MS006173 (gene) Bitter gourd (TR) v1

Overview
NameMS006173
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionYpt/Rab-GAP domain of gyp1p superfamily protein
Locationscaffold96: 866226 .. 869884 (+)
RNA-Seq ExpressionMS006173
SyntenyMS006173
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
AGGTGTCGTCTCAAATCACCATGGTCGCGCAGAAAAAGAAAAAATGCACTTTCATCTCGACAATGGAGAACTGTGTTTAATCCAGATGGGAAACTCCGGGATGGTGGAATTAAGTTTGTAAAAAAAGTTCGCAGTGGAGTAAGTCAACATTATGTTCGACTATCTTATGAAAAATATTATGTTTGTCTGTTTATTGCTCTTCTCAGCTCGAGTATGTTATCTCAATTCCTTAGTTCCTGTTGTTGAATTAAAATATTAATGCAATTTTTGATTTAGGGTGTTGATCCAAGTATTCGGGCAGAGGTTTGGCCTTTCCTTCTTGGAGTGTAAGTATCACTCACCTTTGCGTCTTGTCTATTACCTCCATTATAAATGGCCTATATGTTTGAAGTAGAGCTGTTCTACTCTCTCGTGCATGTATTGCCTGTTCTAAAGTTATTTATTTCATGGACAGTATCTTCAATACAATCAAAATTGTTAAATTATTTGTTCTTTTACTTATTATTAAATTTTATCAGTTTGTTTCTAGGAATTAGAATATATCTTACACTGGTCAAGTATCTTGTACAACTCCAGCCATTTCCATTGGTCAATGTCAATGAATCTTAATTAGATGACCTGATCAAGCAAGGTCAAGTATCTTACTCAATTTAAGAGAGATCCATCAATCATGGTCAATGTTTTAAAAGGCTAAAAGTTAGAAAAGGCTAAGCCTCGAGGCAGCATGAGAAGTAAGTCTCAATTAAAAAATATATATATCGAAAAATTATAAAATTGTGTAATCAAGTATTAATATACACAATTGTACAAAAAAAAAGGTCTTAATAAACACAATCATATGAAAGTACCAAAAAGAAACCATTTCAATCTTTTTTTTGAAAGGATAAAAAGAAATAATGTCTATCAATTCTCACTTAAATAAATAAAGATCCTAAAAGGTAATTCTAAACTTTATTATCATCACAAATTTTTCTTAGATGGACTATTTTCAGATATTTCATAAATCATACCATCAATAAAATATTTGATTCCTATTTTAAAACATCACTAGGTTTTCTTTAATTTTTTTTTTACTTCAGTGTCCTCCAAAACCTTAGCTGTGAAAGTAATAATGCACCAACTTACCACAGCTCATGATATAATAGCAGCACACAAATAATAGCGGACTTGAACATAGTTCAATTGGTGAAGGTACTACTACTTACCTCCTTAAAGGTTAGAGGTTCAAAGCCCCACCTCTTATTGAACTTTGAAAAAGGAAGAAGAATAGAAAACGAGTAGTAAAACTTGGCTACTCTTCCGACCATTGTATGAGGCATAAAGAATCTGAACATTTGTGTGCTGTGATTGGAAGCGCTAATCTCTTCTATAAGAGGAGCCTCTTATGATTCAAAGAAGGCATAAGTCTAGAAGTTTGAGCCTTGATGACATATATTGTTTGAAATCATTATTAAAAATTTTAAATATATGAATATTTTTAAATGATGTGTCATTGTCCTGTTCGTTTCCTAAGTTTTTTGTAGAATGACATGTTAGATGAGTCATTAATTAATAATTGGAGTTGAGGTGTCTGATCCCATACTGTAGGGAATACCTTTTCCTTGCATCACAAACACAAACATATGCACAAAGAAAGAGAAAGTTGTATCTTTACAGTTTGCTTCAAAATGTCTTGTTAATTATTTGTTGCACGCCTGAACTTGGAGGTATGTGGAATATTTGACAAGAATTAGAAAGCCCTTATCATTGCGTTTAGTATCTAATTTCATTACATTATTTGATTTAAATTGCCCGATTCAGCTGTTTGGTGAGTGTTGGGTACTACTGGGTAGTGATTAGATGGTCAATGAGAAAGACAATGTATTATTGAAGGAGGGGAAGTTAGGCCCTTGTAAATATCGGTCCAAAACTTTAGCAAAATGTAACTGGGATATCATTCCAAGTGATTTGCTCAACCAGCTTAGCACTTCTTCAGGGATAAATTGCGGTTTATGTGCTGCTTTAGTTATATTGATATTTGTGTGACTGCTTTAGTTATATTGGTATTTTTATGAACTCTGTTGAACAATGCATTATTTAGGCCTTAAATTGATCTACGTCTATATTGCTTTAAAGCTCCGTATTACTGTGGTGCTCTAAATACTAGAAACGCAGATTTTCTACTTTACATGCATTGATTTTATTTGACTGACCCTATAACTTTTAACAGCTATGACCTCAAGAGTTCTAAAGAGGAAAGGGATATTATAAAGACTCAAAAAAGGTGCTTAGTATATCTTTTTAAATATTTTTTATTTAGTATTTATGCTGGATTCTTTATGCATTACTTTAGGTTGTTGCAAGATGTCTCTCTCTCTTCAGTGACATCCATCTATGAGGATTTTATTTTACAATTATAATGTTTTTGGTGCAGAAAGGAATATGAAAAACTCCGTAAACAGTGCAGGCGATTGATAAAACGTAGGAATGAAAGCTCTAAATGGAATGAATTTGGGGACATGATTGTCGGGGAAGATGGCTTTCGCTTTCTTGTGCGAGACATAGATTCTCCTAGTTCTGAAGATGTGGTTAGTGCTAGAGAGTCTCTTTCGAGTGAAGAAAGGTGCTCAAATGTTGAATTTTTGGATGAACCCTTCTGCTCTTTGTTGGAAGGGGAGGGGAGTTCAAGACAGATCGCAGCTGATGGTTCTTTGGTACTGAATTCGGACTCCTCTGACTCAGACTCCTCAGACGACTTGGATGTTAGTCAGACTTTTCCCCCCACAGATGGAATGGAAGAACCTTATCTTGATATGACGCCTAAGGAAAATTCTTCTCCCCCTAGGACTGAGGTCTCATCAAAGCTCCATAATAGTGAAGACTTTGCAACATGGCAGCGGATTATCCGACTTGATGCACTTCGTTCTAACTCAGAATGGGTACCATACTTGACATCTCAAGCAATGGTTTCGGATGGCAGAGCCCGGCGGTCTGCTGAGGCTGTTGGTTTAGTTGATTATGACCATCTAGAACCTTGTATGATCTTCCATGCTGCAAGACTAGTTGCAATTCTTGAAGCATATGCTCTTTATGACCCTGAAATTGGTTATTGTCAAGGCATGAGCGATCTACTCTCTCCTATCATTACTGTAATTGCTGAGGATCATGAGGCATTCTGGTGTTTTGTTGGCTTTATGCGCAAGGCTCGCCACAATTTTAGGCTCGATGAGGTTGGAATTAGAAGGCAGTTAAACATAGTCTCTAAGATTATTAGGTGCAAGGACTCGCACCTTTACAAGCACCTGGAGAAACTTCAGGCTGAGGATTGCTTTTTCGTATACCGGATGGTTGTGGTGCTATTCAGAAGGGAATTGACTTTTGAACAGACGTTATGTCTTTGGGAAGTCATATGGGCAGATCAGGCAGCCATAAGGGCTGGAATAGGGAAGTCTGCATGGAGCCGAATAAGGCAACGAGCTCCGCCAACAGATGATTTATTGCTTTACGCGATTGCTGCTTCGGTATTGCAGAGAAGGAAACTGATCATAGAGAAGTATAGTAGCATGGATGAAATTCTGAGGGAATGTAACAGCATGGCTGGACACCTTGATGTGTGGAAGCTTCTGGATGGTGCGCACAATTTGGTGGTAACCTTACACGACAAGATAGAGACGTCCTTC

mRNA sequence

AGGTGTCGTCTCAAATCACCATGGTCGCGCAGAAAAAGAAAAAATGCACTTTCATCTCGACAATGGAGAACTGTGTTTAATCCAGATGGGAAACTCCGGGATGGTGGAATTAAGTTTGTAAAAAAAGTTCGCAGTGGAGGTGTTGATCCAAGTATTCGGGCAGAGGTTTGGCCTTTCCTTCTTGGAGTCTATGACCTCAAGAGTTCTAAAGAGGAAAGGGATATTATAAAGACTCAAAAAAGAAAGGAATATGAAAAACTCCGTAAACAGTGCAGGCGATTGATAAAACGTAGGAATGAAAGCTCTAAATGGAATGAATTTGGGGACATGATTGTCGGGGAAGATGGCTTTCGCTTTCTTGTGCGAGACATAGATTCTCCTAGTTCTGAAGATGTGGTTAGTGCTAGAGAGTCTCTTTCGAGTGAAGAAAGGTGCTCAAATGTTGAATTTTTGGATGAACCCTTCTGCTCTTTGTTGGAAGGGGAGGGGAGTTCAAGACAGATCGCAGCTGATGGTTCTTTGGTACTGAATTCGGACTCCTCTGACTCAGACTCCTCAGACGACTTGGATGTTAGTCAGACTTTTCCCCCCACAGATGGAATGGAAGAACCTTATCTTGATATGACGCCTAAGGAAAATTCTTCTCCCCCTAGGACTGAGGTCTCATCAAAGCTCCATAATAGTGAAGACTTTGCAACATGGCAGCGGATTATCCGACTTGATGCACTTCGTTCTAACTCAGAATGGGTACCATACTTGACATCTCAAGCAATGGTTTCGGATGGCAGAGCCCGGCGGTCTGCTGAGGCTGTTGGTTTAGTTGATTATGACCATCTAGAACCTTGTATGATCTTCCATGCTGCAAGACTAGTTGCAATTCTTGAAGCATATGCTCTTTATGACCCTGAAATTGGTTATTGTCAAGGCATGAGCGATCTACTCTCTCCTATCATTACTGTAATTGCTGAGGATCATGAGGCATTCTGGTGTTTTGTTGGCTTTATGCGCAAGGCTCGCCACAATTTTAGGCTCGATGAGGTTGGAATTAGAAGGCAGTTAAACATAGTCTCTAAGATTATTAGGTGCAAGGACTCGCACCTTTACAAGCACCTGGAGAAACTTCAGGCTGAGGATTGCTTTTTCGTATACCGGATGGTTGTGGTGCTATTCAGAAGGGAATTGACTTTTGAACAGACGTTATGTCTTTGGGAAGTCATATGGGCAGATCAGGCAGCCATAAGGGCTGGAATAGGGAAGTCTGCATGGAGCCGAATAAGGCAACGAGCTCCGCCAACAGATGATTTATTGCTTTACGCGATTGCTGCTTCGGTATTGCAGAGAAGGAAACTGATCATAGAGAAGTATAGTAGCATGGATGAAATTCTGAGGGAATGTAACAGCATGGCTGGACACCTTGATGTGTGGAAGCTTCTGGATGGTGCGCACAATTTGGTGGTAACCTTACACGACAAGATAGAGACGTCCTTC

Coding sequence (CDS)

AGGTGTCGTCTCAAATCACCATGGTCGCGCAGAAAAAGAAAAAATGCACTTTCATCTCGACAATGGAGAACTGTGTTTAATCCAGATGGGAAACTCCGGGATGGTGGAATTAAGTTTGTAAAAAAAGTTCGCAGTGGAGGTGTTGATCCAAGTATTCGGGCAGAGGTTTGGCCTTTCCTTCTTGGAGTCTATGACCTCAAGAGTTCTAAAGAGGAAAGGGATATTATAAAGACTCAAAAAAGAAAGGAATATGAAAAACTCCGTAAACAGTGCAGGCGATTGATAAAACGTAGGAATGAAAGCTCTAAATGGAATGAATTTGGGGACATGATTGTCGGGGAAGATGGCTTTCGCTTTCTTGTGCGAGACATAGATTCTCCTAGTTCTGAAGATGTGGTTAGTGCTAGAGAGTCTCTTTCGAGTGAAGAAAGGTGCTCAAATGTTGAATTTTTGGATGAACCCTTCTGCTCTTTGTTGGAAGGGGAGGGGAGTTCAAGACAGATCGCAGCTGATGGTTCTTTGGTACTGAATTCGGACTCCTCTGACTCAGACTCCTCAGACGACTTGGATGTTAGTCAGACTTTTCCCCCCACAGATGGAATGGAAGAACCTTATCTTGATATGACGCCTAAGGAAAATTCTTCTCCCCCTAGGACTGAGGTCTCATCAAAGCTCCATAATAGTGAAGACTTTGCAACATGGCAGCGGATTATCCGACTTGATGCACTTCGTTCTAACTCAGAATGGGTACCATACTTGACATCTCAAGCAATGGTTTCGGATGGCAGAGCCCGGCGGTCTGCTGAGGCTGTTGGTTTAGTTGATTATGACCATCTAGAACCTTGTATGATCTTCCATGCTGCAAGACTAGTTGCAATTCTTGAAGCATATGCTCTTTATGACCCTGAAATTGGTTATTGTCAAGGCATGAGCGATCTACTCTCTCCTATCATTACTGTAATTGCTGAGGATCATGAGGCATTCTGGTGTTTTGTTGGCTTTATGCGCAAGGCTCGCCACAATTTTAGGCTCGATGAGGTTGGAATTAGAAGGCAGTTAAACATAGTCTCTAAGATTATTAGGTGCAAGGACTCGCACCTTTACAAGCACCTGGAGAAACTTCAGGCTGAGGATTGCTTTTTCGTATACCGGATGGTTGTGGTGCTATTCAGAAGGGAATTGACTTTTGAACAGACGTTATGTCTTTGGGAAGTCATATGGGCAGATCAGGCAGCCATAAGGGCTGGAATAGGGAAGTCTGCATGGAGCCGAATAAGGCAACGAGCTCCGCCAACAGATGATTTATTGCTTTACGCGATTGCTGCTTCGGTATTGCAGAGAAGGAAACTGATCATAGAGAAGTATAGTAGCATGGATGAAATTCTGAGGGAATGTAACAGCATGGCTGGACACCTTGATGTGTGGAAGCTTCTGGATGGTGCGCACAATTTGGTGGTAACCTTACACGACAAGATAGAGACGTCCTTC

Protein sequence

RCRLKSPWSRRKRKNALSSRQWRTVFNPDGKLRDGGIKFVKKVRSGGVDPSIRAEVWPFLLGVYDLKSSKEERDIIKTQKRKEYEKLRKQCRRLIKRRNESSKWNEFGDMIVGEDGFRFLVRDIDSPSSEDVVSARESLSSEERCSNVEFLDEPFCSLLEGEGSSRQIAADGSLVLNSDSSDSDSSDDLDVSQTFPPTDGMEEPYLDMTPKENSSPPRTEVSSKLHNSEDFATWQRIIRLDALRSNSEWVPYLTSQAMVSDGRARRSAEAVGLVDYDHLEPCMIFHAARLVAILEAYALYDPEIGYCQGMSDLLSPIITVIAEDHEAFWCFVGFMRKARHNFRLDEVGIRRQLNIVSKIIRCKDSHLYKHLEKLQAEDCFFVYRMVVVLFRRELTFEQTLCLWEVIWADQAAIRAGIGKSAWSRIRQRAPPTDDLLLYAIAASVLQRRKLIIEKYSSMDEILRECNSMAGHLDVWKLLDGAHNLVVTLHDKIETSF
Homology
BLAST of MS006173 vs. NCBI nr
Match: XP_022137806.1 (GTPase-activating protein gyp7-like [Momordica charantia])

HSP 1 Score: 977.2 bits (2525), Expect = 5.1e-281
Identity = 493/496 (99.40%), Postives = 494/496 (99.60%), Query Frame = 0

Query: 1   RCRLKSPWSRRKRKNALSSRQWRTVFNPDGKLRDGGIKFVKKVRSGGVDPSIRAEVWPFL 60
           RCRLKSPWSRRKRKNALSSRQWRTVFNPDGKLRDGGIKFVKKVRSGGVDPSIRAEVWPFL
Sbjct: 51  RCRLKSPWSRRKRKNALSSRQWRTVFNPDGKLRDGGIKFVKKVRSGGVDPSIRAEVWPFL 110

Query: 61  LGVYDLKSSKEERDIIKTQKRKEYEKLRKQCRRLIKRRNESSKWNEFGDMIVGEDGFRFL 120
           LGVYDLKSSKEERDIIKTQKRKEYEKLRKQCRRLIKRRNESSKWNEFGDMIVGEDG  FL
Sbjct: 111 LGVYDLKSSKEERDIIKTQKRKEYEKLRKQCRRLIKRRNESSKWNEFGDMIVGEDG--FL 170

Query: 121 VRDIDSPSSEDVVSARESLSSEERCSNVEFLDEPFCSLLEGEGSSRQIAADGSLVLNSDS 180
           VRDIDSPSSEDVVSARESLSSEERCSNVEFLDEPFCSLLEGEGSSRQIAADGSLVLNSDS
Sbjct: 171 VRDIDSPSSEDVVSARESLSSEERCSNVEFLDEPFCSLLEGEGSSRQIAADGSLVLNSDS 230

Query: 181 SDSDSSDDLDVSQTFPPTDGMEEPYLDMTPKENSSPPRTEVSSKLHNSEDFATWQRIIRL 240
           SDSDSSDDLDVSQTFPPTDGMEEPYLDMTPKENSSPPRTEVSSKLHNSEDFATWQRIIRL
Sbjct: 231 SDSDSSDDLDVSQTFPPTDGMEEPYLDMTPKENSSPPRTEVSSKLHNSEDFATWQRIIRL 290

Query: 241 DALRSNSEWVPYLTSQAMVSDGRARRSAEAVGLVDYDHLEPCMIFHAARLVAILEAYALY 300
           DALRSNSEWVPYLTSQAMVSDGRARRSAEAVGLVDYDHLEPCMIFHAARLVAILEAYALY
Sbjct: 291 DALRSNSEWVPYLTSQAMVSDGRARRSAEAVGLVDYDHLEPCMIFHAARLVAILEAYALY 350

Query: 301 DPEIGYCQGMSDLLSPIITVIAEDHEAFWCFVGFMRKARHNFRLDEVGIRRQLNIVSKII 360
           DPEIGYCQGMSDLLSPIITVIAEDHEAFWCFVGFMRKARHNFRLDEVGIRRQLNIV+KII
Sbjct: 351 DPEIGYCQGMSDLLSPIITVIAEDHEAFWCFVGFMRKARHNFRLDEVGIRRQLNIVAKII 410

Query: 361 RCKDSHLYKHLEKLQAEDCFFVYRMVVVLFRRELTFEQTLCLWEVIWADQAAIRAGIGKS 420
           RCKDSHLYKHLEKLQAEDCFFVYRMVVVLFRRELTFEQTLCLWEVIWADQAAIRAGIGKS
Sbjct: 411 RCKDSHLYKHLEKLQAEDCFFVYRMVVVLFRRELTFEQTLCLWEVIWADQAAIRAGIGKS 470

Query: 421 AWSRIRQRAPPTDDLLLYAIAASVLQRRKLIIEKYSSMDEILRECNSMAGHLDVWKLLDG 480
           AWSRIRQRAPPTDDLLLYAIAASVLQRRKLIIEKYSSMDEILRECNSMAGHLDVWKLLDG
Sbjct: 471 AWSRIRQRAPPTDDLLLYAIAASVLQRRKLIIEKYSSMDEILRECNSMAGHLDVWKLLDG 530

Query: 481 AHNLVVTLHDKIETSF 497
           AHNLVVTLHDKIETSF
Sbjct: 531 AHNLVVTLHDKIETSF 544

BLAST of MS006173 vs. NCBI nr
Match: XP_008437644.1 (PREDICTED: GTPase-activating protein gyp7-like [Cucumis melo] >KAA0042529.1 GTPase-activating protein gyp7-like [Cucumis melo var. makuwa] >TYK05932.1 GTPase-activating protein gyp7-like [Cucumis melo var. makuwa])

HSP 1 Score: 926.0 bits (2392), Expect = 1.4e-265
Identity = 470/497 (94.57%), Postives = 483/497 (97.18%), Query Frame = 0

Query: 1   RCRLKSPWSRRKRKNALSSRQWRTVFNPDGKLRDGGIKFVKKVRSGGVDPSIRAEVWPFL 60
           R RLKSPWSRRKRK+ALS RQW+TVF+PDGKLRDGGIKF+KKVRSGGVDPSIRAEVWPFL
Sbjct: 55  RSRLKSPWSRRKRKHALSPRQWKTVFSPDGKLRDGGIKFLKKVRSGGVDPSIRAEVWPFL 114

Query: 61  LGVYDLKSSKEERDIIKTQKRKEYEKLRKQCRRLIKRRNESSKWNEFGDMI-VGEDGFRF 120
           LGVYDLKSSK+ERDIIKTQKRKEYEKLRKQCRRLIKRRNESSKWNEF DMI VGEDG  F
Sbjct: 115 LGVYDLKSSKKERDIIKTQKRKEYEKLRKQCRRLIKRRNESSKWNEFRDMIDVGEDG--F 174

Query: 121 LVRDIDSPSSEDVVSARESLSSEERCSNVEFLDEPFCSLLEGEGSSRQIAADGSLVLNSD 180
           LV+DIDSPSSEDVVSARESLSSEERCSNVEFLDE FCSLLEGEGSSR+I AD SLVLNSD
Sbjct: 175 LVQDIDSPSSEDVVSARESLSSEERCSNVEFLDEAFCSLLEGEGSSRRITADSSLVLNSD 234

Query: 181 SSDSDSSDDLDVSQTFPPTDGMEEPYLDMTPKENSSPPRTEVSSKLHNSEDFATWQRIIR 240
           SSDSDSSDDLD+SQTFP T GMEEPYLDMTPKENSSP RTEVSSKLHNSEDFATWQRIIR
Sbjct: 235 SSDSDSSDDLDLSQTFPSTGGMEEPYLDMTPKENSSPSRTEVSSKLHNSEDFATWQRIIR 294

Query: 241 LDALRSNSEWVPYLTSQAMVSDGRARRSAEAVGLVDYDHLEPCMIFHAARLVAILEAYAL 300
           LDALRSNSEWVPYL+SQA VSDGRARR AEAVGLVDYDHLEPCMIFHAARLVAILEAYAL
Sbjct: 295 LDALRSNSEWVPYLSSQATVSDGRARRCAEAVGLVDYDHLEPCMIFHAARLVAILEAYAL 354

Query: 301 YDPEIGYCQGMSDLLSPIITVIAEDHEAFWCFVGFMRKARHNFRLDEVGIRRQLNIVSKI 360
           YDPEIGYCQGMSDLLSPIITVI+EDHEAFWCFVGFMRKARHNFRLDEVGIRRQLNIVSKI
Sbjct: 355 YDPEIGYCQGMSDLLSPIITVISEDHEAFWCFVGFMRKARHNFRLDEVGIRRQLNIVSKI 414

Query: 361 IRCKDSHLYKHLEKLQAEDCFFVYRMVVVLFRRELTFEQTLCLWEVIWADQAAIRAGIGK 420
           IRCKDSHLYKHLEKL+AEDCFFVYRMVVVLFRRELTFEQTLCLWEVIWADQAAIRAGIGK
Sbjct: 415 IRCKDSHLYKHLEKLEAEDCFFVYRMVVVLFRRELTFEQTLCLWEVIWADQAAIRAGIGK 474

Query: 421 SAWSRIRQRAPPTDDLLLYAIAASVLQRRKLIIEKYSSMDEILRECNSMAGHLDVWKLLD 480
           SAWSRIRQRAPPTDDLLLYAIAASVLQRRKLIIEKY+SMDEILRECNSMAGHLDVWKLLD
Sbjct: 475 SAWSRIRQRAPPTDDLLLYAIAASVLQRRKLIIEKYNSMDEILRECNSMAGHLDVWKLLD 534

Query: 481 GAHNLVVTLHDKIETSF 497
           GAH+LVVTLHDKIETSF
Sbjct: 535 GAHDLVVTLHDKIETSF 549

BLAST of MS006173 vs. NCBI nr
Match: XP_004145946.2 (GTPase-activating protein gyp7 [Cucumis sativus] >KGN49836.1 hypothetical protein Csa_000083 [Cucumis sativus])

HSP 1 Score: 923.7 bits (2386), Expect = 6.7e-265
Identity = 469/497 (94.37%), Postives = 483/497 (97.18%), Query Frame = 0

Query: 1   RCRLKSPWSRRKRKNALSSRQWRTVFNPDGKLRDGGIKFVKKVRSGGVDPSIRAEVWPFL 60
           R RLKSPWSRRKRK+ALS RQW+TVF+PDGKLRDGGIKF+KKVRSGGVDPSIRAEVWPFL
Sbjct: 63  RSRLKSPWSRRKRKHALSPRQWKTVFSPDGKLRDGGIKFLKKVRSGGVDPSIRAEVWPFL 122

Query: 61  LGVYDLKSSKEERDIIKTQKRKEYEKLRKQCRRLIKRRNESSKWNEFGDMI-VGEDGFRF 120
           LGVYDLKSSK+ERDIIKTQKRKEYEKLRKQCRRLIKRRNESSKWNEF DMI VGEDG  F
Sbjct: 123 LGVYDLKSSKKERDIIKTQKRKEYEKLRKQCRRLIKRRNESSKWNEFRDMIDVGEDG--F 182

Query: 121 LVRDIDSPSSEDVVSARESLSSEERCSNVEFLDEPFCSLLEGEGSSRQIAADGSLVLNSD 180
           LV+DIDSPSSEDVVSARESLSSEERCSNVEFLDE FCSLLEGEGSSR+I AD SLVLNSD
Sbjct: 183 LVQDIDSPSSEDVVSARESLSSEERCSNVEFLDEAFCSLLEGEGSSRRITADSSLVLNSD 242

Query: 181 SSDSDSSDDLDVSQTFPPTDGMEEPYLDMTPKENSSPPRTEVSSKLHNSEDFATWQRIIR 240
           SSDSDSSDDLD+SQTFP TDGMEEPYLDMT KENSSP RTEVSSKLHNSEDFATWQRIIR
Sbjct: 243 SSDSDSSDDLDLSQTFPSTDGMEEPYLDMTAKENSSPTRTEVSSKLHNSEDFATWQRIIR 302

Query: 241 LDALRSNSEWVPYLTSQAMVSDGRARRSAEAVGLVDYDHLEPCMIFHAARLVAILEAYAL 300
           LDALRSNSEWVPYL+SQA VSDGRARR AEAVGLVDYDHLEPCMIFHAARLVAILEAYAL
Sbjct: 303 LDALRSNSEWVPYLSSQAPVSDGRARRCAEAVGLVDYDHLEPCMIFHAARLVAILEAYAL 362

Query: 301 YDPEIGYCQGMSDLLSPIITVIAEDHEAFWCFVGFMRKARHNFRLDEVGIRRQLNIVSKI 360
           +DPEIGYCQGMSDLLSPIITVI+EDHEAFWCFVGFMRKARHNFRLDEVGIRRQLNIVSKI
Sbjct: 363 FDPEIGYCQGMSDLLSPIITVISEDHEAFWCFVGFMRKARHNFRLDEVGIRRQLNIVSKI 422

Query: 361 IRCKDSHLYKHLEKLQAEDCFFVYRMVVVLFRRELTFEQTLCLWEVIWADQAAIRAGIGK 420
           IRCKDSHLYKHLEKL+AEDCFFVYRMVVVLFRRELTFEQTLCLWEVIWADQAAIRAGIGK
Sbjct: 423 IRCKDSHLYKHLEKLEAEDCFFVYRMVVVLFRRELTFEQTLCLWEVIWADQAAIRAGIGK 482

Query: 421 SAWSRIRQRAPPTDDLLLYAIAASVLQRRKLIIEKYSSMDEILRECNSMAGHLDVWKLLD 480
           SAWSRIRQRAPPTDDLLLYAIAASVLQRRKLIIEKY+SMDEILRECNSMAGHLDVWKLLD
Sbjct: 483 SAWSRIRQRAPPTDDLLLYAIAASVLQRRKLIIEKYNSMDEILRECNSMAGHLDVWKLLD 542

Query: 481 GAHNLVVTLHDKIETSF 497
           GAH+LVVTLHDKIETSF
Sbjct: 543 GAHDLVVTLHDKIETSF 557

BLAST of MS006173 vs. NCBI nr
Match: XP_038875646.1 (GTPase-activating protein gyp7-like [Benincasa hispida])

HSP 1 Score: 914.1 bits (2361), Expect = 5.3e-262
Identity = 466/497 (93.76%), Postives = 478/497 (96.18%), Query Frame = 0

Query: 1   RCRLKSPWSRRKRKNALSSRQWRTVFNPDGKLRDGGIKFVKKVRSGGVDPSIRAEVWPFL 60
           R RLKSPWSRRKRK+ALS RQW+TVFNPDGKLRDGGIKF+KKVRSGGVDPSIRAEVWPFL
Sbjct: 55  RSRLKSPWSRRKRKHALSPRQWKTVFNPDGKLRDGGIKFLKKVRSGGVDPSIRAEVWPFL 114

Query: 61  LGVYDLKSSKEERDIIKTQKRKEYEKLRKQCRRLIKRRNESSKWNEFGDMI-VGEDGFRF 120
           LGVYDLKSSK+ERDIIKTQKRKEYEKLRKQCRRLIKRRNESSKWNEF DMI VGEDG  F
Sbjct: 115 LGVYDLKSSKKERDIIKTQKRKEYEKLRKQCRRLIKRRNESSKWNEFRDMIDVGEDG--F 174

Query: 121 LVRDIDSPSSEDVVSARESLSSEERCSNVEFLDEPFCSLLEGEGSSRQIAADGSLVLNSD 180
           LVRDIDSPSSEDVVSARESLSSEERCSNVE LDE FCSLLEGEGSSR+I AD SLVLNSD
Sbjct: 175 LVRDIDSPSSEDVVSARESLSSEERCSNVELLDEAFCSLLEGEGSSRRITADSSLVLNSD 234

Query: 181 SSDSDSSDDLDVSQTFPPTDGMEEPYLDMTPKENSSPPRTEVSSKLHNSEDFATWQRIIR 240
            SDSDSSDDLD+SQTFP  DGMEEPYLDMT KENSSP  T+VSSKLHNSEDFATWQRIIR
Sbjct: 235 FSDSDSSDDLDLSQTFPSMDGMEEPYLDMTLKENSSPSSTDVSSKLHNSEDFATWQRIIR 294

Query: 241 LDALRSNSEWVPYLTSQAMVSDGRARRSAEAVGLVDYDHLEPCMIFHAARLVAILEAYAL 300
           LDALRSNSEWVPYL+SQA VSDGRARR AEAVGLVDYDHLEPCMIFHAARLVAILEAYAL
Sbjct: 295 LDALRSNSEWVPYLSSQATVSDGRARRCAEAVGLVDYDHLEPCMIFHAARLVAILEAYAL 354

Query: 301 YDPEIGYCQGMSDLLSPIITVIAEDHEAFWCFVGFMRKARHNFRLDEVGIRRQLNIVSKI 360
           YDPEIGYCQGMSDLLSPIITVI+EDHEAFWCFVGFMRKARHNFRLDEVGIRRQLNIVSKI
Sbjct: 355 YDPEIGYCQGMSDLLSPIITVISEDHEAFWCFVGFMRKARHNFRLDEVGIRRQLNIVSKI 414

Query: 361 IRCKDSHLYKHLEKLQAEDCFFVYRMVVVLFRRELTFEQTLCLWEVIWADQAAIRAGIGK 420
           IR KDSHLYKHLEKL+AEDCFFVYRMVVVLFRRELTFEQTLCLWEVIWADQAAIRAGIGK
Sbjct: 415 IRSKDSHLYKHLEKLEAEDCFFVYRMVVVLFRRELTFEQTLCLWEVIWADQAAIRAGIGK 474

Query: 421 SAWSRIRQRAPPTDDLLLYAIAASVLQRRKLIIEKYSSMDEILRECNSMAGHLDVWKLLD 480
           SAWSRIRQRAPPTDDLLLYAIAASVLQRRKLIIEKY+SMDEILRECNSMAGHLDVWKLLD
Sbjct: 475 SAWSRIRQRAPPTDDLLLYAIAASVLQRRKLIIEKYNSMDEILRECNSMAGHLDVWKLLD 534

Query: 481 GAHNLVVTLHDKIETSF 497
           GAH+LVVTLHDKIETSF
Sbjct: 535 GAHDLVVTLHDKIETSF 549

BLAST of MS006173 vs. NCBI nr
Match: XP_022995580.1 (GTPase-activating protein gyp7-like [Cucurbita maxima])

HSP 1 Score: 904.8 bits (2337), Expect = 3.2e-259
Identity = 461/498 (92.57%), Postives = 477/498 (95.78%), Query Frame = 0

Query: 1   RCRLKSPWSRRKRKNALSSRQWRTVFNPDGKLRDGGIKFVKKVRSGGVDPSIRAEVWPFL 60
           R RLKSPWSRRKRK+ALS RQW+TVFN DGKLRD GIKF+KKVRSGGVD SIRAEVWPFL
Sbjct: 49  RTRLKSPWSRRKRKHALSPRQWKTVFNLDGKLRDSGIKFLKKVRSGGVDSSIRAEVWPFL 108

Query: 61  LGVYDLKSSKEERDIIKTQKRKEYEKLRKQCRRLIKRRNESSKWNEFGDMI-VGEDGFRF 120
           LGVYDLKSSKEERDIIKTQKRKEYEKLRK+CRRLIKRRNESSKWNEFGDMI VGEDG  F
Sbjct: 109 LGVYDLKSSKEERDIIKTQKRKEYEKLRKRCRRLIKRRNESSKWNEFGDMIDVGEDG--F 168

Query: 121 LVRDIDSPSSEDVVSARESLSSEERCSNVEFLDEPFCSLLEGEGSSRQIAADGSLVLNSD 180
           LVRD+DSPSSEDVVSARESLSSEERCSNV+FLDEPF SLLEGEGSSR+I ADGSLVLNSD
Sbjct: 169 LVRDVDSPSSEDVVSARESLSSEERCSNVDFLDEPFNSLLEGEGSSRRITADGSLVLNSD 228

Query: 181 SSDSDSSDDLDVSQTFPPTDGMEEPYLDMTPKENSSPPRTEV-SSKLHNSEDFATWQRII 240
           SSDS+SSDDLDVSQ FP TDGMEEP+LD  PKENSSP R E+ SSKLH+ EDFATWQRII
Sbjct: 229 SSDSESSDDLDVSQAFPSTDGMEEPFLDSMPKENSSPSRAEISSSKLHSGEDFATWQRII 288

Query: 241 RLDALRSNSEWVPYLTSQAMVSDGRARRSAEAVGLVDYDHLEPCMIFHAARLVAILEAYA 300
           RLDALRSNSEWVPYL+SQAMVSDGRARR AEAVGLVDYDHLEPCMIFHAARLVAILEAYA
Sbjct: 289 RLDALRSNSEWVPYLSSQAMVSDGRARRCAEAVGLVDYDHLEPCMIFHAARLVAILEAYA 348

Query: 301 LYDPEIGYCQGMSDLLSPIITVIAEDHEAFWCFVGFMRKARHNFRLDEVGIRRQLNIVSK 360
           LYDPEIGYCQGMSDLLSPIITVI EDHEAFWCFVGFMRKARHNFRLDEVGIRRQL+IVSK
Sbjct: 349 LYDPEIGYCQGMSDLLSPIITVIPEDHEAFWCFVGFMRKARHNFRLDEVGIRRQLSIVSK 408

Query: 361 IIRCKDSHLYKHLEKLQAEDCFFVYRMVVVLFRRELTFEQTLCLWEVIWADQAAIRAGIG 420
           II+CKD HLYKHLEKLQAEDCFFVYRMVVVLFRRELTFEQTLCLWEVIWADQAAIRAG+G
Sbjct: 409 IIKCKDPHLYKHLEKLQAEDCFFVYRMVVVLFRRELTFEQTLCLWEVIWADQAAIRAGMG 468

Query: 421 KSAWSRIRQRAPPTDDLLLYAIAASVLQRRKLIIEKYSSMDEILRECNSMAGHLDVWKLL 480
           KSAWSRIRQRAPPTDDLLLYAIAASVLQRRKLIIEKY+SMDEILRECNSMAGHLDVWKLL
Sbjct: 469 KSAWSRIRQRAPPTDDLLLYAIAASVLQRRKLIIEKYNSMDEILRECNSMAGHLDVWKLL 528

Query: 481 DGAHNLVVTLHDKIETSF 497
           DGAHNLVVTLHDKIETSF
Sbjct: 529 DGAHNLVVTLHDKIETSF 544

BLAST of MS006173 vs. ExPASy Swiss-Prot
Match: Q94BY9 (Rab GTPase-activating protein 22 OS=Arabidopsis thaliana OX=3702 GN=RABGAP22 PE=1 SV=1)

HSP 1 Score: 574.3 bits (1479), Expect = 1.3e-162
Identity = 311/500 (62.20%), Postives = 374/500 (74.80%), Query Frame = 0

Query: 3   RLKSPWSRRKRKNALSSRQWRTVFNPDGKLRDGGIKFVKKVRSGGVDPSIRAEVWPFLLG 62
           R KSPWSRRKRK AL+  QWR++F P+GKLRDGG+ F+KKVRS GVDPSIRAEVW FLLG
Sbjct: 82  RRKSPWSRRKRKWALTPHQWRSLFTPEGKLRDGGVGFLKKVRSRGVDPSIRAEVWLFLLG 141

Query: 63  VYDLKSSKEERDIIKTQKRKEYEKLRKQCRRLIKRRNESSKWNEFGDMIVGEDGFRFLVR 122
           VYDL S+ EER+ +KTQKRKEYEKL+++C+ L+K  N S+                    
Sbjct: 142 VYDLNSTSEEREAVKTQKRKEYEKLQRRCQMLLKCGNGSTD------------------- 201

Query: 123 DIDSPSSEDVVSARESLSSEERCSNVEFLDEPFCSLLEGEGSSRQIAADGSLVLNSDSSD 182
                      +  E  S E     V F+D+     + G  +S+ + +     LN+DSSD
Sbjct: 202 -----------NLEELPSDEANSQCVRFVDD---YKITGPMTSQDVVS----ALNTDSSD 261

Query: 183 SDS---SDDLDVSQTFPPTDGM--EEPYLDMTPKENSS---PPRTEVSSKLHNSEDFATW 242
           +DS   ++D+ +  +F  +D    EE   +   +ENSS      +EV  ++   EDF+TW
Sbjct: 262 TDSCEDNEDVLLLSSFAHSDEKKPEEDNSNNNSEENSSLLVAAASEVQVEVAVHEDFSTW 321

Query: 243 QRIIRLDALRSNSEWVPYLTSQAMVSDGRARRSAEAVGLVDYDHLEPCMIFHAARLVAIL 302
           QRIIRLDALR++SEW  Y      +++ +ARR AE+VGL DYDHLE C ++HAARLVAIL
Sbjct: 322 QRIIRLDALRADSEWANYSPYSTAITESKARRLAESVGLKDYDHLESCRLYHAARLVAIL 381

Query: 303 EAYALYDPEIGYCQGMSDLLSPIITVIAEDHEAFWCFVGFMRKARHNFRLDEVGIRRQLN 362
           EAYA+YDPEIGYCQGMSDLLSPI+ VI+EDHEAFWCFVGFM+KARHNFRLDE GI+RQL+
Sbjct: 382 EAYAMYDPEIGYCQGMSDLLSPILAVISEDHEAFWCFVGFMKKARHNFRLDEAGIQRQLS 441

Query: 363 IVSKIIRCKDSHLYKHLEKLQAEDCFFVYRMVVVLFRRELTFEQTLCLWEVIWADQAAIR 422
           IVSKII+ KDS LYKHLE LQAEDC FVYRMV+V+FRREL+FEQTLCLWEV+WADQAAIR
Sbjct: 442 IVSKIIKNKDSQLYKHLENLQAEDCSFVYRMVLVMFRRELSFEQTLCLWEVMWADQAAIR 501

Query: 423 AGIGKSAWSRIRQRAPPTDDLLLYAIAASVLQRRKLIIEKYSSMDEILRECNSMAGHLDV 482
           AG+GKS WSRIRQ+APPTDDLLLYAIAA VL RRKLII+KYSSMDEI+ ECNSMAG L+V
Sbjct: 502 AGVGKSPWSRIRQQAPPTDDLLLYAIAALVL-RRKLIIQKYSSMDEIVEECNSMAGQLNV 543

Query: 483 WKLLDGAHNLVVTLHDKIET 495
           WKLLD AH+LVVTLHDKIET
Sbjct: 562 WKLLDDAHHLVVTLHDKIET 543

BLAST of MS006173 vs. ExPASy Swiss-Prot
Match: P09379 (GTPase-activating protein GYP7 OS=Yarrowia lipolytica (strain CLIB 122 / E 150) OX=284591 GN=GYP7 PE=3 SV=2)

HSP 1 Score: 121.7 bits (304), Expect = 2.3e-26
Identity = 66/208 (31.73%), Postives = 109/208 (52.40%), Query Frame = 0

Query: 286 HAARLVAILEAYALYDPEIGYCQGMSDLLSPIITVIAEDHEAFWCFVGFMRKARHNFRLD 345
           H  +L  +L  Y  ++  +GY QGMSDLLSP+  V+ +D  AFW F  FM +   N+  D
Sbjct: 495 HLIQLRDMLITYNQHNKNLGYVQGMSDLLSPLYVVLQDDTLAFWAFSAFMERMERNYLRD 554

Query: 346 EVGIRRQLNIVSKIIRCKDSHLYKHLEKLQAEDCFFVYRMVVVLFRRELTFEQTLCLWEV 405
           + G+R QL  +  +++     LYKHLEK ++ + FF +RM++V F+REL ++  L LWEV
Sbjct: 555 QSGMRNQLLCLDHLVQFMLPSLYKHLEKTESTNLFFFFRMLLVWFKRELLWDDVLRLWEV 614

Query: 406 IWADQAAIRAGIGKSAWSRIRQRAPPTDDLLLYAIAASVLQRRKLIIEKYSSMDEILREC 465
           +W D                      +   +L+   A + + + ++I+  +  DEIL+  
Sbjct: 615 LWTDYL--------------------SSQFVLFVCLAILDKHKDVMIDHLAGFDEILKYM 674

Query: 466 NSMAGHLDVWKLLDGAHNLVVTLHDKIE 494
           N ++  +D+ +LL  A  L       +E
Sbjct: 675 NELSMTIDLDELLVRAELLFYRFRRTVE 682


HSP 2 Score: 50.4 bits (119), Expect = 6.6e-05
Identity = 26/79 (32.91%), Postives = 48/79 (60.76%), Query Frame = 0

Query: 12  KRKNALSSRQWRTVFNPDGKLRDGGIKFVKKVRSGGVDPSIRAEVWPFLLGVYDLKSSKE 71
           +R+N +S  +W   F+ +G+L     +  +++  GG+ P++R E W FLLGVY   S+  
Sbjct: 353 QRRNEVSLAEWNAFFDYNGRLIVTVNEVKERIFHGGLAPAVRPEGWLFLLGVYPWDSTAA 412

Query: 72  ERDIIKTQKRKEYEKLRKQ 91
           ER  + ++ R +Y +L+K+
Sbjct: 413 ERKELVSKLRVDYNRLKKE 431

BLAST of MS006173 vs. ExPASy Swiss-Prot
Match: Q9UUH7 (GTPase-activating protein gyp7 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=gyp7 PE=3 SV=1)

HSP 1 Score: 112.8 bits (281), Expect = 1.1e-23
Identity = 109/468 (23.29%), Postives = 171/468 (36.54%), Query Frame = 0

Query: 10  RRKRKNALSSRQWRTVFNPDGKLRDGGIKFVKKVRSGGVDPSIRAEVWPFLLGVYDLKSS 69
           R KR + LS  QW ++FN  GKL+    + +  +  GG+ PS+R EVWPFLL VY   S+
Sbjct: 374 RVKRDDPLSVEQWNSMFNAHGKLQVDVHRVLGIIFHGGIQPSLRKEVWPFLLSVYPWDST 433

Query: 70  KEERDIIKTQKRKEYEKLRKQCRRLIKRRNESSKWNEFGDMIVGEDGFRFLVRDIDSPSS 129
            EER +I    ++EY  L++             KW E                       
Sbjct: 434 SEERRVIYLSLQEEYCTLKR-------------KWYE----------------------- 493

Query: 130 EDVVSARESLSSEERCSNVEFLDEPFCSLLEGEGSSRQIAADGSLVLNSDSSDSDSSDDL 189
                                                                       
Sbjct: 494 ------------------------------------------------------------ 553

Query: 190 DVSQTFPPTDGMEEPYLDMTPKENSSPPRTEVSSKLHNSEDFATWQRIIRLDALRSNSEW 249
           D+ + F     +E+              R  +   +H                       
Sbjct: 554 DIHKQFNDRWFIEQ--------------RNRIEKDVH----------------------- 613

Query: 250 VPYLTSQAMVSDGRARRSAEAVGLVDYDHLEPCMIF-----HAARLVAILEAYALYDPEI 309
                        R  R  E   + D  H +P   F     +   +  IL  Y  YD E+
Sbjct: 614 -------------RTDRQHEYFQIEDLPHPDPQSTFTGTNMNMEMMKDILLTYNEYDTEL 673

Query: 310 GYCQGMSDLLSPIITVIAEDHEAFWCFVGFMRKARHNFRLDEVGIRRQLNIVSKIIRCKD 369
           GY QGMSDLL+PI     ++   FW  VG M++   NF  D+ G+ RQL+ +  +I   D
Sbjct: 674 GYVQGMSDLLAPIYVTFNDNALTFWGMVGLMKRLHFNFLRDQSGMHRQLDTLRLLIEFMD 675

Query: 370 SHLYKHLEKLQAEDCFFVYRMVVVLFRRELTFEQTLCLWEVIWADQAAIRAGIGKSAWSR 429
             L+ HLEK  + + F  +RM+++ F+RE  +E  L LW+V++ +  +    I       
Sbjct: 734 PELFAHLEKTDSSNLFCFFRMLLIYFKREFDWEVLLKLWDVLFTNYLSYDYHI------- 675

Query: 430 IRQRAPPTDDLLLYAIAASVLQRRKLIIEKYSSMDEILRECNSMAGHL 473
                      + YAIA    + R++++ + S+ DE+L+  N ++G L
Sbjct: 794 ----------FVAYAIAE---RHREVLLNQTSAFDEVLKYFNELSGKL 675

BLAST of MS006173 vs. ExPASy Swiss-Prot
Match: Q8TC07 (TBC1 domain family member 15 OS=Homo sapiens OX=9606 GN=TBC1D15 PE=1 SV=2)

HSP 1 Score: 110.2 bits (274), Expect = 7.0e-23
Identity = 59/186 (31.72%), Postives = 97/186 (52.15%), Query Frame = 0

Query: 293 ILEAYALYDPEIGYCQGMSDLLSPIITVIAEDHEAFWCFVGFMRKARHNFRLDEVGIRRQ 352
           IL  Y +YD ++GY QGMSDLLSP++ V+  + +AFWCF  +M +   NF     G++ Q
Sbjct: 439 ILMTYCMYDFDLGYVQGMSDLLSPLLYVMENEVDAFWCFASYMDQMHQNFEEQMQGMKTQ 498

Query: 353 LNIVSKIIRCKDSHLYKHLEKLQAEDCFFVYRMVVVLFRRELTFEQTLCLWEVIWADQAA 412
           L  +S ++R  DS    +LE   +   +F +R +++ F+RE +F   L LWEV+W +   
Sbjct: 499 LIQLSTLLRLLDSGFCSYLESQDSGYLYFCFRWLLIRFKREFSFLDILRLWEVMWTEL-- 558

Query: 413 IRAGIGKSAWSRIRQRAPPTDDLLLYAIAASVLQRRKLIIEKYSSMDEILRECNSMAGHL 472
                             P  +  L    A +   ++ I+EK+   +EIL+  N ++  +
Sbjct: 559 ------------------PCTNFHLLLCCAILESEKQQIMEKHYGFNEILKHINELSMKI 604

Query: 473 DVWKLL 479
           DV  +L
Sbjct: 619 DVEDIL 604

BLAST of MS006173 vs. ExPASy Swiss-Prot
Match: Q6BU76 (GTPase-activating protein GYP7 OS=Debaryomyces hansenii (strain ATCC 36239 / CBS 767 / BCRC 21394 / JCM 1990 / NBRC 0083 / IGC 2968) OX=284592 GN=GYP7 PE=3 SV=2)

HSP 1 Score: 108.6 bits (270), Expect = 2.0e-22
Identity = 63/216 (29.17%), Postives = 112/216 (51.85%), Query Frame = 0

Query: 286 HAARLVAILEAYALYDPEIGYCQGMSDLLSPIITVIAEDHEAFWCFVGFMRKARHNFRLD 345
           H  ++  IL  Y  ++  +GY QGM+DLLSP+     ++   FW FV FM +   NF  D
Sbjct: 516 HLFKMREILLTYNEHNVNLGYVQGMTDLLSPLYVTFQDESLTFWAFVNFMDRMERNFLRD 575

Query: 346 EVGIRRQLNIVSKIIRCKDSHLYKHLEKLQAEDCFFVYRMVVVLFRRELTFEQTLCLWEV 405
           + G++ Q+  ++++++     L+KHLEK ++ D +F +RM++V F+RE  +   L LWE+
Sbjct: 576 QSGMKNQMLTLNELVQFMLPDLFKHLEKCESTDLYFFFRMLLVWFKREFEWSSVLSLWEI 635

Query: 406 IWADQAA------IRAGIGKSAWSRIRQRAPPTDDLLLYAIAAS-------VLQRRKLII 465
           +W D  +          +       IRQ     D++L Y    S       +L R +L+ 
Sbjct: 636 LWTDYYSGQFHLFFALAVLSDNERIIRQNLSRFDEVLKYMNDLSMNMNLNHLLIRAELLF 695

Query: 466 EKYSSMDEILRECNSMAGHLDVWKLLDGAHNLVVTL 489
            ++  M +I+   NS+   L+   + D A+N ++ +
Sbjct: 696 LRFRRMIDIIDRENSLK-KLNNPGVYDDANNSLIKI 730


HSP 2 Score: 47.0 bits (110), Expect = 7.3e-04
Identity = 25/80 (31.25%), Postives = 43/80 (53.75%), Query Frame = 0

Query: 9   SRRKRKNALSSRQWRTVFNPDGKLRDGGIKFVKKVRSGGVDPSIRAEVWPFLLGVYDLKS 68
           S+  R+  +S  +W  +F+  G+L     +   ++  GG++  IR E W FLL VY   S
Sbjct: 357 SKTSRRKPISKVEWEGLFDFSGRLIISIDEIKDRIFHGGLEDCIRGEAWLFLLNVYPWDS 416

Query: 69  SKEERDIIKTQKRKEYEKLR 89
           S EER  ++   +  YE+++
Sbjct: 417 SAEERKTLRNSFQTAYEEIK 436

BLAST of MS006173 vs. ExPASy TrEMBL
Match: A0A6J1C7S1 (GTPase-activating protein gyp7-like OS=Momordica charantia OX=3673 GN=LOC111009153 PE=4 SV=1)

HSP 1 Score: 977.2 bits (2525), Expect = 2.5e-281
Identity = 493/496 (99.40%), Postives = 494/496 (99.60%), Query Frame = 0

Query: 1   RCRLKSPWSRRKRKNALSSRQWRTVFNPDGKLRDGGIKFVKKVRSGGVDPSIRAEVWPFL 60
           RCRLKSPWSRRKRKNALSSRQWRTVFNPDGKLRDGGIKFVKKVRSGGVDPSIRAEVWPFL
Sbjct: 51  RCRLKSPWSRRKRKNALSSRQWRTVFNPDGKLRDGGIKFVKKVRSGGVDPSIRAEVWPFL 110

Query: 61  LGVYDLKSSKEERDIIKTQKRKEYEKLRKQCRRLIKRRNESSKWNEFGDMIVGEDGFRFL 120
           LGVYDLKSSKEERDIIKTQKRKEYEKLRKQCRRLIKRRNESSKWNEFGDMIVGEDG  FL
Sbjct: 111 LGVYDLKSSKEERDIIKTQKRKEYEKLRKQCRRLIKRRNESSKWNEFGDMIVGEDG--FL 170

Query: 121 VRDIDSPSSEDVVSARESLSSEERCSNVEFLDEPFCSLLEGEGSSRQIAADGSLVLNSDS 180
           VRDIDSPSSEDVVSARESLSSEERCSNVEFLDEPFCSLLEGEGSSRQIAADGSLVLNSDS
Sbjct: 171 VRDIDSPSSEDVVSARESLSSEERCSNVEFLDEPFCSLLEGEGSSRQIAADGSLVLNSDS 230

Query: 181 SDSDSSDDLDVSQTFPPTDGMEEPYLDMTPKENSSPPRTEVSSKLHNSEDFATWQRIIRL 240
           SDSDSSDDLDVSQTFPPTDGMEEPYLDMTPKENSSPPRTEVSSKLHNSEDFATWQRIIRL
Sbjct: 231 SDSDSSDDLDVSQTFPPTDGMEEPYLDMTPKENSSPPRTEVSSKLHNSEDFATWQRIIRL 290

Query: 241 DALRSNSEWVPYLTSQAMVSDGRARRSAEAVGLVDYDHLEPCMIFHAARLVAILEAYALY 300
           DALRSNSEWVPYLTSQAMVSDGRARRSAEAVGLVDYDHLEPCMIFHAARLVAILEAYALY
Sbjct: 291 DALRSNSEWVPYLTSQAMVSDGRARRSAEAVGLVDYDHLEPCMIFHAARLVAILEAYALY 350

Query: 301 DPEIGYCQGMSDLLSPIITVIAEDHEAFWCFVGFMRKARHNFRLDEVGIRRQLNIVSKII 360
           DPEIGYCQGMSDLLSPIITVIAEDHEAFWCFVGFMRKARHNFRLDEVGIRRQLNIV+KII
Sbjct: 351 DPEIGYCQGMSDLLSPIITVIAEDHEAFWCFVGFMRKARHNFRLDEVGIRRQLNIVAKII 410

Query: 361 RCKDSHLYKHLEKLQAEDCFFVYRMVVVLFRRELTFEQTLCLWEVIWADQAAIRAGIGKS 420
           RCKDSHLYKHLEKLQAEDCFFVYRMVVVLFRRELTFEQTLCLWEVIWADQAAIRAGIGKS
Sbjct: 411 RCKDSHLYKHLEKLQAEDCFFVYRMVVVLFRRELTFEQTLCLWEVIWADQAAIRAGIGKS 470

Query: 421 AWSRIRQRAPPTDDLLLYAIAASVLQRRKLIIEKYSSMDEILRECNSMAGHLDVWKLLDG 480
           AWSRIRQRAPPTDDLLLYAIAASVLQRRKLIIEKYSSMDEILRECNSMAGHLDVWKLLDG
Sbjct: 471 AWSRIRQRAPPTDDLLLYAIAASVLQRRKLIIEKYSSMDEILRECNSMAGHLDVWKLLDG 530

Query: 481 AHNLVVTLHDKIETSF 497
           AHNLVVTLHDKIETSF
Sbjct: 531 AHNLVVTLHDKIETSF 544

BLAST of MS006173 vs. ExPASy TrEMBL
Match: A0A5A7TMD7 (GTPase-activating protein gyp7-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold376G00240 PE=4 SV=1)

HSP 1 Score: 926.0 bits (2392), Expect = 6.6e-266
Identity = 470/497 (94.57%), Postives = 483/497 (97.18%), Query Frame = 0

Query: 1   RCRLKSPWSRRKRKNALSSRQWRTVFNPDGKLRDGGIKFVKKVRSGGVDPSIRAEVWPFL 60
           R RLKSPWSRRKRK+ALS RQW+TVF+PDGKLRDGGIKF+KKVRSGGVDPSIRAEVWPFL
Sbjct: 55  RSRLKSPWSRRKRKHALSPRQWKTVFSPDGKLRDGGIKFLKKVRSGGVDPSIRAEVWPFL 114

Query: 61  LGVYDLKSSKEERDIIKTQKRKEYEKLRKQCRRLIKRRNESSKWNEFGDMI-VGEDGFRF 120
           LGVYDLKSSK+ERDIIKTQKRKEYEKLRKQCRRLIKRRNESSKWNEF DMI VGEDG  F
Sbjct: 115 LGVYDLKSSKKERDIIKTQKRKEYEKLRKQCRRLIKRRNESSKWNEFRDMIDVGEDG--F 174

Query: 121 LVRDIDSPSSEDVVSARESLSSEERCSNVEFLDEPFCSLLEGEGSSRQIAADGSLVLNSD 180
           LV+DIDSPSSEDVVSARESLSSEERCSNVEFLDE FCSLLEGEGSSR+I AD SLVLNSD
Sbjct: 175 LVQDIDSPSSEDVVSARESLSSEERCSNVEFLDEAFCSLLEGEGSSRRITADSSLVLNSD 234

Query: 181 SSDSDSSDDLDVSQTFPPTDGMEEPYLDMTPKENSSPPRTEVSSKLHNSEDFATWQRIIR 240
           SSDSDSSDDLD+SQTFP T GMEEPYLDMTPKENSSP RTEVSSKLHNSEDFATWQRIIR
Sbjct: 235 SSDSDSSDDLDLSQTFPSTGGMEEPYLDMTPKENSSPSRTEVSSKLHNSEDFATWQRIIR 294

Query: 241 LDALRSNSEWVPYLTSQAMVSDGRARRSAEAVGLVDYDHLEPCMIFHAARLVAILEAYAL 300
           LDALRSNSEWVPYL+SQA VSDGRARR AEAVGLVDYDHLEPCMIFHAARLVAILEAYAL
Sbjct: 295 LDALRSNSEWVPYLSSQATVSDGRARRCAEAVGLVDYDHLEPCMIFHAARLVAILEAYAL 354

Query: 301 YDPEIGYCQGMSDLLSPIITVIAEDHEAFWCFVGFMRKARHNFRLDEVGIRRQLNIVSKI 360
           YDPEIGYCQGMSDLLSPIITVI+EDHEAFWCFVGFMRKARHNFRLDEVGIRRQLNIVSKI
Sbjct: 355 YDPEIGYCQGMSDLLSPIITVISEDHEAFWCFVGFMRKARHNFRLDEVGIRRQLNIVSKI 414

Query: 361 IRCKDSHLYKHLEKLQAEDCFFVYRMVVVLFRRELTFEQTLCLWEVIWADQAAIRAGIGK 420
           IRCKDSHLYKHLEKL+AEDCFFVYRMVVVLFRRELTFEQTLCLWEVIWADQAAIRAGIGK
Sbjct: 415 IRCKDSHLYKHLEKLEAEDCFFVYRMVVVLFRRELTFEQTLCLWEVIWADQAAIRAGIGK 474

Query: 421 SAWSRIRQRAPPTDDLLLYAIAASVLQRRKLIIEKYSSMDEILRECNSMAGHLDVWKLLD 480
           SAWSRIRQRAPPTDDLLLYAIAASVLQRRKLIIEKY+SMDEILRECNSMAGHLDVWKLLD
Sbjct: 475 SAWSRIRQRAPPTDDLLLYAIAASVLQRRKLIIEKYNSMDEILRECNSMAGHLDVWKLLD 534

Query: 481 GAHNLVVTLHDKIETSF 497
           GAH+LVVTLHDKIETSF
Sbjct: 535 GAHDLVVTLHDKIETSF 549

BLAST of MS006173 vs. ExPASy TrEMBL
Match: A0A1S3AUI7 (GTPase-activating protein gyp7-like OS=Cucumis melo OX=3656 GN=LOC103482988 PE=4 SV=1)

HSP 1 Score: 926.0 bits (2392), Expect = 6.6e-266
Identity = 470/497 (94.57%), Postives = 483/497 (97.18%), Query Frame = 0

Query: 1   RCRLKSPWSRRKRKNALSSRQWRTVFNPDGKLRDGGIKFVKKVRSGGVDPSIRAEVWPFL 60
           R RLKSPWSRRKRK+ALS RQW+TVF+PDGKLRDGGIKF+KKVRSGGVDPSIRAEVWPFL
Sbjct: 55  RSRLKSPWSRRKRKHALSPRQWKTVFSPDGKLRDGGIKFLKKVRSGGVDPSIRAEVWPFL 114

Query: 61  LGVYDLKSSKEERDIIKTQKRKEYEKLRKQCRRLIKRRNESSKWNEFGDMI-VGEDGFRF 120
           LGVYDLKSSK+ERDIIKTQKRKEYEKLRKQCRRLIKRRNESSKWNEF DMI VGEDG  F
Sbjct: 115 LGVYDLKSSKKERDIIKTQKRKEYEKLRKQCRRLIKRRNESSKWNEFRDMIDVGEDG--F 174

Query: 121 LVRDIDSPSSEDVVSARESLSSEERCSNVEFLDEPFCSLLEGEGSSRQIAADGSLVLNSD 180
           LV+DIDSPSSEDVVSARESLSSEERCSNVEFLDE FCSLLEGEGSSR+I AD SLVLNSD
Sbjct: 175 LVQDIDSPSSEDVVSARESLSSEERCSNVEFLDEAFCSLLEGEGSSRRITADSSLVLNSD 234

Query: 181 SSDSDSSDDLDVSQTFPPTDGMEEPYLDMTPKENSSPPRTEVSSKLHNSEDFATWQRIIR 240
           SSDSDSSDDLD+SQTFP T GMEEPYLDMTPKENSSP RTEVSSKLHNSEDFATWQRIIR
Sbjct: 235 SSDSDSSDDLDLSQTFPSTGGMEEPYLDMTPKENSSPSRTEVSSKLHNSEDFATWQRIIR 294

Query: 241 LDALRSNSEWVPYLTSQAMVSDGRARRSAEAVGLVDYDHLEPCMIFHAARLVAILEAYAL 300
           LDALRSNSEWVPYL+SQA VSDGRARR AEAVGLVDYDHLEPCMIFHAARLVAILEAYAL
Sbjct: 295 LDALRSNSEWVPYLSSQATVSDGRARRCAEAVGLVDYDHLEPCMIFHAARLVAILEAYAL 354

Query: 301 YDPEIGYCQGMSDLLSPIITVIAEDHEAFWCFVGFMRKARHNFRLDEVGIRRQLNIVSKI 360
           YDPEIGYCQGMSDLLSPIITVI+EDHEAFWCFVGFMRKARHNFRLDEVGIRRQLNIVSKI
Sbjct: 355 YDPEIGYCQGMSDLLSPIITVISEDHEAFWCFVGFMRKARHNFRLDEVGIRRQLNIVSKI 414

Query: 361 IRCKDSHLYKHLEKLQAEDCFFVYRMVVVLFRRELTFEQTLCLWEVIWADQAAIRAGIGK 420
           IRCKDSHLYKHLEKL+AEDCFFVYRMVVVLFRRELTFEQTLCLWEVIWADQAAIRAGIGK
Sbjct: 415 IRCKDSHLYKHLEKLEAEDCFFVYRMVVVLFRRELTFEQTLCLWEVIWADQAAIRAGIGK 474

Query: 421 SAWSRIRQRAPPTDDLLLYAIAASVLQRRKLIIEKYSSMDEILRECNSMAGHLDVWKLLD 480
           SAWSRIRQRAPPTDDLLLYAIAASVLQRRKLIIEKY+SMDEILRECNSMAGHLDVWKLLD
Sbjct: 475 SAWSRIRQRAPPTDDLLLYAIAASVLQRRKLIIEKYNSMDEILRECNSMAGHLDVWKLLD 534

Query: 481 GAHNLVVTLHDKIETSF 497
           GAH+LVVTLHDKIETSF
Sbjct: 535 GAHDLVVTLHDKIETSF 549

BLAST of MS006173 vs. ExPASy TrEMBL
Match: A0A0A0KQ34 (Rab-GAP TBC domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G139150 PE=4 SV=1)

HSP 1 Score: 923.7 bits (2386), Expect = 3.3e-265
Identity = 469/497 (94.37%), Postives = 483/497 (97.18%), Query Frame = 0

Query: 1   RCRLKSPWSRRKRKNALSSRQWRTVFNPDGKLRDGGIKFVKKVRSGGVDPSIRAEVWPFL 60
           R RLKSPWSRRKRK+ALS RQW+TVF+PDGKLRDGGIKF+KKVRSGGVDPSIRAEVWPFL
Sbjct: 63  RSRLKSPWSRRKRKHALSPRQWKTVFSPDGKLRDGGIKFLKKVRSGGVDPSIRAEVWPFL 122

Query: 61  LGVYDLKSSKEERDIIKTQKRKEYEKLRKQCRRLIKRRNESSKWNEFGDMI-VGEDGFRF 120
           LGVYDLKSSK+ERDIIKTQKRKEYEKLRKQCRRLIKRRNESSKWNEF DMI VGEDG  F
Sbjct: 123 LGVYDLKSSKKERDIIKTQKRKEYEKLRKQCRRLIKRRNESSKWNEFRDMIDVGEDG--F 182

Query: 121 LVRDIDSPSSEDVVSARESLSSEERCSNVEFLDEPFCSLLEGEGSSRQIAADGSLVLNSD 180
           LV+DIDSPSSEDVVSARESLSSEERCSNVEFLDE FCSLLEGEGSSR+I AD SLVLNSD
Sbjct: 183 LVQDIDSPSSEDVVSARESLSSEERCSNVEFLDEAFCSLLEGEGSSRRITADSSLVLNSD 242

Query: 181 SSDSDSSDDLDVSQTFPPTDGMEEPYLDMTPKENSSPPRTEVSSKLHNSEDFATWQRIIR 240
           SSDSDSSDDLD+SQTFP TDGMEEPYLDMT KENSSP RTEVSSKLHNSEDFATWQRIIR
Sbjct: 243 SSDSDSSDDLDLSQTFPSTDGMEEPYLDMTAKENSSPTRTEVSSKLHNSEDFATWQRIIR 302

Query: 241 LDALRSNSEWVPYLTSQAMVSDGRARRSAEAVGLVDYDHLEPCMIFHAARLVAILEAYAL 300
           LDALRSNSEWVPYL+SQA VSDGRARR AEAVGLVDYDHLEPCMIFHAARLVAILEAYAL
Sbjct: 303 LDALRSNSEWVPYLSSQAPVSDGRARRCAEAVGLVDYDHLEPCMIFHAARLVAILEAYAL 362

Query: 301 YDPEIGYCQGMSDLLSPIITVIAEDHEAFWCFVGFMRKARHNFRLDEVGIRRQLNIVSKI 360
           +DPEIGYCQGMSDLLSPIITVI+EDHEAFWCFVGFMRKARHNFRLDEVGIRRQLNIVSKI
Sbjct: 363 FDPEIGYCQGMSDLLSPIITVISEDHEAFWCFVGFMRKARHNFRLDEVGIRRQLNIVSKI 422

Query: 361 IRCKDSHLYKHLEKLQAEDCFFVYRMVVVLFRRELTFEQTLCLWEVIWADQAAIRAGIGK 420
           IRCKDSHLYKHLEKL+AEDCFFVYRMVVVLFRRELTFEQTLCLWEVIWADQAAIRAGIGK
Sbjct: 423 IRCKDSHLYKHLEKLEAEDCFFVYRMVVVLFRRELTFEQTLCLWEVIWADQAAIRAGIGK 482

Query: 421 SAWSRIRQRAPPTDDLLLYAIAASVLQRRKLIIEKYSSMDEILRECNSMAGHLDVWKLLD 480
           SAWSRIRQRAPPTDDLLLYAIAASVLQRRKLIIEKY+SMDEILRECNSMAGHLDVWKLLD
Sbjct: 483 SAWSRIRQRAPPTDDLLLYAIAASVLQRRKLIIEKYNSMDEILRECNSMAGHLDVWKLLD 542

Query: 481 GAHNLVVTLHDKIETSF 497
           GAH+LVVTLHDKIETSF
Sbjct: 543 GAHDLVVTLHDKIETSF 557

BLAST of MS006173 vs. ExPASy TrEMBL
Match: A0A6J1K4C8 (GTPase-activating protein gyp7-like OS=Cucurbita maxima OX=3661 GN=LOC111491068 PE=4 SV=1)

HSP 1 Score: 904.8 bits (2337), Expect = 1.6e-259
Identity = 461/498 (92.57%), Postives = 477/498 (95.78%), Query Frame = 0

Query: 1   RCRLKSPWSRRKRKNALSSRQWRTVFNPDGKLRDGGIKFVKKVRSGGVDPSIRAEVWPFL 60
           R RLKSPWSRRKRK+ALS RQW+TVFN DGKLRD GIKF+KKVRSGGVD SIRAEVWPFL
Sbjct: 49  RTRLKSPWSRRKRKHALSPRQWKTVFNLDGKLRDSGIKFLKKVRSGGVDSSIRAEVWPFL 108

Query: 61  LGVYDLKSSKEERDIIKTQKRKEYEKLRKQCRRLIKRRNESSKWNEFGDMI-VGEDGFRF 120
           LGVYDLKSSKEERDIIKTQKRKEYEKLRK+CRRLIKRRNESSKWNEFGDMI VGEDG  F
Sbjct: 109 LGVYDLKSSKEERDIIKTQKRKEYEKLRKRCRRLIKRRNESSKWNEFGDMIDVGEDG--F 168

Query: 121 LVRDIDSPSSEDVVSARESLSSEERCSNVEFLDEPFCSLLEGEGSSRQIAADGSLVLNSD 180
           LVRD+DSPSSEDVVSARESLSSEERCSNV+FLDEPF SLLEGEGSSR+I ADGSLVLNSD
Sbjct: 169 LVRDVDSPSSEDVVSARESLSSEERCSNVDFLDEPFNSLLEGEGSSRRITADGSLVLNSD 228

Query: 181 SSDSDSSDDLDVSQTFPPTDGMEEPYLDMTPKENSSPPRTEV-SSKLHNSEDFATWQRII 240
           SSDS+SSDDLDVSQ FP TDGMEEP+LD  PKENSSP R E+ SSKLH+ EDFATWQRII
Sbjct: 229 SSDSESSDDLDVSQAFPSTDGMEEPFLDSMPKENSSPSRAEISSSKLHSGEDFATWQRII 288

Query: 241 RLDALRSNSEWVPYLTSQAMVSDGRARRSAEAVGLVDYDHLEPCMIFHAARLVAILEAYA 300
           RLDALRSNSEWVPYL+SQAMVSDGRARR AEAVGLVDYDHLEPCMIFHAARLVAILEAYA
Sbjct: 289 RLDALRSNSEWVPYLSSQAMVSDGRARRCAEAVGLVDYDHLEPCMIFHAARLVAILEAYA 348

Query: 301 LYDPEIGYCQGMSDLLSPIITVIAEDHEAFWCFVGFMRKARHNFRLDEVGIRRQLNIVSK 360
           LYDPEIGYCQGMSDLLSPIITVI EDHEAFWCFVGFMRKARHNFRLDEVGIRRQL+IVSK
Sbjct: 349 LYDPEIGYCQGMSDLLSPIITVIPEDHEAFWCFVGFMRKARHNFRLDEVGIRRQLSIVSK 408

Query: 361 IIRCKDSHLYKHLEKLQAEDCFFVYRMVVVLFRRELTFEQTLCLWEVIWADQAAIRAGIG 420
           II+CKD HLYKHLEKLQAEDCFFVYRMVVVLFRRELTFEQTLCLWEVIWADQAAIRAG+G
Sbjct: 409 IIKCKDPHLYKHLEKLQAEDCFFVYRMVVVLFRRELTFEQTLCLWEVIWADQAAIRAGMG 468

Query: 421 KSAWSRIRQRAPPTDDLLLYAIAASVLQRRKLIIEKYSSMDEILRECNSMAGHLDVWKLL 480
           KSAWSRIRQRAPPTDDLLLYAIAASVLQRRKLIIEKY+SMDEILRECNSMAGHLDVWKLL
Sbjct: 469 KSAWSRIRQRAPPTDDLLLYAIAASVLQRRKLIIEKYNSMDEILRECNSMAGHLDVWKLL 528

Query: 481 DGAHNLVVTLHDKIETSF 497
           DGAHNLVVTLHDKIETSF
Sbjct: 529 DGAHNLVVTLHDKIETSF 544

BLAST of MS006173 vs. TAIR 10
Match: AT3G49350.1 (Ypt/Rab-GAP domain of gyp1p superfamily protein )

HSP 1 Score: 630.6 bits (1625), Expect = 1.1e-180
Identity = 333/498 (66.87%), Postives = 394/498 (79.12%), Query Frame = 0

Query: 1   RCRLKSPWSRRKRKNALSSRQWRTVFNPDGKLRDGGIKFVKKVRSGGVDPSIRAEVWPFL 60
           R RLKSPWSRRKRK  L  +QW+  F PDG+LR+GG+  +KKVRS G++PSIR EVWPFL
Sbjct: 53  RPRLKSPWSRRKRKRPLRPQQWKRFFTPDGRLRNGGVDLLKKVRSRGIEPSIRLEVWPFL 112

Query: 61  LGVYDLKSSKEERDIIKTQKRKEYEKLRKQCRRLIKRRNESSKWNEFGDMIVGEDGFRFL 120
           LG+Y   SSKEER  I+ ++RKEYE+LR+QC+RL K  N + K     + I  E  +   
Sbjct: 113 LGLYGFNSSKEERVTIRNRRRKEYERLRRQCKRLQKHNNGTRKLYNGSETIQDEYDWP-R 172

Query: 121 VRDIDSPSSEDVVSARESLSSEERCS-NVEFLDEPFCSLLEGEG-SSRQIAADGSLVLNS 180
           V+D DS  S++VVSARESLSS+E  + ++ ++ E  C++   +G SSR+I       LNS
Sbjct: 173 VQDTDSSCSDEVVSARESLSSDEDITEDIGYMSEVSCTVERDDGSSSRRITNATISTLNS 232

Query: 181 DSSDSDSSDDLDVSQTFPPTDGMEEPYLDMTPKENSSPPRTEVSSKLHNSEDFATWQRII 240
           +SSDSDSSD+ +V Q F        P ++ T   +SS PRTE        EDF TWQRII
Sbjct: 233 ESSDSDSSDESEVVQVF---QSSATPDVNSTYPASSSIPRTE--------EDFVTWQRII 292

Query: 241 RLDALRSNSEWVPYLTSQAMVSDGRARRSAEAVGLVDYDHLEPCMIFHAARLVAILEAYA 300
           RLDA+R++SEW  Y  SQA++S+ RA R+AEAVGL DY+HLEP  IF AARLVA+LEAYA
Sbjct: 293 RLDAVRADSEWTSYSPSQAVISEDRACRAAEAVGLKDYNHLEPYKIFQAARLVAVLEAYA 352

Query: 301 LYDPEIGYCQGMSDLLSPIITVIAEDHEAFWCFVGFMRKARHNFRLDEVGIRRQLNIVSK 360
           LYDP+IGYCQGMSDLLSPI++VI +DHE FWCFVGFM+KARHNFRLDEVGIRRQLNIVSK
Sbjct: 353 LYDPDIGYCQGMSDLLSPILSVIPDDHEVFWCFVGFMKKARHNFRLDEVGIRRQLNIVSK 412

Query: 361 IIRCKDSHLYKHLEKLQAEDCFFVYRMVVVLFRRELTFEQTLCLWEVIWADQAAIRAGIG 420
           II+ KDS LY+HLEKLQAEDCFFVYRMVVV+FRRELT +QTLCLWEV+WADQAAIRAG+G
Sbjct: 413 IIKSKDSQLYRHLEKLQAEDCFFVYRMVVVMFRRELTLDQTLCLWEVMWADQAAIRAGMG 472

Query: 421 KSAWSRIRQRAPPTDDLLLYAIAASVLQRRKLIIEKYSSMDEILRECNSMAGHLDVWKLL 480
           KSAWSRIRQRAPPTDDL+LYAIAASVLQRRK IIE+Y+SMDEILREC SMAG LDVWKLL
Sbjct: 473 KSAWSRIRQRAPPTDDLVLYAIAASVLQRRKRIIERYNSMDEILRECQSMAGQLDVWKLL 532

Query: 481 DGAHNLVVTLHDKIETSF 497
           D AH+LVVTLH KIE SF
Sbjct: 533 DDAHDLVVTLHTKIEHSF 538

BLAST of MS006173 vs. TAIR 10
Match: AT5G24390.1 (Ypt/Rab-GAP domain of gyp1p superfamily protein )

HSP 1 Score: 587.8 bits (1514), Expect = 8.1e-168
Identity = 308/496 (62.10%), Postives = 381/496 (76.81%), Query Frame = 0

Query: 3   RLKSPWSRRKRKNALSSRQWRTVFNPDGKLRDGGIKFVKKVRSGGVDPSIRAEVWPFLLG 62
           RLKSPWSRRK K  L+ R+WR  F P+G++R+GG+  +KKVR+ G+DPSIR+EVWPFLLG
Sbjct: 49  RLKSPWSRRKGKKPLTLRRWRRCFTPEGRIRNGGVDLLKKVRTRGIDPSIRSEVWPFLLG 108

Query: 63  VYDLKSSKEERDIIKTQKRKEYEKLRKQCRRLIKRRNESSKWNEFGDMIVGEDGFRF-LV 122
           V D  SS+EER   +T +RK YE+LR+QC+RL K+ + + K N+       ++   + L 
Sbjct: 109 VCDFNSSEEERGATRTWRRKVYERLRRQCKRLQKQNSGTFKLNKINKTTQDDEHDSWSLA 168

Query: 123 RDIDSPSSEDVVSARESL-SSEERCSNVEFLDEPFCSLLEGEGSSRQIAADGSLVLNSDS 182
           +D +S  S+D  S  ESL S ++   ++ ++ +  C+L      SR         LNS+S
Sbjct: 169 QDSESSCSDDACSVHESLISDKDNTEDIGYMSDVSCTLDRDYNGSRH--------LNSES 228

Query: 183 SDSDSSDDLDVSQTFPPTDGMEEPYLDMTPKENSSPPRTEVSSKLHNSEDFATWQRIIRL 242
           SDSDSSD+ D  Q  P  +G +         ENS+ P +   S+    EDF TWQRIIRL
Sbjct: 229 SDSDSSDENDYVQVAPSCEGKD---------ENSTSPSSIYISR--TKEDFVTWQRIIRL 288

Query: 243 DALRSNSEWVPYLTSQAMVSDGRARRSAEAVGLVDYDHLEPCMIFHAARLVAILEAYALY 302
           DALR+++EW PY  SQA+VS+ RARR+AEAVGL DY +LEP  IFHAARLVA+LEAYAL+
Sbjct: 289 DALRADTEWTPYSQSQALVSENRARRAAEAVGLKDYSYLEPSKIFHAARLVAVLEAYALH 348

Query: 303 DPEIGYCQGMSDLLSPIITVIAEDHEAFWCFVGFMRKARHNFRLDEVGIRRQLNIVSKII 362
           DPEIGYCQGMSDLLSPI++VI +D+EAFWCFVGFM+KAR NFR+DEVGI RQLNIVSKII
Sbjct: 349 DPEIGYCQGMSDLLSPILSVIPDDYEAFWCFVGFMKKARQNFRVDEVGITRQLNIVSKII 408

Query: 363 RCKDSHLYKHLEKLQAEDCFFVYRMVVVLFRRELTFEQTLCLWEVIWADQAAIRAGIGKS 422
           + KDS LYKHLEK++AEDCFFVYRMV+V+FRRELT EQTL LWEVIWADQAAIRAG+GKS
Sbjct: 409 KSKDSQLYKHLEKVKAEDCFFVYRMVLVMFRRELTLEQTLHLWEVIWADQAAIRAGMGKS 468

Query: 423 AWS-RIRQRAPPTDDLLLYAIAASVLQRRKLIIEKYSSMDEILRECNSMAGHLDVWKLLD 482
           +WS RI+QRAPPT+DLLLY +AASVLQRRK+IIEKYSSM+EILREC++M G LDVWKLLD
Sbjct: 469 SWSRRIKQRAPPTEDLLLYVVAASVLQRRKVIIEKYSSMEEILRECHNMVGKLDVWKLLD 525

Query: 483 GAHNLVVTLHDKIETS 496
            AH+L+VTLH KIE S
Sbjct: 529 DAHDLIVTLHAKIEHS 525

BLAST of MS006173 vs. TAIR 10
Match: AT5G53570.1 (Ypt/Rab-GAP domain of gyp1p superfamily protein )

HSP 1 Score: 574.3 bits (1479), Expect = 9.3e-164
Identity = 311/500 (62.20%), Postives = 374/500 (74.80%), Query Frame = 0

Query: 3   RLKSPWSRRKRKNALSSRQWRTVFNPDGKLRDGGIKFVKKVRSGGVDPSIRAEVWPFLLG 62
           R KSPWSRRKRK AL+  QWR++F P+GKLRDGG+ F+KKVRS GVDPSIRAEVW FLLG
Sbjct: 82  RRKSPWSRRKRKWALTPHQWRSLFTPEGKLRDGGVGFLKKVRSRGVDPSIRAEVWLFLLG 141

Query: 63  VYDLKSSKEERDIIKTQKRKEYEKLRKQCRRLIKRRNESSKWNEFGDMIVGEDGFRFLVR 122
           VYDL S+ EER+ +KTQKRKEYEKL+++C+ L+K  N S+                    
Sbjct: 142 VYDLNSTSEEREAVKTQKRKEYEKLQRRCQMLLKCGNGSTD------------------- 201

Query: 123 DIDSPSSEDVVSARESLSSEERCSNVEFLDEPFCSLLEGEGSSRQIAADGSLVLNSDSSD 182
                      +  E  S E     V F+D+     + G  +S+ + +     LN+DSSD
Sbjct: 202 -----------NLEELPSDEANSQCVRFVDD---YKITGPMTSQDVVS----ALNTDSSD 261

Query: 183 SDS---SDDLDVSQTFPPTDGM--EEPYLDMTPKENSS---PPRTEVSSKLHNSEDFATW 242
           +DS   ++D+ +  +F  +D    EE   +   +ENSS      +EV  ++   EDF+TW
Sbjct: 262 TDSCEDNEDVLLLSSFAHSDEKKPEEDNSNNNSEENSSLLVAAASEVQVEVAVHEDFSTW 321

Query: 243 QRIIRLDALRSNSEWVPYLTSQAMVSDGRARRSAEAVGLVDYDHLEPCMIFHAARLVAIL 302
           QRIIRLDALR++SEW  Y      +++ +ARR AE+VGL DYDHLE C ++HAARLVAIL
Sbjct: 322 QRIIRLDALRADSEWANYSPYSTAITESKARRLAESVGLKDYDHLESCRLYHAARLVAIL 381

Query: 303 EAYALYDPEIGYCQGMSDLLSPIITVIAEDHEAFWCFVGFMRKARHNFRLDEVGIRRQLN 362
           EAYA+YDPEIGYCQGMSDLLSPI+ VI+EDHEAFWCFVGFM+KARHNFRLDE GI+RQL+
Sbjct: 382 EAYAMYDPEIGYCQGMSDLLSPILAVISEDHEAFWCFVGFMKKARHNFRLDEAGIQRQLS 441

Query: 363 IVSKIIRCKDSHLYKHLEKLQAEDCFFVYRMVVVLFRRELTFEQTLCLWEVIWADQAAIR 422
           IVSKII+ KDS LYKHLE LQAEDC FVYRMV+V+FRREL+FEQTLCLWEV+WADQAAIR
Sbjct: 442 IVSKIIKNKDSQLYKHLENLQAEDCSFVYRMVLVMFRRELSFEQTLCLWEVMWADQAAIR 501

Query: 423 AGIGKSAWSRIRQRAPPTDDLLLYAIAASVLQRRKLIIEKYSSMDEILRECNSMAGHLDV 482
           AG+GKS WSRIRQ+APPTDDLLLYAIAA VL RRKLII+KYSSMDEI+ ECNSMAG L+V
Sbjct: 502 AGVGKSPWSRIRQQAPPTDDLLLYAIAALVL-RRKLIIQKYSSMDEIVEECNSMAGQLNV 543

Query: 483 WKLLDGAHNLVVTLHDKIET 495
           WKLLD AH+LVVTLHDKIET
Sbjct: 562 WKLLDDAHHLVVTLHDKIET 543

BLAST of MS006173 vs. TAIR 10
Match: AT5G53570.2 (Ypt/Rab-GAP domain of gyp1p superfamily protein )

HSP 1 Score: 574.3 bits (1479), Expect = 9.3e-164
Identity = 311/500 (62.20%), Postives = 374/500 (74.80%), Query Frame = 0

Query: 3   RLKSPWSRRKRKNALSSRQWRTVFNPDGKLRDGGIKFVKKVRSGGVDPSIRAEVWPFLLG 62
           R KSPWSRRKRK AL+  QWR++F P+GKLRDGG+ F+KKVRS GVDPSIRAEVW FLLG
Sbjct: 109 RRKSPWSRRKRKWALTPHQWRSLFTPEGKLRDGGVGFLKKVRSRGVDPSIRAEVWLFLLG 168

Query: 63  VYDLKSSKEERDIIKTQKRKEYEKLRKQCRRLIKRRNESSKWNEFGDMIVGEDGFRFLVR 122
           VYDL S+ EER+ +KTQKRKEYEKL+++C+ L+K  N S+                    
Sbjct: 169 VYDLNSTSEEREAVKTQKRKEYEKLQRRCQMLLKCGNGSTD------------------- 228

Query: 123 DIDSPSSEDVVSARESLSSEERCSNVEFLDEPFCSLLEGEGSSRQIAADGSLVLNSDSSD 182
                      +  E  S E     V F+D+     + G  +S+ + +     LN+DSSD
Sbjct: 229 -----------NLEELPSDEANSQCVRFVDD---YKITGPMTSQDVVS----ALNTDSSD 288

Query: 183 SDS---SDDLDVSQTFPPTDGM--EEPYLDMTPKENSS---PPRTEVSSKLHNSEDFATW 242
           +DS   ++D+ +  +F  +D    EE   +   +ENSS      +EV  ++   EDF+TW
Sbjct: 289 TDSCEDNEDVLLLSSFAHSDEKKPEEDNSNNNSEENSSLLVAAASEVQVEVAVHEDFSTW 348

Query: 243 QRIIRLDALRSNSEWVPYLTSQAMVSDGRARRSAEAVGLVDYDHLEPCMIFHAARLVAIL 302
           QRIIRLDALR++SEW  Y      +++ +ARR AE+VGL DYDHLE C ++HAARLVAIL
Sbjct: 349 QRIIRLDALRADSEWANYSPYSTAITESKARRLAESVGLKDYDHLESCRLYHAARLVAIL 408

Query: 303 EAYALYDPEIGYCQGMSDLLSPIITVIAEDHEAFWCFVGFMRKARHNFRLDEVGIRRQLN 362
           EAYA+YDPEIGYCQGMSDLLSPI+ VI+EDHEAFWCFVGFM+KARHNFRLDE GI+RQL+
Sbjct: 409 EAYAMYDPEIGYCQGMSDLLSPILAVISEDHEAFWCFVGFMKKARHNFRLDEAGIQRQLS 468

Query: 363 IVSKIIRCKDSHLYKHLEKLQAEDCFFVYRMVVVLFRRELTFEQTLCLWEVIWADQAAIR 422
           IVSKII+ KDS LYKHLE LQAEDC FVYRMV+V+FRREL+FEQTLCLWEV+WADQAAIR
Sbjct: 469 IVSKIIKNKDSQLYKHLENLQAEDCSFVYRMVLVMFRRELSFEQTLCLWEVMWADQAAIR 528

Query: 423 AGIGKSAWSRIRQRAPPTDDLLLYAIAASVLQRRKLIIEKYSSMDEILRECNSMAGHLDV 482
           AG+GKS WSRIRQ+APPTDDLLLYAIAA VL RRKLII+KYSSMDEI+ ECNSMAG L+V
Sbjct: 529 AGVGKSPWSRIRQQAPPTDDLLLYAIAALVL-RRKLIIQKYSSMDEIVEECNSMAGQLNV 570

Query: 483 WKLLDGAHNLVVTLHDKIET 495
           WKLLD AH+LVVTLHDKIET
Sbjct: 589 WKLLDDAHHLVVTLHDKIET 570

BLAST of MS006173 vs. TAIR 10
Match: AT5G41940.1 (Ypt/Rab-GAP domain of gyp1p superfamily protein )

HSP 1 Score: 549.7 bits (1415), Expect = 2.5e-156
Identity = 295/494 (59.72%), Postives = 355/494 (71.86%), Query Frame = 0

Query: 1   RCRLKSPWSRRKRKNALSSRQWRTVFNPDGKLRDGGIKFVKKVRSGGVDPSIRAEVWPFL 60
           R  + SPWS R+RK  L  +QW   F  +G+L DGG+KF+KKVRSGGV PSIR EVWPFL
Sbjct: 73  RGSIGSPWSLRRRKRVLQPKQWNAFFTEEGRLSDGGVKFLKKVRSGGVHPSIRPEVWPFL 132

Query: 61  LGVYDLKSSKEERDIIKTQKRKEYEKLRKQCRRLIKRRNESSKWNEFGDMIVGEDGFRFL 120
           LGVYDLKS+KEERD I+  K  EYE LR+QCR + +R        E       ED     
Sbjct: 133 LGVYDLKSNKEERDSIRQLKLTEYENLRRQCREIHERYENVCDSKETAQSSNTEDS---- 192

Query: 121 VRDIDSPSSEDVVSARESLSSEERCSNVEFLDEPFCSLLEGEGSSRQIAADGSLVLNSDS 180
            + ++S   E+V S+  +++ +E               L  E   +    + S V   D+
Sbjct: 193 -QVLESHDIEEVESSTRTITVDE------------SEKLNSESIMQDENCEKSDVTTEDA 252

Query: 181 SDSDS-SDDLDVSQTFPPTDGME-EPYLDMTPKENSSPPRTEVSSKLHNSEDFATWQRII 240
           + +DS S + + ++T P     E E +  +  ++  S P ++  S+  + +  +TWQRII
Sbjct: 253 AGNDSDSTNPEETETSPFLANEEVESHNTVNQEKEISSPSSKPKSQAADEDFMSTWQRII 312

Query: 241 RLDALRSNSEWVPYLTSQAMVSDGRARRSAEAVGLVDYDHLEPCMIFHAARLVAILEAYA 300
           RLDA+R+N EWVPY  +QA VS+ +AR  A  VGL DYDHLEPC IFHAARLV ILEAYA
Sbjct: 313 RLDAVRANDEWVPYSPTQAAVSETKARGIAIQVGLNDYDHLEPCRIFHAARLVGILEAYA 372

Query: 301 LYDPEIGYCQGMSDLLSPIITVIAEDHEAFWCFVGFMRKARHNFRLDEVGIRRQLNIVSK 360
           +YDPEIGYCQGMSDLLSP+I V+ +D  AFWCFVGFM KARHNFRLDEVGIRRQL++VSK
Sbjct: 373 VYDPEIGYCQGMSDLLSPLIAVMEDDVLAFWCFVGFMSKARHNFRLDEVGIRRQLSMVSK 432

Query: 361 IIRCKDSHLYKHLEKLQAEDCFFVYRMVVVLFRRELTFEQTLCLWEVIWADQAAIRAGIG 420
           II+ KD HLY+HLE L+AEDCFFVYRMVVVLFRRELTFEQTLCLWEV+WADQAAIR GI 
Sbjct: 433 IIKFKDIHLYRHLENLEAEDCFFVYRMVVVLFRRELTFEQTLCLWEVMWADQAAIRTGIA 492

Query: 421 KSAWSRIRQRAPPTDDLLLYAIAASVLQRRKLIIEKYSSMDEILRECNSMAGHLDVWKLL 480
           K+ W RIR RAPPT+DLLLYAIAASVLQRRK IIEKYS MDEI++ECNSMAGHLDVWKLL
Sbjct: 493 KATWGRIRLRAPPTEDLLLYAIAASVLQRRKTIIEKYSGMDEIMKECNSMAGHLDVWKLL 549

Query: 481 DGAHNLVVTLHDKI 493
           D AH+LVV LHDKI
Sbjct: 553 DDAHDLVVNLHDKI 549

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022137806.15.1e-28199.40GTPase-activating protein gyp7-like [Momordica charantia][more]
XP_008437644.11.4e-26594.57PREDICTED: GTPase-activating protein gyp7-like [Cucumis melo] >KAA0042529.1 GTPa... [more]
XP_004145946.26.7e-26594.37GTPase-activating protein gyp7 [Cucumis sativus] >KGN49836.1 hypothetical protei... [more]
XP_038875646.15.3e-26293.76GTPase-activating protein gyp7-like [Benincasa hispida][more]
XP_022995580.13.2e-25992.57GTPase-activating protein gyp7-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q94BY91.3e-16262.20Rab GTPase-activating protein 22 OS=Arabidopsis thaliana OX=3702 GN=RABGAP22 PE=... [more]
P093792.3e-2631.73GTPase-activating protein GYP7 OS=Yarrowia lipolytica (strain CLIB 122 / E 150) ... [more]
Q9UUH71.1e-2323.29GTPase-activating protein gyp7 OS=Schizosaccharomyces pombe (strain 972 / ATCC 2... [more]
Q8TC077.0e-2331.72TBC1 domain family member 15 OS=Homo sapiens OX=9606 GN=TBC1D15 PE=1 SV=2[more]
Q6BU762.0e-2229.17GTPase-activating protein GYP7 OS=Debaryomyces hansenii (strain ATCC 36239 / CBS... [more]
Match NameE-valueIdentityDescription
A0A6J1C7S12.5e-28199.40GTPase-activating protein gyp7-like OS=Momordica charantia OX=3673 GN=LOC1110091... [more]
A0A5A7TMD76.6e-26694.57GTPase-activating protein gyp7-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5... [more]
A0A1S3AUI76.6e-26694.57GTPase-activating protein gyp7-like OS=Cucumis melo OX=3656 GN=LOC103482988 PE=4... [more]
A0A0A0KQ343.3e-26594.37Rab-GAP TBC domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G139150... [more]
A0A6J1K4C81.6e-25992.57GTPase-activating protein gyp7-like OS=Cucurbita maxima OX=3661 GN=LOC111491068 ... [more]
Match NameE-valueIdentityDescription
AT3G49350.11.1e-18066.87Ypt/Rab-GAP domain of gyp1p superfamily protein [more]
AT5G24390.18.1e-16862.10Ypt/Rab-GAP domain of gyp1p superfamily protein [more]
AT5G53570.19.3e-16462.20Ypt/Rab-GAP domain of gyp1p superfamily protein [more]
AT5G53570.29.3e-16462.20Ypt/Rab-GAP domain of gyp1p superfamily protein [more]
AT5G41940.12.5e-15659.72Ypt/Rab-GAP domain of gyp1p superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 77..97
NoneNo IPR availableGENE3D1.10.472.80coord: 346..492
e-value: 4.9E-23
score: 83.4
NoneNo IPR availableGENE3D1.10.8.270putative rabgap domain of human tbc1 domain family member 14 like domainscoord: 210..342
e-value: 3.0E-18
score: 67.8
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 175..225
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 175..193
NoneNo IPR availablePANTHERPTHR22957:SF580SMALL G PROTEIN SIGNALING MODULATOR 2-LIKE ISOFORM X1coord: 3..492
NoneNo IPR availablePANTHERPTHR22957TBC1 DOMAIN FAMILY MEMBER GTPASE-ACTIVATING PROTEINcoord: 3..492
IPR000195Rab-GTPase-TBC domainSMARTSM00164tbc_4coord: 44..436
e-value: 2.4E-28
score: 110.2
IPR000195Rab-GTPase-TBC domainPFAMPF00566RabGAP-TBCcoord: 289..410
e-value: 7.1E-32
score: 110.8
IPR000195Rab-GTPase-TBC domainPROSITEPS50086TBC_RABGAPcoord: 47..410
score: 31.664326
IPR035969Rab-GTPase-TBC domain superfamilySUPERFAMILY47923Ypt/Rab-GAP domain of gyp1pcoord: 33..370
IPR035969Rab-GTPase-TBC domain superfamilySUPERFAMILY47923Ypt/Rab-GAP domain of gyp1pcoord: 347..485

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS006173.1MS006173.1mRNA