MS006102 (gene) Bitter gourd (TR) v1

BLAST of MS006102 vs. NCBI nr
Match: ALJ53304.1 (polyphenol oxidase [Luffa aegyptiaca] >APO14263.1 polyphenol oxidase 4 [Luffa aegyptiaca])

HSP 1 Score: 673.3 bits (1736), Expect = 1.9e-189
Identity = 368/592 (62.16%), Postives = 426/592 (71.96%), Query Frame = 0

Query: 1   MASLSSLLSLPPYYHSKTQQSRPQNRRHGVSYHFVPCRASYNNGDETPIIKSNPDEVGIS 60
           MASLSSLLSL P   SK Q S   NRR G+SY  +PCRAS  NG +TP + SNP E G S
Sbjct: 1   MASLSSLLSLFPCIRSKNQSSSRGNRRLGLSY--IPCRAS--NGVQTP-MNSNPQE-GDS 60

Query: 61  VNKFDRRDVLFGLGGLYGVASLSGGDRFALADDTHTISPDTANCGPARTPAGPR-NCCPP 120
           ++KFDRRDVLFGLGGLY  A+L G DR ALAD    ISPD  +C P    A  + +CCPP
Sbjct: 61  MSKFDRRDVLFGLGGLYSAAAL-GRDRSALAD---PISPDVIHCEPITLEADEKVDCCPP 120

Query: 121 KIHDIINFIPPPMIPHPRVRPAAHLITPEQLAKFEKAIYLMKHLPYDDPRRFSNQAQVHC 180
            I DI +F+ PP + + RVRPAAHLIT EQLAK E+A+  MK L  DDPR F  QA+VHC
Sbjct: 121 SILDITDFV-PPAVENLRVRPAAHLITQEQLAKLEEAVTKMKALDPDDPRNFYQQARVHC 180

Query: 181 AYCNYAYKQLDSVPKAELEVHWNALFFPFHRCYLYFFERILGYLIDDPTFAIPFWNWDSP 240
           AYCN AYKQ D  P+ + ++HWN LFFPFHRCYLYF ERILG LI+DP FAIPFWNWD+ 
Sbjct: 181 AYCNDAYKQ-DGFPELDFDIHWNWLFFPFHRCYLYFHERILGSLINDPDFAIPFWNWDAI 240

Query: 241 PGMKMPKIYNNPD--SSLYDRLRDARHVPPKLAYLNYDG-DEDPPFDVVDCNLRWMNKQM 300
           PGM+MP IY N D  S LYD LRD  H PPKL  LNYDG  EDP +DV+ CN R M +QM
Sbjct: 241 PGMQMPAIYINDDKNSPLYDELRDPNHKPPKLLDLNYDGATEDPSYDVLFCNPRLMYRQM 300

Query: 301 KVAGKSARGFLGQLIRRGEEAKTAGMGSIESSPHNNLHDWVGDPNSPHNEDMGTFYSAGN 360
            +  K+A GF G+ +R G+EA   GMGSIE S HNN+H W GDP   + EDMG FY+A  
Sbjct: 301 VINSKTAEGFHGKPLRAGQEAYRTGMGSIELSAHNNVHTWSGDPQQKYREDMGHFYAAAR 360

Query: 361 DPLFYGHHANVDRMWSIWQKL-ETKRRDYTDPDWLDSSFVLYDENKNAVRIKVRDCVDSV 420
           DPLFY HHANVDRMW+IWQ L + K RD+TDPDWL++SFV YDENKNA+RIKV+DC+D+ 
Sbjct: 361 DPLFYAHHANVDRMWTIWQGLIDGKSRDFTDPDWLNASFVFYDENKNAIRIKVQDCLDNE 420

Query: 421 NLGYVYQDVDIQWLNTRQIPRQNPPTSSSPEPPLAPPPEVYFPAMLNSEINTVVGRPSRS 480
            LGY YQ V+I WL+T+ IPRQ      S +    PP EV     LNS++   V RP RS
Sbjct: 421 KLGYKYQAVEIPWLHTKPIPRQKTSQLKSSKAQETPPIEV-----LNSKMTKEVKRPQRS 480

Query: 481 RTIEQKGEEDEVLVIEDIEFDRSSRVHFNVLVNVLDEGLDVSPANIEFAGSFRNIPHAHN 540
           R+  QK  EDEVLVIE +E D +SRVHFNVLVNVLD+G DVSP N EFAGSF++IP  H 
Sbjct: 481 RSKTQKSVEDEVLVIE-MELDTTSRVHFNVLVNVLDDGTDVSPGNTEFAGSFKSIPLGHK 540

Query: 541 HNNAKNSLRFVITELIEELGLDDAETLHVKIAPKLGGENVTIGGIKIELQPI 588
           H  A  SL  VITELIEELG DD ET+ V I PKLGG+ VTIGGIKIELQPI
Sbjct: 541 H-RANTSLSLVITELIEELGYDDDETVFVTIDPKLGGDLVTIGGIKIELQPI 573

BLAST of MS006102 vs. NCBI nr
Match: XP_022994369.1 (polyphenol oxidase, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 660.6 bits (1703), Expect = 1.3e-185
Identity = 338/590 (57.29%), Postives = 423/590 (71.69%), Query Frame = 0

Query: 1   MASLSSLLSLPPYYHSKTQQSRPQNRRHGVSYHFVPCRASYNNGDETPIIKSNPDEVGIS 60
           MAS+S LLS+ PY   K Q S  QN+  G+++  VPCRAS  NGD T             
Sbjct: 1   MASISPLLSILPYSQIKNQTSNRQNKSRGINH--VPCRAS--NGDST------------- 60

Query: 61  VNKFDRRDVLFGLGGLYGVASLSGGDRFALADDTHTISPDTANCGPARTPAGPRN--CCP 120
            +KFDRRDVLFGLGGLYG A+L+     A A     ++PD  NC PA T  G  +  CCP
Sbjct: 61  -SKFDRRDVLFGLGGLYGAAALTNQQSPASA---KPLAPDVNNCEPA-TIVGNEHIPCCP 120

Query: 121 PKIHDIINFIPPPMIPHPRVRPAAHLITPEQLAKFEKAIYLMKHLPYDDPRRFSNQAQVH 180
           PKIHDII+FI PP     R+R  AH +TP+Q   FEKAI++MK L  +DPR F  QA VH
Sbjct: 121 PKIHDIIDFIHPPHTTKLRIRQPAHSLTPKQQFNFEKAIHIMKRLSPNDPRNFYRQADVH 180

Query: 181 CAYCNYAYKQLDSVPKAELEVHWNALFFPFHRCYLYFFERILGYLIDDPTFAIPFWNWDS 240
           CAYC+YAYKQL   P AE++VHWNALFFPFHR YLYFFERILG+LI++P FAIPFWNWD+
Sbjct: 181 CAYCDYAYKQL-GFPNAEMQVHWNALFFPFHRAYLYFFERILGHLINEPDFAIPFWNWDN 240

Query: 241 PPGMKMPKIYNNPDSSLYDRLRDARHVPPKLAYLNYDGDEDPPFDVVDCNLRWMNKQMKV 300
             GM+MP+IY++P+S LYD+LR+ RH P KL  LNY GD++PP+DVVDCNLRWM+KQM  
Sbjct: 241 KKGMQMPEIYDHPNSPLYDKLRNQRHRPHKLLNLNYHGDDNPPYDVVDCNLRWMHKQMIT 300

Query: 301 AGKSARGFLGQLIRRGEEA-KTAGMGSIESSPHNNLHDWVGDPNSPHNEDMGTFYSAGND 360
              +A GF G+L+   +EA +  GMG+IE SPHNN+H+WVGDPN+ +N +MG FYSA  D
Sbjct: 301 DADTAEGFHGKLLVASQEANEETGMGTIERSPHNNIHNWVGDPNAKNNMNMGVFYSAARD 360

Query: 361 PLFYGHHANVDRMWSIWQKLET-KRRDYTDPDWLDSSFVLYDENKNAVRIKVRDCVDSVN 420
           PLFYGHHANVDRMWSIWQ L+  K RD+ +PDWL+SSFV Y+E+K AVRIKV DC+D+  
Sbjct: 361 PLFYGHHANVDRMWSIWQGLKNGKPRDFNNPDWLNSSFVFYNEHKQAVRIKVSDCLDTRR 420

Query: 421 LGYVYQDVDIQWLNTRQIPRQNPPTSSSPEPPLAPPPEVYFPAMLNSEINTVVGRPSRSR 480
           LGYVYQ VD+ WL+T+Q PR      ++  P +     + FP  LNS + T V RP + R
Sbjct: 421 LGYVYQYVDLPWLHTKQTPRLKKQLKTTQAPQV-----IKFPQGLNSMVTTTVKRPKKLR 480

Query: 481 TIEQKGEEDEVLVIEDIEFDRSSRVHFNVLVNVLDEGLDVSPANIEFAGSFRNIPHAHNH 540
             ++K E+DEVLVIE IE D+   VHFNVLVNV+D+G DV P N EF G+F  + H HNH
Sbjct: 481 DEKEKSEKDEVLVIEGIELDKRYWVHFNVLVNVVDDGSDVCPGNTEFVGTFTTVLHGHNH 540

Query: 541 NNAKNSLRFVITELIEELGLDDAETLHVKIAPKLGGENVTIGGIKIELQP 587
              K SLR VITE+++ELG++D E++ V++ PK+GG+ VTIGG+KIELQP
Sbjct: 541 -RGKTSLRVVITEVLKELGIEDDESVIVRLDPKVGGDKVTIGGMKIELQP 561

BLAST of MS006102 vs. NCBI nr
Match: KAG6573251.1 (hypothetical protein SDJN03_27138, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 659.8 bits (1701), Expect = 2.1e-185
Identity = 335/589 (56.88%), Postives = 421/589 (71.48%), Query Frame = 0

Query: 1   MASLSSLLSLPPYYHSKTQQSRPQNRRHGVSYHFVPCRASYNNGDETPIIKSNPDEVGIS 60
           MAS+S LLS+ PY   K Q S  QN+  G+++  VPCRAS  NGD T             
Sbjct: 1   MASISPLLSILPYSQIKNQTSNLQNKSRGINH--VPCRAS--NGDST------------- 60

Query: 61  VNKFDRRDVLFGLGGLYGVASLSGGDRFALADDTHTISPDTANCGPARTPAGPR-NCCPP 120
            +KFDRRDVLFGLGGLYG A+L+     A A     ++PD  NC PA         CCPP
Sbjct: 61  -SKFDRRDVLFGLGGLYGAAALTNQQSPASA---APLAPDVNNCEPATIEGNQHIPCCPP 120

Query: 121 KIHDIINFIPPPMIPHPRVRPAAHLITPEQLAKFEKAIYLMKHLPYDDPRRFSNQAQVHC 180
           KIH II+FI PP     R+RP AH +TP+Q   FEKAI++MK LP +DPR F  QA VHC
Sbjct: 121 KIHHIIDFIHPPHTTKLRIRPPAHSLTPKQQFNFEKAIHIMKRLPPNDPRNFYRQADVHC 180

Query: 181 AYCNYAYKQLDSVPKAELEVHWNALFFPFHRCYLYFFERILGYLIDDPTFAIPFWNWDSP 240
           AYC+YAYKQL   P AE++VHWNALFFPFHR YLYFFERILG+LI++P F IPFWNWD+ 
Sbjct: 181 AYCDYAYKQL-GFPNAEMQVHWNALFFPFHRAYLYFFERILGHLINEPNFVIPFWNWDNT 240

Query: 241 PGMKMPKIYNNPDSSLYDRLRDARHVPPKLAYLNYDGDEDPPFDVVDCNLRWMNKQMKVA 300
            GM+MP+IY++P+S LYD+LR+ RH P KL  LNY GD++P +DVVD NLRWM+KQM   
Sbjct: 241 EGMQMPEIYDHPNSPLYDKLRNQRHRPHKLLNLNYHGDDNPSYDVVDSNLRWMHKQMIKD 300

Query: 301 GKSARGFLGQLIRRGEEA-KTAGMGSIESSPHNNLHDWVGDPNSPHNEDMGTFYSAGNDP 360
             +A GFLG+L+   +EA +  GMG+IE SPHNN+H+WVGDPN+ ++ +MG FYSA  DP
Sbjct: 301 ADTAEGFLGKLLVASDEANEETGMGTIERSPHNNIHNWVGDPNAKNHMNMGAFYSAARDP 360

Query: 361 LFYGHHANVDRMWSIWQKLET-KRRDYTDPDWLDSSFVLYDENKNAVRIKVRDCVDSVNL 420
           LFYGHHANVDRMWSIWQ L+  K RD+ +PDWL+SSFV Y+E+K AVRIKVRDC+D+  L
Sbjct: 361 LFYGHHANVDRMWSIWQGLKNGKPRDFDNPDWLNSSFVFYNEHKQAVRIKVRDCLDTRRL 420

Query: 421 GYVYQDVDIQWLNTRQIPRQNPPTSSSPEPPLAPPPEVYFPAMLNSEINTVVGRPSRSRT 480
           GYVYQ VD+ WL+T+Q PR      ++  P +     + FP  LNS + T V RP++   
Sbjct: 421 GYVYQYVDLPWLHTKQTPRLKKQLKTTQAPQV-----IKFPQGLNSMVTTTVKRPNKLMN 480

Query: 481 IEQKGEEDEVLVIEDIEFDRSSRVHFNVLVNVLDEGLDVSPANIEFAGSFRNIPHAHNHN 540
            ++K E+DEVLVIE IE D+   VHFNVLVNV+D+G DV P N EF G+F  +PH HNH 
Sbjct: 481 EKEKSEKDEVLVIEGIELDKRFWVHFNVLVNVVDDGSDVCPGNTEFVGTFTTVPHGHNH- 540

Query: 541 NAKNSLRFVITELIEELGLDDAETLHVKIAPKLGGENVTIGGIKIELQP 587
             K SLR VITE+++ELG++D E++ V++ PK+GG+ VTIGG+KIELQP
Sbjct: 541 RGKTSLRVVITEVLKELGIEDDESVIVRLDPKVGGDKVTIGGMKIELQP 561

BLAST of MS006102 vs. NCBI nr
Match: XP_022954613.1 (polyphenol oxidase, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 656.8 bits (1693), Expect = 1.8e-184
Identity = 334/589 (56.71%), Postives = 418/589 (70.97%), Query Frame = 0

Query: 1   MASLSSLLSLPPYYHSKTQQSRPQNRRHGVSYHFVPCRASYNNGDETPIIKSNPDEVGIS 60
           MAS+S LLS+ PY   K Q S  QN+  G++   VPCRAS  NGD T             
Sbjct: 1   MASISPLLSILPYSQIKNQTSNLQNKSRGINQ--VPCRAS--NGDST------------- 60

Query: 61  VNKFDRRDVLFGLGGLYGVASLSGGDRFALADDTHTISPDTANCGPARTPAGPR-NCCPP 120
            +KFDRRDVLFGLGGLYG A+L+     A A     ++PD  NC  A         CCPP
Sbjct: 61  -SKFDRRDVLFGLGGLYGAAALTNQQSPASA---APLAPDVNNCEAATIEGNEHIPCCPP 120

Query: 121 KIHDIINFIPPPMIPHPRVRPAAHLITPEQLAKFEKAIYLMKHLPYDDPRRFSNQAQVHC 180
           KIHDII+FI PP     R+RP AH +TP+Q   FEKAI++MK LP +DPR F  QA VHC
Sbjct: 121 KIHDIIDFIHPPHTTKLRIRPPAHSLTPKQQFNFEKAIHIMKRLPPNDPRNFYRQADVHC 180

Query: 181 AYCNYAYKQLDSVPKAELEVHWNALFFPFHRCYLYFFERILGYLIDDPTFAIPFWNWDSP 240
           AYC+YAYKQL   P AE++VHWNALFFPFHR YLYFFERILG+LI++P F IPFWNWD+ 
Sbjct: 181 AYCDYAYKQL-GFPNAEMQVHWNALFFPFHRAYLYFFERILGHLINEPNFVIPFWNWDNT 240

Query: 241 PGMKMPKIYNNPDSSLYDRLRDARHVPPKLAYLNYDGDEDPPFDVVDCNLRWMNKQMKVA 300
            GM+MP+IY++P+S LYD+LR+ RH P KL  LNY GD++P +DVVD NLRWM+KQM   
Sbjct: 241 EGMQMPEIYDHPNSPLYDKLRNQRHRPHKLLNLNYHGDDNPSYDVVDSNLRWMHKQMITD 300

Query: 301 GKSARGFLGQLIRRGEEA-KTAGMGSIESSPHNNLHDWVGDPNSPHNEDMGTFYSAGNDP 360
             +A GF G+L+    EA +  GMG+IE SPHNN+H+WVGDPN+ ++ +MG FYSA  DP
Sbjct: 301 ANTAEGFHGKLLIASHEANEETGMGTIEKSPHNNIHNWVGDPNAKNHMNMGAFYSAARDP 360

Query: 361 LFYGHHANVDRMWSIWQKLET-KRRDYTDPDWLDSSFVLYDENKNAVRIKVRDCVDSVNL 420
           LFYGHHANVDRMWSIWQ L+  K RD+ +PDWL+SSFV Y+E+K AVRIKVRDC+D+  L
Sbjct: 361 LFYGHHANVDRMWSIWQGLKNGKARDFDNPDWLNSSFVFYNEHKQAVRIKVRDCLDTRRL 420

Query: 421 GYVYQDVDIQWLNTRQIPRQNPPTSSSPEPPLAPPPEVYFPAMLNSEINTVVGRPSRSRT 480
           GYVYQ VD+ WL+T+Q PR      ++  P +     + FP  LNS + T V RP++   
Sbjct: 421 GYVYQYVDLPWLHTKQTPRLKKQLKTTQAPQV-----IKFPQGLNSMVTTTVKRPNKLMN 480

Query: 481 IEQKGEEDEVLVIEDIEFDRSSRVHFNVLVNVLDEGLDVSPANIEFAGSFRNIPHAHNHN 540
            ++K E+DEVLVIE IE D+   VHFNVLVNV+D+G DV P N EF G+F  +PH HNH 
Sbjct: 481 EKEKSEKDEVLVIEGIELDKRFWVHFNVLVNVVDDGSDVCPGNTEFVGTFTTVPHGHNH- 540

Query: 541 NAKNSLRFVITELIEELGLDDAETLHVKIAPKLGGENVTIGGIKIELQP 587
             K SLR VITE+++ELG++D E++ V++ PK+GG+ VTIGG+KIELQP
Sbjct: 541 RGKTSLRVVITEVLKELGIEDDESVIVRLDPKVGGDKVTIGGMKIELQP 561

BLAST of MS006102 vs. NCBI nr
Match: KAG7012418.1 (hypothetical protein SDJN02_25170, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 655.2 bits (1689), Expect = 5.3e-184
Identity = 333/589 (56.54%), Postives = 418/589 (70.97%), Query Frame = 0

Query: 1   MASLSSLLSLPPYYHSKTQQSRPQNRRHGVSYHFVPCRASYNNGDETPIIKSNPDEVGIS 60
           MAS+S LLS+ PY   K Q S  QN+  G+++  VPCRAS  NGD T             
Sbjct: 6   MASISPLLSILPYSQIKNQTSNLQNKSRGINH--VPCRAS--NGDST------------- 65

Query: 61  VNKFDRRDVLFGLGGLYGVASLSGGDRFALADDTHTISPDTANCGPARTPAGPR-NCCPP 120
            +KFDRRDVLFGLGGLYG A+L+     A A     ++PD  NC PA         CCPP
Sbjct: 66  -SKFDRRDVLFGLGGLYGAAALTNQQSPASA---APLAPDVNNCEPATIEGNQHIPCCPP 125

Query: 121 KIHDIINFIPPPMIPHPRVRPAAHLITPEQLAKFEKAIYLMKHLPYDDPRRFSNQAQVHC 180
           KIHDII+FI PP     R+RP AH +TP+Q   FEKAI++MK LP +DPR F  QA VHC
Sbjct: 126 KIHDIIDFIHPPHTTKLRIRPPAHSLTPKQQFNFEKAIHIMKRLPPNDPRNFYRQADVHC 185

Query: 181 AYCNYAYKQLDSVPKAELEVHWNALFFPFHRCYLYFFERILGYLIDDPTFAIPFWNWDSP 240
           AYC+YAYKQL   P AE++VHWNALFFPFHR YLYFFERILG+LI++P F IPFWNWD+ 
Sbjct: 186 AYCDYAYKQL-GFPNAEMQVHWNALFFPFHRAYLYFFERILGHLINEPNFVIPFWNWDNT 245

Query: 241 PGMKMPKIYNNPDSSLYDRLRDARHVPPKLAYLNYDGDEDPPFDVVDCNLRWMNKQMKVA 300
            GM+MP+IY++P+S LYD+LR+ RH P KL  LNY GD++P +DVVD NLRWM+KQM   
Sbjct: 246 EGMQMPEIYDHPNSPLYDKLRNQRHRPHKLLNLNYHGDDNPSYDVVDSNLRWMHKQMITD 305

Query: 301 GKSARGFLGQLIRRGEEA-KTAGMGSIESSPHNNLHDWVGDPNSPHNEDMGTFYSAGNDP 360
             +A GF G+L+    EA +  GMG+IE SPHNN+H+WVGDPN+ ++ +MG FYSA  DP
Sbjct: 306 ANTAEGFHGKLLIASHEANEETGMGTIERSPHNNIHNWVGDPNAKNHMNMGAFYSAARDP 365

Query: 361 LFYGHHANVDRMWSIWQKLET-KRRDYTDPDWLDSSFVLYDENKNAVRIKVRDCVDSVNL 420
           LFYGHHANVDRMWSIWQ L+  K RD+ +PDWL+SSFV Y+E+K AVRIKV DC+D+  L
Sbjct: 366 LFYGHHANVDRMWSIWQGLKNGKPRDFDNPDWLNSSFVFYNEHKQAVRIKVSDCLDTRRL 425

Query: 421 GYVYQDVDIQWLNTRQIPRQNPPTSSSPEPPLAPPPEVYFPAMLNSEINTVVGRPSRSRT 480
           GYVYQ VD+ WL+T+Q PR      ++  P +     + FP  LNS + T V RP++   
Sbjct: 426 GYVYQYVDLPWLHTKQTPRLKKQLKTTQAPQV-----IKFPQGLNSMVTTTVKRPNKLMN 485

Query: 481 IEQKGEEDEVLVIEDIEFDRSSRVHFNVLVNVLDEGLDVSPANIEFAGSFRNIPHAHNHN 540
            ++K E+DEVLVIE IE D+   VHFNVLVNV+D+G DV P N EF G+F  +PH HNH 
Sbjct: 486 EKEKSEKDEVLVIEGIELDKRFWVHFNVLVNVVDDGSDVCPGNTEFVGTFTTVPHGHNH- 545

Query: 541 NAKNSLRFVITELIEELGLDDAETLHVKIAPKLGGENVTIGGIKIELQP 587
             K SLR VITE+++ELG++D E++ V++ PK+GG+ VTIGG+KIE QP
Sbjct: 546 RGKTSLRVVITEVLKELGIEDDESVIVRLDPKVGGDKVTIGGMKIEHQP 566

BLAST of MS006102 vs. ExPASy Swiss-Prot
Match: P43309 (Polyphenol oxidase, chloroplastic OS=Malus domestica OX=3750 PE=1 SV=1)

HSP 1 Score: 495.7 bits (1275), Expect = 7.0e-139
Identity = 276/550 (50.18%), Postives = 352/550 (64.00%), Query Frame = 0

Query: 52  SNPDEVGISVNKFDRRDVLFGLGGLYGVASLSGGDRFALADDTHTISPDTANCGPARTPA 111
           +N D+   + +K DRR+VL GLGGLYGVA + G D FA A       PD + CGPA  P 
Sbjct: 51  TNNDQNDQAQSKLDRRNVLLGLGGLYGVAGM-GTDPFAFAKP--IAPPDVSKCGPADLPQ 110

Query: 112 G--PRNCCPPKIHDIINF-IPPPMIPHPRVRPAAHLITPEQLAKFEKAIYLMKHLPYDDP 171
           G  P NCCPP    II+F +P P     R+RP AH +      K+ KA+ LMK LP DDP
Sbjct: 111 GAVPTNCCPPPSTKIIDFKLPAP--AKLRIRPPAHAVDQAYRDKYYKAMELMKALPDDDP 170

Query: 172 RRFSNQAQVHCAYCNYAYKQLDSVPKAELEVHWNALFFPFHRCYLYFFERILGYLIDDPT 231
           R F  QA VHCAYC+ AY Q+   P+ EL++H + LFFPFHR YLYFFE+ILG LI+DPT
Sbjct: 171 RSFKQQAAVHCAYCDGAYDQV-GFPELELQIHNSWLFFPFHRYYLYFFEKILGKLINDPT 230

Query: 232 FAIPFWNWDSPPGMKMPKIYNNPDSSLYDRLRDARHVPPKLAYLNYDGDED--PPFDVVD 291
           FA+PFWNWDSP GM +P IY +P S LYD+LR A H PP L  L+Y+G ED       ++
Sbjct: 231 FALPFWNWDSPAGMPLPAIYADPKSPLYDKLRSANHQPPTLVDLDYNGTEDNVSKETTIN 290

Query: 292 CNLRWMNKQMKVAGKSARGFLGQLIRRGEEAKTAGMGSIESSPHNNLHDWVGDPNSPHNE 351
            NL+ M +QM    K+A+ F G   R G+E    G GSIE +PH  +H W GD   P+ E
Sbjct: 291 ANLKIMYRQMVSNSKNAKLFFGNPYRAGDEPDPGG-GSIEGTPHAPVHLWTGDNTQPNFE 350

Query: 352 DMGTFYSAGNDPLFYGHHANVDRMWSIWQKLETKRRDYTDPDWLDSSFVLYDENKNAVRI 411
           DMG FYSAG DP+F+ HH+NVDRMWSIW+ L  KR D TD DWLDS F+ Y+EN   VR+
Sbjct: 351 DMGNFYSAGRDPIFFAHHSNVDRMWSIWKTLGGKRTDLTDSDWLDSGFLFYNENAELVRV 410

Query: 412 KVRDCVDSVNLGYVYQDVDIQWLNTRQIPRQNPPTSSSPEPPL-------APPPEVY--- 471
           KVRDC+++ NLGYVYQDVDI WL+++  PR+     S     L       A   +V    
Sbjct: 411 KVRDCLETKNLGYVYQDVDIPWLSSKPTPRRAKVALSKVAKKLGVAHAAVASSSKVVAGT 470

Query: 472 -FPAMLNSEINTVVGRP-SRSRTIEQKGEEDEVLVIEDIEFDRSSRVHFNVLVNVLDEGL 531
            FP  L S+I+TVV RP  + R+ + K +E+E+LVIE IEFDR   V F+V VN +D+ L
Sbjct: 471 EFPISLGSKISTVVKRPKQKKRSKKAKEDEEEILVIEGIEFDRDVAVKFDVYVNDVDD-L 530

Query: 532 DVSPANIEFAGSFRNIPHAHNHNNAKNS-LRFVITELIEELGLDDAETLHVKIAPKLGGE 584
              P   EFAGSF ++PH+H H    N+ LR  +T+L+EE+  +D +++ V + PK G  
Sbjct: 531 PSGPDKTEFAGSFVSVPHSHKHKKKMNTILRLGLTDLLEEIEAEDDDSVVVTLVPKFGA- 590

BLAST of MS006102 vs. ExPASy Swiss-Prot
Match: P43311 (Polyphenol oxidase, chloroplastic OS=Vitis vinifera OX=29760 PE=1 SV=1)

HSP 1 Score: 486.1 bits (1250), Expect = 5.6e-136
Identity = 280/598 (46.82%), Postives = 364/598 (60.87%), Query Frame = 0

Query: 1   MASLSSLLSLPPYYHSKTQQSRPQNRRHGVSYHFVPCRASYNNGDETPIIKSNPDEVGIS 60
           +A+ +++ + PP    +     P  R    S  F P + S N+ +  P   S  D    S
Sbjct: 14  IANTTNISAFPPSPLFQRASHVPVARNR--SRRFAPSKVSCNSANGDPNSDSTSDVRETS 73

Query: 61  VNKFDRRDVLFGLGGLYGVASLSGGDRFALADDTHTISPDTANCGPARTPAG--PRNCCP 120
             K DRR+VL G+GGLYG A   G  +  LA      +PD + CG A  P G  P NCCP
Sbjct: 74  SGKLDRRNVLLGIGGLYGAAGGLGATK-PLAFGAPIQAPDISKCGTATVPDGVTPTNCCP 133

Query: 121 PKIHDIINFIPPPMIPHPRVRPAAHLITPEQLAKFEKAIYLMKHLPYDDPRRFSNQAQVH 180
           P    II+F  P      R RPAAHL++ E LAK++KAI L K LP DDPR F  QA VH
Sbjct: 134 PVTTKIIDFQLPSSGSPMRTRPAAHLVSKEYLAKYKKAIELQKALPDDDPRSFKQQANVH 193

Query: 181 CAYCNYAYKQLDSVPKAELEVHWNALFFPFHRCYLYFFERILGYLIDDPTFAIPFWNWDS 240
           C YC  AY Q+      EL+VH + LF PFHR YLYF ERIL  LIDDPTFA+P+W WD+
Sbjct: 194 CTYCQGAYDQV-GYTDLELQVHASWLFLPFHRYYLYFNERILAKLIDDPTFALPYWAWDN 253

Query: 241 PPGMKMPKIYNNPDSSLYDRLRDARHVPPKLAYLNYDGDED--PPFDVVDCNLRWMNKQM 300
           P GM MP IY +  SSLYD  R+A+H+PP +  L+YDG E   P  ++   NL  M KQ+
Sbjct: 254 PDGMYMPTIYASSPSSLYDEKRNAKHLPPTVIDLDYDGTEPTIPDDELKTDNLAIMYKQI 313

Query: 301 KVAGKSARGFLGQLIRRGEEAKTAGMGSIESSPHNNLHDWVGDPNSPHNEDMGTFYSAGN 360
                + + FLG   R G +A   G G++E +PHN +H W G  + P +EDMG FY+AG 
Sbjct: 314 VSGATTPKLFLGYPYRAG-DAIDPGAGTLEHAPHNIVHKWTGLADKP-SEDMGNFYTAGR 373

Query: 361 DPLFYGHHANVDRMWSIWQKLETK-RRDYTDPDWLDSSFVLYDENKNAVRIKVRDCVDSV 420
           DP+F+GHHANVDRMW+IW+ +  K R+D+TD DWLD++FV YDENK  V++KV DCVD+ 
Sbjct: 374 DPIFFGHHANVDRMWNIWKTIGGKNRKDFTDTDWLDATFVFYDENKQLVKVKVSDCVDTS 433

Query: 421 NLGYVYQDVDIQWL--NTRQIPRQNPPTSSSPEPPLAPPPEVY------FPAMLNSEINT 480
            L Y YQD+ I WL  NT+   +    +S S     A  P+        FP  LNS I  
Sbjct: 434 KLRYQYQDIPIPWLPKNTKAKAKTTTKSSKSGVAKAAELPKTTISSIGDFPKALNSVIRV 493

Query: 481 VVGRPSRSRTIEQKGEEDEVLVIEDIEFDRSSRVHFNVLVNVLDEGLDVS-PANIEFAGS 540
            V RP +SR+ ++K +E+EVL+I+ IE DR + V F+V +N  DE   VS P N EFAGS
Sbjct: 494 EVPRPKKSRSKKEKEDEEEVLLIKGIELDRENFVKFDVYIN--DEDYSVSRPKNSEFAGS 553

Query: 541 FRNIPHAH-NHNNAKNSLRFVITELIEELGLDDAETLHVKIAPKLGGENVTIGGIKIE 584
           F N+PH H      K +LRF I EL+E+LG +D E++ V I P+ GG++VTIGGI+IE
Sbjct: 554 FVNVPHKHMKEMKTKTNLRFAINELLEDLGAEDDESVIVTIVPRAGGDDVTIGGIEIE 603

BLAST of MS006102 vs. ExPASy Swiss-Prot
Match: Q6UIL3 ((+)-larreatricin hydroxylase, chloroplastic OS=Larrea tridentata OX=66636 PE=1 SV=1)

HSP 1 Score: 448.0 bits (1151), Expect = 1.7e-124
Identity = 263/599 (43.91%), Postives = 358/599 (59.77%), Query Frame = 0

Query: 1   MASLSS---LLSLPPYYHSKTQQSRPQNRRHGVSYHFVPCRASYNNGDETPIIKSNPDEV 60
           MASLSS   LL+ P  +   T+ SR   RR       V C+AS +N D     K N  E 
Sbjct: 1   MASLSSQSKLLATPYSFPYHTKPSRVSLRR-------VSCKASNDNKD-----KPNDQEK 60

Query: 61  GISVNKFDRRDVLFGLGGLYGVASLSGGDRFALADDTH-TISPDTANCGPARTPAGPRN- 120
             S+   DRR++L GLGGLYG +++   ++  LA      + PD  +C P    A   N 
Sbjct: 61  TFSI---DRRNMLIGLGGLYGASNVFPSNQSTLAAPIQPPVLPD--SCHPPEDLAEGVNV 120

Query: 121 -CCPPKIHDIINFIPPPMIPHPRVRPAAHLITPEQLAKFEKAIYLMKHLPYDDPRRFSNQ 180
            CCPP + D I+F  P      R+RPAAHL  P  + K++KA+  MK LP +DPR F  Q
Sbjct: 121 LCCPPDVKDPIDFQMPSNPSRLRIRPAAHLADPTYIEKYKKALAAMKALPQNDPRSFYQQ 180

Query: 181 AQVHCAYCNYAYKQLDSVPKAELEVHWNALFFPFHRCYLYFFERILGYLIDDPTFAIPFW 240
           A +HCAYCN AY Q+   P   ++VH + LF PFHR YLYF+ERILG LIDDPTFAIPFW
Sbjct: 181 ANIHCAYCNGAYDQV-GFPDVNIQVHHSWLFLPFHRWYLYFYERILGSLIDDPTFAIPFW 240

Query: 241 NWDSPPGMKMPKIYNNPDSSLYDRLRDARHVPPKLAYLNYDGDEDPPFD--VVDCNLRWM 300
           NWD+P GM MP ++ +P+S LYD  R+  H P  +  L++   E P  +   +  NL  M
Sbjct: 241 NWDAPKGMHMPHMFIDPNSPLYDAKRNPAHFPDTIVDLDFSSGEAPSHNPRQIGNNLSIM 300

Query: 301 NKQMKVAGKSARGFLGQLIRRGEEAKTAGMGSIESSPHNNLHDWVGDPNSPHNEDMGTFY 360
            KQ+ V  K AR F G+ ++ G     +G GS+E +PH N+H W GDP   + E+MG FY
Sbjct: 301 YKQV-VRAKKARLFHGRPLQAGSFPDESGDGSLEGTPHGNIHLWSGDPRQSNFENMGNFY 360

Query: 361 SAGNDPLFYGHHANVDRMWSIWQKLETKRRDYTDPDWLDSSFVLYDENKNAVRIKVRDCV 420
           SAG DPLFY HHANVDRMW IW K    R+DY   DWL++ F+L+DEN   VR+ VRD +
Sbjct: 361 SAGRDPLFYAHHANVDRMWYIW-KNSLGRKDYKKKDWLNAGFLLFDENAQPVRVYVRDAL 420

Query: 421 DSVNLGYVYQDVDIQWLNTRQIPRQNPPTSSSPEPPLAPPPEVY--FPAMLNSEINTVVG 480
           D   LGY YQ+VDI W+N++  PR+  P  +          ++   FP  L+S ++  V 
Sbjct: 421 DERKLGYAYQEVDIPWINSKPKPRKANPWPNRLRSKATTTTKLINKFPLTLDSTVSFEVK 480

Query: 481 RPSRSRTIEQKGEEDEVLVIEDIEFDRSSRVHFNVLVNVLDEGLDVSPAN-IEFAGSFRN 540
           RP +SR+  +K +E+EVLVIE I+ +    + F+V +N  DE  D S A+  EFAGSF N
Sbjct: 481 RPKKSRSKSEKEDEEEVLVIEKIKHEPQFPLKFDVYIN--DEDEDPSAADQTEFAGSFVN 540

Query: 541 IPHAHNHNNAKN-----SLRFVITELIEELGLDDAETLHVKIAPKLGGENVTIGGIKIE 584
           +PH H H + K+     +L   I+E+++EL +D  +++ V + P++G   +TIGG KIE
Sbjct: 541 VPHFHRHGDKKDKRQTTNLSIGISEVLDELDVDGDDSIVVTLVPRVGSGQITIGGAKIE 577

BLAST of MS006102 vs. ExPASy Swiss-Prot
Match: O81103 (Polyphenol oxidase latent form, chloroplastic OS=Prunus armeniaca OX=36596 GN=PPO PE=1 SV=1)

HSP 1 Score: 444.1 bits (1141), Expect = 2.4e-123
Identity = 262/593 (44.18%), Postives = 343/593 (57.84%), Query Frame = 0

Query: 2   ASLSSLLSLPPYYHSKTQQSRPQNRRHGVSYH-FVPCRASYNNGDETPIIKSNPDEVGIS 61
           +SL S  S   +  +K+Q S     +H V+    + C+A+ NN           +E    
Sbjct: 14  SSLISTNSFSTFLPNKSQLSLSGKSKHYVARRSSISCKATNNNNSNNQ--NEQQEESSRL 73

Query: 62  VNKFDRRDVLFGLGGLYGVASLSGGDR--FALADDTHTISPDTANCGPAR-TPAGPRN-- 121
           + K DRR++L GLGGLYG  +L   DR  FA AD      PD   C PA  TP G     
Sbjct: 74  LGKLDRRNILIGLGGLYGATTL---DRKPFAFADP--IAPPDLTTCKPAEITPGGSETVP 133

Query: 122 CCPPKIHDIINFIPPPMIPHPRVRPAAHLITPEQLAKFEKAIYLMKHLPYDDPRRFSNQA 181
           CCPP    I  F P   IP  R  PAAH +T E LAKF+KA   M+ LP DDPR    QA
Sbjct: 134 CCPPVTTKIKTFKPDLSIP-LRTSPAAHQVTDEYLAKFKKAQAAMRALPDDDPRSMVQQA 193

Query: 182 QVHCAYCNYAYKQLDSVPKAELEVHWNALFFPFHRCYLYFFERILGYLIDDPTFAIPFWN 241
           +VHCAYCN AY Q+      +++VH++ LFFPFHR YLYF+ERILG LIDDPTFA+P+WN
Sbjct: 194 KVHCAYCNGAYPQVGFTDN-DIQVHFSWLFFPFHRMYLYFYERILGKLIDDPTFALPYWN 253

Query: 242 WDSPPGMKMPKIYNNPDSSLYDRLRDARHVPPKLAYLNYDGDEDPPFDV--VDCNLRWMN 301
           WDSP G  +P IY +  S LYD+ R+A H PP L  L+Y G +D   +   +D NL  M 
Sbjct: 254 WDSPVGFPIPDIYTDTSSPLYDQYRNADHQPPVLVDLSYGGKDDDVDEQTRIDENLAIMY 313

Query: 302 KQMKVAGKSARGFLGQLIRRGEEAKTAGMGSIESSPHNNLHDWVGDPNSPHNEDMGTFYS 361
           +QM    K+   F G   R G        G+IE+ PHNN+H WVGDP+  H EDMG FYS
Sbjct: 314 RQMVSGAKTPDLFFGHAYRAGNLNTGKYPGTIENMPHNNIHIWVGDPSQTHQEDMGNFYS 373

Query: 362 AGNDPLFYGHHANVDRMWSIWQKLETKRRDYTDPDWLDSSFVLYDENKNAVRIKVRDCVD 421
           AG DPLFY HHANVDRMW+IW+ L  KR+D TD DWLD+ F+ YDEN   VR+KVRD ++
Sbjct: 374 AGRDPLFYAHHANVDRMWNIWKTLGGKRKDITDTDWLDAEFLFYDENAELVRVKVRDSLE 433

Query: 422 -SVNLGYVYQDVDIQWLNTRQIPRQNPPTSSSPEPPLAPPPEVYFPAMLNSEINTVVGRP 481
               L Y Y+ V + WL T+  P      + +     A      FPA L       V RP
Sbjct: 434 PEKQLRYNYEPVSLPWLFTK--PTARKTKNKTKAKVAATQLTSKFPATLVEVTTVEVARP 493

Query: 482 S-RSRTIEQKGEEDEVLVIEDIEFDRSSRVHFNVLVNVLDEGLDVSPANIEFAGSFRNIP 541
             R R+ ++K +E+E+L+I+DIEF+ +  V F+V +N   E L     + EFAGSF ++P
Sbjct: 494 KPRKRSKKEKVDEEELLIIKDIEFEGTEAVKFDVFINDDAESLSRRDKS-EFAGSFVHVP 553

Query: 542 HAHNHN-NAKNSLRFVITELIEELGLDDAETLHVKIAPKLGGENVTIGGIKIE 584
                    K +L+  IT+L+E+LG +D  ++ V + P++    +TIGG KIE
Sbjct: 554 QGKTTKAKTKTNLKLGITDLLEDLGAEDDSSVLVTLVPRVSNSPITIGGFKIE 594

BLAST of MS006102 vs. ExPASy Swiss-Prot
Match: Q06215 (Polyphenol oxidase A1, chloroplastic OS=Vicia faba OX=3906 PE=1 SV=1)

HSP 1 Score: 436.4 bits (1121), Expect = 5.1e-121
Identity = 261/600 (43.50%), Postives = 352/600 (58.67%), Query Frame = 0

Query: 9   SLPPYYHSKTQQSRPQNRRHGVSYHFVPCRASYNNGDETPIIKSNPDEVGISVNKFDRRD 68
           S+ P+   + Q S+ +  +  V+        S NN    P      +E  +S     RR+
Sbjct: 25  SVYPFLQKQHQSSKLRKPKRQVT-------CSSNNNQNNP-----KEEQELSNIVGHRRN 84

Query: 69  VLFGLGGLYGVASLSGGDRFALADDTHTISPDTANC-GPARTPAGPR----NCCPPKIHD 128
           VL GLGG+YG  + +     ALA       PD + C  P+  P+G      NCCPP    
Sbjct: 85  VLIGLGGIYGTLATNPS---ALASPIS--PPDLSKCVPPSDLPSGTTPPNINCCPPYSTK 144

Query: 129 IINFIPPPMIPHPRVRPAAHLITPEQLAKFEKAIYLMKHLPYDDPRRFSNQAQVHCAYCN 188
           I +F  P   P  RVR AAHL+  E L K++KA  LMK LP +DPR F+ QA +HCAYC+
Sbjct: 145 ITDFKFPSNQP-LRVRQAAHLVDNEFLEKYKKATELMKALPSNDPRNFTQQANIHCAYCD 204

Query: 189 YAYKQLDSVPKAELEVHWNALFFPFHRCYLYFFERILGYLIDDPTFAIPFWNWDSPPGMK 248
            AY Q+   P  +L+VH + LFFPFHR YLYF+ERILG LI+DPTFA+PFWN+D+P GM+
Sbjct: 205 GAYSQI-GFPDLKLQVHGSWLFFPFHRWYLYFYERILGSLINDPTFALPFWNYDAPDGMQ 264

Query: 249 MPKIYNNPDSSLYDRLRDARHVPPKLAYLNY--DGDEDPPFDVVDCNLRWMNKQMKVAGK 308
           +P IY +  S LYD LR+A H PP L  LN+   G +    +++  NL  M +Q+   GK
Sbjct: 265 LPTIYADKASPLYDELRNASHQPPTLIDLNFCDIGSDIDRNELIKTNLSIMYRQVYSNGK 324

Query: 309 SARGFLGQLIRRGEEAKTAGMGSIESSPHNNLHDWVGDPNSPHNEDMGTFYSAGNDPLFY 368
           ++R FLG   R G +A+  G GSIE+ PH  +H W GD    + EDMG FYSA  DP+FY
Sbjct: 325 TSRLFLGNPYRAG-DAEPQGAGSIENVPHAPVHTWTGDNTQTNIEDMGIFYSAARDPIFY 384

Query: 369 GHHANVDRMWSIWQKLETKRRDYTDPDWLDSSFVLYDENKNAVRIKVRDCVDSVNLGYVY 428
            HH+NVDR+W IW+ L  K+ D+TD DWL+S F+ YDENKN VR+ V+D +D   LGY Y
Sbjct: 385 SHHSNVDRLWYIWKTLGGKKHDFTDKDWLESGFLFYDENKNLVRVNVKDSLDIDKLGYAY 444

Query: 429 QDVDIQWLNTRQIPRQNPPTS------------SSPEPPL----APPPEVYFPAMLNSEI 488
           QDV I W   + +PR+                  SP   L    +P   V FP +LN+++
Sbjct: 445 QDVPIPWEKAKPVPRRTKVQKLVEVEVNDGNLRKSPTIFLVRQQSPRKYVTFPLVLNNKV 504

Query: 489 NTVVGRPSRSRTIEQKGEEDEVLVIEDIEFDRSSRVHFNVLVNVLDEGLDVSPANIEFAG 548
           + +V RP + R+ ++K EE+EVLVIE IEF  +  + F+V +N  DE   V   N EFAG
Sbjct: 505 SAIVKRPKKLRSKKEKEEEEEVLVIEGIEFYMNIAIKFDVYIN--DEDDKVGAGNTEFAG 564

Query: 549 SFRNIPH-AHNHNNAK--NSLRFVITELIEELGLDDAETLHVKIAPKLGGENVTIGGIKI 583
           SF NIPH AH H N K   SLR  IT+L+E+L ++  + + V + PK G   V I  ++I
Sbjct: 565 SFVNIPHSAHGHKNKKIITSLRLGITDLLEDLHVEGDDNIVVTLVPKCGSGQVKINNVEI 602

BLAST of MS006102 vs. ExPASy TrEMBL
Match: A0A172DC01 (Polyphenol oxidase OS=Luffa aegyptiaca OX=3670 GN=PPO2 PE=2 SV=1)

HSP 1 Score: 673.3 bits (1736), Expect = 9.1e-190
Identity = 368/592 (62.16%), Postives = 426/592 (71.96%), Query Frame = 0

Query: 1   MASLSSLLSLPPYYHSKTQQSRPQNRRHGVSYHFVPCRASYNNGDETPIIKSNPDEVGIS 60
           MASLSSLLSL P   SK Q S   NRR G+SY  +PCRAS  NG +TP + SNP E G S
Sbjct: 1   MASLSSLLSLFPCIRSKNQSSSRGNRRLGLSY--IPCRAS--NGVQTP-MNSNPQE-GDS 60

Query: 61  VNKFDRRDVLFGLGGLYGVASLSGGDRFALADDTHTISPDTANCGPARTPAGPR-NCCPP 120
           ++KFDRRDVLFGLGGLY  A+L G DR ALAD    ISPD  +C P    A  + +CCPP
Sbjct: 61  MSKFDRRDVLFGLGGLYSAAAL-GRDRSALAD---PISPDVIHCEPITLEADEKVDCCPP 120

Query: 121 KIHDIINFIPPPMIPHPRVRPAAHLITPEQLAKFEKAIYLMKHLPYDDPRRFSNQAQVHC 180
            I DI +F+ PP + + RVRPAAHLIT EQLAK E+A+  MK L  DDPR F  QA+VHC
Sbjct: 121 SILDITDFV-PPAVENLRVRPAAHLITQEQLAKLEEAVTKMKALDPDDPRNFYQQARVHC 180

Query: 181 AYCNYAYKQLDSVPKAELEVHWNALFFPFHRCYLYFFERILGYLIDDPTFAIPFWNWDSP 240
           AYCN AYKQ D  P+ + ++HWN LFFPFHRCYLYF ERILG LI+DP FAIPFWNWD+ 
Sbjct: 181 AYCNDAYKQ-DGFPELDFDIHWNWLFFPFHRCYLYFHERILGSLINDPDFAIPFWNWDAI 240

Query: 241 PGMKMPKIYNNPD--SSLYDRLRDARHVPPKLAYLNYDG-DEDPPFDVVDCNLRWMNKQM 300
           PGM+MP IY N D  S LYD LRD  H PPKL  LNYDG  EDP +DV+ CN R M +QM
Sbjct: 241 PGMQMPAIYINDDKNSPLYDELRDPNHKPPKLLDLNYDGATEDPSYDVLFCNPRLMYRQM 300

Query: 301 KVAGKSARGFLGQLIRRGEEAKTAGMGSIESSPHNNLHDWVGDPNSPHNEDMGTFYSAGN 360
            +  K+A GF G+ +R G+EA   GMGSIE S HNN+H W GDP   + EDMG FY+A  
Sbjct: 301 VINSKTAEGFHGKPLRAGQEAYRTGMGSIELSAHNNVHTWSGDPQQKYREDMGHFYAAAR 360

Query: 361 DPLFYGHHANVDRMWSIWQKL-ETKRRDYTDPDWLDSSFVLYDENKNAVRIKVRDCVDSV 420
           DPLFY HHANVDRMW+IWQ L + K RD+TDPDWL++SFV YDENKNA+RIKV+DC+D+ 
Sbjct: 361 DPLFYAHHANVDRMWTIWQGLIDGKSRDFTDPDWLNASFVFYDENKNAIRIKVQDCLDNE 420

Query: 421 NLGYVYQDVDIQWLNTRQIPRQNPPTSSSPEPPLAPPPEVYFPAMLNSEINTVVGRPSRS 480
            LGY YQ V+I WL+T+ IPRQ      S +    PP EV     LNS++   V RP RS
Sbjct: 421 KLGYKYQAVEIPWLHTKPIPRQKTSQLKSSKAQETPPIEV-----LNSKMTKEVKRPQRS 480

Query: 481 RTIEQKGEEDEVLVIEDIEFDRSSRVHFNVLVNVLDEGLDVSPANIEFAGSFRNIPHAHN 540
           R+  QK  EDEVLVIE +E D +SRVHFNVLVNVLD+G DVSP N EFAGSF++IP  H 
Sbjct: 481 RSKTQKSVEDEVLVIE-MELDTTSRVHFNVLVNVLDDGTDVSPGNTEFAGSFKSIPLGHK 540

Query: 541 HNNAKNSLRFVITELIEELGLDDAETLHVKIAPKLGGENVTIGGIKIELQPI 588
           H  A  SL  VITELIEELG DD ET+ V I PKLGG+ VTIGGIKIELQPI
Sbjct: 541 H-RANTSLSLVITELIEELGYDDDETVFVTIDPKLGGDLVTIGGIKIELQPI 573

BLAST of MS006102 vs. ExPASy TrEMBL
Match: A0A6J1K2N9 (polyphenol oxidase, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111490108 PE=3 SV=1)

HSP 1 Score: 660.6 bits (1703), Expect = 6.1e-186
Identity = 338/590 (57.29%), Postives = 423/590 (71.69%), Query Frame = 0

Query: 1   MASLSSLLSLPPYYHSKTQQSRPQNRRHGVSYHFVPCRASYNNGDETPIIKSNPDEVGIS 60
           MAS+S LLS+ PY   K Q S  QN+  G+++  VPCRAS  NGD T             
Sbjct: 1   MASISPLLSILPYSQIKNQTSNRQNKSRGINH--VPCRAS--NGDST------------- 60

Query: 61  VNKFDRRDVLFGLGGLYGVASLSGGDRFALADDTHTISPDTANCGPARTPAGPRN--CCP 120
            +KFDRRDVLFGLGGLYG A+L+     A A     ++PD  NC PA T  G  +  CCP
Sbjct: 61  -SKFDRRDVLFGLGGLYGAAALTNQQSPASA---KPLAPDVNNCEPA-TIVGNEHIPCCP 120

Query: 121 PKIHDIINFIPPPMIPHPRVRPAAHLITPEQLAKFEKAIYLMKHLPYDDPRRFSNQAQVH 180
           PKIHDII+FI PP     R+R  AH +TP+Q   FEKAI++MK L  +DPR F  QA VH
Sbjct: 121 PKIHDIIDFIHPPHTTKLRIRQPAHSLTPKQQFNFEKAIHIMKRLSPNDPRNFYRQADVH 180

Query: 181 CAYCNYAYKQLDSVPKAELEVHWNALFFPFHRCYLYFFERILGYLIDDPTFAIPFWNWDS 240
           CAYC+YAYKQL   P AE++VHWNALFFPFHR YLYFFERILG+LI++P FAIPFWNWD+
Sbjct: 181 CAYCDYAYKQL-GFPNAEMQVHWNALFFPFHRAYLYFFERILGHLINEPDFAIPFWNWDN 240

Query: 241 PPGMKMPKIYNNPDSSLYDRLRDARHVPPKLAYLNYDGDEDPPFDVVDCNLRWMNKQMKV 300
             GM+MP+IY++P+S LYD+LR+ RH P KL  LNY GD++PP+DVVDCNLRWM+KQM  
Sbjct: 241 KKGMQMPEIYDHPNSPLYDKLRNQRHRPHKLLNLNYHGDDNPPYDVVDCNLRWMHKQMIT 300

Query: 301 AGKSARGFLGQLIRRGEEA-KTAGMGSIESSPHNNLHDWVGDPNSPHNEDMGTFYSAGND 360
              +A GF G+L+   +EA +  GMG+IE SPHNN+H+WVGDPN+ +N +MG FYSA  D
Sbjct: 301 DADTAEGFHGKLLVASQEANEETGMGTIERSPHNNIHNWVGDPNAKNNMNMGVFYSAARD 360

Query: 361 PLFYGHHANVDRMWSIWQKLET-KRRDYTDPDWLDSSFVLYDENKNAVRIKVRDCVDSVN 420
           PLFYGHHANVDRMWSIWQ L+  K RD+ +PDWL+SSFV Y+E+K AVRIKV DC+D+  
Sbjct: 361 PLFYGHHANVDRMWSIWQGLKNGKPRDFNNPDWLNSSFVFYNEHKQAVRIKVSDCLDTRR 420

Query: 421 LGYVYQDVDIQWLNTRQIPRQNPPTSSSPEPPLAPPPEVYFPAMLNSEINTVVGRPSRSR 480
           LGYVYQ VD+ WL+T+Q PR      ++  P +     + FP  LNS + T V RP + R
Sbjct: 421 LGYVYQYVDLPWLHTKQTPRLKKQLKTTQAPQV-----IKFPQGLNSMVTTTVKRPKKLR 480

Query: 481 TIEQKGEEDEVLVIEDIEFDRSSRVHFNVLVNVLDEGLDVSPANIEFAGSFRNIPHAHNH 540
             ++K E+DEVLVIE IE D+   VHFNVLVNV+D+G DV P N EF G+F  + H HNH
Sbjct: 481 DEKEKSEKDEVLVIEGIELDKRYWVHFNVLVNVVDDGSDVCPGNTEFVGTFTTVLHGHNH 540

Query: 541 NNAKNSLRFVITELIEELGLDDAETLHVKIAPKLGGENVTIGGIKIELQP 587
              K SLR VITE+++ELG++D E++ V++ PK+GG+ VTIGG+KIELQP
Sbjct: 541 -RGKTSLRVVITEVLKELGIEDDESVIVRLDPKVGGDKVTIGGMKIELQP 561

BLAST of MS006102 vs. ExPASy TrEMBL
Match: A0A6J1GRK5 (polyphenol oxidase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111456826 PE=3 SV=1)

HSP 1 Score: 656.8 bits (1693), Expect = 8.8e-185
Identity = 334/589 (56.71%), Postives = 418/589 (70.97%), Query Frame = 0

Query: 1   MASLSSLLSLPPYYHSKTQQSRPQNRRHGVSYHFVPCRASYNNGDETPIIKSNPDEVGIS 60
           MAS+S LLS+ PY   K Q S  QN+  G++   VPCRAS  NGD T             
Sbjct: 1   MASISPLLSILPYSQIKNQTSNLQNKSRGINQ--VPCRAS--NGDST------------- 60

Query: 61  VNKFDRRDVLFGLGGLYGVASLSGGDRFALADDTHTISPDTANCGPARTPAGPR-NCCPP 120
            +KFDRRDVLFGLGGLYG A+L+     A A     ++PD  NC  A         CCPP
Sbjct: 61  -SKFDRRDVLFGLGGLYGAAALTNQQSPASA---APLAPDVNNCEAATIEGNEHIPCCPP 120

Query: 121 KIHDIINFIPPPMIPHPRVRPAAHLITPEQLAKFEKAIYLMKHLPYDDPRRFSNQAQVHC 180
           KIHDII+FI PP     R+RP AH +TP+Q   FEKAI++MK LP +DPR F  QA VHC
Sbjct: 121 KIHDIIDFIHPPHTTKLRIRPPAHSLTPKQQFNFEKAIHIMKRLPPNDPRNFYRQADVHC 180

Query: 181 AYCNYAYKQLDSVPKAELEVHWNALFFPFHRCYLYFFERILGYLIDDPTFAIPFWNWDSP 240
           AYC+YAYKQL   P AE++VHWNALFFPFHR YLYFFERILG+LI++P F IPFWNWD+ 
Sbjct: 181 AYCDYAYKQL-GFPNAEMQVHWNALFFPFHRAYLYFFERILGHLINEPNFVIPFWNWDNT 240

Query: 241 PGMKMPKIYNNPDSSLYDRLRDARHVPPKLAYLNYDGDEDPPFDVVDCNLRWMNKQMKVA 300
            GM+MP+IY++P+S LYD+LR+ RH P KL  LNY GD++P +DVVD NLRWM+KQM   
Sbjct: 241 EGMQMPEIYDHPNSPLYDKLRNQRHRPHKLLNLNYHGDDNPSYDVVDSNLRWMHKQMITD 300

Query: 301 GKSARGFLGQLIRRGEEA-KTAGMGSIESSPHNNLHDWVGDPNSPHNEDMGTFYSAGNDP 360
             +A GF G+L+    EA +  GMG+IE SPHNN+H+WVGDPN+ ++ +MG FYSA  DP
Sbjct: 301 ANTAEGFHGKLLIASHEANEETGMGTIEKSPHNNIHNWVGDPNAKNHMNMGAFYSAARDP 360

Query: 361 LFYGHHANVDRMWSIWQKLET-KRRDYTDPDWLDSSFVLYDENKNAVRIKVRDCVDSVNL 420
           LFYGHHANVDRMWSIWQ L+  K RD+ +PDWL+SSFV Y+E+K AVRIKVRDC+D+  L
Sbjct: 361 LFYGHHANVDRMWSIWQGLKNGKARDFDNPDWLNSSFVFYNEHKQAVRIKVRDCLDTRRL 420

Query: 421 GYVYQDVDIQWLNTRQIPRQNPPTSSSPEPPLAPPPEVYFPAMLNSEINTVVGRPSRSRT 480
           GYVYQ VD+ WL+T+Q PR      ++  P +     + FP  LNS + T V RP++   
Sbjct: 421 GYVYQYVDLPWLHTKQTPRLKKQLKTTQAPQV-----IKFPQGLNSMVTTTVKRPNKLMN 480

Query: 481 IEQKGEEDEVLVIEDIEFDRSSRVHFNVLVNVLDEGLDVSPANIEFAGSFRNIPHAHNHN 540
            ++K E+DEVLVIE IE D+   VHFNVLVNV+D+G DV P N EF G+F  +PH HNH 
Sbjct: 481 EKEKSEKDEVLVIEGIELDKRFWVHFNVLVNVVDDGSDVCPGNTEFVGTFTTVPHGHNH- 540

Query: 541 NAKNSLRFVITELIEELGLDDAETLHVKIAPKLGGENVTIGGIKIELQP 587
             K SLR VITE+++ELG++D E++ V++ PK+GG+ VTIGG+KIELQP
Sbjct: 541 RGKTSLRVVITEVLKELGIEDDESVIVRLDPKVGGDKVTIGGMKIELQP 561

BLAST of MS006102 vs. ExPASy TrEMBL
Match: A0A5A7UM88 (Polyphenol oxidase I OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold861G00520 PE=3 SV=1)

HSP 1 Score: 640.2 bits (1650), Expect = 8.5e-180
Identity = 333/591 (56.35%), Postives = 420/591 (71.07%), Query Frame = 0

Query: 1   MASLSSLLSLPPYYHSKTQQSRPQNRRHGVSYHFVPCR-ASYNNGDETPIIKSNPDEVGI 60
           MASLSSLL L PY  +KT+ S   N+RH V  ++V C+ AS NNG++             
Sbjct: 1   MASLSSLLLLSPYSQTKTKFSISSNKRH-VVVNYVSCKAASSNNGED------------- 60

Query: 61  SVNKFDRRDVLFGLGGLYGVASLSGGDRFALADDTHTISPDTANCGPARTPAGPRNCCPP 120
           SV+KFDRR+VLFGLGGLYGV + +G    A       ++PD  NC  A+TP G +NCCPP
Sbjct: 61  SVSKFDRREVLFGLGGLYGVVTATGSVLAA------PLTPDVFNCDMAQTPGGIKNCCPP 120

Query: 121 KIHDIINFIPPPMIPHPRV-RPAAHLITPEQLAKFEKAIYLMKHLPYDDPRRFSNQAQVH 180
           +I  II+F+ PP I  P V RPAAHL+   Q+ + E+ I  +K L  DDP  FS+QA++H
Sbjct: 121 RIGKIIHFV-PPKIQQPYVRRPAAHLMNQTQIEELERGIQKLKQLDEDDPHSFSHQAKIH 180

Query: 181 CAYCNYAYKQLDSVPKAELEVHWNALFFPFHRCYLYFFERILGYLIDDPTFAIPFWNWDS 240
           CAYC+YAY QL S+ +   EVH NALFFPFHR YLYFFERIL + I +P FAIPFWNWDS
Sbjct: 181 CAYCDYAYNQLGSITETRFEVHSNALFFPFHRAYLYFFERILRHYIGNPDFAIPFWNWDS 240

Query: 241 PPGMKMPKIYNNPDSSLYDRLRDARHVPPKLAYLNYDG-DEDPPFDVVDCNLRWMNKQMK 300
           PPGM MP+ YN P SSLYD LRD RH P KL  LNY+G  ED  FDVVDCNLRWMN +MK
Sbjct: 241 PPGMHMPEFYNRPSSSLYDNLRDPRHDPHKLIDLNYNGKTEDHSFDVVDCNLRWMNDRMK 300

Query: 301 VAGKSARGFLGQLIRRGEEAKTAGMGSIESSPHNNLHDWVGDPNSPHNEDMGTFYSAGND 360
           V  +S R FLG++++ GE  K + MG+IE SPHNN+HDWVG   +PH EDMG+FYSA  D
Sbjct: 301 V--QSPRSFLGRVLKAGETPKPSDMGTIEQSPHNNIHDWVG-TKTPHFEDMGSFYSAALD 360

Query: 361 PLFYGHHANVDRMWSIWQKLETKRRDYTDPDWLDSSFVLYDENKNAVRIKVRDCVDSVNL 420
           PLFY HH N+DR+W+IW+ L+   +D+TDPDWL+SSFV YDENKNAVRI+V DC+D+  L
Sbjct: 361 PLFYAHHTNIDRLWNIWKTLDGNPKDHTDPDWLNSSFVFYDENKNAVRIEVSDCLDTEKL 420

Query: 421 GYVYQDVDIQWLNTRQIPRQNP-PTSSSPEPPLAPPPEVYFPAMLNSEINTVVGRPSRSR 480
           GYVYQ+V + WL   +IP  NP   +  PEPP+     + FP  LNS+I T+V RP  + 
Sbjct: 421 GYVYQEVPLPWLLLNKIPSPNPWIRTEEPEPPI-----INFPQTLNSDITTMVDRPEEAG 480

Query: 481 TIEQKGEEDEVLVIEDIEFDRSSRVHFNVLVNVLDEGLDVSPANIEFAGSFRNIPHAHNH 540
           + E   E D VLV+ED+E+D ++RVHF+VLVNV+DE  D   A +EF GSF+NIPH  +H
Sbjct: 481 SGE---EVDVVLVLEDVEYDPTTRVHFDVLVNVVDEAQDRCAAFLEFVGSFKNIPHGLHH 540

Query: 541 NNAKNSLRFVITELIEELGLDDAETLHVKIAPKLGGENVTIGGIKIELQPI 588
           ++ K SLRFVITE+I+ LGL+  + L VKI P +GGE VT+GGIKI+L+P+
Sbjct: 541 HD-KTSLRFVITEVIQGLGLERDKNLAVKIEPMVGGEGVTLGGIKIQLEPV 558

BLAST of MS006102 vs. ExPASy TrEMBL
Match: A0A0A0LUI1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G073090 PE=3 SV=1)

HSP 1 Score: 626.7 bits (1615), Expect = 9.7e-176
Identity = 323/589 (54.84%), Postives = 412/589 (69.95%), Query Frame = 0

Query: 1   MASLSSLLSLPPYYHSKTQQSRPQNRRHGVSYHFVPCRASYNNGDETPIIKSNPDEVGIS 60
           MASLSSLL L  Y  +KT+ S   N+RH V  ++V C+A+ NNG++             S
Sbjct: 1   MASLSSLLLLSSYSQTKTKSSISSNKRH-VVVNYVSCKAASNNGED-------------S 60

Query: 61  VNKFDRRDVLFGLGGLYGVASLSGGDRFALADDTHTISPDTANCGPARTPAGPRNCCPPK 120
           V+KFDRR+VLFGLGGLYGV + +     A       ++PD  NC  A+TP G ++CCPP+
Sbjct: 61  VSKFDRREVLFGLGGLYGVVTATSSVLAA------PLTPDIFNCHMAKTPGGIKDCCPPQ 120

Query: 121 IHDIINFIPPPMIPHPRV-RPAAHLITPEQLAKFEKAIYLMKHLPYDDPRRFSNQAQVHC 180
           I  II+F+ PP I  P V RPAAHL+   Q+ + E  I  +K L   DP  F +QA++HC
Sbjct: 121 IGKIIHFV-PPKIQQPYVRRPAAHLMNQTQIEELESGIKKLKQLDEGDPHSFLHQAKIHC 180

Query: 181 AYCNYAYKQLDSVPKAELEVHWNALFFPFHRCYLYFFERILGYLIDDPTFAIPFWNWDSP 240
           AYC+Y Y QL S+ +    VH NALFFPFHR YLYFFERIL + I +P FAIPFWNWDSP
Sbjct: 181 AYCDYVYNQLGSITETRFGVHSNALFFPFHRAYLYFFERILRHYIGNPNFAIPFWNWDSP 240

Query: 241 PGMKMPKIYNNPDSSLYDRLRDARHVPPKLAYLNYDG-DEDPPFDVVDCNLRWMNKQMKV 300
           PGM MP+ YN P SSLYD LRD RH P KL YLNY+G  ED  FDVVDCNLRWMN +M+V
Sbjct: 241 PGMYMPEFYNRPSSSLYDHLRDPRHDPHKLIYLNYNGKTEDHSFDVVDCNLRWMNDRMQV 300

Query: 301 AGKSARGFLGQLIRRGEEAKTAGMGSIESSPHNNLHDWVGDPNSPHNEDMGTFYSAGNDP 360
             +S R FLG++++ GE      MG+IE SPHNN+HDW+G   +PH EDMG+FYSA  DP
Sbjct: 301 --QSPRSFLGRVLKAGETPNPTDMGTIEQSPHNNIHDWLG-TKTPHFEDMGSFYSAALDP 360

Query: 361 LFYGHHANVDRMWSIWQKLETKRRDYTDPDWLDSSFVLYDENKNAVRIKVRDCVDSVNLG 420
           LFY HHAN+DR+W+IW+ L+   +D+ DPDWL+SSFV YDENKNAV IKV DC+D+  LG
Sbjct: 361 LFYAHHANIDRLWNIWKTLDGNPKDHIDPDWLNSSFVFYDENKNAVNIKVSDCLDTEKLG 420

Query: 421 YVYQDVDIQWLNTRQIPRQNPPTSSSPEPPLAPPPEVYFPAMLNSEINTVVGRPSRSRTI 480
           YVYQ+V + WL+  +IP  NP   +      + PP V FP +LNS+I T+V RP   R+ 
Sbjct: 421 YVYQEVPLPWLHMNKIPTPNPWIRTEE----SEPPIVNFPQILNSDITTMVDRPEEDRSG 480

Query: 481 EQKGEEDEVLVIEDIEFDRSSRVHFNVLVNVLDEGLDVSPANIEFAGSFRNIPHAHNHNN 540
           E   E DEVLV+ED+E+D ++RVHF+VLVNV+D+  D   A +EF GSF+NIPH  +H++
Sbjct: 481 E---EVDEVLVLEDVEYDPTTRVHFDVLVNVVDKAQDRCAAFLEFVGSFKNIPHGLHHHD 540

Query: 541 AKNSLRFVITELIEELGLDDAETLHVKIAPKLGGENVTIGGIKIELQPI 588
            K SLRFVITE+I+ LGL+  + L VKI P +GGE VT+GGIKI+L+P+
Sbjct: 541 -KTSLRFVITEVIQGLGLERDKNLAVKIEPMVGGEGVTVGGIKIQLEPL 557