MS005445 (gene) Bitter gourd (TR) v1

BLAST of MS005445 vs. NCBI nr
Match: XP_022157134.1 (beta-amyrin 11-oxidase-like isoform X1 [Momordica charantia])

HSP 1 Score: 944.5 bits (2440), Expect = 3.4e-271
Identity = 462/462 (100.00%), Postives = 462/462 (100.00%), Query Frame = 0

Query: 1   MEILNNFWALLGASIGLYFFVFGFLRNFNELWYFLRLGGKIYNALPPGHMGWPLLGSSLS 60
           MEILNNFWALLGASIGLYFFVFGFLRNFNELWYFLRLGGKIYNALPPGHMGWPLLGSSLS
Sbjct: 1   MEILNNFWALLGASIGLYFFVFGFLRNFNELWYFLRLGGKIYNALPPGHMGWPLLGSSLS 60

Query: 61  FYKAFKIGGDPNSIINHLVSRYGRVRMYKSHLYGSPVIIVTDPEVCRRVYLDDERFKLSY 120
           FYKAFKIGGDPNSIINHLVSRYGRVRMYKSHLYGSPVIIVTDPEVCRRVYLDDERFKLSY
Sbjct: 61  FYKAFKIGGDPNSIINHLVSRYGRVRMYKSHLYGSPVIIVTDPEVCRRVYLDDERFKLSY 120

Query: 121 PKSVNILQGDRSLLKIDYKIVHRLMAAPINGSEALSKYMGFIEEAVVKGLEEWGSVKGPV 180
           PKSVNILQGDRSLLKIDYKIVHRLMAAPINGSEALSKYMGFIEEAVVKGLEEWGSVKGPV
Sbjct: 121 PKSVNILQGDRSLLKIDYKIVHRLMAAPINGSEALSKYMGFIEEAVVKGLEEWGSVKGPV 180

Query: 181 ELLLEFKRLTFKVITGVFLGPAFTDSEIAQLENLYAEIGLAILSIFPYDLPGFSYRRALK 240
           ELLLEFKRLTFKVITGVFLGPAFTDSEIAQLENLYAEIGLAILSIFPYDLPGFSYRRALK
Sbjct: 181 ELLLEFKRLTFKVITGVFLGPAFTDSEIAQLENLYAEIGLAILSIFPYDLPGFSYRRALK 240

Query: 241 ARSKVENIVRPVIERRRRILEENEELEETCQMDAVIKATDKNGEKIFNDNAIFCMLLGLL 300
           ARSKVENIVRPVIERRRRILEENEELEETCQMDAVIKATDKNGEKIFNDNAIFCMLLGLL
Sbjct: 241 ARSKVENIVRPVIERRRRILEENEELEETCQMDAVIKATDKNGEKIFNDNAIFCMLLGLL 300

Query: 301 YAGYHTSAHGAQWTMVHVSENPDVYQKAKEEQEMIIKQRPSTQKGLNFKEIKQMKYATKV 360
           YAGYHTSAHGAQWTMVHVSENPDVYQKAKEEQEMIIKQRPSTQKGLNFKEIKQMKYATKV
Sbjct: 301 YAGYHTSAHGAQWTMVHVSENPDVYQKAKEEQEMIIKQRPSTQKGLNFKEIKQMKYATKV 360

Query: 361 INEMLRLNNIVFTTFRVATTDVNVNGYIIPKGWAVPIWNRGVHMDPEIYQNPLEFDPSRW 420
           INEMLRLNNIVFTTFRVATTDVNVNGYIIPKGWAVPIWNRGVHMDPEIYQNPLEFDPSRW
Sbjct: 361 INEMLRLNNIVFTTFRVATTDVNVNGYIIPKGWAVPIWNRGVHMDPEIYQNPLEFDPSRW 420

Query: 421 DNYVPKPGEFIPFGLGSRYCPGNELARLEICILLHHFLLGYR 463
           DNYVPKPGEFIPFGLGSRYCPGNELARLEICILLHHFLLGYR
Sbjct: 421 DNYVPKPGEFIPFGLGSRYCPGNELARLEICILLHHFLLGYR 462

BLAST of MS005445 vs. NCBI nr
Match: BBI21930.1 (cytochrome P450 monooxygenase [Momordica charantia])

HSP 1 Score: 942.2 bits (2434), Expect = 1.7e-270
Identity = 461/462 (99.78%), Postives = 461/462 (99.78%), Query Frame = 0

Query: 1   MEILNNFWALLGASIGLYFFVFGFLRNFNELWYFLRLGGKIYNALPPGHMGWPLLGSSLS 60
           MEILNNFWALLGASIGLYFFVFGFLRNFNELWYFLRLGGKIYNALPPGHMGWPLLGSSLS
Sbjct: 1   MEILNNFWALLGASIGLYFFVFGFLRNFNELWYFLRLGGKIYNALPPGHMGWPLLGSSLS 60

Query: 61  FYKAFKIGGDPNSIINHLVSRYGRVRMYKSHLYGSPVIIVTDPEVCRRVYLDDERFKLSY 120
           FYKAFKIGGDPNSIINHLVSRYGRVRMYKSHLYGSPVIIVTDPEVCRRVYLDDERFKLSY
Sbjct: 61  FYKAFKIGGDPNSIINHLVSRYGRVRMYKSHLYGSPVIIVTDPEVCRRVYLDDERFKLSY 120

Query: 121 PKSVNILQGDRSLLKIDYKIVHRLMAAPINGSEALSKYMGFIEEAVVKGLEEWGSVKGPV 180
           PKSVNILQGDRSLLKIDYKIVHRLMAAPINGSEALSKYMGFIEEAVVKGLEEWGSVKGPV
Sbjct: 121 PKSVNILQGDRSLLKIDYKIVHRLMAAPINGSEALSKYMGFIEEAVVKGLEEWGSVKGPV 180

Query: 181 ELLLEFKRLTFKVITGVFLGPAFTDSEIAQLENLYAEIGLAILSIFPYDLPGFSYRRALK 240
           ELLLEFKRLTFKVITGVFLGPAFTDSEIAQLENLYAEIGLAILSIFPYDLPGFSYRRALK
Sbjct: 181 ELLLEFKRLTFKVITGVFLGPAFTDSEIAQLENLYAEIGLAILSIFPYDLPGFSYRRALK 240

Query: 241 ARSKVENIVRPVIERRRRILEENEELEETCQMDAVIKATDKNGEKIFNDNAIFCMLLGLL 300
           ARSKVENIVRPVIERRRRILEENEELEETCQMDAVIKATDKNGEKIFNDNAI CMLLGLL
Sbjct: 241 ARSKVENIVRPVIERRRRILEENEELEETCQMDAVIKATDKNGEKIFNDNAIICMLLGLL 300

Query: 301 YAGYHTSAHGAQWTMVHVSENPDVYQKAKEEQEMIIKQRPSTQKGLNFKEIKQMKYATKV 360
           YAGYHTSAHGAQWTMVHVSENPDVYQKAKEEQEMIIKQRPSTQKGLNFKEIKQMKYATKV
Sbjct: 301 YAGYHTSAHGAQWTMVHVSENPDVYQKAKEEQEMIIKQRPSTQKGLNFKEIKQMKYATKV 360

Query: 361 INEMLRLNNIVFTTFRVATTDVNVNGYIIPKGWAVPIWNRGVHMDPEIYQNPLEFDPSRW 420
           INEMLRLNNIVFTTFRVATTDVNVNGYIIPKGWAVPIWNRGVHMDPEIYQNPLEFDPSRW
Sbjct: 361 INEMLRLNNIVFTTFRVATTDVNVNGYIIPKGWAVPIWNRGVHMDPEIYQNPLEFDPSRW 420

Query: 421 DNYVPKPGEFIPFGLGSRYCPGNELARLEICILLHHFLLGYR 463
           DNYVPKPGEFIPFGLGSRYCPGNELARLEICILLHHFLLGYR
Sbjct: 421 DNYVPKPGEFIPFGLGSRYCPGNELARLEICILLHHFLLGYR 462

BLAST of MS005445 vs. NCBI nr
Match: XP_022157141.1 (beta-amyrin 11-oxidase-like isoform X2 [Momordica charantia])

HSP 1 Score: 842.0 bits (2174), Expect = 2.4e-240
Identity = 413/413 (100.00%), Postives = 413/413 (100.00%), Query Frame = 0

Query: 50  MGWPLLGSSLSFYKAFKIGGDPNSIINHLVSRYGRVRMYKSHLYGSPVIIVTDPEVCRRV 109
           MGWPLLGSSLSFYKAFKIGGDPNSIINHLVSRYGRVRMYKSHLYGSPVIIVTDPEVCRRV
Sbjct: 1   MGWPLLGSSLSFYKAFKIGGDPNSIINHLVSRYGRVRMYKSHLYGSPVIIVTDPEVCRRV 60

Query: 110 YLDDERFKLSYPKSVNILQGDRSLLKIDYKIVHRLMAAPINGSEALSKYMGFIEEAVVKG 169
           YLDDERFKLSYPKSVNILQGDRSLLKIDYKIVHRLMAAPINGSEALSKYMGFIEEAVVKG
Sbjct: 61  YLDDERFKLSYPKSVNILQGDRSLLKIDYKIVHRLMAAPINGSEALSKYMGFIEEAVVKG 120

Query: 170 LEEWGSVKGPVELLLEFKRLTFKVITGVFLGPAFTDSEIAQLENLYAEIGLAILSIFPYD 229
           LEEWGSVKGPVELLLEFKRLTFKVITGVFLGPAFTDSEIAQLENLYAEIGLAILSIFPYD
Sbjct: 121 LEEWGSVKGPVELLLEFKRLTFKVITGVFLGPAFTDSEIAQLENLYAEIGLAILSIFPYD 180

Query: 230 LPGFSYRRALKARSKVENIVRPVIERRRRILEENEELEETCQMDAVIKATDKNGEKIFND 289
           LPGFSYRRALKARSKVENIVRPVIERRRRILEENEELEETCQMDAVIKATDKNGEKIFND
Sbjct: 181 LPGFSYRRALKARSKVENIVRPVIERRRRILEENEELEETCQMDAVIKATDKNGEKIFND 240

Query: 290 NAIFCMLLGLLYAGYHTSAHGAQWTMVHVSENPDVYQKAKEEQEMIIKQRPSTQKGLNFK 349
           NAIFCMLLGLLYAGYHTSAHGAQWTMVHVSENPDVYQKAKEEQEMIIKQRPSTQKGLNFK
Sbjct: 241 NAIFCMLLGLLYAGYHTSAHGAQWTMVHVSENPDVYQKAKEEQEMIIKQRPSTQKGLNFK 300

Query: 350 EIKQMKYATKVINEMLRLNNIVFTTFRVATTDVNVNGYIIPKGWAVPIWNRGVHMDPEIY 409
           EIKQMKYATKVINEMLRLNNIVFTTFRVATTDVNVNGYIIPKGWAVPIWNRGVHMDPEIY
Sbjct: 301 EIKQMKYATKVINEMLRLNNIVFTTFRVATTDVNVNGYIIPKGWAVPIWNRGVHMDPEIY 360

Query: 410 QNPLEFDPSRWDNYVPKPGEFIPFGLGSRYCPGNELARLEICILLHHFLLGYR 463
           QNPLEFDPSRWDNYVPKPGEFIPFGLGSRYCPGNELARLEICILLHHFLLGYR
Sbjct: 361 QNPLEFDPSRWDNYVPKPGEFIPFGLGSRYCPGNELARLEICILLHHFLLGYR 413

BLAST of MS005445 vs. NCBI nr
Match: XP_022157153.1 (beta-amyrin 11-oxidase-like [Momordica charantia] >BBI21929.1 cytochrome P450 monooxygenase [Momordica charantia])

HSP 1 Score: 785.4 bits (2027), Expect = 2.7e-223
Identity = 368/462 (79.65%), Postives = 420/462 (90.91%), Query Frame = 0

Query: 1   MEILNNFWALLGASIGLYFFVFGFLRNFNELWYFLRLGGKIYNALPPGHMGWPLLGSSLS 60
           ME+L+NF A+LGA +GLY  VFGFLR FN++WY  +LG  +YNALPPG MGWPL+GSSLS
Sbjct: 1   MELLSNFGAILGALLGLYILVFGFLRKFNDIWYRFKLGKNVYNALPPGDMGWPLVGSSLS 60

Query: 61  FYKAFKIGGDPNSIINHLVSRYGRVRMYKSHLYGSPVIIVTDPEVCRRVYLDDERFKLSY 120
           FY+AFKIGGDPN+++++L+SR+GRVRMYKSHLYG PVIIVTDPEVCRR+YLDDE FKL+Y
Sbjct: 61  FYQAFKIGGDPNAVMDNLISRFGRVRMYKSHLYGFPVIIVTDPEVCRRIYLDDEHFKLNY 120

Query: 121 PKSVNILQGDRSLLKIDYKIVHRLMAAPINGSEALSKYMGFIEEAVVKGLEEWGSVKGPV 180
           PKSV +LQ D SLLK+DYKIVHRLMAAPING EALS+Y+ FIE+AVV+GLEEWGS+KGPV
Sbjct: 121 PKSVKLLQADGSLLKVDYKIVHRLMAAPINGVEALSRYVEFIEDAVVEGLEEWGSMKGPV 180

Query: 181 ELLLEFKRLTFKVITGVFLGPAFTDSEIAQLENLYAEIGLAILSIFPYDLPGFSYRRALK 240
            LL EFKRLTFK+I GVFLGP FTDSE+A+LE+LYAEIG AI+SIFPY+LPGFSYRRALK
Sbjct: 181 PLLHEFKRLTFKIIIGVFLGPVFTDSEVAELESLYAEIGPAIMSIFPYNLPGFSYRRALK 240

Query: 241 ARSKVENIVRPVIERRRRILEENEELEETCQMDAVIKATDKNGEKIFNDNAIFCMLLGLL 300
           AR KVENIVRPVI+ R++ LE N +LEETCQ+DAVIKATDKNGE++FNDNAI CMLLGLL
Sbjct: 241 ARIKVENIVRPVIQNRKKALENNVDLEETCQLDAVIKATDKNGERVFNDNAIICMLLGLL 300

Query: 301 YAGYHTSAHGAQWTMVHVSENPDVYQKAKEEQEMIIKQRPSTQKGLNFKEIKQMKYATKV 360
           YAGYHTSAHGAQWTMVHVSENPDVYQKAKEEQEMIIKQRP  QKGLNFKEIKQMKY  K+
Sbjct: 301 YAGYHTSAHGAQWTMVHVSENPDVYQKAKEEQEMIIKQRPPNQKGLNFKEIKQMKYTAKI 360

Query: 361 INEMLRLNNIVFTTFRVATTDVNVNGYIIPKGWAVPIWNRGVHMDPEIYQNPLEFDPSRW 420
           INE+LRLNNIVFT FR+AT DVNVNGY IPKGW   +W RGVHMD +IY+NPLEF+PSRW
Sbjct: 361 INEVLRLNNIVFTNFRMATKDVNVNGYTIPKGWIAAMWIRGVHMDDKIYENPLEFNPSRW 420

Query: 421 DNYVPKPGEFIPFGLGSRYCPGNELARLEICILLHHFLLGYR 463
           DNY PKPGE+IPFG+GSR+CPGNELA+LE+ +LLHHFLLGYR
Sbjct: 421 DNYTPKPGEYIPFGIGSRFCPGNELAKLEMTVLLHHFLLGYR 462

BLAST of MS005445 vs. NCBI nr
Match: XP_022952147.1 (beta-amyrin 11-oxidase-like [Cucurbita moschata])

HSP 1 Score: 585.1 bits (1507), Expect = 5.3e-163
Identity = 278/462 (60.17%), Postives = 351/462 (75.97%), Query Frame = 0

Query: 1   MEILNNFWALLGASIGLYFFVFGFLRNFNELWYFLRLGGKIYNALPPGHMGWPLLGSSLS 60
           ME++ N   ++GA +G+Y FV    R  NE+WY ++LG K Y +LPPG MGWPLLGSS S
Sbjct: 1   MEMI-NVCTIIGAVLGVYMFV----RKLNEIWYLVKLGRKAYKSLPPGDMGWPLLGSSFS 60

Query: 61  FYKAFKIGGDPNSIINHLVSRYGRVRMYKSHLYGSPVIIVTDPEVCRRVYLDDERFKLSY 120
           FY AF +GGDP+S I HL+SR GRV MYKSHLYG P IIVTDPE+CRR+YLDDE FK +Y
Sbjct: 61  FYNAFTVGGDPDSFIYHLLSRNGRVGMYKSHLYGRPTIIVTDPEICRRIYLDDEIFKPNY 120

Query: 121 PKSVNILQGDRSLLKIDYKIVHRLMAAPINGSEALSKYMGFIEEAVVKGLEEWGSVKGPV 180
           PKSV IL+ +    + D+K  +R+MAAP+NG E LS Y+ FI++ + KGLEEW S+K P+
Sbjct: 121 PKSVKILEVNGFFTRADHKSAYRIMAAPMNGYEVLSHYVDFIDQVMAKGLEEWSSMKEPI 180

Query: 181 ELLLEFKRLTFKVITGVFLGPAFTDSEIAQLENLYAEIGLAILSIFPYDLPGFSYRRALK 240
           ELL E   L FKVI+ +FLG     S +A+LE LY ++G AILSI PYD+PG  Y RALK
Sbjct: 181 ELLGEIGSLFFKVISRIFLGTDIGASRMAELETLYKDLGPAILSILPYDVPGLVYHRALK 240

Query: 241 ARSKVENIVRPVIERRRRILEENEELEETCQMDAVIKATDKNGEKIFNDNAIFCMLLGLL 300
           AR K+EN++  VIE +RR LE+ E  E   QMD +I A D+NG K++++N I  +LLGLL
Sbjct: 241 ARKKIENVLECVIEEKRRALEQKETREVHSQMDKLIMARDENGMKLYDNNTIIDLLLGLL 300

Query: 301 YAGYHTSAHGAQWTMVHVSENPDVYQKAKEEQEMIIKQRPSTQKGLNFKEIKQMKYATKV 360
           +AG+HT A+ A W +VH+SE P ++ KAKEEQE I+KQRPSTQKGL F+EIKQMKY  K 
Sbjct: 301 HAGHHTPAYAAMWALVHISEKPHIFHKAKEEQESILKQRPSTQKGLTFQEIKQMKYLMKF 360

Query: 361 INEMLRLNNIVFTTFRVATTDVNVNGYIIPKGWAVPIWNRGVHMDPEIYQNPLEFDPSRW 420
           INE+LR N IV  +FR A TD+N+N YIIPKGW+V IW R VHMDP++Y NP EFDPSRW
Sbjct: 361 INELLRRNTIVVASFRKAKTDININDYIIPKGWSVLIWTRAVHMDPQLYSNPEEFDPSRW 420

Query: 421 DNYVPKPGEFIPFGLGSRYCPGNELARLEICILLHHFLLGYR 463
           +N+ PKPG FIPFG+G+R+CPG+EL +LEI ILLHHF+L Y+
Sbjct: 421 ENHSPKPGTFIPFGIGTRFCPGSELTKLEITILLHHFILNYK 457

BLAST of MS005445 vs. ExPASy Swiss-Prot
Match: B5BSX1 (Beta-amyrin 11-oxidase OS=Glycyrrhiza uralensis OX=74613 GN=CYP88D6 PE=1 SV=1)

HSP 1 Score: 413.3 bits (1061), Expect = 3.6e-114
Identity = 216/462 (46.75%), Postives = 312/462 (67.53%), Query Frame = 0

Query: 8   WALLGASIGLYFFVFG--FLRNFNELWYFLRLGGKIYNALPPGHMGWPLLGSSLSFYKAF 67
           W  + A+  L  ++FG  F+RN N  +Y ++L  K  + LPPG MGWPL+G  LSF K F
Sbjct: 5   WVCMSAATLLVCYIFGSKFVRNLNGWYYDVKLRRK-EHPLPPGDMGWPLIGDLLSFIKDF 64

Query: 68  KIGGDPNSIINHLVSRYGRVRMYKSHLYGSPVIIVTDPEVCRRVYLDDERFKLSYPKSVN 127
              G P+S IN+LV +YGR  +YK+HL+G+P IIV +P++CRRV  DD  FKL YPKS+ 
Sbjct: 65  S-SGHPDSFINNLVLKYGRSGIYKTHLFGNPSIIVCEPQMCRRVLTDDVNFKLGYPKSIK 124

Query: 128 ILQGDRSLLKI---DYKIVHRLMAAPINGSEALSKYMGFIEEAVVKGLEEWGSVKGPVEL 187
            L   R ++ +   ++++  RL+ +PI G +AL+ Y+  +EE V+  LEE  S+K PVEL
Sbjct: 125 ELARCRPMIDVSNAEHRLFRRLITSPIVGHKALAMYLERLEEIVINSLEELSSMKHPVEL 184

Query: 188 LLEFKRLTFKVITGVFLGPAFTDSEIAQLENLYAEIGLAILSIFPYDLPGFSYRRALKAR 247
           L E K+++FK I  VF+G +  D  I ++ + + ++   + SI P ++PGF++ +AL+AR
Sbjct: 185 LKEMKKVSFKAIVHVFMGSSNQDI-IKKIGSSFTDLYNGMFSI-PINVPGFTFHKALEAR 244

Query: 248 SKVENIVRPVIERRRRILEE--NEELEETCQMDAVIKATDKNGEKIFNDNAIFCMLLGLL 307
            K+  IV+PV++ RR ++E    E  +    +D +++  D+NG K+  D  I  +L+GLL
Sbjct: 245 KKLAKIVQPVVDERRLMIENGPQEGSQRKDLIDILLEVKDENGRKL-EDEDISDLLIGLL 304

Query: 308 YAGYHTSAHGAQWTMVHVSENPDVYQKAKEEQEMIIKQRPSTQKGLNFKEIKQMKYATKV 367
           +AG+ ++A    W++ +++++P + +KAKEEQE I + R S+QK L+ KEIKQM Y ++V
Sbjct: 305 FAGHESTATSLMWSITYLTQHPHILKKAKEEQEEITRTRFSSQKQLSLKEIKQMVYLSQV 364

Query: 368 INEMLRLNNIVFTTFRVATTDVNVNGYIIPKGWAVPIWNRGVHMDPEIYQNPLEFDPSRW 427
           I+E LR  NI F TFR AT DVN+NGYIIPKGW V IW R +HMD E Y NP EF+PSRW
Sbjct: 365 IDETLRCANIAFATFREATADVNINGYIIPKGWRVLIWARAIHMDSEYYPNPEEFNPSRW 424

Query: 428 DNYVPKPGEFIPFGLGSRYCPGNELARLEICILLHHFLLGYR 463
           D+Y  K G F+PFG GSR CPG +LA+LEI I LH+FL  YR
Sbjct: 425 DDYNAKAGTFLPFGAGSRLCPGADLAKLEISIFLHYFLRNYR 461

BLAST of MS005445 vs. ExPASy Swiss-Prot
Match: Q9C5Y2 (Ent-kaurenoic acid oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=KAO2 PE=1 SV=2)

HSP 1 Score: 375.2 bits (962), Expect = 1.1e-102
Identity = 199/449 (44.32%), Postives = 282/449 (62.81%), Query Frame = 0

Query: 17  LYFFVFGFLRNFNELWYFLRLGGKIYNALPPGHMGWPLLGSSLSFYKAFKIGGDPNSIIN 76
           L  FV  ++     +W ++   G+  + LPPG +GWP++G+  SF +AFK   DP S I 
Sbjct: 16  LGLFVLKWVLKRVNVWIYVSKLGEKKHYLPPGDLGWPVIGNMWSFLRAFKT-SDPESFIQ 75

Query: 77  HLVSRYGRVRMYKSHLYGSPVIIVTDPEVCRRVYLDDERFKLSYPKSVNILQGDRSLLKI 136
             ++RYGR  +YK+H++G P ++VT PE CRRV  DD+ F + +PKS   L G +S + I
Sbjct: 76  SYITRYGRTGIYKAHMFGYPCVLVTTPETCRRVLTDDDAFHIGWPKSTMKLIGRKSFVGI 135

Query: 137 ---DYKIVHRLMAAPINGSEALSKYMGFIEEAVVKGLEEWGSVKGPVELLLEFKRLTFKV 196
              ++K + RL +AP+NG EALS Y+ FIEE V   LE+W S  G +E L   ++LTFKV
Sbjct: 136 SFEEHKRLRRLTSAPVNGPEALSVYIQFIEETVNTDLEKW-SKMGEIEFLSHLRKLTFKV 195

Query: 197 ITGVFLGPAFTDSEIAQLENLYAEIGLAILSIFPYDLPGFSYRRALKARSKVENIVRPVI 256
           I  +FL  + ++  +  LE  Y  +   + ++   +LPGF+Y RALKAR K+    + ++
Sbjct: 196 IMYIFLS-SESEHVMDSLEREYTNLNYGVRAM-GINLPGFAYHRALKARKKLVAAFQSIV 255

Query: 257 ERRRRILEENEELEETCQMDAVIKATDKNGEKIFNDNAIFCMLLGLLYAGYHTSAHGAQW 316
             RR   ++N        +D +I   D+NG ++ +D  I  +LL  L AG+ +S H   W
Sbjct: 256 TNRRNQRKQNISSNRKDMLDNLIDVKDENG-RVLDDEEIIDLLLMYLNAGHESSGHLTMW 315

Query: 317 TMVHVSENPDVYQKAKEEQEMIIKQRPSTQKGLNFKEIKQMKYATKVINEMLRLNNIVFT 376
             + + E+P + QKAKEEQE I+K+R   QK L  KE ++M Y ++VI+E LR+     T
Sbjct: 316 ATILMQEHPMILQKAKEEQERIVKKRAPGQK-LTLKETREMVYLSQVIDETLRVITFSLT 375

Query: 377 TFRVATTDVNVNGYIIPKGWAVPIWNRGVHMDPEIYQNPLEFDPSRWDNYVPKPGEFIPF 436
            FR A +DV ++GYIIPKGW V  W R VH+DPEIY +P +FDPSRW+ Y PK G F+PF
Sbjct: 376 AFREAKSDVQMDGYIIPKGWKVLTWFRNVHLDPEIYPDPKKFDPSRWEGYTPKAGTFLPF 435

Query: 437 GLGSRYCPGNELARLEICILLHHFLLGYR 463
           GLGS  CPGN+LA+LEI I LHHFLL YR
Sbjct: 436 GLGSHLCPGNDLAKLEISIFLHHFLLKYR 458

BLAST of MS005445 vs. ExPASy Swiss-Prot
Match: Q43246 (Cytochrome P450 88A1 OS=Zea mays OX=4577 GN=CYP88A1 PE=2 SV=1)

HSP 1 Score: 373.2 bits (957), Expect = 4.1e-102
Identity = 188/435 (43.22%), Postives = 277/435 (63.68%), Query Frame = 0

Query: 32  WY-FLRLGGKIYNALPPGHMGWPLLGSSLSFYKAFKIGGDPNSIINHLVSRYGRVRMYKS 91
           WY    LG      LPPG MGWPL+G   +F +AFK  G P++ I   V R+GR  +Y+S
Sbjct: 58  WYREAALGAARRARLPPGEMGWPLVGGMWAFLRAFK-SGKPDAFIASFVRRFGRTGVYRS 117

Query: 92  HLYGSPVIIVTDPEVCRRVYLDDERFKLSYPKSVNILQGDRSLLKIDY---KIVHRLMAA 151
            ++ SP ++VT  E C++V +DD+ F   +PK+   L G RS + + Y   + + +L AA
Sbjct: 118 FMFSSPTVLVTTAEGCKQVLMDDDAFVTGWPKATVALVGPRSFVAMPYDEHRRIRKLTAA 177

Query: 152 PINGSEALSKYMGFIEEAVVKGLEEWGSVKGPVELLLEFKRLTFKVITGVFLGPAFTDSE 211
           PING +AL+ Y+ FI+  V   L  W    G VE L E +R+TFK+I  +FLG A   + 
Sbjct: 178 PINGFDALTGYLPFIDRTVTSSLRAWADHGGSVEFLTELRRMTFKIIVQIFLGGA-DQAT 237

Query: 212 IAQLENLYAEIGLAILSIFPYDLPGFSYRRALKARSKVENIVRPVIERRRRILEENEELE 271
              LE  Y E+   + ++   +LPGF+YR AL+AR ++  +++ V++ RR    +     
Sbjct: 238 TRALERSYTELNYGMRAM-AINLPGFAYRGALRARRRLVAVLQGVLDERRAARAKGVSGG 297

Query: 272 ETCQMDAVIKATDKNGEKIFNDNAIFCMLLGLLYAGYHTSAHGAQWTMVHVSENPDVYQK 331
               MD +I+A D+ G  + +D+ I  +L+  L AG+ +S H   W  V + ENPD++ +
Sbjct: 298 GVDMMDRLIEAQDERGRHL-DDDEIIDVLVMYLNAGHESSGHITMWATVFLQENPDMFAR 357

Query: 332 AKEEQEMIIKQRPSTQKGLNFKEIKQMKYATKVINEMLRLNNIVFTTFRVATTDVNVNGY 391
           AK EQE I++  PS+Q+GL  ++ ++M+Y ++VI+E LRL NI F +FR AT DV VNGY
Sbjct: 358 AKAEQEAIMRSIPSSQRGLTLRDFRKMEYLSQVIDETLRLVNISFVSFRQATRDVFVNGY 417

Query: 392 IIPKGWAVPIWNRGVHMDPEIYQNPLEFDPSRWDNYVPKPGEFIPFGLGSRYCPGNELAR 451
           +IPKGW V +W R VHMDP++Y +P +FDPSRW+ + P+ G F+ FGLG+R CPGN+LA+
Sbjct: 418 LIPKGWKVQLWYRSVHMDPQVYPDPTKFDPSRWEGHSPRAGTFLAFGLGARLCPGNDLAK 477

Query: 452 LEICILLHHFLLGYR 463
           LEI + LHHFLLGY+
Sbjct: 478 LEISVFLHHFLLGYK 488

BLAST of MS005445 vs. ExPASy Swiss-Prot
Match: Q5VRM7 (Ent-kaurenoic acid oxidase OS=Oryza sativa subsp. japonica OX=39947 GN=KAO PE=2 SV=1)

HSP 1 Score: 371.7 bits (953), Expect = 1.2e-101
Identity = 187/462 (40.48%), Postives = 291/462 (62.99%), Query Frame = 0

Query: 6   NFWALLGASIGLYFFVFGFLRNFNELWYFLRLGGKIYNALPPGHMGWPLLGSSLSFYKAF 65
           + W L  A +     V   +R  ++      LG +  + LPPG MGWP++GS  +F +AF
Sbjct: 16  DLWVLAAAVVAGVVLVDAVVRRAHDWVRVAALGAERRSRLPPGEMGWPMVGSMWAFLRAF 75

Query: 66  KIGGDPNSIINHLVSRYGRVRMYKSHLYGSPVIIVTDPEVCRRVYLDDERFKLSYPKSVN 125
           K  G+P++ I   + R+GR  +Y++ ++ SP I+   PE C++V +DDE F   +PK+  
Sbjct: 76  K-SGNPDAFIASFIRRFGRTGVYRTFMFSSPTILAVTPEACKQVLMDDEGFVTGWPKATV 135

Query: 126 ILQGDRSLLKI---DYKIVHRLMAAPINGSEALSKYMGFIEEAVVKGLEEWGSVK-GPVE 185
            L G +S + +   D++ + +L AAPING +AL+ Y+ FI++ VV  L  W S + G VE
Sbjct: 136 TLIGPKSFVNMSYDDHRRIRKLTAAPINGFDALTTYLSFIDQTVVASLRRWSSPESGQVE 195

Query: 186 LLLEFKRLTFKVITGVFLGPAFTDSEIAQLENLYAEIGLAILSIFPYDLPGFSYRRALKA 245
            L E +R+TFK+I  +F+  A  D+ +  LE  Y ++   + ++   +LPGF+Y RAL+A
Sbjct: 196 FLTELRRMTFKIIVQIFMSGA-DDATMEALERSYTDLNYGMRAM-AINLPGFAYYRALRA 255

Query: 246 RSKVENIVRPVIERRRRILEENEELEETC-QMDAVIKATDKNGEKIFNDNAIFCMLLGLL 305
           R K+ ++++ V++ RR    +  +       MD +I+A D+ G ++ +D  I  +L+  L
Sbjct: 256 RRKLVSVLQGVLDGRRAAAAKGFKRSGAMDMMDRLIEAEDERGRRLADDE-IVDVLIMYL 315

Query: 306 YAGYHTSAHGAQWTMVHVSENPDVYQKAKEEQEMIIKQRPSTQKGLNFKEIKQMKYATKV 365
            AG+ +S H   W  V + ENPD++ +AK EQE I++  P+TQ GL  ++ K+M + ++V
Sbjct: 316 NAGHESSGHITMWATVFLQENPDIFARAKAEQEEIMRSIPATQNGLTLRDFKKMHFLSQV 375

Query: 366 INEMLRLNNIVFTTFRVATTDVNVNGYIIPKGWAVPIWNRGVHMDPEIYQNPLEFDPSRW 425
           ++E LR  NI F +FR AT D+ VNGY+IPKGW V +W R VHMD ++Y +P  F+PSRW
Sbjct: 376 VDETLRCVNISFVSFRQATRDIFVNGYLIPKGWKVQLWYRSVHMDDQVYPDPKMFNPSRW 435

Query: 426 DNYVPKPGEFIPFGLGSRYCPGNELARLEICILLHHFLLGYR 463
           +   PK G F+PFGLG+R CPGN+LA+LEI + LHHFLLGY+
Sbjct: 436 EGPPPKAGTFLPFGLGARLCPGNDLAKLEISVFLHHFLLGYK 473

BLAST of MS005445 vs. ExPASy Swiss-Prot
Match: O23051 (Ent-kaurenoic acid oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=KAO1 PE=1 SV=1)

HSP 1 Score: 364.4 bits (934), Expect = 1.9e-99
Identity = 188/436 (43.12%), Postives = 279/436 (63.99%), Query Frame = 0

Query: 31  LWYFLRLGGKIYNALPPGHMGWPLLGSSLSFYKAFKIGGDPNSIINHLVSRYGRVRMYKS 90
           +W +    G+  + LPPG +GWP +G+ LSF +AFK   DP+S    L+ RYG   +YK+
Sbjct: 31  VWLYESSLGENRHYLPPGDLGWPFIGNMLSFLRAFKT-SDPDSFTRTLIKRYGPKGIYKA 90

Query: 91  HLYGSPVIIVTDPEVCRRVYLDDERFKLSYPKSVNILQGDRSLLKI---DYKIVHRLMAA 150
           H++G+P IIVT  + CRRV  DD+ FK  +P S   L G +S + I   ++K + RL AA
Sbjct: 91  HMFGNPSIIVTTSDTCRRVLTDDDAFKPGWPTSTMELIGRKSFVGISFEEHKRLRRLTAA 150

Query: 151 PINGSEALSKYMGFIEEAVVKGLEEWGSVKGPVELLLEFKRLTFKVITGVFLGPAFTDSE 210
           P+NG EALS Y+ +IEE V+  L++W  + G  E L   ++LTF++I  +FL  + +++ 
Sbjct: 151 PVNGHEALSTYIPYIEENVITVLDKWTKM-GEFEFLTHLRKLTFRIIMYIFLS-SESENV 210

Query: 211 IAQLENLYAEIGLAILSIFPYDLPGFSYRRALKARSKVENIVRPVIERRRRILEENEELE 270
           +  LE  Y  +   + ++   ++PGF+Y RALKAR  +    + ++  RR   ++N    
Sbjct: 211 MDALEREYTALNYGVRAM-AVNIPGFAYHRALKARKTLVAAFQSIVTERRNQRKQNILSN 270

Query: 271 ETCQMDAVIKATDKNGEKIFNDNAIFCMLLGLLYAGYHTSAHGAQWTMVHVSENPDVYQK 330
           +   +D ++   D++G K  +D  I  +LL  L AG+ +S H   W  V + E+P+V Q+
Sbjct: 271 KKDMLDNLLNVKDEDG-KTLDDEEIIDVLLMYLNAGHESSGHTIMWATVFLQEHPEVLQR 330

Query: 331 AKEEQEMIIKQRPSTQKGLNFKEIKQMKYATKVINEMLRLNNIVFTTFRVATTDVNVNGY 390
           AK EQEMI+K RP  QKGL+ KE ++M++ ++V++E LR+     T FR A TDV +NGY
Sbjct: 331 AKAEQEMILKSRPEGQKGLSLKETRKMEFLSQVVDETLRVITFSLTAFREAKTDVEMNGY 390

Query: 391 IIPKGWAVPIWNRGVHMDPEIYQNPLEFDPSRWDN-YVPKPGEFIPFGLGSRYCPGNELA 450
           +IPKGW V  W R VH+DPE++ +P +FDP+RWDN +VPK G F+PFG GS  CPGN+LA
Sbjct: 391 LIPKGWKVLTWFRDVHIDPEVFPDPRKFDPARWDNGFVPKAGAFLPFGAGSHLCPGNDLA 450

Query: 451 RLEICILLHHFLLGYR 463
           +LEI I LHHFLL Y+
Sbjct: 451 KLEISIFLHHFLLKYQ 461

BLAST of MS005445 vs. ExPASy TrEMBL
Match: A0A6J1DVM6 (beta-amyrin 11-oxidase-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111023928 PE=3 SV=1)

HSP 1 Score: 944.5 bits (2440), Expect = 1.7e-271
Identity = 462/462 (100.00%), Postives = 462/462 (100.00%), Query Frame = 0

Query: 1   MEILNNFWALLGASIGLYFFVFGFLRNFNELWYFLRLGGKIYNALPPGHMGWPLLGSSLS 60
           MEILNNFWALLGASIGLYFFVFGFLRNFNELWYFLRLGGKIYNALPPGHMGWPLLGSSLS
Sbjct: 1   MEILNNFWALLGASIGLYFFVFGFLRNFNELWYFLRLGGKIYNALPPGHMGWPLLGSSLS 60

Query: 61  FYKAFKIGGDPNSIINHLVSRYGRVRMYKSHLYGSPVIIVTDPEVCRRVYLDDERFKLSY 120
           FYKAFKIGGDPNSIINHLVSRYGRVRMYKSHLYGSPVIIVTDPEVCRRVYLDDERFKLSY
Sbjct: 61  FYKAFKIGGDPNSIINHLVSRYGRVRMYKSHLYGSPVIIVTDPEVCRRVYLDDERFKLSY 120

Query: 121 PKSVNILQGDRSLLKIDYKIVHRLMAAPINGSEALSKYMGFIEEAVVKGLEEWGSVKGPV 180
           PKSVNILQGDRSLLKIDYKIVHRLMAAPINGSEALSKYMGFIEEAVVKGLEEWGSVKGPV
Sbjct: 121 PKSVNILQGDRSLLKIDYKIVHRLMAAPINGSEALSKYMGFIEEAVVKGLEEWGSVKGPV 180

Query: 181 ELLLEFKRLTFKVITGVFLGPAFTDSEIAQLENLYAEIGLAILSIFPYDLPGFSYRRALK 240
           ELLLEFKRLTFKVITGVFLGPAFTDSEIAQLENLYAEIGLAILSIFPYDLPGFSYRRALK
Sbjct: 181 ELLLEFKRLTFKVITGVFLGPAFTDSEIAQLENLYAEIGLAILSIFPYDLPGFSYRRALK 240

Query: 241 ARSKVENIVRPVIERRRRILEENEELEETCQMDAVIKATDKNGEKIFNDNAIFCMLLGLL 300
           ARSKVENIVRPVIERRRRILEENEELEETCQMDAVIKATDKNGEKIFNDNAIFCMLLGLL
Sbjct: 241 ARSKVENIVRPVIERRRRILEENEELEETCQMDAVIKATDKNGEKIFNDNAIFCMLLGLL 300

Query: 301 YAGYHTSAHGAQWTMVHVSENPDVYQKAKEEQEMIIKQRPSTQKGLNFKEIKQMKYATKV 360
           YAGYHTSAHGAQWTMVHVSENPDVYQKAKEEQEMIIKQRPSTQKGLNFKEIKQMKYATKV
Sbjct: 301 YAGYHTSAHGAQWTMVHVSENPDVYQKAKEEQEMIIKQRPSTQKGLNFKEIKQMKYATKV 360

Query: 361 INEMLRLNNIVFTTFRVATTDVNVNGYIIPKGWAVPIWNRGVHMDPEIYQNPLEFDPSRW 420
           INEMLRLNNIVFTTFRVATTDVNVNGYIIPKGWAVPIWNRGVHMDPEIYQNPLEFDPSRW
Sbjct: 361 INEMLRLNNIVFTTFRVATTDVNVNGYIIPKGWAVPIWNRGVHMDPEIYQNPLEFDPSRW 420

Query: 421 DNYVPKPGEFIPFGLGSRYCPGNELARLEICILLHHFLLGYR 463
           DNYVPKPGEFIPFGLGSRYCPGNELARLEICILLHHFLLGYR
Sbjct: 421 DNYVPKPGEFIPFGLGSRYCPGNELARLEICILLHHFLLGYR 462

BLAST of MS005445 vs. ExPASy TrEMBL
Match: A0A5K7U6M8 (Cytochrome P450 monooxygenase OS=Momordica charantia OX=3673 GN=McCYP88L8 PE=2 SV=1)

HSP 1 Score: 942.2 bits (2434), Expect = 8.2e-271
Identity = 461/462 (99.78%), Postives = 461/462 (99.78%), Query Frame = 0

Query: 1   MEILNNFWALLGASIGLYFFVFGFLRNFNELWYFLRLGGKIYNALPPGHMGWPLLGSSLS 60
           MEILNNFWALLGASIGLYFFVFGFLRNFNELWYFLRLGGKIYNALPPGHMGWPLLGSSLS
Sbjct: 1   MEILNNFWALLGASIGLYFFVFGFLRNFNELWYFLRLGGKIYNALPPGHMGWPLLGSSLS 60

Query: 61  FYKAFKIGGDPNSIINHLVSRYGRVRMYKSHLYGSPVIIVTDPEVCRRVYLDDERFKLSY 120
           FYKAFKIGGDPNSIINHLVSRYGRVRMYKSHLYGSPVIIVTDPEVCRRVYLDDERFKLSY
Sbjct: 61  FYKAFKIGGDPNSIINHLVSRYGRVRMYKSHLYGSPVIIVTDPEVCRRVYLDDERFKLSY 120

Query: 121 PKSVNILQGDRSLLKIDYKIVHRLMAAPINGSEALSKYMGFIEEAVVKGLEEWGSVKGPV 180
           PKSVNILQGDRSLLKIDYKIVHRLMAAPINGSEALSKYMGFIEEAVVKGLEEWGSVKGPV
Sbjct: 121 PKSVNILQGDRSLLKIDYKIVHRLMAAPINGSEALSKYMGFIEEAVVKGLEEWGSVKGPV 180

Query: 181 ELLLEFKRLTFKVITGVFLGPAFTDSEIAQLENLYAEIGLAILSIFPYDLPGFSYRRALK 240
           ELLLEFKRLTFKVITGVFLGPAFTDSEIAQLENLYAEIGLAILSIFPYDLPGFSYRRALK
Sbjct: 181 ELLLEFKRLTFKVITGVFLGPAFTDSEIAQLENLYAEIGLAILSIFPYDLPGFSYRRALK 240

Query: 241 ARSKVENIVRPVIERRRRILEENEELEETCQMDAVIKATDKNGEKIFNDNAIFCMLLGLL 300
           ARSKVENIVRPVIERRRRILEENEELEETCQMDAVIKATDKNGEKIFNDNAI CMLLGLL
Sbjct: 241 ARSKVENIVRPVIERRRRILEENEELEETCQMDAVIKATDKNGEKIFNDNAIICMLLGLL 300

Query: 301 YAGYHTSAHGAQWTMVHVSENPDVYQKAKEEQEMIIKQRPSTQKGLNFKEIKQMKYATKV 360
           YAGYHTSAHGAQWTMVHVSENPDVYQKAKEEQEMIIKQRPSTQKGLNFKEIKQMKYATKV
Sbjct: 301 YAGYHTSAHGAQWTMVHVSENPDVYQKAKEEQEMIIKQRPSTQKGLNFKEIKQMKYATKV 360

Query: 361 INEMLRLNNIVFTTFRVATTDVNVNGYIIPKGWAVPIWNRGVHMDPEIYQNPLEFDPSRW 420
           INEMLRLNNIVFTTFRVATTDVNVNGYIIPKGWAVPIWNRGVHMDPEIYQNPLEFDPSRW
Sbjct: 361 INEMLRLNNIVFTTFRVATTDVNVNGYIIPKGWAVPIWNRGVHMDPEIYQNPLEFDPSRW 420

Query: 421 DNYVPKPGEFIPFGLGSRYCPGNELARLEICILLHHFLLGYR 463
           DNYVPKPGEFIPFGLGSRYCPGNELARLEICILLHHFLLGYR
Sbjct: 421 DNYVPKPGEFIPFGLGSRYCPGNELARLEICILLHHFLLGYR 462

BLAST of MS005445 vs. ExPASy TrEMBL
Match: A0A6J1DX36 (beta-amyrin 11-oxidase-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111023928 PE=3 SV=1)

HSP 1 Score: 842.0 bits (2174), Expect = 1.2e-240
Identity = 413/413 (100.00%), Postives = 413/413 (100.00%), Query Frame = 0

Query: 50  MGWPLLGSSLSFYKAFKIGGDPNSIINHLVSRYGRVRMYKSHLYGSPVIIVTDPEVCRRV 109
           MGWPLLGSSLSFYKAFKIGGDPNSIINHLVSRYGRVRMYKSHLYGSPVIIVTDPEVCRRV
Sbjct: 1   MGWPLLGSSLSFYKAFKIGGDPNSIINHLVSRYGRVRMYKSHLYGSPVIIVTDPEVCRRV 60

Query: 110 YLDDERFKLSYPKSVNILQGDRSLLKIDYKIVHRLMAAPINGSEALSKYMGFIEEAVVKG 169
           YLDDERFKLSYPKSVNILQGDRSLLKIDYKIVHRLMAAPINGSEALSKYMGFIEEAVVKG
Sbjct: 61  YLDDERFKLSYPKSVNILQGDRSLLKIDYKIVHRLMAAPINGSEALSKYMGFIEEAVVKG 120

Query: 170 LEEWGSVKGPVELLLEFKRLTFKVITGVFLGPAFTDSEIAQLENLYAEIGLAILSIFPYD 229
           LEEWGSVKGPVELLLEFKRLTFKVITGVFLGPAFTDSEIAQLENLYAEIGLAILSIFPYD
Sbjct: 121 LEEWGSVKGPVELLLEFKRLTFKVITGVFLGPAFTDSEIAQLENLYAEIGLAILSIFPYD 180

Query: 230 LPGFSYRRALKARSKVENIVRPVIERRRRILEENEELEETCQMDAVIKATDKNGEKIFND 289
           LPGFSYRRALKARSKVENIVRPVIERRRRILEENEELEETCQMDAVIKATDKNGEKIFND
Sbjct: 181 LPGFSYRRALKARSKVENIVRPVIERRRRILEENEELEETCQMDAVIKATDKNGEKIFND 240

Query: 290 NAIFCMLLGLLYAGYHTSAHGAQWTMVHVSENPDVYQKAKEEQEMIIKQRPSTQKGLNFK 349
           NAIFCMLLGLLYAGYHTSAHGAQWTMVHVSENPDVYQKAKEEQEMIIKQRPSTQKGLNFK
Sbjct: 241 NAIFCMLLGLLYAGYHTSAHGAQWTMVHVSENPDVYQKAKEEQEMIIKQRPSTQKGLNFK 300

Query: 350 EIKQMKYATKVINEMLRLNNIVFTTFRVATTDVNVNGYIIPKGWAVPIWNRGVHMDPEIY 409
           EIKQMKYATKVINEMLRLNNIVFTTFRVATTDVNVNGYIIPKGWAVPIWNRGVHMDPEIY
Sbjct: 301 EIKQMKYATKVINEMLRLNNIVFTTFRVATTDVNVNGYIIPKGWAVPIWNRGVHMDPEIY 360

Query: 410 QNPLEFDPSRWDNYVPKPGEFIPFGLGSRYCPGNELARLEICILLHHFLLGYR 463
           QNPLEFDPSRWDNYVPKPGEFIPFGLGSRYCPGNELARLEICILLHHFLLGYR
Sbjct: 361 QNPLEFDPSRWDNYVPKPGEFIPFGLGSRYCPGNELARLEICILLHHFLLGYR 413

BLAST of MS005445 vs. ExPASy TrEMBL
Match: A0A5K7U6A4 (Cytochrome P450 monooxygenase OS=Momordica charantia OX=3673 GN=McCYP88L7 PE=2 SV=1)

HSP 1 Score: 785.4 bits (2027), Expect = 1.3e-223
Identity = 368/462 (79.65%), Postives = 420/462 (90.91%), Query Frame = 0

Query: 1   MEILNNFWALLGASIGLYFFVFGFLRNFNELWYFLRLGGKIYNALPPGHMGWPLLGSSLS 60
           ME+L+NF A+LGA +GLY  VFGFLR FN++WY  +LG  +YNALPPG MGWPL+GSSLS
Sbjct: 1   MELLSNFGAILGALLGLYILVFGFLRKFNDIWYRFKLGKNVYNALPPGDMGWPLVGSSLS 60

Query: 61  FYKAFKIGGDPNSIINHLVSRYGRVRMYKSHLYGSPVIIVTDPEVCRRVYLDDERFKLSY 120
           FY+AFKIGGDPN+++++L+SR+GRVRMYKSHLYG PVIIVTDPEVCRR+YLDDE FKL+Y
Sbjct: 61  FYQAFKIGGDPNAVMDNLISRFGRVRMYKSHLYGFPVIIVTDPEVCRRIYLDDEHFKLNY 120

Query: 121 PKSVNILQGDRSLLKIDYKIVHRLMAAPINGSEALSKYMGFIEEAVVKGLEEWGSVKGPV 180
           PKSV +LQ D SLLK+DYKIVHRLMAAPING EALS+Y+ FIE+AVV+GLEEWGS+KGPV
Sbjct: 121 PKSVKLLQADGSLLKVDYKIVHRLMAAPINGVEALSRYVEFIEDAVVEGLEEWGSMKGPV 180

Query: 181 ELLLEFKRLTFKVITGVFLGPAFTDSEIAQLENLYAEIGLAILSIFPYDLPGFSYRRALK 240
            LL EFKRLTFK+I GVFLGP FTDSE+A+LE+LYAEIG AI+SIFPY+LPGFSYRRALK
Sbjct: 181 PLLHEFKRLTFKIIIGVFLGPVFTDSEVAELESLYAEIGPAIMSIFPYNLPGFSYRRALK 240

Query: 241 ARSKVENIVRPVIERRRRILEENEELEETCQMDAVIKATDKNGEKIFNDNAIFCMLLGLL 300
           AR KVENIVRPVI+ R++ LE N +LEETCQ+DAVIKATDKNGE++FNDNAI CMLLGLL
Sbjct: 241 ARIKVENIVRPVIQNRKKALENNVDLEETCQLDAVIKATDKNGERVFNDNAIICMLLGLL 300

Query: 301 YAGYHTSAHGAQWTMVHVSENPDVYQKAKEEQEMIIKQRPSTQKGLNFKEIKQMKYATKV 360
           YAGYHTSAHGAQWTMVHVSENPDVYQKAKEEQEMIIKQRP  QKGLNFKEIKQMKY  K+
Sbjct: 301 YAGYHTSAHGAQWTMVHVSENPDVYQKAKEEQEMIIKQRPPNQKGLNFKEIKQMKYTAKI 360

Query: 361 INEMLRLNNIVFTTFRVATTDVNVNGYIIPKGWAVPIWNRGVHMDPEIYQNPLEFDPSRW 420
           INE+LRLNNIVFT FR+AT DVNVNGY IPKGW   +W RGVHMD +IY+NPLEF+PSRW
Sbjct: 361 INEVLRLNNIVFTNFRMATKDVNVNGYTIPKGWIAAMWIRGVHMDDKIYENPLEFNPSRW 420

Query: 421 DNYVPKPGEFIPFGLGSRYCPGNELARLEICILLHHFLLGYR 463
           DNY PKPGE+IPFG+GSR+CPGNELA+LE+ +LLHHFLLGYR
Sbjct: 421 DNYTPKPGEYIPFGIGSRFCPGNELAKLEMTVLLHHFLLGYR 462

BLAST of MS005445 vs. ExPASy TrEMBL
Match: A0A6J1GJM1 (beta-amyrin 11-oxidase-like OS=Cucurbita moschata OX=3662 GN=LOC111454910 PE=3 SV=1)

HSP 1 Score: 585.1 bits (1507), Expect = 2.6e-163
Identity = 278/462 (60.17%), Postives = 351/462 (75.97%), Query Frame = 0

Query: 1   MEILNNFWALLGASIGLYFFVFGFLRNFNELWYFLRLGGKIYNALPPGHMGWPLLGSSLS 60
           ME++ N   ++GA +G+Y FV    R  NE+WY ++LG K Y +LPPG MGWPLLGSS S
Sbjct: 1   MEMI-NVCTIIGAVLGVYMFV----RKLNEIWYLVKLGRKAYKSLPPGDMGWPLLGSSFS 60

Query: 61  FYKAFKIGGDPNSIINHLVSRYGRVRMYKSHLYGSPVIIVTDPEVCRRVYLDDERFKLSY 120
           FY AF +GGDP+S I HL+SR GRV MYKSHLYG P IIVTDPE+CRR+YLDDE FK +Y
Sbjct: 61  FYNAFTVGGDPDSFIYHLLSRNGRVGMYKSHLYGRPTIIVTDPEICRRIYLDDEIFKPNY 120

Query: 121 PKSVNILQGDRSLLKIDYKIVHRLMAAPINGSEALSKYMGFIEEAVVKGLEEWGSVKGPV 180
           PKSV IL+ +    + D+K  +R+MAAP+NG E LS Y+ FI++ + KGLEEW S+K P+
Sbjct: 121 PKSVKILEVNGFFTRADHKSAYRIMAAPMNGYEVLSHYVDFIDQVMAKGLEEWSSMKEPI 180

Query: 181 ELLLEFKRLTFKVITGVFLGPAFTDSEIAQLENLYAEIGLAILSIFPYDLPGFSYRRALK 240
           ELL E   L FKVI+ +FLG     S +A+LE LY ++G AILSI PYD+PG  Y RALK
Sbjct: 181 ELLGEIGSLFFKVISRIFLGTDIGASRMAELETLYKDLGPAILSILPYDVPGLVYHRALK 240

Query: 241 ARSKVENIVRPVIERRRRILEENEELEETCQMDAVIKATDKNGEKIFNDNAIFCMLLGLL 300
           AR K+EN++  VIE +RR LE+ E  E   QMD +I A D+NG K++++N I  +LLGLL
Sbjct: 241 ARKKIENVLECVIEEKRRALEQKETREVHSQMDKLIMARDENGMKLYDNNTIIDLLLGLL 300

Query: 301 YAGYHTSAHGAQWTMVHVSENPDVYQKAKEEQEMIIKQRPSTQKGLNFKEIKQMKYATKV 360
           +AG+HT A+ A W +VH+SE P ++ KAKEEQE I+KQRPSTQKGL F+EIKQMKY  K 
Sbjct: 301 HAGHHTPAYAAMWALVHISEKPHIFHKAKEEQESILKQRPSTQKGLTFQEIKQMKYLMKF 360

Query: 361 INEMLRLNNIVFTTFRVATTDVNVNGYIIPKGWAVPIWNRGVHMDPEIYQNPLEFDPSRW 420
           INE+LR N IV  +FR A TD+N+N YIIPKGW+V IW R VHMDP++Y NP EFDPSRW
Sbjct: 361 INELLRRNTIVVASFRKAKTDININDYIIPKGWSVLIWTRAVHMDPQLYSNPEEFDPSRW 420

Query: 421 DNYVPKPGEFIPFGLGSRYCPGNELARLEICILLHHFLLGYR 463
           +N+ PKPG FIPFG+G+R+CPG+EL +LEI ILLHHF+L Y+
Sbjct: 421 ENHSPKPGTFIPFGIGTRFCPGSELTKLEITILLHHFILNYK 457

BLAST of MS005445 vs. TAIR 10
Match: AT2G32440.1 (ent-kaurenoic acid hydroxylase 2 )

HSP 1 Score: 375.2 bits (962), Expect = 7.7e-104
Identity = 199/449 (44.32%), Postives = 282/449 (62.81%), Query Frame = 0

Query: 17  LYFFVFGFLRNFNELWYFLRLGGKIYNALPPGHMGWPLLGSSLSFYKAFKIGGDPNSIIN 76
           L  FV  ++     +W ++   G+  + LPPG +GWP++G+  SF +AFK   DP S I 
Sbjct: 16  LGLFVLKWVLKRVNVWIYVSKLGEKKHYLPPGDLGWPVIGNMWSFLRAFKT-SDPESFIQ 75

Query: 77  HLVSRYGRVRMYKSHLYGSPVIIVTDPEVCRRVYLDDERFKLSYPKSVNILQGDRSLLKI 136
             ++RYGR  +YK+H++G P ++VT PE CRRV  DD+ F + +PKS   L G +S + I
Sbjct: 76  SYITRYGRTGIYKAHMFGYPCVLVTTPETCRRVLTDDDAFHIGWPKSTMKLIGRKSFVGI 135

Query: 137 ---DYKIVHRLMAAPINGSEALSKYMGFIEEAVVKGLEEWGSVKGPVELLLEFKRLTFKV 196
              ++K + RL +AP+NG EALS Y+ FIEE V   LE+W S  G +E L   ++LTFKV
Sbjct: 136 SFEEHKRLRRLTSAPVNGPEALSVYIQFIEETVNTDLEKW-SKMGEIEFLSHLRKLTFKV 195

Query: 197 ITGVFLGPAFTDSEIAQLENLYAEIGLAILSIFPYDLPGFSYRRALKARSKVENIVRPVI 256
           I  +FL  + ++  +  LE  Y  +   + ++   +LPGF+Y RALKAR K+    + ++
Sbjct: 196 IMYIFLS-SESEHVMDSLEREYTNLNYGVRAM-GINLPGFAYHRALKARKKLVAAFQSIV 255

Query: 257 ERRRRILEENEELEETCQMDAVIKATDKNGEKIFNDNAIFCMLLGLLYAGYHTSAHGAQW 316
             RR   ++N        +D +I   D+NG ++ +D  I  +LL  L AG+ +S H   W
Sbjct: 256 TNRRNQRKQNISSNRKDMLDNLIDVKDENG-RVLDDEEIIDLLLMYLNAGHESSGHLTMW 315

Query: 317 TMVHVSENPDVYQKAKEEQEMIIKQRPSTQKGLNFKEIKQMKYATKVINEMLRLNNIVFT 376
             + + E+P + QKAKEEQE I+K+R   QK L  KE ++M Y ++VI+E LR+     T
Sbjct: 316 ATILMQEHPMILQKAKEEQERIVKKRAPGQK-LTLKETREMVYLSQVIDETLRVITFSLT 375

Query: 377 TFRVATTDVNVNGYIIPKGWAVPIWNRGVHMDPEIYQNPLEFDPSRWDNYVPKPGEFIPF 436
            FR A +DV ++GYIIPKGW V  W R VH+DPEIY +P +FDPSRW+ Y PK G F+PF
Sbjct: 376 AFREAKSDVQMDGYIIPKGWKVLTWFRNVHLDPEIYPDPKKFDPSRWEGYTPKAGTFLPF 435

Query: 437 GLGSRYCPGNELARLEICILLHHFLLGYR 463
           GLGS  CPGN+LA+LEI I LHHFLL YR
Sbjct: 436 GLGSHLCPGNDLAKLEISIFLHHFLLKYR 458

BLAST of MS005445 vs. TAIR 10
Match: AT2G32440.2 (ent-kaurenoic acid hydroxylase 2 )

HSP 1 Score: 375.2 bits (962), Expect = 7.7e-104
Identity = 199/449 (44.32%), Postives = 282/449 (62.81%), Query Frame = 0

Query: 17  LYFFVFGFLRNFNELWYFLRLGGKIYNALPPGHMGWPLLGSSLSFYKAFKIGGDPNSIIN 76
           L  FV  ++     +W ++   G+  + LPPG +GWP++G+  SF +AFK   DP S I 
Sbjct: 16  LGLFVLKWVLKRVNVWIYVSKLGEKKHYLPPGDLGWPVIGNMWSFLRAFKT-SDPESFIQ 75

Query: 77  HLVSRYGRVRMYKSHLYGSPVIIVTDPEVCRRVYLDDERFKLSYPKSVNILQGDRSLLKI 136
             ++RYGR  +YK+H++G P ++VT PE CRRV  DD+ F + +PKS   L G +S + I
Sbjct: 76  SYITRYGRTGIYKAHMFGYPCVLVTTPETCRRVLTDDDAFHIGWPKSTMKLIGRKSFVGI 135

Query: 137 ---DYKIVHRLMAAPINGSEALSKYMGFIEEAVVKGLEEWGSVKGPVELLLEFKRLTFKV 196
              ++K + RL +AP+NG EALS Y+ FIEE V   LE+W S  G +E L   ++LTFKV
Sbjct: 136 SFEEHKRLRRLTSAPVNGPEALSVYIQFIEETVNTDLEKW-SKMGEIEFLSHLRKLTFKV 195

Query: 197 ITGVFLGPAFTDSEIAQLENLYAEIGLAILSIFPYDLPGFSYRRALKARSKVENIVRPVI 256
           I  +FL  + ++  +  LE  Y  +   + ++   +LPGF+Y RALKAR K+    + ++
Sbjct: 196 IMYIFLS-SESEHVMDSLEREYTNLNYGVRAM-GINLPGFAYHRALKARKKLVAAFQSIV 255

Query: 257 ERRRRILEENEELEETCQMDAVIKATDKNGEKIFNDNAIFCMLLGLLYAGYHTSAHGAQW 316
             RR   ++N        +D +I   D+NG ++ +D  I  +LL  L AG+ +S H   W
Sbjct: 256 TNRRNQRKQNISSNRKDMLDNLIDVKDENG-RVLDDEEIIDLLLMYLNAGHESSGHLTMW 315

Query: 317 TMVHVSENPDVYQKAKEEQEMIIKQRPSTQKGLNFKEIKQMKYATKVINEMLRLNNIVFT 376
             + + E+P + QKAKEEQE I+K+R   QK L  KE ++M Y ++VI+E LR+     T
Sbjct: 316 ATILMQEHPMILQKAKEEQERIVKKRAPGQK-LTLKETREMVYLSQVIDETLRVITFSLT 375

Query: 377 TFRVATTDVNVNGYIIPKGWAVPIWNRGVHMDPEIYQNPLEFDPSRWDNYVPKPGEFIPF 436
            FR A +DV ++GYIIPKGW V  W R VH+DPEIY +P +FDPSRW+ Y PK G F+PF
Sbjct: 376 AFREAKSDVQMDGYIIPKGWKVLTWFRNVHLDPEIYPDPKKFDPSRWEGYTPKAGTFLPF 435

Query: 437 GLGSRYCPGNELARLEICILLHHFLLGYR 463
           GLGS  CPGN+LA+LEI I LHHFLL YR
Sbjct: 436 GLGSHLCPGNDLAKLEISIFLHHFLLKYR 458

BLAST of MS005445 vs. TAIR 10
Match: AT1G05160.1 (cytochrome P450, family 88, subfamily A, polypeptide 3 )

HSP 1 Score: 364.4 bits (934), Expect = 1.4e-100
Identity = 188/436 (43.12%), Postives = 279/436 (63.99%), Query Frame = 0

Query: 31  LWYFLRLGGKIYNALPPGHMGWPLLGSSLSFYKAFKIGGDPNSIINHLVSRYGRVRMYKS 90
           +W +    G+  + LPPG +GWP +G+ LSF +AFK   DP+S    L+ RYG   +YK+
Sbjct: 31  VWLYESSLGENRHYLPPGDLGWPFIGNMLSFLRAFKT-SDPDSFTRTLIKRYGPKGIYKA 90

Query: 91  HLYGSPVIIVTDPEVCRRVYLDDERFKLSYPKSVNILQGDRSLLKI---DYKIVHRLMAA 150
           H++G+P IIVT  + CRRV  DD+ FK  +P S   L G +S + I   ++K + RL AA
Sbjct: 91  HMFGNPSIIVTTSDTCRRVLTDDDAFKPGWPTSTMELIGRKSFVGISFEEHKRLRRLTAA 150

Query: 151 PINGSEALSKYMGFIEEAVVKGLEEWGSVKGPVELLLEFKRLTFKVITGVFLGPAFTDSE 210
           P+NG EALS Y+ +IEE V+  L++W  + G  E L   ++LTF++I  +FL  + +++ 
Sbjct: 151 PVNGHEALSTYIPYIEENVITVLDKWTKM-GEFEFLTHLRKLTFRIIMYIFLS-SESENV 210

Query: 211 IAQLENLYAEIGLAILSIFPYDLPGFSYRRALKARSKVENIVRPVIERRRRILEENEELE 270
           +  LE  Y  +   + ++   ++PGF+Y RALKAR  +    + ++  RR   ++N    
Sbjct: 211 MDALEREYTALNYGVRAM-AVNIPGFAYHRALKARKTLVAAFQSIVTERRNQRKQNILSN 270

Query: 271 ETCQMDAVIKATDKNGEKIFNDNAIFCMLLGLLYAGYHTSAHGAQWTMVHVSENPDVYQK 330
           +   +D ++   D++G K  +D  I  +LL  L AG+ +S H   W  V + E+P+V Q+
Sbjct: 271 KKDMLDNLLNVKDEDG-KTLDDEEIIDVLLMYLNAGHESSGHTIMWATVFLQEHPEVLQR 330

Query: 331 AKEEQEMIIKQRPSTQKGLNFKEIKQMKYATKVINEMLRLNNIVFTTFRVATTDVNVNGY 390
           AK EQEMI+K RP  QKGL+ KE ++M++ ++V++E LR+     T FR A TDV +NGY
Sbjct: 331 AKAEQEMILKSRPEGQKGLSLKETRKMEFLSQVVDETLRVITFSLTAFREAKTDVEMNGY 390

Query: 391 IIPKGWAVPIWNRGVHMDPEIYQNPLEFDPSRWDN-YVPKPGEFIPFGLGSRYCPGNELA 450
           +IPKGW V  W R VH+DPE++ +P +FDP+RWDN +VPK G F+PFG GS  CPGN+LA
Sbjct: 391 LIPKGWKVLTWFRDVHIDPEVFPDPRKFDPARWDNGFVPKAGAFLPFGAGSHLCPGNDLA 450

Query: 451 RLEICILLHHFLLGYR 463
           +LEI I LHHFLL Y+
Sbjct: 451 KLEISIFLHHFLLKYQ 461

BLAST of MS005445 vs. TAIR 10
Match: AT1G12740.1 (cytochrome P450, family 87, subfamily A, polypeptide 2 )

HSP 1 Score: 209.9 bits (533), Expect = 4.3e-54
Identity = 140/439 (31.89%), Postives = 218/439 (49.66%), Query Frame = 0

Query: 32  WYFLRLGGKIYNALPPGHMGWPLLGSSLSFYKAFKIGGDPNSIINHLVSRYGRVRMYKSH 91
           W +     K    LPPG MG+PLLG S+ F+K  K    P   I   V +YG +  +K++
Sbjct: 18  WVYSWRNPKCRGKLPPGSMGFPLLGESIQFFKPNKTSDIP-PFIKERVKKYGPI--FKTN 77

Query: 92  LYGSPVIIVTDPEVCRRVYLDDER-FKLSYPKSVNILQGDR---SLLKIDYKIVHRLMAA 151
           L G PVI+ TD ++   V+  + R F+  YP +   + G +   SL    YK + + M  
Sbjct: 78  LVGRPVIVSTDADLSYFVFNQEGRCFQSWYPDTFTHIFGKKNVGSLHGFMYKYL-KNMVL 137

Query: 152 PINGSEALSKYMGFIEEAVVKGLEEWGSVKGPVELLLEFKRLTFKVITGVFLGPAFTDSE 211
            + G + L K +  +E    K LE W +        +E K  T  +I  +      +   
Sbjct: 138 TLFGHDGLKKMLPQVEMTANKRLELWSNQDS-----VELKDATASMIFDLTAKKLISHDP 197

Query: 212 IAQLENLYAEIGLAI--LSIFPYDLPGFSYRRALKARSKVENIVRPVIERRRRILEENEE 271
               ENL A     I  L  FP+D+PG +Y + L+ R+K   ++R +++ RR    EN  
Sbjct: 198 DKSSENLRANFVAFIQGLISFPFDIPGTAYHKCLQGRAKAMKMLRNMLQERR----ENPR 257

Query: 272 LEETCQMDAVIKATDKNGEKIFNDNAIFCMLLGLLYAGYHTSAHGAQWTMVHVSENPDVY 331
              +   D VI+   K G  +  + A+  M + LL+A + T++      +  +S++P+V 
Sbjct: 258 KNPSDFFDYVIEEIQKEGTILTEEIALDLMFV-LLFASFETTSLALTLAIKFLSDDPEVL 317

Query: 332 QKAKEEQEMIIKQRPSTQKGLNFKEIKQMKYATKVINEMLRLNNIVFTTFRVATTDVNVN 391
           ++  EE E I++ R     GL ++E K M Y  + INE  RL NIV   FR A  D+   
Sbjct: 318 KRLTEEHETILRNREDADSGLTWEEYKSMTYTFQFINETARLANIVPAIFRKALRDIKFK 377

Query: 392 GYIIPKGWAVPIWNRGVHMDPEIYQNPLEFDPSRWD--NYVPKPGEFIPFGLGSRYCPGN 451
            Y IP GWAV +    VH++PE+Y++PL F+PSRW+          F+ FG G R+C G 
Sbjct: 378 DYTIPAGWAVMVCPPAVHLNPEMYKDPLVFNPSRWEGSKVTNASKHFMAFGGGMRFCVGT 437

Query: 452 ELARLEICILLHHFLLGYR 463
           +  +L++   LH  +  YR
Sbjct: 438 DFTKLQMAAFLHSLVTKYR 442

BLAST of MS005445 vs. TAIR 10
Match: AT3G19270.1 (cytochrome P450, family 707, subfamily A, polypeptide 4 )

HSP 1 Score: 209.1 bits (531), Expect = 7.3e-54
Identity = 139/429 (32.40%), Postives = 213/429 (49.65%), Query Frame = 0

Query: 45  LPPGHMGWPLLGSSLSFYKAFKIGGDPNSIINHLVSRYGRVRMYKSHLYGSPVIIVTDPE 104
           LPPG MGWP LG +L  Y       +PN        RYG +  +K+ + G P +++  PE
Sbjct: 34  LPPGSMGWPYLGETLQLYSQ-----NPNVFFTSKQKRYGEI--FKTRILGYPCVMLASPE 93

Query: 105 VCRRVYLDD-ERFKLSYPKSVNILQGDRSLL--KIDYKIVHRLMAAPINGSEALSKYMGF 164
             R V +     FK +YP+S   L G  +L   + DY    R +       E + K +  
Sbjct: 94  AARFVLVTHAHMFKPTYPRSKEKLIGPSALFFHQGDYHSHIRKLVQSSFYPETIRKLIPD 153

Query: 165 IEEAVVKGLEEWGSVKGPVELLLEFKRLTFKV-ITGVFLGPAFTDSEIAQLENLYAEIGL 224
           IE   +  L+ W ++   V    E K+  F V I  +F            LE+ Y EI  
Sbjct: 154 IEHIALSSLQSWANMP-IVSTYQEMKKFAFDVGILAIF----------GHLESSYKEILK 213

Query: 225 AILSI-------FPYDLPGFSYRRALKARSKVENIVRPVIERRRRILEENEELEETCQMD 284
              +I       FP  LPG SY +AL AR +++ IV  +I  RR      +   +T  + 
Sbjct: 214 HNYNIVDKGYNSFPMSLPGTSYHKALMARKQLKTIVSEIICERR-----EKRALQTDFLG 273

Query: 285 AVIKATDKNGEKIFNDNAIFCMLLGLLYAGYHTSAHGAQWTMVHVSENPDVYQKAKEEQE 344
            ++   ++ G ++     I   ++G+L+A   T+A    W + ++ ++  + +  K EQ+
Sbjct: 274 HLLNFKNEKG-RVLTQEQIADNIIGVLFAAQDTTASCLTWILKYLHDDQKLLEAVKAEQK 333

Query: 345 MIIKQRPSTQKGLNFKEIKQMKYATKVINEMLRLNNIVFTTFRVATTDVNVNGYIIPKGW 404
            I ++    +K L +++ + M    KVI E LR+ +I+  TFR A  DV   GY+IPKGW
Sbjct: 334 AIYEENSREKKPLTWRQTRNMPLTHKVIVESLRMASIISFTFREAVVDVEYKGYLIPKGW 393

Query: 405 AVPIWNRGVHMDPEIYQNPLEFDPSRWDNYVPKPGEFIPFGLGSRYCPGNELARLEICIL 463
            V    R +H +P+ + NP  FDPSR++   PKP  F+PFG G   CPGNELA+L+I I 
Sbjct: 394 KVMPLFRNIHHNPKYFSNPEVFDPSRFE-VNPKPNTFMPFGSGVHACPGNELAKLQILIF 437