MS005447 (gene) Bitter gourd (TR) v1

BLAST of MS005447 vs. NCBI nr
Match: XP_022156962.1 (beta-amyrin 11-oxidase-like [Momordica charantia])

HSP 1 Score: 1000.3 bits (2585), Expect = 5.6e-288
Identity = 490/490 (100.00%), Postives = 490/490 (100.00%), Query Frame = 0

Query: 1   MEDLWVWLIGAVVGGYIFVFGILRNFNQWLFITRHRGRYHLPPGDMGWPIIGTLLPFLRA 60
           MEDLWVWLIGAVVGGYIFVFGILRNFNQWLFITRHRGRYHLPPGDMGWPIIGTLLPFLRA
Sbjct: 1   MEDLWVWLIGAVVGGYIFVFGILRNFNQWLFITRHRGRYHLPPGDMGWPIIGTLLPFLRA 60

Query: 61  FRSGRPDSFINHFVSKYGKIGMYKTHLFGSPSVIVCVPEVCRHVLMNDEQFVFGYSKATR 120
           FRSGRPDSFINHFVSKYGKIGMYKTHLFGSPSVIVCVPEVCRHVLMNDEQFVFGYSKATR
Sbjct: 61  FRSGRPDSFINHFVSKYGKIGMYKTHLFGSPSVIVCVPEVCRHVLMNDEQFVFGYSKATR 120

Query: 121 ILTGSKALNTVPKAEHRRLRRLIASLISGHDALSLYIGHVEGIVVTCLEEWGSMKKPVEF 180
           ILTGSKALNTVPKAEHRRLRRLIASLISGHDALSLYIGHVEGIVVTCLEEWGSMKKPVEF
Sbjct: 121 ILTGSKALNTVPKAEHRRLRRLIASLISGHDALSLYIGHVEGIVVTCLEEWGSMKKPVEF 180

Query: 181 LTEMKTVAFKVLLHIFIGSTSAAFIDKMEKLYTDFHLGFMASPIDLPGTTFSRALKARKE 240
           LTEMKTVAFKVLLHIFIGSTSAAFIDKMEKLYTDFHLGFMASPIDLPGTTFSRALKARKE
Sbjct: 181 LTEMKTVAFKVLLHIFIGSTSAAFIDKMEKLYTDFHLGFMASPIDLPGTTFSRALKARKE 240

Query: 241 LIKIFENVLKEKKANLGSKEVEKRKKDMTDLLLEVRDEDGQGLDDGCIIDLLIGFFFAGH 300
           LIKIFENVLKEKKANLGSKEVEKRKKDMTDLLLEVRDEDGQGLDDGCIIDLLIGFFFAGH
Sbjct: 241 LIKIFENVLKEKKANLGSKEVEKRKKDMTDLLLEVRDEDGQGLDDGCIIDLLIGFFFAGH 300

Query: 301 ETSAHSIMRAIMFLSENPETLLKAKAEQEKIVKARADAQQKGLTMKEIKQMDYLSKVIDE 360
           ETSAHSIMRAIMFLSENPETLLKAKAEQEKIVKARADAQQKGLTMKEIKQMDYLSKVIDE
Sbjct: 301 ETSAHSIMRAIMFLSENPETLLKAKAEQEKIVKARADAQQKGLTMKEIKQMDYLSKVIDE 360

Query: 361 TLRKTSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHMDPEIYENPQKFDPSRWDNP 420
           TLRKTSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHMDPEIYENPQKFDPSRWDNP
Sbjct: 361 TLRKTSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHMDPEIYENPQKFDPSRWDNP 420

Query: 421 KRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENHLPLPRPTD 480
           KRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENHLPLPRPTD
Sbjct: 421 KRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENHLPLPRPTD 480

Query: 481 NCLARVVRDS 491
           NCLARVVRDS
Sbjct: 481 NCLARVVRDS 490

BLAST of MS005447 vs. NCBI nr
Match: XP_038876914.1 (beta-amyrin 11-oxidase-like [Benincasa hispida])

HSP 1 Score: 907.9 bits (2345), Expect = 3.8e-260
Identity = 441/489 (90.18%), Postives = 467/489 (95.50%), Query Frame = 0

Query: 3   DLWVWLIGAVVGGYIFVFGILRNFNQWLFITRHRGRYHLPPGDMGWPIIGTLLPFLRAFR 62
           +LW+WLIGA+VG YIFVFGILRN NQW F+TRHR RY+LPPGDMGWP+IGTLLPFL+AFR
Sbjct: 17  ELWLWLIGALVGSYIFVFGILRNLNQWCFVTRHRRRYNLPPGDMGWPLIGTLLPFLQAFR 76

Query: 63  SGRPDSFINHFVSKYGKIGMYKTHLFGSPSVIVCVPEVCRHVLMNDEQFVFGYSKATRIL 122
           SGRPDSFI HF  KYGK+GMYKT+LFGSPSVIV VPEVCRHVLMNDEQ+ FGYSKATRIL
Sbjct: 77  SGRPDSFIQHFAYKYGKVGMYKTYLFGSPSVIVTVPEVCRHVLMNDEQYGFGYSKATRIL 136

Query: 123 TGSKALNTVPKAEHRRLRRLIASLISGHDALSLYIGHVEGIVVTCLEEWGSMKKPVEFLT 182
           TGSKALNTVPK EHRRLRRLIASLISG++ALSLYIGHVEGIV+ CLEEWG+MKKPVEFL+
Sbjct: 137 TGSKALNTVPKPEHRRLRRLIASLISGNEALSLYIGHVEGIVIKCLEEWGTMKKPVEFLS 196

Query: 183 EMKTVAFKVLLHIFIGSTSAAFIDKMEKLYTDFHLGFMASPIDLPGTTFSRALKARKELI 242
           EMKTVAFKVLLHIFIG+ +AAFID+MEKLYTDFHLGFM+SP+DLPGTTFSRALKAR ELI
Sbjct: 197 EMKTVAFKVLLHIFIGANTAAFIDRMEKLYTDFHLGFMSSPVDLPGTTFSRALKARNELI 256

Query: 243 KIFENVLKEKKANLGSKEVEKRKKDMTDLLLEVRDEDGQGLDDGCIIDLLIGFFFAGHET 302
           KIFENVLKEK+ANL +K  E RKKDMTDLLLEVRDEDGQGLDDGCIIDLLIGFFFAGHET
Sbjct: 257 KIFENVLKEKRANLKNKGEENRKKDMTDLLLEVRDEDGQGLDDGCIIDLLIGFFFAGHET 316

Query: 303 SAHSIMRAIMFLSENPETLLKAKAEQEKIVKAR-ADAQQKGLTMKEIKQMDYLSKVIDET 362
           SAHSIMRAIM+LSENPETL KAKAEQE+IVKAR ADAQ KGLTMKEIKQM+YL KVIDET
Sbjct: 317 SAHSIMRAIMYLSENPETLQKAKAEQEQIVKARPADAQHKGLTMKEIKQMEYLFKVIDET 376

Query: 363 LRKTSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHMDPEIYENPQKFDPSRWDNPK 422
           LRKTSLAFTLSRETKVDVNL GYTIPKGWKILVWTRAVHMDPEIYENPQKFDPSRWDNPK
Sbjct: 377 LRKTSLAFTLSRETKVDVNLKGYTIPKGWKILVWTRAVHMDPEIYENPQKFDPSRWDNPK 436

Query: 423 RRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENHLPLPRPTDN 482
           RRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPEN+LPLPRPTDN
Sbjct: 437 RRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENYLPLPRPTDN 496

Query: 483 CLARVVRDS 491
           CLARVVRDS
Sbjct: 497 CLARVVRDS 505

BLAST of MS005447 vs. NCBI nr
Match: XP_008465946.1 (PREDICTED: beta-amyrin 11-oxidase-like [Cucumis melo] >KAA0038547.1 beta-amyrin 11-oxidase-like [Cucumis melo var. makuwa])

HSP 1 Score: 894.4 bits (2310), Expect = 4.3e-256
Identity = 440/491 (89.61%), Postives = 461/491 (93.89%), Query Frame = 0

Query: 1   MEDLWVWLIGAVVGGYIFVFGILRNFNQWLFITRHRGRYHLPPGDMGWPIIGTLLPFLRA 60
           ME LW+ L+GA+V  YIFVF ILRN NQW F+TRH  RY+LPPGDMGWP+IGTLLPFL+A
Sbjct: 1   MEQLWLSLLGALVASYIFVFLILRNLNQWWFVTRHGRRYNLPPGDMGWPLIGTLLPFLQA 60

Query: 61  FRSGRPDSFINHFVSKYGKIGMYKTHLFGSPSVIVCVPEVCRHVLMNDEQFVFGYSKATR 120
           FRSGRPDSFI+ F  KYGKIGMYKT+LFGSPSVIV VPEVCRHVLMNDEQF FGYSKATR
Sbjct: 61  FRSGRPDSFIHLFAYKYGKIGMYKTYLFGSPSVIVTVPEVCRHVLMNDEQFGFGYSKATR 120

Query: 121 ILTGSKALNTVPKAEHRRLRRLIASLISGHDALSLYIGHVEGIVVTCLEEWGSMKKPVEF 180
           ILTGSKALNTVPK EHRRLRRLIASLISG++ALSLYIGHVEGIVVTCLEEW SMKKPVEF
Sbjct: 121 ILTGSKALNTVPKPEHRRLRRLIASLISGNEALSLYIGHVEGIVVTCLEEWASMKKPVEF 180

Query: 181 LTEMKTVAFKVLLHIFIGSTSAAFIDKMEKLYTDFHLGFMASPIDLPGTTFSRALKARKE 240
           L+EMKTVAFKVLLHIFIG+ +AAFID+MEKLY DFHLGFM+SP+DLPGTTFSRALKAR E
Sbjct: 181 LSEMKTVAFKVLLHIFIGANTAAFIDRMEKLYNDFHLGFMSSPVDLPGTTFSRALKARNE 240

Query: 241 LIKIFENVLKEKKANLGSKEVEKRKKDMTDLLLEVRDEDGQGLDDGCIIDLLIGFFFAGH 300
           LIKIFENVLKEK+ NL  K  E RKKDMTDLLLEVRDEDGQGLDDGCIIDLLIGFFFAGH
Sbjct: 241 LIKIFENVLKEKRENLKKKVAENRKKDMTDLLLEVRDEDGQGLDDGCIIDLLIGFFFAGH 300

Query: 301 ETSAHSIMRAIMFLSENPETLLKAKAEQEKIVKAR-ADAQQKGLTMKEIKQMDYLSKVID 360
           ETSAHSIMRAIMFLSENPETL KAKAEQE+IVKAR ADAQ KGLTMKEIKQM+YL KVID
Sbjct: 301 ETSAHSIMRAIMFLSENPETLQKAKAEQEQIVKARPADAQHKGLTMKEIKQMEYLFKVID 360

Query: 361 ETLRKTSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHMDPEIYENPQKFDPSRWDN 420
           ETLRKTSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHMDPEIYENPQKFDPSRW+N
Sbjct: 361 ETLRKTSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHMDPEIYENPQKFDPSRWEN 420

Query: 421 PKRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENHLPLPRPT 480
           PKRR GSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENHLPLPRPT
Sbjct: 421 PKRRGGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENHLPLPRPT 480

Query: 481 DNCLARVVRDS 491
           DNCLARVVRDS
Sbjct: 481 DNCLARVVRDS 491

BLAST of MS005447 vs. NCBI nr
Match: XP_022995340.1 (beta-amyrin 11-oxidase-like [Cucurbita maxima])

HSP 1 Score: 891.0 bits (2301), Expect = 4.8e-255
Identity = 433/489 (88.55%), Postives = 462/489 (94.48%), Query Frame = 0

Query: 3   DLWVWLIGAVVGGYIFVFGILRNFNQWLFITRHRGRYHLPPGDMGWPIIGTLLPFLRAFR 62
           D+W+ LIGA+ G Y+FVFGILRN NQW F+TRH  RY+LPPGDMGWP+IGTLLPFL+AFR
Sbjct: 4   DIWLCLIGALAGVYVFVFGILRNLNQWRFVTRHGRRYNLPPGDMGWPLIGTLLPFLKAFR 63

Query: 63  SGRPDSFINHFVSKYGKIGMYKTHLFGSPSVIVCVPEVCRHVLMNDEQFVFGYSKATRIL 122
           SGRPDSFI+HF SKYGK GMYKTHLFGSPSVIV VPEVCRHVLMNDEQFVFGYSKATRIL
Sbjct: 64  SGRPDSFIHHFTSKYGKTGMYKTHLFGSPSVIVTVPEVCRHVLMNDEQFVFGYSKATRIL 123

Query: 123 TGSKALNTVPKAEHRRLRRLIASLISGHDALSLYIGHVEGIVVTCLEEWGSMKKPVEFLT 182
           TGSKALN+VPK EHRRLRRLIASLISG++ALSLYIGHVEGIVVTCLEEWGSMK+PVEFL+
Sbjct: 124 TGSKALNSVPKPEHRRLRRLIASLISGNEALSLYIGHVEGIVVTCLEEWGSMKEPVEFLS 183

Query: 183 EMKTVAFKVLLHIFIGSTSAAFIDKMEKLYTDFHLGFMASPIDLPGTTFSRALKARKELI 242
           EMKTVAFKVLLHIFIG+ SAAFIDKMEKLY DFHLGFM++ +D+PGTTFSRALKAR ELI
Sbjct: 184 EMKTVAFKVLLHIFIGANSAAFIDKMEKLYNDFHLGFMSTAVDIPGTTFSRALKARNELI 243

Query: 243 KIFENVLKEKKANLGSKEVEKRKKDMTDLLLEVRDEDGQGLDDGCIIDLLIGFFFAGHET 302
           +IFE VL+EK+ NL +K  E RKKDM DLLLEVRDEDG+GLDDGCIIDLLIGFFFAGHET
Sbjct: 244 RIFEKVLEEKRENLKNKVGENRKKDMADLLLEVRDEDGEGLDDGCIIDLLIGFFFAGHET 303

Query: 303 SAHSIMRAIMFLSENPETLLKAKAEQEKIVKAR-ADAQQKGLTMKEIKQMDYLSKVIDET 362
           SAH+IMRAIMFLSENPETLLKAKAEQEKIV AR ADAQ KGLTMKEIKQM+YLSKV+DET
Sbjct: 304 SAHTIMRAIMFLSENPETLLKAKAEQEKIVNARPADAQHKGLTMKEIKQMEYLSKVMDET 363

Query: 363 LRKTSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHMDPEIYENPQKFDPSRWDNPK 422
           LRKTSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHM+PEIYENP +FDPSRWDNPK
Sbjct: 364 LRKTSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHMNPEIYENPHQFDPSRWDNPK 423

Query: 423 RRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENHLPLPRPTDN 482
           RRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENHLPLP PTDN
Sbjct: 424 RRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENHLPLPTPTDN 483

Query: 483 CLARVVRDS 491
           CLARVVRDS
Sbjct: 484 CLARVVRDS 492

BLAST of MS005447 vs. NCBI nr
Match: XP_022971975.1 (beta-amyrin 11-oxidase-like [Cucurbita maxima])

HSP 1 Score: 889.0 bits (2296), Expect = 1.8e-254
Identity = 423/489 (86.50%), Postives = 465/489 (95.09%), Query Frame = 0

Query: 3   DLWVWLIGAVVGGYIFVFGILRNFNQWLFITRHRGRYHLPPGDMGWPIIGTLLPFLRAFR 62
           ++WVWL+GA+VG Y+FVFGILRN NQW F+TRH GRY+LPPGDMGWP++GTLLPFL+AFR
Sbjct: 2   EVWVWLVGALVGAYVFVFGILRNLNQWRFVTRHGGRYNLPPGDMGWPLVGTLLPFLQAFR 61

Query: 63  SGRPDSFINHFVSKYGKIGMYKTHLFGSPSVIVCVPEVCRHVLMNDEQFVFGYSKATRIL 122
           SGRPDSFI+HF S+YGKIGMYKT+LFGSPSVIVCVPEVCRHVLMNDEQF FGYSKATRIL
Sbjct: 62  SGRPDSFIHHFASQYGKIGMYKTYLFGSPSVIVCVPEVCRHVLMNDEQFAFGYSKATRIL 121

Query: 123 TGSKALNTVPKAEHRRLRRLIASLISGHDALSLYIGHVEGIVVTCLEEWGSMKKPVEFLT 182
           TGSKALNTV + EHRR+RRLIASLISG+DALS+YIGHVEGIV+TCLEEW +MKKPVEFL+
Sbjct: 122 TGSKALNTVSRPEHRRIRRLIASLISGNDALSIYIGHVEGIVITCLEEWATMKKPVEFLS 181

Query: 183 EMKTVAFKVLLHIFIGSTSAAFIDKMEKLYTDFHLGFMASPIDLPGTTFSRALKARKELI 242
           EMKT+AFK+LLHIFIG+ +AAFIDKMEKLYTDFHLGFM+S +D+PGTTFSRALKAR ELI
Sbjct: 182 EMKTMAFKILLHIFIGANTAAFIDKMEKLYTDFHLGFMSSAVDIPGTTFSRALKARNELI 241

Query: 243 KIFENVLKEKKANLGSKEVEKRKKDMTDLLLEVRDEDGQGLDDGCIIDLLIGFFFAGHET 302
           +IFE+VLKEK+ NL +K  E RKKDMTDLLLEV+DE+G GLDDGCIIDLLIGFFFAGHET
Sbjct: 242 RIFESVLKEKRENLKNKVEENRKKDMTDLLLEVKDEEGHGLDDGCIIDLLIGFFFAGHET 301

Query: 303 SAHSIMRAIMFLSENPETLLKAKAEQEKIVKARADAQQ-KGLTMKEIKQMDYLSKVIDET 362
           SAHSIMRAIMFLSENPETL KAKAEQEKIV AR  +++ +GLTMKEIKQM+YLSKVIDET
Sbjct: 302 SAHSIMRAIMFLSENPETLCKAKAEQEKIVNARPTSERHRGLTMKEIKQMEYLSKVIDET 361

Query: 363 LRKTSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHMDPEIYENPQKFDPSRWDNPK 422
           LRKTSLAFTLSRET+ DVNLNGYTIPKGWKILVWTRAVHMDPEIYENP++FDPSRWDNPK
Sbjct: 362 LRKTSLAFTLSRETRTDVNLNGYTIPKGWKILVWTRAVHMDPEIYENPEEFDPSRWDNPK 421

Query: 423 RRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENHLPLPRPTDN 482
           RRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPEN+LPLPRPTDN
Sbjct: 422 RRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENYLPLPRPTDN 481

Query: 483 CLARVVRDS 491
           CLARVVRDS
Sbjct: 482 CLARVVRDS 490

BLAST of MS005447 vs. ExPASy Swiss-Prot
Match: B5BSX1 (Beta-amyrin 11-oxidase OS=Glycyrrhiza uralensis OX=74613 GN=CYP88D6 PE=1 SV=1)

HSP 1 Score: 518.8 bits (1335), Expect = 6.5e-146
Identity = 258/493 (52.33%), Postives = 353/493 (71.60%), Query Frame = 0

Query: 1   MEDLWVWLIGA-VVGGYIFVFGILRNFNQWLFITRHRGRYH-LPPGDMGWPIIGTLLPFL 60
           ME  WV +  A ++  YIF    +RN N W +  + R + H LPPGDMGWP+IG LL F+
Sbjct: 1   MEVHWVCMSAATLLVCYIFGSKFVRNLNGWYYDVKLRRKEHPLPPGDMGWPLIGDLLSFI 60

Query: 61  RAFRSGRPDSFINHFVSKYGKIGMYKTHLFGSPSVIVCVPEVCRHVLMNDEQFVFGYSKA 120
           + F SG PDSFIN+ V KYG+ G+YKTHLFG+PS+IVC P++CR VL +D  F  GY K+
Sbjct: 61  KDFSSGHPDSFINNLVLKYGRSGIYKTHLFGNPSIIVCEPQMCRRVLTDDVNFKLGYPKS 120

Query: 121 TRILTGSKALNTVPKAEHRRLRRLIASLISGHDALSLYIGHVEGIVVTCLEEWGSMKKPV 180
            + L   + +  V  AEHR  RRLI S I GH AL++Y+  +E IV+  LEE  SMK PV
Sbjct: 121 IKELARCRPMIDVSNAEHRLFRRLITSPIVGHKALAMYLERLEEIVINSLEELSSMKHPV 180

Query: 181 EFLTEMKTVAFKVLLHIFIGSTSAAFIDKMEKLYTDFHLGFMASPIDLPGTTFSRALKAR 240
           E L EMK V+FK ++H+F+GS++   I K+   +TD + G  + PI++PG TF +AL+AR
Sbjct: 181 ELLKEMKKVSFKAIVHVFMGSSNQDIIKKIGSSFTDLYNGMFSIPINVPGFTFHKALEAR 240

Query: 241 KELIKIFENVLKEKKANLGSKEVE-KRKKDMTDLLLEVRDEDGQGLDDGCIIDLLIGFFF 300
           K+L KI + V+ E++  + +   E  ++KD+ D+LLEV+DE+G+ L+D  I DLLIG  F
Sbjct: 241 KKLAKIVQPVVDERRLMIENGPQEGSQRKDLIDILLEVKDENGRKLEDEDISDLLIGLLF 300

Query: 301 AGHETSAHSIMRAIMFLSENPETLLKAKAEQEKIVKARADAQQKGLTMKEIKQMDYLSKV 360
           AGHE++A S+M +I +L+++P  L KAK EQE+I + R  + QK L++KEIKQM YLS+V
Sbjct: 301 AGHESTATSLMWSITYLTQHPHILKKAKEEQEEITRTRF-SSQKQLSLKEIKQMVYLSQV 360

Query: 361 IDETLRKTSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHMDPEIYENPQKFDPSRW 420
           IDETLR  ++AF   RE   DVN+NGY IPKGW++L+W RA+HMD E Y NP++F+PSRW
Sbjct: 361 IDETLRCANIAFATFREATADVNINGYIIPKGWRVLIWARAIHMDSEYYPNPEEFNPSRW 420

Query: 421 DNPKRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENHLPLPR 480
           D+   +AG+F+PFGAG RLCPG DL+KLEI+IFLHYF+   +RLER+NP C    LP+ +
Sbjct: 421 DDYNAKAGTFLPFGAGSRLCPGADLAKLEISIFLHYFLR-NYRLERINPECHVTSLPVSK 480

Query: 481 PTDNCLARVVRDS 491
           PTDNCLA+V++ S
Sbjct: 481 PTDNCLAKVIKVS 491

BLAST of MS005447 vs. ExPASy Swiss-Prot
Match: O23051 (Ent-kaurenoic acid oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=KAO1 PE=1 SV=1)

HSP 1 Score: 467.6 bits (1202), Expect = 1.7e-130
Identity = 228/469 (48.61%), Postives = 331/469 (70.58%), Query Frame = 0

Query: 22  ILRNFNQWLF-ITRHRGRYHLPPGDMGWPIIGTLLPFLRAFRSGRPDSFINHFVSKYGKI 81
           ++R  N WL+  +    R++LPPGD+GWP IG +L FLRAF++  PDSF    + +YG  
Sbjct: 25  LVRKVNVWLYESSLGENRHYLPPGDLGWPFIGNMLSFLRAFKTSDPDSFTRTLIKRYGPK 84

Query: 82  GMYKTHLFGSPSVIVCVPEVCRHVLMNDEQFVFGYSKATRILTGSKALNTVPKAEHRRLR 141
           G+YK H+FG+PS+IV   + CR VL +D+ F  G+  +T  L G K+   +   EH+RLR
Sbjct: 85  GIYKAHMFGNPSIIVTTSDTCRRVLTDDDAFKPGWPTSTMELIGRKSFVGISFEEHKRLR 144

Query: 142 RLIASLISGHDALSLYIGHVEGIVVTCLEEWGSMKKPVEFLTEMKTVAFKVLLHIFIGST 201
           RL A+ ++GH+ALS YI ++E  V+T L++W  M +  EFLT ++ + F+++++IF+ S 
Sbjct: 145 RLTAAPVNGHEALSTYIPYIEENVITVLDKWTKMGE-FEFLTHLRKLTFRIIMYIFLSSE 204

Query: 202 SAAFIDKMEKLYTDFHLGFMASPIDLPGTTFSRALKARKELIKIFENVLKEKKANLGSKE 261
           S   +D +E+ YT  + G  A  +++PG  + RALKARK L+  F++++ E++ N   + 
Sbjct: 205 SENVMDALEREYTALNYGVRAMAVNIPGFAYHRALKARKTLVAAFQSIVTERR-NQRKQN 264

Query: 262 VEKRKKDMTDLLLEVRDEDGQGLDDGCIIDLLIGFFFAGHETSAHSIMRAIMFLSENPET 321
           +   KKDM D LL V+DEDG+ LDD  IID+L+ +  AGHE+S H+IM A +FL E+PE 
Sbjct: 265 ILSNKKDMLDNLLNVKDEDGKTLDDEEIIDVLLMYLNAGHESSGHTIMWATVFLQEHPEV 324

Query: 322 LLKAKAEQEKIVKARADAQQKGLTMKEIKQMDYLSKVIDETLRKTSLAFTLSRETKVDVN 381
           L +AKAEQE I+K+R +  QKGL++KE ++M++LS+V+DETLR  + + T  RE K DV 
Sbjct: 325 LQRAKAEQEMILKSRPEG-QKGLSLKETRKMEFLSQVVDETLRVITFSLTAFREAKTDVE 384

Query: 382 LNGYTIPKGWKILVWTRAVHMDPEIYENPQKFDPSRWDN---PKRRAGSFIPFGAGMRLC 441
           +NGY IPKGWK+L W R VH+DPE++ +P+KFDP+RWDN   PK  AG+F+PFGAG  LC
Sbjct: 385 MNGYLIPKGWKVLTWFRDVHIDPEVFPDPRKFDPARWDNGFVPK--AGAFLPFGAGSHLC 444

Query: 442 PGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENHLPLPRPTDNCLARV 487
           PG DL+KLEI+IFLH+F+   ++++R NP CP  +LP  RPTDNCLAR+
Sbjct: 445 PGNDLAKLEISIFLHHFLL-KYQVKRSNPECPVMYLPHTRPTDNCLARI 487

BLAST of MS005447 vs. ExPASy Swiss-Prot
Match: Q9C5Y2 (Ent-kaurenoic acid oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=KAO2 PE=1 SV=2)

HSP 1 Score: 461.8 bits (1187), Expect = 9.4e-129
Identity = 226/484 (46.69%), Postives = 335/484 (69.21%), Query Frame = 0

Query: 6   VWLIGAVVGGYIFVFGILRNFNQWLFITR-HRGRYHLPPGDMGWPIIGTLLPFLRAFRSG 65
           +W    ++G ++  + +L+  N W+++++    +++LPPGD+GWP+IG +  FLRAF++ 
Sbjct: 9   MWFPLIILGLFVLKW-VLKRVNVWIYVSKLGEKKHYLPPGDLGWPVIGNMWSFLRAFKTS 68

Query: 66  RPDSFINHFVSKYGKIGMYKTHLFGSPSVIVCVPEVCRHVLMNDEQFVFGYSKATRILTG 125
            P+SFI  ++++YG+ G+YK H+FG P V+V  PE CR VL +D+ F  G+ K+T  L G
Sbjct: 69  DPESFIQSYITRYGRTGIYKAHMFGYPCVLVTTPETCRRVLTDDDAFHIGWPKSTMKLIG 128

Query: 126 SKALNTVPKAEHRRLRRLIASLISGHDALSLYIGHVEGIVVTCLEEWGSMKKPVEFLTEM 185
            K+   +   EH+RLRRL ++ ++G +ALS+YI  +E  V T LE+W  M + +EFL+ +
Sbjct: 129 RKSFVGISFEEHKRLRRLTSAPVNGPEALSVYIQFIEETVNTDLEKWSKMGE-IEFLSHL 188

Query: 186 KTVAFKVLLHIFIGSTSAAFIDKMEKLYTDFHLGFMASPIDLPGTTFSRALKARKELIKI 245
           + + FKV+++IF+ S S   +D +E+ YT+ + G  A  I+LPG  + RALKARK+L+  
Sbjct: 189 RKLTFKVIMYIFLSSESEHVMDSLEREYTNLNYGVRAMGINLPGFAYHRALKARKKLVAA 248

Query: 246 FENVLKEKKANLGSKEVEKRKKDMTDLLLEVRDEDGQGLDDGCIIDLLIGFFFAGHETSA 305
           F++++  ++ N   + +   +KDM D L++V+DE+G+ LDD  IIDLL+ +  AGHE+S 
Sbjct: 249 FQSIVTNRR-NQRKQNISSNRKDMLDNLIDVKDENGRVLDDEEIIDLLLMYLNAGHESSG 308

Query: 306 HSIMRAIMFLSENPETLLKAKAEQEKIVKARADAQQKGLTMKEIKQMDYLSKVIDETLRK 365
           H  M A + + E+P  L KAK EQE+IVK RA  Q+  LT+KE ++M YLS+VIDETLR 
Sbjct: 309 HLTMWATILMQEHPMILQKAKEEQERIVKKRAPGQK--LTLKETREMVYLSQVIDETLRV 368

Query: 366 TSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHMDPEIYENPQKFDPSRWDNPKRRA 425
            + + T  RE K DV ++GY IPKGWK+L W R VH+DPEIY +P+KFDPSRW+    +A
Sbjct: 369 ITFSLTAFREAKSDVQMDGYIIPKGWKVLTWFRNVHLDPEIYPDPKKFDPSRWEGYTPKA 428

Query: 426 GSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENHLPLPRPTDNCLA 485
           G+F+PFG G  LCPG DL+KLEI+IFLH+F+   +R+ER NP CP   LP  RP DNCLA
Sbjct: 429 GTFLPFGLGSHLCPGNDLAKLEISIFLHHFLL-KYRVERSNPGCPVMFLPHNRPKDNCLA 486

Query: 486 RVVR 489
           R+ R
Sbjct: 489 RITR 486

BLAST of MS005447 vs. ExPASy Swiss-Prot
Match: Q9AXH9 (Ent-kaurenoic acid oxidase 1 OS=Hordeum vulgare OX=4513 GN=KAO1 PE=1 SV=1)

HSP 1 Score: 455.3 bits (1170), Expect = 8.8e-127
Identity = 229/496 (46.17%), Postives = 324/496 (65.32%), Query Frame = 0

Query: 2   EDLWVWLIGAVVGGYIFVF--GILRNFNQWLFITR----HRGRYHLPPGDMGWPIIGTLL 61
           E  W W + AVV     V     +R  + W +        RGR  LPPGDMGWP++G + 
Sbjct: 3   EGAW-WAVAAVVAALAVVALDAAVRAAHAWYWTASLGAGRRGR--LPPGDMGWPLVGGMW 62

Query: 62  PFLRAFRSGRPDSFINHFVSKYGKIGMYKTHLFGSPSVIVCVPEVCRHVLMNDEQFVFGY 121
            FLRAF+SGRPDSFI+ F  ++G+ G+Y+  +F SP+++   PE C+ VLM+D+ FV G+
Sbjct: 63  AFLRAFKSGRPDSFIDSFARRFGRAGLYRAFMFSSPTIMATTPEACKQVLMDDDAFVTGW 122

Query: 122 SKATRILTGSKALNTVPKAEHRRLRRLIASLISGHDALSLYIGHVEGIVVTCLEEWGSM- 181
            KAT  L G K+   +   EHRRLR+L A+ I+G DAL+ Y+G ++  VVT L  W    
Sbjct: 123 PKATVALIGPKSFVNMGYDEHRRLRKLTAAPINGFDALTSYLGFIDDTVVTTLRGWSERG 182

Query: 182 -KKPVEFLTEMKTVAFKVLLHIFIGSTSAAFIDKMEKLYTDFHLGFMASPIDLPGTTFSR 241
                EFLTE++ + F++++ IF+G        ++E+ YT+ + G  A  IDLPG  + +
Sbjct: 183 GDGHFEFLTELRRMTFRIIVQIFMGGADERTAAELERTYTELNYGMRAMAIDLPGFAYHK 242

Query: 242 ALKARKELIKIFENVLKEKKANLGSKEV-EKRKKDMTDLLLEVRDEDGQGLDDGCIIDLL 301
           A++AR+ L+   + VL E++A  G          DM D L+ V DE G+ L D  IID+L
Sbjct: 243 AIRARRRLVAALQRVLDERRARGGKTAAGAAAPVDMMDRLIAVEDEGGRRLQDDEIIDVL 302

Query: 302 IGFFFAGHETSAHSIMRAIMFLSENPETLLKAKAEQEKIVKARADAQQKGLTMKEIKQMD 361
           + +  AGHE+S H  M A +FL ENPE L KAKAEQE I+++     QKGLT+++ ++M 
Sbjct: 303 VMYLNAGHESSGHITMWATVFLQENPEILAKAKAEQEAIMRS-IPPGQKGLTLRDFRKMA 362

Query: 362 YLSKVIDETLRKTSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHMDPEIYENPQKF 421
           YLS+V+DETLR  +++F   R+   DV +NGY IPKGWK+ +W R+VHMDP++Y +P+KF
Sbjct: 363 YLSQVVDETLRFVNISFVSFRQATRDVFVNGYLIPKGWKVQLWYRSVHMDPQVYPDPKKF 422

Query: 422 DPSRWDNPKRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENH 481
           DPSRW+ P  RAG+F+PFG G RLCPG DL+KLEI++FLH+F+  G++L R NPNC   +
Sbjct: 423 DPSRWEGPPPRAGTFLPFGLGTRLCPGNDLAKLEISVFLHHFLL-GYKLTRKNPNCRVRY 482

Query: 482 LPLPRPTDNCLARVVR 489
           LP PRP DNCLA++ R
Sbjct: 483 LPHPRPVDNCLAKITR 493

BLAST of MS005447 vs. ExPASy Swiss-Prot
Match: Q5VRM7 (Ent-kaurenoic acid oxidase OS=Oryza sativa subsp. japonica OX=39947 GN=KAO PE=2 SV=1)

HSP 1 Score: 455.3 bits (1170), Expect = 8.8e-127
Identity = 219/491 (44.60%), Postives = 328/491 (66.80%), Query Frame = 0

Query: 3   DLWVWLIGAVVGGYIFVFGILRNFNQWLFITR--HRGRYHLPPGDMGWPIIGTLLPFLRA 62
           DLWV L  AVV G + V  ++R  + W+ +       R  LPPG+MGWP++G++  FLRA
Sbjct: 16  DLWV-LAAAVVAGVVLVDAVVRRAHDWVRVAALGAERRSRLPPGEMGWPMVGSMWAFLRA 75

Query: 63  FRSGRPDSFINHFVSKYGKIGMYKTHLFGSPSVIVCVPEVCRHVLMNDEQFVFGYSKATR 122
           F+SG PD+FI  F+ ++G+ G+Y+T +F SP+++   PE C+ VLM+DE FV G+ KAT 
Sbjct: 76  FKSGNPDAFIASFIRRFGRTGVYRTFMFSSPTILAVTPEACKQVLMDDEGFVTGWPKATV 135

Query: 123 ILTGSKALNTVPKAEHRRLRRLIASLISGHDALSLYIGHVEGIVVTCLEEWGSMKK-PVE 182
            L G K+   +   +HRR+R+L A+ I+G DAL+ Y+  ++  VV  L  W S +   VE
Sbjct: 136 TLIGPKSFVNMSYDDHRRIRKLTAAPINGFDALTTYLSFIDQTVVASLRRWSSPESGQVE 195

Query: 183 FLTEMKTVAFKVLLHIFIGSTSAAFIDKMEKLYTDFHLGFMASPIDLPGTTFSRALKARK 242
           FLTE++ + FK+++ IF+     A ++ +E+ YTD + G  A  I+LPG  + RAL+AR+
Sbjct: 196 FLTELRRMTFKIIVQIFMSGADDATMEALERSYTDLNYGMRAMAINLPGFAYYRALRARR 255

Query: 243 ELIKIFENVLKEKKANLGSKEVEKRKKDMTDLLLEVRDEDGQGLDDGCIIDLLIGFFFAG 302
           +L+ + + VL  ++A            DM D L+E  DE G+ L D  I+D+LI +  AG
Sbjct: 256 KLVSVLQGVLDGRRAAAAKGFKRSGAMDMMDRLIEAEDERGRRLADDEIVDVLIMYLNAG 315

Query: 303 HETSAHSIMRAIMFLSENPETLLKAKAEQEKIVKARADAQQKGLTMKEIKQMDYLSKVID 362
           HE+S H  M A +FL ENP+   +AKAEQE+I+++   A Q GLT+++ K+M +LS+V+D
Sbjct: 316 HESSGHITMWATVFLQENPDIFARAKAEQEEIMRS-IPATQNGLTLRDFKKMHFLSQVVD 375

Query: 363 ETLRKTSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHMDPEIYENPQKFDPSRWDN 422
           ETLR  +++F   R+   D+ +NGY IPKGWK+ +W R+VHMD ++Y +P+ F+PSRW+ 
Sbjct: 376 ETLRCVNISFVSFRQATRDIFVNGYLIPKGWKVQLWYRSVHMDDQVYPDPKMFNPSRWEG 435

Query: 423 PKRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENHLPLPRPT 482
           P  +AG+F+PFG G RLCPG DL+KLEI++FLH+F+  G++L+R NP C   +LP PRP 
Sbjct: 436 PPPKAGTFLPFGLGARLCPGNDLAKLEISVFLHHFLL-GYKLKRANPKCRVRYLPHPRPV 495

Query: 483 DNCLARVVRDS 491
           DNCLA + + S
Sbjct: 496 DNCLATITKVS 503

BLAST of MS005447 vs. ExPASy TrEMBL
Match: A0A6J1DTB9 (beta-amyrin 11-oxidase-like OS=Momordica charantia OX=3673 GN=LOC111023787 PE=3 SV=1)

HSP 1 Score: 1000.3 bits (2585), Expect = 2.7e-288
Identity = 490/490 (100.00%), Postives = 490/490 (100.00%), Query Frame = 0

Query: 1   MEDLWVWLIGAVVGGYIFVFGILRNFNQWLFITRHRGRYHLPPGDMGWPIIGTLLPFLRA 60
           MEDLWVWLIGAVVGGYIFVFGILRNFNQWLFITRHRGRYHLPPGDMGWPIIGTLLPFLRA
Sbjct: 1   MEDLWVWLIGAVVGGYIFVFGILRNFNQWLFITRHRGRYHLPPGDMGWPIIGTLLPFLRA 60

Query: 61  FRSGRPDSFINHFVSKYGKIGMYKTHLFGSPSVIVCVPEVCRHVLMNDEQFVFGYSKATR 120
           FRSGRPDSFINHFVSKYGKIGMYKTHLFGSPSVIVCVPEVCRHVLMNDEQFVFGYSKATR
Sbjct: 61  FRSGRPDSFINHFVSKYGKIGMYKTHLFGSPSVIVCVPEVCRHVLMNDEQFVFGYSKATR 120

Query: 121 ILTGSKALNTVPKAEHRRLRRLIASLISGHDALSLYIGHVEGIVVTCLEEWGSMKKPVEF 180
           ILTGSKALNTVPKAEHRRLRRLIASLISGHDALSLYIGHVEGIVVTCLEEWGSMKKPVEF
Sbjct: 121 ILTGSKALNTVPKAEHRRLRRLIASLISGHDALSLYIGHVEGIVVTCLEEWGSMKKPVEF 180

Query: 181 LTEMKTVAFKVLLHIFIGSTSAAFIDKMEKLYTDFHLGFMASPIDLPGTTFSRALKARKE 240
           LTEMKTVAFKVLLHIFIGSTSAAFIDKMEKLYTDFHLGFMASPIDLPGTTFSRALKARKE
Sbjct: 181 LTEMKTVAFKVLLHIFIGSTSAAFIDKMEKLYTDFHLGFMASPIDLPGTTFSRALKARKE 240

Query: 241 LIKIFENVLKEKKANLGSKEVEKRKKDMTDLLLEVRDEDGQGLDDGCIIDLLIGFFFAGH 300
           LIKIFENVLKEKKANLGSKEVEKRKKDMTDLLLEVRDEDGQGLDDGCIIDLLIGFFFAGH
Sbjct: 241 LIKIFENVLKEKKANLGSKEVEKRKKDMTDLLLEVRDEDGQGLDDGCIIDLLIGFFFAGH 300

Query: 301 ETSAHSIMRAIMFLSENPETLLKAKAEQEKIVKARADAQQKGLTMKEIKQMDYLSKVIDE 360
           ETSAHSIMRAIMFLSENPETLLKAKAEQEKIVKARADAQQKGLTMKEIKQMDYLSKVIDE
Sbjct: 301 ETSAHSIMRAIMFLSENPETLLKAKAEQEKIVKARADAQQKGLTMKEIKQMDYLSKVIDE 360

Query: 361 TLRKTSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHMDPEIYENPQKFDPSRWDNP 420
           TLRKTSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHMDPEIYENPQKFDPSRWDNP
Sbjct: 361 TLRKTSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHMDPEIYENPQKFDPSRWDNP 420

Query: 421 KRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENHLPLPRPTD 480
           KRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENHLPLPRPTD
Sbjct: 421 KRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENHLPLPRPTD 480

Query: 481 NCLARVVRDS 491
           NCLARVVRDS
Sbjct: 481 NCLARVVRDS 490

BLAST of MS005447 vs. ExPASy TrEMBL
Match: A0A5A7TAG0 (Beta-amyrin 11-oxidase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold92G00740 PE=3 SV=1)

HSP 1 Score: 894.4 bits (2310), Expect = 2.1e-256
Identity = 440/491 (89.61%), Postives = 461/491 (93.89%), Query Frame = 0

Query: 1   MEDLWVWLIGAVVGGYIFVFGILRNFNQWLFITRHRGRYHLPPGDMGWPIIGTLLPFLRA 60
           ME LW+ L+GA+V  YIFVF ILRN NQW F+TRH  RY+LPPGDMGWP+IGTLLPFL+A
Sbjct: 1   MEQLWLSLLGALVASYIFVFLILRNLNQWWFVTRHGRRYNLPPGDMGWPLIGTLLPFLQA 60

Query: 61  FRSGRPDSFINHFVSKYGKIGMYKTHLFGSPSVIVCVPEVCRHVLMNDEQFVFGYSKATR 120
           FRSGRPDSFI+ F  KYGKIGMYKT+LFGSPSVIV VPEVCRHVLMNDEQF FGYSKATR
Sbjct: 61  FRSGRPDSFIHLFAYKYGKIGMYKTYLFGSPSVIVTVPEVCRHVLMNDEQFGFGYSKATR 120

Query: 121 ILTGSKALNTVPKAEHRRLRRLIASLISGHDALSLYIGHVEGIVVTCLEEWGSMKKPVEF 180
           ILTGSKALNTVPK EHRRLRRLIASLISG++ALSLYIGHVEGIVVTCLEEW SMKKPVEF
Sbjct: 121 ILTGSKALNTVPKPEHRRLRRLIASLISGNEALSLYIGHVEGIVVTCLEEWASMKKPVEF 180

Query: 181 LTEMKTVAFKVLLHIFIGSTSAAFIDKMEKLYTDFHLGFMASPIDLPGTTFSRALKARKE 240
           L+EMKTVAFKVLLHIFIG+ +AAFID+MEKLY DFHLGFM+SP+DLPGTTFSRALKAR E
Sbjct: 181 LSEMKTVAFKVLLHIFIGANTAAFIDRMEKLYNDFHLGFMSSPVDLPGTTFSRALKARNE 240

Query: 241 LIKIFENVLKEKKANLGSKEVEKRKKDMTDLLLEVRDEDGQGLDDGCIIDLLIGFFFAGH 300
           LIKIFENVLKEK+ NL  K  E RKKDMTDLLLEVRDEDGQGLDDGCIIDLLIGFFFAGH
Sbjct: 241 LIKIFENVLKEKRENLKKKVAENRKKDMTDLLLEVRDEDGQGLDDGCIIDLLIGFFFAGH 300

Query: 301 ETSAHSIMRAIMFLSENPETLLKAKAEQEKIVKAR-ADAQQKGLTMKEIKQMDYLSKVID 360
           ETSAHSIMRAIMFLSENPETL KAKAEQE+IVKAR ADAQ KGLTMKEIKQM+YL KVID
Sbjct: 301 ETSAHSIMRAIMFLSENPETLQKAKAEQEQIVKARPADAQHKGLTMKEIKQMEYLFKVID 360

Query: 361 ETLRKTSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHMDPEIYENPQKFDPSRWDN 420
           ETLRKTSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHMDPEIYENPQKFDPSRW+N
Sbjct: 361 ETLRKTSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHMDPEIYENPQKFDPSRWEN 420

Query: 421 PKRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENHLPLPRPT 480
           PKRR GSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENHLPLPRPT
Sbjct: 421 PKRRGGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENHLPLPRPT 480

Query: 481 DNCLARVVRDS 491
           DNCLARVVRDS
Sbjct: 481 DNCLARVVRDS 491

BLAST of MS005447 vs. ExPASy TrEMBL
Match: A0A1S3CQE4 (beta-amyrin 11-oxidase-like OS=Cucumis melo OX=3656 GN=LOC103503517 PE=3 SV=1)

HSP 1 Score: 894.4 bits (2310), Expect = 2.1e-256
Identity = 440/491 (89.61%), Postives = 461/491 (93.89%), Query Frame = 0

Query: 1   MEDLWVWLIGAVVGGYIFVFGILRNFNQWLFITRHRGRYHLPPGDMGWPIIGTLLPFLRA 60
           ME LW+ L+GA+V  YIFVF ILRN NQW F+TRH  RY+LPPGDMGWP+IGTLLPFL+A
Sbjct: 1   MEQLWLSLLGALVASYIFVFLILRNLNQWWFVTRHGRRYNLPPGDMGWPLIGTLLPFLQA 60

Query: 61  FRSGRPDSFINHFVSKYGKIGMYKTHLFGSPSVIVCVPEVCRHVLMNDEQFVFGYSKATR 120
           FRSGRPDSFI+ F  KYGKIGMYKT+LFGSPSVIV VPEVCRHVLMNDEQF FGYSKATR
Sbjct: 61  FRSGRPDSFIHLFAYKYGKIGMYKTYLFGSPSVIVTVPEVCRHVLMNDEQFGFGYSKATR 120

Query: 121 ILTGSKALNTVPKAEHRRLRRLIASLISGHDALSLYIGHVEGIVVTCLEEWGSMKKPVEF 180
           ILTGSKALNTVPK EHRRLRRLIASLISG++ALSLYIGHVEGIVVTCLEEW SMKKPVEF
Sbjct: 121 ILTGSKALNTVPKPEHRRLRRLIASLISGNEALSLYIGHVEGIVVTCLEEWASMKKPVEF 180

Query: 181 LTEMKTVAFKVLLHIFIGSTSAAFIDKMEKLYTDFHLGFMASPIDLPGTTFSRALKARKE 240
           L+EMKTVAFKVLLHIFIG+ +AAFID+MEKLY DFHLGFM+SP+DLPGTTFSRALKAR E
Sbjct: 181 LSEMKTVAFKVLLHIFIGANTAAFIDRMEKLYNDFHLGFMSSPVDLPGTTFSRALKARNE 240

Query: 241 LIKIFENVLKEKKANLGSKEVEKRKKDMTDLLLEVRDEDGQGLDDGCIIDLLIGFFFAGH 300
           LIKIFENVLKEK+ NL  K  E RKKDMTDLLLEVRDEDGQGLDDGCIIDLLIGFFFAGH
Sbjct: 241 LIKIFENVLKEKRENLKKKVAENRKKDMTDLLLEVRDEDGQGLDDGCIIDLLIGFFFAGH 300

Query: 301 ETSAHSIMRAIMFLSENPETLLKAKAEQEKIVKAR-ADAQQKGLTMKEIKQMDYLSKVID 360
           ETSAHSIMRAIMFLSENPETL KAKAEQE+IVKAR ADAQ KGLTMKEIKQM+YL KVID
Sbjct: 301 ETSAHSIMRAIMFLSENPETLQKAKAEQEQIVKARPADAQHKGLTMKEIKQMEYLFKVID 360

Query: 361 ETLRKTSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHMDPEIYENPQKFDPSRWDN 420
           ETLRKTSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHMDPEIYENPQKFDPSRW+N
Sbjct: 361 ETLRKTSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHMDPEIYENPQKFDPSRWEN 420

Query: 421 PKRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENHLPLPRPT 480
           PKRR GSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENHLPLPRPT
Sbjct: 421 PKRRGGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENHLPLPRPT 480

Query: 481 DNCLARVVRDS 491
           DNCLARVVRDS
Sbjct: 481 DNCLARVVRDS 491

BLAST of MS005447 vs. ExPASy TrEMBL
Match: A0A6J1K3T5 (beta-amyrin 11-oxidase-like OS=Cucurbita maxima OX=3661 GN=LOC111490917 PE=3 SV=1)

HSP 1 Score: 891.0 bits (2301), Expect = 2.3e-255
Identity = 433/489 (88.55%), Postives = 462/489 (94.48%), Query Frame = 0

Query: 3   DLWVWLIGAVVGGYIFVFGILRNFNQWLFITRHRGRYHLPPGDMGWPIIGTLLPFLRAFR 62
           D+W+ LIGA+ G Y+FVFGILRN NQW F+TRH  RY+LPPGDMGWP+IGTLLPFL+AFR
Sbjct: 4   DIWLCLIGALAGVYVFVFGILRNLNQWRFVTRHGRRYNLPPGDMGWPLIGTLLPFLKAFR 63

Query: 63  SGRPDSFINHFVSKYGKIGMYKTHLFGSPSVIVCVPEVCRHVLMNDEQFVFGYSKATRIL 122
           SGRPDSFI+HF SKYGK GMYKTHLFGSPSVIV VPEVCRHVLMNDEQFVFGYSKATRIL
Sbjct: 64  SGRPDSFIHHFTSKYGKTGMYKTHLFGSPSVIVTVPEVCRHVLMNDEQFVFGYSKATRIL 123

Query: 123 TGSKALNTVPKAEHRRLRRLIASLISGHDALSLYIGHVEGIVVTCLEEWGSMKKPVEFLT 182
           TGSKALN+VPK EHRRLRRLIASLISG++ALSLYIGHVEGIVVTCLEEWGSMK+PVEFL+
Sbjct: 124 TGSKALNSVPKPEHRRLRRLIASLISGNEALSLYIGHVEGIVVTCLEEWGSMKEPVEFLS 183

Query: 183 EMKTVAFKVLLHIFIGSTSAAFIDKMEKLYTDFHLGFMASPIDLPGTTFSRALKARKELI 242
           EMKTVAFKVLLHIFIG+ SAAFIDKMEKLY DFHLGFM++ +D+PGTTFSRALKAR ELI
Sbjct: 184 EMKTVAFKVLLHIFIGANSAAFIDKMEKLYNDFHLGFMSTAVDIPGTTFSRALKARNELI 243

Query: 243 KIFENVLKEKKANLGSKEVEKRKKDMTDLLLEVRDEDGQGLDDGCIIDLLIGFFFAGHET 302
           +IFE VL+EK+ NL +K  E RKKDM DLLLEVRDEDG+GLDDGCIIDLLIGFFFAGHET
Sbjct: 244 RIFEKVLEEKRENLKNKVGENRKKDMADLLLEVRDEDGEGLDDGCIIDLLIGFFFAGHET 303

Query: 303 SAHSIMRAIMFLSENPETLLKAKAEQEKIVKAR-ADAQQKGLTMKEIKQMDYLSKVIDET 362
           SAH+IMRAIMFLSENPETLLKAKAEQEKIV AR ADAQ KGLTMKEIKQM+YLSKV+DET
Sbjct: 304 SAHTIMRAIMFLSENPETLLKAKAEQEKIVNARPADAQHKGLTMKEIKQMEYLSKVMDET 363

Query: 363 LRKTSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHMDPEIYENPQKFDPSRWDNPK 422
           LRKTSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHM+PEIYENP +FDPSRWDNPK
Sbjct: 364 LRKTSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHMNPEIYENPHQFDPSRWDNPK 423

Query: 423 RRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENHLPLPRPTDN 482
           RRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENHLPLP PTDN
Sbjct: 424 RRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENHLPLPTPTDN 483

Query: 483 CLARVVRDS 491
           CLARVVRDS
Sbjct: 484 CLARVVRDS 492

BLAST of MS005447 vs. ExPASy TrEMBL
Match: A0A6J1I785 (beta-amyrin 11-oxidase-like OS=Cucurbita maxima OX=3661 GN=LOC111470633 PE=3 SV=1)

HSP 1 Score: 889.0 bits (2296), Expect = 8.8e-255
Identity = 423/489 (86.50%), Postives = 465/489 (95.09%), Query Frame = 0

Query: 3   DLWVWLIGAVVGGYIFVFGILRNFNQWLFITRHRGRYHLPPGDMGWPIIGTLLPFLRAFR 62
           ++WVWL+GA+VG Y+FVFGILRN NQW F+TRH GRY+LPPGDMGWP++GTLLPFL+AFR
Sbjct: 2   EVWVWLVGALVGAYVFVFGILRNLNQWRFVTRHGGRYNLPPGDMGWPLVGTLLPFLQAFR 61

Query: 63  SGRPDSFINHFVSKYGKIGMYKTHLFGSPSVIVCVPEVCRHVLMNDEQFVFGYSKATRIL 122
           SGRPDSFI+HF S+YGKIGMYKT+LFGSPSVIVCVPEVCRHVLMNDEQF FGYSKATRIL
Sbjct: 62  SGRPDSFIHHFASQYGKIGMYKTYLFGSPSVIVCVPEVCRHVLMNDEQFAFGYSKATRIL 121

Query: 123 TGSKALNTVPKAEHRRLRRLIASLISGHDALSLYIGHVEGIVVTCLEEWGSMKKPVEFLT 182
           TGSKALNTV + EHRR+RRLIASLISG+DALS+YIGHVEGIV+TCLEEW +MKKPVEFL+
Sbjct: 122 TGSKALNTVSRPEHRRIRRLIASLISGNDALSIYIGHVEGIVITCLEEWATMKKPVEFLS 181

Query: 183 EMKTVAFKVLLHIFIGSTSAAFIDKMEKLYTDFHLGFMASPIDLPGTTFSRALKARKELI 242
           EMKT+AFK+LLHIFIG+ +AAFIDKMEKLYTDFHLGFM+S +D+PGTTFSRALKAR ELI
Sbjct: 182 EMKTMAFKILLHIFIGANTAAFIDKMEKLYTDFHLGFMSSAVDIPGTTFSRALKARNELI 241

Query: 243 KIFENVLKEKKANLGSKEVEKRKKDMTDLLLEVRDEDGQGLDDGCIIDLLIGFFFAGHET 302
           +IFE+VLKEK+ NL +K  E RKKDMTDLLLEV+DE+G GLDDGCIIDLLIGFFFAGHET
Sbjct: 242 RIFESVLKEKRENLKNKVEENRKKDMTDLLLEVKDEEGHGLDDGCIIDLLIGFFFAGHET 301

Query: 303 SAHSIMRAIMFLSENPETLLKAKAEQEKIVKARADAQQ-KGLTMKEIKQMDYLSKVIDET 362
           SAHSIMRAIMFLSENPETL KAKAEQEKIV AR  +++ +GLTMKEIKQM+YLSKVIDET
Sbjct: 302 SAHSIMRAIMFLSENPETLCKAKAEQEKIVNARPTSERHRGLTMKEIKQMEYLSKVIDET 361

Query: 363 LRKTSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHMDPEIYENPQKFDPSRWDNPK 422
           LRKTSLAFTLSRET+ DVNLNGYTIPKGWKILVWTRAVHMDPEIYENP++FDPSRWDNPK
Sbjct: 362 LRKTSLAFTLSRETRTDVNLNGYTIPKGWKILVWTRAVHMDPEIYENPEEFDPSRWDNPK 421

Query: 423 RRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENHLPLPRPTDN 482
           RRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPEN+LPLPRPTDN
Sbjct: 422 RRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENYLPLPRPTDN 481

Query: 483 CLARVVRDS 491
           CLARVVRDS
Sbjct: 482 CLARVVRDS 490

BLAST of MS005447 vs. TAIR 10
Match: AT1G05160.1 (cytochrome P450, family 88, subfamily A, polypeptide 3 )

HSP 1 Score: 467.6 bits (1202), Expect = 1.2e-131
Identity = 228/469 (48.61%), Postives = 331/469 (70.58%), Query Frame = 0

Query: 22  ILRNFNQWLF-ITRHRGRYHLPPGDMGWPIIGTLLPFLRAFRSGRPDSFINHFVSKYGKI 81
           ++R  N WL+  +    R++LPPGD+GWP IG +L FLRAF++  PDSF    + +YG  
Sbjct: 25  LVRKVNVWLYESSLGENRHYLPPGDLGWPFIGNMLSFLRAFKTSDPDSFTRTLIKRYGPK 84

Query: 82  GMYKTHLFGSPSVIVCVPEVCRHVLMNDEQFVFGYSKATRILTGSKALNTVPKAEHRRLR 141
           G+YK H+FG+PS+IV   + CR VL +D+ F  G+  +T  L G K+   +   EH+RLR
Sbjct: 85  GIYKAHMFGNPSIIVTTSDTCRRVLTDDDAFKPGWPTSTMELIGRKSFVGISFEEHKRLR 144

Query: 142 RLIASLISGHDALSLYIGHVEGIVVTCLEEWGSMKKPVEFLTEMKTVAFKVLLHIFIGST 201
           RL A+ ++GH+ALS YI ++E  V+T L++W  M +  EFLT ++ + F+++++IF+ S 
Sbjct: 145 RLTAAPVNGHEALSTYIPYIEENVITVLDKWTKMGE-FEFLTHLRKLTFRIIMYIFLSSE 204

Query: 202 SAAFIDKMEKLYTDFHLGFMASPIDLPGTTFSRALKARKELIKIFENVLKEKKANLGSKE 261
           S   +D +E+ YT  + G  A  +++PG  + RALKARK L+  F++++ E++ N   + 
Sbjct: 205 SENVMDALEREYTALNYGVRAMAVNIPGFAYHRALKARKTLVAAFQSIVTERR-NQRKQN 264

Query: 262 VEKRKKDMTDLLLEVRDEDGQGLDDGCIIDLLIGFFFAGHETSAHSIMRAIMFLSENPET 321
           +   KKDM D LL V+DEDG+ LDD  IID+L+ +  AGHE+S H+IM A +FL E+PE 
Sbjct: 265 ILSNKKDMLDNLLNVKDEDGKTLDDEEIIDVLLMYLNAGHESSGHTIMWATVFLQEHPEV 324

Query: 322 LLKAKAEQEKIVKARADAQQKGLTMKEIKQMDYLSKVIDETLRKTSLAFTLSRETKVDVN 381
           L +AKAEQE I+K+R +  QKGL++KE ++M++LS+V+DETLR  + + T  RE K DV 
Sbjct: 325 LQRAKAEQEMILKSRPEG-QKGLSLKETRKMEFLSQVVDETLRVITFSLTAFREAKTDVE 384

Query: 382 LNGYTIPKGWKILVWTRAVHMDPEIYENPQKFDPSRWDN---PKRRAGSFIPFGAGMRLC 441
           +NGY IPKGWK+L W R VH+DPE++ +P+KFDP+RWDN   PK  AG+F+PFGAG  LC
Sbjct: 385 MNGYLIPKGWKVLTWFRDVHIDPEVFPDPRKFDPARWDNGFVPK--AGAFLPFGAGSHLC 444

Query: 442 PGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENHLPLPRPTDNCLARV 487
           PG DL+KLEI+IFLH+F+   ++++R NP CP  +LP  RPTDNCLAR+
Sbjct: 445 PGNDLAKLEISIFLHHFLL-KYQVKRSNPECPVMYLPHTRPTDNCLARI 487

BLAST of MS005447 vs. TAIR 10
Match: AT2G32440.1 (ent-kaurenoic acid hydroxylase 2 )

HSP 1 Score: 461.8 bits (1187), Expect = 6.7e-130
Identity = 226/484 (46.69%), Postives = 335/484 (69.21%), Query Frame = 0

Query: 6   VWLIGAVVGGYIFVFGILRNFNQWLFITR-HRGRYHLPPGDMGWPIIGTLLPFLRAFRSG 65
           +W    ++G ++  + +L+  N W+++++    +++LPPGD+GWP+IG +  FLRAF++ 
Sbjct: 9   MWFPLIILGLFVLKW-VLKRVNVWIYVSKLGEKKHYLPPGDLGWPVIGNMWSFLRAFKTS 68

Query: 66  RPDSFINHFVSKYGKIGMYKTHLFGSPSVIVCVPEVCRHVLMNDEQFVFGYSKATRILTG 125
            P+SFI  ++++YG+ G+YK H+FG P V+V  PE CR VL +D+ F  G+ K+T  L G
Sbjct: 69  DPESFIQSYITRYGRTGIYKAHMFGYPCVLVTTPETCRRVLTDDDAFHIGWPKSTMKLIG 128

Query: 126 SKALNTVPKAEHRRLRRLIASLISGHDALSLYIGHVEGIVVTCLEEWGSMKKPVEFLTEM 185
            K+   +   EH+RLRRL ++ ++G +ALS+YI  +E  V T LE+W  M + +EFL+ +
Sbjct: 129 RKSFVGISFEEHKRLRRLTSAPVNGPEALSVYIQFIEETVNTDLEKWSKMGE-IEFLSHL 188

Query: 186 KTVAFKVLLHIFIGSTSAAFIDKMEKLYTDFHLGFMASPIDLPGTTFSRALKARKELIKI 245
           + + FKV+++IF+ S S   +D +E+ YT+ + G  A  I+LPG  + RALKARK+L+  
Sbjct: 189 RKLTFKVIMYIFLSSESEHVMDSLEREYTNLNYGVRAMGINLPGFAYHRALKARKKLVAA 248

Query: 246 FENVLKEKKANLGSKEVEKRKKDMTDLLLEVRDEDGQGLDDGCIIDLLIGFFFAGHETSA 305
           F++++  ++ N   + +   +KDM D L++V+DE+G+ LDD  IIDLL+ +  AGHE+S 
Sbjct: 249 FQSIVTNRR-NQRKQNISSNRKDMLDNLIDVKDENGRVLDDEEIIDLLLMYLNAGHESSG 308

Query: 306 HSIMRAIMFLSENPETLLKAKAEQEKIVKARADAQQKGLTMKEIKQMDYLSKVIDETLRK 365
           H  M A + + E+P  L KAK EQE+IVK RA  Q+  LT+KE ++M YLS+VIDETLR 
Sbjct: 309 HLTMWATILMQEHPMILQKAKEEQERIVKKRAPGQK--LTLKETREMVYLSQVIDETLRV 368

Query: 366 TSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHMDPEIYENPQKFDPSRWDNPKRRA 425
            + + T  RE K DV ++GY IPKGWK+L W R VH+DPEIY +P+KFDPSRW+    +A
Sbjct: 369 ITFSLTAFREAKSDVQMDGYIIPKGWKVLTWFRNVHLDPEIYPDPKKFDPSRWEGYTPKA 428

Query: 426 GSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENHLPLPRPTDNCLA 485
           G+F+PFG G  LCPG DL+KLEI+IFLH+F+   +R+ER NP CP   LP  RP DNCLA
Sbjct: 429 GTFLPFGLGSHLCPGNDLAKLEISIFLHHFLL-KYRVERSNPGCPVMFLPHNRPKDNCLA 486

Query: 486 RVVR 489
           R+ R
Sbjct: 489 RITR 486

BLAST of MS005447 vs. TAIR 10
Match: AT2G32440.2 (ent-kaurenoic acid hydroxylase 2 )

HSP 1 Score: 461.8 bits (1187), Expect = 6.7e-130
Identity = 226/484 (46.69%), Postives = 335/484 (69.21%), Query Frame = 0

Query: 6   VWLIGAVVGGYIFVFGILRNFNQWLFITR-HRGRYHLPPGDMGWPIIGTLLPFLRAFRSG 65
           +W    ++G ++  + +L+  N W+++++    +++LPPGD+GWP+IG +  FLRAF++ 
Sbjct: 9   MWFPLIILGLFVLKW-VLKRVNVWIYVSKLGEKKHYLPPGDLGWPVIGNMWSFLRAFKTS 68

Query: 66  RPDSFINHFVSKYGKIGMYKTHLFGSPSVIVCVPEVCRHVLMNDEQFVFGYSKATRILTG 125
            P+SFI  ++++YG+ G+YK H+FG P V+V  PE CR VL +D+ F  G+ K+T  L G
Sbjct: 69  DPESFIQSYITRYGRTGIYKAHMFGYPCVLVTTPETCRRVLTDDDAFHIGWPKSTMKLIG 128

Query: 126 SKALNTVPKAEHRRLRRLIASLISGHDALSLYIGHVEGIVVTCLEEWGSMKKPVEFLTEM 185
            K+   +   EH+RLRRL ++ ++G +ALS+YI  +E  V T LE+W  M + +EFL+ +
Sbjct: 129 RKSFVGISFEEHKRLRRLTSAPVNGPEALSVYIQFIEETVNTDLEKWSKMGE-IEFLSHL 188

Query: 186 KTVAFKVLLHIFIGSTSAAFIDKMEKLYTDFHLGFMASPIDLPGTTFSRALKARKELIKI 245
           + + FKV+++IF+ S S   +D +E+ YT+ + G  A  I+LPG  + RALKARK+L+  
Sbjct: 189 RKLTFKVIMYIFLSSESEHVMDSLEREYTNLNYGVRAMGINLPGFAYHRALKARKKLVAA 248

Query: 246 FENVLKEKKANLGSKEVEKRKKDMTDLLLEVRDEDGQGLDDGCIIDLLIGFFFAGHETSA 305
           F++++  ++ N   + +   +KDM D L++V+DE+G+ LDD  IIDLL+ +  AGHE+S 
Sbjct: 249 FQSIVTNRR-NQRKQNISSNRKDMLDNLIDVKDENGRVLDDEEIIDLLLMYLNAGHESSG 308

Query: 306 HSIMRAIMFLSENPETLLKAKAEQEKIVKARADAQQKGLTMKEIKQMDYLSKVIDETLRK 365
           H  M A + + E+P  L KAK EQE+IVK RA  Q+  LT+KE ++M YLS+VIDETLR 
Sbjct: 309 HLTMWATILMQEHPMILQKAKEEQERIVKKRAPGQK--LTLKETREMVYLSQVIDETLRV 368

Query: 366 TSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHMDPEIYENPQKFDPSRWDNPKRRA 425
            + + T  RE K DV ++GY IPKGWK+L W R VH+DPEIY +P+KFDPSRW+    +A
Sbjct: 369 ITFSLTAFREAKSDVQMDGYIIPKGWKVLTWFRNVHLDPEIYPDPKKFDPSRWEGYTPKA 428

Query: 426 GSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENHLPLPRPTDNCLA 485
           G+F+PFG G  LCPG DL+KLEI+IFLH+F+   +R+ER NP CP   LP  RP DNCLA
Sbjct: 429 GTFLPFGLGSHLCPGNDLAKLEISIFLHHFLL-KYRVERSNPGCPVMFLPHNRPKDNCLA 486

Query: 486 RVVR 489
           R+ R
Sbjct: 489 RITR 486

BLAST of MS005447 vs. TAIR 10
Match: AT3G13730.1 (cytochrome P450, family 90, subfamily D, polypeptide 1 )

HSP 1 Score: 234.2 bits (596), Expect = 2.3e-61
Identity = 136/416 (32.69%), Postives = 223/416 (53.61%), Query Frame = 0

Query: 42  PPGDMGWPIIGTLLPFLRAFRSGRPDSFINHFVSKYGKIGMYKTHLFGSPSVIVCVPEVC 101
           P G +GWP+IG  + F+ +  S RP+SF++     YG++  +K+H+FG+ +++    EV 
Sbjct: 52  PHGSLGWPVIGETIEFVSSAYSDRPESFMDKRRLMYGRV--FKSHIFGTATIVSTDAEVN 111

Query: 102 RHVLMNDE-QFVFGYSKATRILTGSKALNTVPKAEHRRLRRLIASLISGHDALSLYIGHV 161
           R VL +D   FV  Y K  R L G  ++  +  + HRR   L+ S +      +  +  +
Sbjct: 112 RAVLQSDSTAFVPFYPKTVRELMGKSSILLINGSLHRRFHGLVGSFLKSPLLKAQIVRDM 171

Query: 162 EGIVVTCLEEWGSMKKPVEFLTEMKTVAFKVLLHIFIGSTSAAFIDKMEKLYTDFHLGFM 221
              +   ++ W S  +PV      KTVAFKVL    I       ++++++ + +F  G M
Sbjct: 172 HKFLSESMDLW-SEDQPVLLQDVSKTVAFKVLAKALISVEKGEDLEELKREFENFISGLM 231

Query: 222 ASPIDLPGTTFSRALKARKELIKIFENVLKEKKANLGSKEVEK-RKKDMTDLLLEVRDED 281
           + PI+ PGT   R+L+A+K ++K  E +++ K     +KE +    KD+ D+LL+   + 
Sbjct: 232 SLPINFPGTQLHRSLQAKKNMVKQVERIIEGKIRKTKNKEEDDVIAKDVVDVLLK---DS 291

Query: 282 GQGLDDGCIIDLLIGFFFAGHETSAHSIMRAIMFLSENPETLLKAKAEQEKIVKARADAQ 341
            + L    I + +I     GH++    I  A+ FLS++P  L     E  K+ K+  +  
Sbjct: 292 SEHLTHNLIANNMIDMMIPGHDSVPVLITLAVKFLSDSPAALNLLTEENMKL-KSLKELT 351

Query: 342 QKGLTMKEIKQMDYLSKVIDETLRKTSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAV 401
            + L   +   + +  KVI ETLR  ++   + R+   DV + GY IPKGW  L + R+V
Sbjct: 352 GEPLYWNDYLSLPFTQKVITETLRMGNVIIGVMRKAMKDVEIKGYVIPKGWCFLAYLRSV 411

Query: 402 HMDPEIYENPQKFDPSRWDNPKRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFI 456
           H+D   YE+P KF+P RW        SF PFG G RLCPG+DL++LE ++FLH+ +
Sbjct: 412 HLDKLYYESPYKFNPWRWQERDMNTSSFSPFGGGQRLCPGLDLARLETSVFLHHLV 460

BLAST of MS005447 vs. TAIR 10
Match: AT3G19270.1 (cytochrome P450, family 707, subfamily A, polypeptide 4 )

HSP 1 Score: 225.7 bits (574), Expect = 8.0e-59
Identity = 150/459 (32.68%), Postives = 234/459 (50.98%), Query Frame = 0

Query: 34  RHRGRYHLPPGDMGWPIIGTLLPFLRAFRSGRPDSFINHFVSKYGKIGMYKTHLFGSPSV 93
           +   R  LPPG MGWP +G  L       S  P+ F      +YG+I  +KT + G P V
Sbjct: 27  KKNSRGKLPPGSMGWPYLGETLQLY----SQNPNVFFTSKQKRYGEI--FKTRILGYPCV 86

Query: 94  IVCVPEVCRHVLM-NDEQFVFGYSKATRILTGSKALNTVPKAEHRRLRRLIASLISGHDA 153
           ++  PE  R VL+ +   F   Y ++   L G  AL       H  +R+L+ S     + 
Sbjct: 87  MLASPEAARFVLVTHAHMFKPTYPRSKEKLIGPSALFFHQGDYHSHIRKLVQSSFY-PET 146

Query: 154 LSLYIGHVEGIVVTCLEEWGSMKKPVEFLTEMKTVAFKVLLHIFIGSTSAAFIDKMEKLY 213
           +   I  +E I ++ L+ W +M   V    EMK  AF V +    G   +++ + ++  Y
Sbjct: 147 IRKLIPDIEHIALSSLQSWANM-PIVSTYQEMKKFAFDVGILAIFGHLESSYKEILKHNY 206

Query: 214 TDFHLGFMASPIDLPGTTFSRALKARKELIKIFENVLKEKKANLGSKEVEKRKKDMTDLL 273
                G+ + P+ LPGT++ +AL ARK+L  I   ++ E+      +E    + D    L
Sbjct: 207 NIVDKGYNSFPMSLPGTSYHKALMARKQLKTIVSEIICER------REKRALQTDFLGHL 266

Query: 274 LEVRDEDGQGLDDGCIIDLLIGFFFAGHETSAHSIMRAIMFLSENPETLLKAKAEQEKIV 333
           L  ++E G+ L    I D +IG  FA  +T+A  +   + +L ++ + L   KAEQ+ I 
Sbjct: 267 LNFKNEKGRVLTQEQIADNIIGVLFAAQDTTASCLTWILKYLHDDQKLLEAVKAEQKAIY 326

Query: 334 KARADAQQKGLTMKEIKQMDYLSKVIDETLRKTSLAFTLSRETKVDVNLNGYTIPKGWKI 393
           +  +  ++K LT ++ + M    KVI E+LR  S+     RE  VDV   GY IPKGWK+
Sbjct: 327 EENS-REKKPLTWRQTRNMPLTHKVIVESLRMASIISFTFREAVVDVEYKGYLIPKGWKV 386

Query: 394 LVWTRAVHMDPEIYENPQKFDPSRWD-NPKRRAGSFIPFGAGMRLCPGIDLSKLEIAIFL 453
           +   R +H +P+ + NP+ FDPSR++ NPK    +F+PFG+G+  CPG +L+KL+I IFL
Sbjct: 387 MPLFRNIHHNPKYFSNPEVFDPSRFEVNPK--PNTFMPFGSGVHACPGNELAKLQILIFL 446

Query: 454 HYFIHGGFRLERVNPNCPENHLPLPRPTDNCLARVVRDS 491
           H+ +   FR E         + P P P +   A   R S
Sbjct: 447 HHLV-SNFRWEVKGGEKGIQYSPFPIPQNGLPATFRRHS 467