MS006249 (gene) Bitter gourd (TR) v1

Overview


Name	MS006249
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	F-box protein
Location	scaffold96: 1563604 .. 1565644 (+)
RNA-Seq Expression	MS006249
Synteny	MS006249

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCGGATCCATTAGAGAGGTACCGGAAGCTTGCCCTAAGAGATTCTCTGGCGAAGAGTTATCGCTACCCATTAGCCTGTAAGGAGCTAAGCTTCATTATCAGAGCTGCTTTCAATAAACTTCCCAAGAATCTCCAATCCCTCATCTACCAAGATATCATCACCGCCTTTCATCTCCTTCCCGAGTAAGTTTTTGTGTTTCTTCCCTTCTTTTCATATTCAATTTTAGAATTCATATGTTGATTACCCATATACTTTGTCTCCAGTTCGTCGGTTTCAATTCTCCATATTTGGATCAAATTAGGAATTGTTATGAACCCTTTGAATGTTTGCTTCTTAAATCCCTTGCCTGTGCCTGGTTGATTTTATGAAACAGTTTAGTTCTTTTATGGGTTGGATTCAACATTCAATTGAATGTCTTAATGTTCCACAATCCAAGCATTCATTGTCCATGTTAATTGAAGACTACTATAGATTTATCTATCATTCATCAATTTGTTAAAGAGGATACACAAAGTAGTCGATCTATCGTTTATATGAGAATGCGGTTGTCTACGCCGTTGATTCCATTTTATGGCATTGAAACTAAGCAAGATTACTATTAATAGTTTTTGTTCCATCCTCTCTCTAATTTGCCTACACTCCTTTGATGCTTATTTGAATACGGGAAGCTTCATATGAAATGTTCTGATTAAAAGAATGCAGACGAGCTCTGCTGTTTCGGCTGCACATCTCCTTGCTCAGGGTGTTGAAGCTGCTTGGCCTAAGAAAAAGAGAAATTCAGCAATCGTAGAATTCAAGAAAGCAATGGTTGCTCATAAGAGGCGTAGTAAAGCACATCAGGAAGAAAAAGGTGAATCGCTTGTAAGAATGATTTAACTTTTCCCTTCTTTCTGGTATATTTTGCTATTCATAAATCAAATCGAACAAGATTTAAGTTTGGATAAATTACACATTTGGTTCATGAACTTTTATAGCTATGTCTAATAGGTCACTAAACTTTTAATATTGTGTCTAATAGGTCTCCAAATTTTAAAAAGTGTCTAATAGATTCTTGAACTTTCAATTTTATGTCAATGAGTATATTAAACACTTTTTAAATTCATGAGCTTATTAGGGAAAAAAAATCATAAATAAAATGTTTGAGAACTTATTAGACCCTTTTCAAAGGTCTTACCTGATGGATACTTTTTATAATTTAGAGACCTACTAGATACAAATTTGAGAGTTAAGAGTCCACACTTGTATTTTAACTTTCAAATTTTCATCTTTATGTTTGGCAACACAATTTGATTTCTTTCTCATAAATATTTTTAGGTTCCGCTCAACTGCCTCAAGATGTACTCCTCCACATCTTCCGCTTTCTAGATATCGAATCTTTAGTCTCTGCTGGACTAGTCTGCCGGTAATTTTCTGTCTAAGACCACATGACATGAAATATCCTTGTCTAATCAACAAAGTGTGTGCTTAGCTTAAAGCTCTACTATTCAACTATGAGTTCTAAGCACTACTCTCTGTATGTTGTTAGACACTTAGTTTATTAGCCTTATACGGCGACCATTTCCTTTTAAGATAACGGTTGTTATATACTCAATGTGAAGGTCATGGAATGTAGCAGCAGATGATGAATTTCTATGGCAGTTGCAGTATACAACATTTTTCGGTTCTGATGACAGTGCTAAACTCATTAACAACAAAGGCGGTGTGGACGACTTGGCTAGCACACCTCCTCATGTTGATTGGAAAGAGGCATTTAAGAGAGCATATATAGGTGGATTTTCCCTGCCATAGCTCTCTTTCCTAATAAAACAAAATTTTCAGTAAGATAGTTTTTGAATTTCATAATAACATCTAATTCTTGTTGCTACCATGGTCCATGGCATTTGGAATTTTAGGTAATTCTTTGAGAAGAAACACATATGGAATGGGATACTGCAAACACTGTAACACAATAGTTTTCGTCAGCACCTTGAGATGTCCCGTCGATCATGGAAAAAGTAAAAATAAGCAAATCAAGCCTCTCTCAATCAACCAGGTACTT

mRNA sequence

ATGTCGGATCCATTAGAGAGGTACCGGAAGCTTGCCCTAAGAGATTCTCTGGCGAAGAGTTATCGCTACCCATTAGCCTGTAAGGAGCTAAGCTTCATTATCAGAGCTGCTTTCAATAAACTTCCCAAGAATCTCCAATCCCTCATCTACCAAGATATCATCACCGCCTTTCATCTCCTTCCCGAAATGCAGACGAGCTCTGCTGTTTCGGCTGCACATCTCCTTGCTCAGGGTGTTGAAGCTGCTTGGCCTAAGAAAAAGAGAAATTCAGCAATCGTAGAATTCAAGAAAGCAATGGTTGCTCATAAGAGGCGTAGTAAAGCACATCAGGAAGAAAAAGGTTCCGCTCAACTGCCTCAAGATGTACTCCTCCACATCTTCCGCTTTCTAGATATCGAATCTTTAGTCTCTGCTGGACTAGTCTGCCGGTCATGGAATGTAGCAGCAGATGATGAATTTCTATGGCAGTTGCAGTATACAACATTTTTCGGTTCTGATGACAGTGCTAAACTCATTAACAACAAAGGCGGTGTGGACGACTTGGCTAGCACACCTCCTCATGTTGATTGGAAAGAGGCATTTAAGAGAGCATATATAGGTAATTCTTTGAGAAGAAACACATATGGAATGGGATACTGCAAACACTGTAACACAATAGTTTTCGTCAGCACCTTGAGATGTCCCGTCGATCATGGAAAAAGTAAAAATAAGCAAATCAAGCCTCTCTCAATCAACCAGGTACTT

Coding sequence (CDS)

ATGTCGGATCCATTAGAGAGGTACCGGAAGCTTGCCCTAAGAGATTCTCTGGCGAAGAGTTATCGCTACCCATTAGCCTGTAAGGAGCTAAGCTTCATTATCAGAGCTGCTTTCAATAAACTTCCCAAGAATCTCCAATCCCTCATCTACCAAGATATCATCACCGCCTTTCATCTCCTTCCCGAAATGCAGACGAGCTCTGCTGTTTCGGCTGCACATCTCCTTGCTCAGGGTGTTGAAGCTGCTTGGCCTAAGAAAAAGAGAAATTCAGCAATCGTAGAATTCAAGAAAGCAATGGTTGCTCATAAGAGGCGTAGTAAAGCACATCAGGAAGAAAAAGGTTCCGCTCAACTGCCTCAAGATGTACTCCTCCACATCTTCCGCTTTCTAGATATCGAATCTTTAGTCTCTGCTGGACTAGTCTGCCGGTCATGGAATGTAGCAGCAGATGATGAATTTCTATGGCAGTTGCAGTATACAACATTTTTCGGTTCTGATGACAGTGCTAAACTCATTAACAACAAAGGCGGTGTGGACGACTTGGCTAGCACACCTCCTCATGTTGATTGGAAAGAGGCATTTAAGAGAGCATATATAGGTAATTCTTTGAGAAGAAACACATATGGAATGGGATACTGCAAACACTGTAACACAATAGTTTTCGTCAGCACCTTGAGATGTCCCGTCGATCATGGAAAAAGTAAAAATAAGCAAATCAAGCCTCTCTCAATCAACCAGGTACTT

Protein sequence

MSDPLERYRKLALRDSLAKSYRYPLACKELSFIIRAAFNKLPKNLQSLIYQDIITAFHLLPEMQTSSAVSAAHLLAQGVEAAWPKKKRNSAIVEFKKAMVAHKRRSKAHQEEKGSAQLPQDVLLHIFRFLDIESLVSAGLVCRSWNVAADDEFLWQLQYTTFFGSDDSAKLINNKGGVDDLASTPPHVDWKEAFKRAYIGNSLRRNTYGMGYCKHCNTIVFVSTLRCPVDHGKSKNKQIKPLSINQVL

Homology

[Close]

BLAST of MS006249 vs. NCBI nr
Match: XP_038874449.1 (F-box protein At5g52880 isoform X1 [Benincasa hispida])

HSP 1 Score: 416.4 bits (1069), Expect = 1.7e-112
Identity = 211/249 (84.74%), Postives = 225/249 (90.36%), Query Frame = 0


Query: 1   MSDPLERYRKLALRDSLAKSYRYPLACKELSFIIRAAFNKLPKNLQSLIYQDIITAFHLL 60
           MSDPLERY+ LAL DSLAKSY YPLACKELSF+IR AFNKLPKNLQSLIYQ IITAFHLL
Sbjct: 1   MSDPLERYQNLALTDSLAKSYSYPLACKELSFLIRGAFNKLPKNLQSLIYQHIITAFHLL 60

Query: 61  PEMQTSSAVSAAHLLAQGVEAAWPKKKRNSAIVEFKKAMVAHKRRSKAHQEEKGSAQLPQ 120
           PEMQTSSA SAAHLLAQGVEAA PK+KRNSAIVEFKKAMVAHKRRSKAHQEEKGSAQLPQ
Sbjct: 61  PEMQTSSAASAAHLLAQGVEAALPKQKRNSAIVEFKKAMVAHKRRSKAHQEEKGSAQLPQ 120

Query: 121 DVLLHIFRFLDIESLVSAGLVCRSWNVAADDEFLWQLQYTTFFG-SDDSAKLINNKGGVD 180
           DVLLHIFRFLD++SLVSAGLVCRSWNVAADDE+LWQLQYT FFG SDD++K IN+K  VD
Sbjct: 121 DVLLHIFRFLDVQSLVSAGLVCRSWNVAADDEYLWQLQYTKFFGCSDDNSKPINDKDSVD 180

Query: 181 DLASTPPHVDWKEAFKRAYIGNSLRRNTYGMGYCKHCNTIVFVSTLRCPVDHGKSKNKQI 240
           D AS   HVDWKE FK AYI NSLRRNT G GYC+HC+TIV  STLRCP DHG++KNKQI
Sbjct: 181 DFASA-RHVDWKEEFKSAYIVNSLRRNTNGRGYCRHCDTIVSSSTLRCPNDHGRNKNKQI 240

Query: 241 KPLSINQVL 249
           KPLSINQV+
Sbjct: 241 KPLSINQVV 248

[Close]

BLAST of MS006249 vs. NCBI nr
Match: XP_022973056.1 (F-box protein At5g52880 isoform X1 [Cucurbita maxima])

HSP 1 Score: 406.4 bits (1043), Expect = 1.8e-109
Identity = 205/248 (82.66%), Postives = 221/248 (89.11%), Query Frame = 0


Query: 1   MSDPLERYRKLALRDSLAKSYRYPLACKELSFIIRAAFNKLPKNLQSLIYQDIITAFHLL 60
           M+DPLERY+ LAL DSLAKSY YPLACKELSFI R AFNKLPKNLQSLI+Q IITAFHLL
Sbjct: 1   MTDPLERYQNLALTDSLAKSYSYPLACKELSFITRGAFNKLPKNLQSLIFQHIITAFHLL 60

Query: 61  PEMQTSSAVSAAHLLAQGVEAAWPKKKRNSAIVEFKKAMVAHKRRSKAHQEEKGSAQLPQ 120
           PEMQTSSAVSAAHLLAQGVEA+ PK+KRNSAIVEFKKAMVAHKRRSKA QE KGS+QLPQ
Sbjct: 61  PEMQTSSAVSAAHLLAQGVEASLPKQKRNSAIVEFKKAMVAHKRRSKARQEVKGSSQLPQ 120

Query: 121 DVLLHIFRFLDIESLVSAGLVCRSWNVAADDEFLWQLQYTTFFGSDDSAKLINNKGGVDD 180
           DVLLHIFRFLDIESLVSAGLVCRSW+ AADDE+LWQ QYTTFFG  D    IN++  VD 
Sbjct: 121 DVLLHIFRFLDIESLVSAGLVCRSWSAAADDEYLWQFQYTTFFGCSDEP--INDEDSVDG 180

Query: 181 LASTPPHVDWKEAFKRAYIGNSLRRNTYGMGYCKHCNTIVFVSTLRCPVDHGKSKNKQIK 240
           LAST PHVDWKEAFKRAY+GNSL RN YG GYCKHC+TIV  STLRCP DHG++KN+QIK
Sbjct: 181 LAST-PHVDWKEAFKRAYVGNSLWRNRYGRGYCKHCDTIVSFSTLRCPNDHGRNKNRQIK 240

Query: 241 PLSINQVL 249
           PLSINQV+
Sbjct: 241 PLSINQVV 245

[Close]

BLAST of MS006249 vs. NCBI nr
Match: XP_022922152.1 (F-box protein At5g52880 isoform X1 [Cucurbita moschata])

HSP 1 Score: 404.8 bits (1039), Expect = 5.2e-109
Identity = 206/248 (83.06%), Postives = 219/248 (88.31%), Query Frame = 0


Query: 1   MSDPLERYRKLALRDSLAKSYRYPLACKELSFIIRAAFNKLPKNLQSLIYQDIITAFHLL 60
           M+DPLERY+ LAL DSLAKSY YPLACKELSFI R AFNKLPKNLQSLI+Q IITAFHLL
Sbjct: 1   MTDPLERYQNLALTDSLAKSYSYPLACKELSFITRGAFNKLPKNLQSLIFQHIITAFHLL 60

Query: 61  PEMQTSSAVSAAHLLAQGVEAAWPKKKRNSAIVEFKKAMVAHKRRSKAHQEEKGSAQLPQ 120
           PEMQTSSAVSAAHLLAQGVEA+ PK+KRNSAIVEFKKAMVAHKRRSKA QE KGS+QLPQ
Sbjct: 61  PEMQTSSAVSAAHLLAQGVEASLPKQKRNSAIVEFKKAMVAHKRRSKARQEVKGSSQLPQ 120

Query: 121 DVLLHIFRFLDIESLVSAGLVCRSWNVAADDEFLWQLQYTTFFGSDDSAKLINNKGGVDD 180
           DVLLHIFRFLDIESLVSAGLVCRSWN AADDE+LWQ QYTTFFG  D    IN+K  VD 
Sbjct: 121 DVLLHIFRFLDIESLVSAGLVCRSWNAAADDEYLWQFQYTTFFGCSDEP--INDKDSVDG 180

Query: 181 LASTPPHVDWKEAFKRAYIGNSLRRNTYGMGYCKHCNTIVFVSTLRCPVDHGKSKNKQIK 240
           LAST PHVDWKEAFK AY+GNSL RN  G GYCKHC+TIV  STLRCP DHG++KNKQIK
Sbjct: 181 LAST-PHVDWKEAFKGAYVGNSLWRNRSGRGYCKHCDTIVSFSTLRCPNDHGRNKNKQIK 240

Query: 241 PLSINQVL 249
           PLSINQV+
Sbjct: 241 PLSINQVV 245

[Close]

BLAST of MS006249 vs. NCBI nr
Match: KAG7016844.1 (SPCC24B10.10c [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 404.8 bits (1039), Expect = 5.2e-109
Identity = 206/248 (83.06%), Postives = 219/248 (88.31%), Query Frame = 0


Query: 1    MSDPLERYRKLALRDSLAKSYRYPLACKELSFIIRAAFNKLPKNLQSLIYQDIITAFHLL 60
            M+DPLERY+ LAL DSLAKSY YPLACKELSFI R AFNKLPKNLQSLI+Q IITAFHLL
Sbjct: 825  MTDPLERYQNLALTDSLAKSYSYPLACKELSFITRGAFNKLPKNLQSLIFQHIITAFHLL 884

Query: 61   PEMQTSSAVSAAHLLAQGVEAAWPKKKRNSAIVEFKKAMVAHKRRSKAHQEEKGSAQLPQ 120
            PEMQTSSAVSAAHLLAQGVEA+ PK+KRNSAIVEFKKAMVAHKRRSKA QE KGS+QLPQ
Sbjct: 885  PEMQTSSAVSAAHLLAQGVEASLPKQKRNSAIVEFKKAMVAHKRRSKARQEVKGSSQLPQ 944

Query: 121  DVLLHIFRFLDIESLVSAGLVCRSWNVAADDEFLWQLQYTTFFGSDDSAKLINNKGGVDD 180
            DVLLHIFRFLDIESLVSAGLVCRSWN AADDE+LWQ QYTTFFG  D    IN+K  VD 
Sbjct: 945  DVLLHIFRFLDIESLVSAGLVCRSWNAAADDEYLWQFQYTTFFGCSDEP--INDKDSVDG 1004

Query: 181  LASTPPHVDWKEAFKRAYIGNSLRRNTYGMGYCKHCNTIVFVSTLRCPVDHGKSKNKQIK 240
            LAST PHVDWKEAFK AY+GNSL RN  G GYCKHC+TIV  STLRCP DHG++KNKQIK
Sbjct: 1005 LAST-PHVDWKEAFKGAYVGNSLWRNRSGRGYCKHCDTIVSFSTLRCPNDHGRNKNKQIK 1064

Query: 241  PLSINQVL 249
            PLSINQV+
Sbjct: 1065 PLSINQVV 1069

[Close]

BLAST of MS006249 vs. NCBI nr
Match: KAG6579359.1 (F-box protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 402.5 bits (1033), Expect = 2.6e-108
Identity = 205/246 (83.33%), Postives = 217/246 (88.21%), Query Frame = 0


Query: 3    DPLERYRKLALRDSLAKSYRYPLACKELSFIIRAAFNKLPKNLQSLIYQDIITAFHLLPE 62
            DPLERY+ LAL DSLAKSY YPLACKELSFI R AFNKLPKNLQSLI+Q IITAFHLLPE
Sbjct: 794  DPLERYQNLALTDSLAKSYSYPLACKELSFITRGAFNKLPKNLQSLIFQHIITAFHLLPE 853

Query: 63   MQTSSAVSAAHLLAQGVEAAWPKKKRNSAIVEFKKAMVAHKRRSKAHQEEKGSAQLPQDV 122
            MQTSSAVSAAHLLAQGVEA+ PK+KRNSAIVEFKKAMVAHKRRSKA QE KGS+QLPQDV
Sbjct: 854  MQTSSAVSAAHLLAQGVEASLPKQKRNSAIVEFKKAMVAHKRRSKARQEVKGSSQLPQDV 913

Query: 123  LLHIFRFLDIESLVSAGLVCRSWNVAADDEFLWQLQYTTFFGSDDSAKLINNKGGVDDLA 182
            LLHIFRFLDIESLVSAGLVCRSWN AADDE+LWQ QYTTFFG  D    IN+K  VD LA
Sbjct: 914  LLHIFRFLDIESLVSAGLVCRSWNAAADDEYLWQFQYTTFFGCSDEP--INDKDSVDGLA 973

Query: 183  STPPHVDWKEAFKRAYIGNSLRRNTYGMGYCKHCNTIVFVSTLRCPVDHGKSKNKQIKPL 242
            ST PHVDWKEAFK AY+GNSL RN  G GYCKHC+TIV  STLRCP DHG++KNKQIKPL
Sbjct: 974  ST-PHVDWKEAFKGAYVGNSLWRNRSGRGYCKHCDTIVSFSTLRCPNDHGRNKNKQIKPL 1033

Query: 243  SINQVL 249
            SINQV+
Sbjct: 1034 SINQVV 1036

[Close]

BLAST of MS006249 vs. ExPASy Swiss-Prot
Match: Q9FLX3 (F-box protein At5g52880 OS=Arabidopsis thaliana OX=3702 GN=At5g52880 PE=2 SV=1)

HSP 1 Score: 195.7 bits (496), Expect = 6.3e-49
Identity = 113/248 (45.56%), Postives = 158/248 (63.71%), Query Frame = 0


Query: 5   LERYRKLALRDSLAKSYRYPLACKELSFIIRAAFNKLPKNLQSLIYQDIITAFHLLPEMQ 64
           ++RY+ L + ++ +K+  YP AC ELS I+  A++  PKN+++LI+QD ++AF LLP++ 
Sbjct: 1   MKRYQNLKVGEAFSKNRIYPFACNELSSILNLAYSLSPKNVKALIFQDTLSAFRLLPDIN 60

Query: 65  TSSAVSAAHLLAQGVEAAWPKKKRNSAIVEFKKAMVAHKRRSKAHQEEK-GSAQLPQDVL 124
           TS+AVSAA+LL + VEA  PK+K+N AIVEFK+A VA KRRSK  +EE      LPQD+L
Sbjct: 61  TSAAVSAANLLLKSVEAVLPKQKKNLAIVEFKQAKVALKRRSKNREEEDIDIPSLPQDIL 120

Query: 125 LHIFRFLDIESLVSAGLVCRSWNVAADDEFLWQLQYTTFFGSDDSAKLINNKGGVDDLAS 184
           +HIF FL+I SLVS+  V RSWN A  +  LWQ Q+   F         N+K     L  
Sbjct: 121 IHIFSFLEISSLVSSAQVSRSWNQATHENSLWQSQFDLHF---------NHK----VLIR 180

Query: 185 TPPHVDWKEAFKRAYI-GNSLRRNTYGMGYCKHCNTIVFVSTLRC--PVDHGKSKNKQIK 244
               +DW+EAFK+AYI  NS      G GYC +C++IV+   LRC       KS NK + 
Sbjct: 181 MQSDIDWREAFKKAYIAANSSEALRSGRGYCSYCDSIVWHENLRCLNKQCRLKSGNKPLD 235

Query: 245 PLSINQVL 249
            ++ +QV+
Sbjct: 241 LITTHQVV 235

[Close]

BLAST of MS006249 vs. ExPASy Swiss-Prot
Match: Q969U6 (F-box/WD repeat-containing protein 5 OS=Homo sapiens OX=9606 GN=FBXW5 PE=1 SV=1)

HSP 1 Score: 54.3 bits (129), Expect = 2.3e-06
Identity = 27/88 (30.68%), Postives = 42/88 (47.73%), Query Frame = 0


Query: 111 EEKGSAQLPQDVLLHIFRFLDIESLVSAGLVCRSWNVAADDEFLWQLQYTTFFGSDDSAK 170
           +E G+  LP  ++  IF  L    +++AGLVCR W   + DEFLW+ Q+  ++       
Sbjct: 2   DEGGTPLLPDSLVYQIFLSLGPADVLAAGLVCRQWQAVSRDEFLWREQFYRYYQV----- 61

Query: 171 LINNKGGVDDLASTPPHVDWKEAFKRAY 199
                    D+   P  + W E F+R Y
Sbjct: 62  -------ARDVPRHPAAMSWYEEFQRLY 77

[Close]

BLAST of MS006249 vs. ExPASy Swiss-Prot
Match: Q4KLI9 (F-box/WD repeat-containing protein 5 OS=Rattus norvegicus OX=10116 GN=Fbxw5 PE=2 SV=1)

HSP 1 Score: 53.5 bits (127), Expect = 3.9e-06
Identity = 27/88 (30.68%), Postives = 40/88 (45.45%), Query Frame = 0


Query: 111 EEKGSAQLPQDVLLHIFRFLDIESLVSAGLVCRSWNVAADDEFLWQLQYTTFFGSDDSAK 170
           +E G   LP  ++  IF  L    +++AGLVCR W   + DEFLW+ Q+  ++       
Sbjct: 2   DEGGMPLLPDSLVYQIFLSLGPADVLAAGLVCRQWQAVSRDEFLWREQFYRYYQV----- 61

Query: 171 LINNKGGVDDLASTPPHVDWKEAFKRAY 199
                    D+   P    W E F+R Y
Sbjct: 62  -------ARDVPRHPAATSWYEEFRRLY 77

[Close]

BLAST of MS006249 vs. ExPASy Swiss-Prot
Match: Q9QXW2 (F-box/WD repeat-containing protein 5 OS=Mus musculus OX=10090 GN=Fbxw5 PE=1 SV=1)

HSP 1 Score: 53.1 bits (126), Expect = 5.1e-06
Identity = 27/88 (30.68%), Postives = 40/88 (45.45%), Query Frame = 0


Query: 111 EEKGSAQLPQDVLLHIFRFLDIESLVSAGLVCRSWNVAADDEFLWQLQYTTFFGSDDSAK 170
           +E G   LP  ++  IF  L    +++AGLVCR W   + DEFLW+ Q+  ++       
Sbjct: 2   DEGGLPLLPDSLVYQIFLSLGPADVLAAGLVCRQWQAVSRDEFLWKEQFYRYYQV----- 61

Query: 171 LINNKGGVDDLASTPPHVDWKEAFKRAY 199
                    D+   P    W E F+R Y
Sbjct: 62  -------ARDVPRHPAATSWYEEFRRLY 77

[Close]

BLAST of MS006249 vs. ExPASy Swiss-Prot
Match: Q5UPK8 (Putative F-box protein L127 OS=Acanthamoeba polyphaga mimivirus OX=212035 GN=MIMI_L127 PE=4 SV=1)

HSP 1 Score: 50.8 bits (120), Expect = 2.5e-05
Identity = 22/49 (44.90%), Postives = 31/49 (63.27%), Query Frame = 0


Query: 118 LPQDVLLHIFRFLDIESLVSAGLVCRSWNVAADDEFLWQLQYTTFFGSD 167
           L ++++ HIF FLD+ S +    VCR W   +DDE LW L Y  FFG++
Sbjct: 5   LSEEMVYHIFCFLDLSSFIKCSRVCRKWRRISDDEKLWNL-YGEFFGTN 52

[Close]

BLAST of MS006249 vs. ExPASy TrEMBL
Match: A0A6J1I7M6 (F-box protein At5g52880 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111471558 PE=4 SV=1)

HSP 1 Score: 406.4 bits (1043), Expect = 8.7e-110
Identity = 205/248 (82.66%), Postives = 221/248 (89.11%), Query Frame = 0


Query: 1   MSDPLERYRKLALRDSLAKSYRYPLACKELSFIIRAAFNKLPKNLQSLIYQDIITAFHLL 60
           M+DPLERY+ LAL DSLAKSY YPLACKELSFI R AFNKLPKNLQSLI+Q IITAFHLL
Sbjct: 1   MTDPLERYQNLALTDSLAKSYSYPLACKELSFITRGAFNKLPKNLQSLIFQHIITAFHLL 60

Query: 61  PEMQTSSAVSAAHLLAQGVEAAWPKKKRNSAIVEFKKAMVAHKRRSKAHQEEKGSAQLPQ 120
           PEMQTSSAVSAAHLLAQGVEA+ PK+KRNSAIVEFKKAMVAHKRRSKA QE KGS+QLPQ
Sbjct: 61  PEMQTSSAVSAAHLLAQGVEASLPKQKRNSAIVEFKKAMVAHKRRSKARQEVKGSSQLPQ 120

Query: 121 DVLLHIFRFLDIESLVSAGLVCRSWNVAADDEFLWQLQYTTFFGSDDSAKLINNKGGVDD 180
           DVLLHIFRFLDIESLVSAGLVCRSW+ AADDE+LWQ QYTTFFG  D    IN++  VD 
Sbjct: 121 DVLLHIFRFLDIESLVSAGLVCRSWSAAADDEYLWQFQYTTFFGCSDEP--INDEDSVDG 180

Query: 181 LASTPPHVDWKEAFKRAYIGNSLRRNTYGMGYCKHCNTIVFVSTLRCPVDHGKSKNKQIK 240
           LAST PHVDWKEAFKRAY+GNSL RN YG GYCKHC+TIV  STLRCP DHG++KN+QIK
Sbjct: 181 LAST-PHVDWKEAFKRAYVGNSLWRNRYGRGYCKHCDTIVSFSTLRCPNDHGRNKNRQIK 240

Query: 241 PLSINQVL 249
           PLSINQV+
Sbjct: 241 PLSINQVV 245

[Close]

BLAST of MS006249 vs. ExPASy TrEMBL
Match: A0A6J1E2E9 (F-box protein At5g52880 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111430216 PE=4 SV=1)

HSP 1 Score: 404.8 bits (1039), Expect = 2.5e-109
Identity = 206/248 (83.06%), Postives = 219/248 (88.31%), Query Frame = 0


Query: 1   MSDPLERYRKLALRDSLAKSYRYPLACKELSFIIRAAFNKLPKNLQSLIYQDIITAFHLL 60
           M+DPLERY+ LAL DSLAKSY YPLACKELSFI R AFNKLPKNLQSLI+Q IITAFHLL
Sbjct: 1   MTDPLERYQNLALTDSLAKSYSYPLACKELSFITRGAFNKLPKNLQSLIFQHIITAFHLL 60

Query: 61  PEMQTSSAVSAAHLLAQGVEAAWPKKKRNSAIVEFKKAMVAHKRRSKAHQEEKGSAQLPQ 120
           PEMQTSSAVSAAHLLAQGVEA+ PK+KRNSAIVEFKKAMVAHKRRSKA QE KGS+QLPQ
Sbjct: 61  PEMQTSSAVSAAHLLAQGVEASLPKQKRNSAIVEFKKAMVAHKRRSKARQEVKGSSQLPQ 120

Query: 121 DVLLHIFRFLDIESLVSAGLVCRSWNVAADDEFLWQLQYTTFFGSDDSAKLINNKGGVDD 180
           DVLLHIFRFLDIESLVSAGLVCRSWN AADDE+LWQ QYTTFFG  D    IN+K  VD 
Sbjct: 121 DVLLHIFRFLDIESLVSAGLVCRSWNAAADDEYLWQFQYTTFFGCSDEP--INDKDSVDG 180

Query: 181 LASTPPHVDWKEAFKRAYIGNSLRRNTYGMGYCKHCNTIVFVSTLRCPVDHGKSKNKQIK 240
           LAST PHVDWKEAFK AY+GNSL RN  G GYCKHC+TIV  STLRCP DHG++KNKQIK
Sbjct: 181 LAST-PHVDWKEAFKGAYVGNSLWRNRSGRGYCKHCDTIVSFSTLRCPNDHGRNKNKQIK 240

Query: 241 PLSINQVL 249
           PLSINQV+
Sbjct: 241 PLSINQVV 245

[Close]

BLAST of MS006249 vs. ExPASy TrEMBL
Match: A0A1S3AUV1 (F-box protein At5g52880 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103482926 PE=4 SV=1)

HSP 1 Score: 396.7 bits (1018), Expect = 6.9e-107
Identity = 200/250 (80.00%), Postives = 218/250 (87.20%), Query Frame = 0


Query: 1   MSDPLERYRKLALRDSLAKSYRYPLACKELSFIIRAAFNKLPKNLQSLIYQDIITAFHLL 60
           MS+PLERY KL+L DSL KSY YPLACKELSF+IR AF KLPKNLQSLIYQ IITAFHLL
Sbjct: 1   MSNPLERYHKLSLIDSLGKSYSYPLACKELSFLIRGAFIKLPKNLQSLIYQHIITAFHLL 60

Query: 61  PEMQTSSAVSAAHLLAQGVEAAWPKKKRNSAIVEFKKAMVAHKRRSKAHQEEKGSAQLPQ 120
           PEMQTSSA SAA LLAQ VEAA PK+KRNS IVE+KKAMV HKRRSKAHQEEKGS QLPQ
Sbjct: 61  PEMQTSSAASAARLLAQAVEAALPKQKRNSVIVEYKKAMVVHKRRSKAHQEEKGSCQLPQ 120

Query: 121 DVLLHIFRFLDIESLVSAGLVCRSWNVAADDEFLWQLQYTTFFG-SDDSAKLINNKGGVD 180
           DVLLHIFRFLD++SL SAGLVCRSWNVAADDE+LWQLQYTTFFG  DD++K IN+K   D
Sbjct: 121 DVLLHIFRFLDVQSLGSAGLVCRSWNVAADDEYLWQLQYTTFFGCPDDNSKPINDKDSED 180

Query: 181 DLA-STPPHVDWKEAFKRAYIGNSLRRNTYGMGYCKHCNTIVFVSTLRCPVDHGKSKNKQ 240
           D A S+ PHVDWKE FK+AY+GNSL RNT G GYCKHC+TIV  STLRC  DHG++KNKQ
Sbjct: 181 DFASSSTPHVDWKEEFKKAYVGNSLGRNTCGRGYCKHCDTIVSFSTLRCTNDHGRNKNKQ 240

Query: 241 IKPLSINQVL 249
           IKPLSINQV+
Sbjct: 241 IKPLSINQVV 250

[Close]

BLAST of MS006249 vs. ExPASy TrEMBL
Match: A0A1S3ATY4 (F-box protein At5g52880 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103482926 PE=4 SV=1)

HSP 1 Score: 396.4 bits (1017), Expect = 9.0e-107
Identity = 199/250 (79.60%), Postives = 218/250 (87.20%), Query Frame = 0


Query: 1   MSDPLERYRKLALRDSLAKSYRYPLACKELSFIIRAAFNKLPKNLQSLIYQDIITAFHLL 60
           MS+PLERY KL+L DSL KSY YPLACKELSF+IR AF KLPKNLQSLIYQ IITAFHLL
Sbjct: 1   MSNPLERYHKLSLIDSLGKSYSYPLACKELSFLIRGAFIKLPKNLQSLIYQHIITAFHLL 60

Query: 61  PEMQTSSAVSAAHLLAQGVEAAWPKKKRNSAIVEFKKAMVAHKRRSKAHQEEKGSAQLPQ 120
           PEMQTSSA SAA LLAQ VEAA PK+KRNS IVE+KKAMV HKRRSKAHQEEKGS QLPQ
Sbjct: 61  PEMQTSSAASAARLLAQAVEAALPKQKRNSVIVEYKKAMVVHKRRSKAHQEEKGSCQLPQ 120

Query: 121 DVLLHIFRFLDIESLVSAGLVCRSWNVAADDEFLWQLQYTTFFG-SDDSAKLINNKGGVD 180
           DVLLHIFRFLD++SL SAGLVCRSWNVAADDE+LWQLQYTTFFG  DD++K IN+K   D
Sbjct: 121 DVLLHIFRFLDVQSLGSAGLVCRSWNVAADDEYLWQLQYTTFFGCPDDNSKPINDKDSED 180

Query: 181 DLA-STPPHVDWKEAFKRAYIGNSLRRNTYGMGYCKHCNTIVFVSTLRCPVDHGKSKNKQ 240
           D A S+ PHVDWKE FK+AY+GNSL RNT G GYCKHC+TIV  STLRC  DHG++KNKQ
Sbjct: 181 DFASSSTPHVDWKEEFKKAYVGNSLGRNTCGRGYCKHCDTIVSFSTLRCTNDHGRNKNKQ 240

Query: 241 IKPLSINQVL 249
           IKPLSINQ++
Sbjct: 241 IKPLSINQIV 250

[Close]

BLAST of MS006249 vs. ExPASy TrEMBL
Match: A0A0A0KNE5 (F-box domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G139890 PE=4 SV=1)

HSP 1 Score: 391.3 bits (1004), Expect = 2.9e-105
Identity = 196/250 (78.40%), Postives = 217/250 (86.80%), Query Frame = 0


Query: 1   MSDPLERYRKLALRDSLAKSYRYPLACKELSFIIRAAFNKLPKNLQSLIYQDIITAFHLL 60
           MS+PLERY KL L D LAKSY YPLACKELSF+IR AF KLPKNLQSLIY+ IITAFHLL
Sbjct: 1   MSNPLERYHKLCLMDFLAKSYSYPLACKELSFLIRGAFIKLPKNLQSLIYEHIITAFHLL 60

Query: 61  PEMQTSSAVSAAHLLAQGVEAAWPKKKRNSAIVEFKKAMVAHKRRSKAHQEEKGSAQLPQ 120
           PEMQTSSA SAA LLA+ VEAA PK+KRNS IVEFKKAMV HKRR+KAHQEEKGS QLPQ
Sbjct: 61  PEMQTSSAASAARLLARAVEAALPKQKRNSVIVEFKKAMVVHKRRTKAHQEEKGSCQLPQ 120

Query: 121 DVLLHIFRFLDIESLVSAGLVCRSWNVAADDEFLWQLQYTTFF-GSDDSAKLINNKGGVD 180
           DVLLHIFRF+D++SLVSAGLVCRSWNVAA+DE+LWQLQYTTFF  SDD++K IN+K   D
Sbjct: 121 DVLLHIFRFIDVQSLVSAGLVCRSWNVAAEDEYLWQLQYTTFFVCSDDNSKSINDKDSED 180

Query: 181 DLA-STPPHVDWKEAFKRAYIGNSLRRNTYGMGYCKHCNTIVFVSTLRCPVDHGKSKNKQ 240
             A S+ P VDWKE FK+AY+GNSL RNTYG GYCKHC+TIV  STLRCP DHG++KN Q
Sbjct: 181 GFASSSTPRVDWKEEFKKAYVGNSLGRNTYGRGYCKHCDTIVSFSTLRCPNDHGRNKNTQ 240

Query: 241 IKPLSINQVL 249
           IKPLSINQV+
Sbjct: 241 IKPLSINQVV 250

[Close]

BLAST of MS006249 vs. TAIR 10
Match: AT5G52880.1 (F-box family protein )

HSP 1 Score: 195.7 bits (496), Expect = 4.5e-50
Identity = 113/248 (45.56%), Postives = 158/248 (63.71%), Query Frame = 0


Query: 5   LERYRKLALRDSLAKSYRYPLACKELSFIIRAAFNKLPKNLQSLIYQDIITAFHLLPEMQ 64
           ++RY+ L + ++ +K+  YP AC ELS I+  A++  PKN+++LI+QD ++AF LLP++ 
Sbjct: 1   MKRYQNLKVGEAFSKNRIYPFACNELSSILNLAYSLSPKNVKALIFQDTLSAFRLLPDIN 60

Query: 65  TSSAVSAAHLLAQGVEAAWPKKKRNSAIVEFKKAMVAHKRRSKAHQEEK-GSAQLPQDVL 124
           TS+AVSAA+LL + VEA  PK+K+N AIVEFK+A VA KRRSK  +EE      LPQD+L
Sbjct: 61  TSAAVSAANLLLKSVEAVLPKQKKNLAIVEFKQAKVALKRRSKNREEEDIDIPSLPQDIL 120

Query: 125 LHIFRFLDIESLVSAGLVCRSWNVAADDEFLWQLQYTTFFGSDDSAKLINNKGGVDDLAS 184
           +HIF FL+I SLVS+  V RSWN A  +  LWQ Q+   F         N+K     L  
Sbjct: 121 IHIFSFLEISSLVSSAQVSRSWNQATHENSLWQSQFDLHF---------NHK----VLIR 180

Query: 185 TPPHVDWKEAFKRAYI-GNSLRRNTYGMGYCKHCNTIVFVSTLRC--PVDHGKSKNKQIK 244
               +DW+EAFK+AYI  NS      G GYC +C++IV+   LRC       KS NK + 
Sbjct: 181 MQSDIDWREAFKKAYIAANSSEALRSGRGYCSYCDSIVWHENLRCLNKQCRLKSGNKPLD 235

Query: 245 PLSINQVL 249
            ++ +QV+
Sbjct: 241 LITTHQVV 235

[Close]

BLAST of MS006249 vs. TAIR 10
Match: AT5G45360.1 (F-box family protein )

HSP 1 Score: 45.8 bits (107), Expect = 5.8e-05
Identity = 25/64 (39.06%), Postives = 36/64 (56.25%), Query Frame = 0


Query: 102 HKRRSKAHQEEKG-SAQLPQDVLLHIFRFLDIESLVSAGLVCRSWNVAADDEFLWQLQYT 161
           +K ++ A + E G    +P ++  HI +FL  E LVS  LVC+  N AA DE LW+  Y 
Sbjct: 57  NKDKTDAKRIESGVFTNVPTELFRHILKFLSSEDLVSCSLVCKFLNFAAADESLWRRLYC 116

Query: 162 TFFG 165
             +G
Sbjct: 117 IRWG 120

The following BLAST results are available for this feature:

BLAST of MS006249 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038874449.1	1.7e-112	84.74	F-box protein At5g52880 isoform X1 [Benincasa hispida]	[more]
XP_022973056.1	1.8e-109	82.66	F-box protein At5g52880 isoform X1 [Cucurbita maxima]	[more]
XP_022922152.1	5.2e-109	83.06	F-box protein At5g52880 isoform X1 [Cucurbita moschata]	[more]
KAG7016844.1	5.2e-109	83.06	SPCC24B10.10c [Cucurbita argyrosperma subsp. argyrosperma]	[more]
KAG6579359.1	2.6e-108	83.33	F-box protein, partial [Cucurbita argyrosperma subsp. sororia]	[more]

BLAST of MS006249 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FLX3	6.3e-49	45.56	F-box protein At5g52880 OS=Arabidopsis thaliana OX=3702 GN=At5g52880 PE=2 SV=1	[more]
Q969U6	2.3e-06	30.68	F-box/WD repeat-containing protein 5 OS=Homo sapiens OX=9606 GN=FBXW5 PE=1 SV=1	[more]
Q4KLI9	3.9e-06	30.68	F-box/WD repeat-containing protein 5 OS=Rattus norvegicus OX=10116 GN=Fbxw5 PE=2...	[more]
Q9QXW2	5.1e-06	30.68	F-box/WD repeat-containing protein 5 OS=Mus musculus OX=10090 GN=Fbxw5 PE=1 SV=1	[more]
Q5UPK8	2.5e-05	44.90	Putative F-box protein L127 OS=Acanthamoeba polyphaga mimivirus OX=212035 GN=MIM...	[more]

BLAST of MS006249 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1I7M6	8.7e-110	82.66	F-box protein At5g52880 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111471558 P...	[more]
A0A6J1E2E9	2.5e-109	83.06	F-box protein At5g52880 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111430216...	[more]
A0A1S3AUV1	6.9e-107	80.00	F-box protein At5g52880 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103482926 PE=4 ...	[more]
A0A1S3ATY4	9.0e-107	79.60	F-box protein At5g52880 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103482926 PE=4 ...	[more]
A0A0A0KNE5	2.9e-105	78.40	F-box domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G139890 PE=4 ...	[more]

BLAST of MS006249 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G52880.1	4.5e-50	45.56	F-box family protein	[more]
AT5G45360.1	5.8e-05	39.06	F-box family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001810	F-box domain	SMART	SM00256	fbox_2	coord: 118..158 e-value: 4.3E-8 score: 42.9
IPR001810	F-box domain	PFAM	PF12937	F-box-like	coord: 117..159 e-value: 9.4E-14 score: 51.0
IPR001810	F-box domain	PROSITE	PS50181	FBOX	coord: 112..158 score: 11.722999
None	No IPR available	GENE3D	1.20.1280.50		coord: 99..206 e-value: 1.3E-14 score: 55.9
None	No IPR available	PANTHER	PTHR47744	OS05G0526300 PROTEIN	coord: 1..247
IPR036047	F-box-like domain superfamily	SUPERFAMILY	81383	F-box domain	coord: 116..198

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS006249.1	MS006249.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005515	protein binding