MS005423 (gene) Bitter gourd (TR) v1

BLAST of MS005423 vs. NCBI nr
Match: XP_022159669.1 (nuclear intron maturase 2, mitochondrial [Momordica charantia])

HSP 1 Score: 1466.1 bits (3794), Expect = 0.0e+00
Identity = 721/725 (99.45%), Postives = 722/725 (99.59%), Query Frame = 0

Query: 1   MHRGVSVFAHRIFASSSIVRTSNVFVFSKLNPPNSSFYGFSFCRVLSFVPPHRRTPDPDD 60
           MHRGVSVFAHRIFASSSIVRTSNVFVFSKLNPPNSSFYGFSFCRV SFVPPHRRTPDPDD
Sbjct: 1   MHRGVSVFAHRIFASSSIVRTSNVFVFSKLNPPNSSFYGFSFCRVFSFVPPHRRTPDPDD 60

Query: 61  PSTLMKEDGVSVCSQMWIENFREPDRIVTNLTSYLRRFELWVLAYQKVCADEMGAYMPRN 120
           PSTLMKEDGVSVCSQMWIENFREPDRIVTNLTSYLRRFELWVLAYQKVCADEMGAYMPRN
Sbjct: 61  PSTLMKEDGVSVCSQMWIENFREPDRIVTNLTSYLRRFELWVLAYQKVCADEMGAYMPRN 120

Query: 121 AIQRSALEDLLALRNAVLDNKFKWGARLQFFIKSPKDKTDYESLSKRKIKAILTTTQAAA 180
           AIQRSALEDLLALRNAVLDNKFKWGARLQFFIKSPKDKTDYESLSKRKIKAILTTTQAAA
Sbjct: 121 AIQRSALEDLLALRNAVLDNKFKWGARLQFFIKSPKDKTDYESLSKRKIKAILTTTQAAA 180

Query: 181 FQDKIVQEVLFMILEPIYEARFSPKSYAFRPGRNAHTVLRVIRRHFAGYLWYVKGDLSTI 240
           FQDKIVQEVLFMILEPIYEARFSPKSYAFRPGRNAHTVLRVIRRHFAGYLWYVKGDLSTI
Sbjct: 181 FQDKIVQEVLFMILEPIYEARFSPKSYAFRPGRNAHTVLRVIRRHFAGYLWYVKGDLSTI 240

Query: 241 LDGMKVGLVINALMRDIRDKKVIDLVKSALVTPVITSKVDEGEKKKKKKRKYQKKRVLAE 300
           LDGMKVGLVINALMR IRDKKVIDLVKSALVTPVITSKVDEGEKKKKKKRKYQKKRVLAE
Sbjct: 241 LDGMKVGLVINALMRGIRDKKVIDLVKSALVTPVITSKVDEGEKKKKKKRKYQKKRVLAE 300

Query: 301 DEPKPDPYWLETFFGFAPEEAVKIPSWGHCGILSPLLANICLDELDRWMEGRIKDFYSPS 360
           DEPKPDPYWLETFFGFAPEEAVKIPSWGHCGILSPLLANICLDELDRWMEGRIKDFYSPS
Sbjct: 301 DEPKPDPYWLETFFGFAPEEAVKIPSWGHCGILSPLLANICLDELDRWMEGRIKDFYSPS 360

Query: 361 KSDVIWNSPEGEADQGNTSWPEFVPTSGPDKTRKMDYIRYGGHILIGIRGPRADAATLRK 420
           KSDVIWNSPEGEADQGNTSWPEFVPTSGPDKTRKMDYIRYGGHILIGIRGPRADAATLRK
Sbjct: 361 KSDVIWNSPEGEADQGNTSWPEFVPTSGPDKTRKMDYIRYGGHILIGIRGPRADAATLRK 420

Query: 421 QLIEFCDQKYMLKLDNECLPIEHITKGIMFLDHVLCRRVVYPTLRYTATGGKIISEKGVG 480
           QLIEFCDQKYMLKLDNECLPIEHITKGIMFLDHVLCRRVVYPTLRYTATGGKIISEKGVG
Sbjct: 421 QLIEFCDQKYMLKLDNECLPIEHITKGIMFLDHVLCRRVVYPTLRYTATGGKIISEKGVG 480

Query: 481 TLLSVTASLKQCIKQFRKLNFLKGDRDPDPQPCFRMFHATQAHTNAQMNKFLLTIVEWYR 540
           TLLSVTASLKQCIKQFRKLNFLKGDRDPDPQPCFRMFHATQAHTNAQMNKFLLTIVEWYR
Sbjct: 481 TLLSVTASLKQCIKQFRKLNFLKGDRDPDPQPCFRMFHATQAHTNAQMNKFLLTIVEWYR 540

Query: 541 YADNRKKVVNFCSYILRGSLAKLYAAKYKLRSRAKVYKIGARNLSRPLKEKKGQSPEYHN 600
           YADNRKKVVNFCSYILRGSLAKLYAAKYKLRSRAKVYKIGARNLSRPLKEKKGQSPEYHN
Sbjct: 541 YADNRKKVVNFCSYILRGSLAKLYAAKYKLRSRAKVYKIGARNLSRPLKEKKGQSPEYHN 600

Query: 601 LLRMGLAESIDGLKFTRMTLVPETDYTPLPTNWRPDHEKALIEFIMLEDPRTLEEQLRCI 660
           LLRMGLAESIDGLKFTRMTLVPETDYTPLPTNWRPDHEKALIEFIMLEDPR LEEQLRCI
Sbjct: 601 LLRMGLAESIDGLKFTRMTLVPETDYTPLPTNWRPDHEKALIEFIMLEDPRMLEEQLRCI 660

Query: 661 REEGLVSAQDYISMLVWNYKRNATLDQTPLMNSGDQRLLGSSLDRLGPKAKEVEEHDDAF 720
           REEGLVSAQDYISMLVWNYKRNATLDQTPLMNSGDQRLLGSSLDRLGPKAKEVEEHDDAF
Sbjct: 661 REEGLVSAQDYISMLVWNYKRNATLDQTPLMNSGDQRLLGSSLDRLGPKAKEVEEHDDAF 720

Query: 721 RVAEL 726
           RVAE+
Sbjct: 721 RVAEV 725

BLAST of MS005423 vs. NCBI nr
Match: XP_023542306.1 (nuclear intron maturase 2, mitochondrial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1383.6 bits (3580), Expect = 0.0e+00
Identity = 671/725 (92.55%), Postives = 702/725 (96.83%), Query Frame = 0

Query: 1   MHRGVSVFAHRIFASSSIVRTSNVFVFSKLNPPNSSFYGFSFCRVLSFVPPHRRTPDPDD 60
           MHRGVS+FA RIF SS +V TSN F+FSKLNP N SF+GFS CRV SFVP HRRTPDPDD
Sbjct: 1   MHRGVSIFARRIFLSSRVVGTSNGFLFSKLNPLNPSFHGFSVCRVFSFVPAHRRTPDPDD 60

Query: 61  PSTLMKEDGVSVCSQMWIENFREPDRIVTNLTSYLRRFELWVLAYQKVCADEMGAYMPRN 120
           PSTLMKEDGVSVCSQMWIENFREPDRI++NL++YLRRFELWVLAYQKVCADEMGAYMPRN
Sbjct: 61  PSTLMKEDGVSVCSQMWIENFREPDRIISNLSTYLRRFELWVLAYQKVCADEMGAYMPRN 120

Query: 121 AIQRSALEDLLALRNAVLDNKFKWGARLQFFIKSPKDKTDYESLSKRKIKAILTTTQAAA 180
           AIQRSALEDLLALRNAVLD+KFKWGARL+FFIKSPKDKTDYESLSKRKIKAILTTTQ AA
Sbjct: 121 AIQRSALEDLLALRNAVLDSKFKWGARLEFFIKSPKDKTDYESLSKRKIKAILTTTQPAA 180

Query: 181 FQDKIVQEVLFMILEPIYEARFSPKSYAFRPGRNAHTVLRVIRRHFAGYLWYVKGDLSTI 240
           FQDKIVQEVLFMILEPIYEARFSPKSYAFRPGRNAHTVLRVIRRHFAGYLWYVKGDLSTI
Sbjct: 181 FQDKIVQEVLFMILEPIYEARFSPKSYAFRPGRNAHTVLRVIRRHFAGYLWYVKGDLSTI 240

Query: 241 LDGMKVGLVINALMRDIRDKKVIDLVKSALVTPVITSKVDEGEKKKKKKRKYQKKRVLAE 300
           LDGMKVG+VINALMRDIRDKKVIDLVK+ALVTPVITSK+DEGEKKKKKKRKYQKKRVLA+
Sbjct: 241 LDGMKVGMVINALMRDIRDKKVIDLVKAALVTPVITSKIDEGEKKKKKKRKYQKKRVLAD 300

Query: 301 DEPKPDPYWLETFFGFAPEEAVKIPSWGHCGILSPLLANICLDELDRWMEGRIKDFYSPS 360
           DEPKPDPYWLETFFGFAPEEAVK PSWGHCGILSPLLANICLDELD WMEGRIKDFYSPS
Sbjct: 301 DEPKPDPYWLETFFGFAPEEAVKNPSWGHCGILSPLLANICLDELDHWMEGRIKDFYSPS 360

Query: 361 KSDVIWNSPEGEADQGNTSWPEFVPTSGPDKTRKMDYIRYGGHILIGIRGPRADAATLRK 420
           KSDVIWNSPEGEADQGNTSWPEFVPTSGPDKTRKMDY+RYGGHILIGIRGPRADAATLRK
Sbjct: 361 KSDVIWNSPEGEADQGNTSWPEFVPTSGPDKTRKMDYVRYGGHILIGIRGPRADAATLRK 420

Query: 421 QLIEFCDQKYMLKLDNECLPIEHITKGIMFLDHVLCRRVVYPTLRYTATGGKIISEKGVG 480
           QLIEFCDQKYMLKLDNECLPIEHITKGIMFLDHVLCRRVVYPTLRYTATGGKIISEKGVG
Sbjct: 421 QLIEFCDQKYMLKLDNECLPIEHITKGIMFLDHVLCRRVVYPTLRYTATGGKIISEKGVG 480

Query: 481 TLLSVTASLKQCIKQFRKLNFLKGDRDPDPQPCFRMFHATQAHTNAQMNKFLLTIVEWYR 540
           TLLSVTASLKQCIKQFRKLNFLKGDRDPDPQPCFRMFHATQAHTN+QMNKFLLTIVEWYR
Sbjct: 481 TLLSVTASLKQCIKQFRKLNFLKGDRDPDPQPCFRMFHATQAHTNSQMNKFLLTIVEWYR 540

Query: 541 YADNRKKVVNFCSYILRGSLAKLYAAKYKLRSRAKVYKIGARNLSRPLKEKKGQSPEYHN 600
           YADNR+KVVNFCSYILRGSLAKLYAAKYKLRSRAKVYKIGARNLSRPLKEKKGQSPEYHN
Sbjct: 541 YADNRRKVVNFCSYILRGSLAKLYAAKYKLRSRAKVYKIGARNLSRPLKEKKGQSPEYHN 600

Query: 601 LLRMGLAESIDGLKFTRMTLVPETDYTPLPTNWRPDHEKALIEFIMLEDPRTLEEQLRCI 660
           LLRMGLAESIDGLKFTRM+LVPETDYTPLP NWRPDHEKAL+EFIMLEDP+TLEE  RCI
Sbjct: 601 LLRMGLAESIDGLKFTRMSLVPETDYTPLPNNWRPDHEKALLEFIMLEDPKTLEEHRRCI 660

Query: 661 REEGLVSAQDYISMLVWNYKRNATLDQTPLMNSGDQRLLGSSLDRLGPKAKEVEEHDDAF 720
           RE+GLVS QDYISMLVWNYKRNAT+DQT LMN+ D R+LGS+L+ LG KAKE+EEHD++F
Sbjct: 661 REQGLVSPQDYISMLVWNYKRNATMDQTSLMNTIDHRILGSNLESLGSKAKELEEHDESF 720

Query: 721 RVAEL 726
           + AE+
Sbjct: 721 QAAEV 725

BLAST of MS005423 vs. NCBI nr
Match: KAG7033617.1 (Nuclear intron maturase 2, mitochondrial, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1383.2 bits (3579), Expect = 0.0e+00
Identity = 672/725 (92.69%), Postives = 701/725 (96.69%), Query Frame = 0

Query: 1   MHRGVSVFAHRIFASSSIVRTSNVFVFSKLNPPNSSFYGFSFCRVLSFVPPHRRTPDPDD 60
           MHRGVS+FA RIF SS +V TSN F+FSKLNP + SF+GFS CRV SFVP HRRTPDPDD
Sbjct: 1   MHRGVSIFARRIFLSSRVVGTSNGFLFSKLNPLSPSFHGFSVCRVFSFVPAHRRTPDPDD 60

Query: 61  PSTLMKEDGVSVCSQMWIENFREPDRIVTNLTSYLRRFELWVLAYQKVCADEMGAYMPRN 120
           PSTLMKEDGVSVCSQMWIENFREPDRI++NLT+YLRRFELWVLAYQKVCADEMGAYMPRN
Sbjct: 61  PSTLMKEDGVSVCSQMWIENFREPDRIISNLTTYLRRFELWVLAYQKVCADEMGAYMPRN 120

Query: 121 AIQRSALEDLLALRNAVLDNKFKWGARLQFFIKSPKDKTDYESLSKRKIKAILTTTQAAA 180
           AIQRSALEDLLALRNAVLD+KFKWGARL+FFIKSPKDKTDYESLSKRKIKAILTTTQ AA
Sbjct: 121 AIQRSALEDLLALRNAVLDSKFKWGARLEFFIKSPKDKTDYESLSKRKIKAILTTTQPAA 180

Query: 181 FQDKIVQEVLFMILEPIYEARFSPKSYAFRPGRNAHTVLRVIRRHFAGYLWYVKGDLSTI 240
           FQDKIVQEVLFMILEPIYEARFSPKSYAFRPGRNAHTVLRVIRRHFAGYLWYVKGDLSTI
Sbjct: 181 FQDKIVQEVLFMILEPIYEARFSPKSYAFRPGRNAHTVLRVIRRHFAGYLWYVKGDLSTI 240

Query: 241 LDGMKVGLVINALMRDIRDKKVIDLVKSALVTPVITSKVDEGEKKKKKKRKYQKKRVLAE 300
           LDGMKVG+VINALMRDIRDKKVIDLVK+ALVTPVITSK+DEGEKKKKKKRKYQKKRVLA+
Sbjct: 241 LDGMKVGMVINALMRDIRDKKVIDLVKAALVTPVITSKIDEGEKKKKKKRKYQKKRVLAD 300

Query: 301 DEPKPDPYWLETFFGFAPEEAVKIPSWGHCGILSPLLANICLDELDRWMEGRIKDFYSPS 360
           DEPKPDPYWLETFFGFAPEEAVK PSWGHCGILSPLLANICLDELD WMEGRIKDFYSPS
Sbjct: 301 DEPKPDPYWLETFFGFAPEEAVKNPSWGHCGILSPLLANICLDELDHWMEGRIKDFYSPS 360

Query: 361 KSDVIWNSPEGEADQGNTSWPEFVPTSGPDKTRKMDYIRYGGHILIGIRGPRADAATLRK 420
           KSDVIWNSPEGEADQGNTSWPEFVPTSGPDKTRKMDY+RYGGHILIGIRGPRADAATLRK
Sbjct: 361 KSDVIWNSPEGEADQGNTSWPEFVPTSGPDKTRKMDYVRYGGHILIGIRGPRADAATLRK 420

Query: 421 QLIEFCDQKYMLKLDNECLPIEHITKGIMFLDHVLCRRVVYPTLRYTATGGKIISEKGVG 480
           QLIEFCDQKYMLKLDNECLPIEHITKGIMFLDHVLCRRVVYPTLRYTATGGKIISEKGVG
Sbjct: 421 QLIEFCDQKYMLKLDNECLPIEHITKGIMFLDHVLCRRVVYPTLRYTATGGKIISEKGVG 480

Query: 481 TLLSVTASLKQCIKQFRKLNFLKGDRDPDPQPCFRMFHATQAHTNAQMNKFLLTIVEWYR 540
           TLLSVTASLKQCIKQFRKLNFLKGDRDPDPQPCFRMFHATQAHTN+QMNKFLLTIVEWYR
Sbjct: 481 TLLSVTASLKQCIKQFRKLNFLKGDRDPDPQPCFRMFHATQAHTNSQMNKFLLTIVEWYR 540

Query: 541 YADNRKKVVNFCSYILRGSLAKLYAAKYKLRSRAKVYKIGARNLSRPLKEKKGQSPEYHN 600
           YADNR+KVVNFCSYILRGSLAKLYAAKYKLRSRAKVYKIGARNLSRPLKEKKGQSPEYHN
Sbjct: 541 YADNRRKVVNFCSYILRGSLAKLYAAKYKLRSRAKVYKIGARNLSRPLKEKKGQSPEYHN 600

Query: 601 LLRMGLAESIDGLKFTRMTLVPETDYTPLPTNWRPDHEKALIEFIMLEDPRTLEEQLRCI 660
           LLRMGLAESIDGLKFTRM+LVPETDYTPLP NWRPDHEKAL+EFIMLEDPRTLEE  RCI
Sbjct: 601 LLRMGLAESIDGLKFTRMSLVPETDYTPLPNNWRPDHEKALLEFIMLEDPRTLEEHRRCI 660

Query: 661 REEGLVSAQDYISMLVWNYKRNATLDQTPLMNSGDQRLLGSSLDRLGPKAKEVEEHDDAF 720
           RE+GLVS QDYISMLVWNYKRNAT+DQT LMN+ D R+LGS+L  LG KAKE+EEHD++F
Sbjct: 661 REQGLVSPQDYISMLVWNYKRNATMDQTSLMNTIDHRILGSNLGSLGSKAKELEEHDESF 720

Query: 721 RVAEL 726
           + AE+
Sbjct: 721 QAAEV 725

BLAST of MS005423 vs. NCBI nr
Match: KAG6571755.1 (Nuclear intron maturase 2, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1382.9 bits (3578), Expect = 0.0e+00
Identity = 672/725 (92.69%), Postives = 700/725 (96.55%), Query Frame = 0

Query: 1   MHRGVSVFAHRIFASSSIVRTSNVFVFSKLNPPNSSFYGFSFCRVLSFVPPHRRTPDPDD 60
           MHRGVS+FA RIF SS +V TSN F+FSKLNP + SF+GFS CRV SFVP HRRTPDPDD
Sbjct: 1   MHRGVSIFARRIFLSSRVVGTSNGFLFSKLNPLSPSFHGFSVCRVFSFVPAHRRTPDPDD 60

Query: 61  PSTLMKEDGVSVCSQMWIENFREPDRIVTNLTSYLRRFELWVLAYQKVCADEMGAYMPRN 120
           PSTLMKEDGVSVCSQMWIENFREPDRI++NLT+YLRRFELWVLAYQKVCADEMGAYMPRN
Sbjct: 61  PSTLMKEDGVSVCSQMWIENFREPDRIISNLTTYLRRFELWVLAYQKVCADEMGAYMPRN 120

Query: 121 AIQRSALEDLLALRNAVLDNKFKWGARLQFFIKSPKDKTDYESLSKRKIKAILTTTQAAA 180
           AIQRSALEDLLALRNAVLD+KFKWGARL+FFIKSPKDKTDYESLSKRKIKAILTTTQ AA
Sbjct: 121 AIQRSALEDLLALRNAVLDSKFKWGARLEFFIKSPKDKTDYESLSKRKIKAILTTTQPAA 180

Query: 181 FQDKIVQEVLFMILEPIYEARFSPKSYAFRPGRNAHTVLRVIRRHFAGYLWYVKGDLSTI 240
           FQDKIVQEVLFMILEPIYEARFSPKSYAFRPGRNAHTVLRVIRRHFAGYLWYVKGDLSTI
Sbjct: 181 FQDKIVQEVLFMILEPIYEARFSPKSYAFRPGRNAHTVLRVIRRHFAGYLWYVKGDLSTI 240

Query: 241 LDGMKVGLVINALMRDIRDKKVIDLVKSALVTPVITSKVDEGEKKKKKKRKYQKKRVLAE 300
           LDGMKVG+VINALMRDIRDKKVIDLVK+ALVTPVITSK+DEGEKKKKKKRKYQKKRVLA+
Sbjct: 241 LDGMKVGMVINALMRDIRDKKVIDLVKAALVTPVITSKIDEGEKKKKKKRKYQKKRVLAD 300

Query: 301 DEPKPDPYWLETFFGFAPEEAVKIPSWGHCGILSPLLANICLDELDRWMEGRIKDFYSPS 360
           DEPKPDPYWLETFFGFAPEEAVK PSWGHCGILSPLLANICLDELD WMEGRIKDFYSPS
Sbjct: 301 DEPKPDPYWLETFFGFAPEEAVKNPSWGHCGILSPLLANICLDELDHWMEGRIKDFYSPS 360

Query: 361 KSDVIWNSPEGEADQGNTSWPEFVPTSGPDKTRKMDYIRYGGHILIGIRGPRADAATLRK 420
           KSDVIWNSPEGEADQGNTSWPEFVPTSGPDKTRKMDY+RYGGHILIGIRGPRADAATLRK
Sbjct: 361 KSDVIWNSPEGEADQGNTSWPEFVPTSGPDKTRKMDYVRYGGHILIGIRGPRADAATLRK 420

Query: 421 QLIEFCDQKYMLKLDNECLPIEHITKGIMFLDHVLCRRVVYPTLRYTATGGKIISEKGVG 480
           QLIEFCDQKYMLKLDNECLPIEHITKGIMFLDHVLCRRVVYPTLRYTATGGKIISEKGVG
Sbjct: 421 QLIEFCDQKYMLKLDNECLPIEHITKGIMFLDHVLCRRVVYPTLRYTATGGKIISEKGVG 480

Query: 481 TLLSVTASLKQCIKQFRKLNFLKGDRDPDPQPCFRMFHATQAHTNAQMNKFLLTIVEWYR 540
           TLLSVTASLKQCIKQFRKLNFLKGDRDPDPQPCFRMFHATQAHTN+QMNKFLLTIVEWYR
Sbjct: 481 TLLSVTASLKQCIKQFRKLNFLKGDRDPDPQPCFRMFHATQAHTNSQMNKFLLTIVEWYR 540

Query: 541 YADNRKKVVNFCSYILRGSLAKLYAAKYKLRSRAKVYKIGARNLSRPLKEKKGQSPEYHN 600
           YADNR+KVVNFCSYILRGSLAKLYAAKYKLRSRAKVYKIGARNLSRPLKEKKGQSPEYHN
Sbjct: 541 YADNRRKVVNFCSYILRGSLAKLYAAKYKLRSRAKVYKIGARNLSRPLKEKKGQSPEYHN 600

Query: 601 LLRMGLAESIDGLKFTRMTLVPETDYTPLPTNWRPDHEKALIEFIMLEDPRTLEEQLRCI 660
           LLRMGLAESIDGLKFTRM+LVPETDYTPLP NWRPDHEKAL+EFIMLEDPRTLEE  RCI
Sbjct: 601 LLRMGLAESIDGLKFTRMSLVPETDYTPLPNNWRPDHEKALLEFIMLEDPRTLEEHRRCI 660

Query: 661 REEGLVSAQDYISMLVWNYKRNATLDQTPLMNSGDQRLLGSSLDRLGPKAKEVEEHDDAF 720
           RE+GLVS QDYISMLVWNYKRNAT+DQT LMN+ D R+LGS+L  LG KAKE+EEHD++F
Sbjct: 661 REQGLVSPQDYISMLVWNYKRNATMDQTSLMNTIDHRILGSNLGSLGSKAKELEEHDESF 720

Query: 721 RVAEL 726
             AE+
Sbjct: 721 HAAEV 725

BLAST of MS005423 vs. NCBI nr
Match: XP_022932969.1 (nuclear intron maturase 2, mitochondrial [Cucurbita moschata])

HSP 1 Score: 1380.2 bits (3571), Expect = 0.0e+00
Identity = 669/725 (92.28%), Postives = 701/725 (96.69%), Query Frame = 0

Query: 1   MHRGVSVFAHRIFASSSIVRTSNVFVFSKLNPPNSSFYGFSFCRVLSFVPPHRRTPDPDD 60
           MHRGVS+FA RIF SS +V TSN F+FSKLNP + SF+GFS CRV SFVP HRRTPDPDD
Sbjct: 1   MHRGVSIFARRIFLSSRVVGTSNGFLFSKLNPLSPSFHGFSVCRVFSFVPAHRRTPDPDD 60

Query: 61  PSTLMKEDGVSVCSQMWIENFREPDRIVTNLTSYLRRFELWVLAYQKVCADEMGAYMPRN 120
           PSTLMKEDGVSVCSQMWIENFREPDRI++NLT+YLRRFELWVLAYQKVCADEMGAYMPRN
Sbjct: 61  PSTLMKEDGVSVCSQMWIENFREPDRIISNLTTYLRRFELWVLAYQKVCADEMGAYMPRN 120

Query: 121 AIQRSALEDLLALRNAVLDNKFKWGARLQFFIKSPKDKTDYESLSKRKIKAILTTTQAAA 180
           AIQRSALEDLLALRNAVLD+KFKWGARL+FFIKSPKDKTDYESLSKRKIKAILTTTQ AA
Sbjct: 121 AIQRSALEDLLALRNAVLDSKFKWGARLEFFIKSPKDKTDYESLSKRKIKAILTTTQPAA 180

Query: 181 FQDKIVQEVLFMILEPIYEARFSPKSYAFRPGRNAHTVLRVIRRHFAGYLWYVKGDLSTI 240
           FQDKIVQEVLFMILEPIYEARFSPKSYAFRPGRNAHTVLR+IRRHFAGYLWYVKGDLSTI
Sbjct: 181 FQDKIVQEVLFMILEPIYEARFSPKSYAFRPGRNAHTVLRIIRRHFAGYLWYVKGDLSTI 240

Query: 241 LDGMKVGLVINALMRDIRDKKVIDLVKSALVTPVITSKVDEGEKKKKKKRKYQKKRVLAE 300
           LDGMKVG+VINALMRDIRDKKVIDLVK+ALVTPVITSK+DEGEKKKKKKRKYQKKRVLA+
Sbjct: 241 LDGMKVGMVINALMRDIRDKKVIDLVKAALVTPVITSKIDEGEKKKKKKRKYQKKRVLAD 300

Query: 301 DEPKPDPYWLETFFGFAPEEAVKIPSWGHCGILSPLLANICLDELDRWMEGRIKDFYSPS 360
           DEPKPDPYWLETFFGFAPEEAVK PSWGHCGILSPLLANICLDELD WMEGRIKDFY+PS
Sbjct: 301 DEPKPDPYWLETFFGFAPEEAVKNPSWGHCGILSPLLANICLDELDHWMEGRIKDFYAPS 360

Query: 361 KSDVIWNSPEGEADQGNTSWPEFVPTSGPDKTRKMDYIRYGGHILIGIRGPRADAATLRK 420
           KSDVIWNSPEGEADQGNTSWPEFVPTSGPDKTRKMDY+RYGGHILIGIRGPRADAATLRK
Sbjct: 361 KSDVIWNSPEGEADQGNTSWPEFVPTSGPDKTRKMDYVRYGGHILIGIRGPRADAATLRK 420

Query: 421 QLIEFCDQKYMLKLDNECLPIEHITKGIMFLDHVLCRRVVYPTLRYTATGGKIISEKGVG 480
           QLIEFCDQKYMLKLDNECLPIEHITKGIMFLDHVLCRRVVYPTLRYTATGGKIISEKGVG
Sbjct: 421 QLIEFCDQKYMLKLDNECLPIEHITKGIMFLDHVLCRRVVYPTLRYTATGGKIISEKGVG 480

Query: 481 TLLSVTASLKQCIKQFRKLNFLKGDRDPDPQPCFRMFHATQAHTNAQMNKFLLTIVEWYR 540
           TLLSVTASLKQCIKQFRKLNFLKGDRDPDPQPCFRMFHATQAHTN+QMNKFLLTIVEWYR
Sbjct: 481 TLLSVTASLKQCIKQFRKLNFLKGDRDPDPQPCFRMFHATQAHTNSQMNKFLLTIVEWYR 540

Query: 541 YADNRKKVVNFCSYILRGSLAKLYAAKYKLRSRAKVYKIGARNLSRPLKEKKGQSPEYHN 600
           YADNR+KVVNFCSYILRGSLAKLYAAKYKLRSRAKVYKIGARNLSRPLKEKKGQSPEYHN
Sbjct: 541 YADNRRKVVNFCSYILRGSLAKLYAAKYKLRSRAKVYKIGARNLSRPLKEKKGQSPEYHN 600

Query: 601 LLRMGLAESIDGLKFTRMTLVPETDYTPLPTNWRPDHEKALIEFIMLEDPRTLEEQLRCI 660
           LLRMGLAESIDGLKFTRM+LVPETDYTPLP NWRPDHEKAL+EFIMLEDPRTLEE  RCI
Sbjct: 601 LLRMGLAESIDGLKFTRMSLVPETDYTPLPNNWRPDHEKALLEFIMLEDPRTLEEHRRCI 660

Query: 661 REEGLVSAQDYISMLVWNYKRNATLDQTPLMNSGDQRLLGSSLDRLGPKAKEVEEHDDAF 720
           RE+GLVS QDYISMLVWNYKRNA++DQT LMN+ D R+LGS+L  LG KAKE+EEHD++F
Sbjct: 661 REQGLVSPQDYISMLVWNYKRNASMDQTSLMNTIDHRILGSNLGSLGSKAKELEEHDESF 720

Query: 721 RVAEL 726
           + AE+
Sbjct: 721 QAAEV 725

BLAST of MS005423 vs. ExPASy Swiss-Prot
Match: Q9FJR9 (Nuclear intron maturase 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=NMAT2 PE=1 SV=1)

HSP 1 Score: 1141.7 bits (2952), Expect = 0.0e+00
Identity = 567/738 (76.83%), Postives = 634/738 (85.91%), Query Frame = 0

Query: 1   MHRGVSVFAHRIFASSSIVRTSNVFVFSKLNPP--NSSFYGFSFCRVLSFVPPHRR---- 60
           M R  SV     +   S   T NV+ F   NPP  ++   G +  R LS+  P +R    
Sbjct: 1   MRRSFSVLGPYKWLKPSSTYT-NVYCF-VTNPPLISNPNNGSAIFRYLSYFAPQQRRQQQ 60

Query: 61  -TPDPDDPSTLMKEDGVSVCSQMWIENFREPDRIVTNLTSYLRRFELWVLAYQKVCADEM 120
             PDPDDP+ L+KEDGVS+CSQMW+ENF+EPD+  TNLTSYLRRFELWVLAYQKVC DE+
Sbjct: 61  QAPDPDDPANLLKEDGVSLCSQMWLENFKEPDKTATNLTSYLRRFELWVLAYQKVCCDEL 120

Query: 121 GAYMPRNAIQRSALEDLLALRNAVLDNKFKWGARLQFFIKSPKDKTDYESLSKRKIKAIL 180
           GAY+PR++IQRSALE+LLALRN+VLD++FKWG+RL F+IKSP+DKTDYESLSKRKIKAIL
Sbjct: 121 GAYVPRSSIQRSALENLLALRNSVLDDRFKWGSRLDFYIKSPRDKTDYESLSKRKIKAIL 180

Query: 181 TTTQAAAFQDKIVQEVLFMILEPIYEARFSPKSYAFRPGRNAHTVLRVIRRHFAGYLWYV 240
           TTTQ   FQD+IVQEVL MILEPIYE+RFS KS+AFRPGR AHTVLRVIRR+FAGYLWYV
Sbjct: 181 TTTQPTPFQDRIVQEVLLMILEPIYESRFSQKSFAFRPGRTAHTVLRVIRRNFAGYLWYV 240

Query: 241 KGDLSTILDGMKVGLVINALMRDIRDKKVIDLVKSALVTPVITSKVDEGEKKKKKKRKYQ 300
           KGDLS +LDGMKVG VI++LMRD+RDKKVIDL+KSALVTPV+TSKV++GEKKK KKRKYQ
Sbjct: 241 KGDLSVVLDGMKVGFVISSLMRDVRDKKVIDLIKSALVTPVVTSKVEDGEKKKTKKRKYQ 300

Query: 301 KKRVLAEDEPKPDPYWLETFFGFAPEEAVKIPSWGHCGILSPLLANICLDELDRWMEGRI 360
           KKRVLAEDEPKPDPYWLETFFGFAPEEA K P WGHCGILSPLL N+CLDELDRWME ++
Sbjct: 301 KKRVLAEDEPKPDPYWLETFFGFAPEEAGKSPQWGHCGILSPLLVNVCLDELDRWMETKV 360

Query: 361 KDFYSPSKSDVIWNSPEGEADQGNTSWPEFVPTSGPDKTRKMDYIRYGGHILIGIRGPRA 420
           KDFY PSKSDVIWN+PEGEADQGNTSWPEFVPTSGPDKTRKMDY+RYGGHILIG+RGPRA
Sbjct: 361 KDFYRPSKSDVIWNNPEGEADQGNTSWPEFVPTSGPDKTRKMDYVRYGGHILIGVRGPRA 420

Query: 421 DAATLRKQLIEFCDQKYMLKLDNECLPIEHITKGIMFLDHVLCRRVVYPTLRYTATGGKI 480
           DAATLRK+LIEF DQKYML+LDNE LPIEHITKGIMFLDHVLCRRVVYPTLRYTATGGKI
Sbjct: 421 DAATLRKELIEFVDQKYMLRLDNENLPIEHITKGIMFLDHVLCRRVVYPTLRYTATGGKI 480

Query: 481 ISEKGVGTLLSVTASLKQCIKQFRKLNFLKGDRDPDPQPCFRMFHATQAHTNAQMNKFLL 540
           ISEKGVGTLLSVTASLKQCIKQFRKL F+KGDRDPDPQPCFRMFHATQAHTN QMNKFL 
Sbjct: 481 ISEKGVGTLLSVTASLKQCIKQFRKLLFIKGDRDPDPQPCFRMFHATQAHTNNQMNKFLT 540

Query: 541 TIVEWYRYADNRKKVVNFCSYILRGSLAKLYAAKYKLRSRAKVYKIGARNLSRPLKEKKG 600
           TI EWYR+ADNRKK+VNFCSYI+RGSLAKLYAAKYKLRSRAKVYK   RNLS PL +KKG
Sbjct: 541 TIAEWYRFADNRKKIVNFCSYIIRGSLAKLYAAKYKLRSRAKVYKFANRNLSLPLLQKKG 600

Query: 601 QSPEYHNLLRMGLAESIDGLKFTRMTLVPETDYTPLPTNWRPDHEKALIEFIMLEDPRTL 660
           QSPEY NLLRMGLAES+DGL +TRM+LVPETDY+P P NWRP+HEK LIE++ L++P+TL
Sbjct: 601 QSPEYQNLLRMGLAESVDGLVYTRMSLVPETDYSPFPGNWRPEHEKFLIEYLTLDEPKTL 660

Query: 661 EEQLRCIREEGLVSAQDYISMLVWNYKRNA-TLDQTPLMNSGDQRLLGSS----LDRLGP 720
           EEQ R IRE+GLVS QDY SMLVWNYKRNA  +DQ  ++      LLGSS     D    
Sbjct: 661 EEQKRFIREKGLVSPQDYTSMLVWNYKRNAIPMDQVSILKD-QPFLLGSSSTYNRDNDDQ 720

Query: 721 KAKEV-EEHDDAFRVAEL 726
           K KE  E+ +D  R+A +
Sbjct: 721 KNKEEDEDSEDGLRIARM 735

BLAST of MS005423 vs. ExPASy Swiss-Prot
Match: Q9C8R8 (Nuclear intron maturase 1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=NMAT1 PE=1 SV=1)

HSP 1 Score: 729.6 bits (1882), Expect = 4.0e-209
Identity = 348/647 (53.79%), Postives = 482/647 (74.50%), Query Frame = 0

Query: 53  RRTPDPDDPSTLMKEDGVSVCSQMWIENFRE-PDRIVTNLTSYLRRFELWVLAYQKVCAD 112
           R      DP +L+K+D V +C  +W+++F   P    +NLT +L +F+LWVLAYQ+ CA 
Sbjct: 41  REPSSTQDPYSLLKQDPVDICLSLWVKSFSSPPSATFSNLTGFLSKFDLWVLAYQRTCAH 100

Query: 113 EMGAYMPRNAIQRSALEDLLALRNAVL--DNKFKWGARLQFFIKSPKDKTDY---ESLSK 172
             G + PRNAI  +AL  LL+L+NAV     KF+W  ++  +++SPKDK      E +SK
Sbjct: 101 VTGTFPPRNAIHANALRSLLSLQNAVTRSGGKFRWNDKMNQYVRSPKDKISMNGGEGMSK 160

Query: 173 RKIKAILTTTQAAAFQDKIVQEVLFMILEPIYEARFSPKSYAFRPGRNAHTVLRVIRRHF 232
            K++ I+  ++   FQD++V EVL MILEP +EARFS KS+ FRPGRN HTV+R IR +F
Sbjct: 161 GKVRRII-ESEEPIFQDRVVHEVLLMILEPFFEARFSSKSHGFRPGRNPHTVIRTIRSNF 220

Query: 233 AGYLWYVKGDLSTILDGMKVGLVINALMRDIRDKKVIDLVKSALV---TPVITSKVDE-- 292
           AGYLW++KGD+S +LD + V +V+N L + ++D+KV+ L++S+L      V+   V++  
Sbjct: 221 AGYLWFMKGDVSEMLDHVDVDVVMNCLQKVVKDRKVLGLIESSLKFSDKRVLKRVVEKHG 280

Query: 293 -----GEKKK--KKKRKYQKKRVLAEDEPKPDPYWLETFFGFAPEEAVKIPSWGHCGILS 352
                G K++  ++KR   KK++L++DEPKPDPYWL TF+ FAP+EA K+PS+G+CG+LS
Sbjct: 281 NDNGLGTKRRIEREKRNKTKKKILSDDEPKPDPYWLRTFYSFAPKEAAKVPSYGYCGVLS 340

Query: 353 PLLANICLDELDRWMEGRIKDFYSPSKSDVIWNSPEGEADQGNTSWPEFVPTSGPDKTRK 412
           PLLAN+CL+ELDR+ME +I +++SP K D IW     E    N +WPEFVP+SG +KTRK
Sbjct: 341 PLLANVCLNELDRFMETKIVEYFSPCKDDSIWKE-SIEDGCHNPAWPEFVPSSGKEKTRK 400

Query: 413 MDYIRYGGHILIGIRGPRADAATLRKQLIEFCDQKYMLKLDNECLPIEHITKGIMFLDHV 472
           MDYIRYGGH LIGIRGPR DA  +RK++I+FCD+ + ++LDN  L IEHI++GI FLDH+
Sbjct: 401 MDYIRYGGHFLIGIRGPREDAVKMRKEIIDFCDRVFGVRLDNSKLEIEHISRGIQFLDHI 460

Query: 473 LCRRVVYPTLRYTATGGKIISEKGVGTLLSVTASLKQCIKQFRKLNFLKGDRDPDPQPCF 532
           +CRRV+YPTLRYT +GG I+S+KGVGTLLSV+ASL+QCI+QFR+L F+KGD+DP+P PC 
Sbjct: 461 ICRRVIYPTLRYTGSGGSIVSKKGVGTLLSVSASLEQCIRQFRRLAFVKGDKDPEPLPCN 520

Query: 533 RMFHATQAHTNAQMNKFLLTIVEWYRYADNRKKVVNFCSYILRGSLAKLYAAKYKLRSRA 592
            M +++Q+H+N+QMNKFL T+ +WY+YADNRKK V FC+Y++R SLAKLYAA+Y+L+SRA
Sbjct: 521 PMLYSSQSHSNSQMNKFLETMADWYKYADNRKKAVGFCAYVIRSSLAKLYAARYRLKSRA 580

Query: 593 KVYKIGARNLSRPLKEKKGQS-PEYHNLLRMGLAESIDGLKFTRMTLVPETDYTPLPTNW 652
           KVY I +R+LS PL E    S PEY +LLRMGL ++I+G++F+RM+L+P  DYTP P NW
Sbjct: 581 KVYSIASRDLSHPLSESSNNSAPEYSDLLRMGLVDAIEGVQFSRMSLIPSCDYTPFPRNW 640

Query: 653 RPDHEKALIEFIMLEDPRTLEEQLRCIREEGLVSAQDYISMLVWNYK 681
            P+HE+ L E+I L+DP+      R I+ EGL   QD IS  VW++K
Sbjct: 641 IPNHEQVLQEYIRLQDPKFFCGLHRSIKREGLTLPQDEISEAVWDFK 685

BLAST of MS005423 vs. ExPASy Swiss-Prot
Match: P38456 (Uncharacterized mitochondrial protein ymf11 OS=Marchantia polymorpha OX=3197 GN=YMF11 PE=4 SV=1)

HSP 1 Score: 154.5 bits (389), Expect = 5.4e-36
Identity = 134/524 (25.57%), Postives = 217/524 (41.41%), Query Frame = 0

Query: 182 QDKIVQEVLFMILEPIYEARFSPKSYAFRPGRNAHTVLRVIRRHFAGYLWYVKGDLSTIL 241
           +D +VQEV+  ILE +YE  F   S+ FRPGR+ HT L+ IRR F G +W+++G+ S   
Sbjct: 243 KDILVQEVIRSILETLYEPYFLSCSHGFRPGRSQHTCLKQIRRDFVGTVWFIEGETSQYF 302

Query: 242 DGMKVGLVINALMRDIRDKKVIDLVKSALVTPVITSKVDEGEKKKKKKRKYQKKRVLAED 301
           + +   ++I  + R IRD + ++LV+  + T +       G                   
Sbjct: 303 NKIDKQVLIGLMRRRIRDNRFLNLVQKEIKTSLRAGAEGTG------------------- 362

Query: 302 EPKPDPYWLETFFGFAPEEAVKIPSWGHCGILSPLLANICLDELDRWM--------EGRI 361
                                          + PLL NI L ELD ++         GR 
Sbjct: 363 -------------------------------VGPLLCNIVLHELDLFVMRLKRIVDRGRR 422

Query: 362 KDFYSPSKSDVIWNSPEGEADQGNTSWPEFVPTSG--------PDKTRKMDYIRYGGHIL 421
           +     SK   +W       D+           SG        P +TR+++Y+R+    L
Sbjct: 423 RAVNPESKE--LWRQSAALIDRTTAHRARVPFPSGAFGRGLGHPQETRQINYVRFADDFL 482

Query: 422 IGIRGPRADAATLRKQLIEFCDQKYMLKLDNE-----------CLPIEHITKG------- 481
           IG+ GPRA A  +R  +  F + +  L+L  +            LP  ++  G       
Sbjct: 483 IGVIGPRALAERIRGLVTRFIEVRLKLRLTLDKTRKPIQSRPNTLPAHYVPMGPKETGPK 542

Query: 482 --------------------------IMFLDHVLCRRVVYPTLRYTATGGKIISEKGVGT 541
                                     I FL +++ R   + T      G +    +  G 
Sbjct: 543 VENDFTISGGPGKKKTQIFCITKNKKIPFLGYLISRDSKH-TYNLVRRGRRYRIRRSGG- 602

Query: 542 LLSVTASLKQCIKQFRKLNFLKGDRDPDPQPCFRMFHATQAHTNAQMNKFLLTIVEWYRY 601
            LS+   +++ I +  +  F   D+   P+P F  F   Q+++ A++   L  +  +Y  
Sbjct: 603 -LSLLVDMQKVINRLAEKGFC--DKSGHPKPNFAYFQYPQSYSVARIASILRGLANYYHL 662

Query: 602 ADNRKKVVNFCSYILRGSLAKLYAAKYKLRSRAKVYKIGARNLSRPLKEKKGQSPEYHNL 636
           A+++++ V   SYILR SLAK YAAK+KL + AKV+  G R+LS+P+K KK + P  + +
Sbjct: 663 ANSKRQCVTRLSYILRTSLAKTYAAKFKLGTAAKVFAKGGRDLSKPIKAKKARRPLLNRV 709

BLAST of MS005423 vs. ExPASy Swiss-Prot
Match: B1N1A3 (Putative nicotine oxidoreductase OS=Pseudomonas putida OX=303 GN=nicA PE=4 SV=1)

HSP 1 Score: 109.0 bits (271), Expect = 2.6e-22
Identity = 118/543 (21.73%), Postives = 211/543 (38.86%), Query Frame = 0

Query: 83  EPDRIVTNLTSYLRRFELWVLAYQKVCA--DEMGAYMPRNAIQRSALEDLLALRNAVLDN 142
           +P  +   +   + + +L++ AY+K+ +    M        +   ++  +  + N + D 
Sbjct: 20  DPSYVNDRIYRLMYKEDLYIAAYEKIKSKPGNMTPGQDGTTLDEFSIRTIRNIINKMKDE 79

Query: 143 KFKWGARLQFFIKSPKDKTDYESLSKRKIKAILTTTQAAAFQDKIVQEVLFMILEPIYEA 202
            F +    +  I     KT                   A   DK+VQEV+  ILE IYE 
Sbjct: 80  SFTFRGARRVLIPKANGKT--------------RPLSVAPPTDKVVQEVIRSILEAIYEP 139

Query: 203 RFSPKSYAFRPGRNAHTVLRVIRRHFAGYLWYVKGDLSTILDGMKVGLVINALMRDIRDK 262
            FS  S+ FR G++ HT L+ +R  ++G  W ++GD+    D +    +I+ L   I+D+
Sbjct: 140 TFSKNSHGFRAGKSCHTALKQVRESWSGVTWVIEGDIKGCFDNISHSKLIDQLRLRIKDE 199

Query: 263 KVIDLVKSALVTPVITSKVDEGEKKKKKKRKYQKKRVLAEDEPKPDPYWLETFFGFAPEE 322
           + I+L++ AL                                            G+    
Sbjct: 200 RFINLIRKALNA------------------------------------------GYFENG 259

Query: 323 AVKIPSWG--HCGILSPLLANICLDELDRWMEGRIKDFYSPSKSDVIWNSPEGEADQGNT 382
           A    + G     I+SP+LAN+ LD+LDR +E  IKD +   + D I +    +  +  T
Sbjct: 260 AFFSATLGTPQGSIISPILANVFLDQLDRKVEQLIKDHHQGEEGDKITDPAYRKLQRQKT 319

Query: 383 SWPEFVP----------------------TSGPDKTR-----KMDYIRYGGHILIGIRGP 442
           S  +                         +  P  TR     ++ Y+RY    +IG+ GP
Sbjct: 320 SLRKKAEKQEGAERDATLSLAREANSKLLSMSPYLTRNNGFIRVKYVRYADDWIIGVNGP 379

Query: 443 RADAATLRKQLIEFCDQKYMLKLDNECLPIEHI-TKGIMFLDHVLCRRVVYPTLRYTATG 502
           +  A  LR  + EF +    L+L  E   I H  ++   FL   L        +      
Sbjct: 380 KLLAEELRSVVGEFLENA-GLELSIEKTHIRHAKSETAKFLGTNLRIGSENSKIMKVLRN 439

Query: 503 GKIISEKGVGTLLSVTASLKQCIKQFRKLNFLKGDRDPDPQPCFRMFHATQAHTNAQMNK 562
           GK   ++  G    + A + + I +     F   D   +P    +           Q+  
Sbjct: 440 GKKFPKRVAGWTPVMYAPMNELIAKLHSRGFC--DPKGNPTTVNKWIFLDDNQIVEQIGS 499

Query: 563 FLLTIVEWYRYADNRKKVVNFCSYILRGSLAKLYAAKYKLRSRAKVYKIGARNLSRPLKE 594
               I+ +Y + D +  +++   +IL+ + AK  A K++  SR+K++     NL   +++
Sbjct: 500 VWRGIMNYYSFID-QFSMLSRIQFILQHAAAKTLATKHR-SSRSKIFSKHGANLRVRIRD 501

BLAST of MS005423 vs. ExPASy Swiss-Prot
Match: P0A3U0 (Group II intron-encoded protein LtrA OS=Lactococcus lactis subsp. cremoris OX=1359 GN=ltrA PE=1 SV=1)

HSP 1 Score: 106.7 bits (265), Expect = 1.3e-21
Identity = 129/526 (24.52%), Postives = 217/526 (41.25%), Query Frame = 0

Query: 85  DRIVTNLTSYLRRFELWVLAYQKVCADEMGAYMPRNAIQRSALEDLLALRNAVLDNKFKW 144
           D + T L  YL R +++ +AYQ +       Y  + A  +  L+D     +   + K K 
Sbjct: 20  DEVFTRLYRYLLRPDIYYVAYQNL-------YSNKGASTKGILDD---TADGFSEEKIK- 79

Query: 145 GARLQFFIKSPKDKTDYESLSKR-----KIKAILTTTQAAAFQDKIVQEVLFMILEPIYE 204
                  I+S KD T Y    +R     K    +       F DK++QE + +ILE IYE
Sbjct: 80  -----KIIQSLKDGTYYPQPVRRMYIAKKNSKKMRPLGIPTFTDKLIQEAVRIILESIYE 139

Query: 205 ARFSPKSYAFRPGRNAHTVLRVIRRHFAGYLWYVKGDLSTILDGMKVGLVINALMRDIRD 264
             F   S+ FRP R+ HT L+ I+R F G  W+V+GD+    D +    +I  +   I+D
Sbjct: 140 PVFEDVSHGFRPQRSCHTALKTIKREFGGARWFVEGDIKGCFDNIDHVTLIGLINLKIKD 199

Query: 265 KKVIDLVKSALVTPVITS----KVDEGEKKKKKKRKYQKKRVLAEDEPKPDPYWLETFFG 324
            K+  L+   L    + +    K   G  +            L E     D + L+    
Sbjct: 200 MKMSQLIYKFLKAGYLENWQYHKTYSGTPQGGILSPLLANIYLHE----LDKFVLQLKMK 259

Query: 325 FAPEEAVKIPSWGHCGILSPLLANICLDELDRWMEGRIKDFYSPSKSDVIWNSPEGEADQ 384
           F  E   +I         +P    +  +E+ R +  R+K      K+ V+      E  +
Sbjct: 260 FDRESPERI---------TPEYREL-HNEIKR-ISHRLKKLEGEEKAKVLL-----EYQE 319

Query: 385 GNTSWPEFVPTSGPDKTRKMDYIRYGGHILIGIRGPRADAATLRKQLIEFCDQKYMLKLD 444
                P    TS  +K  K  Y+RY    +I ++G + D   +++QL  F   K  ++L 
Sbjct: 320 KRKRLPTLPCTSQTNKVLK--YVRYADDFIISVKGSKEDCQWIKEQLKLFIHNKLKMELS 379

Query: 445 NECLPIEHITKGIMFLDH-VLCRRVVYPTLRYTATGGKIISEKGVGTLLSVTASLKQCIK 504
            E   I H ++   FL + +  RR    T++ +    K      V  L+ +   ++Q I 
Sbjct: 380 EEKTLITHSSQPARFLGYDIRVRR--SGTIKRSGKVKKRTLNGSVELLIPLQDKIRQFIF 439

Query: 505 QFRKLNFLKGDRDPDPQPCFRMFHATQAHTNAQMNKFLLTIVEWYRYADNRKKVVNFCSY 564
             +K+   K D    P     +  +T        N  L  I  +Y  A N  + +N+ +Y
Sbjct: 440 D-KKIAIQKKDSSWFPVHRKYLIRSTDLEIITIYNSELRGICNYYGLASNFNQ-LNYFAY 499

Query: 565 ILRGSLAKLYAAKYK--LRSRAKVYKIGARNLSRPLKEKKGQSPEY 599
           ++  S  K  A+K+K  L     ++K G+ +   P + K+G+   Y
Sbjct: 500 LMEYSCLKTIASKHKGTLSKTISMFKDGSGSWGIPYEIKQGKQRRY 503

BLAST of MS005423 vs. ExPASy TrEMBL
Match: A0A6J1E311 (nuclear intron maturase 2, mitochondrial OS=Momordica charantia OX=3673 GN=LOC111026013 PE=4 SV=1)

HSP 1 Score: 1466.1 bits (3794), Expect = 0.0e+00
Identity = 721/725 (99.45%), Postives = 722/725 (99.59%), Query Frame = 0

Query: 1   MHRGVSVFAHRIFASSSIVRTSNVFVFSKLNPPNSSFYGFSFCRVLSFVPPHRRTPDPDD 60
           MHRGVSVFAHRIFASSSIVRTSNVFVFSKLNPPNSSFYGFSFCRV SFVPPHRRTPDPDD
Sbjct: 1   MHRGVSVFAHRIFASSSIVRTSNVFVFSKLNPPNSSFYGFSFCRVFSFVPPHRRTPDPDD 60

Query: 61  PSTLMKEDGVSVCSQMWIENFREPDRIVTNLTSYLRRFELWVLAYQKVCADEMGAYMPRN 120
           PSTLMKEDGVSVCSQMWIENFREPDRIVTNLTSYLRRFELWVLAYQKVCADEMGAYMPRN
Sbjct: 61  PSTLMKEDGVSVCSQMWIENFREPDRIVTNLTSYLRRFELWVLAYQKVCADEMGAYMPRN 120

Query: 121 AIQRSALEDLLALRNAVLDNKFKWGARLQFFIKSPKDKTDYESLSKRKIKAILTTTQAAA 180
           AIQRSALEDLLALRNAVLDNKFKWGARLQFFIKSPKDKTDYESLSKRKIKAILTTTQAAA
Sbjct: 121 AIQRSALEDLLALRNAVLDNKFKWGARLQFFIKSPKDKTDYESLSKRKIKAILTTTQAAA 180

Query: 181 FQDKIVQEVLFMILEPIYEARFSPKSYAFRPGRNAHTVLRVIRRHFAGYLWYVKGDLSTI 240
           FQDKIVQEVLFMILEPIYEARFSPKSYAFRPGRNAHTVLRVIRRHFAGYLWYVKGDLSTI
Sbjct: 181 FQDKIVQEVLFMILEPIYEARFSPKSYAFRPGRNAHTVLRVIRRHFAGYLWYVKGDLSTI 240

Query: 241 LDGMKVGLVINALMRDIRDKKVIDLVKSALVTPVITSKVDEGEKKKKKKRKYQKKRVLAE 300
           LDGMKVGLVINALMR IRDKKVIDLVKSALVTPVITSKVDEGEKKKKKKRKYQKKRVLAE
Sbjct: 241 LDGMKVGLVINALMRGIRDKKVIDLVKSALVTPVITSKVDEGEKKKKKKRKYQKKRVLAE 300

Query: 301 DEPKPDPYWLETFFGFAPEEAVKIPSWGHCGILSPLLANICLDELDRWMEGRIKDFYSPS 360
           DEPKPDPYWLETFFGFAPEEAVKIPSWGHCGILSPLLANICLDELDRWMEGRIKDFYSPS
Sbjct: 301 DEPKPDPYWLETFFGFAPEEAVKIPSWGHCGILSPLLANICLDELDRWMEGRIKDFYSPS 360

Query: 361 KSDVIWNSPEGEADQGNTSWPEFVPTSGPDKTRKMDYIRYGGHILIGIRGPRADAATLRK 420
           KSDVIWNSPEGEADQGNTSWPEFVPTSGPDKTRKMDYIRYGGHILIGIRGPRADAATLRK
Sbjct: 361 KSDVIWNSPEGEADQGNTSWPEFVPTSGPDKTRKMDYIRYGGHILIGIRGPRADAATLRK 420

Query: 421 QLIEFCDQKYMLKLDNECLPIEHITKGIMFLDHVLCRRVVYPTLRYTATGGKIISEKGVG 480
           QLIEFCDQKYMLKLDNECLPIEHITKGIMFLDHVLCRRVVYPTLRYTATGGKIISEKGVG
Sbjct: 421 QLIEFCDQKYMLKLDNECLPIEHITKGIMFLDHVLCRRVVYPTLRYTATGGKIISEKGVG 480

Query: 481 TLLSVTASLKQCIKQFRKLNFLKGDRDPDPQPCFRMFHATQAHTNAQMNKFLLTIVEWYR 540
           TLLSVTASLKQCIKQFRKLNFLKGDRDPDPQPCFRMFHATQAHTNAQMNKFLLTIVEWYR
Sbjct: 481 TLLSVTASLKQCIKQFRKLNFLKGDRDPDPQPCFRMFHATQAHTNAQMNKFLLTIVEWYR 540

Query: 541 YADNRKKVVNFCSYILRGSLAKLYAAKYKLRSRAKVYKIGARNLSRPLKEKKGQSPEYHN 600
           YADNRKKVVNFCSYILRGSLAKLYAAKYKLRSRAKVYKIGARNLSRPLKEKKGQSPEYHN
Sbjct: 541 YADNRKKVVNFCSYILRGSLAKLYAAKYKLRSRAKVYKIGARNLSRPLKEKKGQSPEYHN 600

Query: 601 LLRMGLAESIDGLKFTRMTLVPETDYTPLPTNWRPDHEKALIEFIMLEDPRTLEEQLRCI 660
           LLRMGLAESIDGLKFTRMTLVPETDYTPLPTNWRPDHEKALIEFIMLEDPR LEEQLRCI
Sbjct: 601 LLRMGLAESIDGLKFTRMTLVPETDYTPLPTNWRPDHEKALIEFIMLEDPRMLEEQLRCI 660

Query: 661 REEGLVSAQDYISMLVWNYKRNATLDQTPLMNSGDQRLLGSSLDRLGPKAKEVEEHDDAF 720
           REEGLVSAQDYISMLVWNYKRNATLDQTPLMNSGDQRLLGSSLDRLGPKAKEVEEHDDAF
Sbjct: 661 REEGLVSAQDYISMLVWNYKRNATLDQTPLMNSGDQRLLGSSLDRLGPKAKEVEEHDDAF 720

Query: 721 RVAEL 726
           RVAE+
Sbjct: 721 RVAEV 725

BLAST of MS005423 vs. ExPASy TrEMBL
Match: A0A6J1EYF8 (nuclear intron maturase 2, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111439589 PE=4 SV=1)

HSP 1 Score: 1380.2 bits (3571), Expect = 0.0e+00
Identity = 669/725 (92.28%), Postives = 701/725 (96.69%), Query Frame = 0

Query: 1   MHRGVSVFAHRIFASSSIVRTSNVFVFSKLNPPNSSFYGFSFCRVLSFVPPHRRTPDPDD 60
           MHRGVS+FA RIF SS +V TSN F+FSKLNP + SF+GFS CRV SFVP HRRTPDPDD
Sbjct: 1   MHRGVSIFARRIFLSSRVVGTSNGFLFSKLNPLSPSFHGFSVCRVFSFVPAHRRTPDPDD 60

Query: 61  PSTLMKEDGVSVCSQMWIENFREPDRIVTNLTSYLRRFELWVLAYQKVCADEMGAYMPRN 120
           PSTLMKEDGVSVCSQMWIENFREPDRI++NLT+YLRRFELWVLAYQKVCADEMGAYMPRN
Sbjct: 61  PSTLMKEDGVSVCSQMWIENFREPDRIISNLTTYLRRFELWVLAYQKVCADEMGAYMPRN 120

Query: 121 AIQRSALEDLLALRNAVLDNKFKWGARLQFFIKSPKDKTDYESLSKRKIKAILTTTQAAA 180
           AIQRSALEDLLALRNAVLD+KFKWGARL+FFIKSPKDKTDYESLSKRKIKAILTTTQ AA
Sbjct: 121 AIQRSALEDLLALRNAVLDSKFKWGARLEFFIKSPKDKTDYESLSKRKIKAILTTTQPAA 180

Query: 181 FQDKIVQEVLFMILEPIYEARFSPKSYAFRPGRNAHTVLRVIRRHFAGYLWYVKGDLSTI 240
           FQDKIVQEVLFMILEPIYEARFSPKSYAFRPGRNAHTVLR+IRRHFAGYLWYVKGDLSTI
Sbjct: 181 FQDKIVQEVLFMILEPIYEARFSPKSYAFRPGRNAHTVLRIIRRHFAGYLWYVKGDLSTI 240

Query: 241 LDGMKVGLVINALMRDIRDKKVIDLVKSALVTPVITSKVDEGEKKKKKKRKYQKKRVLAE 300
           LDGMKVG+VINALMRDIRDKKVIDLVK+ALVTPVITSK+DEGEKKKKKKRKYQKKRVLA+
Sbjct: 241 LDGMKVGMVINALMRDIRDKKVIDLVKAALVTPVITSKIDEGEKKKKKKRKYQKKRVLAD 300

Query: 301 DEPKPDPYWLETFFGFAPEEAVKIPSWGHCGILSPLLANICLDELDRWMEGRIKDFYSPS 360
           DEPKPDPYWLETFFGFAPEEAVK PSWGHCGILSPLLANICLDELD WMEGRIKDFY+PS
Sbjct: 301 DEPKPDPYWLETFFGFAPEEAVKNPSWGHCGILSPLLANICLDELDHWMEGRIKDFYAPS 360

Query: 361 KSDVIWNSPEGEADQGNTSWPEFVPTSGPDKTRKMDYIRYGGHILIGIRGPRADAATLRK 420
           KSDVIWNSPEGEADQGNTSWPEFVPTSGPDKTRKMDY+RYGGHILIGIRGPRADAATLRK
Sbjct: 361 KSDVIWNSPEGEADQGNTSWPEFVPTSGPDKTRKMDYVRYGGHILIGIRGPRADAATLRK 420

Query: 421 QLIEFCDQKYMLKLDNECLPIEHITKGIMFLDHVLCRRVVYPTLRYTATGGKIISEKGVG 480
           QLIEFCDQKYMLKLDNECLPIEHITKGIMFLDHVLCRRVVYPTLRYTATGGKIISEKGVG
Sbjct: 421 QLIEFCDQKYMLKLDNECLPIEHITKGIMFLDHVLCRRVVYPTLRYTATGGKIISEKGVG 480

Query: 481 TLLSVTASLKQCIKQFRKLNFLKGDRDPDPQPCFRMFHATQAHTNAQMNKFLLTIVEWYR 540
           TLLSVTASLKQCIKQFRKLNFLKGDRDPDPQPCFRMFHATQAHTN+QMNKFLLTIVEWYR
Sbjct: 481 TLLSVTASLKQCIKQFRKLNFLKGDRDPDPQPCFRMFHATQAHTNSQMNKFLLTIVEWYR 540

Query: 541 YADNRKKVVNFCSYILRGSLAKLYAAKYKLRSRAKVYKIGARNLSRPLKEKKGQSPEYHN 600
           YADNR+KVVNFCSYILRGSLAKLYAAKYKLRSRAKVYKIGARNLSRPLKEKKGQSPEYHN
Sbjct: 541 YADNRRKVVNFCSYILRGSLAKLYAAKYKLRSRAKVYKIGARNLSRPLKEKKGQSPEYHN 600

Query: 601 LLRMGLAESIDGLKFTRMTLVPETDYTPLPTNWRPDHEKALIEFIMLEDPRTLEEQLRCI 660
           LLRMGLAESIDGLKFTRM+LVPETDYTPLP NWRPDHEKAL+EFIMLEDPRTLEE  RCI
Sbjct: 601 LLRMGLAESIDGLKFTRMSLVPETDYTPLPNNWRPDHEKALLEFIMLEDPRTLEEHRRCI 660

Query: 661 REEGLVSAQDYISMLVWNYKRNATLDQTPLMNSGDQRLLGSSLDRLGPKAKEVEEHDDAF 720
           RE+GLVS QDYISMLVWNYKRNA++DQT LMN+ D R+LGS+L  LG KAKE+EEHD++F
Sbjct: 661 REQGLVSPQDYISMLVWNYKRNASMDQTSLMNTIDHRILGSNLGSLGSKAKELEEHDESF 720

Query: 721 RVAEL 726
           + AE+
Sbjct: 721 QAAEV 725

BLAST of MS005423 vs. ExPASy TrEMBL
Match: A0A6J1I719 (nuclear intron maturase 2, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111471814 PE=4 SV=1)

HSP 1 Score: 1374.8 bits (3557), Expect = 0.0e+00
Identity = 667/725 (92.00%), Postives = 697/725 (96.14%), Query Frame = 0

Query: 1   MHRGVSVFAHRIFASSSIVRTSNVFVFSKLNPPNSSFYGFSFCRVLSFVPPHRRTPDPDD 60
           MHRGVS+FA RIF SS +V TSN  +FSKLNP N SF+GFS CRV SFVP HRRTP PDD
Sbjct: 1   MHRGVSIFARRIFLSSRVVGTSNGLLFSKLNPLNPSFHGFSVCRVFSFVPAHRRTPGPDD 60

Query: 61  PSTLMKEDGVSVCSQMWIENFREPDRIVTNLTSYLRRFELWVLAYQKVCADEMGAYMPRN 120
           PSTLMKEDGVSVCSQMWIENFREPDRI++NLT+YLRRFELWVLAYQKVCAD+MGAYMPRN
Sbjct: 61  PSTLMKEDGVSVCSQMWIENFREPDRIISNLTTYLRRFELWVLAYQKVCADDMGAYMPRN 120

Query: 121 AIQRSALEDLLALRNAVLDNKFKWGARLQFFIKSPKDKTDYESLSKRKIKAILTTTQAAA 180
           AIQRSALEDLLALRNAVLD+KFKWGARL+FFIKSPKDKTDYESLSKRKIKAILTTTQ AA
Sbjct: 121 AIQRSALEDLLALRNAVLDSKFKWGARLEFFIKSPKDKTDYESLSKRKIKAILTTTQPAA 180

Query: 181 FQDKIVQEVLFMILEPIYEARFSPKSYAFRPGRNAHTVLRVIRRHFAGYLWYVKGDLSTI 240
           FQDKIVQEVLFMILEPIYEARFSPKSYAFRPGRNAHTVLRVIRRHFAGYLWYVKGDLSTI
Sbjct: 181 FQDKIVQEVLFMILEPIYEARFSPKSYAFRPGRNAHTVLRVIRRHFAGYLWYVKGDLSTI 240

Query: 241 LDGMKVGLVINALMRDIRDKKVIDLVKSALVTPVITSKVDEGEKKKKKKRKYQKKRVLAE 300
           LDGMKVG+VINALMRDIRDKKVIDLVK+ALVTPVITS +DEGEKKKKKKRKYQKKRVLA+
Sbjct: 241 LDGMKVGMVINALMRDIRDKKVIDLVKAALVTPVITSNIDEGEKKKKKKRKYQKKRVLAD 300

Query: 301 DEPKPDPYWLETFFGFAPEEAVKIPSWGHCGILSPLLANICLDELDRWMEGRIKDFYSPS 360
           DEPKPDPYWLETFFGFAPEEAVK PSWGHCGILSPLLANICLDELD WMEGRIKDFYSPS
Sbjct: 301 DEPKPDPYWLETFFGFAPEEAVKNPSWGHCGILSPLLANICLDELDHWMEGRIKDFYSPS 360

Query: 361 KSDVIWNSPEGEADQGNTSWPEFVPTSGPDKTRKMDYIRYGGHILIGIRGPRADAATLRK 420
           KSDVIWNSPEGEADQGNTSWPEFVPTSGPDKTRKMDY+RYGGHILIGIRGPRADAATLRK
Sbjct: 361 KSDVIWNSPEGEADQGNTSWPEFVPTSGPDKTRKMDYVRYGGHILIGIRGPRADAATLRK 420

Query: 421 QLIEFCDQKYMLKLDNECLPIEHITKGIMFLDHVLCRRVVYPTLRYTATGGKIISEKGVG 480
           QLIEFCDQKYMLKLDNECLPIEHITKGIMFLDHVLCRRVVYPTLRYTATGGKIISEKGVG
Sbjct: 421 QLIEFCDQKYMLKLDNECLPIEHITKGIMFLDHVLCRRVVYPTLRYTATGGKIISEKGVG 480

Query: 481 TLLSVTASLKQCIKQFRKLNFLKGDRDPDPQPCFRMFHATQAHTNAQMNKFLLTIVEWYR 540
           TLLSVTASLKQCIKQFR+LNFLKGDRDPDPQPCFRMFHATQAHTN+QMNKFLLTIVEWYR
Sbjct: 481 TLLSVTASLKQCIKQFRRLNFLKGDRDPDPQPCFRMFHATQAHTNSQMNKFLLTIVEWYR 540

Query: 541 YADNRKKVVNFCSYILRGSLAKLYAAKYKLRSRAKVYKIGARNLSRPLKEKKGQSPEYHN 600
           YADNR+KVVNFCSYILRGSLAKLYAAKYKLRSRAKVYKIGARNLSRPLKEKKGQSPEYHN
Sbjct: 541 YADNRRKVVNFCSYILRGSLAKLYAAKYKLRSRAKVYKIGARNLSRPLKEKKGQSPEYHN 600

Query: 601 LLRMGLAESIDGLKFTRMTLVPETDYTPLPTNWRPDHEKALIEFIMLEDPRTLEEQLRCI 660
           LLRMGLAESIDGLKFTRM+LVPETDYTPLP NWRPDHEKAL+EFIMLEDPRTLEE  RCI
Sbjct: 601 LLRMGLAESIDGLKFTRMSLVPETDYTPLPNNWRPDHEKALLEFIMLEDPRTLEEHRRCI 660

Query: 661 REEGLVSAQDYISMLVWNYKRNATLDQTPLMNSGDQRLLGSSLDRLGPKAKEVEEHDDAF 720
            E+GLVS QDYISMLVWNYKRNAT+DQT LMN+ D R+LGS+LD  G KAKE+EEHD++F
Sbjct: 661 SEQGLVSPQDYISMLVWNYKRNATMDQTSLMNTIDHRILGSNLDSFGSKAKELEEHDESF 720

Query: 721 RVAEL 726
           + AE+
Sbjct: 721 QAAEV 725

BLAST of MS005423 vs. ExPASy TrEMBL
Match: A0A0A0LF81 (Intron_maturas2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G910700 PE=4 SV=1)

HSP 1 Score: 1352.0 bits (3498), Expect = 0.0e+00
Identity = 653/722 (90.44%), Postives = 691/722 (95.71%), Query Frame = 0

Query: 1   MHRGVSVFAHRIFASSSIVRTSNVFVFSKLNPPNSSFYGFSFCRVLSFVPPHRRTPDPDD 60
           MHRGV +FAHRIFASSS+VRTS+ F+FS+LNP  SSF+GF  CRV SFVP HRR PDP+D
Sbjct: 1   MHRGVFIFAHRIFASSSVVRTSSGFIFSELNPLKSSFHGFPLCRVFSFVPAHRRAPDPND 60

Query: 61  PSTLMKEDGVSVCSQMWIENFREPDRIVTNLTSYLRRFELWVLAYQKVCADEMGAYMPRN 120
           PS LMKEDG+S CSQMWIENFREPDRIV+NLT+YL++FELWVLAYQKVCADEMG+YMPRN
Sbjct: 61  PSNLMKEDGISACSQMWIENFREPDRIVSNLTTYLQKFELWVLAYQKVCADEMGSYMPRN 120

Query: 121 AIQRSALEDLLALRNAVLDNKFKWGARLQFFIKSPKDKTDYESLSKRKIKAILTTTQAAA 180
           AIQRSALEDLLALRNAVLD++F WGARL+FFIKSPKDKTDYE+LSKRKIKAILTTTQ AA
Sbjct: 121 AIQRSALEDLLALRNAVLDSRFNWGARLKFFIKSPKDKTDYEALSKRKIKAILTTTQPAA 180

Query: 181 FQDKIVQEVLFMILEPIYEARFSPKSYAFRPGRNAHTVLRVIRRHFAGYLWYVKGDLSTI 240
           FQDKIVQEVLF+ILEPIYEARFSPKSYAFRPGRNAHTVLRVIRRHFAGYLWYVKGDLSTI
Sbjct: 181 FQDKIVQEVLFLILEPIYEARFSPKSYAFRPGRNAHTVLRVIRRHFAGYLWYVKGDLSTI 240

Query: 241 LDGMKVGLVINALMRDIRDKKVIDLVKSALVTPVITSKVDEGEKKKKKKRKYQKKRVLAE 300
           LDGMKVG VINALMRDIRDKKVIDL+KSALVTPVITSK+DEGEKKKKKKRKYQKK+VLAE
Sbjct: 241 LDGMKVGAVINALMRDIRDKKVIDLIKSALVTPVITSKIDEGEKKKKKKRKYQKKKVLAE 300

Query: 301 DEPKPDPYWLETFFGFAPEEAVKIPSWGHCGILSPLLANICLDELDRWMEGRIKDFYSPS 360
           DEPKPDPYWLETFFGFAPEEAVK PSWGHCGILSPLLANICLDELD WMEG+IKDFYSPS
Sbjct: 301 DEPKPDPYWLETFFGFAPEEAVKNPSWGHCGILSPLLANICLDELDHWMEGKIKDFYSPS 360

Query: 361 KSDVIWNSPEGEADQGNTSWPEFVPTSGPDKTRKMDYIRYGGHILIGIRGPRADAATLRK 420
           KSDVIWNSPEGEADQGNTSWPEFVPTSGPDKTRKMDYIRYGGHILIG+RGPRADAATLRK
Sbjct: 361 KSDVIWNSPEGEADQGNTSWPEFVPTSGPDKTRKMDYIRYGGHILIGVRGPRADAATLRK 420

Query: 421 QLIEFCDQKYMLKLDNECLPIEHITKGIMFLDHVLCRRVVYPTLRYTATGGKIISEKGVG 480
           QLIEFCD+KYMLKLD+ECLPIEHITKGIMFLDHVLCRRVVYPTLRYTA+GGKIISEKGVG
Sbjct: 421 QLIEFCDEKYMLKLDSECLPIEHITKGIMFLDHVLCRRVVYPTLRYTASGGKIISEKGVG 480

Query: 481 TLLSVTASLKQCIKQFRKLNFLKGDRDPDPQPCFRMFHATQAHTNAQMNKFLLTIVEWYR 540
           TLLSVTASLKQCIKQFRKL+F+KGDRDPDPQPCFRMFHATQAHTN+QMNKFLLTIVEWY+
Sbjct: 481 TLLSVTASLKQCIKQFRKLSFIKGDRDPDPQPCFRMFHATQAHTNSQMNKFLLTIVEWYK 540

Query: 541 YADNRKKVVNFCSYILRGSLAKLYAAKYKLRSRAKVYKIGARNLSRPLKEKKGQSPEYHN 600
           YADNR+KVVNFCSYILRGSLAKLYAAKYKLRSRAKVYKIGARNLSRPLKEKKGQSPEYHN
Sbjct: 541 YADNRRKVVNFCSYILRGSLAKLYAAKYKLRSRAKVYKIGARNLSRPLKEKKGQSPEYHN 600

Query: 601 LLRMGLAESIDGLKFTRMTLVPETDYTPLPTNWRPDHEKALIEFIMLEDPRTLEEQLRCI 660
           LLRMGLAESIDGLKFTRM+LVPETDYTPLP NWRPDHEKAL+EFIMLEDPRTLEEQ RCI
Sbjct: 601 LLRMGLAESIDGLKFTRMSLVPETDYTPLPNNWRPDHEKALLEFIMLEDPRTLEEQRRCI 660

Query: 661 REEGLVSAQDYISMLVWNYKRNATLDQTPLMNSGDQRLLGSSLDRLGPKAKEVEEHDDAF 720
           RE GLVS QDYISMLVWNYKRNAT+DQ  LMNSGD R+LG +L   G K+KE+EEHD A 
Sbjct: 661 RELGLVSPQDYISMLVWNYKRNATMDQMSLMNSGDHRILGLNLGSHGSKSKELEEHDQAA 720

Query: 721 RV 723
            V
Sbjct: 721 EV 722

BLAST of MS005423 vs. ExPASy TrEMBL
Match: A0A5D3E763 (Putative mitochondrial protein ymf11 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold455G004040 PE=4 SV=1)

HSP 1 Score: 1351.7 bits (3497), Expect = 0.0e+00
Identity = 658/722 (91.14%), Postives = 690/722 (95.57%), Query Frame = 0

Query: 1   MHRGVSVFAHRIFASSSIVRTSNVFVFSKLNPPNSSFYGFSFCRVLSFVPPHRRTPDPDD 60
           MHRGVS+FAHRIFASSS+VRTS+ F+FS+LNP  SSF+GF  CRV SF+P HRR PDPDD
Sbjct: 1   MHRGVSIFAHRIFASSSVVRTSSGFLFSELNPLKSSFHGFPLCRVFSFMPAHRRAPDPDD 60

Query: 61  PSTLMKEDGVSVCSQMWIENFREPDRIVTNLTSYLRRFELWVLAYQKVCADEMGAYMPRN 120
           PS L+KEDG+SVCSQMWIENFREPDRIV+NLT+YLRRFELWVLAYQKVC DEMGAYMPRN
Sbjct: 61  PSNLLKEDGISVCSQMWIENFREPDRIVSNLTTYLRRFELWVLAYQKVCTDEMGAYMPRN 120

Query: 121 AIQRSALEDLLALRNAVLDNKFKWGARLQFFIKSPKDKTDYESLSKRKIKAILTTTQAAA 180
           AIQRSALEDLLALRNAVLD++FKWGARL+FFIKSPKDKTDY +LSKRKIKAILTTTQ AA
Sbjct: 121 AIQRSALEDLLALRNAVLDSRFKWGARLEFFIKSPKDKTDYGALSKRKIKAILTTTQPAA 180

Query: 181 FQDKIVQEVLFMILEPIYEARFSPKSYAFRPGRNAHTVLRVIRRHFAGYLWYVKGDLSTI 240
           FQDKIVQEVLFMILEPIYEARFS KSYAFRPGRNAHTVLRVIRRHFAGYLWYVKGDLSTI
Sbjct: 181 FQDKIVQEVLFMILEPIYEARFSSKSYAFRPGRNAHTVLRVIRRHFAGYLWYVKGDLSTI 240

Query: 241 LDGMKVGLVINALMRDIRDKKVIDLVKSALVTPVITSKVDEGEKKKKKKRKYQKKRVLAE 300
           LDGMKVG VINALMRDIRDKKVIDL+KSALVTPVITSK+DEGE KKKKKRKYQKKRVLA+
Sbjct: 241 LDGMKVGAVINALMRDIRDKKVIDLIKSALVTPVITSKIDEGE-KKKKKRKYQKKRVLAD 300

Query: 301 DEPKPDPYWLETFFGFAPEEAVKIPSWGHCGILSPLLANICLDELDRWMEGRIKDFYSPS 360
           DEPKPDPYWLETFFGFAPEEAVK PSWGHCGILSPLLANICLDELDRWMEG+IKDFYSPS
Sbjct: 301 DEPKPDPYWLETFFGFAPEEAVKNPSWGHCGILSPLLANICLDELDRWMEGKIKDFYSPS 360

Query: 361 KSDVIWNSPEGEADQGNTSWPEFVPTSGPDKTRKMDYIRYGGHILIGIRGPRADAATLRK 420
           KSDVIWNSPEGEADQGNTSWPEFVPTSGPDKTRKMDYIRYGGHILIGIRGPRADAATLRK
Sbjct: 361 KSDVIWNSPEGEADQGNTSWPEFVPTSGPDKTRKMDYIRYGGHILIGIRGPRADAATLRK 420

Query: 421 QLIEFCDQKYMLKLDNECLPIEHITKGIMFLDHVLCRRVVYPTLRYTATGGKIISEKGVG 480
           QLIEFCD+KYMLKLD+ECLPIEHITKGIMFLDHVLCRRVVYPTLRYTA+GGKIISEKGVG
Sbjct: 421 QLIEFCDEKYMLKLDSECLPIEHITKGIMFLDHVLCRRVVYPTLRYTASGGKIISEKGVG 480

Query: 481 TLLSVTASLKQCIKQFRKLNFLKGDRDPDPQPCFRMFHATQAHTNAQMNKFLLTIVEWYR 540
           TLLSVTASLKQCIKQFRKLNFLKGDRDPDPQPCFRMFHATQAHTN+QMNKFLLTIVEWY+
Sbjct: 481 TLLSVTASLKQCIKQFRKLNFLKGDRDPDPQPCFRMFHATQAHTNSQMNKFLLTIVEWYK 540

Query: 541 YADNRKKVVNFCSYILRGSLAKLYAAKYKLRSRAKVYKIGARNLSRPLKEKKGQSPEYHN 600
           YADNR+KVVNFCSYILRGSLAKLYAAKYKLRSRAKVYKIGARNLSRPLKEKKGQSPEYHN
Sbjct: 541 YADNRRKVVNFCSYILRGSLAKLYAAKYKLRSRAKVYKIGARNLSRPLKEKKGQSPEYHN 600

Query: 601 LLRMGLAESIDGLKFTRMTLVPETDYTPLPTNWRPDHEKALIEFIMLEDPRTLEEQLRCI 660
           LLRMGLAESIDGLKFTRM+LVPETDYTPLP NWRPDHEKAL+EFIMLEDPRTLEEQ  CI
Sbjct: 601 LLRMGLAESIDGLKFTRMSLVPETDYTPLPNNWRPDHEKALLEFIMLEDPRTLEEQRSCI 660

Query: 661 REEGLVSAQDYISMLVWNYKRNATLDQTPLMNSGDQRLLGSSLDRLGPKAKEVEEHDDAF 720
           RE GLVS QDYISMLVWNYKRNAT+DQ  LMNSGD R+LG +LD    K+KE+EEHD A 
Sbjct: 661 RELGLVSPQDYISMLVWNYKRNATMDQMSLMNSGDHRILGLNLDSHDSKSKELEEHDQAA 720

Query: 721 RV 723
            V
Sbjct: 721 EV 721

BLAST of MS005423 vs. TAIR 10
Match: AT5G46920.1 (Intron maturase, type II family protein )

HSP 1 Score: 1141.7 bits (2952), Expect = 0.0e+00
Identity = 567/738 (76.83%), Postives = 634/738 (85.91%), Query Frame = 0

Query: 1   MHRGVSVFAHRIFASSSIVRTSNVFVFSKLNPP--NSSFYGFSFCRVLSFVPPHRR---- 60
           M R  SV     +   S   T NV+ F   NPP  ++   G +  R LS+  P +R    
Sbjct: 1   MRRSFSVLGPYKWLKPSSTYT-NVYCF-VTNPPLISNPNNGSAIFRYLSYFAPQQRRQQQ 60

Query: 61  -TPDPDDPSTLMKEDGVSVCSQMWIENFREPDRIVTNLTSYLRRFELWVLAYQKVCADEM 120
             PDPDDP+ L+KEDGVS+CSQMW+ENF+EPD+  TNLTSYLRRFELWVLAYQKVC DE+
Sbjct: 61  QAPDPDDPANLLKEDGVSLCSQMWLENFKEPDKTATNLTSYLRRFELWVLAYQKVCCDEL 120

Query: 121 GAYMPRNAIQRSALEDLLALRNAVLDNKFKWGARLQFFIKSPKDKTDYESLSKRKIKAIL 180
           GAY+PR++IQRSALE+LLALRN+VLD++FKWG+RL F+IKSP+DKTDYESLSKRKIKAIL
Sbjct: 121 GAYVPRSSIQRSALENLLALRNSVLDDRFKWGSRLDFYIKSPRDKTDYESLSKRKIKAIL 180

Query: 181 TTTQAAAFQDKIVQEVLFMILEPIYEARFSPKSYAFRPGRNAHTVLRVIRRHFAGYLWYV 240
           TTTQ   FQD+IVQEVL MILEPIYE+RFS KS+AFRPGR AHTVLRVIRR+FAGYLWYV
Sbjct: 181 TTTQPTPFQDRIVQEVLLMILEPIYESRFSQKSFAFRPGRTAHTVLRVIRRNFAGYLWYV 240

Query: 241 KGDLSTILDGMKVGLVINALMRDIRDKKVIDLVKSALVTPVITSKVDEGEKKKKKKRKYQ 300
           KGDLS +LDGMKVG VI++LMRD+RDKKVIDL+KSALVTPV+TSKV++GEKKK KKRKYQ
Sbjct: 241 KGDLSVVLDGMKVGFVISSLMRDVRDKKVIDLIKSALVTPVVTSKVEDGEKKKTKKRKYQ 300

Query: 301 KKRVLAEDEPKPDPYWLETFFGFAPEEAVKIPSWGHCGILSPLLANICLDELDRWMEGRI 360
           KKRVLAEDEPKPDPYWLETFFGFAPEEA K P WGHCGILSPLL N+CLDELDRWME ++
Sbjct: 301 KKRVLAEDEPKPDPYWLETFFGFAPEEAGKSPQWGHCGILSPLLVNVCLDELDRWMETKV 360

Query: 361 KDFYSPSKSDVIWNSPEGEADQGNTSWPEFVPTSGPDKTRKMDYIRYGGHILIGIRGPRA 420
           KDFY PSKSDVIWN+PEGEADQGNTSWPEFVPTSGPDKTRKMDY+RYGGHILIG+RGPRA
Sbjct: 361 KDFYRPSKSDVIWNNPEGEADQGNTSWPEFVPTSGPDKTRKMDYVRYGGHILIGVRGPRA 420

Query: 421 DAATLRKQLIEFCDQKYMLKLDNECLPIEHITKGIMFLDHVLCRRVVYPTLRYTATGGKI 480
           DAATLRK+LIEF DQKYML+LDNE LPIEHITKGIMFLDHVLCRRVVYPTLRYTATGGKI
Sbjct: 421 DAATLRKELIEFVDQKYMLRLDNENLPIEHITKGIMFLDHVLCRRVVYPTLRYTATGGKI 480

Query: 481 ISEKGVGTLLSVTASLKQCIKQFRKLNFLKGDRDPDPQPCFRMFHATQAHTNAQMNKFLL 540
           ISEKGVGTLLSVTASLKQCIKQFRKL F+KGDRDPDPQPCFRMFHATQAHTN QMNKFL 
Sbjct: 481 ISEKGVGTLLSVTASLKQCIKQFRKLLFIKGDRDPDPQPCFRMFHATQAHTNNQMNKFLT 540

Query: 541 TIVEWYRYADNRKKVVNFCSYILRGSLAKLYAAKYKLRSRAKVYKIGARNLSRPLKEKKG 600
           TI EWYR+ADNRKK+VNFCSYI+RGSLAKLYAAKYKLRSRAKVYK   RNLS PL +KKG
Sbjct: 541 TIAEWYRFADNRKKIVNFCSYIIRGSLAKLYAAKYKLRSRAKVYKFANRNLSLPLLQKKG 600

Query: 601 QSPEYHNLLRMGLAESIDGLKFTRMTLVPETDYTPLPTNWRPDHEKALIEFIMLEDPRTL 660
           QSPEY NLLRMGLAES+DGL +TRM+LVPETDY+P P NWRP+HEK LIE++ L++P+TL
Sbjct: 601 QSPEYQNLLRMGLAESVDGLVYTRMSLVPETDYSPFPGNWRPEHEKFLIEYLTLDEPKTL 660

Query: 661 EEQLRCIREEGLVSAQDYISMLVWNYKRNA-TLDQTPLMNSGDQRLLGSS----LDRLGP 720
           EEQ R IRE+GLVS QDY SMLVWNYKRNA  +DQ  ++      LLGSS     D    
Sbjct: 661 EEQKRFIREKGLVSPQDYTSMLVWNYKRNAIPMDQVSILKD-QPFLLGSSSTYNRDNDDQ 720

Query: 721 KAKEV-EEHDDAFRVAEL 726
           K KE  E+ +D  R+A +
Sbjct: 721 KNKEEDEDSEDGLRIARM 735

BLAST of MS005423 vs. TAIR 10
Match: AT1G30010.1 (Intron maturase, type II family protein )

HSP 1 Score: 729.6 bits (1882), Expect = 2.9e-210
Identity = 348/647 (53.79%), Postives = 482/647 (74.50%), Query Frame = 0

Query: 53  RRTPDPDDPSTLMKEDGVSVCSQMWIENFRE-PDRIVTNLTSYLRRFELWVLAYQKVCAD 112
           R      DP +L+K+D V +C  +W+++F   P    +NLT +L +F+LWVLAYQ+ CA 
Sbjct: 41  REPSSTQDPYSLLKQDPVDICLSLWVKSFSSPPSATFSNLTGFLSKFDLWVLAYQRTCAH 100

Query: 113 EMGAYMPRNAIQRSALEDLLALRNAVL--DNKFKWGARLQFFIKSPKDKTDY---ESLSK 172
             G + PRNAI  +AL  LL+L+NAV     KF+W  ++  +++SPKDK      E +SK
Sbjct: 101 VTGTFPPRNAIHANALRSLLSLQNAVTRSGGKFRWNDKMNQYVRSPKDKISMNGGEGMSK 160

Query: 173 RKIKAILTTTQAAAFQDKIVQEVLFMILEPIYEARFSPKSYAFRPGRNAHTVLRVIRRHF 232
            K++ I+  ++   FQD++V EVL MILEP +EARFS KS+ FRPGRN HTV+R IR +F
Sbjct: 161 GKVRRII-ESEEPIFQDRVVHEVLLMILEPFFEARFSSKSHGFRPGRNPHTVIRTIRSNF 220

Query: 233 AGYLWYVKGDLSTILDGMKVGLVINALMRDIRDKKVIDLVKSALV---TPVITSKVDE-- 292
           AGYLW++KGD+S +LD + V +V+N L + ++D+KV+ L++S+L      V+   V++  
Sbjct: 221 AGYLWFMKGDVSEMLDHVDVDVVMNCLQKVVKDRKVLGLIESSLKFSDKRVLKRVVEKHG 280

Query: 293 -----GEKKK--KKKRKYQKKRVLAEDEPKPDPYWLETFFGFAPEEAVKIPSWGHCGILS 352
                G K++  ++KR   KK++L++DEPKPDPYWL TF+ FAP+EA K+PS+G+CG+LS
Sbjct: 281 NDNGLGTKRRIEREKRNKTKKKILSDDEPKPDPYWLRTFYSFAPKEAAKVPSYGYCGVLS 340

Query: 353 PLLANICLDELDRWMEGRIKDFYSPSKSDVIWNSPEGEADQGNTSWPEFVPTSGPDKTRK 412
           PLLAN+CL+ELDR+ME +I +++SP K D IW     E    N +WPEFVP+SG +KTRK
Sbjct: 341 PLLANVCLNELDRFMETKIVEYFSPCKDDSIWKE-SIEDGCHNPAWPEFVPSSGKEKTRK 400

Query: 413 MDYIRYGGHILIGIRGPRADAATLRKQLIEFCDQKYMLKLDNECLPIEHITKGIMFLDHV 472
           MDYIRYGGH LIGIRGPR DA  +RK++I+FCD+ + ++LDN  L IEHI++GI FLDH+
Sbjct: 401 MDYIRYGGHFLIGIRGPREDAVKMRKEIIDFCDRVFGVRLDNSKLEIEHISRGIQFLDHI 460

Query: 473 LCRRVVYPTLRYTATGGKIISEKGVGTLLSVTASLKQCIKQFRKLNFLKGDRDPDPQPCF 532
           +CRRV+YPTLRYT +GG I+S+KGVGTLLSV+ASL+QCI+QFR+L F+KGD+DP+P PC 
Sbjct: 461 ICRRVIYPTLRYTGSGGSIVSKKGVGTLLSVSASLEQCIRQFRRLAFVKGDKDPEPLPCN 520

Query: 533 RMFHATQAHTNAQMNKFLLTIVEWYRYADNRKKVVNFCSYILRGSLAKLYAAKYKLRSRA 592
            M +++Q+H+N+QMNKFL T+ +WY+YADNRKK V FC+Y++R SLAKLYAA+Y+L+SRA
Sbjct: 521 PMLYSSQSHSNSQMNKFLETMADWYKYADNRKKAVGFCAYVIRSSLAKLYAARYRLKSRA 580

Query: 593 KVYKIGARNLSRPLKEKKGQS-PEYHNLLRMGLAESIDGLKFTRMTLVPETDYTPLPTNW 652
           KVY I +R+LS PL E    S PEY +LLRMGL ++I+G++F+RM+L+P  DYTP P NW
Sbjct: 581 KVYSIASRDLSHPLSESSNNSAPEYSDLLRMGLVDAIEGVQFSRMSLIPSCDYTPFPRNW 640

Query: 653 RPDHEKALIEFIMLEDPRTLEEQLRCIREEGLVSAQDYISMLVWNYK 681
            P+HE+ L E+I L+DP+      R I+ EGL   QD IS  VW++K
Sbjct: 641 IPNHEQVLQEYIRLQDPKFFCGLHRSIKREGLTLPQDEISEAVWDFK 685

BLAST of MS005423 vs. TAIR 10
Match: AT5G04050.1 (RNA-directed DNA polymerase (reverse transcriptase) )

HSP 1 Score: 48.1 bits (113), Expect = 3.8e-05
Identity = 54/253 (21.34%), Postives = 104/253 (41.11%), Query Frame = 0

Query: 184 KIVQEVLFMILEPIYEARFSPKSYAFRPGRNAHTVLRVIRRHFAGYLWYVKGDLS-TILD 243
           K++ E + M+LE +Y+ RF+  SY  R G   HT +R ++       W+ +   +  + +
Sbjct: 137 KVLIEAIRMVLEIVYDDRFATFSYGGRVGMGRHTAIRYLKNSVENPRWWFRVSFAREMFE 196

Query: 244 GMKVGLVINALMRDIRDKKVIDLVKSALVTPVITSKVDEGEKKKKKKRKYQKKRVLAEDE 303
              V ++   +   I D  +I+++K      ++  K++ G     +              
Sbjct: 197 ERNVDILCGFVGEKINDVMLIEMIKKLFEFGIL--KIELGGCNSGR-------------- 256

Query: 304 PKPDPYWLETFFGFAPEEAVKIPSWGHCGILSPLLANICLDELDRWMEGRIKDFYSPSKS 363
                       GF P+E         CG+ S +L N+  D LD+     I+D     K 
Sbjct: 257 ------------GF-PQE---------CGLCS-ILINVYFDGLDK----EIQDLRLKMK- 316

Query: 364 DVIWNSPEGEADQGNTSWPEFVPTSGPDKTRKMDYIRYGGHILIGIRGPRADAATLRKQL 423
             + N   G  D+ +T    F P +       +  +RY   IL+   G +     L+K++
Sbjct: 317 --VKNPRVGTGDEESTGNVFFKPVN-------IYAVRYLDEILVITSGSKMLTMDLKKRI 336

Query: 424 IEFCDQKYMLKLD 436
           ++  +Q+  L++D
Sbjct: 377 VDILEQRLELRVD 336

BLAST of MS005423 vs. TAIR 10
Match: AT5G04050.2 (RNA-directed DNA polymerase (reverse transcriptase) )

HSP 1 Score: 48.1 bits (113), Expect = 3.8e-05
Identity = 54/253 (21.34%), Postives = 104/253 (41.11%), Query Frame = 0

Query: 184 KIVQEVLFMILEPIYEARFSPKSYAFRPGRNAHTVLRVIRRHFAGYLWYVKGDLS-TILD 243
           K++ E + M+LE +Y+ RF+  SY  R G   HT +R ++       W+ +   +  + +
Sbjct: 137 KVLIEAIRMVLEIVYDDRFATFSYGGRVGMGRHTAIRYLKNSVENPRWWFRVSFAREMFE 196

Query: 244 GMKVGLVINALMRDIRDKKVIDLVKSALVTPVITSKVDEGEKKKKKKRKYQKKRVLAEDE 303
              V ++   +   I D  +I+++K      ++  K++ G     +              
Sbjct: 197 ERNVDILCGFVGEKINDVMLIEMIKKLFEFGIL--KIELGGCNSGR-------------- 256

Query: 304 PKPDPYWLETFFGFAPEEAVKIPSWGHCGILSPLLANICLDELDRWMEGRIKDFYSPSKS 363
                       GF P+E         CG+ S +L N+  D LD+     I+D     K 
Sbjct: 257 ------------GF-PQE---------CGLCS-ILINVYFDGLDK----EIQDLRLKMK- 316

Query: 364 DVIWNSPEGEADQGNTSWPEFVPTSGPDKTRKMDYIRYGGHILIGIRGPRADAATLRKQL 423
             + N   G  D+ +T    F P +       +  +RY   IL+   G +     L+K++
Sbjct: 317 --VKNPRVGTGDEESTGNVFFKPVN-------IYAVRYLDEILVITSGSKMLTMDLKKRI 336

Query: 424 IEFCDQKYMLKLD 436
           ++  +Q+  L++D
Sbjct: 377 VDILEQRLELRVD 336

BLAST of MS005423 vs. TAIR 10
Match: ATMG00520.1 (Intron maturase, type II family protein )

HSP 1 Score: 45.8 bits (107), Expect = 1.9e-04
Identity = 25/79 (31.65%), Postives = 43/79 (54.43%), Query Frame = 0

Query: 183 DKIVQEVLFMILEPIYEARFSPKSYAFRPGRNAHTVLRVIRRHFAGYLWYVKGDLSTILD 242
           +KI++E + M+LE IY+  F P +  FR G+  H+VLR I+  +    W+++ D+     
Sbjct: 14  EKIMKEAIRMVLESIYDPEF-PDTSHFRSGQGCHSVLRRIKEEWGISRWFLEFDIRKCFH 73

Query: 243 GMKVGLVINALMRDIRDKK 262
            +    +I  L  +I D K
Sbjct: 74  TIDRHRLIQILKEEIDDPK 91