MS005643 (gene) Bitter gourd (TR) v1

Overview
NameMS005643
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
Descriptionheat stress transcription factor A-5
Locationscaffold254: 500841 .. 502949 (+)
RNA-Seq ExpressionMS005643
SyntenyMS005643
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGGCAGCTGCTGGAGGCGGAGGCGCCGGCGGTGCTGGTCCGGCGCCGTTTCTCATAAAGACATACGACATGGTGGACGACTCGGCCACGGACTCGATCGTATCCTGGACCTCTTCGAACAAGAGCTTCGTGGTCTGGAATCCTCCTGAATTCGCTCGCCTTCTTCTTCCTACGTATTTCAAGCATAATAACTTCTCCAGCTTCATTCGGCAGCTTAATACCTATGTAAGTTTCCGGAAATTCTCATCTCGAGGGTAATTTTGGCGTTTCCGTGACTTGATTTTGTTCCTCCTGTTAATTGGAGACGGAGCATGTTCCTTATTGCGTAATTATGATTTTCTTATTGTTGTTTACCTCATTAGGACGATATGTATATATGTGTTTATGCATTCGTCTCATGGCTTGCAGCAATTCCTTATAGTTCAGTATTTACGAGCTTCAATTCATGATTTAACTATTTACGAACCCACGTGTTTTGTTGATGAATGTAATTTCGTTTGTTAATTCTGTTCTTTTATGCTTCGAGATTCTTATATCGGTTTCTTTGAAAAATTAACTCGATCTCCGAGGGAAGAATTCTGGAAAGGCTCAAGCAACTCAGATCTCTCTCTAGTTGAGCTACTACTAAATAATTCTCTCCACTTCCATTTGCGCTCCCAACTTATTATTATGCGATTCATGAACATGTAAAGGAAGAACTATGGGTGGAATTGGTTCGAAGGGTGGTACATAAATGGTTTCGTTGCATCTGTATGGTCATTATGTGCTGGTTATGATCATTCCTATATCCTAGAGTCTTTTGAATGATCAATATTGTAGACCACATTTTATAAGAAAACTATCATATTCTCTCGTTTCCAAATTTTGAAGTGAAAACGGTATTAAAAAGTAGCCAAAACTCACAAGTTTTAATTTTGGTGGCAGGGGTTTCGGAAGATTGATTCCGAGAGATGGGAATTTGCTAACGAAGACTTCATAAAGGATCAAAAGCATCTACTAAAGAACATCCATCGTCGGAAACCAATTCATAGTCACAGTAATCCTCAAGGATCTCTTGTAGATCCAGAAAGAGCTGCTTTTGAAGATGAAATAGAGAGGCTATCACGTGAAAAGGCTACACTTGAAGCTAATATCTCAAGGTTCAAACAGCAAAAATCCACTGCTAAACATCAGGTGCGAGATCTAACGGTGAAGGTGGAAAGCATGGAAAAAAGGCAGAAGAGTTTATTGGCATTTTTAGAAAAGGCTGTTCAAAACCCATCTTTTGTTGAACATCTTGCTCGAAGAGTCGAGTCTATGGACTTCACAGCATTTAACAAAAAGAGACGACTGCCTCAAGCTGATCATCCACAGCCACTCATTGAAAATAGTTTTTTGGACAACCATAGCAGTTCCAGGTCTGAGTCTGGCAATATTTTTCATCAGGATTTCTCACATAAGCTGAGGTTAGAATCATCAACCGTCTCGGATGTTAACTTAATTTCATGTAGCACCCAGAGTTCAAACGAAGAAGGGGGAAGTTCACAAAGAAATATGTCAAAAATAGATACTAGAGGCGTCCAAGAGAATCTTCATTTTGCAACCGAAACATTAGATCTCTCAGACACTGGGACATCATTTATATTGAAGAGGGATTCATCTTTGTCAGGGAAATCGCCTAACGACGAGAGCCCACATCTGCACTCGTCGCAACCAAGTGCTAGTTCCAAAGAAGATGGAGAAAGTCACATTTCCTGTCATTTAAATCTCACTCTTGCATCTTCTTCCCTGCGCGTCAACGACACTGCTTGTTCAGTTAGAATGCCACAACTGGGTCAGACTGCTCGAAAGTCTCCAGATTCAAATGGAAAAGAAGCAGACATGAGACTCTTCACTAAGAACATAAATCTTGATGAAAGAACAACGCCATTTTGTCCCCAAGAGAACCTAAGTAACCATCACGGGCCCTCAGCCGCAGCCGCAGCCGCTCCTGTCAGAGCCAATGATTTCTTCTGGGAACAGTTTCTTACTGAAAGACCAGGTTGTCCTGAAAGTGAAGAGGCAAGTTCTAATTACAGGGCAACTTTATACAAGGAGCCTGATGATGGGAGATCGGGCCTGTCTCTT

mRNA sequence

ATGGAGGCAGCTGCTGGAGGCGGAGGCGCCGGCGGTGCTGGTCCGGCGCCGTTTCTCATAAAGACATACGACATGGTGGACGACTCGGCCACGGACTCGATCGTATCCTGGACCTCTTCGAACAAGAGCTTCGTGGTCTGGAATCCTCCTGAATTCGCTCGCCTTCTTCTTCCTACGTATTTCAAGCATAATAACTTCTCCAGCTTCATTCGGCAGCTTAATACCTATGGGTTTCGGAAGATTGATTCCGAGAGATGGGAATTTGCTAACGAAGACTTCATAAAGGATCAAAAGCATCTACTAAAGAACATCCATCGTCGGAAACCAATTCATAGTCACAGTAATCCTCAAGGATCTCTTGTAGATCCAGAAAGAGCTGCTTTTGAAGATGAAATAGAGAGGCTATCACGTGAAAAGGCTACACTTGAAGCTAATATCTCAAGGTTCAAACAGCAAAAATCCACTGCTAAACATCAGGTGCGAGATCTAACGGTGAAGGTGGAAAGCATGGAAAAAAGGCAGAAGAGTTTATTGGCATTTTTAGAAAAGGCTGTTCAAAACCCATCTTTTGTTGAACATCTTGCTCGAAGAGTCGAGTCTATGGACTTCACAGCATTTAACAAAAAGAGACGACTGCCTCAAGCTGATCATCCACAGCCACTCATTGAAAATAGTTTTTTGGACAACCATAGCAGTTCCAGGTCTGAGTCTGGCAATATTTTTCATCAGGATTTCTCACATAAGCTGAGGTTAGAATCATCAACCGTCTCGGATGTTAACTTAATTTCATGTAGCACCCAGAGTTCAAACGAAGAAGGGGGAAGTTCACAAAGAAATATGTCAAAAATAGATACTAGAGGCGTCCAAGAGAATCTTCATTTTGCAACCGAAACATTAGATCTCTCAGACACTGGGACATCATTTATATTGAAGAGGGATTCATCTTTGTCAGGGAAATCGCCTAACGACGAGAGCCCACATCTGCACTCGTCGCAACCAAGTGCTAGTTCCAAAGAAGATGGAGAAAGTCACATTTCCTGTCATTTAAATCTCACTCTTGCATCTTCTTCCCTGCGCGTCAACGACACTGCTTGTTCAGTTAGAATGCCACAACTGGGTCAGACTGCTCGAAAGTCTCCAGATTCAAATGGAAAAGAAGCAGACATGAGACTCTTCACTAAGAACATAAATCTTGATGAAAGAACAACGCCATTTTGTCCCCAAGAGAACCTAAGTAACCATCACGGGCCCTCAGCCGCAGCCGCAGCCGCTCCTGTCAGAGCCAATGATTTCTTCTGGGAACAGTTTCTTACTGAAAGACCAGGTTGTCCTGAAAGTGAAGAGGCAAGTTCTAATTACAGGGCAACTTTATACAAGGAGCCTGATGATGGGAGATCGGGCCTGTCTCTT

Coding sequence (CDS)

ATGGAGGCAGCTGCTGGAGGCGGAGGCGCCGGCGGTGCTGGTCCGGCGCCGTTTCTCATAAAGACATACGACATGGTGGACGACTCGGCCACGGACTCGATCGTATCCTGGACCTCTTCGAACAAGAGCTTCGTGGTCTGGAATCCTCCTGAATTCGCTCGCCTTCTTCTTCCTACGTATTTCAAGCATAATAACTTCTCCAGCTTCATTCGGCAGCTTAATACCTATGGGTTTCGGAAGATTGATTCCGAGAGATGGGAATTTGCTAACGAAGACTTCATAAAGGATCAAAAGCATCTACTAAAGAACATCCATCGTCGGAAACCAATTCATAGTCACAGTAATCCTCAAGGATCTCTTGTAGATCCAGAAAGAGCTGCTTTTGAAGATGAAATAGAGAGGCTATCACGTGAAAAGGCTACACTTGAAGCTAATATCTCAAGGTTCAAACAGCAAAAATCCACTGCTAAACATCAGGTGCGAGATCTAACGGTGAAGGTGGAAAGCATGGAAAAAAGGCAGAAGAGTTTATTGGCATTTTTAGAAAAGGCTGTTCAAAACCCATCTTTTGTTGAACATCTTGCTCGAAGAGTCGAGTCTATGGACTTCACAGCATTTAACAAAAAGAGACGACTGCCTCAAGCTGATCATCCACAGCCACTCATTGAAAATAGTTTTTTGGACAACCATAGCAGTTCCAGGTCTGAGTCTGGCAATATTTTTCATCAGGATTTCTCACATAAGCTGAGGTTAGAATCATCAACCGTCTCGGATGTTAACTTAATTTCATGTAGCACCCAGAGTTCAAACGAAGAAGGGGGAAGTTCACAAAGAAATATGTCAAAAATAGATACTAGAGGCGTCCAAGAGAATCTTCATTTTGCAACCGAAACATTAGATCTCTCAGACACTGGGACATCATTTATATTGAAGAGGGATTCATCTTTGTCAGGGAAATCGCCTAACGACGAGAGCCCACATCTGCACTCGTCGCAACCAAGTGCTAGTTCCAAAGAAGATGGAGAAAGTCACATTTCCTGTCATTTAAATCTCACTCTTGCATCTTCTTCCCTGCGCGTCAACGACACTGCTTGTTCAGTTAGAATGCCACAACTGGGTCAGACTGCTCGAAAGTCTCCAGATTCAAATGGAAAAGAAGCAGACATGAGACTCTTCACTAAGAACATAAATCTTGATGAAAGAACAACGCCATTTTGTCCCCAAGAGAACCTAAGTAACCATCACGGGCCCTCAGCCGCAGCCGCAGCCGCTCCTGTCAGAGCCAATGATTTCTTCTGGGAACAGTTTCTTACTGAAAGACCAGGTTGTCCTGAAAGTGAAGAGGCAAGTTCTAATTACAGGGCAACTTTATACAAGGAGCCTGATGATGGGAGATCGGGCCTGTCTCTT

Protein sequence

MEAAAGGGGAGGAGPAPFLIKTYDMVDDSATDSIVSWTSSNKSFVVWNPPEFARLLLPTYFKHNNFSSFIRQLNTYGFRKIDSERWEFANEDFIKDQKHLLKNIHRRKPIHSHSNPQGSLVDPERAAFEDEIERLSREKATLEANISRFKQQKSTAKHQVRDLTVKVESMEKRQKSLLAFLEKAVQNPSFVEHLARRVESMDFTAFNKKRRLPQADHPQPLIENSFLDNHSSSRSESGNIFHQDFSHKLRLESSTVSDVNLISCSTQSSNEEGGSSQRNMSKIDTRGVQENLHFATETLDLSDTGTSFILKRDSSLSGKSPNDESPHLHSSQPSASSKEDGESHISCHLNLTLASSSLRVNDTACSVRMPQLGQTARKSPDSNGKEADMRLFTKNINLDERTTPFCPQENLSNHHGPSAAAAAAPVRANDFFWEQFLTERPGCPESEEASSNYRATLYKEPDDGRSGLSL
Homology
BLAST of MS005643 vs. NCBI nr
Match: XP_022146890.1 (heat stress transcription factor A-5 [Momordica charantia])

HSP 1 Score: 906.0 bits (2340), Expect = 1.4e-259
Identity = 467/470 (99.36%), Postives = 467/470 (99.36%), Query Frame = 0

Query: 1   MEAAAGGGGAGGAGPAPFLIKTYDMVDDSATDSIVSWTSSNKSFVVWNPPEFARLLLPTY 60
           MEAAAGGGGAGGAGPAPFLIKTYDMVDDSATDSIVSWTSSNKSFVVWNPPEFARLLLPTY
Sbjct: 1   MEAAAGGGGAGGAGPAPFLIKTYDMVDDSATDSIVSWTSSNKSFVVWNPPEFARLLLPTY 60

Query: 61  FKHNNFSSFIRQLNTYGFRKIDSERWEFANEDFIKDQKHLLKNIHRRKPIHSHSNPQGSL 120
           FKHNNFSSFIRQLNTYGFRKIDSERWEFANEDFIKDQKHLLKNIHRRKPIHSHSNPQGSL
Sbjct: 61  FKHNNFSSFIRQLNTYGFRKIDSERWEFANEDFIKDQKHLLKNIHRRKPIHSHSNPQGSL 120

Query: 121 VDPERAAFEDEIERLSREKATLEANISRFKQQKSTAKHQVRDLTVKVESMEKRQKSLLAF 180
           VDPERAAFEDEIERLSREKATLEANISRFKQQKSTAKHQVRDLTVKVESMEKRQKSLLAF
Sbjct: 121 VDPERAAFEDEIERLSREKATLEANISRFKQQKSTAKHQVRDLTVKVESMEKRQKSLLAF 180

Query: 181 LEKAVQNPSFVEHLARRVESMDFTAFNKKRRLPQADHPQPLIENSFLDNHSSSRSESGNI 240
           LEKAVQNPSFVEHLARRVESMDFTAFNKKRRLPQADHPQPLIENSFLDNHSSSRSESGNI
Sbjct: 181 LEKAVQNPSFVEHLARRVESMDFTAFNKKRRLPQADHPQPLIENSFLDNHSSSRSESGNI 240

Query: 241 FHQDFSHKLRLESSTVSDVNLISCSTQSSNEEGGSSQRNMSKIDTRGVQENLHFATETLD 300
           FHQDFSHKLRLESSTVSDVNLISCSTQSSNEEGGSSQRNMSKIDTRGVQENLHFATETLD
Sbjct: 241 FHQDFSHKLRLESSTVSDVNLISCSTQSSNEEGGSSQRNMSKIDTRGVQENLHFATETLD 300

Query: 301 LSDTGTSFILKRDSSLSGKSPNDESPHLHSSQPSASSKEDGESHISCHLNLTLASSSLRV 360
           LSDTGTSFILKRDSSLSGKSPNDESP L  SQPSASSKEDGESHISCHLNLTLASSSLRV
Sbjct: 301 LSDTGTSFILKRDSSLSGKSPNDESPRL--SQPSASSKEDGESHISCHLNLTLASSSLRV 360

Query: 361 NDTACSVRMPQLGQTARKSPDSNGKEADMRLFTKNINLDERTTPFCPQENLSNHHGPSAA 420
           NDTACSVRMPQLGQTARKSPDSNGKEADMRLFTKNINLDERTTPFCPQENLSNHHGPSAA
Sbjct: 361 NDTACSVRMPQLGQTARKSPDSNGKEADMRLFTKNINLDERTTPFCPQENLSNHHGPSAA 420

Query: 421 AAAAPVRANDFFWEQFLTERPGCPESEEASSNYRATLYKEPDDGRSGLSL 471
           AAAAPVRANDFFWEQFLTERPGCPESEEASSNYRATLYKEPDDGRSGLSL
Sbjct: 421 AAAAPVRANDFFWEQFLTERPGCPESEEASSNYRATLYKEPDDGRSGLSL 468

BLAST of MS005643 vs. NCBI nr
Match: XP_022974752.1 (heat stress transcription factor A-5-like [Cucurbita maxima] >XP_022974753.1 heat stress transcription factor A-5-like [Cucurbita maxima] >XP_022974754.1 heat stress transcription factor A-5-like [Cucurbita maxima] >XP_022974755.1 heat stress transcription factor A-5-like [Cucurbita maxima] >XP_022974756.1 heat stress transcription factor A-5-like [Cucurbita maxima])

HSP 1 Score: 784.3 bits (2024), Expect = 6.0e-223
Identity = 406/473 (85.84%), Postives = 431/473 (91.12%), Query Frame = 0

Query: 1   MEAAAGGGGAGGAGPAPFLIKTYDMVDDSATDSIVSWTSSNKSFVVWNPPEFARLLLPTY 60
           MEAAAGGGG GGAGPAPFLIKTYDMVD+SATD IVSWTSS KSFVVWNPPEFARLLLPT+
Sbjct: 1   MEAAAGGGGTGGAGPAPFLIKTYDMVDESATDEIVSWTSSKKSFVVWNPPEFARLLLPTF 60

Query: 61  FKHNNFSSFIRQLNTYGFRKIDSERWEFANEDFIKDQKHLLKNIHRRKPIHSHSNPQGSL 120
           FKHNNFSSFIRQLNTYGF+KIDSERWEFAN+DFIKDQKHLLKNIHRRKPIHSHS P GSL
Sbjct: 61  FKHNNFSSFIRQLNTYGFKKIDSERWEFANDDFIKDQKHLLKNIHRRKPIHSHSTPPGSL 120

Query: 121 VDPERAAFEDEIERLSREKATLEANISRFKQQKSTAKHQVRDLTVKVESMEKRQKSLLAF 180
           VDPERAAFEDEI+RLSR+K TLEA+ISRFKQQKSTAK Q++DLTVKVESMEKRQK+LLAF
Sbjct: 121 VDPERAAFEDEIDRLSRKKTTLEADISRFKQQKSTAKIQLQDLTVKVESMEKRQKNLLAF 180

Query: 181 LEKAVQNPSFVEHLARRVESMDFTAFNKKRRLPQADHPQPLIENSFLDNHSSSRSESGNI 240
           LEKAVQNPSFVEHLARRVESMDFTAFNKKRRLP  DH QP IENSFLDNHSSSRSESGNI
Sbjct: 181 LEKAVQNPSFVEHLARRVESMDFTAFNKKRRLPSGDHSQPAIENSFLDNHSSSRSESGNI 240

Query: 241 FHQDFSHKLRLESSTVSDVNLISCSTQSSNEEGGSSQRNMSKIDTRGVQENLHFATETLD 300
           FHQDFSHK+RLE+S+VSD+NL+SCSTQSSNE GGSSQRNMSK+DTRGVQE+ HFA ETLD
Sbjct: 241 FHQDFSHKMRLETSSVSDINLLSCSTQSSNEGGGSSQRNMSKLDTRGVQESHHFAAETLD 300

Query: 301 LSDTGTSFILKRDSSLSGKSPNDESPHLHSSQPSASSKEDGESHISCHLNLTLASSSLRV 360
           LSDTGTSF+LKRDSSLSGKSPNDESP LHS QP+ SSKEDGE+HISCHLNLTLASSSL+V
Sbjct: 301 LSDTGTSFVLKRDSSLSGKSPNDESPCLHSLQPNVSSKEDGENHISCHLNLTLASSSLQV 360

Query: 361 NDTACSVRMPQLGQTARKSPD---SNGKEADMRLFTKNINLDERTTPFCPQENLSNHHGP 420
           ND   SVRMPQLGQ  RKS     SNGKE+DMRLFTKNINLDE T+ FC +E  SN+HGP
Sbjct: 361 NDADYSVRMPQLGQNLRKSSGSKVSNGKESDMRLFTKNINLDEGTSTFCLKETSSNNHGP 420

Query: 421 SAAAAAAPVRANDFFWEQFLTERPGCPESEEASSNYRATLYKEPDDGRSGLSL 471
                A PVR ND FWEQFLTERPGCPESEEASSNYR  LYKEPDDGRSGLSL
Sbjct: 421 ----PANPVRVNDVFWEQFLTERPGCPESEEASSNYRENLYKEPDDGRSGLSL 469

BLAST of MS005643 vs. NCBI nr
Match: XP_022948442.1 (heat stress transcription factor A-5-like [Cucurbita moschata])

HSP 1 Score: 783.5 bits (2022), Expect = 1.0e-222
Identity = 406/474 (85.65%), Postives = 430/474 (90.72%), Query Frame = 0

Query: 1   MEAAAGGGGAGGAGPAPFLIKTYDMVDDSATDSIVSWTSSNKSFVVWNPPEFARLLLPTY 60
           MEAAAGGGG GGAGPAPFLIKTYDMVD+SATD IVSWTSS KSFVVWNPPEFARLLLPT+
Sbjct: 1   MEAAAGGGGTGGAGPAPFLIKTYDMVDESATDEIVSWTSSKKSFVVWNPPEFARLLLPTF 60

Query: 61  FKHNNFSSFIRQLNTYGFRKIDSERWEFANEDFIKDQKHLLKNIHRRKPIHSHSNPQGSL 120
           FKHNNFSSFIRQLNTYGF+KIDSERWEFAN+DFIKDQKHLLKNIHRRKPIHSHS P GSL
Sbjct: 61  FKHNNFSSFIRQLNTYGFKKIDSERWEFANDDFIKDQKHLLKNIHRRKPIHSHSTPPGSL 120

Query: 121 VDPERAAFEDEIERLSREKATLEANISRFKQQKSTAKHQVRDLTVKVESMEKRQKSLLAF 180
            DPERAAFEDEI+RLSR+K TLEA+ISRFKQQKSTAK Q++DLTVKVESMEKRQK+LLAF
Sbjct: 121 GDPERAAFEDEIDRLSRKKTTLEADISRFKQQKSTAKIQLQDLTVKVESMEKRQKNLLAF 180

Query: 181 LEKAVQNPSFVEHLARRVESMDFTAFNKKRRLPQADHPQPLIENSFLDNHSSSRSESGNI 240
           LEKAVQNPSFVEHLARRVESMDFTAFNKKRRLP ADH QP++ENSFLDNHSSSRS SGNI
Sbjct: 181 LEKAVQNPSFVEHLARRVESMDFTAFNKKRRLPSADHSQPVLENSFLDNHSSSRSGSGNI 240

Query: 241 FHQDFSHKLRLESSTVSDVNLISCSTQSSNEEGGSSQRNMSKIDTRGVQENLHFATETLD 300
           FHQDFSHK RLE+S+VSD+NL+SCSTQSSNE GGSSQRNMSK+DTRGVQE+ HFA ETLD
Sbjct: 241 FHQDFSHKTRLETSSVSDINLLSCSTQSSNEGGGSSQRNMSKLDTRGVQESHHFAAETLD 300

Query: 301 LSDTGTSFILKRDSSLSGKSPNDESPHLHSSQPSASSKEDGESHISCHLNLTLASSSLRV 360
           LSDTGTSF+LKRDSSLSGKSPNDESP LHS QP+ SSKEDGESHISCHLNLTLASSSLRV
Sbjct: 301 LSDTGTSFVLKRDSSLSGKSPNDESPCLHSLQPNVSSKEDGESHISCHLNLTLASSSLRV 360

Query: 361 NDTACSVRMPQLGQTARKSP----DSNGKEADMRLFTKNINLDERTTPFCPQENLSNHHG 420
           ND  CSVRMPQL Q  RKS     +SNGKE+DMRLFTKNINLDE TT  C +E  SN+HG
Sbjct: 361 NDADCSVRMPQLSQNLRKSSGSKVNSNGKESDMRLFTKNINLDEGTTTLCLKETSSNNHG 420

Query: 421 PSAAAAAAPVRANDFFWEQFLTERPGCPESEEASSNYRATLYKEPDDGRSGLSL 471
           P     A PVR ND FWEQFLTERPGCPESEEASSNYR  LYKEPDDGRSGLSL
Sbjct: 421 P----PANPVRVNDVFWEQFLTERPGCPESEEASSNYRENLYKEPDDGRSGLSL 470

BLAST of MS005643 vs. NCBI nr
Match: XP_038906704.1 (heat stress transcription factor A-5 [Benincasa hispida])

HSP 1 Score: 779.2 bits (2011), Expect = 1.9e-221
Identity = 409/474 (86.29%), Postives = 426/474 (89.87%), Query Frame = 0

Query: 1   MEAAAGGGGAGGAGPAPFLIKTYDMVDDSATDSIVSWTSSNKSFVVWNPPEFARLLLPTY 60
           MEAA GGG  GGAGPAPFLIKTYDMVDDS TD IVSWTSS KSFVVWNPPEFARLLLPT+
Sbjct: 1   MEAATGGGTTGGAGPAPFLIKTYDMVDDSTTDEIVSWTSSKKSFVVWNPPEFARLLLPTF 60

Query: 61  FKHNNFSSFIRQLNTYGFRKIDSERWEFANEDFIKDQKHLLKNIHRRKPIHSHSNPQGSL 120
           FKH+NFSSFIRQLNTYGFRKIDSE+WEFANEDFIKDQKHLLKNIHRRKPIHSHSNPQGS 
Sbjct: 61  FKHSNFSSFIRQLNTYGFRKIDSEKWEFANEDFIKDQKHLLKNIHRRKPIHSHSNPQGSH 120

Query: 121 VDPERAAFEDEIERLSREKATLEANISRFKQQKSTAKHQVRDLTVKVESMEKRQKSLLAF 180
           VDPERAAFEDEIERLSREK TLEANISRFKQQKSTAK Q++DLTVKVESMEKRQK+LLAF
Sbjct: 121 VDPERAAFEDEIERLSREKTTLEANISRFKQQKSTAKLQLQDLTVKVESMEKRQKNLLAF 180

Query: 181 LEKAVQNPSFVEHLARRVESMDFTAFNKKRRLPQADHPQPLIENSFLDNHSSSRSESGNI 240
           LEKAVQNPSFVEHLARRVESMDFTAF KKRRLP AD    +IENSFLDNHSSSRSESGNI
Sbjct: 181 LEKAVQNPSFVEHLARRVESMDFTAFKKKRRLPSADLSPLVIENSFLDNHSSSRSESGNI 240

Query: 241 FHQDFSHKLRLESSTVSDVNLISCSTQSSNEEGGSSQRNMSKIDTRGVQENLHFATETLD 300
           FHQDFSHKLRLE+S  SD+NLIS STQSSNEEGGSSQR+MSK+DTR VQENLHF  ETLD
Sbjct: 241 FHQDFSHKLRLETSCASDINLISRSTQSSNEEGGSSQRHMSKLDTRAVQENLHFVAETLD 300

Query: 301 LSDTGTSFILKRDSSLSGKSPNDESPHLHSSQPSASSKEDGESHISCHLNLTLASSSLRV 360
           LSDTGTSFILKRDSSLSGKSPNDESPHLHS QPS SSKEDGESHISC LNLTLASSSLR+
Sbjct: 301 LSDTGTSFILKRDSSLSGKSPNDESPHLHSLQPSVSSKEDGESHISCQLNLTLASSSLRI 360

Query: 361 NDTACSVRMPQLGQTARKSPD----SNGKEADMRLFTKNINLDERTTPFCPQENLSNHHG 420
           +DTACSVRMPQLGQ ARKSPD    SNGKE+D RLFTKNINLDERTT  CPQE  + ++G
Sbjct: 361 SDTACSVRMPQLGQNARKSPDSKVNSNGKESDARLFTKNINLDERTTLICPQETSNKNNG 420

Query: 421 PSAAAAAAPVRANDFFWEQFLTERPGCPESEEASSNYRATLYKEPDDGRSGLSL 471
           P          AND FWEQFLTERPGCPESEEASSNYRA LYKEP DGRSGLSL
Sbjct: 421 PP---------ANDGFWEQFLTERPGCPESEEASSNYRANLYKEPHDGRSGLSL 465

BLAST of MS005643 vs. NCBI nr
Match: XP_023539978.1 (heat stress transcription factor A-5-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 775.0 bits (2000), Expect = 3.7e-220
Identity = 402/474 (84.81%), Postives = 428/474 (90.30%), Query Frame = 0

Query: 1   MEAAAGGGGAGGAGPAPFLIKTYDMVDDSATDSIVSWTSSNKSFVVWNPPEFARLLLPTY 60
           ME AAGGGG GGAGPAPFLIKTYDMVD+SATD IVSWTSS KSFVVWNPPEFARLLLPT+
Sbjct: 1   MEGAAGGGGTGGAGPAPFLIKTYDMVDESATDKIVSWTSSKKSFVVWNPPEFARLLLPTF 60

Query: 61  FKHNNFSSFIRQLNTYGFRKIDSERWEFANEDFIKDQKHLLKNIHRRKPIHSHSNPQGSL 120
           FKHNNFSSFIRQLNTYGF+KIDSERWEFAN+DFIKDQKHLLKNIHRRKPIHSHS P GSL
Sbjct: 61  FKHNNFSSFIRQLNTYGFKKIDSERWEFANDDFIKDQKHLLKNIHRRKPIHSHSTPPGSL 120

Query: 121 VDPERAAFEDEIERLSREKATLEANISRFKQQKSTAKHQVRDLTVKVESMEKRQKSLLAF 180
            DPERAAFEDEI+RLSR+K TLEA+ISRFKQQKSTAK Q++DLTVKVESMEKRQK+LLAF
Sbjct: 121 GDPERAAFEDEIDRLSRKKTTLEADISRFKQQKSTAKTQLQDLTVKVESMEKRQKNLLAF 180

Query: 181 LEKAVQNPSFVEHLARRVESMDFTAFNKKRRLPQADHPQPLIENSFLDNHSSSRSESGNI 240
           LEKAVQNPSFVEHLARRVESMDFTAFNKKRRLP ADH QP++ENSFLDNHSSSRS SGNI
Sbjct: 181 LEKAVQNPSFVEHLARRVESMDFTAFNKKRRLPSADHSQPVLENSFLDNHSSSRSGSGNI 240

Query: 241 FHQDFSHKLRLESSTVSDVNLISCSTQSSNEEGGSSQRNMSKIDTRGVQENLHFATETLD 300
           FHQDFSHK+RLE+S+VSD+NL+SCSTQSSNE GGSSQRNMSK+DTRGVQE+ HFA ETLD
Sbjct: 241 FHQDFSHKMRLETSSVSDINLLSCSTQSSNEGGGSSQRNMSKLDTRGVQESHHFAAETLD 300

Query: 301 LSDTGTSFILKRDSSLSGKSPNDESPHLHSSQPSASSKEDGESHISCHLNLTLASSSLRV 360
           LSDTGTSF+LKRDSSLSGKSPNDESP LHS QP+ SSKEDGESHISCHLNLTLASSSLRV
Sbjct: 301 LSDTGTSFVLKRDSSLSGKSPNDESPCLHSLQPNVSSKEDGESHISCHLNLTLASSSLRV 360

Query: 361 NDTACSVRMPQLGQTARKSP----DSNGKEADMRLFTKNINLDERTTPFCPQENLSNHHG 420
           ND     RMPQLGQ  RKS     +SNGKE+DMRLFTKNINLDE TT  C +E  SN+HG
Sbjct: 361 NDADYLGRMPQLGQNLRKSSGSKVNSNGKESDMRLFTKNINLDEGTTTLCLKETSSNNHG 420

Query: 421 PSAAAAAAPVRANDFFWEQFLTERPGCPESEEASSNYRATLYKEPDDGRSGLSL 471
           P     A PVR ND FWEQFLTERPGCPESEEASSNYR  LYKEPD+GRSGLSL
Sbjct: 421 P----PANPVRVNDVFWEQFLTERPGCPESEEASSNYRENLYKEPDEGRSGLSL 470

BLAST of MS005643 vs. ExPASy Swiss-Prot
Match: Q94BZ5 (Heat stress transcription factor A-5 OS=Arabidopsis thaliana OX=3702 GN=HSFA5 PE=2 SV=1)

HSP 1 Score: 432.6 bits (1111), Expect = 5.8e-120
Identity = 249/470 (52.98%), Postives = 317/470 (67.45%), Query Frame = 0

Query: 3   AAAGGGGAGGAGPAPFLIKTYDMVDDSATDSIVSWTSSNKSFVVWNPPEFARLLLPTYFK 62
           +A+  GG G  GPAPFL+KTY+MVDDS+TD IVSW+++N SF+VWN  EF+RLLLPTYFK
Sbjct: 9   SASVSGGEGAGGPAPFLVKTYEMVDDSSTDQIVSWSANNNSFIVWNHAEFSRLLLPTYFK 68

Query: 63  HNNFSSFIRQLNTYGFRKIDSERWEFANEDFIKDQKHLLKNIHRRKPIHSHSNPQGSLVD 122
           HNNFSSFIRQLNTYGFRKID ERWEF N+DFIKDQKHLLKNIHRRKPIHSHS+P  S  D
Sbjct: 69  HNNFSSFIRQLNTYGFRKIDPERWEFLNDDFIKDQKHLLKNIHRRKPIHSHSHPPASSTD 128

Query: 123 PERAAFEDEIERLSREKATLEANISRFKQQKSTAKHQVRDLTVKVESMEKRQKSLLAFLE 182
            ERA  ++++++LSREKA +EA + +FKQQK  AKHQ  ++T  V+ ME RQK LL FLE
Sbjct: 129 QERAVLQEQMDKLSREKAAIEAKLLKFKQQKVVAKHQFEEMTEHVDDMENRQKKLLNFLE 188

Query: 183 KAVQNPSFVEHLARRVESMDFTAFNKKRRLPQADHPQPLIENSFLDNHS-SSRSESGNIF 242
            A++NP+FV++  ++VE +D +A+NKKRRLP+ +  +P  E+S LDN S SSR ESGNIF
Sbjct: 189 TAIRNPTFVKNFGKKVEQLDISAYNKKRRLPEVEQSKPPSEDSHLDNSSGSSRRESGNIF 248

Query: 243 HQDFSHKLRLESSTV-SDVNLISCSTQSSNEEGGSSQRNMSKID---TRGVQENLHFATE 302
           HQ+FS+KLRLE S   SD+N++S S QSSNEEG S +  +S  D   T   +E L FA E
Sbjct: 249 HQNFSNKLRLELSPADSDMNMVSHSIQSSNEEGASPKGILSGGDPNTTLTKREGLPFAPE 308

Query: 303 TLDLSDTGTSFILKRDSSLSGKSPNDESPHLHSSQPSASSKEDGESHISCHLNLTLASSS 362
            L+L+DTGT            +   +++  + + Q   +S E+ +   SCHLNLTLAS+ 
Sbjct: 309 ALELADTGT---------CPRRLLLNDNTRVETLQQRLTSSEETDGSFSCHLNLTLASAP 368

Query: 363 LRVNDTACSVRMPQLGQTARKSPDSNGKEADMRLFTKNINLDERTTPFCPQENLSNHHGP 422
           L   D   S    Q+ +T  KS + N    +     KN    E               G 
Sbjct: 369 L--PDKTAS----QIAKTTLKSQELNFNSIETSASEKNRGRQEIAV------------GG 428

Query: 423 SAAAAAAPVRANDFFWEQFLTERPGCPESEEASSNYRATLYKEPDDGRSG 468
           S A AA P R ND FWEQFLTERPG  ++EEASS YR   Y+E ++ R+G
Sbjct: 429 SQANAAPPARVNDVFWEQFLTERPGSSDNEEASSTYRGNPYEEQEEKRNG 451

BLAST of MS005643 vs. ExPASy Swiss-Prot
Match: Q6K6S5 (Heat stress transcription factor A-5 OS=Oryza sativa subsp. japonica OX=39947 GN=HSFA5 PE=2 SV=1)

HSP 1 Score: 364.8 bits (935), Expect = 1.5e-99
Identity = 240/487 (49.28%), Postives = 299/487 (61.40%), Query Frame = 0

Query: 3   AAAGGGGAGGAGPAPFLIKTYDMVDDSATDSIVSWT-SSNKSFVVWNPPEFARLLLPTYF 62
           A  GG G GG GPAPFL+KTY+MVDD +TD++VSW+ +S+ SFVVWN PEFA  LLP YF
Sbjct: 7   ARGGGAGGGGGGPAPFLLKTYEMVDDPSTDAVVSWSDASDASFVVWNHPEFAARLLPAYF 66

Query: 63  KHNNFSSFIRQLNTYGFRKIDSERWEFANEDFIKDQKHLLKNIHRRKPIHSHSNPQGSLV 122
           KH+NFSSFIRQLNTYGFRKID ERWEFANE FIK QKHLLKNIHRRKPIHSHS+P G+L 
Sbjct: 67  KHSNFSSFIRQLNTYGFRKIDPERWEFANEYFIKGQKHLLKNIHRRKPIHSHSHPPGALP 126

Query: 123 DPERAAFEDEIERLSREKATLEANISRFKQQKSTAKHQVRDLTVKVESMEKRQKSLLAFL 182
           D ERA FEDEIERLSREK+ L+A++ + KQQ+S   +Q+ DL  +V  ME+RQ  ++AFL
Sbjct: 127 DNERAIFEDEIERLSREKSNLQADLWKSKQQQSGTMNQIEDLERRVLGMEQRQTKMIAFL 186

Query: 183 EKAVQNPSFVEHLARRVE-SMDFT-AFNKKRRLPQADHPQPLIEN----SFLDNHSS-SR 242
           ++A +NP FV  L +  E S  FT AFNKKRRLP  D+    IEN    SF D+HSS S+
Sbjct: 187 QQASKNPQFVNKLVKMAEASSIFTDAFNKKRRLPGLDYS---IENTETTSFYDDHSSTSK 246

Query: 243 SESGNIFHQDFSHKLRLE-SSTVSDVNLISCSTQSSNEEGGSSQRNMSKIDTRGVQENLH 302
            E+GN+ +Q FS KLRL     +++ N+I+ STQSSNE+  S      + D  G +E L 
Sbjct: 247 QETGNLLNQHFSDKLRLGLCPAMTESNIITLSTQSSNEDNRSPHGKHPECDMMG-RECLP 306

Query: 303 FATETLDLSDTGTSFILKRDSSLSGKSPNDESPHLHSSQPSASSKEDGESHISCHLNLTL 362
              + ++LSDTGTS    + S  +               P  S     E  ++CHL+LTL
Sbjct: 307 LVPQMMELSDTGTSICPSKSSCFA---------------PPISD----EGLLTCHLSLTL 366

Query: 363 ASSSLRV------NDTACSVRMPQLGQTA---------RKSPDSNGKEADMRLFTKNINL 422
           AS S+ V      N    ++  P    TA         R   D+  K AD R        
Sbjct: 367 ASCSMDVDKSQGLNANGTTIDNPTEAATATMEKDDTIDRSFDDNQKKSADSR-------T 426

Query: 423 DERTTPFCPQENLSNHHGPSAAAAAAPVRANDFFWEQFLTERPGCPESEEASSNYRATLY 466
            + TTP       ++    S A AA     ND FWEQFLTERPGC E+EEASS  R    
Sbjct: 427 ADATTP------RADARVASEAPAAPAAVVNDKFWEQFLTERPGCSETEEASSGLRTDTS 457

BLAST of MS005643 vs. ExPASy Swiss-Prot
Match: O49403 (Heat stress transcription factor A-4a OS=Arabidopsis thaliana OX=3702 GN=HSFA4A PE=1 SV=1)

HSP 1 Score: 216.1 bits (549), Expect = 8.6e-55
Identity = 119/267 (44.57%), Postives = 170/267 (63.67%), Query Frame = 0

Query: 9   GAGGAGPAPFLIKTYDMVDDSATDSIVSWTSSNKSFVVWNPPEFARLLLPTYFKHNNFSS 68
           G   +   PFL KTY+MVDDS++DSIVSW+ SNKSF+VWNPPEF+R LLP +FKHNNFSS
Sbjct: 7   GVSSSSLPPFLTKTYEMVDDSSSDSIVSWSQSNKSFIVWNPPEFSRDLLPRFFKHNNFSS 66

Query: 69  FIRQLNTYGFRKIDSERWEFANEDFIKDQKHLLKNIHRRKPIHSHSNPQ-----GSLVDP 128
           FIRQLNTYGFRK D E+WEFAN+DF++ Q HL+KNIHRRKP+HSHS P        L D 
Sbjct: 67  FIRQLNTYGFRKADPEQWEFANDDFVRGQPHLMKNIHRRKPVHSHSLPNLQAQLNPLTDS 126

Query: 129 ERAAFEDEIERLSREKATLEANISRFKQQKSTAKHQVRDLTVKVESMEKRQKSLLAFLEK 188
           ER    ++IERL++EK  L   + +  +++   + QV++L  +++ MEKRQK++++F+ +
Sbjct: 127 ERVRMNNQIERLTKEKEGLLEELHKQDEEREVFEMQVKELKERLQHMEKRQKTMVSFVSQ 186

Query: 189 AVQNPSFVEHLARRVESMDFTAFNKKRRLPQADH--PQPLIENSFLDNHSSSRSESGNIF 248
            ++ P    +L+  V   +     +KRR P+ +    +P++E +           +    
Sbjct: 187 VLEKPGLALNLSPCVPETN----ERKRRFPRIEFFPDEPMLEENKTCVVVREEGSTSPSS 246

Query: 249 HQDFSHKLRLESSTVSDVNLISCSTQS 269
           H       +LESS     NL+S S +S
Sbjct: 247 HTREHQVEQLESSIAIWENLVSDSCES 269

BLAST of MS005643 vs. ExPASy Swiss-Prot
Match: Q84T61 (Heat stress transcription factor A-1 OS=Oryza sativa subsp. japonica OX=39947 GN=HSFA1 PE=2 SV=1)

HSP 1 Score: 213.8 bits (543), Expect = 4.3e-54
Identity = 113/225 (50.22%), Postives = 151/225 (67.11%), Query Frame = 0

Query: 3   AAAGGGGAGGAGPAPFLIKTYDMVDDSATDSIVSWTSSNKSFVVWNPPEFARLLLPTYFK 62
           AA   G A    P PFL+KTY+MVDD ATD++VSW   N SFVVWN PEFAR LLP YFK
Sbjct: 25  AAVSNGVA--TAPPPFLMKTYEMVDDPATDAVVSWGPGNNSFVVWNTPEFARDLLPKYFK 84

Query: 63  HNNFSSFIRQLNTYGFRKIDSERWEFANEDFIKDQKHLLKNIHRRKPIHSHSN------- 122
           H+NFSSF+RQLNTYGFRK+D +RWEFANE F++ QKHLLK I+RRKP H ++        
Sbjct: 85  HSNFSSFVRQLNTYGFRKVDPDRWEFANEGFLRGQKHLLKTINRRKPTHGNNQVQQPQLP 144

Query: 123 --PQGSLVDPERAAFEDEIERLSREKATLEANISRFKQQKSTAKHQVRDLTVKVESMEKR 182
             P  + V+  +   E+EIE L R+K  L   + R +QQ+ T  HQ++ L  +++ ME+R
Sbjct: 145 AAPVPACVEVGKFGMEEEIEMLKRDKNVLMQELVRLRQQQQTTDHQLQTLGKRLQGMEQR 204

Query: 183 QKSLLAFLEKAVQNPSFVEHLARRVES--MDFTAFNKKRRLPQAD 217
           Q+ +++FL KA+ +P F+    ++ E+      A NKKRRLP+ D
Sbjct: 205 QQQMMSFLAKAMHSPGFLAQFVQQNENSRRRIVASNKKRRLPKQD 247

BLAST of MS005643 vs. ExPASy Swiss-Prot
Match: P41151 (Heat stress transcription factor A-1a OS=Arabidopsis thaliana OX=3702 GN=HSFA1A PE=1 SV=2)

HSP 1 Score: 211.8 bits (538), Expect = 1.6e-53
Identity = 109/218 (50.00%), Postives = 148/218 (67.89%), Query Frame = 0

Query: 15  PAPFLIKTYDMVDDSATDSIVSWTSSNKSFVVWNPPEFARLLLPTYFKHNNFSSFIRQLN 74
           P PFL KTYDMV+D ATD+IVSW+ +N SF+VW+PPEF+R LLP YFKHNNFSSF+RQLN
Sbjct: 50  PPPFLSKTYDMVEDPATDAIVSWSPTNNSFIVWDPPEFSRDLLPKYFKHNNFSSFVRQLN 109

Query: 75  TYGFRKIDSERWEFANEDFIKDQKHLLKNIHRRKPIHSH----SNPQ------------- 134
           TYGFRK+D +RWEFANE F++ QKHLLK I RRK +  H    SNPQ             
Sbjct: 110 TYGFRKVDPDRWEFANEGFLRGQKHLLKKISRRKSVQGHGSSSSNPQSQQLSQGQGSMAA 169

Query: 135 -GSLVDPERAAFEDEIERLSREKATLEANISRFKQQKSTAKHQVRDLTVKVESMEKRQKS 194
             S V+  +   E+E+E+L R+K  L   + + +QQ+ T  ++++ L   ++ ME+RQ+ 
Sbjct: 170 LSSCVEVGKFGLEEEVEQLKRDKNVLMQELVKLRQQQQTTDNKLQVLVKHLQVMEQRQQQ 229

Query: 195 LLAFLEKAVQNPSFVEHLARRV--ESMDFTAFNKKRRL 213
           +++FL KAVQNP+F+    ++    +M  T  NKKRRL
Sbjct: 230 IMSFLAKAVQNPTFLSQFIQKQTDSNMHVTEANKKRRL 267

BLAST of MS005643 vs. ExPASy TrEMBL
Match: A0A6J1CYL3 (heat stress transcription factor A-5 OS=Momordica charantia OX=3673 GN=LOC111015981 PE=3 SV=1)

HSP 1 Score: 906.0 bits (2340), Expect = 6.7e-260
Identity = 467/470 (99.36%), Postives = 467/470 (99.36%), Query Frame = 0

Query: 1   MEAAAGGGGAGGAGPAPFLIKTYDMVDDSATDSIVSWTSSNKSFVVWNPPEFARLLLPTY 60
           MEAAAGGGGAGGAGPAPFLIKTYDMVDDSATDSIVSWTSSNKSFVVWNPPEFARLLLPTY
Sbjct: 1   MEAAAGGGGAGGAGPAPFLIKTYDMVDDSATDSIVSWTSSNKSFVVWNPPEFARLLLPTY 60

Query: 61  FKHNNFSSFIRQLNTYGFRKIDSERWEFANEDFIKDQKHLLKNIHRRKPIHSHSNPQGSL 120
           FKHNNFSSFIRQLNTYGFRKIDSERWEFANEDFIKDQKHLLKNIHRRKPIHSHSNPQGSL
Sbjct: 61  FKHNNFSSFIRQLNTYGFRKIDSERWEFANEDFIKDQKHLLKNIHRRKPIHSHSNPQGSL 120

Query: 121 VDPERAAFEDEIERLSREKATLEANISRFKQQKSTAKHQVRDLTVKVESMEKRQKSLLAF 180
           VDPERAAFEDEIERLSREKATLEANISRFKQQKSTAKHQVRDLTVKVESMEKRQKSLLAF
Sbjct: 121 VDPERAAFEDEIERLSREKATLEANISRFKQQKSTAKHQVRDLTVKVESMEKRQKSLLAF 180

Query: 181 LEKAVQNPSFVEHLARRVESMDFTAFNKKRRLPQADHPQPLIENSFLDNHSSSRSESGNI 240
           LEKAVQNPSFVEHLARRVESMDFTAFNKKRRLPQADHPQPLIENSFLDNHSSSRSESGNI
Sbjct: 181 LEKAVQNPSFVEHLARRVESMDFTAFNKKRRLPQADHPQPLIENSFLDNHSSSRSESGNI 240

Query: 241 FHQDFSHKLRLESSTVSDVNLISCSTQSSNEEGGSSQRNMSKIDTRGVQENLHFATETLD 300
           FHQDFSHKLRLESSTVSDVNLISCSTQSSNEEGGSSQRNMSKIDTRGVQENLHFATETLD
Sbjct: 241 FHQDFSHKLRLESSTVSDVNLISCSTQSSNEEGGSSQRNMSKIDTRGVQENLHFATETLD 300

Query: 301 LSDTGTSFILKRDSSLSGKSPNDESPHLHSSQPSASSKEDGESHISCHLNLTLASSSLRV 360
           LSDTGTSFILKRDSSLSGKSPNDESP L  SQPSASSKEDGESHISCHLNLTLASSSLRV
Sbjct: 301 LSDTGTSFILKRDSSLSGKSPNDESPRL--SQPSASSKEDGESHISCHLNLTLASSSLRV 360

Query: 361 NDTACSVRMPQLGQTARKSPDSNGKEADMRLFTKNINLDERTTPFCPQENLSNHHGPSAA 420
           NDTACSVRMPQLGQTARKSPDSNGKEADMRLFTKNINLDERTTPFCPQENLSNHHGPSAA
Sbjct: 361 NDTACSVRMPQLGQTARKSPDSNGKEADMRLFTKNINLDERTTPFCPQENLSNHHGPSAA 420

Query: 421 AAAAPVRANDFFWEQFLTERPGCPESEEASSNYRATLYKEPDDGRSGLSL 471
           AAAAPVRANDFFWEQFLTERPGCPESEEASSNYRATLYKEPDDGRSGLSL
Sbjct: 421 AAAAPVRANDFFWEQFLTERPGCPESEEASSNYRATLYKEPDDGRSGLSL 468

BLAST of MS005643 vs. ExPASy TrEMBL
Match: A0A6J1IB49 (heat stress transcription factor A-5-like OS=Cucurbita maxima OX=3661 GN=LOC111473483 PE=3 SV=1)

HSP 1 Score: 784.3 bits (2024), Expect = 2.9e-223
Identity = 406/473 (85.84%), Postives = 431/473 (91.12%), Query Frame = 0

Query: 1   MEAAAGGGGAGGAGPAPFLIKTYDMVDDSATDSIVSWTSSNKSFVVWNPPEFARLLLPTY 60
           MEAAAGGGG GGAGPAPFLIKTYDMVD+SATD IVSWTSS KSFVVWNPPEFARLLLPT+
Sbjct: 1   MEAAAGGGGTGGAGPAPFLIKTYDMVDESATDEIVSWTSSKKSFVVWNPPEFARLLLPTF 60

Query: 61  FKHNNFSSFIRQLNTYGFRKIDSERWEFANEDFIKDQKHLLKNIHRRKPIHSHSNPQGSL 120
           FKHNNFSSFIRQLNTYGF+KIDSERWEFAN+DFIKDQKHLLKNIHRRKPIHSHS P GSL
Sbjct: 61  FKHNNFSSFIRQLNTYGFKKIDSERWEFANDDFIKDQKHLLKNIHRRKPIHSHSTPPGSL 120

Query: 121 VDPERAAFEDEIERLSREKATLEANISRFKQQKSTAKHQVRDLTVKVESMEKRQKSLLAF 180
           VDPERAAFEDEI+RLSR+K TLEA+ISRFKQQKSTAK Q++DLTVKVESMEKRQK+LLAF
Sbjct: 121 VDPERAAFEDEIDRLSRKKTTLEADISRFKQQKSTAKIQLQDLTVKVESMEKRQKNLLAF 180

Query: 181 LEKAVQNPSFVEHLARRVESMDFTAFNKKRRLPQADHPQPLIENSFLDNHSSSRSESGNI 240
           LEKAVQNPSFVEHLARRVESMDFTAFNKKRRLP  DH QP IENSFLDNHSSSRSESGNI
Sbjct: 181 LEKAVQNPSFVEHLARRVESMDFTAFNKKRRLPSGDHSQPAIENSFLDNHSSSRSESGNI 240

Query: 241 FHQDFSHKLRLESSTVSDVNLISCSTQSSNEEGGSSQRNMSKIDTRGVQENLHFATETLD 300
           FHQDFSHK+RLE+S+VSD+NL+SCSTQSSNE GGSSQRNMSK+DTRGVQE+ HFA ETLD
Sbjct: 241 FHQDFSHKMRLETSSVSDINLLSCSTQSSNEGGGSSQRNMSKLDTRGVQESHHFAAETLD 300

Query: 301 LSDTGTSFILKRDSSLSGKSPNDESPHLHSSQPSASSKEDGESHISCHLNLTLASSSLRV 360
           LSDTGTSF+LKRDSSLSGKSPNDESP LHS QP+ SSKEDGE+HISCHLNLTLASSSL+V
Sbjct: 301 LSDTGTSFVLKRDSSLSGKSPNDESPCLHSLQPNVSSKEDGENHISCHLNLTLASSSLQV 360

Query: 361 NDTACSVRMPQLGQTARKSPD---SNGKEADMRLFTKNINLDERTTPFCPQENLSNHHGP 420
           ND   SVRMPQLGQ  RKS     SNGKE+DMRLFTKNINLDE T+ FC +E  SN+HGP
Sbjct: 361 NDADYSVRMPQLGQNLRKSSGSKVSNGKESDMRLFTKNINLDEGTSTFCLKETSSNNHGP 420

Query: 421 SAAAAAAPVRANDFFWEQFLTERPGCPESEEASSNYRATLYKEPDDGRSGLSL 471
                A PVR ND FWEQFLTERPGCPESEEASSNYR  LYKEPDDGRSGLSL
Sbjct: 421 ----PANPVRVNDVFWEQFLTERPGCPESEEASSNYRENLYKEPDDGRSGLSL 469

BLAST of MS005643 vs. ExPASy TrEMBL
Match: A0A6J1G983 (heat stress transcription factor A-5-like OS=Cucurbita moschata OX=3662 GN=LOC111452123 PE=3 SV=1)

HSP 1 Score: 783.5 bits (2022), Expect = 5.0e-223
Identity = 406/474 (85.65%), Postives = 430/474 (90.72%), Query Frame = 0

Query: 1   MEAAAGGGGAGGAGPAPFLIKTYDMVDDSATDSIVSWTSSNKSFVVWNPPEFARLLLPTY 60
           MEAAAGGGG GGAGPAPFLIKTYDMVD+SATD IVSWTSS KSFVVWNPPEFARLLLPT+
Sbjct: 1   MEAAAGGGGTGGAGPAPFLIKTYDMVDESATDEIVSWTSSKKSFVVWNPPEFARLLLPTF 60

Query: 61  FKHNNFSSFIRQLNTYGFRKIDSERWEFANEDFIKDQKHLLKNIHRRKPIHSHSNPQGSL 120
           FKHNNFSSFIRQLNTYGF+KIDSERWEFAN+DFIKDQKHLLKNIHRRKPIHSHS P GSL
Sbjct: 61  FKHNNFSSFIRQLNTYGFKKIDSERWEFANDDFIKDQKHLLKNIHRRKPIHSHSTPPGSL 120

Query: 121 VDPERAAFEDEIERLSREKATLEANISRFKQQKSTAKHQVRDLTVKVESMEKRQKSLLAF 180
            DPERAAFEDEI+RLSR+K TLEA+ISRFKQQKSTAK Q++DLTVKVESMEKRQK+LLAF
Sbjct: 121 GDPERAAFEDEIDRLSRKKTTLEADISRFKQQKSTAKIQLQDLTVKVESMEKRQKNLLAF 180

Query: 181 LEKAVQNPSFVEHLARRVESMDFTAFNKKRRLPQADHPQPLIENSFLDNHSSSRSESGNI 240
           LEKAVQNPSFVEHLARRVESMDFTAFNKKRRLP ADH QP++ENSFLDNHSSSRS SGNI
Sbjct: 181 LEKAVQNPSFVEHLARRVESMDFTAFNKKRRLPSADHSQPVLENSFLDNHSSSRSGSGNI 240

Query: 241 FHQDFSHKLRLESSTVSDVNLISCSTQSSNEEGGSSQRNMSKIDTRGVQENLHFATETLD 300
           FHQDFSHK RLE+S+VSD+NL+SCSTQSSNE GGSSQRNMSK+DTRGVQE+ HFA ETLD
Sbjct: 241 FHQDFSHKTRLETSSVSDINLLSCSTQSSNEGGGSSQRNMSKLDTRGVQESHHFAAETLD 300

Query: 301 LSDTGTSFILKRDSSLSGKSPNDESPHLHSSQPSASSKEDGESHISCHLNLTLASSSLRV 360
           LSDTGTSF+LKRDSSLSGKSPNDESP LHS QP+ SSKEDGESHISCHLNLTLASSSLRV
Sbjct: 301 LSDTGTSFVLKRDSSLSGKSPNDESPCLHSLQPNVSSKEDGESHISCHLNLTLASSSLRV 360

Query: 361 NDTACSVRMPQLGQTARKSP----DSNGKEADMRLFTKNINLDERTTPFCPQENLSNHHG 420
           ND  CSVRMPQL Q  RKS     +SNGKE+DMRLFTKNINLDE TT  C +E  SN+HG
Sbjct: 361 NDADCSVRMPQLSQNLRKSSGSKVNSNGKESDMRLFTKNINLDEGTTTLCLKETSSNNHG 420

Query: 421 PSAAAAAAPVRANDFFWEQFLTERPGCPESEEASSNYRATLYKEPDDGRSGLSL 471
           P     A PVR ND FWEQFLTERPGCPESEEASSNYR  LYKEPDDGRSGLSL
Sbjct: 421 P----PANPVRVNDVFWEQFLTERPGCPESEEASSNYRENLYKEPDDGRSGLSL 470

BLAST of MS005643 vs. ExPASy TrEMBL
Match: A0A1S3AWR2 (heat stress transcription factor A-5 OS=Cucumis melo OX=3656 GN=LOC103483459 PE=3 SV=1)

HSP 1 Score: 771.2 bits (1990), Expect = 2.6e-219
Identity = 399/468 (85.26%), Postives = 424/468 (90.60%), Query Frame = 0

Query: 1   MEAAAGGGGAGGAGPAPFLIKTYDMVDDSATDSIVSWTSSNKSFVVWNPPEFARLLLPTY 60
           MEAAAGGGG GGAGPAPFL+KTYDMVDDS+TD IVSWTSS KSFVVWNPPEFARLLLPT+
Sbjct: 1   MEAAAGGGGTGGAGPAPFLLKTYDMVDDSSTDEIVSWTSSKKSFVVWNPPEFARLLLPTF 60

Query: 61  FKHNNFSSFIRQLNTYGFRKIDSERWEFANEDFIKDQKHLLKNIHRRKPIHSHSNPQGSL 120
           FKH+NFSSFIRQLNTYGFRKIDSE+WEFANEDFIKDQKHLLKNIHRRKPIHSHSNPQGS 
Sbjct: 61  FKHSNFSSFIRQLNTYGFRKIDSEKWEFANEDFIKDQKHLLKNIHRRKPIHSHSNPQGSH 120

Query: 121 VDPERAAFEDEIERLSREKATLEANISRFKQQKSTAKHQVRDLTVKVESMEKRQKSLLAF 180
           +DPERAAFEDEIERLSREK TLE NISRFKQQKSTAK Q++DLTVKVESMEKRQK+LLAF
Sbjct: 121 IDPERAAFEDEIERLSREKNTLEVNISRFKQQKSTAKLQLQDLTVKVESMEKRQKNLLAF 180

Query: 181 LEKAVQNPSFVEHLARRVESMDFTAFNKKRRLPQADHPQPLIENSFLDNHSSSRSESGNI 240
           LEKAVQNPSFVEHLARRVESMDFTAF KKRRLP AD  QP+IENSFLDNHSSSRSESGNI
Sbjct: 181 LEKAVQNPSFVEHLARRVESMDFTAFKKKRRLPSADLSQPVIENSFLDNHSSSRSESGNI 240

Query: 241 FHQDFSHKLRLESSTVSDVNLISCSTQSSNEEGGSSQRNMSKIDTRGVQENLHFATETLD 300
           FHQDFS KLRLE+S  SD+NLIS STQSSNEEGGSSQR +SK DTR VQENLHFA ETLD
Sbjct: 241 FHQDFSQKLRLETSCASDINLISRSTQSSNEEGGSSQRQLSKFDTRAVQENLHFAAETLD 300

Query: 301 LSDTGTSFILKRDSSLSGKSPNDESPHLHSSQPSASSKEDGESHISCHLNLTLASSSLRV 360
           LSDTGTSFIL+RDSSLSGKS ND++P LHS QPS SSKEDGESHISC LNLTLASSSLR+
Sbjct: 301 LSDTGTSFILRRDSSLSGKSHNDDNPRLHSLQPSVSSKEDGESHISCQLNLTLASSSLRI 360

Query: 361 NDTACSVRMPQLGQTARKSPD----SNGKEADMRLFTKNINLDERTTPFCPQENLSNHHG 420
           NDTACSVRMPQLGQ  RK PD    SNGKE+D+RLFTKNINLDE +TP CPQE  +N+HG
Sbjct: 361 NDTACSVRMPQLGQNVRKFPDSKANSNGKESDVRLFTKNINLDEGSTPVCPQETSNNNHG 420

Query: 421 PSAAAAAAPVRANDFFWEQFLTERPGCPESEEASSNYRATLYKEPDDG 465
           P AA+    +RAND FWE+ LTERPGCPESEEASSNYRA  YKEPDDG
Sbjct: 421 PPAAS----IRANDVFWERLLTERPGCPESEEASSNYRANPYKEPDDG 464

BLAST of MS005643 vs. ExPASy TrEMBL
Match: A0A0A0L9B5 (HSF_DOMAIN domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G129630 PE=3 SV=1)

HSP 1 Score: 766.5 bits (1978), Expect = 6.3e-218
Identity = 396/468 (84.62%), Postives = 423/468 (90.38%), Query Frame = 0

Query: 1   MEAAAGGGGAGGAGPAPFLIKTYDMVDDSATDSIVSWTSSNKSFVVWNPPEFARLLLPTY 60
           MEAAA GGG GGAGPAPFLIKTYDMVDDS+TD IVSWTSS KSFVVWNPPEFARLLLPT+
Sbjct: 87  MEAAAAGGGTGGAGPAPFLIKTYDMVDDSSTDEIVSWTSSKKSFVVWNPPEFARLLLPTF 146

Query: 61  FKHNNFSSFIRQLNTYGFRKIDSERWEFANEDFIKDQKHLLKNIHRRKPIHSHSNPQGSL 120
           FKH+NFSSFIRQLNTYGFRKIDSE+WEFANEDFIKDQKHLLKNIHRRKPIHSHSNPQGS 
Sbjct: 147 FKHSNFSSFIRQLNTYGFRKIDSEKWEFANEDFIKDQKHLLKNIHRRKPIHSHSNPQGSH 206

Query: 121 VDPERAAFEDEIERLSREKATLEANISRFKQQKSTAKHQVRDLTVKVESMEKRQKSLLAF 180
           +DPERAAFEDEIERL+REK TLE NISRFKQQKSTAK Q++DLTVKVESMEKRQK+LLAF
Sbjct: 207 IDPERAAFEDEIERLAREKTTLETNISRFKQQKSTAKLQLQDLTVKVESMEKRQKNLLAF 266

Query: 181 LEKAVQNPSFVEHLARRVESMDFTAFNKKRRLPQADHPQPLIENSFLDNHSSSRSESGNI 240
           LEKAVQNPSFVEHLARRVESMDFTAF KKRRLP AD  QP++ENSFLDNHSSSRSESGNI
Sbjct: 267 LEKAVQNPSFVEHLARRVESMDFTAFKKKRRLPSADLSQPVVENSFLDNHSSSRSESGNI 326

Query: 241 FHQDFSHKLRLESSTVSDVNLISCSTQSSNEEGGSSQRNMSKIDTRGVQENLHFATETLD 300
           FHQDFS KLRLE+S  SD+NLIS STQSSNEEGGSSQR +SK DTR VQEN+HFA ETLD
Sbjct: 327 FHQDFSQKLRLETSCASDINLISRSTQSSNEEGGSSQRQLSKFDTRAVQENIHFAVETLD 386

Query: 301 LSDTGTSFILKRDSSLSGKSPNDESPHLHSSQPSASSKEDGESHISCHLNLTLASSSLRV 360
           LSDTGTSFIL+RDSSLSGKS ND+SP LHS QPS SSKEDGESHISC LNLTLASSSLR+
Sbjct: 387 LSDTGTSFILRRDSSLSGKSHNDDSPCLHSLQPSVSSKEDGESHISCQLNLTLASSSLRI 446

Query: 361 NDTACSVRMPQLGQTARKSPD----SNGKEADMRLFTKNINLDERTTPFCPQENLSNHHG 420
           NDTACSVRMPQLGQ  RK PD    SNGKE+D+RLFTKNINLDE +TP CPQE  +N+HG
Sbjct: 447 NDTACSVRMPQLGQNVRKFPDSKVNSNGKESDVRLFTKNINLDEGSTPVCPQETSNNNHG 506

Query: 421 PSAAAAAAPVRANDFFWEQFLTERPGCPESEEASSNYRATLYKEPDDG 465
           P AA+    +RAND FWE+ LTERPGCPESEEASSNYRA  +KEPDDG
Sbjct: 507 PPAAS----IRANDVFWERLLTERPGCPESEEASSNYRANPFKEPDDG 550

BLAST of MS005643 vs. TAIR 10
Match: AT4G13980.1 (winged-helix DNA-binding transcription factor family protein )

HSP 1 Score: 432.6 bits (1111), Expect = 4.1e-121
Identity = 249/470 (52.98%), Postives = 317/470 (67.45%), Query Frame = 0

Query: 3   AAAGGGGAGGAGPAPFLIKTYDMVDDSATDSIVSWTSSNKSFVVWNPPEFARLLLPTYFK 62
           +A+  GG G  GPAPFL+KTY+MVDDS+TD IVSW+++N SF+VWN  EF+RLLLPTYFK
Sbjct: 9   SASVSGGEGAGGPAPFLVKTYEMVDDSSTDQIVSWSANNNSFIVWNHAEFSRLLLPTYFK 68

Query: 63  HNNFSSFIRQLNTYGFRKIDSERWEFANEDFIKDQKHLLKNIHRRKPIHSHSNPQGSLVD 122
           HNNFSSFIRQLNTYGFRKID ERWEF N+DFIKDQKHLLKNIHRRKPIHSHS+P  S  D
Sbjct: 69  HNNFSSFIRQLNTYGFRKIDPERWEFLNDDFIKDQKHLLKNIHRRKPIHSHSHPPASSTD 128

Query: 123 PERAAFEDEIERLSREKATLEANISRFKQQKSTAKHQVRDLTVKVESMEKRQKSLLAFLE 182
            ERA  ++++++LSREKA +EA + +FKQQK  AKHQ  ++T  V+ ME RQK LL FLE
Sbjct: 129 QERAVLQEQMDKLSREKAAIEAKLLKFKQQKVVAKHQFEEMTEHVDDMENRQKKLLNFLE 188

Query: 183 KAVQNPSFVEHLARRVESMDFTAFNKKRRLPQADHPQPLIENSFLDNHS-SSRSESGNIF 242
            A++NP+FV++  ++VE +D +A+NKKRRLP+ +  +P  E+S LDN S SSR ESGNIF
Sbjct: 189 TAIRNPTFVKNFGKKVEQLDISAYNKKRRLPEVEQSKPPSEDSHLDNSSGSSRRESGNIF 248

Query: 243 HQDFSHKLRLESSTV-SDVNLISCSTQSSNEEGGSSQRNMSKID---TRGVQENLHFATE 302
           HQ+FS+KLRLE S   SD+N++S S QSSNEEG S +  +S  D   T   +E L FA E
Sbjct: 249 HQNFSNKLRLELSPADSDMNMVSHSIQSSNEEGASPKGILSGGDPNTTLTKREGLPFAPE 308

Query: 303 TLDLSDTGTSFILKRDSSLSGKSPNDESPHLHSSQPSASSKEDGESHISCHLNLTLASSS 362
            L+L+DTGT            +   +++  + + Q   +S E+ +   SCHLNLTLAS+ 
Sbjct: 309 ALELADTGT---------CPRRLLLNDNTRVETLQQRLTSSEETDGSFSCHLNLTLASAP 368

Query: 363 LRVNDTACSVRMPQLGQTARKSPDSNGKEADMRLFTKNINLDERTTPFCPQENLSNHHGP 422
           L   D   S    Q+ +T  KS + N    +     KN    E               G 
Sbjct: 369 L--PDKTAS----QIAKTTLKSQELNFNSIETSASEKNRGRQEIAV------------GG 428

Query: 423 SAAAAAAPVRANDFFWEQFLTERPGCPESEEASSNYRATLYKEPDDGRSG 468
           S A AA P R ND FWEQFLTERPG  ++EEASS YR   Y+E ++ R+G
Sbjct: 429 SQANAAPPARVNDVFWEQFLTERPGSSDNEEASSTYRGNPYEEQEEKRNG 451

BLAST of MS005643 vs. TAIR 10
Match: AT4G18880.1 (heat shock transcription factor A4A )

HSP 1 Score: 216.1 bits (549), Expect = 6.1e-56
Identity = 119/267 (44.57%), Postives = 170/267 (63.67%), Query Frame = 0

Query: 9   GAGGAGPAPFLIKTYDMVDDSATDSIVSWTSSNKSFVVWNPPEFARLLLPTYFKHNNFSS 68
           G   +   PFL KTY+MVDDS++DSIVSW+ SNKSF+VWNPPEF+R LLP +FKHNNFSS
Sbjct: 7   GVSSSSLPPFLTKTYEMVDDSSSDSIVSWSQSNKSFIVWNPPEFSRDLLPRFFKHNNFSS 66

Query: 69  FIRQLNTYGFRKIDSERWEFANEDFIKDQKHLLKNIHRRKPIHSHSNPQ-----GSLVDP 128
           FIRQLNTYGFRK D E+WEFAN+DF++ Q HL+KNIHRRKP+HSHS P        L D 
Sbjct: 67  FIRQLNTYGFRKADPEQWEFANDDFVRGQPHLMKNIHRRKPVHSHSLPNLQAQLNPLTDS 126

Query: 129 ERAAFEDEIERLSREKATLEANISRFKQQKSTAKHQVRDLTVKVESMEKRQKSLLAFLEK 188
           ER    ++IERL++EK  L   + +  +++   + QV++L  +++ MEKRQK++++F+ +
Sbjct: 127 ERVRMNNQIERLTKEKEGLLEELHKQDEEREVFEMQVKELKERLQHMEKRQKTMVSFVSQ 186

Query: 189 AVQNPSFVEHLARRVESMDFTAFNKKRRLPQADH--PQPLIENSFLDNHSSSRSESGNIF 248
            ++ P    +L+  V   +     +KRR P+ +    +P++E +           +    
Sbjct: 187 VLEKPGLALNLSPCVPETN----ERKRRFPRIEFFPDEPMLEENKTCVVVREEGSTSPSS 246

Query: 249 HQDFSHKLRLESSTVSDVNLISCSTQS 269
           H       +LESS     NL+S S +S
Sbjct: 247 HTREHQVEQLESSIAIWENLVSDSCES 269

BLAST of MS005643 vs. TAIR 10
Match: AT4G17750.1 (heat shock factor 1 )

HSP 1 Score: 211.8 bits (538), Expect = 1.2e-54
Identity = 109/218 (50.00%), Postives = 148/218 (67.89%), Query Frame = 0

Query: 15  PAPFLIKTYDMVDDSATDSIVSWTSSNKSFVVWNPPEFARLLLPTYFKHNNFSSFIRQLN 74
           P PFL KTYDMV+D ATD+IVSW+ +N SF+VW+PPEF+R LLP YFKHNNFSSF+RQLN
Sbjct: 50  PPPFLSKTYDMVEDPATDAIVSWSPTNNSFIVWDPPEFSRDLLPKYFKHNNFSSFVRQLN 109

Query: 75  TYGFRKIDSERWEFANEDFIKDQKHLLKNIHRRKPIHSH----SNPQ------------- 134
           TYGFRK+D +RWEFANE F++ QKHLLK I RRK +  H    SNPQ             
Sbjct: 110 TYGFRKVDPDRWEFANEGFLRGQKHLLKKISRRKSVQGHGSSSSNPQSQQLSQGQGSMAA 169

Query: 135 -GSLVDPERAAFEDEIERLSREKATLEANISRFKQQKSTAKHQVRDLTVKVESMEKRQKS 194
             S V+  +   E+E+E+L R+K  L   + + +QQ+ T  ++++ L   ++ ME+RQ+ 
Sbjct: 170 LSSCVEVGKFGLEEEVEQLKRDKNVLMQELVKLRQQQQTTDNKLQVLVKHLQVMEQRQQQ 229

Query: 195 LLAFLEKAVQNPSFVEHLARRV--ESMDFTAFNKKRRL 213
           +++FL KAVQNP+F+    ++    +M  T  NKKRRL
Sbjct: 230 IMSFLAKAVQNPTFLSQFIQKQTDSNMHVTEANKKRRL 267

BLAST of MS005643 vs. TAIR 10
Match: AT5G16820.1 (heat shock factor 3 )

HSP 1 Score: 209.5 bits (532), Expect = 5.7e-54
Identity = 105/209 (50.24%), Postives = 145/209 (69.38%), Query Frame = 0

Query: 17  PFLIKTYDMVDDSATDSIVSWTSSNKSFVVWNPPEFARLLLPTYFKHNNFSSFIRQLNTY 76
           PFL KTYDMVDD  T+ +VSW+S N SFVVW+ PEF+++LLP YFKHNNFSSF+RQLNTY
Sbjct: 27  PFLSKTYDMVDDPLTNEVVSWSSGNNSFVVWSAPEFSKVLLPKYFKHNNFSSFVRQLNTY 86

Query: 77  GFRKIDSERWEFANEDFIKDQKHLLKNIHRRKPIHSHSNPQ---------GSLVDPERAA 136
           GFRK+D +RWEFANE F++ +K LLK+I RRKP H   N Q         G+ V+  +  
Sbjct: 87  GFRKVDPDRWEFANEGFLRGRKQLLKSIVRRKPSHVQQNQQQTQVQSSSVGACVEVGKFG 146

Query: 137 FEDEIERLSREKATLEANISRFKQQKSTAKHQVRDLTVKVESMEKRQKSLLAFLEKAVQN 196
            E+E+ERL R+K  L   + R +QQ+   ++Q++++  KV+ ME+RQ+ +++FL KAVQ+
Sbjct: 147 IEEEVERLKRDKNVLMQELVRLRQQQQATENQLQNVGQKVQVMEQRQQQMMSFLAKAVQS 206

Query: 197 PSFVEHLARRVE---SMDFTAFNKKRRLP 214
           P F+  L ++     +      NKKRRLP
Sbjct: 207 PGFLNQLVQQNNNDGNRQIPGSNKKRRLP 235

BLAST of MS005643 vs. TAIR 10
Match: AT5G16820.2 (heat shock factor 3 )

HSP 1 Score: 209.5 bits (532), Expect = 5.7e-54
Identity = 105/209 (50.24%), Postives = 145/209 (69.38%), Query Frame = 0

Query: 17  PFLIKTYDMVDDSATDSIVSWTSSNKSFVVWNPPEFARLLLPTYFKHNNFSSFIRQLNTY 76
           PFL KTYDMVDD  T+ +VSW+S N SFVVW+ PEF+++LLP YFKHNNFSSF+RQLNTY
Sbjct: 27  PFLSKTYDMVDDPLTNEVVSWSSGNNSFVVWSAPEFSKVLLPKYFKHNNFSSFVRQLNTY 86

Query: 77  GFRKIDSERWEFANEDFIKDQKHLLKNIHRRKPIHSHSNPQ---------GSLVDPERAA 136
           GFRK+D +RWEFANE F++ +K LLK+I RRKP H   N Q         G+ V+  +  
Sbjct: 87  GFRKVDPDRWEFANEGFLRGRKQLLKSIVRRKPSHVQQNQQQTQVQSSSVGACVEVGKFG 146

Query: 137 FEDEIERLSREKATLEANISRFKQQKSTAKHQVRDLTVKVESMEKRQKSLLAFLEKAVQN 196
            E+E+ERL R+K  L   + R +QQ+   ++Q++++  KV+ ME+RQ+ +++FL KAVQ+
Sbjct: 147 IEEEVERLKRDKNVLMQELVRLRQQQQATENQLQNVGQKVQVMEQRQQQMMSFLAKAVQS 206

Query: 197 PSFVEHLARRVE---SMDFTAFNKKRRLP 214
           P F+  L ++     +      NKKRRLP
Sbjct: 207 PGFLNQLVQQNNNDGNRQIPGSNKKRRLP 235

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022146890.11.4e-25999.36heat stress transcription factor A-5 [Momordica charantia][more]
XP_022974752.16.0e-22385.84heat stress transcription factor A-5-like [Cucurbita maxima] >XP_022974753.1 hea... [more]
XP_022948442.11.0e-22285.65heat stress transcription factor A-5-like [Cucurbita moschata][more]
XP_038906704.11.9e-22186.29heat stress transcription factor A-5 [Benincasa hispida][more]
XP_023539978.13.7e-22084.81heat stress transcription factor A-5-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q94BZ55.8e-12052.98Heat stress transcription factor A-5 OS=Arabidopsis thaliana OX=3702 GN=HSFA5 PE... [more]
Q6K6S51.5e-9949.28Heat stress transcription factor A-5 OS=Oryza sativa subsp. japonica OX=39947 GN... [more]
O494038.6e-5544.57Heat stress transcription factor A-4a OS=Arabidopsis thaliana OX=3702 GN=HSFA4A ... [more]
Q84T614.3e-5450.22Heat stress transcription factor A-1 OS=Oryza sativa subsp. japonica OX=39947 GN... [more]
P411511.6e-5350.00Heat stress transcription factor A-1a OS=Arabidopsis thaliana OX=3702 GN=HSFA1A ... [more]
Match NameE-valueIdentityDescription
A0A6J1CYL36.7e-26099.36heat stress transcription factor A-5 OS=Momordica charantia OX=3673 GN=LOC111015... [more]
A0A6J1IB492.9e-22385.84heat stress transcription factor A-5-like OS=Cucurbita maxima OX=3661 GN=LOC1114... [more]
A0A6J1G9835.0e-22385.65heat stress transcription factor A-5-like OS=Cucurbita moschata OX=3662 GN=LOC11... [more]
A0A1S3AWR22.6e-21985.26heat stress transcription factor A-5 OS=Cucumis melo OX=3656 GN=LOC103483459 PE=... [more]
A0A0A0L9B56.3e-21884.62HSF_DOMAIN domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G129630 ... [more]
Match NameE-valueIdentityDescription
AT4G13980.14.1e-12152.98winged-helix DNA-binding transcription factor family protein [more]
AT4G18880.16.1e-5644.57heat shock transcription factor A4A [more]
AT4G17750.11.2e-5450.00heat shock factor 1 [more]
AT5G16820.15.7e-5450.24heat shock factor 3 [more]
AT5G16820.25.7e-5450.24heat shock factor 3 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 125..173
NoneNo IPR availableGENE3D6.10.280.220coord: 93..211
e-value: 1.4E-5
score: 27.0
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 312..343
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 224..239
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 312..340
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 219..239
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 442..470
NoneNo IPR availablePANTHERPTHR10015:SF377HEAT STRESS TRANSCRIPTION FACTOR A-5coord: 11..467
IPR000232Heat shock factor (HSF)-type, DNA-bindingPRINTSPR00056HSFDOMAINcoord: 56..68
score: 78.9
coord: 69..81
score: 81.76
coord: 18..41
score: 43.57
IPR000232Heat shock factor (HSF)-type, DNA-bindingSMARTSM00415hsfneu3coord: 14..107
e-value: 1.8E-61
score: 220.2
IPR000232Heat shock factor (HSF)-type, DNA-bindingPFAMPF00447HSF_DNA-bindcoord: 18..107
e-value: 7.5E-31
score: 106.6
IPR000232Heat shock factor (HSF)-type, DNA-bindingPROSITEPS00434HSF_DOMAINcoord: 57..81
IPR036388Winged helix-like DNA-binding domain superfamilyGENE3D1.10.10.10coord: 15..92
e-value: 3.0E-29
score: 102.9
IPR027725Heat shock transcription factor familyPANTHERPTHR10015HEAT SHOCK TRANSCRIPTION FACTORcoord: 11..467
IPR036390Winged helix DNA-binding domain superfamilySUPERFAMILY46785"Winged helix" DNA-binding domaincoord: 14..107

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS005643.1MS005643.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006355 regulation of transcription, DNA-templated
molecular_function GO:0003700 DNA-binding transcription factor activity
molecular_function GO:0043565 sequence-specific DNA binding