MS005443 (gene) Bitter gourd (TR) v1

BLAST of MS005443 vs. NCBI nr
Match: XP_022157153.1 (beta-amyrin 11-oxidase-like [Momordica charantia] >BBI21929.1 cytochrome P450 monooxygenase [Momordica charantia])

HSP 1 Score: 832.0 bits (2148), Expect = 2.3e-237
Identity = 407/438 (92.92%), Postives = 421/438 (96.12%), Query Frame = 0

Query: 1   MRKFNDLWYRFRLGKNVYNALPPGDMGWPLVGSSLSFYQSFKIGGDPNSVMDNLISRFGR 60
           +RKFND+WYRF+LGKNVYNALPPGDMGWPLVGSSLSFYQ+FKIGGDPN+VMDNLISRFGR
Sbjct: 25  LRKFNDIWYRFKLGKNVYNALPPGDMGWPLVGSSLSFYQAFKIGGDPNAVMDNLISRFGR 84

Query: 61  VRMYKSHLYGFPVIIVTDPEVCRRIYLDDEHFKLNYPKSVKLLQADGSLLKVDYKIVHRL 120
           VRMYKSHLYGFPVIIVTDPEVCRRIYLDDEHFKLNYPKSVKLLQADGSLLKVDYKIVHRL
Sbjct: 85  VRMYKSHLYGFPVIIVTDPEVCRRIYLDDEHFKLNYPKSVKLLQADGSLLKVDYKIVHRL 144

Query: 121 MAAPINGVEALSRYVEFIEDAVVEGLEEWGSMKGPVPLLHEFKRLTFKIIIGVFLGPVFT 180
           MAAPINGVEALSRYVEFIEDAVVEGLEEWGSMKGPVPLLHEFKRLTFKIIIGVFLGPVFT
Sbjct: 145 MAAPINGVEALSRYVEFIEDAVVEGLEEWGSMKGPVPLLHEFKRLTFKIIIGVFLGPVFT 204

Query: 181 DSEVAELESLYAEIGPAIMSIFPYNLPGFSYRRALKARIKVEDIVRPVIQKRKKALENNV 240
           DSEVAELESLYAEIGPAIMSIFPYNLPGFSYRRALKARIKVE+IVRPVIQ RKKALENNV
Sbjct: 205 DSEVAELESLYAEIGPAIMSIFPYNLPGFSYRRALKARIKVENIVRPVIQNRKKALENNV 264

Query: 241 DLEETCQLDAVIKATDKNGEKIFNDNAIICMLLGLLYAGYHTSAHGAQWTMVHVSENPDV 300
           DLEETCQLDAVIKATDKNGE++FNDNAIICMLLGLLYAGYHTSAHGAQWTMVHVSENPDV
Sbjct: 265 DLEETCQLDAVIKATDKNGERVFNDNAIICMLLGLLYAGYHTSAHGAQWTMVHVSENPDV 324

Query: 301 YQKAKEEQEMIIKQRPPTQKGLNFKEIKQMKYTAKIELIIYSL-IIIFFNYCFLS-SLLI 360
           YQKAKEEQEMIIKQRPP QKGLNFKEIKQMKYTAKI   +  L  I+F N+   +  + +
Sbjct: 325 YQKAKEEQEMIIKQRPPNQKGLNFKEIKQMKYTAKIINEVLRLNNIVFTNFRMATKDVNV 384

Query: 361 IGYTIPKGWIAAMWIRGVHMDDKIYENPLEFNPSRWDNYTPKPGEYIPFGIGSRFCPGNE 420
            GYTIPKGWIAAMWIRGVHMDDKIYENPLEFNPSRWDNYTPKPGEYIPFGIGSRFCPGNE
Sbjct: 385 NGYTIPKGWIAAMWIRGVHMDDKIYENPLEFNPSRWDNYTPKPGEYIPFGIGSRFCPGNE 444

Query: 421 LAKLEMTVLLHHFLLGYR 437
           LAKLEMTVLLHHFLLGYR
Sbjct: 445 LAKLEMTVLLHHFLLGYR 462

BLAST of MS005443 vs. NCBI nr
Match: BBI21930.1 (cytochrome P450 monooxygenase [Momordica charantia])

HSP 1 Score: 711.4 bits (1835), Expect = 4.6e-201
Identity = 337/438 (76.94%), Postives = 389/438 (88.81%), Query Frame = 0

Query: 1   MRKFNDLWYRFRLGKNVYNALPPGDMGWPLVGSSLSFYQSFKIGGDPNSVMDNLISRFGR 60
           +R FN+LWY  RLG  +YNALPPG MGWPL+GSSLSFY++FKIGGDPNS++++L+SR+GR
Sbjct: 25  LRNFNELWYFLRLGGKIYNALPPGHMGWPLLGSSLSFYKAFKIGGDPNSIINHLVSRYGR 84

Query: 61  VRMYKSHLYGFPVIIVTDPEVCRRIYLDDEHFKLNYPKSVKLLQADGSLLKVDYKIVHRL 120
           VRMYKSHLYG PVIIVTDPEVCRR+YLDDE FKL+YPKSV +LQ D SLLK+DYKIVHRL
Sbjct: 85  VRMYKSHLYGSPVIIVTDPEVCRRVYLDDERFKLSYPKSVNILQGDRSLLKIDYKIVHRL 144

Query: 121 MAAPINGVEALSRYVEFIEDAVVEGLEEWGSMKGPVPLLHEFKRLTFKIIIGVFLGPVFT 180
           MAAPING EALS+Y+ FIE+AVV+GLEEWGS+KGPV LL EFKRLTFK+I GVFLGP FT
Sbjct: 145 MAAPINGSEALSKYMGFIEEAVVKGLEEWGSVKGPVELLLEFKRLTFKVITGVFLGPAFT 204

Query: 181 DSEVAELESLYAEIGPAIMSIFPYNLPGFSYRRALKARIKVEDIVRPVIQKRKKALENNV 240
           DSE+A+LE+LYAEIG AI+SIFPY+LPGFSYRRALKAR KVE+IVRPVI++R++ LE N 
Sbjct: 205 DSEIAQLENLYAEIGLAILSIFPYDLPGFSYRRALKARSKVENIVRPVIERRRRILEENE 264

Query: 241 DLEETCQLDAVIKATDKNGEKIFNDNAIICMLLGLLYAGYHTSAHGAQWTMVHVSENPDV 300
           +LEETCQ+DAVIKATDKNGEKIFNDNAIICMLLGLLYAGYHTSAHGAQWTMVHVSENPDV
Sbjct: 265 ELEETCQMDAVIKATDKNGEKIFNDNAIICMLLGLLYAGYHTSAHGAQWTMVHVSENPDV 324

Query: 301 YQKAKEEQEMIIKQRPPTQKGLNFKEIKQMKYTAKI--ELIIYSLIIIFFNYCFLSSLLI 360
           YQKAKEEQEMIIKQRP TQKGLNFKEIKQMKY  K+  E++  + I+        + + +
Sbjct: 325 YQKAKEEQEMIIKQRPSTQKGLNFKEIKQMKYATKVINEMLRLNNIVFTTFRVATTDVNV 384

Query: 361 IGYTIPKGWIAAMWIRGVHMDDKIYENPLEFNPSRWDNYTPKPGEYIPFGIGSRFCPGNE 420
            GY IPKGW   +W RGVHMD +IY+NPLEF+PSRWDNY PKPGE+IPFG+GSR+CPGNE
Sbjct: 385 NGYIIPKGWAVPIWNRGVHMDPEIYQNPLEFDPSRWDNYVPKPGEFIPFGLGSRYCPGNE 444

Query: 421 LAKLEMTVLLHHFLLGYR 437
           LA+LE+ +LLHHFLLGYR
Sbjct: 445 LARLEICILLHHFLLGYR 462

BLAST of MS005443 vs. NCBI nr
Match: XP_022157134.1 (beta-amyrin 11-oxidase-like isoform X1 [Momordica charantia])

HSP 1 Score: 709.9 bits (1831), Expect = 1.3e-200
Identity = 336/438 (76.71%), Postives = 388/438 (88.58%), Query Frame = 0

Query: 1   MRKFNDLWYRFRLGKNVYNALPPGDMGWPLVGSSLSFYQSFKIGGDPNSVMDNLISRFGR 60
           +R FN+LWY  RLG  +YNALPPG MGWPL+GSSLSFY++FKIGGDPNS++++L+SR+GR
Sbjct: 25  LRNFNELWYFLRLGGKIYNALPPGHMGWPLLGSSLSFYKAFKIGGDPNSIINHLVSRYGR 84

Query: 61  VRMYKSHLYGFPVIIVTDPEVCRRIYLDDEHFKLNYPKSVKLLQADGSLLKVDYKIVHRL 120
           VRMYKSHLYG PVIIVTDPEVCRR+YLDDE FKL+YPKSV +LQ D SLLK+DYKIVHRL
Sbjct: 85  VRMYKSHLYGSPVIIVTDPEVCRRVYLDDERFKLSYPKSVNILQGDRSLLKIDYKIVHRL 144

Query: 121 MAAPINGVEALSRYVEFIEDAVVEGLEEWGSMKGPVPLLHEFKRLTFKIIIGVFLGPVFT 180
           MAAPING EALS+Y+ FIE+AVV+GLEEWGS+KGPV LL EFKRLTFK+I GVFLGP FT
Sbjct: 145 MAAPINGSEALSKYMGFIEEAVVKGLEEWGSVKGPVELLLEFKRLTFKVITGVFLGPAFT 204

Query: 181 DSEVAELESLYAEIGPAIMSIFPYNLPGFSYRRALKARIKVEDIVRPVIQKRKKALENNV 240
           DSE+A+LE+LYAEIG AI+SIFPY+LPGFSYRRALKAR KVE+IVRPVI++R++ LE N 
Sbjct: 205 DSEIAQLENLYAEIGLAILSIFPYDLPGFSYRRALKARSKVENIVRPVIERRRRILEENE 264

Query: 241 DLEETCQLDAVIKATDKNGEKIFNDNAIICMLLGLLYAGYHTSAHGAQWTMVHVSENPDV 300
           +LEETCQ+DAVIKATDKNGEKIFNDNAI CMLLGLLYAGYHTSAHGAQWTMVHVSENPDV
Sbjct: 265 ELEETCQMDAVIKATDKNGEKIFNDNAIFCMLLGLLYAGYHTSAHGAQWTMVHVSENPDV 324

Query: 301 YQKAKEEQEMIIKQRPPTQKGLNFKEIKQMKYTAKI--ELIIYSLIIIFFNYCFLSSLLI 360
           YQKAKEEQEMIIKQRP TQKGLNFKEIKQMKY  K+  E++  + I+        + + +
Sbjct: 325 YQKAKEEQEMIIKQRPSTQKGLNFKEIKQMKYATKVINEMLRLNNIVFTTFRVATTDVNV 384

Query: 361 IGYTIPKGWIAAMWIRGVHMDDKIYENPLEFNPSRWDNYTPKPGEYIPFGIGSRFCPGNE 420
            GY IPKGW   +W RGVHMD +IY+NPLEF+PSRWDNY PKPGE+IPFG+GSR+CPGNE
Sbjct: 385 NGYIIPKGWAVPIWNRGVHMDPEIYQNPLEFDPSRWDNYVPKPGEFIPFGLGSRYCPGNE 444

Query: 421 LAKLEMTVLLHHFLLGYR 437
           LA+LE+ +LLHHFLLGYR
Sbjct: 445 LARLEICILLHHFLLGYR 462

BLAST of MS005443 vs. NCBI nr
Match: XP_022157141.1 (beta-amyrin 11-oxidase-like isoform X2 [Momordica charantia])

HSP 1 Score: 671.8 bits (1732), Expect = 4.0e-189
Identity = 320/413 (77.48%), Postives = 369/413 (89.35%), Query Frame = 0

Query: 26  MGWPLVGSSLSFYQSFKIGGDPNSVMDNLISRFGRVRMYKSHLYGFPVIIVTDPEVCRRI 85
           MGWPL+GSSLSFY++FKIGGDPNS++++L+SR+GRVRMYKSHLYG PVIIVTDPEVCRR+
Sbjct: 1   MGWPLLGSSLSFYKAFKIGGDPNSIINHLVSRYGRVRMYKSHLYGSPVIIVTDPEVCRRV 60

Query: 86  YLDDEHFKLNYPKSVKLLQADGSLLKVDYKIVHRLMAAPINGVEALSRYVEFIEDAVVEG 145
           YLDDE FKL+YPKSV +LQ D SLLK+DYKIVHRLMAAPING EALS+Y+ FIE+AVV+G
Sbjct: 61  YLDDERFKLSYPKSVNILQGDRSLLKIDYKIVHRLMAAPINGSEALSKYMGFIEEAVVKG 120

Query: 146 LEEWGSMKGPVPLLHEFKRLTFKIIIGVFLGPVFTDSEVAELESLYAEIGPAIMSIFPYN 205
           LEEWGS+KGPV LL EFKRLTFK+I GVFLGP FTDSE+A+LE+LYAEIG AI+SIFPY+
Sbjct: 121 LEEWGSVKGPVELLLEFKRLTFKVITGVFLGPAFTDSEIAQLENLYAEIGLAILSIFPYD 180

Query: 206 LPGFSYRRALKARIKVEDIVRPVIQKRKKALENNVDLEETCQLDAVIKATDKNGEKIFND 265
           LPGFSYRRALKAR KVE+IVRPVI++R++ LE N +LEETCQ+DAVIKATDKNGEKIFND
Sbjct: 181 LPGFSYRRALKARSKVENIVRPVIERRRRILEENEELEETCQMDAVIKATDKNGEKIFND 240

Query: 266 NAIICMLLGLLYAGYHTSAHGAQWTMVHVSENPDVYQKAKEEQEMIIKQRPPTQKGLNFK 325
           NAI CMLLGLLYAGYHTSAHGAQWTMVHVSENPDVYQKAKEEQEMIIKQRP TQKGLNFK
Sbjct: 241 NAIFCMLLGLLYAGYHTSAHGAQWTMVHVSENPDVYQKAKEEQEMIIKQRPSTQKGLNFK 300

Query: 326 EIKQMKYTAKI--ELIIYSLIIIFFNYCFLSSLLIIGYTIPKGWIAAMWIRGVHMDDKIY 385
           EIKQMKY  K+  E++  + I+        + + + GY IPKGW   +W RGVHMD +IY
Sbjct: 301 EIKQMKYATKVINEMLRLNNIVFTTFRVATTDVNVNGYIIPKGWAVPIWNRGVHMDPEIY 360

Query: 386 ENPLEFNPSRWDNYTPKPGEYIPFGIGSRFCPGNELAKLEMTVLLHHFLLGYR 437
           +NPLEF+PSRWDNY PKPGE+IPFG+GSR+CPGNELA+LE+ +LLHHFLLGYR
Sbjct: 361 QNPLEFDPSRWDNYVPKPGEFIPFGLGSRYCPGNELARLEICILLHHFLLGYR 413

BLAST of MS005443 vs. NCBI nr
Match: XP_022952147.1 (beta-amyrin 11-oxidase-like [Cucurbita moschata])

HSP 1 Score: 529.3 bits (1362), Expect = 3.2e-146
Identity = 247/438 (56.39%), Postives = 329/438 (75.11%), Query Frame = 0

Query: 1   MRKFNDLWYRFRLGKNVYNALPPGDMGWPLVGSSLSFYQSFKIGGDPNSVMDNLISRFGR 60
           +RK N++WY  +LG+  Y +LPPGDMGWPL+GSS SFY +F +GGDP+S + +L+SR GR
Sbjct: 20  VRKLNEIWYLVKLGRKAYKSLPPGDMGWPLLGSSFSFYNAFTVGGDPDSFIYHLLSRNGR 79

Query: 61  VRMYKSHLYGFPVIIVTDPEVCRRIYLDDEHFKLNYPKSVKLLQADGSLLKVDYKIVHRL 120
           V MYKSHLYG P IIVTDPE+CRRIYLDDE FK NYPKSVK+L+ +G   + D+K  +R+
Sbjct: 80  VGMYKSHLYGRPTIIVTDPEICRRIYLDDEIFKPNYPKSVKILEVNGFFTRADHKSAYRI 139

Query: 121 MAAPINGVEALSRYVEFIEDAVVEGLEEWGSMKGPVPLLHEFKRLTFKIIIGVFLGPVFT 180
           MAAP+NG E LS YV+FI+  + +GLEEW SMK P+ LL E   L FK+I  +FLG    
Sbjct: 140 MAAPMNGYEVLSHYVDFIDQVMAKGLEEWSSMKEPIELLGEIGSLFFKVISRIFLGTDIG 199

Query: 181 DSEVAELESLYAEIGPAIMSIFPYNLPGFSYRRALKARIKVEDIVRPVIQKRKKALENNV 240
            S +AELE+LY ++GPAI+SI PY++PG  Y RALKAR K+E+++  VI+++++ALE   
Sbjct: 200 ASRMAELETLYKDLGPAILSILPYDVPGLVYHRALKARKKIENVLECVIEEKRRALEQKE 259

Query: 241 DLEETCQLDAVIKATDKNGEKIFNDNAIICMLLGLLYAGYHTSAHGAQWTMVHVSENPDV 300
             E   Q+D +I A D+NG K++++N II +LLGLL+AG+HT A+ A W +VH+SE P +
Sbjct: 260 TREVHSQMDKLIMARDENGMKLYDNNTIIDLLLGLLHAGHHTPAYAAMWALVHISEKPHI 319

Query: 301 YQKAKEEQEMIIKQRPPTQKGLNFKEIKQMKYTAKI--ELIIYSLIIIFFNYCFLSSLLI 360
           + KAKEEQE I+KQRP TQKGL F+EIKQMKY  K   EL+  + I++       + + I
Sbjct: 320 FHKAKEEQESILKQRPSTQKGLTFQEIKQMKYLMKFINELLRRNTIVVASFRKAKTDINI 379

Query: 361 IGYTIPKGWIAAMWIRGVHMDDKIYENPLEFNPSRWDNYTPKPGEYIPFGIGSRFCPGNE 420
             Y IPKGW   +W R VHMD ++Y NP EF+PSRW+N++PKPG +IPFGIG+RFCPG+E
Sbjct: 380 NDYIIPKGWSVLIWTRAVHMDPQLYSNPEEFDPSRWENHSPKPGTFIPFGIGTRFCPGSE 439

Query: 421 LAKLEMTVLLHHFLLGYR 437
           L KLE+T+LLHHF+L Y+
Sbjct: 440 LTKLEITILLHHFILNYK 457

BLAST of MS005443 vs. ExPASy Swiss-Prot
Match: B5BSX1 (Beta-amyrin 11-oxidase OS=Glycyrrhiza uralensis OX=74613 GN=CYP88D6 PE=1 SV=1)

HSP 1 Score: 354.8 bits (909), Expect = 1.4e-96
Identity = 186/443 (41.99%), Postives = 288/443 (65.01%), Query Frame = 0

Query: 1   MRKFNDLWYRFRLGKNVYNALPPGDMGWPLVGSSLSFYQSFKIGGDPNSVMDNLISRFGR 60
           +R  N  +Y  +L +  +  LPPGDMGWPL+G  LSF + F   G P+S ++NL+ ++GR
Sbjct: 24  VRNLNGWYYDVKLRRKEH-PLPPGDMGWPLIGDLLSFIKDFS-SGHPDSFINNLVLKYGR 83

Query: 61  VRMYKSHLYGFPVIIVTDPEVCRRIYLDDEHFKLNYPKSVKLLQADGSLLKV---DYKIV 120
             +YK+HL+G P IIV +P++CRR+  DD +FKL YPKS+K L     ++ V   ++++ 
Sbjct: 84  SGIYKTHLFGNPSIIVCEPQMCRRVLTDDVNFKLGYPKSIKELARCRPMIDVSNAEHRLF 143

Query: 121 HRLMAAPINGVEALSRYVEFIEDAVVEGLEEWGSMKGPVPLLHEFKRLTFKIIIGVFLGP 180
            RL+ +PI G +AL+ Y+E +E+ V+  LEE  SMK PV LL E K+++FK I+ VF+G 
Sbjct: 144 RRLITSPIVGHKALAMYLERLEEIVINSLEELSSMKHPVELLKEMKKVSFKAIVHVFMGS 203

Query: 181 VFTDSEVAELESLYAEIGPAIMSIFPYNLPGFSYRRALKARIKVEDIVRPVIQKRKKALE 240
              D  + ++ S + ++   + SI P N+PGF++ +AL+AR K+  IV+PV+ +R+  +E
Sbjct: 204 SNQDI-IKKIGSSFTDLYNGMFSI-PINVPGFTFHKALEARKKLAKIVQPVVDERRLMIE 263

Query: 241 NNVD--LEETCQLDAVIKATDKNGEKIFNDNAIICMLLGLLYAGYHTSAHGAQWTMVHVS 300
           N      +    +D +++  D+NG K+  D  I  +L+GLL+AG+ ++A    W++ +++
Sbjct: 264 NGPQEGSQRKDLIDILLEVKDENGRKL-EDEDISDLLIGLLFAGHESTATSLMWSITYLT 323

Query: 301 ENPDVYQKAKEEQEMIIKQRPPTQKGLNFKEIKQMKYTAK-IELIIYSLIIIFFNYC-FL 360
           ++P + +KAKEEQE I + R  +QK L+ KEIKQM Y ++ I+  +    I F  +    
Sbjct: 324 QHPHILKKAKEEQEEITRTRFSSQKQLSLKEIKQMVYLSQVIDETLRCANIAFATFREAT 383

Query: 361 SSLLIIGYTIPKGWIAAMWIRGVHMDDKIYENPLEFNPSRWDNYTPKPGEYIPFGIGSRF 420
           + + I GY IPKGW   +W R +HMD + Y NP EFNPSRWD+Y  K G ++PFG GSR 
Sbjct: 384 ADVNINGYIIPKGWRVLIWARAIHMDSEYYPNPEEFNPSRWDDYNAKAGTFLPFGAGSRL 443

Query: 421 CPGNELAKLEMTVLLHHFLLGYR 437
           CPG +LAKLE+++ LH+FL  YR
Sbjct: 444 CPGADLAKLEISIFLHYFLRNYR 461

BLAST of MS005443 vs. ExPASy Swiss-Prot
Match: Q9C5Y2 (Ent-kaurenoic acid oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=KAO2 PE=1 SV=2)

HSP 1 Score: 345.9 bits (886), Expect = 6.7e-94
Identity = 181/443 (40.86%), Postives = 277/443 (62.53%), Query Frame = 0

Query: 1   MRKFNDLWYRFRLGKNVYNALPPGDMGWPLVGSSLSFYQSFKIGGDPNSVMDNLISRFGR 60
           +++ N   Y  +LG+  +  LPPGD+GWP++G+  SF ++FK   DP S + + I+R+GR
Sbjct: 25  LKRVNVWIYVSKLGEKKH-YLPPGDLGWPVIGNMWSFLRAFKT-SDPESFIQSYITRYGR 84

Query: 61  VRMYKSHLYGFPVIIVTDPEVCRRIYLDDEHFKLNYPKSVKLLQADGSLLKV---DYKIV 120
             +YK+H++G+P ++VT PE CRR+  DD+ F + +PKS   L    S + +   ++K +
Sbjct: 85  TGIYKAHMFGYPCVLVTTPETCRRVLTDDDAFHIGWPKSTMKLIGRKSFVGISFEEHKRL 144

Query: 121 HRLMAAPINGVEALSRYVEFIEDAVVEGLEEWGSMKGPVPLLHEFKRLTFKIIIGVFLGP 180
            RL +AP+NG EALS Y++FIE+ V   LE+W  M G +  L   ++LTFK+I+ +FL  
Sbjct: 145 RRLTSAPVNGPEALSVYIQFIEETVNTDLEKWSKM-GEIEFLSHLRKLTFKVIMYIFLSS 204

Query: 181 VFTDSEVAELESLYAEIGPAIMSIFPYNLPGFSYRRALKARIKVEDIVRPVIQKRKKALE 240
             ++  +  LE  Y  +   + ++   NLPGF+Y RALKAR K+    + ++  R+   +
Sbjct: 205 E-SEHVMDSLEREYTNLNYGVRAM-GINLPGFAYHRALKARKKLVAAFQSIVTNRRNQRK 264

Query: 241 NNVDLEETCQLDAVIKATDKNGEKIFNDNAIICMLLGLLYAGYHTSAHGAQWTMVHVSEN 300
            N+       LD +I   D+NG ++ +D  II +LL  L AG+ +S H   W  + + E+
Sbjct: 265 QNISSNRKDMLDNLIDVKDENG-RVLDDEEIIDLLLMYLNAGHESSGHLTMWATILMQEH 324

Query: 301 PDVYQKAKEEQEMIIKQRPPTQKGLNFKEIKQMKYTAKIELIIYSLIIIFFNYCFL---- 360
           P + QKAKEEQE I+K+R P QK L  KE ++M Y +++  I  +L +I F+        
Sbjct: 325 PMILQKAKEEQERIVKKRAPGQK-LTLKETREMVYLSQV--IDETLRVITFSLTAFREAK 384

Query: 361 SSLLIIGYTIPKGWIAAMWIRGVHMDDKIYENPLEFNPSRWDNYTPKPGEYIPFGIGSRF 420
           S + + GY IPKGW    W R VH+D +IY +P +F+PSRW+ YTPK G ++PFG+GS  
Sbjct: 385 SDVQMDGYIIPKGWKVLTWFRNVHLDPEIYPDPKKFDPSRWEGYTPKAGTFLPFGLGSHL 444

Query: 421 CPGNELAKLEMTVLLHHFLLGYR 437
           CPGN+LAKLE+++ LHHFLL YR
Sbjct: 445 CPGNDLAKLEISIFLHHFLLKYR 458

BLAST of MS005443 vs. ExPASy Swiss-Prot
Match: Q5VRM7 (Ent-kaurenoic acid oxidase OS=Oryza sativa subsp. japonica OX=39947 GN=KAO PE=2 SV=1)

HSP 1 Score: 329.7 bits (844), Expect = 4.9e-89
Identity = 172/445 (38.65%), Postives = 269/445 (60.45%), Query Frame = 0

Query: 1   MRKFNDLWYRF-RLGKNVYNALPPGDMGWPLVGSSLSFYQSFKIGGDPNSVMDNLISRFG 60
           +R+ +D W R   LG    + LPPG+MGWP+VGS  +F ++FK  G+P++ + + I RFG
Sbjct: 35  VRRAHD-WVRVAALGAERRSRLPPGEMGWPMVGSMWAFLRAFK-SGNPDAFIASFIRRFG 94

Query: 61  RVRMYKSHLYGFPVIIVTDPEVCRRIYLDDEHFKLNYPKSVKLLQADGSLLKV---DYKI 120
           R  +Y++ ++  P I+   PE C+++ +DDE F   +PK+   L    S + +   D++ 
Sbjct: 95  RTGVYRTFMFSSPTILAVTPEACKQVLMDDEGFVTGWPKATVTLIGPKSFVNMSYDDHRR 154

Query: 121 VHRLMAAPINGVEALSRYVEFIEDAVVEGLEEWGSMK-GPVPLLHEFKRLTFKIIIGVFL 180
           + +L AAPING +AL+ Y+ FI+  VV  L  W S + G V  L E +R+TFKII+ +F+
Sbjct: 155 IRKLTAAPINGFDALTTYLSFIDQTVVASLRRWSSPESGQVEFLTELRRMTFKIIVQIFM 214

Query: 181 GPVFTDSEVAELESLYAEIGPAIMSIFPYNLPGFSYRRALKARIKVEDIVRPVIQKRKKA 240
                D+ +  LE  Y ++    M     NLPGF+Y RAL+AR K+  +++ V+  R+ A
Sbjct: 215 SGA-DDATMEALERSYTDLNYG-MRAMAINLPGFAYYRALRARRKLVSVLQGVLDGRRAA 274

Query: 241 LENNVDLEETC-QLDAVIKATDKNGEKIFNDNAIICMLLGLLYAGYHTSAHGAQWTMVHV 300
                        +D +I+A D+ G ++ +D  I+ +L+  L AG+ +S H   W  V +
Sbjct: 275 AAKGFKRSGAMDMMDRLIEAEDERGRRLADDE-IVDVLIMYLNAGHESSGHITMWATVFL 334

Query: 301 SENPDVYQKAKEEQEMIIKQRPPTQKGLNFKEIKQMKYTAKI---ELIIYSLIIIFFNYC 360
            ENPD++ +AK EQE I++  P TQ GL  ++ K+M + +++    L   ++  + F   
Sbjct: 335 QENPDIFARAKAEQEEIMRSIPATQNGLTLRDFKKMHFLSQVVDETLRCVNISFVSFRQA 394

Query: 361 FLSSLLIIGYTIPKGWIAAMWIRGVHMDDKIYENPLEFNPSRWDNYTPKPGEYIPFGIGS 420
               + + GY IPKGW   +W R VHMDD++Y +P  FNPSRW+   PK G ++PFG+G+
Sbjct: 395 -TRDIFVNGYLIPKGWKVQLWYRSVHMDDQVYPDPKMFNPSRWEGPPPKAGTFLPFGLGA 454

Query: 421 RFCPGNELAKLEMTVLLHHFLLGYR 437
           R CPGN+LAKLE++V LHHFLLGY+
Sbjct: 455 RLCPGNDLAKLEISVFLHHFLLGYK 473

BLAST of MS005443 vs. ExPASy Swiss-Prot
Match: Q43246 (Cytochrome P450 88A1 OS=Zea mays OX=4577 GN=CYP88A1 PE=2 SV=1)

HSP 1 Score: 325.9 bits (834), Expect = 7.1e-88
Identity = 166/436 (38.07%), Postives = 264/436 (60.55%), Query Frame = 0

Query: 8   WYR-FRLGKNVYNALPPGDMGWPLVGSSLSFYQSFKIGGDPNSVMDNLISRFGRVRMYKS 67
           WYR   LG      LPPG+MGWPLVG   +F ++FK  G P++ + + + RFGR  +Y+S
Sbjct: 58  WYREAALGAARRARLPPGEMGWPLVGGMWAFLRAFK-SGKPDAFIASFVRRFGRTGVYRS 117

Query: 68  HLYGFPVIIVTDPEVCRRIYLDDEHFKLNYPKSVKLLQADGSLLKVDY---KIVHRLMAA 127
            ++  P ++VT  E C+++ +DD+ F   +PK+   L    S + + Y   + + +L AA
Sbjct: 118 FMFSSPTVLVTTAEGCKQVLMDDDAFVTGWPKATVALVGPRSFVAMPYDEHRRIRKLTAA 177

Query: 128 PINGVEALSRYVEFIEDAVVEGLEEWGSMKGPVPLLHEFKRLTFKIIIGVFLGPVFTDSE 187
           PING +AL+ Y+ FI+  V   L  W    G V  L E +R+TFKII+ +FLG     + 
Sbjct: 178 PINGFDALTGYLPFIDRTVTSSLRAWADHGGSVEFLTELRRMTFKIIVQIFLGGA-DQAT 237

Query: 188 VAELESLYAEIGPAIMSIFPYNLPGFSYRRALKARIKVEDIVRPVIQKRKKALENNVDLE 247
              LE  Y E+    M     NLPGF+YR AL+AR ++  +++ V+ +R+ A    V   
Sbjct: 238 TRALERSYTELNYG-MRAMAINLPGFAYRGALRARRRLVAVLQGVLDERRAARAKGVSGG 297

Query: 248 ETCQLDAVIKATDKNGEKIFNDNAIICMLLGLLYAGYHTSAHGAQWTMVHVSENPDVYQK 307
               +D +I+A D+ G  + +D+ II +L+  L AG+ +S H   W  V + ENPD++ +
Sbjct: 298 GVDMMDRLIEAQDERGRHL-DDDEIIDVLVMYLNAGHESSGHITMWATVFLQENPDMFAR 357

Query: 308 AKEEQEMIIKQRPPTQKGLNFKEIKQMKYTAKI---ELIIYSLIIIFFNYCFLSSLLIIG 367
           AK EQE I++  P +Q+GL  ++ ++M+Y +++    L + ++  + F       + + G
Sbjct: 358 AKAEQEAIMRSIPSSQRGLTLRDFRKMEYLSQVIDETLRLVNISFVSFRQA-TRDVFVNG 417

Query: 368 YTIPKGWIAAMWIRGVHMDDKIYENPLEFNPSRWDNYTPKPGEYIPFGIGSRFCPGNELA 427
           Y IPKGW   +W R VHMD ++Y +P +F+PSRW+ ++P+ G ++ FG+G+R CPGN+LA
Sbjct: 418 YLIPKGWKVQLWYRSVHMDPQVYPDPTKFDPSRWEGHSPRAGTFLAFGLGARLCPGNDLA 477

Query: 428 KLEMTVLLHHFLLGYR 437
           KLE++V LHHFLLGY+
Sbjct: 478 KLEISVFLHHFLLGYK 488

BLAST of MS005443 vs. ExPASy Swiss-Prot
Match: O23051 (Ent-kaurenoic acid oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=KAO1 PE=1 SV=1)

HSP 1 Score: 325.1 bits (832), Expect = 1.2e-87
Identity = 176/444 (39.64%), Postives = 274/444 (61.71%), Query Frame = 0

Query: 1   MRKFNDLWYRFRLGKNVYNALPPGDMGWPLVGSSLSFYQSFKIGGDPNSVMDNLISRFGR 60
           +RK N   Y   LG+N  + LPPGD+GWP +G+ LSF ++FK   DP+S    LI R+G 
Sbjct: 26  VRKVNVWLYESSLGEN-RHYLPPGDLGWPFIGNMLSFLRAFKT-SDPDSFTRTLIKRYGP 85

Query: 61  VRMYKSHLYGFPVIIVTDPEVCRRIYLDDEHFKLNYPKSVKLLQADGSLLKV---DYKIV 120
             +YK+H++G P IIVT  + CRR+  DD+ FK  +P S   L    S + +   ++K +
Sbjct: 86  KGIYKAHMFGNPSIIVTTSDTCRRVLTDDDAFKPGWPTSTMELIGRKSFVGISFEEHKRL 145

Query: 121 HRLMAAPINGVEALSRYVEFIEDAVVEGLEEWGSMKGPVPLLHEFKRLTFKIIIGVFLGP 180
            RL AAP+NG EALS Y+ +IE+ V+  L++W  M G    L   ++LTF+II+ +FL  
Sbjct: 146 RRLTAAPVNGHEALSTYIPYIEENVITVLDKWTKM-GEFEFLTHLRKLTFRIIMYIFLSS 205

Query: 181 VFTDSEVAELESLYAEIGPAIMSIFPYNLPGFSYRRALKARIKVEDIVRPVIQKRKKALE 240
             +++ +  LE  Y  +   + ++   N+PGF+Y RALKAR  +    + ++ +R+   +
Sbjct: 206 E-SENVMDALEREYTALNYGVRAM-AVNIPGFAYHRALKARKTLVAAFQSIVTERRNQRK 265

Query: 241 NNVDLEETCQLDAVIKATDKNGEKIFNDNAIICMLLGLLYAGYHTSAHGAQWTMVHVSEN 300
            N+   +   LD ++   D++G K  +D  II +LL  L AG+ +S H   W  V + E+
Sbjct: 266 QNILSNKKDMLDNLLNVKDEDG-KTLDDEEIIDVLLMYLNAGHESSGHTIMWATVFLQEH 325

Query: 301 PDVYQKAKEEQEMIIKQRPPTQKGLNFKEIKQMKYTAKIELIIYSLIIIFFNYCFL---- 360
           P+V Q+AK EQEMI+K RP  QKGL+ KE ++M++ +++  +  +L +I F+        
Sbjct: 326 PEVLQRAKAEQEMILKSRPEGQKGLSLKETRKMEFLSQV--VDETLRVITFSLTAFREAK 385

Query: 361 SSLLIIGYTIPKGWIAAMWIRGVHMDDKIYENPLEFNPSRWDN-YTPKPGEYIPFGIGSR 420
           + + + GY IPKGW    W R VH+D +++ +P +F+P+RWDN + PK G ++PFG GS 
Sbjct: 386 TDVEMNGYLIPKGWKVLTWFRDVHIDPEVFPDPRKFDPARWDNGFVPKAGAFLPFGAGSH 445

Query: 421 FCPGNELAKLEMTVLLHHFLLGYR 437
            CPGN+LAKLE+++ LHHFLL Y+
Sbjct: 446 LCPGNDLAKLEISIFLHHFLLKYQ 461

BLAST of MS005443 vs. ExPASy TrEMBL
Match: A0A5K7U6A4 (Cytochrome P450 monooxygenase OS=Momordica charantia OX=3673 GN=McCYP88L7 PE=2 SV=1)

HSP 1 Score: 832.0 bits (2148), Expect = 1.1e-237
Identity = 407/438 (92.92%), Postives = 421/438 (96.12%), Query Frame = 0

Query: 1   MRKFNDLWYRFRLGKNVYNALPPGDMGWPLVGSSLSFYQSFKIGGDPNSVMDNLISRFGR 60
           +RKFND+WYRF+LGKNVYNALPPGDMGWPLVGSSLSFYQ+FKIGGDPN+VMDNLISRFGR
Sbjct: 25  LRKFNDIWYRFKLGKNVYNALPPGDMGWPLVGSSLSFYQAFKIGGDPNAVMDNLISRFGR 84

Query: 61  VRMYKSHLYGFPVIIVTDPEVCRRIYLDDEHFKLNYPKSVKLLQADGSLLKVDYKIVHRL 120
           VRMYKSHLYGFPVIIVTDPEVCRRIYLDDEHFKLNYPKSVKLLQADGSLLKVDYKIVHRL
Sbjct: 85  VRMYKSHLYGFPVIIVTDPEVCRRIYLDDEHFKLNYPKSVKLLQADGSLLKVDYKIVHRL 144

Query: 121 MAAPINGVEALSRYVEFIEDAVVEGLEEWGSMKGPVPLLHEFKRLTFKIIIGVFLGPVFT 180
           MAAPINGVEALSRYVEFIEDAVVEGLEEWGSMKGPVPLLHEFKRLTFKIIIGVFLGPVFT
Sbjct: 145 MAAPINGVEALSRYVEFIEDAVVEGLEEWGSMKGPVPLLHEFKRLTFKIIIGVFLGPVFT 204

Query: 181 DSEVAELESLYAEIGPAIMSIFPYNLPGFSYRRALKARIKVEDIVRPVIQKRKKALENNV 240
           DSEVAELESLYAEIGPAIMSIFPYNLPGFSYRRALKARIKVE+IVRPVIQ RKKALENNV
Sbjct: 205 DSEVAELESLYAEIGPAIMSIFPYNLPGFSYRRALKARIKVENIVRPVIQNRKKALENNV 264

Query: 241 DLEETCQLDAVIKATDKNGEKIFNDNAIICMLLGLLYAGYHTSAHGAQWTMVHVSENPDV 300
           DLEETCQLDAVIKATDKNGE++FNDNAIICMLLGLLYAGYHTSAHGAQWTMVHVSENPDV
Sbjct: 265 DLEETCQLDAVIKATDKNGERVFNDNAIICMLLGLLYAGYHTSAHGAQWTMVHVSENPDV 324

Query: 301 YQKAKEEQEMIIKQRPPTQKGLNFKEIKQMKYTAKIELIIYSL-IIIFFNYCFLS-SLLI 360
           YQKAKEEQEMIIKQRPP QKGLNFKEIKQMKYTAKI   +  L  I+F N+   +  + +
Sbjct: 325 YQKAKEEQEMIIKQRPPNQKGLNFKEIKQMKYTAKIINEVLRLNNIVFTNFRMATKDVNV 384

Query: 361 IGYTIPKGWIAAMWIRGVHMDDKIYENPLEFNPSRWDNYTPKPGEYIPFGIGSRFCPGNE 420
            GYTIPKGWIAAMWIRGVHMDDKIYENPLEFNPSRWDNYTPKPGEYIPFGIGSRFCPGNE
Sbjct: 385 NGYTIPKGWIAAMWIRGVHMDDKIYENPLEFNPSRWDNYTPKPGEYIPFGIGSRFCPGNE 444

Query: 421 LAKLEMTVLLHHFLLGYR 437
           LAKLEMTVLLHHFLLGYR
Sbjct: 445 LAKLEMTVLLHHFLLGYR 462

BLAST of MS005443 vs. ExPASy TrEMBL
Match: A0A5K7U6M8 (Cytochrome P450 monooxygenase OS=Momordica charantia OX=3673 GN=McCYP88L8 PE=2 SV=1)

HSP 1 Score: 711.4 bits (1835), Expect = 2.2e-201
Identity = 337/438 (76.94%), Postives = 389/438 (88.81%), Query Frame = 0

Query: 1   MRKFNDLWYRFRLGKNVYNALPPGDMGWPLVGSSLSFYQSFKIGGDPNSVMDNLISRFGR 60
           +R FN+LWY  RLG  +YNALPPG MGWPL+GSSLSFY++FKIGGDPNS++++L+SR+GR
Sbjct: 25  LRNFNELWYFLRLGGKIYNALPPGHMGWPLLGSSLSFYKAFKIGGDPNSIINHLVSRYGR 84

Query: 61  VRMYKSHLYGFPVIIVTDPEVCRRIYLDDEHFKLNYPKSVKLLQADGSLLKVDYKIVHRL 120
           VRMYKSHLYG PVIIVTDPEVCRR+YLDDE FKL+YPKSV +LQ D SLLK+DYKIVHRL
Sbjct: 85  VRMYKSHLYGSPVIIVTDPEVCRRVYLDDERFKLSYPKSVNILQGDRSLLKIDYKIVHRL 144

Query: 121 MAAPINGVEALSRYVEFIEDAVVEGLEEWGSMKGPVPLLHEFKRLTFKIIIGVFLGPVFT 180
           MAAPING EALS+Y+ FIE+AVV+GLEEWGS+KGPV LL EFKRLTFK+I GVFLGP FT
Sbjct: 145 MAAPINGSEALSKYMGFIEEAVVKGLEEWGSVKGPVELLLEFKRLTFKVITGVFLGPAFT 204

Query: 181 DSEVAELESLYAEIGPAIMSIFPYNLPGFSYRRALKARIKVEDIVRPVIQKRKKALENNV 240
           DSE+A+LE+LYAEIG AI+SIFPY+LPGFSYRRALKAR KVE+IVRPVI++R++ LE N 
Sbjct: 205 DSEIAQLENLYAEIGLAILSIFPYDLPGFSYRRALKARSKVENIVRPVIERRRRILEENE 264

Query: 241 DLEETCQLDAVIKATDKNGEKIFNDNAIICMLLGLLYAGYHTSAHGAQWTMVHVSENPDV 300
           +LEETCQ+DAVIKATDKNGEKIFNDNAIICMLLGLLYAGYHTSAHGAQWTMVHVSENPDV
Sbjct: 265 ELEETCQMDAVIKATDKNGEKIFNDNAIICMLLGLLYAGYHTSAHGAQWTMVHVSENPDV 324

Query: 301 YQKAKEEQEMIIKQRPPTQKGLNFKEIKQMKYTAKI--ELIIYSLIIIFFNYCFLSSLLI 360
           YQKAKEEQEMIIKQRP TQKGLNFKEIKQMKY  K+  E++  + I+        + + +
Sbjct: 325 YQKAKEEQEMIIKQRPSTQKGLNFKEIKQMKYATKVINEMLRLNNIVFTTFRVATTDVNV 384

Query: 361 IGYTIPKGWIAAMWIRGVHMDDKIYENPLEFNPSRWDNYTPKPGEYIPFGIGSRFCPGNE 420
            GY IPKGW   +W RGVHMD +IY+NPLEF+PSRWDNY PKPGE+IPFG+GSR+CPGNE
Sbjct: 385 NGYIIPKGWAVPIWNRGVHMDPEIYQNPLEFDPSRWDNYVPKPGEFIPFGLGSRYCPGNE 444

Query: 421 LAKLEMTVLLHHFLLGYR 437
           LA+LE+ +LLHHFLLGYR
Sbjct: 445 LARLEICILLHHFLLGYR 462

BLAST of MS005443 vs. ExPASy TrEMBL
Match: A0A6J1DVM6 (beta-amyrin 11-oxidase-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111023928 PE=3 SV=1)

HSP 1 Score: 709.9 bits (1831), Expect = 6.5e-201
Identity = 336/438 (76.71%), Postives = 388/438 (88.58%), Query Frame = 0

Query: 1   MRKFNDLWYRFRLGKNVYNALPPGDMGWPLVGSSLSFYQSFKIGGDPNSVMDNLISRFGR 60
           +R FN+LWY  RLG  +YNALPPG MGWPL+GSSLSFY++FKIGGDPNS++++L+SR+GR
Sbjct: 25  LRNFNELWYFLRLGGKIYNALPPGHMGWPLLGSSLSFYKAFKIGGDPNSIINHLVSRYGR 84

Query: 61  VRMYKSHLYGFPVIIVTDPEVCRRIYLDDEHFKLNYPKSVKLLQADGSLLKVDYKIVHRL 120
           VRMYKSHLYG PVIIVTDPEVCRR+YLDDE FKL+YPKSV +LQ D SLLK+DYKIVHRL
Sbjct: 85  VRMYKSHLYGSPVIIVTDPEVCRRVYLDDERFKLSYPKSVNILQGDRSLLKIDYKIVHRL 144

Query: 121 MAAPINGVEALSRYVEFIEDAVVEGLEEWGSMKGPVPLLHEFKRLTFKIIIGVFLGPVFT 180
           MAAPING EALS+Y+ FIE+AVV+GLEEWGS+KGPV LL EFKRLTFK+I GVFLGP FT
Sbjct: 145 MAAPINGSEALSKYMGFIEEAVVKGLEEWGSVKGPVELLLEFKRLTFKVITGVFLGPAFT 204

Query: 181 DSEVAELESLYAEIGPAIMSIFPYNLPGFSYRRALKARIKVEDIVRPVIQKRKKALENNV 240
           DSE+A+LE+LYAEIG AI+SIFPY+LPGFSYRRALKAR KVE+IVRPVI++R++ LE N 
Sbjct: 205 DSEIAQLENLYAEIGLAILSIFPYDLPGFSYRRALKARSKVENIVRPVIERRRRILEENE 264

Query: 241 DLEETCQLDAVIKATDKNGEKIFNDNAIICMLLGLLYAGYHTSAHGAQWTMVHVSENPDV 300
           +LEETCQ+DAVIKATDKNGEKIFNDNAI CMLLGLLYAGYHTSAHGAQWTMVHVSENPDV
Sbjct: 265 ELEETCQMDAVIKATDKNGEKIFNDNAIFCMLLGLLYAGYHTSAHGAQWTMVHVSENPDV 324

Query: 301 YQKAKEEQEMIIKQRPPTQKGLNFKEIKQMKYTAKI--ELIIYSLIIIFFNYCFLSSLLI 360
           YQKAKEEQEMIIKQRP TQKGLNFKEIKQMKY  K+  E++  + I+        + + +
Sbjct: 325 YQKAKEEQEMIIKQRPSTQKGLNFKEIKQMKYATKVINEMLRLNNIVFTTFRVATTDVNV 384

Query: 361 IGYTIPKGWIAAMWIRGVHMDDKIYENPLEFNPSRWDNYTPKPGEYIPFGIGSRFCPGNE 420
            GY IPKGW   +W RGVHMD +IY+NPLEF+PSRWDNY PKPGE+IPFG+GSR+CPGNE
Sbjct: 385 NGYIIPKGWAVPIWNRGVHMDPEIYQNPLEFDPSRWDNYVPKPGEFIPFGLGSRYCPGNE 444

Query: 421 LAKLEMTVLLHHFLLGYR 437
           LA+LE+ +LLHHFLLGYR
Sbjct: 445 LARLEICILLHHFLLGYR 462

BLAST of MS005443 vs. ExPASy TrEMBL
Match: A0A6J1DX36 (beta-amyrin 11-oxidase-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111023928 PE=3 SV=1)

HSP 1 Score: 671.8 bits (1732), Expect = 2.0e-189
Identity = 320/413 (77.48%), Postives = 369/413 (89.35%), Query Frame = 0

Query: 26  MGWPLVGSSLSFYQSFKIGGDPNSVMDNLISRFGRVRMYKSHLYGFPVIIVTDPEVCRRI 85
           MGWPL+GSSLSFY++FKIGGDPNS++++L+SR+GRVRMYKSHLYG PVIIVTDPEVCRR+
Sbjct: 1   MGWPLLGSSLSFYKAFKIGGDPNSIINHLVSRYGRVRMYKSHLYGSPVIIVTDPEVCRRV 60

Query: 86  YLDDEHFKLNYPKSVKLLQADGSLLKVDYKIVHRLMAAPINGVEALSRYVEFIEDAVVEG 145
           YLDDE FKL+YPKSV +LQ D SLLK+DYKIVHRLMAAPING EALS+Y+ FIE+AVV+G
Sbjct: 61  YLDDERFKLSYPKSVNILQGDRSLLKIDYKIVHRLMAAPINGSEALSKYMGFIEEAVVKG 120

Query: 146 LEEWGSMKGPVPLLHEFKRLTFKIIIGVFLGPVFTDSEVAELESLYAEIGPAIMSIFPYN 205
           LEEWGS+KGPV LL EFKRLTFK+I GVFLGP FTDSE+A+LE+LYAEIG AI+SIFPY+
Sbjct: 121 LEEWGSVKGPVELLLEFKRLTFKVITGVFLGPAFTDSEIAQLENLYAEIGLAILSIFPYD 180

Query: 206 LPGFSYRRALKARIKVEDIVRPVIQKRKKALENNVDLEETCQLDAVIKATDKNGEKIFND 265
           LPGFSYRRALKAR KVE+IVRPVI++R++ LE N +LEETCQ+DAVIKATDKNGEKIFND
Sbjct: 181 LPGFSYRRALKARSKVENIVRPVIERRRRILEENEELEETCQMDAVIKATDKNGEKIFND 240

Query: 266 NAIICMLLGLLYAGYHTSAHGAQWTMVHVSENPDVYQKAKEEQEMIIKQRPPTQKGLNFK 325
           NAI CMLLGLLYAGYHTSAHGAQWTMVHVSENPDVYQKAKEEQEMIIKQRP TQKGLNFK
Sbjct: 241 NAIFCMLLGLLYAGYHTSAHGAQWTMVHVSENPDVYQKAKEEQEMIIKQRPSTQKGLNFK 300

Query: 326 EIKQMKYTAKI--ELIIYSLIIIFFNYCFLSSLLIIGYTIPKGWIAAMWIRGVHMDDKIY 385
           EIKQMKY  K+  E++  + I+        + + + GY IPKGW   +W RGVHMD +IY
Sbjct: 301 EIKQMKYATKVINEMLRLNNIVFTTFRVATTDVNVNGYIIPKGWAVPIWNRGVHMDPEIY 360

Query: 386 ENPLEFNPSRWDNYTPKPGEYIPFGIGSRFCPGNELAKLEMTVLLHHFLLGYR 437
           +NPLEF+PSRWDNY PKPGE+IPFG+GSR+CPGNELA+LE+ +LLHHFLLGYR
Sbjct: 361 QNPLEFDPSRWDNYVPKPGEFIPFGLGSRYCPGNELARLEICILLHHFLLGYR 413

BLAST of MS005443 vs. ExPASy TrEMBL
Match: A0A6J1GJM1 (beta-amyrin 11-oxidase-like OS=Cucurbita moschata OX=3662 GN=LOC111454910 PE=3 SV=1)

HSP 1 Score: 529.3 bits (1362), Expect = 1.6e-146
Identity = 247/438 (56.39%), Postives = 329/438 (75.11%), Query Frame = 0

Query: 1   MRKFNDLWYRFRLGKNVYNALPPGDMGWPLVGSSLSFYQSFKIGGDPNSVMDNLISRFGR 60
           +RK N++WY  +LG+  Y +LPPGDMGWPL+GSS SFY +F +GGDP+S + +L+SR GR
Sbjct: 20  VRKLNEIWYLVKLGRKAYKSLPPGDMGWPLLGSSFSFYNAFTVGGDPDSFIYHLLSRNGR 79

Query: 61  VRMYKSHLYGFPVIIVTDPEVCRRIYLDDEHFKLNYPKSVKLLQADGSLLKVDYKIVHRL 120
           V MYKSHLYG P IIVTDPE+CRRIYLDDE FK NYPKSVK+L+ +G   + D+K  +R+
Sbjct: 80  VGMYKSHLYGRPTIIVTDPEICRRIYLDDEIFKPNYPKSVKILEVNGFFTRADHKSAYRI 139

Query: 121 MAAPINGVEALSRYVEFIEDAVVEGLEEWGSMKGPVPLLHEFKRLTFKIIIGVFLGPVFT 180
           MAAP+NG E LS YV+FI+  + +GLEEW SMK P+ LL E   L FK+I  +FLG    
Sbjct: 140 MAAPMNGYEVLSHYVDFIDQVMAKGLEEWSSMKEPIELLGEIGSLFFKVISRIFLGTDIG 199

Query: 181 DSEVAELESLYAEIGPAIMSIFPYNLPGFSYRRALKARIKVEDIVRPVIQKRKKALENNV 240
            S +AELE+LY ++GPAI+SI PY++PG  Y RALKAR K+E+++  VI+++++ALE   
Sbjct: 200 ASRMAELETLYKDLGPAILSILPYDVPGLVYHRALKARKKIENVLECVIEEKRRALEQKE 259

Query: 241 DLEETCQLDAVIKATDKNGEKIFNDNAIICMLLGLLYAGYHTSAHGAQWTMVHVSENPDV 300
             E   Q+D +I A D+NG K++++N II +LLGLL+AG+HT A+ A W +VH+SE P +
Sbjct: 260 TREVHSQMDKLIMARDENGMKLYDNNTIIDLLLGLLHAGHHTPAYAAMWALVHISEKPHI 319

Query: 301 YQKAKEEQEMIIKQRPPTQKGLNFKEIKQMKYTAKI--ELIIYSLIIIFFNYCFLSSLLI 360
           + KAKEEQE I+KQRP TQKGL F+EIKQMKY  K   EL+  + I++       + + I
Sbjct: 320 FHKAKEEQESILKQRPSTQKGLTFQEIKQMKYLMKFINELLRRNTIVVASFRKAKTDINI 379

Query: 361 IGYTIPKGWIAAMWIRGVHMDDKIYENPLEFNPSRWDNYTPKPGEYIPFGIGSRFCPGNE 420
             Y IPKGW   +W R VHMD ++Y NP EF+PSRW+N++PKPG +IPFGIG+RFCPG+E
Sbjct: 380 NDYIIPKGWSVLIWTRAVHMDPQLYSNPEEFDPSRWENHSPKPGTFIPFGIGTRFCPGSE 439

Query: 421 LAKLEMTVLLHHFLLGYR 437
           L KLE+T+LLHHF+L Y+
Sbjct: 440 LTKLEITILLHHFILNYK 457

BLAST of MS005443 vs. TAIR 10
Match: AT2G32440.1 (ent-kaurenoic acid hydroxylase 2 )

HSP 1 Score: 345.9 bits (886), Expect = 4.7e-95
Identity = 181/443 (40.86%), Postives = 277/443 (62.53%), Query Frame = 0

Query: 1   MRKFNDLWYRFRLGKNVYNALPPGDMGWPLVGSSLSFYQSFKIGGDPNSVMDNLISRFGR 60
           +++ N   Y  +LG+  +  LPPGD+GWP++G+  SF ++FK   DP S + + I+R+GR
Sbjct: 25  LKRVNVWIYVSKLGEKKH-YLPPGDLGWPVIGNMWSFLRAFKT-SDPESFIQSYITRYGR 84

Query: 61  VRMYKSHLYGFPVIIVTDPEVCRRIYLDDEHFKLNYPKSVKLLQADGSLLKV---DYKIV 120
             +YK+H++G+P ++VT PE CRR+  DD+ F + +PKS   L    S + +   ++K +
Sbjct: 85  TGIYKAHMFGYPCVLVTTPETCRRVLTDDDAFHIGWPKSTMKLIGRKSFVGISFEEHKRL 144

Query: 121 HRLMAAPINGVEALSRYVEFIEDAVVEGLEEWGSMKGPVPLLHEFKRLTFKIIIGVFLGP 180
            RL +AP+NG EALS Y++FIE+ V   LE+W  M G +  L   ++LTFK+I+ +FL  
Sbjct: 145 RRLTSAPVNGPEALSVYIQFIEETVNTDLEKWSKM-GEIEFLSHLRKLTFKVIMYIFLSS 204

Query: 181 VFTDSEVAELESLYAEIGPAIMSIFPYNLPGFSYRRALKARIKVEDIVRPVIQKRKKALE 240
             ++  +  LE  Y  +   + ++   NLPGF+Y RALKAR K+    + ++  R+   +
Sbjct: 205 E-SEHVMDSLEREYTNLNYGVRAM-GINLPGFAYHRALKARKKLVAAFQSIVTNRRNQRK 264

Query: 241 NNVDLEETCQLDAVIKATDKNGEKIFNDNAIICMLLGLLYAGYHTSAHGAQWTMVHVSEN 300
            N+       LD +I   D+NG ++ +D  II +LL  L AG+ +S H   W  + + E+
Sbjct: 265 QNISSNRKDMLDNLIDVKDENG-RVLDDEEIIDLLLMYLNAGHESSGHLTMWATILMQEH 324

Query: 301 PDVYQKAKEEQEMIIKQRPPTQKGLNFKEIKQMKYTAKIELIIYSLIIIFFNYCFL---- 360
           P + QKAKEEQE I+K+R P QK L  KE ++M Y +++  I  +L +I F+        
Sbjct: 325 PMILQKAKEEQERIVKKRAPGQK-LTLKETREMVYLSQV--IDETLRVITFSLTAFREAK 384

Query: 361 SSLLIIGYTIPKGWIAAMWIRGVHMDDKIYENPLEFNPSRWDNYTPKPGEYIPFGIGSRF 420
           S + + GY IPKGW    W R VH+D +IY +P +F+PSRW+ YTPK G ++PFG+GS  
Sbjct: 385 SDVQMDGYIIPKGWKVLTWFRNVHLDPEIYPDPKKFDPSRWEGYTPKAGTFLPFGLGSHL 444

Query: 421 CPGNELAKLEMTVLLHHFLLGYR 437
           CPGN+LAKLE+++ LHHFLL YR
Sbjct: 445 CPGNDLAKLEISIFLHHFLLKYR 458

BLAST of MS005443 vs. TAIR 10
Match: AT2G32440.2 (ent-kaurenoic acid hydroxylase 2 )

HSP 1 Score: 345.9 bits (886), Expect = 4.7e-95
Identity = 181/443 (40.86%), Postives = 277/443 (62.53%), Query Frame = 0

Query: 1   MRKFNDLWYRFRLGKNVYNALPPGDMGWPLVGSSLSFYQSFKIGGDPNSVMDNLISRFGR 60
           +++ N   Y  +LG+  +  LPPGD+GWP++G+  SF ++FK   DP S + + I+R+GR
Sbjct: 25  LKRVNVWIYVSKLGEKKH-YLPPGDLGWPVIGNMWSFLRAFKT-SDPESFIQSYITRYGR 84

Query: 61  VRMYKSHLYGFPVIIVTDPEVCRRIYLDDEHFKLNYPKSVKLLQADGSLLKV---DYKIV 120
             +YK+H++G+P ++VT PE CRR+  DD+ F + +PKS   L    S + +   ++K +
Sbjct: 85  TGIYKAHMFGYPCVLVTTPETCRRVLTDDDAFHIGWPKSTMKLIGRKSFVGISFEEHKRL 144

Query: 121 HRLMAAPINGVEALSRYVEFIEDAVVEGLEEWGSMKGPVPLLHEFKRLTFKIIIGVFLGP 180
            RL +AP+NG EALS Y++FIE+ V   LE+W  M G +  L   ++LTFK+I+ +FL  
Sbjct: 145 RRLTSAPVNGPEALSVYIQFIEETVNTDLEKWSKM-GEIEFLSHLRKLTFKVIMYIFLSS 204

Query: 181 VFTDSEVAELESLYAEIGPAIMSIFPYNLPGFSYRRALKARIKVEDIVRPVIQKRKKALE 240
             ++  +  LE  Y  +   + ++   NLPGF+Y RALKAR K+    + ++  R+   +
Sbjct: 205 E-SEHVMDSLEREYTNLNYGVRAM-GINLPGFAYHRALKARKKLVAAFQSIVTNRRNQRK 264

Query: 241 NNVDLEETCQLDAVIKATDKNGEKIFNDNAIICMLLGLLYAGYHTSAHGAQWTMVHVSEN 300
            N+       LD +I   D+NG ++ +D  II +LL  L AG+ +S H   W  + + E+
Sbjct: 265 QNISSNRKDMLDNLIDVKDENG-RVLDDEEIIDLLLMYLNAGHESSGHLTMWATILMQEH 324

Query: 301 PDVYQKAKEEQEMIIKQRPPTQKGLNFKEIKQMKYTAKIELIIYSLIIIFFNYCFL---- 360
           P + QKAKEEQE I+K+R P QK L  KE ++M Y +++  I  +L +I F+        
Sbjct: 325 PMILQKAKEEQERIVKKRAPGQK-LTLKETREMVYLSQV--IDETLRVITFSLTAFREAK 384

Query: 361 SSLLIIGYTIPKGWIAAMWIRGVHMDDKIYENPLEFNPSRWDNYTPKPGEYIPFGIGSRF 420
           S + + GY IPKGW    W R VH+D +IY +P +F+PSRW+ YTPK G ++PFG+GS  
Sbjct: 385 SDVQMDGYIIPKGWKVLTWFRNVHLDPEIYPDPKKFDPSRWEGYTPKAGTFLPFGLGSHL 444

Query: 421 CPGNELAKLEMTVLLHHFLLGYR 437
           CPGN+LAKLE+++ LHHFLL YR
Sbjct: 445 CPGNDLAKLEISIFLHHFLLKYR 458

BLAST of MS005443 vs. TAIR 10
Match: AT1G05160.1 (cytochrome P450, family 88, subfamily A, polypeptide 3 )

HSP 1 Score: 325.1 bits (832), Expect = 8.7e-89
Identity = 176/444 (39.64%), Postives = 274/444 (61.71%), Query Frame = 0

Query: 1   MRKFNDLWYRFRLGKNVYNALPPGDMGWPLVGSSLSFYQSFKIGGDPNSVMDNLISRFGR 60
           +RK N   Y   LG+N  + LPPGD+GWP +G+ LSF ++FK   DP+S    LI R+G 
Sbjct: 26  VRKVNVWLYESSLGEN-RHYLPPGDLGWPFIGNMLSFLRAFKT-SDPDSFTRTLIKRYGP 85

Query: 61  VRMYKSHLYGFPVIIVTDPEVCRRIYLDDEHFKLNYPKSVKLLQADGSLLKV---DYKIV 120
             +YK+H++G P IIVT  + CRR+  DD+ FK  +P S   L    S + +   ++K +
Sbjct: 86  KGIYKAHMFGNPSIIVTTSDTCRRVLTDDDAFKPGWPTSTMELIGRKSFVGISFEEHKRL 145

Query: 121 HRLMAAPINGVEALSRYVEFIEDAVVEGLEEWGSMKGPVPLLHEFKRLTFKIIIGVFLGP 180
            RL AAP+NG EALS Y+ +IE+ V+  L++W  M G    L   ++LTF+II+ +FL  
Sbjct: 146 RRLTAAPVNGHEALSTYIPYIEENVITVLDKWTKM-GEFEFLTHLRKLTFRIIMYIFLSS 205

Query: 181 VFTDSEVAELESLYAEIGPAIMSIFPYNLPGFSYRRALKARIKVEDIVRPVIQKRKKALE 240
             +++ +  LE  Y  +   + ++   N+PGF+Y RALKAR  +    + ++ +R+   +
Sbjct: 206 E-SENVMDALEREYTALNYGVRAM-AVNIPGFAYHRALKARKTLVAAFQSIVTERRNQRK 265

Query: 241 NNVDLEETCQLDAVIKATDKNGEKIFNDNAIICMLLGLLYAGYHTSAHGAQWTMVHVSEN 300
            N+   +   LD ++   D++G K  +D  II +LL  L AG+ +S H   W  V + E+
Sbjct: 266 QNILSNKKDMLDNLLNVKDEDG-KTLDDEEIIDVLLMYLNAGHESSGHTIMWATVFLQEH 325

Query: 301 PDVYQKAKEEQEMIIKQRPPTQKGLNFKEIKQMKYTAKIELIIYSLIIIFFNYCFL---- 360
           P+V Q+AK EQEMI+K RP  QKGL+ KE ++M++ +++  +  +L +I F+        
Sbjct: 326 PEVLQRAKAEQEMILKSRPEGQKGLSLKETRKMEFLSQV--VDETLRVITFSLTAFREAK 385

Query: 361 SSLLIIGYTIPKGWIAAMWIRGVHMDDKIYENPLEFNPSRWDN-YTPKPGEYIPFGIGSR 420
           + + + GY IPKGW    W R VH+D +++ +P +F+P+RWDN + PK G ++PFG GS 
Sbjct: 386 TDVEMNGYLIPKGWKVLTWFRDVHIDPEVFPDPRKFDPARWDNGFVPKAGAFLPFGAGSH 445

Query: 421 FCPGNELAKLEMTVLLHHFLLGYR 437
            CPGN+LAKLE+++ LHHFLL Y+
Sbjct: 446 LCPGNDLAKLEISIFLHHFLLKYQ 461

BLAST of MS005443 vs. TAIR 10
Match: AT5G36110.1 (cytochrome P450, family 716, subfamily A, polypeptide 1 )

HSP 1 Score: 164.5 bits (415), Expect = 2.0e-40
Identity = 116/432 (26.85%), Postives = 212/432 (49.07%), Query Frame = 0

Query: 7   LWYRFRLGKNVYNALPPGDMGWPLVGSSLSFYQSFKIGGDPNSVMDNLISRFGRVR--MY 66
           L  R  L    Y  LPPG+ G PL+G S SF  + +  G P   + + + RF      ++
Sbjct: 20  LLLRKHLSHFSYPNLPPGNTGLPLIGESFSFLSAGR-QGHPEKFITDRVRRFSSSSSCVF 79

Query: 67  KSHLYGFPVIIVTDPEVCRRIYLDDEHFKLN-YPKSV-KLLQADGSLLKVDYKIVHRLMA 126
           K+HL+G P  +VT     + ++ ++    ++ +P SV K+  +       +     R++ 
Sbjct: 80  KTHLFGSPTAVVTGASGNKFLFTNENKLVVSWWPDSVNKIFPSSMQTSSKEEARKLRMLL 139

Query: 127 APINGVEALSRYVEFIEDAVVEGLEEWGSMKGPVPLLHEFKRLTFKIIIGVFLGPVFTDS 186
           +     EAL RYV  +++      E   + +  V +    K+ TF I    FL  +   +
Sbjct: 140 SQFMKPEALRRYVGVMDEIAQRHFETEWANQDQVIVFPLTKKFTFSIACRSFLS-MEDPA 199

Query: 187 EVAELESLYAEIGPAIMSIFPYNLPGFSYRRALKARIKVEDIVRPVIQKRKKALENNVDL 246
            V +LE  +  +   I SI P +LPG  + RA+KA   +   V  ++++RK+ L+    L
Sbjct: 200 RVRQLEEQFNTVAVGIFSI-PIDLPGTRFNRAIKASRLLRKEVSAIVRQRKEELKAGKAL 259

Query: 247 EETCQLDAVIKATDKNGEKIFNDNAIICMLLGLLYAGYHTSAHGAQWTMVHVSENPDVYQ 306
           EE   L  ++    +  ++   D      ++GLL  G+ T++    + + +++E P VYQ
Sbjct: 260 EEHDILSHMLMNIGETKDEDLADK-----IIGLLIGGHDTASIVCTFVVNYLAEFPHVYQ 319

Query: 307 KAKEEQEMIIKQRPPTQKGLNFKEIKQMKYTAKIELIIYSLIIIFFNYC--FLSSLLIIG 366
           +  +EQ+ I+K++   ++GL +++I++M+Y+  +   +  ++          +      G
Sbjct: 320 RVLQEQKEILKEK-KEKEGLRWEDIEKMRYSWNVACEVMRIVPPLSGTFREAIDHFSFKG 379

Query: 367 YTIPKGWIAAMWIRGVHMDDKIYENPLEFNPSRWDNYTPKPGEYIPFGIGSRFCPGNELA 426
           + IPKGW         HM+   +  P  F P+R++   PKP  Y+PFG G R CPG E A
Sbjct: 380 FYIPKGWKLYWSATATHMNPDYFPEPERFEPNRFEGSGPKPYTYVPFGGGPRMCPGKEYA 439

Query: 427 KLEMTVLLHHFL 433
           +LE+ + +H+ +
Sbjct: 440 RLEILIFMHNLV 442

BLAST of MS005443 vs. TAIR 10
Match: AT3G13730.1 (cytochrome P450, family 90, subfamily D, polypeptide 1 )

HSP 1 Score: 163.7 bits (413), Expect = 3.3e-40
Identity = 119/425 (28.00%), Postives = 202/425 (47.53%), Query Frame = 0

Query: 22  PPGDMGWPLVGSSLSFYQSFKIGGDPNSVMDNLISRFGRVRMYKSHLYGFPVIIVTDPEV 81
           P G +GWP++G ++ F  S      P S MD     +GRV  +KSH++G   I+ TD EV
Sbjct: 52  PHGSLGWPVIGETIEFVSS-AYSDRPESFMDKRRLMYGRV--FKSHIFGTATIVSTDAEV 111

Query: 82  CRRIYLDDE-HFKLNYPKSVKLLQADGSLLKVD---YKIVHRLMAAPINGVEALSRYVEF 141
            R +   D   F   YPK+V+ L    S+L ++   ++  H L+ + +      ++ V  
Sbjct: 112 NRAVLQSDSTAFVPFYPKTVRELMGKSSILLINGSLHRRFHGLVGSFLKSPLLKAQIVRD 171

Query: 142 IEDAVVEGLEEWGSMKGPVPLLHEFKRLTFKIIIGVFLGPVFTDSEVAELESLYAEIGPA 201
           +   + E ++ W S   PV L    K + FK++    +  V    ++ EL+  +      
Sbjct: 172 MHKFLSESMDLW-SEDQPVLLQDVSKTVAFKVLAKALIS-VEKGEDLEELKREFENFISG 231

Query: 202 IMSIFPYNLPGFSYRRALKAR----IKVEDIVRPVIQKRKKALENNVDLEETCQLDAVIK 261
           +MS+ P N PG    R+L+A+     +VE I+   I+K K   E++V  ++      V+ 
Sbjct: 232 LMSL-PINFPGTQLHRSLQAKKNMVKQVERIIEGKIRKTKNKEEDDVIAKD------VVD 291

Query: 262 ATDKNGEKIFNDNAIICMLLGLLYAGYHTSAHGAQWTMVHVSENPDVYQKAKEEQEMIIK 321
              K+  +    N I   ++ ++  G+ +        +  +S++P       EE   +  
Sbjct: 292 VLLKDSSEHLTHNLIANNMIDMMIPGHDSVPVLITLAVKFLSDSPAALNLLTEENMKLKS 351

Query: 322 QRPPTQKGLNFKEIKQMKYTAKI--ELIIYSLIIIFFNYCFLSSLLIIGYTIPKGWIAAM 381
            +  T + L + +   + +T K+  E +    +II      +  + I GY IPKGW    
Sbjct: 352 LKELTGEPLYWNDYLSLPFTQKVITETLRMGNVIIGVMRKAMKDVEIKGYVIPKGWCFLA 411

Query: 382 WIRGVHMDDKIYENPLEFNPSRWDNYTPKPGEYIPFGIGSRFCPGNELAKLEMTVLLHHF 437
           ++R VH+D   YE+P +FNP RW         + PFG G R CPG +LA+LE +V LHH 
Sbjct: 412 YLRSVHLDKLYYESPYKFNPWRWQERDMNTSSFSPFGGGQRLCPGLDLARLETSVFLHHL 464