MS006514 (gene) Bitter gourd (TR) v1

Overview
NameMS006514
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionRNase H domain-containing protein
Locationscaffold404: 665718 .. 665990 (-)
RNA-Seq ExpressionMS006514
SyntenyMS006514
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
CTGAAAATTAATACTGACGTTGCAATCAGACTCGATGAAAATCGTATGGGTTTTGGTGCTATGATCCGTAATTCTAAGGGAGAAATTATGCTTACTCTTGCAAAAGAAGTTGGGTGTGTATACGATCCAGCAATAGAAGAGGCGTCGGCGATTAGGGATGGATCGACAGAAGCGATTGAGCTTGGCTTCTTTAATTTGGAGGTGGAGTCGGATTCTCTTCAGGTGATCATGAAGATTAAGAACCCTCAGCCTGACACGATTTGGAGCTCGGTT

mRNA sequence

CTGAAAATTAATACTGACGTTGCAATCAGACTCGATGAAAATCGTATGGGTTTTGGTGCTATGATCCGTAATTCTAAGGGAGAAATTATGCTTACTCTTGCAAAAGAAGTTGGGTGTGTATACGATCCAGCAATAGAAGAGGCGTCGGCGATTAGGGATGGATCGACAGAAGCGATTGAGCTTGGCTTCTTTAATTTGGAGGTGGAGTCGGATTCTCTTCAGGTGATCATGAAGATTAAGAACCCTCAGCCTGACACGATTTGGAGCTCGGTT

Coding sequence (CDS)

CTGAAAATTAATACTGACGTTGCAATCAGACTCGATGAAAATCGTATGGGTTTTGGTGCTATGATCCGTAATTCTAAGGGAGAAATTATGCTTACTCTTGCAAAAGAAGTTGGGTGTGTATACGATCCAGCAATAGAAGAGGCGTCGGCGATTAGGGATGGATCGACAGAAGCGATTGAGCTTGGCTTCTTTAATTTGGAGGTGGAGTCGGATTCTCTTCAGGTGATCATGAAGATTAAGAACCCTCAGCCTGACACGATTTGGAGCTCGGTT

Protein sequence

LKINTDVAIRLDENRMGFGAMIRNSKGEIMLTLAKEVGCVYDPAIEEASAIRDGSTEAIELGFFNLEVESDSLQVIMKIKNPQPDTIWSSV
Homology
BLAST of MS006514 vs. NCBI nr
Match: XP_042964529.1 (uncharacterized protein LOC122298728 [Carya illinoinensis])

HSP 1 Score: 69.3 bits (168), Expect = 1.9e-08
Identity = 32/81 (39.51%), Postives = 55/81 (67.90%), Query Frame = 0

Query: 1   LKINTDVAIRLDENRMGFGAMIRNSKGEIMLTLAKEVGCVYDPAIEEASAIRDGSTEAIE 60
           +K+N D A+R+D N++G G ++R+ + E++ +L K V C++DP++ EA A++       E
Sbjct: 69  VKVNWDAAVRIDLNKVGIGVVVRDEEREVLASLCKVVDCLHDPSVGEALALKRAVQFCDE 128

Query: 61  LGFFNLEVESDSLQVIMKIKN 82
           LGF N+ +E D+ QVI+K  N
Sbjct: 129 LGFSNVVLEGDA-QVIVKAAN 148

BLAST of MS006514 vs. NCBI nr
Match: TXG48983.1 (hypothetical protein EZV62_024858 [Acer yangbiense])

HSP 1 Score: 65.9 bits (159), Expect = 2.1e-07
Identity = 34/80 (42.50%), Postives = 47/80 (58.75%), Query Frame = 0

Query: 2  KINTDVAIRLDENRMGFGAMIRNSKGEIMLTLAKEVGCVYDPAIEEASAIRDGSTEAIEL 61
          KINTD A+ + EN +G   +IRN +G++ML+  K     Y P I EA A+ +G   A   
Sbjct: 3  KINTDAALDIQENYVGIAVVIRNFQGKVMLSFCKNFQACYSPQIAEALAVVEGLRLAKNG 62

Query: 62 GFFNLEVESDSLQVIMKIKN 82
          GFF   +ESD+L V+  I N
Sbjct: 63 GFFPAVLESDALMVVRDICN 82

BLAST of MS006514 vs. NCBI nr
Match: XP_042962682.1 (uncharacterized protein LOC122296954 [Carya illinoinensis])

HSP 1 Score: 65.5 bits (158), Expect = 2.8e-07
Identity = 28/88 (31.82%), Postives = 50/88 (56.82%), Query Frame = 0

Query: 1   LKINTDVAIRLDENRMGFGAMIRNSKGEIMLTLAKEVGCVYDPAIEEASAIRDGSTEAIE 60
           +K+N D A+++ E RMG G +IR+  G++M++L  +   V DP + E  A+R       +
Sbjct: 108 VKVNWDAALKIKEGRMGIGVVIRDENGDVMVSLCSQRRNVRDPLVAELQALRQAMKLCAD 167

Query: 61  LGFFNLEVESDSLQVIMKIKNPQPDTIW 89
           L   ++  E D+L+V+  + NP+    W
Sbjct: 168 LNITDVIFEGDALKVVKAVNNPESSWEW 195

BLAST of MS006514 vs. NCBI nr
Match: XP_018826560.1 (uncharacterized protein LOC108995438 [Juglans regia] >KAF5477039.1 hypothetical protein F2P56_003718 [Juglans regia])

HSP 1 Score: 64.3 bits (155), Expect = 6.2e-07
Identity = 32/88 (36.36%), Postives = 51/88 (57.95%), Query Frame = 0

Query: 2   KINTDVAIRLDENRMGFGAMIRNSKGEIMLTLAKEVGCVYDPAIEEASAIRDGSTEAIEL 61
           K N D AI    +R+G G ++RNS+G +M +L   +  + DP + EA A R  S+  +EL
Sbjct: 79  KANWDAAIDKGNSRLGIGVIVRNSEGSVMASLCSSMDLILDPLLGEAVAARRASSFCVEL 138

Query: 62  GFFNLEVESDSLQVIMKIKNPQPDTIWS 90
           G  ++ +E DSL V+  I++ +    WS
Sbjct: 139 GLQHIILEGDSLSVVKAIQHKEDS--WS 164

BLAST of MS006514 vs. NCBI nr
Match: XP_024039172.1 (uncharacterized protein LOC112097823 [Citrus clementina])

HSP 1 Score: 63.9 bits (154), Expect = 8.1e-07
Identity = 37/82 (45.12%), Postives = 48/82 (58.54%), Query Frame = 0

Query: 2   KINTDVAIRLDENRMGFGAMIRNSKGEIMLTLAKEVGCVYDPAIEEASAIRDGSTEAIEL 61
           K+NTDVA+ +   ++ +GA+IRN +G +ML          D AI EA AIR G   A E 
Sbjct: 50  KLNTDVAVDVQNGKVSYGAVIRNHEGLVMLAGTDFGNYCEDVAIAEAEAIRFGIRLAKET 109

Query: 62  GFFNLEVESDSLQVIMKIKNPQ 84
           G F L +ESDSL VI  I+  Q
Sbjct: 110 GLFPLMIESDSLFVIQLIQGKQ 131

BLAST of MS006514 vs. ExPASy TrEMBL
Match: A0A5C7GW84 (RNase H domain-containing protein OS=Acer yangbiense OX=1000413 GN=EZV62_024858 PE=4 SV=1)

HSP 1 Score: 65.9 bits (159), Expect = 1.0e-07
Identity = 34/80 (42.50%), Postives = 47/80 (58.75%), Query Frame = 0

Query: 2  KINTDVAIRLDENRMGFGAMIRNSKGEIMLTLAKEVGCVYDPAIEEASAIRDGSTEAIEL 61
          KINTD A+ + EN +G   +IRN +G++ML+  K     Y P I EA A+ +G   A   
Sbjct: 3  KINTDAALDIQENYVGIAVVIRNFQGKVMLSFCKNFQACYSPQIAEALAVVEGLRLAKNG 62

Query: 62 GFFNLEVESDSLQVIMKIKN 82
          GFF   +ESD+L V+  I N
Sbjct: 63 GFFPAVLESDALMVVRDICN 82

BLAST of MS006514 vs. ExPASy TrEMBL
Match: A0A2I4F4I0 (uncharacterized protein LOC108995438 OS=Juglans regia OX=51240 GN=LOC108995438 PE=4 SV=1)

HSP 1 Score: 64.3 bits (155), Expect = 3.0e-07
Identity = 32/88 (36.36%), Postives = 51/88 (57.95%), Query Frame = 0

Query: 2   KINTDVAIRLDENRMGFGAMIRNSKGEIMLTLAKEVGCVYDPAIEEASAIRDGSTEAIEL 61
           K N D AI    +R+G G ++RNS+G +M +L   +  + DP + EA A R  S+  +EL
Sbjct: 79  KANWDAAIDKGNSRLGIGVIVRNSEGSVMASLCSSMDLILDPLLGEAVAARRASSFCVEL 138

Query: 62  GFFNLEVESDSLQVIMKIKNPQPDTIWS 90
           G  ++ +E DSL V+  I++ +    WS
Sbjct: 139 GLQHIILEGDSLSVVKAIQHKEDS--WS 164

BLAST of MS006514 vs. ExPASy TrEMBL
Match: A0A5C7GQX3 (RNase H domain-containing protein OS=Acer yangbiense OX=1000413 GN=EZV62_026488 PE=4 SV=1)

HSP 1 Score: 62.4 bits (150), Expect = 1.1e-06
Identity = 32/78 (41.03%), Postives = 45/78 (57.69%), Query Frame = 0

Query: 2   KINTDVAIRLDENRMGFGAMIRNSKGEIMLTLAKEVGCVYDPAIEEASAIRDGSTEAIEL 61
           KINTD A+ +  N  G G +IRN +G++ML+  K     + P I EA A+ +G   A   
Sbjct: 90  KINTDAALNVQGNSAGIGVVIRNFRGQVMLSSCKNFRTAFSPVIAEALAVLEGLRLAKLG 149

Query: 62  GFFNLEVESDSLQVIMKI 80
           GFF   +ESD+L V+  I
Sbjct: 150 GFFPTVLESDALTVVQDI 167

BLAST of MS006514 vs. ExPASy TrEMBL
Match: A0A6A6L732 (RNase H domain-containing protein OS=Hevea brasiliensis OX=3981 GN=GH714_003594 PE=4 SV=1)

HSP 1 Score: 62.4 bits (150), Expect = 1.1e-06
Identity = 32/81 (39.51%), Postives = 49/81 (60.49%), Query Frame = 0

Query: 3   INTDVAIRLDENRMGFGAMIRNSKGEIMLTLAKEVGCVYDPAIEEASAIRDGSTEAIELG 62
           +NTD ++      +G GA+IRN KGE+M+   K +   +DP + E  AI+ G   AI+ G
Sbjct: 68  LNTDASVS-SLGVVGLGAVIRNDKGEVMVASVKSIFANWDPTLAEIHAIKFGLYLAIQTG 127

Query: 63  FFNLEVESDSLQVIMKIKNPQ 84
           F NL+VESD+L  +  ++  Q
Sbjct: 128 FSNLQVESDALGTVDALRGSQ 147

BLAST of MS006514 vs. ExPASy TrEMBL
Match: A0A2P5ENY9 (Ribonuclease H-like domain containing protein OS=Trema orientale OX=63057 GN=TorRG33x02_169030 PE=4 SV=1)

HSP 1 Score: 62.4 bits (150), Expect = 1.1e-06
Identity = 33/76 (43.42%), Postives = 47/76 (61.84%), Query Frame = 0

Query: 1   LKINTDVAIRLDENRMGFGAMIRNSKGEIMLTLAKEVGCVYDPAIEEASAIRDGSTEAIE 60
           LK+N D A+ L E  +G GA+IR+ KG++M  +AK +   +DP I E  AIR+G   A E
Sbjct: 46  LKLNVDAAVNLGEGTIGVGAVIRDYKGDVMGAMAKRIKGSFDPYIAECFAIREGLAFAKE 105

Query: 61  LGFFNLEVESDSLQVI 77
                  VE+DSL+V+
Sbjct: 106 SFLHVRMVETDSLRVV 121

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_042964529.11.9e-0839.51uncharacterized protein LOC122298728 [Carya illinoinensis][more]
TXG48983.12.1e-0742.50hypothetical protein EZV62_024858 [Acer yangbiense][more]
XP_042962682.12.8e-0731.82uncharacterized protein LOC122296954 [Carya illinoinensis][more]
XP_018826560.16.2e-0736.36uncharacterized protein LOC108995438 [Juglans regia] >KAF5477039.1 hypothetical ... [more]
XP_024039172.18.1e-0745.12uncharacterized protein LOC112097823 [Citrus clementina][more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A5C7GW841.0e-0742.50RNase H domain-containing protein OS=Acer yangbiense OX=1000413 GN=EZV62_024858 ... [more]
A0A2I4F4I03.0e-0736.36uncharacterized protein LOC108995438 OS=Juglans regia OX=51240 GN=LOC108995438 P... [more]
A0A5C7GQX31.1e-0641.03RNase H domain-containing protein OS=Acer yangbiense OX=1000413 GN=EZV62_026488 ... [more]
A0A6A6L7321.1e-0639.51RNase H domain-containing protein OS=Hevea brasiliensis OX=3981 GN=GH714_003594 ... [more]
A0A2P5ENY91.1e-0643.42Ribonuclease H-like domain containing protein OS=Trema orientale OX=63057 GN=Tor... [more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 1..90
e-value: 8.4E-6
score: 27.8
IPR002156Ribonuclease H domainPFAMPF13456RVT_3coord: 4..84
e-value: 2.3E-11
score: 43.6
NoneNo IPR availablePANTHERPTHR47074BNAC02G40300D PROTEINcoord: 2..88
NoneNo IPR availablePANTHERPTHR47074:SF25SUBFAMILY NOT NAMEDcoord: 2..88

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS006514.1MS006514.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004523 RNA-DNA hybrid ribonuclease activity