MS006074 (gene) Bitter gourd (TR) v1

BLAST of MS006074 vs. NCBI nr
Match: XP_022138251.1 (E3 ubiquitin-protein ligase MIEL1 [Momordica charantia])

HSP 1 Score: 598.2 bits (1541), Expect = 3.5e-167
Identity = 264/266 (99.25%), Postives = 266/266 (100.00%), Query Frame = 0

Query: 5   MEISADDRLDFGKMGYGCKHYRRRCKIRAPCCNEIYPCRHCHNEATSVMRPFERHELDRF 64
           MEISADDRLDFGKMGYGCKHYRRRCKIRAPCCNEIYPCRHCHNEATSVMRPFERHELDRF
Sbjct: 1   MEISADDRLDFGKMGYGCKHYRRRCKIRAPCCNEIYPCRHCHNEATSVMRPFERHELDRF 60

Query: 65  DVKQVVCAVCDTEQPVARVCTNCGVNMGEYFCEICKFYDDDIEKGQFHCDDCGICRVGGR 124
           DVKQVVCAVCDTEQPVARVCTNCGVNMGEYFCEICKFYDDDI+KGQFHCDDCGICRVGGR
Sbjct: 61  DVKQVVCAVCDTEQPVARVCTNCGVNMGEYFCEICKFYDDDIDKGQFHCDDCGICRVGGR 120

Query: 125 ENYFHCKKCGSCYSVGLRDNHSCIENSMQHHCPICYEYLFDSLKDVAVMKCGHTMHRECY 184
           ENYFHCKKCGSCYSVGLRDNHSCIENSMQHHCPICYEYLFDSLKDVAVMKCGHTMHRECY
Sbjct: 121 ENYFHCKKCGSCYSVGLRDNHSCIENSMQHHCPICYEYLFDSLKDVAVMKCGHTMHRECY 180

Query: 185 FEMMKRDKYCCPICSKSVVDMSKTWKRLDEEIEATVMPEEYRNKKVWILCNDCNDTTEVY 244
           FEM+KRDKYCCPICSKSVVDMSKTWKRLDEEIEATVMPEEYRNKKVWILCNDCNDTTEVY
Sbjct: 181 FEMIKRDKYCCPICSKSVVDMSKTWKRLDEEIEATVMPEEYRNKKVWILCNDCNDTTEVY 240

Query: 245 FHIIGQKCCHCQSYNTRAIAPPVLPQ 271
           FHIIGQKCCHCQSYNTRAIAPPVLPQ
Sbjct: 241 FHIIGQKCCHCQSYNTRAIAPPVLPQ 266

BLAST of MS006074 vs. NCBI nr
Match: XP_022979906.1 (E3 ubiquitin-protein ligase MIEL1-like [Cucurbita maxima] >XP_023528260.1 E3 ubiquitin-protein ligase MIEL1-like [Cucurbita pepo subsp. pepo] >XP_023528261.1 E3 ubiquitin-protein ligase MIEL1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 570.5 bits (1469), Expect = 7.9e-159
Identity = 250/267 (93.63%), Postives = 262/267 (98.13%), Query Frame = 0

Query: 5   MEISADDRLDFGKMGYGCKHYRRRCKIRAPCCNEIYPCRHCHNEATSVM-RPFERHELDR 64
           ME+SADDRLDFGKMGYGC+HYRRRC+IRA CCNEIYPCRHCHNEATSVM RPF+RHEL+R
Sbjct: 1   MEVSADDRLDFGKMGYGCQHYRRRCRIRASCCNEIYPCRHCHNEATSVMSRPFDRHELNR 60

Query: 65  FDVKQVVCAVCDTEQPVARVCTNCGVNMGEYFCEICKFYDDDIEKGQFHCDDCGICRVGG 124
           FDVKQVVCAVCDTEQPVARVCTNCGVNMGEYFCEICKF+DDDIEKGQFHC+DCGICRVGG
Sbjct: 61  FDVKQVVCAVCDTEQPVARVCTNCGVNMGEYFCEICKFFDDDIEKGQFHCEDCGICRVGG 120

Query: 125 RENYFHCKKCGSCYSVGLRDNHSCIENSMQHHCPICYEYLFDSLKDVAVMKCGHTMHREC 184
           RENYFHCKKCGSCYSV LRDNHSCIENSMQHHCPICYE+LFD+LKDVAVMKCGHTMHREC
Sbjct: 121 RENYFHCKKCGSCYSVNLRDNHSCIENSMQHHCPICYEFLFDTLKDVAVMKCGHTMHREC 180

Query: 185 YFEMMKRDKYCCPICSKSVVDMSKTWKRLDEEIEATVMPEEYRNKKVWILCNDCNDTTEV 244
           YFEM+KRDKYCCPICSKSV DMSK WK+LDEEIEATVMPEEYR+KKVWILCNDCNDTTEV
Sbjct: 181 YFEMIKRDKYCCPICSKSVTDMSKAWKQLDEEIEATVMPEEYRHKKVWILCNDCNDTTEV 240

Query: 245 YFHIIGQKCCHCQSYNTRAIAPPVLPQ 271
           YFHIIGQKCCHCQSYNTRAIAPPVLPQ
Sbjct: 241 YFHIIGQKCCHCQSYNTRAIAPPVLPQ 267

BLAST of MS006074 vs. NCBI nr
Match: KAG6582235.1 (E3 ubiquitin-protein ligase MIEL1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 568.9 bits (1465), Expect = 2.3e-158
Identity = 249/267 (93.26%), Postives = 261/267 (97.75%), Query Frame = 0

Query: 5   MEISADDRLDFGKMGYGCKHYRRRCKIRAPCCNEIYPCRHCHNEATSVM-RPFERHELDR 64
           ME+SADDRLDFGKMGYGC+HYRRRC+IRA CCNEIYPCRHCHNEATSVM RPF+RHEL+R
Sbjct: 1   MEVSADDRLDFGKMGYGCQHYRRRCRIRASCCNEIYPCRHCHNEATSVMSRPFDRHELNR 60

Query: 65  FDVKQVVCAVCDTEQPVARVCTNCGVNMGEYFCEICKFYDDDIEKGQFHCDDCGICRVGG 124
           FDVKQVVCAVCDTEQPVARVCTNCGVNMGEYFCEICKF+DDDIEKGQFHC+DCGICRVGG
Sbjct: 61  FDVKQVVCAVCDTEQPVARVCTNCGVNMGEYFCEICKFFDDDIEKGQFHCEDCGICRVGG 120

Query: 125 RENYFHCKKCGSCYSVGLRDNHSCIENSMQHHCPICYEYLFDSLKDVAVMKCGHTMHREC 184
           RENYFHCKKCGSCYSV LRDNHSCIENSMQHHCPICYE+LFD+LKDVAVMKCGHTMHREC
Sbjct: 121 RENYFHCKKCGSCYSVNLRDNHSCIENSMQHHCPICYEFLFDTLKDVAVMKCGHTMHREC 180

Query: 185 YFEMMKRDKYCCPICSKSVVDMSKTWKRLDEEIEATVMPEEYRNKKVWILCNDCNDTTEV 244
           YFEM+KRDKYCCPICSKSV DMSK WK+LDEEIEATVMPEEYR+KKVWILCNDCNDTTE 
Sbjct: 181 YFEMIKRDKYCCPICSKSVTDMSKAWKQLDEEIEATVMPEEYRHKKVWILCNDCNDTTEA 240

Query: 245 YFHIIGQKCCHCQSYNTRAIAPPVLPQ 271
           YFHIIGQKCCHCQSYNTRAIAPPVLPQ
Sbjct: 241 YFHIIGQKCCHCQSYNTRAIAPPVLPQ 267

BLAST of MS006074 vs. NCBI nr
Match: XP_022956148.1 (E3 ubiquitin-protein ligase MIEL1-like [Cucurbita moschata] >XP_022956149.1 E3 ubiquitin-protein ligase MIEL1-like [Cucurbita moschata])

HSP 1 Score: 567.4 bits (1461), Expect = 6.7e-158
Identity = 248/267 (92.88%), Postives = 261/267 (97.75%), Query Frame = 0

Query: 5   MEISADDRLDFGKMGYGCKHYRRRCKIRAPCCNEIYPCRHCHNEATSVM-RPFERHELDR 64
           ME+SADDRLDFGKMGYGC+HYRRRC+IRA CCNEIYPCRHCHNEA+SVM RPF+RHEL+R
Sbjct: 1   MEVSADDRLDFGKMGYGCQHYRRRCRIRASCCNEIYPCRHCHNEASSVMSRPFDRHELNR 60

Query: 65  FDVKQVVCAVCDTEQPVARVCTNCGVNMGEYFCEICKFYDDDIEKGQFHCDDCGICRVGG 124
           FDVKQVVCAVCDTEQPVARVCTNCGVNMGEYFCEICKF+DDDIEKGQFHC+DCGICRVGG
Sbjct: 61  FDVKQVVCAVCDTEQPVARVCTNCGVNMGEYFCEICKFFDDDIEKGQFHCEDCGICRVGG 120

Query: 125 RENYFHCKKCGSCYSVGLRDNHSCIENSMQHHCPICYEYLFDSLKDVAVMKCGHTMHREC 184
           RENYFHCKKCGSCYSV LRDNHSCIENSMQHHCPICYE+LFD+LKDVAVMKCGHTMHREC
Sbjct: 121 RENYFHCKKCGSCYSVNLRDNHSCIENSMQHHCPICYEFLFDTLKDVAVMKCGHTMHREC 180

Query: 185 YFEMMKRDKYCCPICSKSVVDMSKTWKRLDEEIEATVMPEEYRNKKVWILCNDCNDTTEV 244
           YFEM+KRDKYCCPICSKSV DMSK WK+LDEEIEATVMPEEYR+KKVWILCNDCNDTTE 
Sbjct: 181 YFEMIKRDKYCCPICSKSVTDMSKAWKQLDEEIEATVMPEEYRHKKVWILCNDCNDTTEA 240

Query: 245 YFHIIGQKCCHCQSYNTRAIAPPVLPQ 271
           YFHIIGQKCCHCQSYNTRAIAPPVLPQ
Sbjct: 241 YFHIIGQKCCHCQSYNTRAIAPPVLPQ 267

BLAST of MS006074 vs. NCBI nr
Match: XP_023540312.1 (E3 ubiquitin-protein ligase MIEL1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 566.6 bits (1459), Expect = 1.1e-157
Identity = 250/267 (93.63%), Postives = 259/267 (97.00%), Query Frame = 0

Query: 5   MEISADDRLDFGKMGYGCKHYRRRCKIRAPCCNEIYPCRHCHNEATSVM-RPFERHELDR 64
           ME+SADDR DFGKMGYGCKHYRRRCKIRAPCCNEIYPCRHCHNEATSVM RPF+RHEL+R
Sbjct: 1   MEVSADDRFDFGKMGYGCKHYRRRCKIRAPCCNEIYPCRHCHNEATSVMTRPFDRHELNR 60

Query: 65  FDVKQVVCAVCDTEQPVARVCTNCGVNMGEYFCEICKFYDDDIEKGQFHCDDCGICRVGG 124
           FDVKQVVCAVCDTEQPVARVCTNCGVNMGEYFCEICKFYDDDIEKGQFHC+DCGICRVGG
Sbjct: 61  FDVKQVVCAVCDTEQPVARVCTNCGVNMGEYFCEICKFYDDDIEKGQFHCEDCGICRVGG 120

Query: 125 RENYFHCKKCGSCYSVGLRDNHSCIENSMQHHCPICYEYLFDSLKDVAVMKCGHTMHREC 184
           +ENYFHCKKCGSCYSV LRDNHSCIENSMQHHCPICYEYLFDSLKDVAVMKCGHTMHREC
Sbjct: 121 QENYFHCKKCGSCYSVNLRDNHSCIENSMQHHCPICYEYLFDSLKDVAVMKCGHTMHREC 180

Query: 185 YFEMMKRDKYCCPICSKSVVDMSKTWKRLDEEIEATVMPEEYRNKKVWILCNDCNDTTEV 244
           YFEM+ RDKYCCPICSKSV+DMSK WK+LDEEIEATVMPEEYR+KKVWILCNDCNDTTE 
Sbjct: 181 YFEMIIRDKYCCPICSKSVIDMSKAWKQLDEEIEATVMPEEYRDKKVWILCNDCNDTTEA 240

Query: 245 YFHIIGQKCCHCQSYNTRAIAPPVLPQ 271
            FHIIGQKC HCQSYNTRAIAPPVLPQ
Sbjct: 241 CFHIIGQKCSHCQSYNTRAIAPPVLPQ 267

BLAST of MS006074 vs. ExPASy Swiss-Prot
Match: Q8VZK0 (E3 ubiquitin-protein ligase MIEL1 OS=Arabidopsis thaliana OX=3702 GN=MIEL1 PE=1 SV=1)

HSP 1 Score: 505.0 bits (1299), Expect = 5.3e-142
Identity = 216/267 (80.90%), Postives = 245/267 (91.76%), Query Frame = 0

Query: 5   MEISADDRLDFGKMGYGCKHYRRRCKIRAPCCNEIYPCRHCHNEATSVMRP-FERHELDR 64
           ME S +DRL FGKMG+GCKHY+RRC+IRAPCCNE++ CRHCHNE+TS +R  ++RH+L R
Sbjct: 1   MEASPNDRLHFGKMGFGCKHYKRRCQIRAPCCNEVFDCRHCHNESTSTLRNIYDRHDLVR 60

Query: 65  FDVKQVVCAVCDTEQPVARVCTNCGVNMGEYFCEICKFYDDDIEKGQFHCDDCGICRVGG 124
            DVKQV+C+VCDTEQP A+VC+NCGVNMGEYFC IC FYDDD EK QFHCDDCGICRVGG
Sbjct: 61  QDVKQVICSVCDTEQPAAQVCSNCGVNMGEYFCSICIFYDDDTEKQQFHCDDCGICRVGG 120

Query: 125 RENYFHCKKCGSCYSVGLRDNHSCIENSMQHHCPICYEYLFDSLKDVAVMKCGHTMHREC 184
           REN+FHCKKCGSCY+VGLR+NH C+ENSM+HHCPICYEYLFDSLKD  VMKCGHTMH EC
Sbjct: 121 RENFFHCKKCGSCYAVGLRNNHRCVENSMRHHCPICYEYLFDSLKDTNVMKCGHTMHVEC 180

Query: 185 YFEMMKRDKYCCPICSKSVVDMSKTWKRLDEEIEATVMPEEYRNKKVWILCNDCNDTTEV 244
           Y EM+KRDK+CCPICS+SV+DMSKTW+RLDEEIEAT MP +YR+KKVWILCNDCNDTTEV
Sbjct: 181 YNEMIKRDKFCCPICSRSVIDMSKTWQRLDEEIEATAMPSDYRDKKVWILCNDCNDTTEV 240

Query: 245 YFHIIGQKCCHCQSYNTRAIAPPVLPQ 271
           +FHIIGQKC HC+SYNTRAIAPPVLPQ
Sbjct: 241 HFHIIGQKCGHCRSYNTRAIAPPVLPQ 267

BLAST of MS006074 vs. ExPASy Swiss-Prot
Match: Q10LI1 (E3 ubiquitin-protein ligase SRFP1 OS=Oryza sativa subsp. japonica OX=39947 GN=SRFP1 PE=1 SV=1)

HSP 1 Score: 399.4 bits (1025), Expect = 3.1e-110
Identity = 167/265 (63.02%), Postives = 209/265 (78.87%), Query Frame = 0

Query: 5   MEISADDRLDFGKMGYGCKHYRRRCKIRAPCCNEIYPCRHCHNEATSVMRPFERHELDRF 64
           ME+    R  FG+MG+GCKHYRRRC+IRAPCCN+++ CRHCHNE+T      + HELDR 
Sbjct: 1   MELDDASRHGFGRMGFGCKHYRRRCRIRAPCCNDVFHCRHCHNESTK-----DGHELDRH 60

Query: 65  DVKQVVCAVCDTEQPVARVCTNCGVNMGEYFCEICKFYDDDIEKGQFHCDDCGICRVGGR 124
            V+ V+C VCDTEQPVA+VC NCGV MGEYFC  CKF+DDD+++  FHC DCGICRVGG+
Sbjct: 61  AVESVICLVCDTEQPVAQVCYNCGVCMGEYFCSACKFFDDDVDREHFHCQDCGICRVGGK 120

Query: 125 ENYFHCKKCGSCYSVGLRDNHSCIENSMQHHCPICYEYLFDSLKDVAVMKCGHTMHRECY 184
           +N+FHC+KCGSCYSV LRD H CIENSM+++CPICYEYLFDSL++ +V++CGHTMH +C+
Sbjct: 121 DNFFHCEKCGSCYSVSLRDKHCCIENSMKNNCPICYEYLFDSLRETSVLRCGHTMHLQCF 180

Query: 185 FEMMKRDKYCCPICSKSVVDMSKTWKRLDEEIEATVMPEEYRNKKVWILCNDCNDTTEVY 244
            EM+K DK+ CPICS  + DM K  + LD EIEA ++  +Y  K  WI+CNDC DTT+VY
Sbjct: 181 HEMLKHDKFSCPICSMPIFDMDKFLRALDAEIEANMLHIDYMGKG-WIVCNDCRDTTQVY 240

Query: 245 FHIIGQKCCHCQSYNTRAIAPPVLP 270
             + G KCCHCQS+NT  +A PVLP
Sbjct: 241 ARVAGHKCCHCQSHNTCRVAAPVLP 259

BLAST of MS006074 vs. ExPASy Swiss-Prot
Match: Q9FFB6 (E3 ubiquitin-protein ligase RZFP34 OS=Arabidopsis thaliana OX=3702 GN=RZPF34 PE=1 SV=1)

HSP 1 Score: 360.9 bits (925), Expect = 1.2e-98
Identity = 147/251 (58.57%), Postives = 186/251 (74.10%), Query Frame = 0

Query: 13  LDFGKMGYGCKHYRRRCKIRAPCCNEIYPCRHCHNEATSVM--RPFERHELDRFDVKQVV 72
           ++ G   YGC HYRRRCKIRAPCC+EI+ CRHCHNEA   +      RHEL R +V +V+
Sbjct: 18  MEIGSGHYGCSHYRRRCKIRAPCCDEIFDCRHCHNEAKDSLHIEQHHRHELPRHEVSKVI 77

Query: 73  CAVCDTEQPVARVCTNCGVNMGEYFCEICKFYDDDIEKGQFHCDDCGICRVGGRENYFHC 132
           C++C+TEQ V + C+NCGV MG+YFC  CKF+DDD+ K Q+HCD+CGICR GG EN+FHC
Sbjct: 78  CSLCETEQDVQQNCSNCGVCMGKYFCSKCKFFDDDLSKKQYHCDECGICRTGGEENFFHC 137

Query: 133 KKCGSCYSVGLRDNHSCIENSMQHHCPICYEYLFDSLKDVAVMKCGHTMHRECYFEMMKR 192
           K+C  CYS  + D H C+E +M H+CP+C+EYLFDS +D+ V++CGHTMH EC  +M   
Sbjct: 138 KRCRCCYSKIMEDKHQCVEGAMHHNCPVCFEYLFDSTRDITVLRCGHTMHLECTKDMGLH 197

Query: 193 DKYCCPICSKSVVDMSKTWKRLDEEIEATVMPEEYRNKKVWILCNDCNDTTEVYFHIIGQ 252
           ++Y CP+CSKS+ DMS  WK+LDEE+ A  MP+ Y NK VWILCNDC   T V FH+I  
Sbjct: 198 NRYTCPVCSKSICDMSNLWKKLDEEVAAYPMPKMYENKMVWILCNDCGSNTNVRFHLIAH 257

Query: 253 KCCHCQSYNTR 262
           KC  C SYNTR
Sbjct: 258 KCSSCGSYNTR 268

BLAST of MS006074 vs. ExPASy Swiss-Prot
Match: Q5JL96 (Probable E3 ubiquitin-protein ligase RZFP34 OS=Oryza sativa subsp. japonica OX=39947 GN=RZFP34 PE=2 SV=1)

HSP 1 Score: 352.4 bits (903), Expect = 4.4e-96
Identity = 149/262 (56.87%), Postives = 199/262 (75.95%), Query Frame = 0

Query: 6   EISADD--RLDFGKMGYGCKHYRRRCKIRAPCCNEIYPCRHCHNEATSVMR--PFERHEL 65
           ++  DD  +L+ G M YGC HYRRRC+IRAPCCNEI+ CRHCHNE  + ++    +RHEL
Sbjct: 43  DVDPDDYEKLEEGIMQYGCAHYRRRCRIRAPCCNEIFDCRHCHNETKNSIKIDAVKRHEL 102

Query: 66  DRFDVKQVVCAVCDTEQPVARVCTNCGVNMGEYFCEICKFYDDDIEKGQFHCDDCGICRV 125
            R +V+QV+C++C TEQ V +VC +CGV MG+YFCE+CK +DDD+ K Q+HC+ CGICR+
Sbjct: 103 PRHEVQQVICSLCGTEQEVRQVCISCGVCMGKYFCEVCKLFDDDVSKQQYHCNGCGICRI 162

Query: 126 GGRENYFHCKKCGSCYSVGLRDNHSCIENSMQHHCPICYEYLFDSLKDVAVMKCGHTMHR 185
           GG+EN+FHC KCG CYS+ L+++H+C+E +M H CPIC+EYLF+S  DV+V+ CGHT+H 
Sbjct: 163 GGKENFFHCSKCGCCYSIVLKNSHACVEGAMHHDCPICFEYLFESTNDVSVLPCGHTIHV 222

Query: 186 ECYFEMMKRDKYCCPICSKSVVDMSKTWKRLDEEIEATVMPEEYRNKKVWILCNDCNDTT 245
           +C  EM +  ++ CP+CSKSV DMSK W+RLDEE+    + +   NK V ILCNDC  T+
Sbjct: 223 KCLREMEEHCQFACPLCSKSVCDMSKAWERLDEEL--ATISDTCDNKMVRILCNDCGATS 282

Query: 246 EVYFHIIGQKCCHCQSYNTRAI 264
           EV FH+I  KC  C+SYNTR I
Sbjct: 283 EVQFHLIAHKCQKCKSYNTRQI 302

BLAST of MS006074 vs. ExPASy Swiss-Prot
Match: Q96PM5 (RING finger and CHY zinc finger domain-containing protein 1 OS=Homo sapiens OX=9606 GN=RCHY1 PE=1 SV=1)

HSP 1 Score: 263.5 bits (672), Expect = 2.7e-69
Identity = 114/240 (47.50%), Postives = 157/240 (65.42%), Query Frame = 0

Query: 21  GCKHYRRRCKIRAPCCNEIYPCRHCHNEATSVMRPFERHELDRFDVKQVVCAVCDTEQPV 80
           GC+HY R C ++APCC+++Y CR CH+         E H+LDRF VK+V C  C+  Q  
Sbjct: 19  GCEHYDRGCLLKAPCCDKLYTCRLCHDNN-------EDHQLDRFKVKEVQCINCEKIQHA 78

Query: 81  ARVCTNCGVNMGEYFCEICKFYDDDIEKGQFHCDDCGICRVGGRENYFHCKKCGSCYSVG 140
            + C  C    GEY+C+IC  +D D  K Q+HC++CGICR+G +E++FHC KC  C ++ 
Sbjct: 79  QQTCEECSTLFGEYYCDICHLFDKD--KKQYHCENCGICRIGPKEDFFHCLKCNLCLAMN 138

Query: 141 LRDNHSCIENSMQHHCPICYEYLFDSLKDVAVMKCGHTMHRECYFEMMKRDKYCCPICSK 200
           L+  H CIEN  + +CPIC E +  S     V+ CGH +HR CY EM+K + Y CP+C  
Sbjct: 139 LQGRHKCIENVSRQNCPICLEDIHTSRVVAHVLPCGHLLHRTCYEEMLK-EGYRCPLCMH 198

Query: 201 SVVDMSKTWKRLDEEIEATVMPEEYRNKKVWILCNDCNDTTEVYFHIIGQKCCHCQSYNT 260
           S +DM++ W++LD+E+  T MP EY+N  V ILCNDCN  + V FHI+G KC  C+SYNT
Sbjct: 199 SALDMTRYWRQLDDEVAQTPMPSEYQNMTVDILCNDCNGRSTVQFHILGMKCKICESYNT 248

BLAST of MS006074 vs. ExPASy TrEMBL
Match: A0A6J1C975 (E3 ubiquitin-protein ligase MIEL1 OS=Momordica charantia OX=3673 GN=LOC111009472 PE=4 SV=1)

HSP 1 Score: 598.2 bits (1541), Expect = 1.7e-167
Identity = 264/266 (99.25%), Postives = 266/266 (100.00%), Query Frame = 0

Query: 5   MEISADDRLDFGKMGYGCKHYRRRCKIRAPCCNEIYPCRHCHNEATSVMRPFERHELDRF 64
           MEISADDRLDFGKMGYGCKHYRRRCKIRAPCCNEIYPCRHCHNEATSVMRPFERHELDRF
Sbjct: 1   MEISADDRLDFGKMGYGCKHYRRRCKIRAPCCNEIYPCRHCHNEATSVMRPFERHELDRF 60

Query: 65  DVKQVVCAVCDTEQPVARVCTNCGVNMGEYFCEICKFYDDDIEKGQFHCDDCGICRVGGR 124
           DVKQVVCAVCDTEQPVARVCTNCGVNMGEYFCEICKFYDDDI+KGQFHCDDCGICRVGGR
Sbjct: 61  DVKQVVCAVCDTEQPVARVCTNCGVNMGEYFCEICKFYDDDIDKGQFHCDDCGICRVGGR 120

Query: 125 ENYFHCKKCGSCYSVGLRDNHSCIENSMQHHCPICYEYLFDSLKDVAVMKCGHTMHRECY 184
           ENYFHCKKCGSCYSVGLRDNHSCIENSMQHHCPICYEYLFDSLKDVAVMKCGHTMHRECY
Sbjct: 121 ENYFHCKKCGSCYSVGLRDNHSCIENSMQHHCPICYEYLFDSLKDVAVMKCGHTMHRECY 180

Query: 185 FEMMKRDKYCCPICSKSVVDMSKTWKRLDEEIEATVMPEEYRNKKVWILCNDCNDTTEVY 244
           FEM+KRDKYCCPICSKSVVDMSKTWKRLDEEIEATVMPEEYRNKKVWILCNDCNDTTEVY
Sbjct: 181 FEMIKRDKYCCPICSKSVVDMSKTWKRLDEEIEATVMPEEYRNKKVWILCNDCNDTTEVY 240

Query: 245 FHIIGQKCCHCQSYNTRAIAPPVLPQ 271
           FHIIGQKCCHCQSYNTRAIAPPVLPQ
Sbjct: 241 FHIIGQKCCHCQSYNTRAIAPPVLPQ 266

BLAST of MS006074 vs. ExPASy TrEMBL
Match: A0A6J1IS32 (E3 ubiquitin-protein ligase MIEL1-like OS=Cucurbita maxima OX=3661 GN=LOC111479459 PE=4 SV=1)

HSP 1 Score: 570.5 bits (1469), Expect = 3.8e-159
Identity = 250/267 (93.63%), Postives = 262/267 (98.13%), Query Frame = 0

Query: 5   MEISADDRLDFGKMGYGCKHYRRRCKIRAPCCNEIYPCRHCHNEATSVM-RPFERHELDR 64
           ME+SADDRLDFGKMGYGC+HYRRRC+IRA CCNEIYPCRHCHNEATSVM RPF+RHEL+R
Sbjct: 1   MEVSADDRLDFGKMGYGCQHYRRRCRIRASCCNEIYPCRHCHNEATSVMSRPFDRHELNR 60

Query: 65  FDVKQVVCAVCDTEQPVARVCTNCGVNMGEYFCEICKFYDDDIEKGQFHCDDCGICRVGG 124
           FDVKQVVCAVCDTEQPVARVCTNCGVNMGEYFCEICKF+DDDIEKGQFHC+DCGICRVGG
Sbjct: 61  FDVKQVVCAVCDTEQPVARVCTNCGVNMGEYFCEICKFFDDDIEKGQFHCEDCGICRVGG 120

Query: 125 RENYFHCKKCGSCYSVGLRDNHSCIENSMQHHCPICYEYLFDSLKDVAVMKCGHTMHREC 184
           RENYFHCKKCGSCYSV LRDNHSCIENSMQHHCPICYE+LFD+LKDVAVMKCGHTMHREC
Sbjct: 121 RENYFHCKKCGSCYSVNLRDNHSCIENSMQHHCPICYEFLFDTLKDVAVMKCGHTMHREC 180

Query: 185 YFEMMKRDKYCCPICSKSVVDMSKTWKRLDEEIEATVMPEEYRNKKVWILCNDCNDTTEV 244
           YFEM+KRDKYCCPICSKSV DMSK WK+LDEEIEATVMPEEYR+KKVWILCNDCNDTTEV
Sbjct: 181 YFEMIKRDKYCCPICSKSVTDMSKAWKQLDEEIEATVMPEEYRHKKVWILCNDCNDTTEV 240

Query: 245 YFHIIGQKCCHCQSYNTRAIAPPVLPQ 271
           YFHIIGQKCCHCQSYNTRAIAPPVLPQ
Sbjct: 241 YFHIIGQKCCHCQSYNTRAIAPPVLPQ 267

BLAST of MS006074 vs. ExPASy TrEMBL
Match: A0A6J1GVZ6 (E3 ubiquitin-protein ligase MIEL1-like OS=Cucurbita moschata OX=3662 GN=LOC111457930 PE=4 SV=1)

HSP 1 Score: 567.4 bits (1461), Expect = 3.2e-158
Identity = 248/267 (92.88%), Postives = 261/267 (97.75%), Query Frame = 0

Query: 5   MEISADDRLDFGKMGYGCKHYRRRCKIRAPCCNEIYPCRHCHNEATSVM-RPFERHELDR 64
           ME+SADDRLDFGKMGYGC+HYRRRC+IRA CCNEIYPCRHCHNEA+SVM RPF+RHEL+R
Sbjct: 1   MEVSADDRLDFGKMGYGCQHYRRRCRIRASCCNEIYPCRHCHNEASSVMSRPFDRHELNR 60

Query: 65  FDVKQVVCAVCDTEQPVARVCTNCGVNMGEYFCEICKFYDDDIEKGQFHCDDCGICRVGG 124
           FDVKQVVCAVCDTEQPVARVCTNCGVNMGEYFCEICKF+DDDIEKGQFHC+DCGICRVGG
Sbjct: 61  FDVKQVVCAVCDTEQPVARVCTNCGVNMGEYFCEICKFFDDDIEKGQFHCEDCGICRVGG 120

Query: 125 RENYFHCKKCGSCYSVGLRDNHSCIENSMQHHCPICYEYLFDSLKDVAVMKCGHTMHREC 184
           RENYFHCKKCGSCYSV LRDNHSCIENSMQHHCPICYE+LFD+LKDVAVMKCGHTMHREC
Sbjct: 121 RENYFHCKKCGSCYSVNLRDNHSCIENSMQHHCPICYEFLFDTLKDVAVMKCGHTMHREC 180

Query: 185 YFEMMKRDKYCCPICSKSVVDMSKTWKRLDEEIEATVMPEEYRNKKVWILCNDCNDTTEV 244
           YFEM+KRDKYCCPICSKSV DMSK WK+LDEEIEATVMPEEYR+KKVWILCNDCNDTTE 
Sbjct: 181 YFEMIKRDKYCCPICSKSVTDMSKAWKQLDEEIEATVMPEEYRHKKVWILCNDCNDTTEA 240

Query: 245 YFHIIGQKCCHCQSYNTRAIAPPVLPQ 271
           YFHIIGQKCCHCQSYNTRAIAPPVLPQ
Sbjct: 241 YFHIIGQKCCHCQSYNTRAIAPPVLPQ 267

BLAST of MS006074 vs. ExPASy TrEMBL
Match: A0A6J1GBT3 (E3 ubiquitin-protein ligase MIEL1 OS=Cucurbita moschata OX=3662 GN=LOC111452728 PE=4 SV=1)

HSP 1 Score: 565.8 bits (1457), Expect = 9.4e-158
Identity = 250/267 (93.63%), Postives = 260/267 (97.38%), Query Frame = 0

Query: 5   MEISADDRLDFGKMGYGCKHYRRRCKIRAPCCNEIYPCRHCHNEATSVM-RPFERHELDR 64
           ME+SADDR DFGKMGYGCKHYRRRCKIRAPCCNEIYPCRHCHNEATSVM RPF+RHEL+R
Sbjct: 1   MEVSADDRFDFGKMGYGCKHYRRRCKIRAPCCNEIYPCRHCHNEATSVMTRPFDRHELNR 60

Query: 65  FDVKQVVCAVCDTEQPVARVCTNCGVNMGEYFCEICKFYDDDIEKGQFHCDDCGICRVGG 124
           FDVKQVVCAVCDTEQPVARVCTNCGVNMGEYFCEICKFYDDDIEKGQFHC+DCGICRVGG
Sbjct: 61  FDVKQVVCAVCDTEQPVARVCTNCGVNMGEYFCEICKFYDDDIEKGQFHCEDCGICRVGG 120

Query: 125 RENYFHCKKCGSCYSVGLRDNHSCIENSMQHHCPICYEYLFDSLKDVAVMKCGHTMHREC 184
           +ENYFHCKKCGSCYSV LRDNHSCIENSMQHHCPICYEYLFDSLKDVAVMKCGHTMHREC
Sbjct: 121 QENYFHCKKCGSCYSVNLRDNHSCIENSMQHHCPICYEYLFDSLKDVAVMKCGHTMHREC 180

Query: 185 YFEMMKRDKYCCPICSKSVVDMSKTWKRLDEEIEATVMPEEYRNKKVWILCNDCNDTTEV 244
           YFEM+ R+KYCCPICSKSV+DMSK WK+LDEEIEATVMPEEYR+KKVWILCNDCNDTTEV
Sbjct: 181 YFEMIIREKYCCPICSKSVIDMSKAWKQLDEEIEATVMPEEYRDKKVWILCNDCNDTTEV 240

Query: 245 YFHIIGQKCCHCQSYNTRAIAPPVLPQ 271
            FHIIGQKC HCQSYNTRAIAPPVLPQ
Sbjct: 241 CFHIIGQKCNHCQSYNTRAIAPPVLPQ 267

BLAST of MS006074 vs. ExPASy TrEMBL
Match: A0A0A0LAI9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G151540 PE=4 SV=1)

HSP 1 Score: 558.9 bits (1439), Expect = 1.1e-155
Identity = 247/270 (91.48%), Postives = 257/270 (95.19%), Query Frame = 0

Query: 2   RLLMEISADDRLDFGKMGYGCKHYRRRCKIRAPCCNEIYPCRHCHNEATSVM-RPFERHE 61
           R  ME+SADDRLDFGKMGYGC+HYRRRCKIRAPCCNEIYPCRHCHNEATSVM R  +RHE
Sbjct: 2   RAFMEVSADDRLDFGKMGYGCQHYRRRCKIRAPCCNEIYPCRHCHNEATSVMSRLSDRHE 61

Query: 62  LDRFDVKQVVCAVCDTEQPVARVCTNCGVNMGEYFCEICKFYDDDIEKGQFHCDDCGICR 121
           L+RFDVKQVVCAVCDTEQPVARVCTNCGVNMGEYFCEICKFYDDDIEKGQFHC+DCGICR
Sbjct: 62  LNRFDVKQVVCAVCDTEQPVARVCTNCGVNMGEYFCEICKFYDDDIEKGQFHCEDCGICR 121

Query: 122 VGGRENYFHCKKCGSCYSVGLRDNHSCIENSMQHHCPICYEYLFDSLKDVAVMKCGHTMH 181
           VG RE YFHCKKCGSCY V LRDNHSCIENSMQHHCPICYEYLFD+LKDV+VMKCGHTMH
Sbjct: 122 VGSREKYFHCKKCGSCYHVNLRDNHSCIENSMQHHCPICYEYLFDTLKDVSVMKCGHTMH 181

Query: 182 RECYFEMMKRDKYCCPICSKSVVDMSKTWKRLDEEIEATVMPEEYRNKKVWILCNDCNDT 241
            ECY EM+ RDKYCCPICSKSVVDMSK WK+LDEEIEATVMPEEYR+KKVWILCNDCNDT
Sbjct: 182 LECYSEMINRDKYCCPICSKSVVDMSKAWKQLDEEIEATVMPEEYRHKKVWILCNDCNDT 241

Query: 242 TEVYFHIIGQKCCHCQSYNTRAIAPPVLPQ 271
           TEVYFHIIGQKCCHCQSYNTRAIAPPVLPQ
Sbjct: 242 TEVYFHIIGQKCCHCQSYNTRAIAPPVLPQ 271

BLAST of MS006074 vs. TAIR 10
Match: AT5G18650.1 (CHY-type/CTCHY-type/RING-type Zinc finger protein )

HSP 1 Score: 505.0 bits (1299), Expect = 3.8e-143
Identity = 216/267 (80.90%), Postives = 245/267 (91.76%), Query Frame = 0

Query: 5   MEISADDRLDFGKMGYGCKHYRRRCKIRAPCCNEIYPCRHCHNEATSVMRP-FERHELDR 64
           ME S +DRL FGKMG+GCKHY+RRC+IRAPCCNE++ CRHCHNE+TS +R  ++RH+L R
Sbjct: 1   MEASPNDRLHFGKMGFGCKHYKRRCQIRAPCCNEVFDCRHCHNESTSTLRNIYDRHDLVR 60

Query: 65  FDVKQVVCAVCDTEQPVARVCTNCGVNMGEYFCEICKFYDDDIEKGQFHCDDCGICRVGG 124
            DVKQV+C+VCDTEQP A+VC+NCGVNMGEYFC IC FYDDD EK QFHCDDCGICRVGG
Sbjct: 61  QDVKQVICSVCDTEQPAAQVCSNCGVNMGEYFCSICIFYDDDTEKQQFHCDDCGICRVGG 120

Query: 125 RENYFHCKKCGSCYSVGLRDNHSCIENSMQHHCPICYEYLFDSLKDVAVMKCGHTMHREC 184
           REN+FHCKKCGSCY+VGLR+NH C+ENSM+HHCPICYEYLFDSLKD  VMKCGHTMH EC
Sbjct: 121 RENFFHCKKCGSCYAVGLRNNHRCVENSMRHHCPICYEYLFDSLKDTNVMKCGHTMHVEC 180

Query: 185 YFEMMKRDKYCCPICSKSVVDMSKTWKRLDEEIEATVMPEEYRNKKVWILCNDCNDTTEV 244
           Y EM+KRDK+CCPICS+SV+DMSKTW+RLDEEIEAT MP +YR+KKVWILCNDCNDTTEV
Sbjct: 181 YNEMIKRDKFCCPICSRSVIDMSKTWQRLDEEIEATAMPSDYRDKKVWILCNDCNDTTEV 240

Query: 245 YFHIIGQKCCHCQSYNTRAIAPPVLPQ 271
           +FHIIGQKC HC+SYNTRAIAPPVLPQ
Sbjct: 241 HFHIIGQKCGHCRSYNTRAIAPPVLPQ 267

BLAST of MS006074 vs. TAIR 10
Match: AT3G62970.1 (zinc finger (C3HC4-type RING finger) family protein )

HSP 1 Score: 386.7 bits (992), Expect = 1.5e-107
Identity = 168/257 (65.37%), Postives = 206/257 (80.16%), Query Frame = 0

Query: 14  DFGKMGYGCKHYRRRCKIRAPCCNEIYPCRHCHNE-ATSVMRPFERHELDRFDVKQVVCA 73
           DFGK  +GC+HY+RRCKIRAPCCN I+ CRHCHN+ A S+  P ERH+L R +VKQVVC+
Sbjct: 27  DFGKFQFGCEHYKRRCKIRAPCCNLIFSCRHCHNDSANSLPDPKERHDLVRQNVKQVVCS 86

Query: 74  VCDTEQPVARVCTNCGVNMGEYFCEICKFYDDDIEKGQFHCDDCGICRVGGRENYFHCKK 133
           +C TEQ VA+VC+NCGVNMGEYFC+ICKF+DDDI K QFHCDDCGICRVGGR+ +FHC+ 
Sbjct: 87  ICQTEQEVAKVCSNCGVNMGEYFCDICKFFDDDISKEQFHCDDCGICRVGGRDKFFHCQN 146

Query: 134 CGSCYSVGLRDNHSCIENSMQHHCPICYEYLFDSLKDVAVMKCGHTMHRECYFEMMKRDK 193
           CG+CY +GLRD HSCIENS ++ CP+CYEYLFDS+K   VMKCGHTMH +C+ +M+  ++
Sbjct: 147 CGACYGMGLRDKHSCIENSTKNSCPVCYEYLFDSVKAAHVMKCGHTMHMDCFEQMINENQ 206

Query: 194 YCCPICSKSVVDMSKTWKRLDEEIEATVMPEEYRNKKVWILCNDCNDTTEVYFHIIGQKC 253
           Y CPIC+KS+VDMS +W  LD EI AT MP EY+  +V ILCNDCN  ++  FHI+G KC
Sbjct: 207 YRCPICAKSMVDMSPSWHLLDFEISATEMPVEYK-FEVSILCNDCNKGSKAMFHILGHKC 266

Query: 254 CHCQSYNTRAIAPPVLP 270
             C SYNTR I+ P  P
Sbjct: 267 SDCGSYNTRRISTPQDP 282

BLAST of MS006074 vs. TAIR 10
Match: AT5G22920.1 (CHY-type/CTCHY-type/RING-type Zinc finger protein )

HSP 1 Score: 360.9 bits (925), Expect = 8.8e-100
Identity = 147/251 (58.57%), Postives = 186/251 (74.10%), Query Frame = 0

Query: 13  LDFGKMGYGCKHYRRRCKIRAPCCNEIYPCRHCHNEATSVM--RPFERHELDRFDVKQVV 72
           ++ G   YGC HYRRRCKIRAPCC+EI+ CRHCHNEA   +      RHEL R +V +V+
Sbjct: 18  MEIGSGHYGCSHYRRRCKIRAPCCDEIFDCRHCHNEAKDSLHIEQHHRHELPRHEVSKVI 77

Query: 73  CAVCDTEQPVARVCTNCGVNMGEYFCEICKFYDDDIEKGQFHCDDCGICRVGGRENYFHC 132
           C++C+TEQ V + C+NCGV MG+YFC  CKF+DDD+ K Q+HCD+CGICR GG EN+FHC
Sbjct: 78  CSLCETEQDVQQNCSNCGVCMGKYFCSKCKFFDDDLSKKQYHCDECGICRTGGEENFFHC 137

Query: 133 KKCGSCYSVGLRDNHSCIENSMQHHCPICYEYLFDSLKDVAVMKCGHTMHRECYFEMMKR 192
           K+C  CYS  + D H C+E +M H+CP+C+EYLFDS +D+ V++CGHTMH EC  +M   
Sbjct: 138 KRCRCCYSKIMEDKHQCVEGAMHHNCPVCFEYLFDSTRDITVLRCGHTMHLECTKDMGLH 197

Query: 193 DKYCCPICSKSVVDMSKTWKRLDEEIEATVMPEEYRNKKVWILCNDCNDTTEVYFHIIGQ 252
           ++Y CP+CSKS+ DMS  WK+LDEE+ A  MP+ Y NK VWILCNDC   T V FH+I  
Sbjct: 198 NRYTCPVCSKSICDMSNLWKKLDEEVAAYPMPKMYENKMVWILCNDCGSNTNVRFHLIAH 257

Query: 253 KCCHCQSYNTR 262
           KC  C SYNTR
Sbjct: 258 KCSSCGSYNTR 268

BLAST of MS006074 vs. TAIR 10
Match: AT5G25560.1 (CHY-type/CTCHY-type/RING-type Zinc finger protein )

HSP 1 Score: 352.4 bits (903), Expect = 3.1e-97
Identity = 148/262 (56.49%), Postives = 191/262 (72.90%), Query Frame = 0

Query: 2   RLLMEISADDRLDFGKMGYGCKHYRRRCKIRAPCCNEIYPCRHCHNEATSVMR--PFERH 61
           R+  E   +  LD G M YGC HYRRRC IRAPCCNEI+ C HCH EA + +     +RH
Sbjct: 43  RVAAESLTNKVLDRGLMEYGCPHYRRRCCIRAPCCNEIFGCHHCHYEAKNNINVDQKQRH 102

Query: 62  ELDRFDVKQVVCAVCDTEQPVARVCTNCGVNMGEYFCEICKFYDDDIEKGQFHCDDCGIC 121
           ++ R  V+QV+C +C TEQ V ++C +CGV MG+YFC++CK YDDD  K Q+HCD CGIC
Sbjct: 103 DIPRHQVEQVICLLCGTEQEVGQICIHCGVCMGKYFCKVCKLYDDDTSKKQYHCDGCGIC 162

Query: 122 RVGGRENYFHCKKCGSCYSVGLRDNHSCIENSMQHHCPICYEYLFDSLKDVAVMKCGHTM 181
           R+GGREN+FHC KCG CYS+ L++ H C+E +M H CPIC+E+LF+S  DV V+ CGHT+
Sbjct: 163 RIGGRENFFHCYKCGCCYSILLKNGHPCVEGAMHHDCPICFEFLFESRNDVTVLPCGHTI 222

Query: 182 HRECYFEMMKRDKYCCPICSKSVVDMSKTWKRLDEEIEATVMPEEYRNKKVWILCNDCND 241
           H++C  EM    +Y CP+CSKSV DMSK W++ D EI AT MPE Y+N+ V ILCNDC  
Sbjct: 223 HQKCLEEMRDHYQYACPLCSKSVCDMSKVWEKFDMEIAATPMPEPYQNRMVQILCNDCGK 282

Query: 242 TTEVYFHIIGQKCCHCQSYNTR 262
             EV +H++ QKC +C+SYNTR
Sbjct: 283 KAEVQYHVVAQKCPNCKSYNTR 304

BLAST of MS006074 vs. TAIR 10
Match: AT5G25560.3 (CHY-type/CTCHY-type/RING-type Zinc finger protein )

HSP 1 Score: 344.4 bits (882), Expect = 8.5e-95
Identity = 148/272 (54.41%), Postives = 191/272 (70.22%), Query Frame = 0

Query: 2   RLLMEISADDRLDFGKMGYGCKHYRRRCKIRAPCCNEIYPCRHCHNEATSVMR--PFERH 61
           R+  E   +  LD G M YGC HYRRRC IRAPCCNEI+ C HCH EA + +     +RH
Sbjct: 43  RVAAESLTNKVLDRGLMEYGCPHYRRRCCIRAPCCNEIFGCHHCHYEAKNNINVDQKQRH 102

Query: 62  ELDRFDVK----------QVVCAVCDTEQPVARVCTNCGVNMGEYFCEICKFYDDDIEKG 121
           ++ R  V+          QV+C +C TEQ V ++C +CGV MG+YFC++CK YDDD  K 
Sbjct: 103 DIPRHQVEQLTRPLSSSSQVICLLCGTEQEVGQICIHCGVCMGKYFCKVCKLYDDDTSKK 162

Query: 122 QFHCDDCGICRVGGRENYFHCKKCGSCYSVGLRDNHSCIENSMQHHCPICYEYLFDSLKD 181
           Q+HCD CGICR+GGREN+FHC KCG CYS+ L++ H C+E +M H CPIC+E+LF+S  D
Sbjct: 163 QYHCDGCGICRIGGRENFFHCYKCGCCYSILLKNGHPCVEGAMHHDCPICFEFLFESRND 222

Query: 182 VAVMKCGHTMHRECYFEMMKRDKYCCPICSKSVVDMSKTWKRLDEEIEATVMPEEYRNKK 241
           V V+ CGHT+H++C  EM    +Y CP+CSKSV DMSK W++ D EI AT MPE Y+N+ 
Sbjct: 223 VTVLPCGHTIHQKCLEEMRDHYQYACPLCSKSVCDMSKVWEKFDMEIAATPMPEPYQNRM 282

Query: 242 VWILCNDCNDTTEVYFHIIGQKCCHCQSYNTR 262
           V ILCNDC    EV +H++ QKC +C+SYNTR
Sbjct: 283 VQILCNDCGKKAEVQYHVVAQKCPNCKSYNTR 314