MS006250 (gene) Bitter gourd (TR) v1

Overview


Name	MS006250
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Membrane-associated kinase regulator 5
Location	scaffold96: 1578001 .. 1578822 (+)
RNA-Seq Expression	MS006250
Synteny	MS006250

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATTCTGTTGCCTTTCTAAAGTTCTGGAAAATCGCCGCCTGCCCCATCACCACTAACAACGCCATTTTTTCCACCGCCGACGAACAAGAACAGCAAAAAGAAGAAGAAGAAGAAGAAGAAGATTCGTTCATCGATCTCGAGCTCCTCTCTGTTTCCGCTGCCGAAGCCCAGAGACCTCAAATTCCGACCAAATTAGGCGACCACATAAACCTCTGCTCCCGGGATTTACAGACTGATTCCATTTCCAAGCGGAAGATTCTCCCGATCGACCAGAGCCCCAAGCCGCAGTCGCCGATTCAGCTTCTGAAATCGGCGCCCAAGTTCCCGGTCTTCATCTTCAAGAAGCAGAAATCAATGGCGAAAAACAGAGCAGCCGCCGGATCAGAGCCGGCGAGGGGAGAACAAGAAGAAGAAGAAAACAGAATTCCGAGCTCGAAACTCGCCAGAGATAACAGCACGAGAAGAATCGGAGGCCTTTTTCCGGCCGACCCATCTCCGGCGCCGGCGAGGCGATTCTCCAAGGAGACGGTGCAGAAATATCTGAAGCTGATTAAGCCTAAAGCTCTCAAGAAACCCGCCGGCGACGATCCGCCGCCGGATTCTCCGATGAGGGAAAAGCAGGAGAATAGTTTTCCGGCGGGGATCCGGCTGGTGTGTAAGCATCTGGGTAAGAGCAAATCGGCGTCATCGTCGGCGACGGGCGCGCCACCGCCGAACCGGAGAGACGATTCATTGTTACAGCAACACGACGGGATTCAGAGCGCCATTTTACACTGCAAAAGATCCTTCAACTCTTCTACAGGTAAAAGTACGAAACTA

mRNA sequence

ATGGATTCTGTTGCCTTTCTAAAGTTCTGGAAAATCGCCGCCTGCCCCATCACCACTAACAACGCCATTTTTTCCACCGCCGACGAACAAGAACAGCAAAAAGAAGAAGAAGAAGAAGAAGAAGATTCGTTCATCGATCTCGAGCTCCTCTCTGTTTCCGCTGCCGAAGCCCAGAGACCTCAAATTCCGACCAAATTAGGCGACCACATAAACCTCTGCTCCCGGGATTTACAGACTGATTCCATTTCCAAGCGGAAGATTCTCCCGATCGACCAGAGCCCCAAGCCGCAGTCGCCGATTCAGCTTCTGAAATCGGCGCCCAAGTTCCCGGTCTTCATCTTCAAGAAGCAGAAATCAATGGCGAAAAACAGAGCAGCCGCCGGATCAGAGCCGGCGAGGGGAGAACAAGAAGAAGAAGAAAACAGAATTCCGAGCTCGAAACTCGCCAGAGATAACAGCACGAGAAGAATCGGAGGCCTTTTTCCGGCCGACCCATCTCCGGCGCCGGCGAGGCGATTCTCCAAGGAGACGGTGCAGAAATATCTGAAGCTGATTAAGCCTAAAGCTCTCAAGAAACCCGCCGGCGACGATCCGCCGCCGGATTCTCCGATGAGGGAAAAGCAGGAGAATAGTTTTCCGGCGGGGATCCGGCTGGTGTGTAAGCATCTGGGTAAGAGCAAATCGGCGTCATCGTCGGCGACGGGCGCGCCACCGCCGAACCGGAGAGACGATTCATTGTTACAGCAACACGACGGGATTCAGAGCGCCATTTTACACTGCAAAAGATCCTTCAACTCTTCTACAGGTAAAAGTACGAAACTA

Coding sequence (CDS)

ATGGATTCTGTTGCCTTTCTAAAGTTCTGGAAAATCGCCGCCTGCCCCATCACCACTAACAACGCCATTTTTTCCACCGCCGACGAACAAGAACAGCAAAAAGAAGAAGAAGAAGAAGAAGAAGATTCGTTCATCGATCTCGAGCTCCTCTCTGTTTCCGCTGCCGAAGCCCAGAGACCTCAAATTCCGACCAAATTAGGCGACCACATAAACCTCTGCTCCCGGGATTTACAGACTGATTCCATTTCCAAGCGGAAGATTCTCCCGATCGACCAGAGCCCCAAGCCGCAGTCGCCGATTCAGCTTCTGAAATCGGCGCCCAAGTTCCCGGTCTTCATCTTCAAGAAGCAGAAATCAATGGCGAAAAACAGAGCAGCCGCCGGATCAGAGCCGGCGAGGGGAGAACAAGAAGAAGAAGAAAACAGAATTCCGAGCTCGAAACTCGCCAGAGATAACAGCACGAGAAGAATCGGAGGCCTTTTTCCGGCCGACCCATCTCCGGCGCCGGCGAGGCGATTCTCCAAGGAGACGGTGCAGAAATATCTGAAGCTGATTAAGCCTAAAGCTCTCAAGAAACCCGCCGGCGACGATCCGCCGCCGGATTCTCCGATGAGGGAAAAGCAGGAGAATAGTTTTCCGGCGGGGATCCGGCTGGTGTGTAAGCATCTGGGTAAGAGCAAATCGGCGTCATCGTCGGCGACGGGCGCGCCACCGCCGAACCGGAGAGACGATTCATTGTTACAGCAACACGACGGGATTCAGAGCGCCATTTTACACTGCAAAAGATCCTTCAACTCTTCTACAGGTAAAAGTACGAAACTA

Protein sequence

MDSVAFLKFWKIAACPITTNNAIFSTADEQEQQKEEEEEEEDSFIDLELLSVSAAEAQRPQIPTKLGDHINLCSRDLQTDSISKRKILPIDQSPKPQSPIQLLKSAPKFPVFIFKKQKSMAKNRAAAGSEPARGEQEEEENRIPSSKLARDNSTRRIGGLFPADPSPAPARRFSKETVQKYLKLIKPKALKKPAGDDPPPDSPMREKQENSFPAGIRLVCKHLGKSKSASSSATGAPPPNRRDDSLLQQHDGIQSAILHCKRSFNSSTGKSTKL

Homology

[Close]

BLAST of MS006250 vs. NCBI nr
Match: XP_008437543.1 (PREDICTED: probable membrane-associated kinase regulator 5 [Cucumis melo])

HSP 1 Score: 319.7 bits (818), Expect = 2.5e-83
Identity = 188/294 (63.95%), Postives = 215/294 (73.13%), Query Frame = 0


Query: 1   MDSVAFLKFWKIAACPITTNNAIFSTADEQEQQKEEEEEEEDSFIDLELLSVSAAE---- 60
           MDSVAFLKF+K        + A      E+E+++EEEEEEEDSF D+E  SVSA +    
Sbjct: 1   MDSVAFLKFFK-------HSGADELQKQEEEEEEEEEEEEEDSFFDIEFFSVSAGKIQSP 60

Query: 61  AQRPQIPTKLGDHINLCSRDLQTDSISKRKILPIDQSPKPQSPIQLLKSAPKFPVFIFKK 120
            QRPQIPTKLGDHINLCS     DSISKRKILPI+ + KPQSPIQLLKSAPKFPVFIFKK
Sbjct: 61  EQRPQIPTKLGDHINLCS-----DSISKRKILPIEPTSKPQSPIQLLKSAPKFPVFIFKK 120

Query: 121 QKSMAKNRAAAGSEPARGEQEEEENRIPSSKLARDNSTRRIGGLFPADPS----PAPARR 180
           Q+SMAKN+A  G       QE +++ +PSSKL RDNSTRR GG+FP+DP     PAP RR
Sbjct: 121 QRSMAKNKAPTG-------QEGDQSGVPSSKLTRDNSTRRFGGIFPSDPCEPPVPAPTRR 180

Query: 181 FSKETVQKYLKLIKPKALKKP--AGDD------PPPDSPMREKQENSFPAGIRLVCKHLG 240
           FSKE V+KY KLIKPK L+KP  +G D         DSP+REK EN  PAGIRLVC+HLG
Sbjct: 181 FSKEAVRKYFKLIKPKVLRKPTDSGSDLSSSSAAAVDSPVREKHENGLPAGIRLVCRHLG 240

Query: 241 KSKSASS----SATGAPPPNRRDDSLLQQHDGIQSAILHCKRSFNSSTGKSTKL 275
           KSKSAS+    +A   PPPNRRDDSLLQQHDGIQSAILHCKRSFN+S+ K   L
Sbjct: 241 KSKSASAAGGLAAAVVPPPNRRDDSLLQQHDGIQSAILHCKRSFNASSEKPLSL 275

[Close]

BLAST of MS006250 vs. NCBI nr
Match: XP_038906856.1 (probable membrane-associated kinase regulator 5 [Benincasa hispida])

HSP 1 Score: 319.7 bits (818), Expect = 2.5e-83
Identity = 190/290 (65.52%), Postives = 211/290 (72.76%), Query Frame = 0


Query: 1   MDSVAFLKFWKIAACPITTNNAIFSTADEQEQQKEEEEEEEDSFIDLELLSVSAAE---- 60
           MDSVAFLK +K++                 E QK EEEEEEDSF DLE  SVSA +    
Sbjct: 1   MDSVAFLKLFKLSGA--------------DELQKHEEEEEEDSFFDLEFFSVSAGKIQSP 60

Query: 61  AQRPQIPTKLGDHINLCSRDLQTDSISKRKILPIDQSPKPQSPIQLLKSAPKFPVFIFKK 120
            Q+PQIPTKLGDHINLCS     DSISKRKILPI+ + KPQSPIQLLKSAPKFPVFIFKK
Sbjct: 61  EQKPQIPTKLGDHINLCS-----DSISKRKILPIEPTSKPQSPIQLLKSAPKFPVFIFKK 120

Query: 121 QKSMAKNRAAAGSEPARGEQEEEENRIPSSKLARDNSTRRIGGLFPADPS----PAPARR 180
           Q+S+AKNRA AG+E      EEE +R+ SSKL+RDNSTRRIGG+FP DP     PAP RR
Sbjct: 121 QRSVAKNRAPAGTE------EEEGSRVLSSKLSRDNSTRRIGGIFPLDPREPPVPAPTRR 180

Query: 181 FSKETVQKYLKLIKPKALKKP---AGD--DPPPDSPMREKQENSFPAGIRLVCKHLGKSK 240
           FSKE VQKYLKLIKPK L+KP    GD      DSP+REK EN  PAGIRLVC+HLGKSK
Sbjct: 181 FSKEAVQKYLKLIKPKVLRKPTYSGGDFSSAAVDSPVREKHENGLPAGIRLVCRHLGKSK 240

Query: 241 SASSS-------ATGAPPPNRRDDSLLQQHDGIQSAILHCKRSFNSSTGK 271
           SAS++       +   PPPNRRDDSLLQQHDGIQSAILHCKRSFN+S  K
Sbjct: 241 SASAAGGLAAAVSAVVPPPNRRDDSLLQQHDGIQSAILHCKRSFNASAEK 265

[Close]

BLAST of MS006250 vs. NCBI nr
Match: KAA0042587.1 (putative membrane-associated kinase regulator 5 [Cucumis melo var. makuwa] >TYK05990.1 putative membrane-associated kinase regulator 5 [Cucumis melo var. makuwa])

HSP 1 Score: 317.8 bits (813), Expect = 9.3e-83
Identity = 186/294 (63.27%), Postives = 214/294 (72.79%), Query Frame = 0


Query: 1   MDSVAFLKFWKIAACPITTNNAIFSTADEQEQQKEEEEEEEDSFIDLELLSVSAAE---- 60
           MDSVAFLKF+K +      +       +E+E+++EEEEEEEDSF D+E  SVSA +    
Sbjct: 1   MDSVAFLKFFKHSG----ADELQKQEEEEEEEEEEEEEEEEDSFFDIEFFSVSAGKIQSP 60

Query: 61  AQRPQIPTKLGDHINLCSRDLQTDSISKRKILPIDQSPKPQSPIQLLKSAPKFPVFIFKK 120
            QRPQIPTKLGDHINLCS     DSISKRKILPI+ + KPQSPIQLLKSAPKFPVFIFKK
Sbjct: 61  EQRPQIPTKLGDHINLCS-----DSISKRKILPIEPTSKPQSPIQLLKSAPKFPVFIFKK 120

Query: 121 QKSMAKNRAAAGSEPARGEQEEEENRIPSSKLARDNSTRRIGGLFPADPS----PAPARR 180
           Q+SMAKN+A          QE +++ +PSSKL RDNSTRR GG+FP+DP     PAP RR
Sbjct: 121 QRSMAKNKAPTA-------QEGDQSGVPSSKLTRDNSTRRFGGIFPSDPCEPPVPAPTRR 180

Query: 181 FSKETVQKYLKLIKPKALKKP--AGDD------PPPDSPMREKQENSFPAGIRLVCKHLG 240
           FSKE V+KY KLIKPK L+KP   G D         DSP+REK EN  PAGIRLVC+HLG
Sbjct: 181 FSKEAVRKYFKLIKPKVLRKPTDTGSDLSSSSAAAVDSPVREKHENGLPAGIRLVCRHLG 240

Query: 241 KSKSASS----SATGAPPPNRRDDSLLQQHDGIQSAILHCKRSFNSSTGKSTKL 275
           KSKSAS+    +A   PPPNRRDDSLLQQHDGIQSAILHCKRSFN+S+ K   L
Sbjct: 241 KSKSASAAGGLAAAVVPPPNRRDDSLLQQHDGIQSAILHCKRSFNASSEKPLSL 278

[Close]

BLAST of MS006250 vs. NCBI nr
Match: XP_004145905.2 (probable membrane-associated kinase regulator 5 [Cucumis sativus] >KGN49913.1 hypothetical protein Csa_000048 [Cucumis sativus])

HSP 1 Score: 309.7 bits (792), Expect = 2.5e-80
Identity = 189/291 (64.95%), Postives = 210/291 (72.16%), Query Frame = 0


Query: 1   MDSVAFLKFWKIAACPITTNNAIFSTADEQEQQKEEEEEE----EDSFIDLELLSVSAAE 60
           MDSVAFLKF+K             S ADE ++Q+EEEEEE    EDSF D+E  SVSA +
Sbjct: 1   MDSVAFLKFFK------------HSGADELQKQEEEEEEEEEEGEDSFFDIEFFSVSAGK 60

Query: 61  ----AQRPQIPTKLGDHINLCSRDLQTDSISKRKILPIDQSPKPQSPIQLLKSAPKFPVF 120
                QRPQIPTKLGDHINLCS     DSISKRKILPI+ + KPQSPIQLLKSAPKFPVF
Sbjct: 61  IQSPEQRPQIPTKLGDHINLCS-----DSISKRKILPIEPTSKPQSPIQLLKSAPKFPVF 120

Query: 121 IFKKQKSMAKNRAAAGSEPARGEQEEEENRIPSSKLARDNSTRRIGGLFPADPS----PA 180
           IFKKQ+SMAKNR   G       QE  E+R+ +SKL RDNSTRR GG+FP DP     PA
Sbjct: 121 IFKKQRSMAKNRPPTG-------QEGGESRVLNSKLTRDNSTRRFGGIFPPDPCEPPVPA 180

Query: 181 PARRFSKETVQKYLKLIKPKALKKP--AGDD---PPPDSPMREKQENSFPAGIRLVCKHL 240
           P RRFSKE VQKYLKLIKPK L+KP  +G D      DSP+REK EN  PAGIRLVC+HL
Sbjct: 181 PTRRFSKEVVQKYLKLIKPKVLRKPTDSGSDLSSAAVDSPVREKHENGLPAGIRLVCRHL 240

Query: 241 GKSKSASS----SATGAPPPNRRDDSLLQQHDGIQSAILHCKRSFNSSTGK 271
           GKSKSAS+    +A   P  NRRDDSLLQQHDGIQSAILHCKRSFN+S+ K
Sbjct: 241 GKSKSASAAGGLAAAVIPQTNRRDDSLLQQHDGIQSAILHCKRSFNASSEK 267

[Close]

BLAST of MS006250 vs. NCBI nr
Match: XP_022973059.1 (probable membrane-associated kinase regulator 5 [Cucurbita maxima])

HSP 1 Score: 307.8 bits (787), Expect = 9.6e-80
Identity = 188/292 (64.38%), Postives = 209/292 (71.58%), Query Frame = 0


Query: 1   MDSVAFLKFWKIAACPITTNNAIFSTAD---EQEQQKEEEEEEEDSFIDLELLSVSAAE- 60
           MDSVA+LKF+K            FS AD   +QEQ +EEEEEEEDSF DLELLSVSA + 
Sbjct: 1   MDSVAYLKFFK------------FSGADGLPKQEQDEEEEEEEEDSFFDLELLSVSAGKI 60

Query: 61  ---AQRPQIPTKLGDHINLCSRDLQTDSISKRKILPIDQSPKPQSPIQLLKSAPKFPVFI 120
               QRPQIPTKLG+H+NLCS     DSISKRKILPI+ + KPQSPIQ LKSAPKF  FI
Sbjct: 61  QGPEQRPQIPTKLGEHMNLCS-----DSISKRKILPIEPTSKPQSPIQFLKSAPKFTDFI 120

Query: 121 FKKQKSMAKNRAAAGSEPARGEQEEEENRIPSSKLARDNSTRRIGGLFPAD----PSPAP 180
           FKKQ+SMAKNRA AG        E+EE RI SSK  RDNSTRRI G+FP D    P+P+P
Sbjct: 121 FKKQRSMAKNRAPAG-------PEQEETRILSSKFTRDNSTRRIRGIFPPDPYGVPAPSP 180

Query: 181 ARRFSKETVQKYLKLIKPKALKKPAG-----DDPPPDSPMREKQENSFPAGIRLVCKHLG 240
           A RFSKE VQKYLKLIKPK LKKP            DSP+REK EN  P GIRLVCKHLG
Sbjct: 181 AWRFSKEAVQKYLKLIKPKLLKKPTDSGGELSSAAVDSPVREKLENGLPTGIRLVCKHLG 240

Query: 241 KSKSASSSA------TGAPPPNRRDDSLLQQHDGIQSAILHCKRSFNSSTGK 271
           +SKSAS+++        APPPNRRDDSLLQ HDGI+SAILHCKRS N+ST K
Sbjct: 241 RSKSASAASRLAAAVAVAPPPNRRDDSLLQLHDGIESAILHCKRSLNASTEK 268

[Close]

BLAST of MS006250 vs. ExPASy Swiss-Prot
Match: Q9FLX4 (Membrane-associated kinase regulator 5 OS=Arabidopsis thaliana OX=3702 GN=MAKR5 PE=2 SV=1)

HSP 1 Score: 99.0 bits (245), Expect = 8.9e-20
Identity = 98/291 (33.68%), Postives = 141/291 (48.45%), Query Frame = 0


Query: 1   MDSVAFLKFWKIAACPITTNNAIFSTADEQEQQKEEEE------EEEDSFIDLELLSVSA 60
           M+++ F+KFW      I     I  +    +     E+      E EDSF +L+   +S 
Sbjct: 1   MEALTFMKFWLTNNTTIKPRREIRISESAVDSTTGSEDPDLDLCEGEDSFFELK---ISL 60

Query: 61  AEAQRPQIPTKLGDHINLCSRDLQTDSIS-KRKILP-IDQSPKP-QSPIQLLKSAPKFPV 120
           ++ + P+   +L       +    T S+S K K+LP +D S KP QSP  LLKS  KF  
Sbjct: 61  SDFKTPKEKQRLE-----TTTTTTTYSVSNKSKVLPFVDISSKPQQSPTTLLKSGQKFRA 120

Query: 121 FIFKKQKSMAKNRAAAGSEPARGEQEEEENRIPSSKLARDNSTRRIGGLFPADPSPAPAR 180
           F FKK +                E+++EEN   S  +            F    S A ++
Sbjct: 121 FSFKKSEKSTTT-----------EKKKEENNRTSLNVRFRVDDETTTTSFRKTASIARSQ 180

Query: 181 R-----FSKETVQKYLKLIKP---KALKKPAGD--DPPPDSP-MREKQENSFPAGIRLVC 240
           +     F     +++  LIKP   K+ KK +      P  SP  REKQ ++ P+G+R V 
Sbjct: 181 QTDDTMFDDSVSKRFFSLIKPLYTKSTKKQSSSTITSPTSSPATREKQRSNIPSGMRSVR 240

Query: 241 KHLGKSKSASSSATGAPPPNRRDDSLLQQHDGIQSAILHCKRSFNSSTGKS 272
           + LGKS+SAS++  G  P NR D+SL  Q DGIQSAILHCK+SF+ S   S
Sbjct: 241 RQLGKSRSASAAIGGMSPANRIDESLQVQQDGIQSAILHCKKSFHGSRESS 272

[Close]

BLAST of MS006250 vs. ExPASy Swiss-Prot
Match: Q9SH58 (Probable membrane-associated kinase regulator 2 OS=Arabidopsis thaliana OX=3702 GN=MAKR2 PE=2 SV=1)

HSP 1 Score: 75.5 bits (184), Expect = 1.1e-12
Identity = 70/219 (31.96%), Postives = 102/219 (46.58%), Query Frame = 0


Query: 89  PIDQSPKPQ--SPIQLLKSAPKFPVFIFKKQKSMAKNRAAAGSEPARGEQEEEENRIPSS 148
           P+  SP+ Q  S + +     + P+     +++ +KN +++ S  +            SS
Sbjct: 197 PLSLSPESQQKSTVTVNLKVEEVPIISLFSRENSSKNSSSSSSSSSSS----------SS 256

Query: 149 KLARDNSTRRIGGLFPADPSPAPARRFSKETVQKYLKLIKPKALK---------KPAGDD 208
            L + N    +          +  +RF    +QKYLK +KP  ++         + +G  
Sbjct: 257 SLKKQNGNESV---------VSDEKRF--VMMQKYLKKVKPLYIRVSRRYGEKLRHSGQL 316

Query: 209 PPP----------DSPMREKQEN------SFPAGIRLVCKHLGKSKSASSSA-------- 268
             P          DSP+  K  N      + PAG+++V KHLGKS+S+SS+A        
Sbjct: 317 TAPSSARSTAEKADSPVATKTNNKPGNNINIPAGLKVVRKHLGKSRSSSSTATTPSSTTA 376

Query: 269 --TGAPPPNRRDDSLLQQHDGIQSAILHCKRSFNSSTGK 271
             T      RRDDSLLQQ D IQSAILHCKRSFNSS  K
Sbjct: 377 TVTTQSESRRRDDSLLQQQDSIQSAILHCKRSFNSSRDK 394

[Close]

BLAST of MS006250 vs. ExPASy TrEMBL
Match: A0A1S3AUW4 (probable membrane-associated kinase regulator 5 OS=Cucumis melo OX=3656 GN=LOC103482925 PE=4 SV=1)

HSP 1 Score: 319.7 bits (818), Expect = 1.2e-83
Identity = 188/294 (63.95%), Postives = 215/294 (73.13%), Query Frame = 0


Query: 1   MDSVAFLKFWKIAACPITTNNAIFSTADEQEQQKEEEEEEEDSFIDLELLSVSAAE---- 60
           MDSVAFLKF+K        + A      E+E+++EEEEEEEDSF D+E  SVSA +    
Sbjct: 1   MDSVAFLKFFK-------HSGADELQKQEEEEEEEEEEEEEDSFFDIEFFSVSAGKIQSP 60

Query: 61  AQRPQIPTKLGDHINLCSRDLQTDSISKRKILPIDQSPKPQSPIQLLKSAPKFPVFIFKK 120
            QRPQIPTKLGDHINLCS     DSISKRKILPI+ + KPQSPIQLLKSAPKFPVFIFKK
Sbjct: 61  EQRPQIPTKLGDHINLCS-----DSISKRKILPIEPTSKPQSPIQLLKSAPKFPVFIFKK 120

Query: 121 QKSMAKNRAAAGSEPARGEQEEEENRIPSSKLARDNSTRRIGGLFPADPS----PAPARR 180
           Q+SMAKN+A  G       QE +++ +PSSKL RDNSTRR GG+FP+DP     PAP RR
Sbjct: 121 QRSMAKNKAPTG-------QEGDQSGVPSSKLTRDNSTRRFGGIFPSDPCEPPVPAPTRR 180

Query: 181 FSKETVQKYLKLIKPKALKKP--AGDD------PPPDSPMREKQENSFPAGIRLVCKHLG 240
           FSKE V+KY KLIKPK L+KP  +G D         DSP+REK EN  PAGIRLVC+HLG
Sbjct: 181 FSKEAVRKYFKLIKPKVLRKPTDSGSDLSSSSAAAVDSPVREKHENGLPAGIRLVCRHLG 240

Query: 241 KSKSASS----SATGAPPPNRRDDSLLQQHDGIQSAILHCKRSFNSSTGKSTKL 275
           KSKSAS+    +A   PPPNRRDDSLLQQHDGIQSAILHCKRSFN+S+ K   L
Sbjct: 241 KSKSASAAGGLAAAVVPPPNRRDDSLLQQHDGIQSAILHCKRSFNASSEKPLSL 275

[Close]

BLAST of MS006250 vs. ExPASy TrEMBL
Match: A0A5D3C3T3 (Putative membrane-associated kinase regulator 5 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold376G00860 PE=4 SV=1)

HSP 1 Score: 317.8 bits (813), Expect = 4.5e-83
Identity = 186/294 (63.27%), Postives = 214/294 (72.79%), Query Frame = 0


Query: 1   MDSVAFLKFWKIAACPITTNNAIFSTADEQEQQKEEEEEEEDSFIDLELLSVSAAE---- 60
           MDSVAFLKF+K +      +       +E+E+++EEEEEEEDSF D+E  SVSA +    
Sbjct: 1   MDSVAFLKFFKHSG----ADELQKQEEEEEEEEEEEEEEEEDSFFDIEFFSVSAGKIQSP 60

Query: 61  AQRPQIPTKLGDHINLCSRDLQTDSISKRKILPIDQSPKPQSPIQLLKSAPKFPVFIFKK 120
            QRPQIPTKLGDHINLCS     DSISKRKILPI+ + KPQSPIQLLKSAPKFPVFIFKK
Sbjct: 61  EQRPQIPTKLGDHINLCS-----DSISKRKILPIEPTSKPQSPIQLLKSAPKFPVFIFKK 120

Query: 121 QKSMAKNRAAAGSEPARGEQEEEENRIPSSKLARDNSTRRIGGLFPADPS----PAPARR 180
           Q+SMAKN+A          QE +++ +PSSKL RDNSTRR GG+FP+DP     PAP RR
Sbjct: 121 QRSMAKNKAPTA-------QEGDQSGVPSSKLTRDNSTRRFGGIFPSDPCEPPVPAPTRR 180

Query: 181 FSKETVQKYLKLIKPKALKKP--AGDD------PPPDSPMREKQENSFPAGIRLVCKHLG 240
           FSKE V+KY KLIKPK L+KP   G D         DSP+REK EN  PAGIRLVC+HLG
Sbjct: 181 FSKEAVRKYFKLIKPKVLRKPTDTGSDLSSSSAAAVDSPVREKHENGLPAGIRLVCRHLG 240

Query: 241 KSKSASS----SATGAPPPNRRDDSLLQQHDGIQSAILHCKRSFNSSTGKSTKL 275
           KSKSAS+    +A   PPPNRRDDSLLQQHDGIQSAILHCKRSFN+S+ K   L
Sbjct: 241 KSKSASAAGGLAAAVVPPPNRRDDSLLQQHDGIQSAILHCKRSFNASSEKPLSL 278

[Close]

BLAST of MS006250 vs. ExPASy TrEMBL
Match: A0A0A0KK03 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G139900 PE=4 SV=1)

HSP 1 Score: 309.7 bits (792), Expect = 1.2e-80
Identity = 189/291 (64.95%), Postives = 210/291 (72.16%), Query Frame = 0


Query: 1   MDSVAFLKFWKIAACPITTNNAIFSTADEQEQQKEEEEEE----EDSFIDLELLSVSAAE 60
           MDSVAFLKF+K             S ADE ++Q+EEEEEE    EDSF D+E  SVSA +
Sbjct: 1   MDSVAFLKFFK------------HSGADELQKQEEEEEEEEEEGEDSFFDIEFFSVSAGK 60

Query: 61  ----AQRPQIPTKLGDHINLCSRDLQTDSISKRKILPIDQSPKPQSPIQLLKSAPKFPVF 120
                QRPQIPTKLGDHINLCS     DSISKRKILPI+ + KPQSPIQLLKSAPKFPVF
Sbjct: 61  IQSPEQRPQIPTKLGDHINLCS-----DSISKRKILPIEPTSKPQSPIQLLKSAPKFPVF 120

Query: 121 IFKKQKSMAKNRAAAGSEPARGEQEEEENRIPSSKLARDNSTRRIGGLFPADPS----PA 180
           IFKKQ+SMAKNR   G       QE  E+R+ +SKL RDNSTRR GG+FP DP     PA
Sbjct: 121 IFKKQRSMAKNRPPTG-------QEGGESRVLNSKLTRDNSTRRFGGIFPPDPCEPPVPA 180

Query: 181 PARRFSKETVQKYLKLIKPKALKKP--AGDD---PPPDSPMREKQENSFPAGIRLVCKHL 240
           P RRFSKE VQKYLKLIKPK L+KP  +G D      DSP+REK EN  PAGIRLVC+HL
Sbjct: 181 PTRRFSKEVVQKYLKLIKPKVLRKPTDSGSDLSSAAVDSPVREKHENGLPAGIRLVCRHL 240

Query: 241 GKSKSASS----SATGAPPPNRRDDSLLQQHDGIQSAILHCKRSFNSSTGK 271
           GKSKSAS+    +A   P  NRRDDSLLQQHDGIQSAILHCKRSFN+S+ K
Sbjct: 241 GKSKSASAAGGLAAAVIPQTNRRDDSLLQQHDGIQSAILHCKRSFNASSEK 267

[Close]

BLAST of MS006250 vs. ExPASy TrEMBL
Match: A0A6J1IDF3 (probable membrane-associated kinase regulator 5 OS=Cucurbita maxima OX=3661 GN=LOC111471559 PE=4 SV=1)

HSP 1 Score: 307.8 bits (787), Expect = 4.7e-80
Identity = 188/292 (64.38%), Postives = 209/292 (71.58%), Query Frame = 0


Query: 1   MDSVAFLKFWKIAACPITTNNAIFSTAD---EQEQQKEEEEEEEDSFIDLELLSVSAAE- 60
           MDSVA+LKF+K            FS AD   +QEQ +EEEEEEEDSF DLELLSVSA + 
Sbjct: 1   MDSVAYLKFFK------------FSGADGLPKQEQDEEEEEEEEDSFFDLELLSVSAGKI 60

Query: 61  ---AQRPQIPTKLGDHINLCSRDLQTDSISKRKILPIDQSPKPQSPIQLLKSAPKFPVFI 120
               QRPQIPTKLG+H+NLCS     DSISKRKILPI+ + KPQSPIQ LKSAPKF  FI
Sbjct: 61  QGPEQRPQIPTKLGEHMNLCS-----DSISKRKILPIEPTSKPQSPIQFLKSAPKFTDFI 120

Query: 121 FKKQKSMAKNRAAAGSEPARGEQEEEENRIPSSKLARDNSTRRIGGLFPAD----PSPAP 180
           FKKQ+SMAKNRA AG        E+EE RI SSK  RDNSTRRI G+FP D    P+P+P
Sbjct: 121 FKKQRSMAKNRAPAG-------PEQEETRILSSKFTRDNSTRRIRGIFPPDPYGVPAPSP 180

Query: 181 ARRFSKETVQKYLKLIKPKALKKPAG-----DDPPPDSPMREKQENSFPAGIRLVCKHLG 240
           A RFSKE VQKYLKLIKPK LKKP            DSP+REK EN  P GIRLVCKHLG
Sbjct: 181 AWRFSKEAVQKYLKLIKPKLLKKPTDSGGELSSAAVDSPVREKLENGLPTGIRLVCKHLG 240

Query: 241 KSKSASSSA------TGAPPPNRRDDSLLQQHDGIQSAILHCKRSFNSSTGK 271
           +SKSAS+++        APPPNRRDDSLLQ HDGI+SAILHCKRS N+ST K
Sbjct: 241 RSKSASAASRLAAAVAVAPPPNRRDDSLLQLHDGIESAILHCKRSLNASTEK 268

[Close]

BLAST of MS006250 vs. ExPASy TrEMBL
Match: A0A6J1H2I5 (probable membrane-associated kinase regulator 5 OS=Cucurbita moschata OX=3662 GN=LOC111459782 PE=4 SV=1)

HSP 1 Score: 305.4 bits (781), Expect = 2.3e-79
Identity = 184/285 (64.56%), Postives = 207/285 (72.63%), Query Frame = 0


Query: 1   MDSVAFLKFWKIAACPITTNNAIFSTADEQEQQKEEEEEEEDSFIDLELLSVSAAE---- 60
           MDS+AF + +K+            S ADEQ++++++ EEEEDSF DLE LSVSA +    
Sbjct: 31  MDSLAFRRLFKL------------SVADEQQKREQDGEEEEDSFFDLEFLSVSAGDIHSP 90

Query: 61  AQRPQIPTKLGDHINLCSRDLQTDSISKRKILPIDQSPKPQSPIQLLKSAPKFPVFIFKK 120
            QRPQIPTKLG+HINLCS     DSISKRKILPI+ + KP SPIQLLKSAPKFPV IF K
Sbjct: 91  EQRPQIPTKLGEHINLCS-----DSISKRKILPIEPTSKPHSPIQLLKSAPKFPVSIFNK 150

Query: 121 QKSMAKNRAAAGSEPARGEQEEEENRIPSSKLARDNSTRRIGGLFPADPSPAP---ARRF 180
           Q+SMAKNRA  G+       E+ E R+ SS L+RDNSTRRIGG+FP DP  AP   ARRF
Sbjct: 151 QRSMAKNRAPGGT-------EQVERRVLSSNLSRDNSTRRIGGVFPVDPCEAPATTARRF 210

Query: 181 SKETVQKYLKLIKPKALKKPA----GDDPPPDSPMREKQENSFPAGIRLVCKHLGKSKSA 240
           SKE VQKYL+LIKPK LKKP           DSP REK +N  PAGIRLVCKHLGKSKSA
Sbjct: 211 SKEAVQKYLRLIKPKVLKKPTDYSDNLSTTVDSPAREKHDNGLPAGIRLVCKHLGKSKSA 270

Query: 241 SSSATG----APPPNRRDDSLLQQHDGIQSAILHCKRSFNSSTGK 271
             SATG    APP NRRDDSLLQQHDGIQSAILHCKRSFN+ST K
Sbjct: 271 --SATGGLAVAPPLNRRDDSLLQQHDGIQSAILHCKRSFNASTEK 289

[Close]

BLAST of MS006250 vs. TAIR 10
Match: AT5G52870.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G64080.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). )

HSP 1 Score: 99.0 bits (245), Expect = 6.3e-21
Identity = 98/291 (33.68%), Postives = 141/291 (48.45%), Query Frame = 0


Query: 1   MDSVAFLKFWKIAACPITTNNAIFSTADEQEQQKEEEE------EEEDSFIDLELLSVSA 60
           M+++ F+KFW      I     I  +    +     E+      E EDSF +L+   +S 
Sbjct: 1   MEALTFMKFWLTNNTTIKPRREIRISESAVDSTTGSEDPDLDLCEGEDSFFELK---ISL 60

Query: 61  AEAQRPQIPTKLGDHINLCSRDLQTDSIS-KRKILP-IDQSPKP-QSPIQLLKSAPKFPV 120
           ++ + P+   +L       +    T S+S K K+LP +D S KP QSP  LLKS  KF  
Sbjct: 61  SDFKTPKEKQRLE-----TTTTTTTYSVSNKSKVLPFVDISSKPQQSPTTLLKSGQKFRA 120

Query: 121 FIFKKQKSMAKNRAAAGSEPARGEQEEEENRIPSSKLARDNSTRRIGGLFPADPSPAPAR 180
           F FKK +                E+++EEN   S  +            F    S A ++
Sbjct: 121 FSFKKSEKSTTT-----------EKKKEENNRTSLNVRFRVDDETTTTSFRKTASIARSQ 180

Query: 181 R-----FSKETVQKYLKLIKP---KALKKPAGD--DPPPDSP-MREKQENSFPAGIRLVC 240
           +     F     +++  LIKP   K+ KK +      P  SP  REKQ ++ P+G+R V 
Sbjct: 181 QTDDTMFDDSVSKRFFSLIKPLYTKSTKKQSSSTITSPTSSPATREKQRSNIPSGMRSVR 240

Query: 241 KHLGKSKSASSSATGAPPPNRRDDSLLQQHDGIQSAILHCKRSFNSSTGKS 272
           + LGKS+SAS++  G  P NR D+SL  Q DGIQSAILHCK+SF+ S   S
Sbjct: 241 RQLGKSRSASAAIGGMSPANRIDESLQVQQDGIQSAILHCKKSFHGSRESS 272

[Close]

BLAST of MS006250 vs. TAIR 10
Match: AT1G64080.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: plasma membrane; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G52870.1); Has 1546 Blast hits to 738 proteins in 176 species: Archae - 0; Bacteria - 143; Metazoa - 278; Fungi - 131; Plants - 95; Viruses - 4; Other Eukaryotes - 895 (source: NCBI BLink). )

HSP 1 Score: 75.5 bits (184), Expect = 7.5e-14
Identity = 70/219 (31.96%), Postives = 102/219 (46.58%), Query Frame = 0


Query: 89  PIDQSPKPQ--SPIQLLKSAPKFPVFIFKKQKSMAKNRAAAGSEPARGEQEEEENRIPSS 148
           P+  SP+ Q  S + +     + P+     +++ +KN +++ S  +            SS
Sbjct: 197 PLSLSPESQQKSTVTVNLKVEEVPIISLFSRENSSKNSSSSSSSSSSS----------SS 256

Query: 149 KLARDNSTRRIGGLFPADPSPAPARRFSKETVQKYLKLIKPKALK---------KPAGDD 208
            L + N    +          +  +RF    +QKYLK +KP  ++         + +G  
Sbjct: 257 SLKKQNGNESV---------VSDEKRF--VMMQKYLKKVKPLYIRVSRRYGEKLRHSGQL 316

Query: 209 PPP----------DSPMREKQEN------SFPAGIRLVCKHLGKSKSASSSA-------- 268
             P          DSP+  K  N      + PAG+++V KHLGKS+S+SS+A        
Sbjct: 317 TAPSSARSTAEKADSPVATKTNNKPGNNINIPAGLKVVRKHLGKSRSSSSTATTPSSTTA 376

Query: 269 --TGAPPPNRRDDSLLQQHDGIQSAILHCKRSFNSSTGK 271
             T      RRDDSLLQQ D IQSAILHCKRSFNSS  K
Sbjct: 377 TVTTQSESRRRDDSLLQQQDSIQSAILHCKRSFNSSRDK 394

The following BLAST results are available for this feature:

BLAST of MS006250 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008437543.1	2.5e-83	63.95	PREDICTED: probable membrane-associated kinase regulator 5 [Cucumis melo]	[more]
XP_038906856.1	2.5e-83	65.52	probable membrane-associated kinase regulator 5 [Benincasa hispida]	[more]
KAA0042587.1	9.3e-83	63.27	putative membrane-associated kinase regulator 5 [Cucumis melo var. makuwa] >TYK0...	[more]
XP_004145905.2	2.5e-80	64.95	probable membrane-associated kinase regulator 5 [Cucumis sativus] >KGN49913.1 hy...	[more]
XP_022973059.1	9.6e-80	64.38	probable membrane-associated kinase regulator 5 [Cucurbita maxima]	[more]

BLAST of MS006250 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FLX4	8.9e-20	33.68	Membrane-associated kinase regulator 5 OS=Arabidopsis thaliana OX=3702 GN=MAKR5 ...	[more]
Q9SH58	1.1e-12	31.96	Probable membrane-associated kinase regulator 2 OS=Arabidopsis thaliana OX=3702 ...	[more]

BLAST of MS006250 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3AUW4	1.2e-83	63.95	probable membrane-associated kinase regulator 5 OS=Cucumis melo OX=3656 GN=LOC10...	[more]
A0A5D3C3T3	4.5e-83	63.27	Putative membrane-associated kinase regulator 5 OS=Cucumis melo var. makuwa OX=1...	[more]
A0A0A0KK03	1.2e-80	64.95	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G139900 PE=4 SV=1	[more]
A0A6J1IDF3	4.7e-80	64.38	probable membrane-associated kinase regulator 5 OS=Cucurbita maxima OX=3661 GN=L...	[more]
A0A6J1H2I5	2.3e-79	64.56	probable membrane-associated kinase regulator 5 OS=Cucurbita moschata OX=3662 GN...	[more]

BLAST of MS006250 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G52870.1	6.3e-21	33.68	unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA...	[more]
AT1G64080.1	7.5e-14	31.96	unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic...	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 20..47
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 189..204
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 122..252
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 131..146
None	No IPR available	PANTHER	PTHR33929:SF4	MEMBRANE-ASSOCIATED KINASE REGULATOR 5-RELATED	coord: 1..268
IPR039619	Membrane-associated kinase regulator 2/5	PANTHER	PTHR33929	MEMBRANE-ASSOCIATED KINASE REGULATOR 2-RELATED	coord: 1..268

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS006250.1	MS006250.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0005886	plasma membrane