MS006462 (gene) Bitter gourd (TR) v1

BLAST of MS006462 vs. NCBI nr
Match: XP_022154893.1 (gamma-interferon-inducible lysosomal thiol reductase-like [Momordica charantia])

HSP 1 Score: 455.7 bits (1171), Expect = 2.5e-124
Identity = 223/241 (92.53%), Postives = 225/241 (93.36%), Query Frame = 0

Query: 1   MDSRARIPSFACLFHLLLLLCSLVSAFDDSSSITASSNDGSGKVSLDVYYESLCPDSVKF 60
           MDSRARIPSFACLFHLLLLLCSLVSAFDDSSSITA+SNDGSGKVSLDVYYESLCPDSVKF
Sbjct: 1   MDSRARIPSFACLFHLLLLLCSLVSAFDDSSSITANSNDGSGKVSLDVYYESLCPDSVKF 60

Query: 61  IVDDLIKLFEGGLVSIVDLRLVPYGNARVGRNSSITCQHGPSECFLNTVEACAINAWPEL 120
           IVDDLIKLFEGGLVSIVDLRLVPYGNARVGRNSSITCQHGPSECFLNTVEACAINAWPEL
Sbjct: 61  IVDDLIKLFEGGLVSIVDLRLVPYGNARVGRNSSITCQHGPSECFLNTVEACAINAWPEL 120

Query: 121 VSLTTRANWSVFNARHFPFIFCVEAVVYERKYTQWETCYEKLGLDPKPVYECYSSGLGKE 180
                          HFPFIFCVEAVVYERKYTQWETCYEKLGLDPKPVYECYSSGLGKE
Sbjct: 121 TG-------------HFPFIFCVEAVVYERKYTQWETCYEKLGLDPKPVYECYSSGLGKE 180

Query: 181 LELQYAAETDHLQPLHKYVPWVVVDGQPLYEDFENFVSYICKAYRGSYVPSACRAESLSA 240
           LELQYAAETDHLQP HKYVPWVVVDGQPLYEDFENFVSYICKAY+GSYVPSACRAESLSA
Sbjct: 181 LELQYAAETDHLQPPHKYVPWVVVDGQPLYEDFENFVSYICKAYKGSYVPSACRAESLSA 228

Query: 241 I 242
           I
Sbjct: 241 I 228

BLAST of MS006462 vs. NCBI nr
Match: KAG7037011.1 (Gamma-interferon-inducible lysosomal thiol reductase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 392.9 bits (1008), Expect = 2.0e-105
Identity = 194/242 (80.17%), Postives = 211/242 (87.19%), Query Frame = 0

Query: 1   MDSRARIPSFACLFHLLLLLCSLVSAFDDSSSITASSNDGSGKVSLDVYYESLCPDSVKF 60
           MDS ARI SF  L HLLLLLCSLVS    +S  TAS + GS KVSLDVYYESLCPDSVKF
Sbjct: 1   MDSSARISSFVFLSHLLLLLCSLVSI--STSRATASPDYGSEKVSLDVYYESLCPDSVKF 60

Query: 61  IVDDLIKLFEGGLVSIVDLRLVPYGNARVGRNSSITCQHGPSECFLNTVEACAINAWPEL 120
           IVD+LI+LFEGGL+SIVDLR VPYGNAR+ RNSSITCQHGP+EC LNTVEACAI+AWPEL
Sbjct: 61  IVDNLIELFEGGLISIVDLRFVPYGNARISRNSSITCQHGPNECLLNTVEACAIHAWPEL 120

Query: 121 VSLTTRANWSVFNARHFPFIFCVEAVVYERKYTQWETCYEKLGLDPKPVYECYSSGLGKE 180
           VSL  R+ WS     HFPFIFCVEA VYERKYTQWETC+EKLGL PKPVY+CYSSGLGKE
Sbjct: 121 VSLMMRSKWSTGLTGHFPFIFCVEAAVYERKYTQWETCFEKLGLHPKPVYDCYSSGLGKE 180

Query: 181 LELQYAAETDHLQPLHKYVPWVVVDGQPLYEDFENFVSYICKAYRGSYVPSAC-RAESLS 240
           LELQYAAET++LQP HKYVPWVVVDGQPLYEDFENFVSYIC AY+GS VPSAC +A+SLS
Sbjct: 181 LELQYAAETNNLQPPHKYVPWVVVDGQPLYEDFENFVSYICAAYKGSSVPSACSKAKSLS 240

Query: 241 AI 242
           +I
Sbjct: 241 SI 240

BLAST of MS006462 vs. NCBI nr
Match: XP_023523172.1 (gamma-interferon-inducible lysosomal thiol reductase-like [Cucurbita pepo subsp. pepo] >XP_023523173.1 gamma-interferon-inducible lysosomal thiol reductase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 379.4 bits (973), Expect = 2.3e-101
Identity = 190/242 (78.51%), Postives = 206/242 (85.12%), Query Frame = 0

Query: 1   MDSRARIPSFACLFHLLLLLCSLVSAFDDSSSITASSNDGSGKVSLDVYYESLCPDSVKF 60
           MDS ARI SF  L HLLLLLCSLVS    +S  TAS + GS KVSLDVYYESLCPDSVKF
Sbjct: 1   MDSSARISSFVFLSHLLLLLCSLVSV--STSRATASPDYGSEKVSLDVYYESLCPDSVKF 60

Query: 61  IVDDLIKLFEGGLVSIVDLRLVPYGNARVGRNSSITCQHGPSECFLNTVEACAINAWPEL 120
           IVD+LI+LFEGGL+SIVDLR VPYGNAR+GRNSSITCQHGP+EC LNTVEACAI+AWPEL
Sbjct: 61  IVDNLIELFEGGLISIVDLRFVPYGNARIGRNSSITCQHGPNECLLNTVEACAIHAWPEL 120

Query: 121 VSLTTRANWSVFNARHFPFIFCVEAVVYERKYTQWETCYEKLGLDPKPVYECYSSGLGKE 180
                          HFPFIFCVEA VYERKYTQWETC+EKLGL+PKPVY+CYSSGLGKE
Sbjct: 121 TG-------------HFPFIFCVEAAVYERKYTQWETCFEKLGLNPKPVYDCYSSGLGKE 180

Query: 181 LELQYAAETDHLQPLHKYVPWVVVDGQPLYEDFENFVSYICKAYRGSYVPSAC-RAESLS 240
           LELQYAAETD+LQP HKYVPWVVVDGQPLYEDFENFVSYIC AY+GS VPSAC +A+SLS
Sbjct: 181 LELQYAAETDNLQPPHKYVPWVVVDGQPLYEDFENFVSYICAAYKGSSVPSACSKAKSLS 227

Query: 241 AI 242
           +I
Sbjct: 241 SI 227

BLAST of MS006462 vs. NCBI nr
Match: XP_022998319.1 (gamma-interferon-inducible lysosomal thiol reductase-like [Cucurbita maxima] >XP_022998320.1 gamma-interferon-inducible lysosomal thiol reductase-like [Cucurbita maxima] >XP_022998321.1 gamma-interferon-inducible lysosomal thiol reductase-like [Cucurbita maxima] >XP_022998322.1 gamma-interferon-inducible lysosomal thiol reductase-like [Cucurbita maxima])

HSP 1 Score: 375.9 bits (964), Expect = 2.5e-100
Identity = 187/242 (77.27%), Postives = 206/242 (85.12%), Query Frame = 0

Query: 1   MDSRARIPSFACLFHLLLLLCSLVSAFDDSSSITASSNDGSGKVSLDVYYESLCPDSVKF 60
           MDS ARI SF  LFHLLLLLCSLVS    +S  TAS + GS KVSLDVYYESLCPDSVKF
Sbjct: 1   MDSSARISSFVFLFHLLLLLCSLVSV--STSRATASPDYGSEKVSLDVYYESLCPDSVKF 60

Query: 61  IVDDLIKLFEGGLVSIVDLRLVPYGNARVGRNSSITCQHGPSECFLNTVEACAINAWPEL 120
           IVD+LI+LFEGGL+SIVDLR VPYGNAR+ RNSSITCQHGP+EC LNTVEACAI+AWPEL
Sbjct: 61  IVDNLIELFEGGLISIVDLRFVPYGNARINRNSSITCQHGPNECLLNTVEACAIHAWPEL 120

Query: 121 VSLTTRANWSVFNARHFPFIFCVEAVVYERKYTQWETCYEKLGLDPKPVYECYSSGLGKE 180
                          HFPFIFCVEA VYERKYTQWETC+EKLGL+PKPVY+CYSSGLGKE
Sbjct: 121 TG-------------HFPFIFCVEAAVYERKYTQWETCFEKLGLNPKPVYDCYSSGLGKE 180

Query: 181 LELQYAAETDHLQPLHKYVPWVVVDGQPLYEDFENFVSYICKAYRGSYVPSAC-RAESLS 240
           LELQYAA+T++LQP HKYVPWVVVDGQPLYEDFENFVSYIC AY+GS VPSAC +++SLS
Sbjct: 181 LELQYAAQTNNLQPPHKYVPWVVVDGQPLYEDFENFVSYICAAYKGSSVPSACSKSKSLS 227

Query: 241 AI 242
           +I
Sbjct: 241 SI 227

BLAST of MS006462 vs. NCBI nr
Match: XP_022949402.1 (gamma-interferon-inducible lysosomal thiol reductase-like [Cucurbita moschata] >XP_022949403.1 gamma-interferon-inducible lysosomal thiol reductase-like [Cucurbita moschata] >XP_022949404.1 gamma-interferon-inducible lysosomal thiol reductase-like [Cucurbita moschata] >KAG6607335.1 Gamma-interferon-responsive lysosomal thiol protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 374.4 bits (960), Expect = 7.4e-100
Identity = 188/242 (77.69%), Postives = 204/242 (84.30%), Query Frame = 0

Query: 1   MDSRARIPSFACLFHLLLLLCSLVSAFDDSSSITASSNDGSGKVSLDVYYESLCPDSVKF 60
           MDS ARI SF  L HLLLLLCSLVS    +S  TAS + GS KVSLDVYYESLCPDSVKF
Sbjct: 1   MDSSARISSFVFLSHLLLLLCSLVSV--STSRATASPDYGSEKVSLDVYYESLCPDSVKF 60

Query: 61  IVDDLIKLFEGGLVSIVDLRLVPYGNARVGRNSSITCQHGPSECFLNTVEACAINAWPEL 120
           IVD+LI+LFEGGL+SIVDLR VPYGNAR+ RNSSITCQHGP+EC LNTVEACAI+AWPEL
Sbjct: 61  IVDNLIELFEGGLISIVDLRFVPYGNARISRNSSITCQHGPNECLLNTVEACAIHAWPEL 120

Query: 121 VSLTTRANWSVFNARHFPFIFCVEAVVYERKYTQWETCYEKLGLDPKPVYECYSSGLGKE 180
                          HFPFIFCVEA VYERKYTQWETC+EKLGL PKPVY+CYSSGLGKE
Sbjct: 121 TG-------------HFPFIFCVEAAVYERKYTQWETCFEKLGLHPKPVYDCYSSGLGKE 180

Query: 181 LELQYAAETDHLQPLHKYVPWVVVDGQPLYEDFENFVSYICKAYRGSYVPSAC-RAESLS 240
           LELQYAAET++LQP HKYVPWVVVDGQPLYEDFENFVSYIC AY+GS VPSAC +A+SLS
Sbjct: 181 LELQYAAETNNLQPPHKYVPWVVVDGQPLYEDFENFVSYICAAYKGSSVPSACSKAKSLS 227

Query: 241 AI 242
           +I
Sbjct: 241 SI 227

BLAST of MS006462 vs. ExPASy Swiss-Prot
Match: Q9SV73 (Gamma-interferon-responsive lysosomal thiol protein OS=Arabidopsis thaliana OX=3702 GN=GILT PE=2 SV=1)

HSP 1 Score: 162.5 bits (410), Expect = 5.8e-39
Identity = 90/229 (39.30%), Postives = 134/229 (58.52%), Query Frame = 0

Query: 13  LFHLLLLLCSLVSAFDDSSSITASSNDGSGKVSLDVYYESLCPDSVKFIVDDLIKLFEGG 72
           L  L+   C L+  F D  ++ A     SGKV L++YYESLCP   +FIVDDL K+F+  
Sbjct: 6   LTKLVFFGCLLLLTFTD--NLVAGK---SGKVKLNLYYESLCPGCQEFIVDDLGKIFDYD 65

Query: 73  LVSIVDLRLVPYGNARVGRNSSITCQHGPSECFLNTVEACAINAWPELVSLTTRANWSVF 132
           L +I DL+L P+GNA +  N ++TCQHG  EC LN +EACA+  WP+  S          
Sbjct: 66  LYTITDLKLFPFGNAELSDNLTVTCQHGEEECKLNALEACALRTWPDQKS---------- 125

Query: 133 NARHFPFIFCVEAVVYERKYTQWETCYEKLGLDPKPVYECYSSGLGKELELQYAAETDHL 192
               + FI CVE+         WE+C +  G + K + +CY+  L ++L L YA +T +L
Sbjct: 126 ---QYSFIRCVES-----DTKGWESCVKNSGRE-KAINDCYNGDLSRKLILGYATKTKNL 185

Query: 193 QPLHKYVPWVVVDGQPL---YEDFENFVSYICKAYRG-SYVPSACRAES 238
           +P H+YVPWV ++G+PL    +  ++ V+ IC AY+G + +P  C + +
Sbjct: 186 KPPHEYVPWVTLNGKPLDDSVQSTDDLVAQICNAYKGKTTLPKVCNSSA 210

BLAST of MS006462 vs. ExPASy Swiss-Prot
Match: Q499T2 (Gamma-interferon-inducible lysosomal thiol reductase OS=Rattus norvegicus OX=10116 GN=Ifi30 PE=2 SV=1)

HSP 1 Score: 101.7 bits (252), Expect = 1.2e-20
Identity = 68/202 (33.66%), Postives = 101/202 (50.00%), Query Frame = 0

Query: 44  VSLDVYYESLCPDSVKFIVDDLIKLFEGGLVSIVDLRLVPYGNAR---VGRNSSITCQHG 103
           V++ +YYESLC     F+V +L   +   ++ I+++ LVPYGNA+   V      TCQHG
Sbjct: 59  VNVSLYYESLCGACRYFLVRNLFPTWL-MVMEIMNITLVPYGNAQERNVSGTWEFTCQHG 118

Query: 104 PSECFLNTVEACAINAWPELVSLTTRANWSVFNARHFPFIFCVEAVVYERKYTQWETCYE 163
             EC LN VEAC ++   +  +  T              I C+E         + E   +
Sbjct: 119 ELECKLNKVEACLLDKLEKEAAFLT--------------IVCME---------EMEDMEK 178

Query: 164 KLG---------LDPKPVYECYSSGLGKELELQYAAETDHLQPLHKYVPWVVVDGQPLYE 223
           KLG         + P+ + EC +   G EL  + A  TD LQP H+YVPWV+V+ +PL  
Sbjct: 179 KLGPCLQLYVPEVSPESIMECATGKRGTELMHENAQLTDALQPPHEYVPWVLVNEKPL-T 235

Query: 224 DFENFVSYICKAYRGSYVPSAC 234
           D    +S +C+ Y+G+  P  C
Sbjct: 239 DPSQLLSSVCELYQGTEKPDIC 235

BLAST of MS006462 vs. ExPASy Swiss-Prot
Match: A6QPN6 (Gamma-interferon-inducible lysosomal thiol reductase OS=Bos taurus OX=9913 GN=IFI30 PE=2 SV=1)

HSP 1 Score: 101.3 bits (251), Expect = 1.6e-20
Identity = 64/198 (32.32%), Postives = 104/198 (52.53%), Query Frame = 0

Query: 44  VSLDVYYESLCPDSVKFIVDDLIKLFEGGLVSIVDLRLVPYGNAR---VGRNSSITCQHG 103
           V++ +YYE+LCP   +F++ +L   +   ++ I+++ LVPYGNA+   V      TCQHG
Sbjct: 56  VNVSLYYEALCPGCREFLIRELFPTWL-MVLEILNVTLVPYGNAQERNVSGKWEFTCQHG 115

Query: 104 PSECFLNTVEACAINAWPELVSLTTRANWSVFNARHFPFIFCVEAVVYERKYTQWETCYE 163
             EC LN VEAC ++   + ++  T              I C+E +  +      + C +
Sbjct: 116 ERECLLNKVEACLLDQLEQKIAFLT--------------IVCLEEM--DDMEQNLKPCLQ 175

Query: 164 ----KLGLDPKPVYECYSSGLGKELELQYAAETDHLQPLHKYVPWVVVDGQPLYEDFENF 223
               K+  D   + EC +   G +L    A  TD L+P HKYVPWVVV+G+ + +D E+ 
Sbjct: 176 IYAPKVSAD--SIMECATGNRGMQLLHINAQLTDALRPPHKYVPWVVVNGEHM-KDAEHL 232

Query: 224 VSYICKAYRGSYVPSACR 235
           +  +C+ Y+G   P  C+
Sbjct: 236 LHLVCRLYQGQ-KPDVCQ 232

BLAST of MS006462 vs. ExPASy Swiss-Prot
Match: P13284 (Gamma-interferon-inducible lysosomal thiol reductase OS=Homo sapiens OX=9606 GN=IFI30 PE=1 SV=3)

HSP 1 Score: 100.1 bits (248), Expect = 3.5e-20
Identity = 68/202 (33.66%), Postives = 99/202 (49.01%), Query Frame = 0

Query: 44  VSLDVYYESLCPDSVKFIVDDLIKLFEGGLVSIVDLRLVPYGNAR---VGRNSSITCQHG 103
           V++ +YYE+LC     F++ +L   +   ++ I+++ LVPYGNA+   V       CQHG
Sbjct: 62  VNVTLYYEALCGGCRAFLIRELFPTWL-LVMEILNVTLVPYGNAQEQNVSGRWEFKCQHG 121

Query: 104 PSECFLNTVEACAINAWP-ELVSLTTRANWSVFNARHFPFIFCVEAVVYERKYTQWETCY 163
             EC  N VEAC ++    EL  LT               I C+E   +E        C 
Sbjct: 122 EEECKFNKVEACVLDELDMELAFLT---------------IVCMEE--FEDMERSLPLCL 181

Query: 164 EKL--GLDPKPVYECYSSGLGKELELQYAAETDHLQPLHKYVPWVVVDGQPLYEDFENFV 223
           +    GL P  + EC     G +L    A  TD LQP H+YVPWV V+G+PL ED    +
Sbjct: 182 QLYAPGLSPDTIMECAMGDRGMQLMHANAQRTDALQPPHEYVPWVTVNGKPL-EDQTQLL 241

Query: 224 SYICKAYRGSYVPSACRAESLS 240
           + +C+ Y+G   P  C + + S
Sbjct: 242 TLVCQLYQGK-KPDVCPSSTSS 243

BLAST of MS006462 vs. ExPASy Swiss-Prot
Match: E7E2N8 (Gamma-interferon-inducible lysosomal thiol reductase OS=Carassius auratus OX=7957 GN=ifi30 PE=1 SV=1)

HSP 1 Score: 92.8 bits (229), Expect = 5.6e-18
Identity = 63/200 (31.50%), Postives = 97/200 (48.50%), Query Frame = 0

Query: 44  VSLDVYYESLCPDSVKFIVDDLIKLFEGGLVSIVDLRLVPYGNARV---GRNSSITCQHG 103
           V++ +YYESLCP   +F+V  L+  F   L  I+++ LVPYGNA+      N +  CQHG
Sbjct: 58  VTVSLYYESLCPGCREFLVSQLVPTFI-MLSDIMNIELVPYGNAQEKDDQGNYTFICQHG 117

Query: 104 PSECFLNTVEACAINAWPELVSLTTRANWSVFNARHFPFIFCVEAVVYERKYTQWETCYE 163
             EC  N +E C + A                  +  P IFC+E+     K  Q   C  
Sbjct: 118 EDECRGNMIETCLLKA---------------LGPKAIPVIFCMESGADVLKAAQ--PCLG 177

Query: 164 KLGLDP--KPVYECYSSGLGKELELQYAAETDHLQPLHKYVPWVVVDGQPLYEDFE---- 223
               D     V +C +   G +L  Q A +T+ L+P H+YVPW+ ++G+   +D +    
Sbjct: 178 VYFPDTTWDSVMKCVTGDEGNKLMHQNALKTNALKPPHEYVPWITINGEHT-DDLQDKAT 237

Query: 224 -NFVSYICKAYRGSYVPSAC 234
            +  + +C  Y+G   P+AC
Sbjct: 238 NSLFNLVCSLYKGE-KPAAC 237

BLAST of MS006462 vs. ExPASy TrEMBL
Match: A0A6J1DLJ0 (gamma-interferon-inducible lysosomal thiol reductase-like OS=Momordica charantia OX=3673 GN=LOC111022039 PE=3 SV=1)

HSP 1 Score: 455.7 bits (1171), Expect = 1.2e-124
Identity = 223/241 (92.53%), Postives = 225/241 (93.36%), Query Frame = 0

Query: 1   MDSRARIPSFACLFHLLLLLCSLVSAFDDSSSITASSNDGSGKVSLDVYYESLCPDSVKF 60
           MDSRARIPSFACLFHLLLLLCSLVSAFDDSSSITA+SNDGSGKVSLDVYYESLCPDSVKF
Sbjct: 1   MDSRARIPSFACLFHLLLLLCSLVSAFDDSSSITANSNDGSGKVSLDVYYESLCPDSVKF 60

Query: 61  IVDDLIKLFEGGLVSIVDLRLVPYGNARVGRNSSITCQHGPSECFLNTVEACAINAWPEL 120
           IVDDLIKLFEGGLVSIVDLRLVPYGNARVGRNSSITCQHGPSECFLNTVEACAINAWPEL
Sbjct: 61  IVDDLIKLFEGGLVSIVDLRLVPYGNARVGRNSSITCQHGPSECFLNTVEACAINAWPEL 120

Query: 121 VSLTTRANWSVFNARHFPFIFCVEAVVYERKYTQWETCYEKLGLDPKPVYECYSSGLGKE 180
                          HFPFIFCVEAVVYERKYTQWETCYEKLGLDPKPVYECYSSGLGKE
Sbjct: 121 TG-------------HFPFIFCVEAVVYERKYTQWETCYEKLGLDPKPVYECYSSGLGKE 180

Query: 181 LELQYAAETDHLQPLHKYVPWVVVDGQPLYEDFENFVSYICKAYRGSYVPSACRAESLSA 240
           LELQYAAETDHLQP HKYVPWVVVDGQPLYEDFENFVSYICKAY+GSYVPSACRAESLSA
Sbjct: 181 LELQYAAETDHLQPPHKYVPWVVVDGQPLYEDFENFVSYICKAYKGSYVPSACRAESLSA 228

Query: 241 I 242
           I
Sbjct: 241 I 228

BLAST of MS006462 vs. ExPASy TrEMBL
Match: A0A6J1KC63 (gamma-interferon-inducible lysosomal thiol reductase-like OS=Cucurbita maxima OX=3661 GN=LOC111492992 PE=3 SV=1)

HSP 1 Score: 375.9 bits (964), Expect = 1.2e-100
Identity = 187/242 (77.27%), Postives = 206/242 (85.12%), Query Frame = 0

Query: 1   MDSRARIPSFACLFHLLLLLCSLVSAFDDSSSITASSNDGSGKVSLDVYYESLCPDSVKF 60
           MDS ARI SF  LFHLLLLLCSLVS    +S  TAS + GS KVSLDVYYESLCPDSVKF
Sbjct: 1   MDSSARISSFVFLFHLLLLLCSLVSV--STSRATASPDYGSEKVSLDVYYESLCPDSVKF 60

Query: 61  IVDDLIKLFEGGLVSIVDLRLVPYGNARVGRNSSITCQHGPSECFLNTVEACAINAWPEL 120
           IVD+LI+LFEGGL+SIVDLR VPYGNAR+ RNSSITCQHGP+EC LNTVEACAI+AWPEL
Sbjct: 61  IVDNLIELFEGGLISIVDLRFVPYGNARINRNSSITCQHGPNECLLNTVEACAIHAWPEL 120

Query: 121 VSLTTRANWSVFNARHFPFIFCVEAVVYERKYTQWETCYEKLGLDPKPVYECYSSGLGKE 180
                          HFPFIFCVEA VYERKYTQWETC+EKLGL+PKPVY+CYSSGLGKE
Sbjct: 121 TG-------------HFPFIFCVEAAVYERKYTQWETCFEKLGLNPKPVYDCYSSGLGKE 180

Query: 181 LELQYAAETDHLQPLHKYVPWVVVDGQPLYEDFENFVSYICKAYRGSYVPSAC-RAESLS 240
           LELQYAA+T++LQP HKYVPWVVVDGQPLYEDFENFVSYIC AY+GS VPSAC +++SLS
Sbjct: 181 LELQYAAQTNNLQPPHKYVPWVVVDGQPLYEDFENFVSYICAAYKGSSVPSACSKSKSLS 227

Query: 241 AI 242
           +I
Sbjct: 241 SI 227

BLAST of MS006462 vs. ExPASy TrEMBL
Match: A0A6J1GC05 (gamma-interferon-inducible lysosomal thiol reductase-like OS=Cucurbita moschata OX=3662 GN=LOC111452764 PE=3 SV=1)

HSP 1 Score: 374.4 bits (960), Expect = 3.6e-100
Identity = 188/242 (77.69%), Postives = 204/242 (84.30%), Query Frame = 0

Query: 1   MDSRARIPSFACLFHLLLLLCSLVSAFDDSSSITASSNDGSGKVSLDVYYESLCPDSVKF 60
           MDS ARI SF  L HLLLLLCSLVS    +S  TAS + GS KVSLDVYYESLCPDSVKF
Sbjct: 1   MDSSARISSFVFLSHLLLLLCSLVSV--STSRATASPDYGSEKVSLDVYYESLCPDSVKF 60

Query: 61  IVDDLIKLFEGGLVSIVDLRLVPYGNARVGRNSSITCQHGPSECFLNTVEACAINAWPEL 120
           IVD+LI+LFEGGL+SIVDLR VPYGNAR+ RNSSITCQHGP+EC LNTVEACAI+AWPEL
Sbjct: 61  IVDNLIELFEGGLISIVDLRFVPYGNARISRNSSITCQHGPNECLLNTVEACAIHAWPEL 120

Query: 121 VSLTTRANWSVFNARHFPFIFCVEAVVYERKYTQWETCYEKLGLDPKPVYECYSSGLGKE 180
                          HFPFIFCVEA VYERKYTQWETC+EKLGL PKPVY+CYSSGLGKE
Sbjct: 121 TG-------------HFPFIFCVEAAVYERKYTQWETCFEKLGLHPKPVYDCYSSGLGKE 180

Query: 181 LELQYAAETDHLQPLHKYVPWVVVDGQPLYEDFENFVSYICKAYRGSYVPSAC-RAESLS 240
           LELQYAAET++LQP HKYVPWVVVDGQPLYEDFENFVSYIC AY+GS VPSAC +A+SLS
Sbjct: 181 LELQYAAETNNLQPPHKYVPWVVVDGQPLYEDFENFVSYICAAYKGSSVPSACSKAKSLS 227

Query: 241 AI 242
           +I
Sbjct: 241 SI 227

BLAST of MS006462 vs. ExPASy TrEMBL
Match: A0A0A0LVM7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G533550 PE=3 SV=1)

HSP 1 Score: 352.8 bits (904), Expect = 1.1e-93
Identity = 176/235 (74.89%), Postives = 196/235 (83.40%), Query Frame = 0

Query: 7   IPSFACLFHLLLLLCSLVSAFDDSSSITASSNDGSGKVSLDVYYESLCPDSVKFIVDDLI 66
           +PSF  LFHLLLLLCSLVSA   S  I    N G  KVSLDVYYESLCPDSVKFIVD+LI
Sbjct: 8   VPSFLSLFHLLLLLCSLVSA---SPPI---PNYGCEKVSLDVYYESLCPDSVKFIVDNLI 67

Query: 67  KLFEGGLVSIVDLRLVPYGNARVGRNSSITCQHGPSECFLNTVEACAINAWPELVSLTTR 126
           +LFEG L+SIVDLR VPYGNAR+ RNSSITCQHGP+EC LNTVEACAI+AWP+L   T  
Sbjct: 68  ELFEGDLLSIVDLRFVPYGNARLDRNSSITCQHGPNECLLNTVEACAIHAWPDLTGAT-- 127

Query: 127 ANWSVFNARHFPFIFCVEAVVYERKYTQWETCYEKLGLDPKPVYECYSSGLGKELELQYA 186
                    HFPFI+CV+ VVYERKYTQW+TC+EKLGL+ KPVY+CYSSGLGKELELQYA
Sbjct: 128 --------GHFPFIYCVQTVVYERKYTQWKTCFEKLGLNSKPVYDCYSSGLGKELELQYA 187

Query: 187 AETDHLQPLHKYVPWVVVDGQPLYEDFENFVSYICKAYRGSYVPSACRAESLSAI 242
           AET++LQP HKYVPWVVVDGQPLYED+ENFVSYIC AY+G  VP+ACRA+SLS I
Sbjct: 188 AETNNLQPPHKYVPWVVVDGQPLYEDYENFVSYICAAYKGFSVPTACRAKSLSDI 226

BLAST of MS006462 vs. ExPASy TrEMBL
Match: A0A1S3C5X1 (gamma-interferon-inducible lysosomal thiol reductase-like OS=Cucumis melo OX=3656 GN=LOC103497041 PE=3 SV=1)

HSP 1 Score: 351.3 bits (900), Expect = 3.2e-93
Identity = 172/235 (73.19%), Postives = 193/235 (82.13%), Query Frame = 0

Query: 7   IPSFACLFHLLLLLCSLVSAFDDSSSITASSNDGSGKVSLDVYYESLCPDSVKFIVDDLI 66
           IPSF CLFHLLLLLCSLVSA        +S N G  KVSLDVYYESLCPDSVKFIVD+LI
Sbjct: 8   IPSFLCLFHLLLLLCSLVSA------SPSSPNYGCEKVSLDVYYESLCPDSVKFIVDNLI 67

Query: 67  KLFEGGLVSIVDLRLVPYGNARVGRNSSITCQHGPSECFLNTVEACAINAWPELVSLTTR 126
           +LFEG L+ IVDLR VPYGNAR+ RNSSITCQHGP+EC LNTVEACAI+ WP+L   T  
Sbjct: 68  ELFEGDLLPIVDLRFVPYGNARLDRNSSITCQHGPNECLLNTVEACAIHVWPDLTGAT-- 127

Query: 127 ANWSVFNARHFPFIFCVEAVVYERKYTQWETCYEKLGLDPKPVYECYSSGLGKELELQYA 186
                    HFPFI+CV  +VYERKYTQW+TC+EKLGL+ KP+ +CYSSGLGKELELQYA
Sbjct: 128 --------GHFPFIYCVGTLVYERKYTQWKTCFEKLGLNSKPISDCYSSGLGKELELQYA 187

Query: 187 AETDHLQPLHKYVPWVVVDGQPLYEDFENFVSYICKAYRGSYVPSACRAESLSAI 242
           AET++LQP HKYVPWVVVDGQPLYED+ENFVSYIC AY+GS VP+ACRA+S S I
Sbjct: 188 AETNNLQPPHKYVPWVVVDGQPLYEDYENFVSYICAAYKGSAVPTACRAKSFSGI 226

BLAST of MS006462 vs. TAIR 10
Match: AT1G07080.1 (Thioredoxin superfamily protein )

HSP 1 Score: 250.8 bits (639), Expect = 1.1e-66
Identity = 125/208 (60.10%), Postives = 149/208 (71.63%), Query Frame = 0

Query: 35  ASSNDGSG--------KVSLDVYYESLCPDSVKFIVDDLIKLFEGGLVSIVDLRLVPYGN 94
           ASS+D SG        KVS+ +YYESLCP    FIV+ L KLFE  L+SIVDL L P+GN
Sbjct: 22  ASSSDYSGVSLPSSSPKVSVGLYYESLCPYCSSFIVNHLAKLFEDDLISIVDLHLSPWGN 81

Query: 95  ARV-GRNSSITCQHGPSECFLNTVEACAINAWPELVSLTTRANWSVFNARHFPFIFCVEA 154
            ++   N +  CQHG  ECFL+TVEACAI+AWP++             + HFPFI+CVE 
Sbjct: 82  TKLRSDNVTAVCQHGAFECFLDTVEACAIDAWPKV-------------SDHFPFIYCVEK 141

Query: 155 VVYERKYTQWETCYEKLGLDPKPVYECYSSGLGKELELQYAAETDHLQPLHKYVPWVVVD 214
           +V E KY +WETCYEKL L+ KPV +C SSG G EL L YAAET+ LQP HKYVPWVVVD
Sbjct: 142 LVTEHKYDKWETCYEKLNLNSKPVADCLSSGHGNELALHYAAETNALQPPHKYVPWVVVD 201

Query: 215 GQPLYEDFENFVSYICKAYRGSYVPSAC 234
           GQPLYED+ENF+SYICKAY+G+ VP AC
Sbjct: 202 GQPLYEDYENFISYICKAYKGNKVPGAC 216

BLAST of MS006462 vs. TAIR 10
Match: AT5G01580.1 (gamma interferon responsive lysosomal thiol (GILT) reductase family protein )

HSP 1 Score: 199.5 bits (506), Expect = 3.0e-51
Identity = 92/198 (46.46%), Postives = 130/198 (65.66%), Query Frame = 0

Query: 37  SNDGSGKVSLDVYYESLCPDSVKFIVDDLIKLFEGGLVSIVDLRLVPYGNARVGRNSSIT 96
           S   S KV+L +YYE+LCP   +FIV+ L K+FE GL+S +DL+LVP+GNA +  + +I 
Sbjct: 20  SLSSSQKVTLSLYYEALCPFCAEFIVNRLPKIFETGLISSIDLQLVPWGNAAIRPDGTIL 79

Query: 97  CQHGPSECFLNTVEACAINAWPELVSLTTRANWSVFNARHFPFIFCVEAVVYERKYTQWE 156
           CQHG +EC LN + ACAINA+P+++             +HF +I+C E +V E K  +W 
Sbjct: 80  CQHGEAECALNAIHACAINAYPDVM-------------KHFGYIYCTEQLVLENKLEKWA 139

Query: 157 TCYEKLGLDPKPVYECYSSGLGKELELQYAAETDHLQPLHKYVPWVVVDGQPLYEDFENF 216
            C E +GL  +   +CY +G G +LE +YA ET  L P H++VPWVVV+  PL E+++NF
Sbjct: 140 DCLEMVGLS-RAAVDCYINGYGNQLEQRYAEETSELYPAHRFVPWVVVNNLPLQENYQNF 199

Query: 217 VSYICKAYRGSYVPSACR 235
           V Y+C AY  + VP ACR
Sbjct: 200 VMYVCNAYGSNQVPEACR 203

BLAST of MS006462 vs. TAIR 10
Match: AT4G12890.1 (Gamma interferon responsive lysosomal thiol (GILT) reductase family protein )

HSP 1 Score: 181.8 bits (460), Expect = 6.5e-46
Identity = 93/229 (40.61%), Postives = 134/229 (58.52%), Query Frame = 0

Query: 10  FACLFHLLLLLCSLVSAFDDSSSITASSNDGSGKVSLDVYYESLCPDSVKFIVDDLIKLF 69
           F  LF  L L C  V  + ++  +  +SN    KV +++YYESLCP    FIVDDL K+F
Sbjct: 9   FVDLFPCLFLACLFVFTYSNNLVVAENSN----KVKINLYYESLCPYCQNFIVDDLGKIF 68

Query: 70  EGGLVSIVDLRLVPYGNARVGRNSSITCQHGPSECFLNTVEACAINAWPELVSLTTRANW 129
           +  L+ I DL+LVP+GNA +  N +ITCQHG  EC LN +EAC I   P+          
Sbjct: 69  DSDLLKITDLKLVPFGNAHISNNLTITCQHGEEECKLNALEACGIRTLPD---------- 128

Query: 130 SVFNARHFPFIFCVEAVVYERKYTQWETCYEKLGLDPKPVYECYSSGLGKELELQYAAET 189
                  + FI CV     E+   +WE+C +K G + K + +CY+  L ++L L YA  T
Sbjct: 129 ---PKLQYKFIRCV-----EKDTNEWESCVKKSGRE-KAINDCYNGDLSQKLILGYAKLT 188

Query: 190 DHLQPLHKYVPWVVVDGQPLYEDFENFVSYICKAYRGSYVPSACRAESL 239
             L+P H+YVPWV ++G+PLY+++ N V+ +CKAY+G  +P  C +  L
Sbjct: 189 SSLKPKHEYVPWVTLNGKPLYDNYHNLVAQVCKAYKGKDLPKLCSSSVL 214

BLAST of MS006462 vs. TAIR 10
Match: AT4G12900.1 (Gamma interferon responsive lysosomal thiol (GILT) reductase family protein )

HSP 1 Score: 175.6 bits (444), Expect = 4.7e-44
Identity = 97/232 (41.81%), Postives = 132/232 (56.90%), Query Frame = 0

Query: 9   SFACLFHLLLLLCSLVSAFDDSSSITASSNDGSGKVSLDVYYESLCPDSVKFIVDDLIKL 68
           S A    L+   C L+  F  S ++ A  +D   KV L++YYESLCP    FIV  L K+
Sbjct: 6   SLASSTKLVFFACLLLFTF-SSHNLVAGESD---KVKLNLYYESLCPSCQNFIVHHLGKI 65

Query: 69  FEGGLVSIVDLRLVPYGNARVGRNSSITCQHGPSECFLNTVEACAINAWPELVSLTTRAN 128
           F   L +I DL+L+P+GNA V  + ++TCQHG  EC LN +EACAI  WP          
Sbjct: 66  FNTDLHTITDLKLIPFGNAHVSDDLTVTCQHGEEECKLNALEACAIRTWPN--------- 125

Query: 129 WSVFNARHFPFIFCVEAVVYERKYTQWETCYEKLGLDPKPVYECYSSGLGKELELQYAAE 188
                  H+ FI CVE          WE+C +K G   K + +CY+  L KEL L YA +
Sbjct: 126 ----QRLHYKFIRCVET-----NTNAWESCVKKYG-GEKAINDCYNGDLSKELILGYANQ 185

Query: 189 TDHLQPLHKYVPWVVVDGQPLYEDFENFVSYICKAYRG-SYVPSACRAESLS 240
           T  L+P HKYVPW+ ++G+PLYE+  +FV  +CKAY+G + +P  C +  LS
Sbjct: 186 TLSLKPEHKYVPWMTLNGEPLYENIGDFVDLVCKAYKGKAALPKLCYSSVLS 214

BLAST of MS006462 vs. TAIR 10
Match: AT4G12960.1 (Gamma interferon responsive lysosomal thiol (GILT) reductase family protein )

HSP 1 Score: 162.5 bits (410), Expect = 4.1e-40
Identity = 90/229 (39.30%), Postives = 134/229 (58.52%), Query Frame = 0

Query: 13  LFHLLLLLCSLVSAFDDSSSITASSNDGSGKVSLDVYYESLCPDSVKFIVDDLIKLFEGG 72
           L  L+   C L+  F D  ++ A     SGKV L++YYESLCP   +FIVDDL K+F+  
Sbjct: 6   LTKLVFFGCLLLLTFTD--NLVAGK---SGKVKLNLYYESLCPGCQEFIVDDLGKIFDYD 65

Query: 73  LVSIVDLRLVPYGNARVGRNSSITCQHGPSECFLNTVEACAINAWPELVSLTTRANWSVF 132
           L +I DL+L P+GNA +  N ++TCQHG  EC LN +EACA+  WP+  S          
Sbjct: 66  LYTITDLKLFPFGNAELSDNLTVTCQHGEEECKLNALEACALRTWPDQKS---------- 125

Query: 133 NARHFPFIFCVEAVVYERKYTQWETCYEKLGLDPKPVYECYSSGLGKELELQYAAETDHL 192
               + FI CVE+         WE+C +  G + K + +CY+  L ++L L YA +T +L
Sbjct: 126 ---QYSFIRCVES-----DTKGWESCVKNSGRE-KAINDCYNGDLSRKLILGYATKTKNL 185

Query: 193 QPLHKYVPWVVVDGQPL---YEDFENFVSYICKAYRG-SYVPSACRAES 238
           +P H+YVPWV ++G+PL    +  ++ V+ IC AY+G + +P  C + +
Sbjct: 186 KPPHEYVPWVTLNGKPLDDSVQSTDDLVAQICNAYKGKTTLPKVCNSSA 210