MS006101 (gene) Bitter gourd (TR) v1

BLAST of MS006101 vs. NCBI nr
Match: KAG6573251.1 (hypothetical protein SDJN03_27138, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 672.5 bits (1734), Expect = 3.1e-189
Identity = 339/576 (58.85%), Postives = 425/576 (73.78%), Query Frame = 0

Query: 7   PYSRTKNQPSSLRNQRQGINYCVVPCRASNNGDQTPINSNPREAKISLNMFDRRDVLFGL 66
           PYS+ KNQ S+L+N+ +GIN+  VPCRAS NGD T             + FDRRDVLFGL
Sbjct: 12  PYSQIKNQTSNLQNKSRGINH--VPCRAS-NGDST-------------SKFDRRDVLFGL 71

Query: 67  GGLYSAATLGGNSS-ASASPQIPDVTICHQAIIEKPRTVDCCPRPPFQADEITHFVLPPA 126
           GGLY AA L    S ASA+P  PDV  C  A IE  + + CCP    +   I  F+ PP 
Sbjct: 72  GGLYGAAALTNQQSPASAAPLAPDVNNCEPATIEGNQHIPCCPP---KIHHIIDFIHPPH 131

Query: 127 TGKLRVRPAAHLMTPHQVAQFEKAIHIMKNCLDKDDPRHFCQQANVHCAYCNFAYKQEKY 186
           T KLR+RP AH +TP Q   FEKAIHIMK  L  +DPR+F +QA+VHCAYC++AYKQ  +
Sbjct: 132 TTKLRIRPPAHSLTPKQQFNFEKAIHIMKR-LPPNDPRNFYRQADVHCAYCDYAYKQLGF 191

Query: 187 -DVDFEVHWNALFFPFHRAYLYFFERICGHLLGDPDFAMPFWNWDSKPGMYFPQIYNDPN 246
            + + +VHWNALFFPFHRAYLYFFERI GHL+ +P+F +PFWNWD+  GM  P+IY+ PN
Sbjct: 192 PNAEMQVHWNALFFPFHRAYLYFFERILGHLINEPNFVIPFWNWDNTEGMQMPEIYDHPN 251

Query: 247 SPLYDKLRDARHTSGKLLDLNYNPNNEDPSYDVADCNVRWMNKQVLVNGNTAEGFLGKLL 306
           SPLYDKLR+ RH   KLL+LNY+  +++PSYDV D N+RWM+KQ++ + +TAEGFLGKLL
Sbjct: 252 SPLYDKLRNQRHRPHKLLNLNYH-GDDNPSYDVVDSNLRWMHKQMIKDADTAEGFLGKLL 311

Query: 307 RGGDEA-EGKGMGSIERSPHNNVHDWIGDPPPNTKYGEDMGNFYSAGRDPVFYAHHGNVD 366
              DEA E  GMG+IERSPHNN+H+W+GD  PN K   +MG FYSA RDP+FY HH NVD
Sbjct: 312 VASDEANEETGMGTIERSPHNNIHNWVGD--PNAKNHMNMGAFYSAARDPLFYGHHANVD 371

Query: 367 RMWSIWRGLTD-VPRDYTDPDWLDSSFVFYDEHKKAVRITVRQCLESKDLGYVYQDVDIP 426
           RMWSIW+GL +  PRD+ +PDWL+SSFVFY+EHK+AVRI VR CL+++ LGYVYQ VD+P
Sbjct: 372 RMWSIWQGLKNGKPRDFDNPDWLNSSFVFYNEHKQAVRIKVRDCLDTRRLGYVYQYVDLP 431

Query: 427 WLYEKQIPRQRREALSAAGAPQVVSFPLSLDSQINASVERTPWI--GKDMGGADEVLVIE 486
           WL+ KQ PR +++ L    APQV+ FP  L+S +  +V+R   +   K+    DEVLVIE
Sbjct: 432 WLHTKQTPRLKKQ-LKTTQAPQVIKFPQGLNSMVTTTVKRPNKLMNEKEKSEKDEVLVIE 491

Query: 487 DIEFDSDSRVHFNVLVNVNALDDGADICPGNVEFAGNFTNIPHGHGHRVKTSLRLVISDV 546
            IE D    VHFNVLVNV  +DDG+D+CPGN EF G FT +PHGH HR KTSLR+VI++V
Sbjct: 492 GIELDKRFWVHFNVLVNV--VDDGSDVCPGNTEFVGTFTTVPHGHNHRGKTSLRVVITEV 551

Query: 547 IGELGLESNEELAVRIDPQVGGDKVTIGGIRIELQP 577
           + ELG+E +E + VR+DP+VGGDKVTIGG++IELQP
Sbjct: 552 LKELGIEDDESVIVRLDPKVGGDKVTIGGMKIELQP 561

BLAST of MS006101 vs. NCBI nr
Match: KAG7012418.1 (hypothetical protein SDJN02_25170, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 665.6 bits (1716), Expect = 3.8e-187
Identity = 336/576 (58.33%), Postives = 422/576 (73.26%), Query Frame = 0

Query: 7   PYSRTKNQPSSLRNQRQGINYCVVPCRASNNGDQTPINSNPREAKISLNMFDRRDVLFGL 66
           PYS+ KNQ S+L+N+ +GIN+  VPCRAS NGD T             + FDRRDVLFGL
Sbjct: 17  PYSQIKNQTSNLQNKSRGINH--VPCRAS-NGDST-------------SKFDRRDVLFGL 76

Query: 67  GGLYSAATLGGNSS-ASASPQIPDVTICHQAIIEKPRTVDCCPRPPFQADEITHFVLPPA 126
           GGLY AA L    S ASA+P  PDV  C  A IE  + + CCP    +  +I  F+ PP 
Sbjct: 77  GGLYGAAALTNQQSPASAAPLAPDVNNCEPATIEGNQHIPCCPP---KIHDIIDFIHPPH 136

Query: 127 TGKLRVRPAAHLMTPHQVAQFEKAIHIMKNCLDKDDPRHFCQQANVHCAYCNFAYKQEKY 186
           T KLR+RP AH +TP Q   FEKAIHIMK  L  +DPR+F +QA+VHCAYC++AYKQ  +
Sbjct: 137 TTKLRIRPPAHSLTPKQQFNFEKAIHIMKR-LPPNDPRNFYRQADVHCAYCDYAYKQLGF 196

Query: 187 -DVDFEVHWNALFFPFHRAYLYFFERICGHLLGDPDFAMPFWNWDSKPGMYFPQIYNDPN 246
            + + +VHWNALFFPFHRAYLYFFERI GHL+ +P+F +PFWNWD+  GM  P+IY+ PN
Sbjct: 197 PNAEMQVHWNALFFPFHRAYLYFFERILGHLINEPNFVIPFWNWDNTEGMQMPEIYDHPN 256

Query: 247 SPLYDKLRDARHTSGKLLDLNYNPNNEDPSYDVADCNVRWMNKQVLVNGNTAEGFLGKLL 306
           SPLYDKLR+ RH   KLL+LNY+  +++PSYDV D N+RWM+KQ++ + NTAEGF GKLL
Sbjct: 257 SPLYDKLRNQRHRPHKLLNLNYH-GDDNPSYDVVDSNLRWMHKQMITDANTAEGFHGKLL 316

Query: 307 RGGDEA-EGKGMGSIERSPHNNVHDWIGDPPPNTKYGEDMGNFYSAGRDPVFYAHHGNVD 366
               EA E  GMG+IERSPHNN+H+W+GD  PN K   +MG FYSA RDP+FY HH NVD
Sbjct: 317 IASHEANEETGMGTIERSPHNNIHNWVGD--PNAKNHMNMGAFYSAARDPLFYGHHANVD 376

Query: 367 RMWSIWRGLTD-VPRDYTDPDWLDSSFVFYDEHKKAVRITVRQCLESKDLGYVYQDVDIP 426
           RMWSIW+GL +  PRD+ +PDWL+SSFVFY+EHK+AVRI V  CL+++ LGYVYQ VD+P
Sbjct: 377 RMWSIWQGLKNGKPRDFDNPDWLNSSFVFYNEHKQAVRIKVSDCLDTRRLGYVYQYVDLP 436

Query: 427 WLYEKQIPRQRREALSAAGAPQVVSFPLSLDSQINASVERTPWI--GKDMGGADEVLVIE 486
           WL+ KQ PR +++ L    APQV+ FP  L+S +  +V+R   +   K+    DEVLVIE
Sbjct: 437 WLHTKQTPRLKKQ-LKTTQAPQVIKFPQGLNSMVTTTVKRPNKLMNEKEKSEKDEVLVIE 496

Query: 487 DIEFDSDSRVHFNVLVNVNALDDGADICPGNVEFAGNFTNIPHGHGHRVKTSLRLVISDV 546
            IE D    VHFNVLVNV  +DDG+D+CPGN EF G FT +PHGH HR KTSLR+VI++V
Sbjct: 497 GIELDKRFWVHFNVLVNV--VDDGSDVCPGNTEFVGTFTTVPHGHNHRGKTSLRVVITEV 556

Query: 547 IGELGLESNEELAVRIDPQVGGDKVTIGGIRIELQP 577
           + ELG+E +E + VR+DP+VGGDKVTIGG++IE QP
Sbjct: 557 LKELGIEDDESVIVRLDPKVGGDKVTIGGMKIEHQP 566

BLAST of MS006101 vs. NCBI nr
Match: XP_022954613.1 (polyphenol oxidase, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 664.8 bits (1714), Expect = 6.5e-187
Identity = 336/576 (58.33%), Postives = 421/576 (73.09%), Query Frame = 0

Query: 7   PYSRTKNQPSSLRNQRQGINYCVVPCRASNNGDQTPINSNPREAKISLNMFDRRDVLFGL 66
           PYS+ KNQ S+L+N+ +GIN   VPCRAS NGD T             + FDRRDVLFGL
Sbjct: 12  PYSQIKNQTSNLQNKSRGINQ--VPCRAS-NGDST-------------SKFDRRDVLFGL 71

Query: 67  GGLYSAATLGGNSS-ASASPQIPDVTICHQAIIEKPRTVDCCPRPPFQADEITHFVLPPA 126
           GGLY AA L    S ASA+P  PDV  C  A IE    + CCP    +  +I  F+ PP 
Sbjct: 72  GGLYGAAALTNQQSPASAAPLAPDVNNCEAATIEGNEHIPCCPP---KIHDIIDFIHPPH 131

Query: 127 TGKLRVRPAAHLMTPHQVAQFEKAIHIMKNCLDKDDPRHFCQQANVHCAYCNFAYKQEKY 186
           T KLR+RP AH +TP Q   FEKAIHIMK  L  +DPR+F +QA+VHCAYC++AYKQ  +
Sbjct: 132 TTKLRIRPPAHSLTPKQQFNFEKAIHIMKR-LPPNDPRNFYRQADVHCAYCDYAYKQLGF 191

Query: 187 -DVDFEVHWNALFFPFHRAYLYFFERICGHLLGDPDFAMPFWNWDSKPGMYFPQIYNDPN 246
            + + +VHWNALFFPFHRAYLYFFERI GHL+ +P+F +PFWNWD+  GM  P+IY+ PN
Sbjct: 192 PNAEMQVHWNALFFPFHRAYLYFFERILGHLINEPNFVIPFWNWDNTEGMQMPEIYDHPN 251

Query: 247 SPLYDKLRDARHTSGKLLDLNYNPNNEDPSYDVADCNVRWMNKQVLVNGNTAEGFLGKLL 306
           SPLYDKLR+ RH   KLL+LNY+  +++PSYDV D N+RWM+KQ++ + NTAEGF GKLL
Sbjct: 252 SPLYDKLRNQRHRPHKLLNLNYH-GDDNPSYDVVDSNLRWMHKQMITDANTAEGFHGKLL 311

Query: 307 RGGDEA-EGKGMGSIERSPHNNVHDWIGDPPPNTKYGEDMGNFYSAGRDPVFYAHHGNVD 366
               EA E  GMG+IE+SPHNN+H+W+GD  PN K   +MG FYSA RDP+FY HH NVD
Sbjct: 312 IASHEANEETGMGTIEKSPHNNIHNWVGD--PNAKNHMNMGAFYSAARDPLFYGHHANVD 371

Query: 367 RMWSIWRGLTD-VPRDYTDPDWLDSSFVFYDEHKKAVRITVRQCLESKDLGYVYQDVDIP 426
           RMWSIW+GL +   RD+ +PDWL+SSFVFY+EHK+AVRI VR CL+++ LGYVYQ VD+P
Sbjct: 372 RMWSIWQGLKNGKARDFDNPDWLNSSFVFYNEHKQAVRIKVRDCLDTRRLGYVYQYVDLP 431

Query: 427 WLYEKQIPRQRREALSAAGAPQVVSFPLSLDSQINASVERTPWI--GKDMGGADEVLVIE 486
           WL+ KQ PR +++ L    APQV+ FP  L+S +  +V+R   +   K+    DEVLVIE
Sbjct: 432 WLHTKQTPRLKKQ-LKTTQAPQVIKFPQGLNSMVTTTVKRPNKLMNEKEKSEKDEVLVIE 491

Query: 487 DIEFDSDSRVHFNVLVNVNALDDGADICPGNVEFAGNFTNIPHGHGHRVKTSLRLVISDV 546
            IE D    VHFNVLVNV  +DDG+D+CPGN EF G FT +PHGH HR KTSLR+VI++V
Sbjct: 492 GIELDKRFWVHFNVLVNV--VDDGSDVCPGNTEFVGTFTTVPHGHNHRGKTSLRVVITEV 551

Query: 547 IGELGLESNEELAVRIDPQVGGDKVTIGGIRIELQP 577
           + ELG+E +E + VR+DP+VGGDKVTIGG++IELQP
Sbjct: 552 LKELGIEDDESVIVRLDPKVGGDKVTIGGMKIELQP 561

BLAST of MS006101 vs. NCBI nr
Match: XP_022994369.1 (polyphenol oxidase, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 663.3 bits (1710), Expect = 1.9e-186
Identity = 335/576 (58.16%), Postives = 419/576 (72.74%), Query Frame = 0

Query: 7   PYSRTKNQPSSLRNQRQGINYCVVPCRASNNGDQTPINSNPREAKISLNMFDRRDVLFGL 66
           PYS+ KNQ S+ +N+ +GIN+  VPCRAS NGD T             + FDRRDVLFGL
Sbjct: 12  PYSQIKNQTSNRQNKSRGINH--VPCRAS-NGDST-------------SKFDRRDVLFGL 71

Query: 67  GGLYSAATLGGNSS-ASASPQIPDVTICHQAIIEKPRTVDCCPRPPFQADEITHFVLPPA 126
           GGLY AA L    S ASA P  PDV  C  A I     + CCP    +  +I  F+ PP 
Sbjct: 72  GGLYGAAALTNQQSPASAKPLAPDVNNCEPATIVGNEHIPCCPP---KIHDIIDFIHPPH 131

Query: 127 TGKLRVRPAAHLMTPHQVAQFEKAIHIMKNCLDKDDPRHFCQQANVHCAYCNFAYKQEKY 186
           T KLR+R  AH +TP Q   FEKAIHIMK  L  +DPR+F +QA+VHCAYC++AYKQ  +
Sbjct: 132 TTKLRIRQPAHSLTPKQQFNFEKAIHIMKR-LSPNDPRNFYRQADVHCAYCDYAYKQLGF 191

Query: 187 -DVDFEVHWNALFFPFHRAYLYFFERICGHLLGDPDFAMPFWNWDSKPGMYFPQIYNDPN 246
            + + +VHWNALFFPFHRAYLYFFERI GHL+ +PDFA+PFWNWD+K GM  P+IY+ PN
Sbjct: 192 PNAEMQVHWNALFFPFHRAYLYFFERILGHLINEPDFAIPFWNWDNKKGMQMPEIYDHPN 251

Query: 247 SPLYDKLRDARHTSGKLLDLNYNPNNEDPSYDVADCNVRWMNKQVLVNGNTAEGFLGKLL 306
           SPLYDKLR+ RH   KLL+LNY+  +++P YDV DCN+RWM+KQ++ + +TAEGF GKLL
Sbjct: 252 SPLYDKLRNQRHRPHKLLNLNYH-GDDNPPYDVVDCNLRWMHKQMITDADTAEGFHGKLL 311

Query: 307 RGGDEA-EGKGMGSIERSPHNNVHDWIGDPPPNTKYGEDMGNFYSAGRDPVFYAHHGNVD 366
               EA E  GMG+IERSPHNN+H+W+GD  PN K   +MG FYSA RDP+FY HH NVD
Sbjct: 312 VASQEANEETGMGTIERSPHNNIHNWVGD--PNAKNNMNMGVFYSAARDPLFYGHHANVD 371

Query: 367 RMWSIWRGLTD-VPRDYTDPDWLDSSFVFYDEHKKAVRITVRQCLESKDLGYVYQDVDIP 426
           RMWSIW+GL +  PRD+ +PDWL+SSFVFY+EHK+AVRI V  CL+++ LGYVYQ VD+P
Sbjct: 372 RMWSIWQGLKNGKPRDFNNPDWLNSSFVFYNEHKQAVRIKVSDCLDTRRLGYVYQYVDLP 431

Query: 427 WLYEKQIPRQRREALSAAGAPQVVSFPLSLDSQINASVERTPWI--GKDMGGADEVLVIE 486
           WL+ KQ PR +++ L    APQV+ FP  L+S +  +V+R   +   K+    DEVLVIE
Sbjct: 432 WLHTKQTPRLKKQ-LKTTQAPQVIKFPQGLNSMVTTTVKRPKKLRDEKEKSEKDEVLVIE 491

Query: 487 DIEFDSDSRVHFNVLVNVNALDDGADICPGNVEFAGNFTNIPHGHGHRVKTSLRLVISDV 546
            IE D    VHFNVLVNV  +DDG+D+CPGN EF G FT + HGH HR KTSLR+VI++V
Sbjct: 492 GIELDKRYWVHFNVLVNV--VDDGSDVCPGNTEFVGTFTTVLHGHNHRGKTSLRVVITEV 551

Query: 547 IGELGLESNEELAVRIDPQVGGDKVTIGGIRIELQP 577
           + ELG+E +E + VR+DP+VGGDKVTIGG++IELQP
Sbjct: 552 LKELGIEDDESVIVRLDPKVGGDKVTIGGMKIELQP 561

BLAST of MS006101 vs. NCBI nr
Match: XP_023542623.1 (polyphenol oxidase, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 661.0 bits (1704), Expect = 9.4e-186
Identity = 333/571 (58.32%), Postives = 420/571 (73.56%), Query Frame = 0

Query: 12  KNQPSSLRNQRQGINYCVVPCRASNNGDQTPINSNPREAKISLNMFDRRDVLFGLGGLYS 71
           KNQ S L+N+ +GI++  VPCRAS NG+ T             + FDRRDVLFGLGGLY 
Sbjct: 14  KNQTSKLQNKSRGISH--VPCRAS-NGEST-------------SKFDRRDVLFGLGGLYG 73

Query: 72  AATLGGNSS-ASASPQIPDVTICHQAIIEKPRTVDCCPRPPFQADEITHFVLPPATGKLR 131
           AA L    S ASA+P  PDV  C  A IE  + + CCP    +   I  F+ PP T KLR
Sbjct: 74  AAALTNQQSPASAAPLAPDVNNCEPATIEGNQHIPCCPP---KIHHIIDFIHPPHTTKLR 133

Query: 132 VRPAAHLMTPHQVAQFEKAIHIMKNCLDKDDPRHFCQQANVHCAYCNFAYKQEKY-DVDF 191
           +RP AH +TP Q   FEKAIHIMK  L  +DPR+F +QA+VHCAYC++AYKQ  + + + 
Sbjct: 134 IRPPAHSLTPKQQFNFEKAIHIMKR-LPPNDPRNFYRQADVHCAYCDYAYKQLGFPNAEM 193

Query: 192 EVHWNALFFPFHRAYLYFFERICGHLLGDPDFAMPFWNWDSKPGMYFPQIYNDPNSPLYD 251
           +VHWNALFFPFHRAYLYFFERI GHL+ +P+F +PFWNWD+  GM  P+IY++PNSPLYD
Sbjct: 194 QVHWNALFFPFHRAYLYFFERILGHLINEPNFVIPFWNWDNNEGMQMPKIYDNPNSPLYD 253

Query: 252 KLRDARHTSGKLLDLNYNPNNEDPSYDVADCNVRWMNKQVLVNGNTAEGFLGKLLRGGDE 311
           KLR+ RH   KLL+LNY+  +++PSYDV DCN+RWM+KQ++ + +TAEGF GKLL    E
Sbjct: 254 KLRNPRHRPHKLLNLNYH-GDDNPSYDVVDCNLRWMHKQMITDADTAEGFHGKLLIASHE 313

Query: 312 A-EGKGMGSIERSPHNNVHDWIGDPPPNTKYGEDMGNFYSAGRDPVFYAHHGNVDRMWSI 371
           A E  GMG+IERSPHNN+H+W+GD  PN K   +MG FYSA RDP+FY HH NVDRMWSI
Sbjct: 314 ANEETGMGTIERSPHNNIHNWVGD--PNAKNHMNMGAFYSAARDPLFYGHHANVDRMWSI 373

Query: 372 WRGLTD-VPRDYTDPDWLDSSFVFYDEHKKAVRITVRQCLESKDLGYVYQDVDIPWLYEK 431
           W+GL +  PRD+ +PDWL+SSFVFY+EHK+AVRI V  CL+++ LGYVYQ VD+PWL+ K
Sbjct: 374 WQGLKNGKPRDFDNPDWLNSSFVFYNEHKQAVRIKVSDCLDTRRLGYVYQYVDLPWLHTK 433

Query: 432 QIPRQRREALSAAGAPQVVSFPLSLDSQINASVERTPWI--GKDMGGADEVLVIEDIEFD 491
           Q PR +++ L A  APQV+ FP  L+S +  +V+R   +   K+    DEVLVIE IE D
Sbjct: 434 QTPRLKKQ-LKATQAPQVIKFPQGLNSMVTTTVKRPNKLRDEKEKSEKDEVLVIEGIELD 493

Query: 492 SDSRVHFNVLVNVNALDDGADICPGNVEFAGNFTNIPHGHGHRVKTSLRLVISDVIGELG 551
               VHFNVLVNV  +DDG+D+CPGN EF G FT +PHGH HR KTSLR+VI++V+ ELG
Sbjct: 494 KRYWVHFNVLVNV--VDDGSDVCPGNTEFVGTFTTVPHGHNHRGKTSLRVVITEVLKELG 553

Query: 552 LESNEELAVRIDPQVGGDKVTIGGIRIELQP 577
           +E +E + VR+DP+VGGDKVTIGG++IELQP
Sbjct: 554 IEDDESVIVRLDPKVGGDKVTIGGMKIELQP 558

BLAST of MS006101 vs. ExPASy Swiss-Prot
Match: P43309 (Polyphenol oxidase, chloroplastic OS=Malus domestica OX=3750 PE=1 SV=1)

HSP 1 Score: 455.3 bits (1170), Expect = 1.0e-126
Identity = 271/594 (45.62%), Postives = 357/594 (60.10%), Query Frame = 0

Query: 6   SPYSRTKNQPSSLRNQRQGINYCVVPCRASNNGDQTPINSNPREAKISLNMFDRRDVLFG 65
           SP+S+  +Q S L   ++      V C+A+NN       S            DRR+VL G
Sbjct: 23  SPFSQNNSQVSLLTKPKRSFAR-KVSCKATNNDQNDQAQSK----------LDRRNVLLG 82

Query: 66  LGGLYSAATLGGNSSASASP-QIPDVTICHQAIIEKPRT-VDCCPRPPFQADEITHFVLP 125
           LGGLY  A +G +  A A P   PDV+ C  A + +     +CCP P   + +I  F L 
Sbjct: 83  LGGLYGVAGMGTDPFAFAKPIAPPDVSKCGPADLPQGAVPTNCCPPP---STKIIDFKL- 142

Query: 126 PATGKLRVRPAAHLMTPHQVAQFEKAIHIMKNCLDKDDPRHFCQQANVHCAYCNFAYKQE 185
           PA  KLR+RP AH +      ++ KA+ +MK  L  DDPR F QQA VHCAYC+ AY Q 
Sbjct: 143 PAPAKLRIRPPAHAVDQAYRDKYYKAMELMK-ALPDDDPRSFKQQAAVHCAYCDGAYDQV 202

Query: 186 KY-DVDFEVHWNALFFPFHRAYLYFFERICGHLLGDPDFAMPFWNWDSKPGMYFPQIYND 245
            + +++ ++H + LFFPFHR YLYFFE+I G L+ DP FA+PFWNWDS  GM  P IY D
Sbjct: 203 GFPELELQIHNSWLFFPFHRYYLYFFEKILGKLINDPTFALPFWNWDSPAGMPLPAIYAD 262

Query: 246 PNSPLYDKLRDARHTSGKLLDLNYNPNNEDPSYDVA-DCNVRWMNKQVLVNGNTAEGFLG 305
           P SPLYDKLR A H    L+DL+YN   ++ S +   + N++ M +Q++ N   A+ F G
Sbjct: 263 PKSPLYDKLRSANHQPPTLVDLDYNGTEDNVSKETTINANLKIMYRQMVSNSKNAKLFFG 322

Query: 306 KLLRGGDEAEGKGMGSIERSPHNNVHDWIGDPPPNTKYG-EDMGNFYSAGRDPVFYAHHG 365
              R GDE +  G GSIE +PH  VH W GD   NT+   EDMGNFYSAGRDP+F+AHH 
Sbjct: 323 NPYRAGDEPD-PGGGSIEGTPHAPVHLWTGD---NTQPNFEDMGNFYSAGRDPIFFAHHS 382

Query: 366 NVDRMWSIWRGLTDVPRDYTDPDWLDSSFVFYDEHKKAVRITVRQCLESKDLGYVYQDVD 425
           NVDRMWSIW+ L     D TD DWLDS F+FY+E+ + VR+ VR CLE+K+LGYVYQDVD
Sbjct: 383 NVDRMWSIWKTLGGKRTDLTDSDWLDSGFLFYNENAELVRVKVRDCLETKNLGYVYQDVD 442

Query: 426 IPWLYEKQIPRQRREALS------------AAGAPQVVS---FPLSLDSQINASVERTPW 485
           IPWL  K  PR+ + ALS             A + +VV+   FP+SL S+I+  V+R   
Sbjct: 443 IPWLSSKPTPRRAKVALSKVAKKLGVAHAAVASSSKVVAGTEFPISLGSKISTVVKRPKQ 502

Query: 486 ---IGKDMGGADEVLVIEDIEFDSDSRVHFNVLVN-VNALDDGADICPGNVEFAGNFTNI 545
                K     +E+LVIE IEFD D  V F+V VN V+ L  G    P   EFAG+F ++
Sbjct: 503 KKRSKKAKEDEEEILVIEGIEFDRDVAVKFDVYVNDVDDLPSG----PDKTEFAGSFVSV 562

Query: 546 PHGHGHRVK--TSLRLVISDVIGELGLESNEELAVRIDPQVGGDKVTIGGIRIE 574
           PH H H+ K  T LRL ++D++ E+  E ++ + V + P+ G   V IGGI+IE
Sbjct: 563 PHSHKHKKKMNTILRLGLTDLLEEIEAEDDDSVVVTLVPKFGA--VKIGGIKIE 590

BLAST of MS006101 vs. ExPASy Swiss-Prot
Match: O81103 (Polyphenol oxidase latent form, chloroplastic OS=Prunus armeniaca OX=36596 GN=PPO PE=1 SV=1)

HSP 1 Score: 422.9 bits (1086), Expect = 5.7e-117
Identity = 241/558 (43.19%), Postives = 323/558 (57.89%), Query Frame = 0

Query: 30  VPCRASNNGDQTPINSNPREAKISLNMFDRRDVLFGLGGLYSAATLGGNSSASASP-QIP 89
           + C+A+NN +    N    E+   L   DRR++L GLGGLY A TL     A A P   P
Sbjct: 48  ISCKATNNNNSNNQNEQQEESSRLLGKLDRRNILIGLGGLYGATTLDRKPFAFADPIAPP 107

Query: 90  DVTICHQAIIEK--PRTVDCCPRPPFQADEITHFVLPPATGKLRVRPAAHLMTPHQVAQF 149
           D+T C  A I      TV CCP       +I  F  P  +  LR  PAAH +T   +A+F
Sbjct: 108 DLTTCKPAEITPGGSETVPCCPP---VTTKIKTF-KPDLSIPLRTSPAAHQVTDEYLAKF 167

Query: 150 EKAIHIMKNCLDKDDPRHFCQQANVHCAYCNFAYKQEKY-DVDFEVHWNALFFPFHRAYL 209
           +KA   M+  L  DDPR   QQA VHCAYCN AY Q  + D D +VH++ LFFPFHR YL
Sbjct: 168 KKAQAAMR-ALPDDDPRSMVQQAKVHCAYCNGAYPQVGFTDNDIQVHFSWLFFPFHRMYL 227

Query: 210 YFFERICGHLLGDPDFAMPFWNWDSKPGMYFPQIYNDPNSPLYDKLRDARHTSGKLLDLN 269
           YF+ERI G L+ DP FA+P+WNWDS  G   P IY D +SPLYD+ R+A H    L+DL+
Sbjct: 228 YFYERILGKLIDDPTFALPYWNWDSPVGFPIPDIYTDTSSPLYDQYRNADHQPPVLVDLS 287

Query: 270 YNPNNEDPSYDV-ADCNVRWMNKQVLVNGNTAEGFLGKLLRGGDEAEGKGMGSIERSPHN 329
           Y   ++D       D N+  M +Q++    T + F G   R G+   GK  G+IE  PHN
Sbjct: 288 YGGKDDDVDEQTRIDENLAIMYRQMVSGAKTPDLFFGHAYRAGNLNTGKYPGTIENMPHN 347

Query: 330 NVHDWIGDPPPNTKYGEDMGNFYSAGRDPVFYAHHGNVDRMWSIWRGLTDVPRDYTDPDW 389
           N+H W+GD  P+  + EDMGNFYSAGRDP+FYAHH NVDRMW+IW+ L    +D TD DW
Sbjct: 348 NIHIWVGD--PSQTHQEDMGNFYSAGRDPLFYAHHANVDRMWNIWKTLGGKRKDITDTDW 407

Query: 390 LDSSFVFYDEHKKAVRITVRQCLE-SKDLGYVYQDVDIPWLYEKQIPRQRREALSA-AGA 449
           LD+ F+FYDE+ + VR+ VR  LE  K L Y Y+ V +PWL+ K   R+ +    A   A
Sbjct: 408 LDAEFLFYDENAELVRVKVRDSLEPEKQLRYNYEPVSLPWLFTKPTARKTKNKTKAKVAA 467

Query: 450 PQVVS-FPLSLDSQINASVER---TPWIGKDMGGADEVLVIEDIEFDSDSRVHFNVLVNV 509
            Q+ S FP +L       V R        K+    +E+L+I+DIEF+    V F+V +N 
Sbjct: 468 TQLTSKFPATLVEVTTVEVARPKPRKRSKKEKVDEEELLIIKDIEFEGTEAVKFDVFIN- 527

Query: 510 NALDDGADIC-PGNVEFAGNFTNIPHGH--GHRVKTSLRLVISDVIGELGLESNEELAVR 569
              DD   +      EFAG+F ++P G     + KT+L+L I+D++ +LG E +  + V 
Sbjct: 528 ---DDAESLSRRDKSEFAGSFVHVPQGKTTKAKTKTNLKLGITDLLEDLGAEDDSSVLVT 587

Query: 570 IDPQVGGDKVTIGGIRIE 574
           + P+V    +TIGG +IE
Sbjct: 588 LVPRVSNSPITIGGFKIE 594

BLAST of MS006101 vs. ExPASy Swiss-Prot
Match: Q9MB14 (Polyphenol oxidase II, chloroplastic OS=Ipomoea batatas OX=4120 GN=co-2 PE=2 SV=2)

HSP 1 Score: 420.6 bits (1080), Expect = 2.8e-116
Identity = 244/586 (41.64%), Postives = 340/586 (58.02%), Query Frame = 0

Query: 2   LSLSSPYSRTKNQPSSLR-NQRQGINYCVVPCRASNNGDQTPINSNPREAKISLNMFDRR 61
           LS S+  S    +PS +     +  ++  V C A  NGD  P               DRR
Sbjct: 20  LSSSATISSPLPKPSQIHIATAKRTHHFKVSCNAP-NGDSQP-------------KLDRR 79

Query: 62  DVLFGLGGLYSAATLGGNSSASASP-QIPDVTICHQAIIEKPRTV---DCCPRPPFQADE 121
           DVL GLGGL  AA+L  N  A A P   P+++ C     + P      +CC  PP   + 
Sbjct: 80  DVLLGLGGLAGAASLINNPLAFAEPIHAPEISKCVVPPKDLPPDAIVDNCC--PPLATNV 139

Query: 122 ITHFVLPPATGKLRVRPAAHLMTPHQVAQFEKAIHIMKNCLDKDDPRHFCQQANVHCAYC 181
           I + V   +   +++RPA H M    +A+FEKAI +MK  L  DDPR+F QQA VHCAYC
Sbjct: 140 IPYKVPKTSPSAMKIRPAIHRMDKEYIAKFEKAIRLMKE-LPADDPRNFYQQALVHCAYC 199

Query: 182 NFAYKQEKY-DVDFEVHWNALFFPFHRAYLYFFERICGHLLGDPDFAMPFWNWDSKPGMY 241
           N  Y Q  Y D + +VH + LFFPFHR YLYF+ERI G L+GDP F +PFWNWD+  GM 
Sbjct: 200 NGGYVQTDYPDKEIQVHNSWLFFPFHRWYLYFYERILGKLIGDPTFGLPFWNWDTPAGML 259

Query: 242 FPQIYNDPNSPLYDKLRDARHTSGKLLDLNYNPNNED-PSYDVADCNVRWMNKQVLVNGN 301
            PQ + + NSPLYD+ R+  H    ++DL Y   + D    +    N+  M K ++ N  
Sbjct: 260 IPQYFRNQNSPLYDENRNQSHLP-LVMDLGYAGTDTDVTDQERISNNLALMYKSMVTNAG 319

Query: 302 TAEGFLGKLLRGGDEAEGKGMGSIERSPHNNVHDWIGDPPPNTKYGEDMGNFYSAGRDPV 361
           TAE FLGK  + GD+   KG GSIE  PH  VH W+GD  P T+ GEDMGNFYSAGRD +
Sbjct: 320 TAELFLGKPYKAGDDPVNKGGGSIENIPHTPVHRWVGDVKPRTQNGEDMGNFYSAGRDIL 379

Query: 362 FYAHHGNVDRMWSIWR--GLTDVPRDYTDPDWLDSSFVFYDEHKKAVRITVRQCLESKDL 421
           FY HH NVDRMW+IW+  G     RD+TD DWLD++F+FYDE+K+AVR+ V   L+++ L
Sbjct: 380 FYCHHSNVDRMWTIWQQLGGKGRRRDFTDSDWLDATFIFYDENKQAVRVRVGDALDNQKL 439

Query: 422 GYVYQDVDIPWLYEKQIPRQRREALSA-AGAPQVVS-FPLSLDSQINASVER--TPWIGK 481
           GY Y+  ++PWL  K +P +++  L+A + AP V   FPL+LD  +   V R       +
Sbjct: 440 GYKYEFTNLPWLNSKPLPTKKKTGLAARSKAPFVTDVFPLTLDKVVQVKVPRPKKSRSKE 499

Query: 482 DMGGADEVLVIEDIEFDSDSRVHFNVLVNVNALDDGADICPGNVEFAGNFTNIPHGH--G 541
           +    +E+L I+ IE   D    F+V +N    +D  +      E+AG+F ++PH H   
Sbjct: 500 EKEAEEEILEIQGIEVAIDQYAKFDVYLND---EDEPEAGKEKAEYAGSFAHLPHKHTGS 559

Query: 542 HRVKTSLRLVISDVIGELGLESNEELAVRIDPQVGGDKVTIGGIRI 573
            +++TSL L +++ + +LG E ++ + V + P+VGG  VT+  I+I
Sbjct: 560 KKIRTSLSLGLNEPLEDLGAEDDDAVLVTLAPKVGGGVVTVENIKI 584

BLAST of MS006101 vs. ExPASy Swiss-Prot
Match: Q06215 (Polyphenol oxidase A1, chloroplastic OS=Vicia faba OX=3906 PE=1 SV=1)

HSP 1 Score: 418.7 bits (1075), Expect = 1.1e-115
Identity = 253/601 (42.10%), Postives = 351/601 (58.40%), Query Frame = 0

Query: 7   PYSRTKNQPSSLRNQRQGINYCVVPCRASNNGDQTPINSNPREAKISLNMF-DRRDVLFG 66
           P+ + ++Q S LR  ++      V C ++NN       +NP+E +   N+   RR+VL G
Sbjct: 28  PFLQKQHQSSKLRKPKR-----QVTCSSNNN------QNNPKEEQELSNIVGHRRNVLIG 87

Query: 67  LGGLYSAATLGGNSSASASP-QIPDVTIC----HQAIIEKPRTVDCCPRPPFQADEITHF 126
           LGG+Y   TL  N SA ASP   PD++ C           P  ++CC  PP+ + +IT F
Sbjct: 88  LGGIY--GTLATNPSALASPISPPDLSKCVPPSDLPSGTTPPNINCC--PPY-STKITDF 147

Query: 127 VLPPATGKLRVRPAAHLMTPHQVAQFEKAIHIMKNCLDKDDPRHFCQQANVHCAYCNFAY 186
              P+   LRVR AAHL+    + +++KA  +MK  L  +DPR+F QQAN+HCAYC+ AY
Sbjct: 148 KF-PSNQPLRVRQAAHLVDNEFLEKYKKATELMK-ALPSNDPRNFTQQANIHCAYCDGAY 207

Query: 187 KQEKY-DVDFEVHWNALFFPFHRAYLYFFERICGHLLGDPDFAMPFWNWDSKPGMYFPQI 246
            Q  + D+  +VH + LFFPFHR YLYF+ERI G L+ DP FA+PFWN+D+  GM  P I
Sbjct: 208 SQIGFPDLKLQVHGSWLFFPFHRWYLYFYERILGSLINDPTFALPFWNYDAPDGMQLPTI 267

Query: 247 YNDPNSPLYDKLRDARHTSGKLLDLNYNPNNED-PSYDVADCNVRWMNKQVLVNGNTAEG 306
           Y D  SPLYD+LR+A H    L+DLN+     D    ++   N+  M +QV  NG T+  
Sbjct: 268 YADKASPLYDELRNASHQPPTLIDLNFCDIGSDIDRNELIKTNLSIMYRQVYSNGKTSRL 327

Query: 307 FLGKLLRGGDEAEGKGMGSIERSPHNNVHDWIGDPPPNTKYG-EDMGNFYSAGRDPVFYA 366
           FLG   R GD AE +G GSIE  PH  VH W GD   NT+   EDMG FYSA RDP+FY+
Sbjct: 328 FLGNPYRAGD-AEPQGAGSIENVPHAPVHTWTGD---NTQTNIEDMGIFYSAARDPIFYS 387

Query: 367 HHGNVDRMWSIWRGLTDVPRDYTDPDWLDSSFVFYDEHKKAVRITVRQCLESKDLGYVYQ 426
           HH NVDR+W IW+ L     D+TD DWL+S F+FYDE+K  VR+ V+  L+   LGY YQ
Sbjct: 388 HHSNVDRLWYIWKTLGGKKHDFTDKDWLESGFLFYDENKNLVRVNVKDSLDIDKLGYAYQ 447

Query: 427 DVDIPWLYEKQIPRQRRE--------------------ALSAAGAPQVVSFPLSLDSQIN 486
           DV IPW   K +PR+ +                      +      + V+FPL L+++++
Sbjct: 448 DVPIPWEKAKPVPRRTKVQKLVEVEVNDGNLRKSPTIFLVRQQSPRKYVTFPLVLNNKVS 507

Query: 487 ASVERTPWI--GKDMGGADEVLVIEDIEFDSDSRVHFNVLVNVNALDDGADICPGNVEFA 546
           A V+R   +   K+    +EVLVIE IEF  +  + F+V +N    D+   +  GN EFA
Sbjct: 508 AIVKRPKKLRSKKEKEEEEEVLVIEGIEFYMNIAIKFDVYIN----DEDDKVGAGNTEFA 567

Query: 547 GNFTNIPH-GHGHRVK---TSLRLVISDVIGELGLESNEELAVRIDPQVGGDKVTIGGIR 573
           G+F NIPH  HGH+ K   TSLRL I+D++ +L +E ++ + V + P+ G  +V I  + 
Sbjct: 568 GSFVNIPHSAHGHKNKKIITSLRLGITDLLEDLHVEGDDNIVVTLVPKCGSGQVKINNVE 602

BLAST of MS006101 vs. ExPASy Swiss-Prot
Match: Q6UIL3 ((+)-larreatricin hydroxylase, chloroplastic OS=Larrea tridentata OX=66636 PE=1 SV=1)

HSP 1 Score: 411.4 bits (1056), Expect = 1.7e-113
Identity = 247/594 (41.58%), Postives = 345/594 (58.08%), Query Frame = 0

Query: 4   LSSPYS---RTKNQPSSLRNQRQGINYCVVPCRASNNGDQTPINSNPREAKISLNMFDRR 63
           L++PYS    TK    SLR          V C+ASN+    P   N +E   S+   DRR
Sbjct: 11  LATPYSFPYHTKPSRVSLRR---------VSCKASNDNKDKP---NDQEKTFSI---DRR 70

Query: 64  DVLFGLGGLYSAATL-GGNSSASASPQIPDV--TICH-QAIIEKPRTVDCCPRPPFQADE 123
           ++L GLGGLY A+ +   N S  A+P  P V    CH    + +   V CC  PP   D 
Sbjct: 71  NMLIGLGGLYGASNVFPSNQSTLAAPIQPPVLPDSCHPPEDLAEGVNVLCC--PPDVKDP 130

Query: 124 ITHFVLPPATGKLRVRPAAHLMTPHQVAQFEKAIHIMKNCLDKDDPRHFCQQANVHCAYC 183
           I  F +P    +LR+RPAAHL  P  + +++KA+  MK  L ++DPR F QQAN+HCAYC
Sbjct: 131 I-DFQMPSNPSRLRIRPAAHLADPTYIEKYKKALAAMK-ALPQNDPRSFYQQANIHCAYC 190

Query: 184 NFAYKQEKY-DVDFEVHWNALFFPFHRAYLYFFERICGHLLGDPDFAMPFWNWDSKPGMY 243
           N AY Q  + DV+ +VH + LF PFHR YLYF+ERI G L+ DP FA+PFWNWD+  GM+
Sbjct: 191 NGAYDQVGFPDVNIQVHHSWLFLPFHRWYLYFYERILGSLIDDPTFAIPFWNWDAPKGMH 250

Query: 244 FPQIYNDPNSPLYDKLRDARHTSGKLLDLNYNPNNEDPSYDVADC--NVRWMNKQVLVNG 303
            P ++ DPNSPLYD  R+  H    ++DL+++ + E PS++      N+  M KQV V  
Sbjct: 251 MPHMFIDPNSPLYDAKRNPAHFPDTIVDLDFS-SGEAPSHNPRQIGNNLSIMYKQV-VRA 310

Query: 304 NTAEGFLGKLLRGGDEAEGKGMGSIERSPHNNVHDWIGDPPPNTKYGEDMGNFYSAGRDP 363
             A  F G+ L+ G   +  G GS+E +PH N+H W GDP  +    E+MGNFYSAGRDP
Sbjct: 311 KKARLFHGRPLQAGSFPDESGDGSLEGTPHGNIHLWSGDPRQSN--FENMGNFYSAGRDP 370

Query: 364 VFYAHHGNVDRMWSIWRGLTDVPRDYTDPDWLDSSFVFYDEHKKAVRITVRQCLESKDLG 423
           +FYAHH NVDRMW IW+      +DY   DWL++ F+ +DE+ + VR+ VR  L+ + LG
Sbjct: 371 LFYAHHANVDRMWYIWKNSLG-RKDYKKKDWLNAGFLLFDENAQPVRVYVRDALDERKLG 430

Query: 424 YVYQDVDIPWLYEKQIPRQ------RREALSAAGAPQVVSFPLSLDSQINASVER--TPW 483
           Y YQ+VDIPW+  K  PR+      R  + +      +  FPL+LDS ++  V+R     
Sbjct: 431 YAYQEVDIPWINSKPKPRKANPWPNRLRSKATTTTKLINKFPLTLDSTVSFEVKRPKKSR 490

Query: 484 IGKDMGGADEVLVIEDIEFDSDSRVHFNVLVNVNALDDGADICPGNVEFAGNFTNIPHGH 543
              +    +EVLVIE I+ +    + F+V +N    D  A       EFAG+F N+PH H
Sbjct: 491 SKSEKEDEEEVLVIEKIKHEPQFPLKFDVYINDEDEDPSA---ADQTEFAGSFVNVPHFH 550

Query: 544 GH------RVKTSLRLVISDVIGELGLESNEELAVRIDPQVGGDKVTIGGIRIE 574
            H      R  T+L + IS+V+ EL ++ ++ + V + P+VG  ++TIGG +IE
Sbjct: 551 RHGDKKDKRQTTNLSIGISEVLDELDVDGDDSIVVTLVPRVGSGQITIGGAKIE 577

BLAST of MS006101 vs. ExPASy TrEMBL
Match: A0A6J1GRK5 (polyphenol oxidase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111456826 PE=3 SV=1)

HSP 1 Score: 664.8 bits (1714), Expect = 3.2e-187
Identity = 336/576 (58.33%), Postives = 421/576 (73.09%), Query Frame = 0

Query: 7   PYSRTKNQPSSLRNQRQGINYCVVPCRASNNGDQTPINSNPREAKISLNMFDRRDVLFGL 66
           PYS+ KNQ S+L+N+ +GIN   VPCRAS NGD T             + FDRRDVLFGL
Sbjct: 12  PYSQIKNQTSNLQNKSRGINQ--VPCRAS-NGDST-------------SKFDRRDVLFGL 71

Query: 67  GGLYSAATLGGNSS-ASASPQIPDVTICHQAIIEKPRTVDCCPRPPFQADEITHFVLPPA 126
           GGLY AA L    S ASA+P  PDV  C  A IE    + CCP    +  +I  F+ PP 
Sbjct: 72  GGLYGAAALTNQQSPASAAPLAPDVNNCEAATIEGNEHIPCCPP---KIHDIIDFIHPPH 131

Query: 127 TGKLRVRPAAHLMTPHQVAQFEKAIHIMKNCLDKDDPRHFCQQANVHCAYCNFAYKQEKY 186
           T KLR+RP AH +TP Q   FEKAIHIMK  L  +DPR+F +QA+VHCAYC++AYKQ  +
Sbjct: 132 TTKLRIRPPAHSLTPKQQFNFEKAIHIMKR-LPPNDPRNFYRQADVHCAYCDYAYKQLGF 191

Query: 187 -DVDFEVHWNALFFPFHRAYLYFFERICGHLLGDPDFAMPFWNWDSKPGMYFPQIYNDPN 246
            + + +VHWNALFFPFHRAYLYFFERI GHL+ +P+F +PFWNWD+  GM  P+IY+ PN
Sbjct: 192 PNAEMQVHWNALFFPFHRAYLYFFERILGHLINEPNFVIPFWNWDNTEGMQMPEIYDHPN 251

Query: 247 SPLYDKLRDARHTSGKLLDLNYNPNNEDPSYDVADCNVRWMNKQVLVNGNTAEGFLGKLL 306
           SPLYDKLR+ RH   KLL+LNY+  +++PSYDV D N+RWM+KQ++ + NTAEGF GKLL
Sbjct: 252 SPLYDKLRNQRHRPHKLLNLNYH-GDDNPSYDVVDSNLRWMHKQMITDANTAEGFHGKLL 311

Query: 307 RGGDEA-EGKGMGSIERSPHNNVHDWIGDPPPNTKYGEDMGNFYSAGRDPVFYAHHGNVD 366
               EA E  GMG+IE+SPHNN+H+W+GD  PN K   +MG FYSA RDP+FY HH NVD
Sbjct: 312 IASHEANEETGMGTIEKSPHNNIHNWVGD--PNAKNHMNMGAFYSAARDPLFYGHHANVD 371

Query: 367 RMWSIWRGLTD-VPRDYTDPDWLDSSFVFYDEHKKAVRITVRQCLESKDLGYVYQDVDIP 426
           RMWSIW+GL +   RD+ +PDWL+SSFVFY+EHK+AVRI VR CL+++ LGYVYQ VD+P
Sbjct: 372 RMWSIWQGLKNGKARDFDNPDWLNSSFVFYNEHKQAVRIKVRDCLDTRRLGYVYQYVDLP 431

Query: 427 WLYEKQIPRQRREALSAAGAPQVVSFPLSLDSQINASVERTPWI--GKDMGGADEVLVIE 486
           WL+ KQ PR +++ L    APQV+ FP  L+S +  +V+R   +   K+    DEVLVIE
Sbjct: 432 WLHTKQTPRLKKQ-LKTTQAPQVIKFPQGLNSMVTTTVKRPNKLMNEKEKSEKDEVLVIE 491

Query: 487 DIEFDSDSRVHFNVLVNVNALDDGADICPGNVEFAGNFTNIPHGHGHRVKTSLRLVISDV 546
            IE D    VHFNVLVNV  +DDG+D+CPGN EF G FT +PHGH HR KTSLR+VI++V
Sbjct: 492 GIELDKRFWVHFNVLVNV--VDDGSDVCPGNTEFVGTFTTVPHGHNHRGKTSLRVVITEV 551

Query: 547 IGELGLESNEELAVRIDPQVGGDKVTIGGIRIELQP 577
           + ELG+E +E + VR+DP+VGGDKVTIGG++IELQP
Sbjct: 552 LKELGIEDDESVIVRLDPKVGGDKVTIGGMKIELQP 561

BLAST of MS006101 vs. ExPASy TrEMBL
Match: A0A6J1K2N9 (polyphenol oxidase, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111490108 PE=3 SV=1)

HSP 1 Score: 663.3 bits (1710), Expect = 9.2e-187
Identity = 335/576 (58.16%), Postives = 419/576 (72.74%), Query Frame = 0

Query: 7   PYSRTKNQPSSLRNQRQGINYCVVPCRASNNGDQTPINSNPREAKISLNMFDRRDVLFGL 66
           PYS+ KNQ S+ +N+ +GIN+  VPCRAS NGD T             + FDRRDVLFGL
Sbjct: 12  PYSQIKNQTSNRQNKSRGINH--VPCRAS-NGDST-------------SKFDRRDVLFGL 71

Query: 67  GGLYSAATLGGNSS-ASASPQIPDVTICHQAIIEKPRTVDCCPRPPFQADEITHFVLPPA 126
           GGLY AA L    S ASA P  PDV  C  A I     + CCP    +  +I  F+ PP 
Sbjct: 72  GGLYGAAALTNQQSPASAKPLAPDVNNCEPATIVGNEHIPCCPP---KIHDIIDFIHPPH 131

Query: 127 TGKLRVRPAAHLMTPHQVAQFEKAIHIMKNCLDKDDPRHFCQQANVHCAYCNFAYKQEKY 186
           T KLR+R  AH +TP Q   FEKAIHIMK  L  +DPR+F +QA+VHCAYC++AYKQ  +
Sbjct: 132 TTKLRIRQPAHSLTPKQQFNFEKAIHIMKR-LSPNDPRNFYRQADVHCAYCDYAYKQLGF 191

Query: 187 -DVDFEVHWNALFFPFHRAYLYFFERICGHLLGDPDFAMPFWNWDSKPGMYFPQIYNDPN 246
            + + +VHWNALFFPFHRAYLYFFERI GHL+ +PDFA+PFWNWD+K GM  P+IY+ PN
Sbjct: 192 PNAEMQVHWNALFFPFHRAYLYFFERILGHLINEPDFAIPFWNWDNKKGMQMPEIYDHPN 251

Query: 247 SPLYDKLRDARHTSGKLLDLNYNPNNEDPSYDVADCNVRWMNKQVLVNGNTAEGFLGKLL 306
           SPLYDKLR+ RH   KLL+LNY+  +++P YDV DCN+RWM+KQ++ + +TAEGF GKLL
Sbjct: 252 SPLYDKLRNQRHRPHKLLNLNYH-GDDNPPYDVVDCNLRWMHKQMITDADTAEGFHGKLL 311

Query: 307 RGGDEA-EGKGMGSIERSPHNNVHDWIGDPPPNTKYGEDMGNFYSAGRDPVFYAHHGNVD 366
               EA E  GMG+IERSPHNN+H+W+GD  PN K   +MG FYSA RDP+FY HH NVD
Sbjct: 312 VASQEANEETGMGTIERSPHNNIHNWVGD--PNAKNNMNMGVFYSAARDPLFYGHHANVD 371

Query: 367 RMWSIWRGLTD-VPRDYTDPDWLDSSFVFYDEHKKAVRITVRQCLESKDLGYVYQDVDIP 426
           RMWSIW+GL +  PRD+ +PDWL+SSFVFY+EHK+AVRI V  CL+++ LGYVYQ VD+P
Sbjct: 372 RMWSIWQGLKNGKPRDFNNPDWLNSSFVFYNEHKQAVRIKVSDCLDTRRLGYVYQYVDLP 431

Query: 427 WLYEKQIPRQRREALSAAGAPQVVSFPLSLDSQINASVERTPWI--GKDMGGADEVLVIE 486
           WL+ KQ PR +++ L    APQV+ FP  L+S +  +V+R   +   K+    DEVLVIE
Sbjct: 432 WLHTKQTPRLKKQ-LKTTQAPQVIKFPQGLNSMVTTTVKRPKKLRDEKEKSEKDEVLVIE 491

Query: 487 DIEFDSDSRVHFNVLVNVNALDDGADICPGNVEFAGNFTNIPHGHGHRVKTSLRLVISDV 546
            IE D    VHFNVLVNV  +DDG+D+CPGN EF G FT + HGH HR KTSLR+VI++V
Sbjct: 492 GIELDKRYWVHFNVLVNV--VDDGSDVCPGNTEFVGTFTTVLHGHNHRGKTSLRVVITEV 551

Query: 547 IGELGLESNEELAVRIDPQVGGDKVTIGGIRIELQP 577
           + ELG+E +E + VR+DP+VGGDKVTIGG++IELQP
Sbjct: 552 LKELGIEDDESVIVRLDPKVGGDKVTIGGMKIELQP 561

BLAST of MS006101 vs. ExPASy TrEMBL
Match: A0A172DC01 (Polyphenol oxidase OS=Luffa aegyptiaca OX=3670 GN=PPO2 PE=2 SV=1)

HSP 1 Score: 647.5 bits (1669), Expect = 5.2e-182
Identity = 344/582 (59.11%), Postives = 417/582 (71.65%), Query Frame = 0

Query: 1   LLSLSSPYSRTKNQPSSLRNQRQGINYCVVPCRASNNGDQTPINSNPREAKISLNMFDRR 60
           LLSL  P  R+KNQ SS  N+R G++Y  +PCRAS NG QTP+NSNP+E   S++ FDRR
Sbjct: 7   LLSL-FPCIRSKNQSSSRGNRRLGLSY--IPCRAS-NGVQTPMNSNPQEGD-SMSKFDRR 66

Query: 61  DVLFGLGGLYSAATLGGNSSASASPQIPDVTICHQAIIEKPRTVDCCPRPPFQADEITHF 120
           DVLFGLGGLYSAA LG + SA A P  PDV  C    +E    VDCCP       +IT F
Sbjct: 67  DVLFGLGGLYSAAALGRDRSALADPISPDVIHCEPITLEADEKVDCCPPSIL---DITDF 126

Query: 121 VLPPATGKLRVRPAAHLMTPHQVAQFEKAIHIMKNCLDKDDPRHFCQQANVHCAYCNFAY 180
           V PPA   LRVRPAAHL+T  Q+A+ E+A+  MK  LD DDPR+F QQA VHCAYCN AY
Sbjct: 127 V-PPAVENLRVRPAAHLITQEQLAKLEEAVTKMK-ALDPDDPRNFYQQARVHCAYCNDAY 186

Query: 181 KQEKY-DVDFEVHWNALFFPFHRAYLYFFERICGHLLGDPDFAMPFWNWDSKPGMYFPQI 240
           KQ+ + ++DF++HWN LFFPFHR YLYF ERI G L+ DPDFA+PFWNWD+ PGM  P I
Sbjct: 187 KQDGFPELDFDIHWNWLFFPFHRCYLYFHERILGSLINDPDFAIPFWNWDAIPGMQMPAI 246

Query: 241 Y--NDPNSPLYDKLRDARHTSGKLLDLNYNPNNEDPSYDVADCNVRWMNKQVLVNGNTAE 300
           Y  +D NSPLYD+LRD  H   KLLDLNY+   EDPSYDV  CN R M +Q+++N  TAE
Sbjct: 247 YINDDKNSPLYDELRDPNHKPPKLLDLNYDGATEDPSYDVLFCNPRLMYRQMVINSKTAE 306

Query: 301 GFLGKLLRGGDEAEGKGMGSIERSPHNNVHDWIGDPPPNTKYGEDMGNFYSAGRDPVFYA 360
           GF GK LR G EA   GMGSIE S HNNVH W GD  P  KY EDMG+FY+A RDP+FYA
Sbjct: 307 GFHGKPLRAGQEAYRTGMGSIELSAHNNVHTWSGD--PQQKYREDMGHFYAAARDPLFYA 366

Query: 361 HHGNVDRMWSIWRGLTD-VPRDYTDPDWLDSSFVFYDEHKKAVRITVRQCLESKDLGYVY 420
           HH NVDRMW+IW+GL D   RD+TDPDWL++SFVFYDE+K A+RI V+ CL+++ LGY Y
Sbjct: 367 HHANVDRMWTIWQGLIDGKSRDFTDPDWLNASFVFYDENKNAIRIKVQDCLDNEKLGYKY 426

Query: 421 QDVDIPWLYEKQIPRQRREALSAAGAPQVVSFPLSLDSQINASVERTPWIGK--DMGGAD 480
           Q V+IPWL+ K IPRQ+   L ++ A +     + L+S++   V+R             D
Sbjct: 427 QAVEIPWLHTKPIPRQKTSQLKSSKAQETPPIEV-LNSKMTKEVKRPQRSRSKTQKSVED 486

Query: 481 EVLVIEDIEFDSDSRVHFNVLVNVNALDDGADICPGNVEFAGNFTNIPHGHGHRVKTSLR 540
           EVLVIE +E D+ SRVHFNVLVNV  LDDG D+ PGN EFAG+F +IP GH HR  TSL 
Sbjct: 487 EVLVIE-MELDTTSRVHFNVLVNV--LDDGTDVSPGNTEFAGSFKSIPLGHKHRANTSLS 546

Query: 541 LVISDVIGELGLESNEELAVRIDPQVGGDKVTIGGIRIELQP 577
           LVI+++I ELG + +E + V IDP++GGD VTIGGI+IELQP
Sbjct: 547 LVITELIEELGYDDDETVFVTIDPKLGGDLVTIGGIKIELQP 572

BLAST of MS006101 vs. ExPASy TrEMBL
Match: A0A5A7UM88 (Polyphenol oxidase I OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold861G00520 PE=3 SV=1)

HSP 1 Score: 566.2 bits (1458), Expect = 1.5e-157
Identity = 293/579 (50.60%), Postives = 382/579 (65.98%), Query Frame = 0

Query: 2   LSLSSPYSRTKNQPSSLRNQRQ-GINYCVVPCRASNNGDQTPINSNPREAKISLNMFDRR 61
           L L SPYS+TK + S   N+R   +NY      +SNNG+             S++ FDRR
Sbjct: 7   LLLLSPYSQTKTKFSISSNKRHVVVNYVSCKAASSNNGED------------SVSKFDRR 66

Query: 62  DVLFGLGGLYSAATLGGNSSASASPQIPDVTICHQAIIEKPRTV-DCCPRPPFQADEITH 121
           +VLFGLGGLY   T  G  S  A+P  PDV  C  A  + P  + +CCP    +  +I H
Sbjct: 67  EVLFGLGGLYGVVTATG--SVLAAPLTPDVFNCDMA--QTPGGIKNCCPP---RIGKIIH 126

Query: 122 FVLPPATGKLRVRPAAHLMTPHQVAQFEKAIHIMKNCLDKDDPRHFCQQANVHCAYCNFA 181
           FV P        RPAAHLM   Q+ + E+ I  +K  LD+DDP  F  QA +HCAYC++A
Sbjct: 127 FVPPKIQQPYVRRPAAHLMNQTQIEELERGIQKLKQ-LDEDDPHSFSHQAKIHCAYCDYA 186

Query: 182 YKQ--EKYDVDFEVHWNALFFPFHRAYLYFFERICGHLLGDPDFAMPFWNWDSKPGMYFP 241
           Y Q     +  FEVH NALFFPFHRAYLYFFERI  H +G+PDFA+PFWNWDS PGM+ P
Sbjct: 187 YNQLGSITETRFEVHSNALFFPFHRAYLYFFERILRHYIGNPDFAIPFWNWDSPPGMHMP 246

Query: 242 QIYNDPNSPLYDKLRDARHTSGKLLDLNYNPNNEDPSYDVADCNVRWMNKQVLVNGNTAE 301
           + YN P+S LYD LRD RH   KL+DLNYN   ED S+DV DCN+RWMN ++ V   +  
Sbjct: 247 EFYNRPSSSLYDNLRDPRHDPHKLIDLNYNGKTEDHSFDVVDCNLRWMNDRMKV--QSPR 306

Query: 302 GFLGKLLRGGDEAEGKGMGSIERSPHNNVHDWIGDPPPNTKYGEDMGNFYSAGRDPVFYA 361
            FLG++L+ G+  +   MG+IE+SPHNN+HDW+G   P   + EDMG+FYSA  DP+FYA
Sbjct: 307 SFLGRVLKAGETPKPSDMGTIEQSPHNNIHDWVGTKTP---HFEDMGSFYSAALDPLFYA 366

Query: 362 HHGNVDRMWSIWRGLTDVPRDYTDPDWLDSSFVFYDEHKKAVRITVRQCLESKDLGYVYQ 421
           HH N+DR+W+IW+ L   P+D+TDPDWL+SSFVFYDE+K AVRI V  CL+++ LGYVYQ
Sbjct: 367 HHTNIDRLWNIWKTLDGNPKDHTDPDWLNSSFVFYDENKNAVRIEVSDCLDTEKLGYVYQ 426

Query: 422 DVDIPWLYEKQIPRQRREALSAAGAPQVVSFPLSLDSQINASVERTPWIGKDMGGADEVL 481
           +V +PWL   +IP       +    P +++FP +L+S I   V+R    G      D VL
Sbjct: 427 EVPLPWLLLNKIPSPNPWIRTEEPEPPIINFPQTLNSDITTMVDRPEEAGSG-EEVDVVL 486

Query: 482 VIEDIEFDSDSRVHFNVLVNVNALDDGADICPGNVEFAGNFTNIPHGHGHRVKTSLRLVI 541
           V+ED+E+D  +RVHF+VLVNV  +D+  D C   +EF G+F NIPHG  H  KTSLR VI
Sbjct: 487 VLEDVEYDPTTRVHFDVLVNV--VDEAQDRCAAFLEFVGSFKNIPHGLHHHDKTSLRFVI 546

Query: 542 SDVIGELGLESNEELAVRIDPQVGGDKVTIGGIRIELQP 577
           ++VI  LGLE ++ LAV+I+P VGG+ VT+GGI+I+L+P
Sbjct: 547 TEVIQGLGLERDKNLAVKIEPMVGGEGVTLGGIKIQLEP 557

BLAST of MS006101 vs. ExPASy TrEMBL
Match: A0A5A7UTU0 (Polyphenol oxidase I OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold861G00530 PE=3 SV=1)

HSP 1 Score: 557.0 bits (1434), Expect = 9.3e-155
Identity = 311/585 (53.16%), Postives = 386/585 (65.98%), Query Frame = 0

Query: 1   LLSLSSPYSRTKNQPSSL-RNQRQGINYCVVPCRASNNGDQTPINSNPREAKISLNMFDR 60
           LLS+ SPYS  K QPS +  N+R+G+N   V C+ASNNG+             S+N FDR
Sbjct: 7   LLSV-SPYSGCKIQPSVIPLNKRRGVNN-HVSCKASNNGED------------SVNKFDR 66

Query: 61  RDVLFGLGGLYSAATLGGNSSASAS--PQIPDVTICHQAIIEKPRTV----DCCPRPPFQ 120
           RD+LFGLGGLY  ATL G   A A      PDV  C      KP  V    DCCP    +
Sbjct: 67  RDMLFGLGGLYGVATLSGKDIALADDWSLSPDVHNCKPV---KPSAVGKKMDCCPP---K 126

Query: 121 ADEITHFVLPPATGKLRVRPAAHLMTPHQVAQFEKAIHIMKNCLDKDDPRHFCQQANVHC 180
             EI  F  PP     RVRPA HLMTP Q+A  EK I +MK  L +DDPR F  QA VHC
Sbjct: 127 IKEIIEFT-PPVVPTPRVRPAVHLMTPEQIADLEKGIQMMK-MLPEDDPRSFAHQAKVHC 186

Query: 181 AYCNFAYKQEKYDVDFEVHWNALFFPFHRAYLYFFERICGHLLGDPDFAMPFWNWDSKPG 240
           AYC+ AYKQ    +DF+VH NALFFPFHRAYLYFFERI  HLL  PDFA+PFWNWD+ PG
Sbjct: 187 AYCDDAYKQMDSTMDFQVHENALFFPFHRAYLYFFERILCHLLEKPDFAIPFWNWDAPPG 246

Query: 241 MYFPQIYNDPNSPLYDKLRDARHTSGKLLDLNYNPNN-EDPSYDVADCNVRWMNKQVLVN 300
           M  P+IY  P+S LYD+ RD  H   KLL+LNY     +DP  ++ D N+RWMN Q+ V 
Sbjct: 247 MCMPKIYTKPSSSLYDENRDKCHQPPKLLNLNYGGKEVKDP--EIVDFNLRWMNDQMSV- 306

Query: 301 GNTAEGFLGKLLRGGDEAEGKGMGSIERSPHNNVHDWIGDPPPNTKYGEDMGNFYSAGRD 360
             TA+ FLGK+++ G+  E  G GSIE SPHNN+H W+GDP   T +GEDMG+FYSAGRD
Sbjct: 307 -ETAKEFLGKVIKRGNGPE-NGSGSIETSPHNNIHIWVGDPEA-TIHGEDMGHFYSAGRD 366

Query: 361 PVFYAHHGNVDRMWSIWRGLTDVPRDYTDPDWLDSSFVFYDEHKKAVRITVRQCLESKDL 420
           P+FYAHH NVDRMW+IW+ + D PRD+TD DWL+SSFV YDE++KAVRI V QCL+SK L
Sbjct: 367 PLFYAHHANVDRMWNIWQKMKDRPRDHTDMDWLNSSFVLYDENRKAVRIKVSQCLDSKIL 426

Query: 421 GYVYQDVDIPWLYEKQIPRQRREALSAAGAPQVVSFPLSLDSQINASVERTPWIG-KDMG 480
           GYVYQDV IPW     I R ++E  S    P  + FP  L S I   V+R      ++  
Sbjct: 427 GYVYQDVPIPWKDTPPIRRPKQER-SLGNLPPTIDFPQILSSDITTMVDRPEVASPEERI 486

Query: 481 GADEVLVIEDIEFDSDSRVHFNVLVNVNALDDGADICPGNVEFAGNFTNIPHGHGHRVKT 540
             +EVLV+ED+E+D    +HF+VL+NV   D+G D+  G++EF G FTN+PHG     KT
Sbjct: 487 NKNEVLVLEDVEYDPTLPLHFDVLLNVG--DEGEDVRAGHIEFVGTFTNVPHGRTTYHKT 546

Query: 541 SLRLVISDVIGELGLESNEELAVRIDPQV-GGDKVTIGGIRIELQ 576
           SL  VI+  I +LGLE ++++AV+I P V GGD++ IGGI+I+L+
Sbjct: 547 SLHFVITKKIQQLGLEGDKKVAVKIVPNVNGGDRIKIGGIKIQLE 560