIRTIEDPYVQEVAKFAVAEFDMEKMANFQYKRLLRCWIREEE 74
           +LH +  A  G    W PI  ++DP+V  V KFAV+E+DM+  +  ++  ++      + 
Sbjct: 15  LLHASAAAPTG---RWTPISNVKDPHVVAVGKFAVSEYDMQSKSELKFVAVVSG--ESKV 74

Query: 75  MGGIKYRLILEVKDDAPTPKLGGKYEAIVWEASGRLIIRKLKLISFMP 123
           + GI YRLI+ V D    P     YEAIVWE      ++ + L SF P
Sbjct: 75  VAGISYRLIVAVNDGVAGPGASKNYEAIVWEKEW---LKSMNLTSFKP 114

BLAST of MS006517 vs. ExPASy TrEMBL
Match: A0A0D3E795 (Cysteine proteinase inhibitor OS=Brassica oleracea var. oleracea OX=109376 GN=106317161 PE=4 SV=1)

HSP 1 Score: 67.8 bits (164), Expect = 3.8e-08
Identity = 35/94 (37.23%), Postives = 53/94 (56.38%), Query Frame = 0

Query: 29  EWIPIRTIEDPYVQEVAKFAVAEFDMEKMANFQYKRLLRCWIREEEMGGIKYRLILEVKD 88
           +W PI  ++DP+V EV KFAV+E+DME  +  ++  ++      + + G  YRLI+ V D
Sbjct: 25  KWSPISNVKDPHVVEVGKFAVSEYDMESKSELKFVAVVSG--ESKVVAGTNYRLIVAVND 84

Query: 89  DAPTPKLGGKYEAIVWEASGRLIIRKLKLISFMP 123
               P     YEAIVWE   +  ++ + L SF P
Sbjct: 85  GVAGPGASKNYEAIVWE---KQWLKSMNLTSFKP 113

BLAST of MS006517 vs. ExPASy TrEMBL
Match: A0A2P2NS36 (Cysteine proteinase inhibitor OS=Rhizophora mucronata OX=61149 PE=4 SV=1)

HSP 1 Score: 67.8 bits (164), Expect = 3.8e-08
Identity = 37/93 (39.78%), Postives = 55/93 (59.14%), Query Frame = 0

Query: 30  WIPIRTIEDPYVQEVAKFAVAEFDMEKMANFQYKRLLRCWIREEEMGGIKYRLILEVKDD 89
           W PI  + DP+VQE+A +AV E++    AN + +RL++    ++ + GIKYRL+L VK+ 
Sbjct: 32  WTPITNLSDPHVQEIASYAVREYNHRSNANLKLQRLVKG--EKQVVSGIKYRLVLAVKEG 91

Query: 90  APTPKLGGKYEAIVWEASGRLIIRKLKLISFMP 123
                   KY+A+VWE +        KLISF P
Sbjct: 92  GSDK----KYQAVVWEKAWE---HFRKLISFKP 115