MS006205 (gene) Bitter gourd (TR) v1

BLAST of MS006205 vs. NCBI nr
Match: XP_022995844.1 (succinate dehydrogenase assembly factor 4, mitochondrial [Cucurbita maxima])

HSP 1 Score: 159.8 bits (403), Expect = 1.4e-35
Identity = 90/114 (78.95%), Postives = 101/114 (88.60%), Query Frame = 0

Query: 1   MSSNLSRLFSSLQSLSALPAVSRVTSNSLSRLVSSSANQQPSRSQQGVPGEEQREAGKES 60
           MSSNL+RLFS   SL+AL AV+RVTS S+SRLVSSSANQQPSR +QGVPGEEQREAGK S
Sbjct: 1   MSSNLTRLFSP-PSLTALSAVNRVTSTSVSRLVSSSANQQPSRLEQGVPGEEQREAGKGS 60

Query: 61  LPNQHEPAGDDQDDDDDDDGGVDLNEATGEVGGPKGPEPTRYGDWERNGRCYDF 115
           LP+QHE AGDD+D +D+   G D+N+ATGEVGGPKGPEPTRYGDWERNGRCYDF
Sbjct: 61  LPDQHEAAGDDRDSEDE---GADVNKATGEVGGPKGPEPTRYGDWERNGRCYDF 110

BLAST of MS006205 vs. NCBI nr
Match: XP_022957978.1 (succinate dehydrogenase assembly factor 4, mitochondrial [Cucurbita moschata])

HSP 1 Score: 157.1 bits (396), Expect = 8.8e-35
Identity = 89/114 (78.07%), Postives = 100/114 (87.72%), Query Frame = 0

Query: 1   MSSNLSRLFSSLQSLSALPAVSRVTSNSLSRLVSSSANQQPSRSQQGVPGEEQREAGKES 60
           MSSNL+RLFS   SL+AL AV+R TS S+SRLVSSSANQQPSR +QGVPGEEQREAGK S
Sbjct: 1   MSSNLTRLFSP-PSLTALSAVNRATSISVSRLVSSSANQQPSRLEQGVPGEEQREAGKGS 60

Query: 61  LPNQHEPAGDDQDDDDDDDGGVDLNEATGEVGGPKGPEPTRYGDWERNGRCYDF 115
           LP+QHE AGDD+D +D+   G D+N+ATGEVGGPKGPEPTRYGDWERNGRCYDF
Sbjct: 61  LPDQHEAAGDDRDSEDE---GADVNKATGEVGGPKGPEPTRYGDWERNGRCYDF 110

BLAST of MS006205 vs. NCBI nr
Match: XP_023532848.1 (succinate dehydrogenase assembly factor 4, mitochondrial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 156.0 bits (393), Expect = 2.0e-34
Identity = 88/114 (77.19%), Postives = 100/114 (87.72%), Query Frame = 0

Query: 1   MSSNLSRLFSSLQSLSALPAVSRVTSNSLSRLVSSSANQQPSRSQQGVPGEEQREAGKES 60
           MSSNL+RLFS   SL++L AV+R TS S+SRLVSSSANQQPSR +QGVPGEEQREAGK S
Sbjct: 1   MSSNLTRLFSP-PSLTSLSAVNRATSISVSRLVSSSANQQPSRLEQGVPGEEQREAGKGS 60

Query: 61  LPNQHEPAGDDQDDDDDDDGGVDLNEATGEVGGPKGPEPTRYGDWERNGRCYDF 115
           LP+QHE AGDD+D +D+   G D+N+ATGEVGGPKGPEPTRYGDWERNGRCYDF
Sbjct: 61  LPDQHEAAGDDRDSEDE---GADVNKATGEVGGPKGPEPTRYGDWERNGRCYDF 110

BLAST of MS006205 vs. NCBI nr
Match: KAG6605777.1 (Succinate dehydrogenase assembly factor 4, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia] >KAG7035745.1 Succinate dehydrogenase assembly factor 4, mitochondrial, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 156.0 bits (393), Expect = 2.0e-34
Identity = 88/114 (77.19%), Postives = 100/114 (87.72%), Query Frame = 0

Query: 1   MSSNLSRLFSSLQSLSALPAVSRVTSNSLSRLVSSSANQQPSRSQQGVPGEEQREAGKES 60
           MSSNL+RLFS   SL+AL AV+R TS S+SRLVSSSANQQPSR +QGVPG+EQREAGK S
Sbjct: 1   MSSNLTRLFSP-PSLTALSAVNRATSISVSRLVSSSANQQPSRLEQGVPGDEQREAGKGS 60

Query: 61  LPNQHEPAGDDQDDDDDDDGGVDLNEATGEVGGPKGPEPTRYGDWERNGRCYDF 115
           LP+QHE AGDD+D +D+   G D+N+ATGEVGGPKGPEPTRYGDWERNGRCYDF
Sbjct: 61  LPDQHEAAGDDRDSEDE---GADVNKATGEVGGPKGPEPTRYGDWERNGRCYDF 110

BLAST of MS006205 vs. NCBI nr
Match: KGN49871.1 (hypothetical protein Csa_000062 [Cucumis sativus])

HSP 1 Score: 142.5 bits (358), Expect = 2.2e-30
Identity = 82/114 (71.93%), Postives = 92/114 (80.70%), Query Frame = 0

Query: 1   MSSNLSRLFSSLQSLSALPAVSRVTSNSLSRLVSSSANQQPSRSQQGVPGEEQREAGKES 60
           MSSNLSRLFSS  +LSALPAVSR+T NSLSR VSSS+NQQPSR ++GV  EEQREAGK S
Sbjct: 1   MSSNLSRLFSSPPTLSALPAVSRLTFNSLSRFVSSSSNQQPSRLEKGVSSEEQREAGKGS 60

Query: 61  LPNQHEPAGDDQDDDDDDDGGVDLNEATGEVGGPKGPEPTRYGDWERNGRCYDF 115
            PN+HE AGDD+   D+   GVD  + T E+GGPKGPEPTRYGDWER GRC DF
Sbjct: 61  SPNRHEAAGDDRGGQDE---GVDTEKMTREIGGPKGPEPTRYGDWERKGRCSDF 111

BLAST of MS006205 vs. ExPASy Swiss-Prot
Match: Q9VLU6 (Succinate dehydrogenase assembly factor 4, mitochondrial OS=Drosophila melanogaster OX=7227 GN=Sirup PE=3 SV=2)

HSP 1 Score: 55.5 bits (132), Expect = 4.7e-07
Identity = 23/30 (76.67%), Postives = 24/30 (80.00%), Query Frame = 0

Query: 85  NEATGEVGGPKGPEPTRYGDWERNGRCYDF 115
           N  TGE+GGP GPEPTRYGDWER GR  DF
Sbjct: 89  NPYTGEIGGPAGPEPTRYGDWERKGRVSDF 118

BLAST of MS006205 vs. ExPASy Swiss-Prot
Match: Q8BTE0 (Succinate dehydrogenase assembly factor 4, mitochondrial OS=Mus musculus OX=10090 GN=Sdhaf4 PE=3 SV=2)

HSP 1 Score: 51.6 bits (122), Expect = 6.8e-06
Identity = 33/71 (46.48%), Postives = 41/71 (57.75%), Query Frame = 0

Query: 51  EEQREAGKESLPNQHEPAG--DDQDDDDDDDGGV-----DLNEATGEVGGPKGPEPTRYG 110
           E + E  K++L     P G  D  +D  ++   +     D+N  T E GGPKGPEPTRYG
Sbjct: 34  EGKPEPAKQALKKSKLPLGRFDSLEDSPEEREPLQKFPDDVNPVTKEKGGPKGPEPTRYG 93

Query: 111 DWERNGRCYDF 115
           DWER GRC DF
Sbjct: 94  DWERKGRCIDF 104

BLAST of MS006205 vs. ExPASy Swiss-Prot
Match: Q54Y25 (Succinate dehydrogenase assembly factor 4, mitochondrial OS=Dictyostelium discoideum OX=44689 GN=DDB_G0278459 PE=3 SV=1)

HSP 1 Score: 47.4 bits (111), Expect = 1.3e-04
Identity = 36/112 (32.14%), Postives = 62/112 (55.36%), Query Frame = 0

Query: 3   SNLSRLFSSLQSLSALPAVSRVTSNSLSRLVSSSANQQPSRSQQGVPGEEQREAGKESLP 62
           +NL   F+ +  L+A    +++  +  S  ++++ N   + +   +   ++ E  KE+  
Sbjct: 2   NNLISKFNKIIYLNATKQTNKINCSFYSTTINNNNNNNNNNNNNNI-SNKKAEMSKEN-- 61

Query: 63  NQHEPAGDDQDDDDDDDGGVDLNEATGEVGGPKGPEPTRYGDWERNGRCYDF 115
              +   D +++ D+D+    +N  T E+GGPKGPEPTRY DWERNGR  DF
Sbjct: 62  --QQLLQDLEEEFDEDEFKPYVNPITKEIGGPKGPEPTRYNDWERNGRVSDF 108

BLAST of MS006205 vs. ExPASy Swiss-Prot
Match: Q5VUM1 (Succinate dehydrogenase assembly factor 4, mitochondrial OS=Homo sapiens OX=9606 GN=SDHAF4 PE=1 SV=1)

HSP 1 Score: 46.6 bits (109), Expect = 2.2e-04
Identity = 33/78 (42.31%), Postives = 43/78 (55.13%), Query Frame = 0

Query: 39  QQPSRSQQGVPGEEQREAGKESLPNQHEPAGDDQ--DDDDDDDGGVDLNEATGEVGGPKG 98
           ++ S SQ G     ++   K  LP     A +D   + +  +    D+N  T E GGP+G
Sbjct: 31  RKTSSSQGGKSELVKQSLKKPKLPEGRFDAPEDSHLEKEPLEKFPDDVNPVTKEKGGPRG 90

Query: 99  PEPTRYGDWERNGRCYDF 115
           PEPTRYGDWER GRC DF
Sbjct: 91  PEPTRYGDWERKGRCIDF 108

BLAST of MS006205 vs. ExPASy TrEMBL
Match: A0A6J1K722 (Succinate dehydrogenase assembly factor 4, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111491251 PE=3 SV=1)

HSP 1 Score: 159.8 bits (403), Expect = 6.5e-36
Identity = 90/114 (78.95%), Postives = 101/114 (88.60%), Query Frame = 0

Query: 1   MSSNLSRLFSSLQSLSALPAVSRVTSNSLSRLVSSSANQQPSRSQQGVPGEEQREAGKES 60
           MSSNL+RLFS   SL+AL AV+RVTS S+SRLVSSSANQQPSR +QGVPGEEQREAGK S
Sbjct: 1   MSSNLTRLFSP-PSLTALSAVNRVTSTSVSRLVSSSANQQPSRLEQGVPGEEQREAGKGS 60

Query: 61  LPNQHEPAGDDQDDDDDDDGGVDLNEATGEVGGPKGPEPTRYGDWERNGRCYDF 115
           LP+QHE AGDD+D +D+   G D+N+ATGEVGGPKGPEPTRYGDWERNGRCYDF
Sbjct: 61  LPDQHEAAGDDRDSEDE---GADVNKATGEVGGPKGPEPTRYGDWERNGRCYDF 110

BLAST of MS006205 vs. ExPASy TrEMBL
Match: A0A6J1H1T7 (Succinate dehydrogenase assembly factor 4, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111459345 PE=3 SV=1)

HSP 1 Score: 157.1 bits (396), Expect = 4.2e-35
Identity = 89/114 (78.07%), Postives = 100/114 (87.72%), Query Frame = 0

Query: 1   MSSNLSRLFSSLQSLSALPAVSRVTSNSLSRLVSSSANQQPSRSQQGVPGEEQREAGKES 60
           MSSNL+RLFS   SL+AL AV+R TS S+SRLVSSSANQQPSR +QGVPGEEQREAGK S
Sbjct: 1   MSSNLTRLFSP-PSLTALSAVNRATSISVSRLVSSSANQQPSRLEQGVPGEEQREAGKGS 60

Query: 61  LPNQHEPAGDDQDDDDDDDGGVDLNEATGEVGGPKGPEPTRYGDWERNGRCYDF 115
           LP+QHE AGDD+D +D+   G D+N+ATGEVGGPKGPEPTRYGDWERNGRCYDF
Sbjct: 61  LPDQHEAAGDDRDSEDE---GADVNKATGEVGGPKGPEPTRYGDWERNGRCYDF 110

BLAST of MS006205 vs. ExPASy TrEMBL
Match: A0A0A0KQ66 (Succinate dehydrogenase assembly factor 4, mitochondrial OS=Cucumis sativus OX=3659 GN=Csa_5G139490 PE=3 SV=1)

HSP 1 Score: 142.5 bits (358), Expect = 1.1e-30
Identity = 82/114 (71.93%), Postives = 92/114 (80.70%), Query Frame = 0

Query: 1   MSSNLSRLFSSLQSLSALPAVSRVTSNSLSRLVSSSANQQPSRSQQGVPGEEQREAGKES 60
           MSSNLSRLFSS  +LSALPAVSR+T NSLSR VSSS+NQQPSR ++GV  EEQREAGK S
Sbjct: 1   MSSNLSRLFSSPPTLSALPAVSRLTFNSLSRFVSSSSNQQPSRLEKGVSSEEQREAGKGS 60

Query: 61  LPNQHEPAGDDQDDDDDDDGGVDLNEATGEVGGPKGPEPTRYGDWERNGRCYDF 115
            PN+HE AGDD+   D+   GVD  + T E+GGPKGPEPTRYGDWER GRC DF
Sbjct: 61  SPNRHEAAGDDRGGQDE---GVDTEKMTREIGGPKGPEPTRYGDWERKGRCSDF 111

BLAST of MS006205 vs. ExPASy TrEMBL
Match: A0A5A7TMD3 (Succinate dehydrogenase assembly factor 4, mitochondrial OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold376G00530 PE=3 SV=1)

HSP 1 Score: 142.1 bits (357), Expect = 1.4e-30
Identity = 82/114 (71.93%), Postives = 90/114 (78.95%), Query Frame = 0

Query: 1   MSSNLSRLFSSLQSLSALPAVSRVTSNSLSRLVSSSANQQPSRSQQGVPGEEQREAGKES 60
           MSS+LSRLFSS  +LSALP V R+TSNSLSR VSSS+NQQPSRS+QGV  EEQREA K S
Sbjct: 1   MSSHLSRLFSSPPTLSALPTVGRLTSNSLSRFVSSSSNQQPSRSEQGVSSEEQREAEKGS 60

Query: 61  LPNQHEPAGDDQDDDDDDDGGVDLNEATGEVGGPKGPEPTRYGDWERNGRCYDF 115
            PNQHE AGDD+   D    GVD  +   E+GGPKG EPTRYGDWERNGRCYDF
Sbjct: 61  SPNQHEAAGDDRGSQDQ---GVDTEKMAPEIGGPKGHEPTRYGDWERNGRCYDF 111

BLAST of MS006205 vs. ExPASy TrEMBL
Match: W9RP47 (Succinate dehydrogenase assembly factor 4, mitochondrial OS=Morus notabilis OX=981085 GN=L484_000138 PE=3 SV=1)

HSP 1 Score: 95.9 bits (237), Expect = 1.2e-16
Identity = 65/123 (52.85%), Postives = 81/123 (65.85%), Query Frame = 0

Query: 1   MSSNLSRLFSSLQSLSALPAV---------SRVTSNSLSRLVSSSANQQPSRSQQGVPGE 60
           M+SNL RLFSS+ +LS    +         +R  +NS SRLVSSSA Q+P   Q+ +  +
Sbjct: 1   MASNLGRLFSSITNLSTPKVILSSTRSEPLTRCATNSASRLVSSSA-QKPQYDQENM-NK 60

Query: 61  EQREAGKESLPNQHEPAGDDQDDDDDDDGGVDLNEATGEVGGPKGPEPTRYGDWERNGRC 115
           EQREA  + L N+ E      D  +D++ G  LNE TGEVGGP+GPEPTRYGDWERNGRC
Sbjct: 61  EQREAIAKDLENEKE------DGVEDEEEGTSLNEQTGEVGGPRGPEPTRYGDWERNGRC 115

BLAST of MS006205 vs. TAIR 10
Match: AT5G67490.1 (unknown protein; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF1674 (InterPro:IPR012875); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 52.0 bits (123), Expect = 3.7e-07
Identity = 43/115 (37.39%), Postives = 67/115 (58.26%), Query Frame = 0

Query: 2   SSNLSRLFSSLQSLSA--LPAVSRVTSNSLSRLVSSSANQQPSRSQQGVPGEEQREAGKE 61
           ++N+ RL +  +S+S+  L  VSR  S+      +    Q PS +Q      +  +  ++
Sbjct: 3   TNNIVRLIAPSRSVSSRFLEPVSRFLSSG-----TPPPPQAPSPNQDLNRDVKPDQNLQQ 62

Query: 62  SLPNQHEPAGDDQDDDDDDDGGVDLNEATGEVGGPKGPEPTRYGDWERNGRCYDF 115
           +L  Q E    ++++ +   GG  +NE TGE+GGP+GPEPTRYGDWE+ GRC DF
Sbjct: 63  NLQMQKE----EEEEGEGGGGGEFVNEDTGEIGGPRGPEPTRYGDWEQRGRCSDF 108