MS005422 (gene) Bitter gourd (TR) v1

Overview


Name	MS005422
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Plant invertase/pectin methylesterase inhibitor superfamily protein
Location	scaffold641: 85948 .. 86514 (+)
RNA-Seq Expression	MS005422
Synteny	MS005422

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

AAGATGGAGAAGAAGCTCTTTCTTCTAATTCTACTGCTTTTCTCTCTCCTCTCACTTTGCCAATCGGAATCGGCGCCCGCAAACGACGTCGTTCCACGCTCCTGCAAGCAGGCCGCGACGCGAGACCCATACGTGGACTACAAACTCTGCGTCGAGGCCCTCAGGGCCAATCCCAAGGCCCGAAACGGTGCGTTTAAGGACCTGGTCCTGATCTCCATCGACCAAGCTAAGGCCAACGCCACGGAAATCGGGTCCGAGATTTCAGAGATTTTGAAGGGCAGCGGCAAATGGGGGACGAAGCCGAAGACCAAGACCAAGTATTCGTTAGATTGCTTGAGGAGCTGTTTGGAGCTTTATTCTGAGGCTGTTTCTGATCTGAAATCGGCTGTTTCTGCAGTGAAAATGGAGGATTACGAGACGGCGAAAGTGGAGGTGAGCGCCGCCATTGATGCACCTGTTAGTTGTGAAGATGGGTATAAGGAGAAGGATGGGGAGGTTTCTCCATTGACGGAGAAGAATGATGTGTTCTTCCATTTGGCTGCCATTTCTCTTGCGTTTGTTGATATG

mRNA sequence

AAGATGGAGAAGAAGCTCTTTCTTCTAATTCTACTGCTTTTCTCTCTCCTCTCACTTTGCCAATCGGAATCGGCGCCCGCAAACGACGTCGTTCCACGCTCCTGCAAGCAGGCCGCGACGCGAGACCCATACGTGGACTACAAACTCTGCGTCGAGGCCCTCAGGGCCAATCCCAAGGCCCGAAACGGTGCGTTTAAGGACCTGGTCCTGATCTCCATCGACCAAGCTAAGGCCAACGCCACGGAAATCGGGTCCGAGATTTCAGAGATTTTGAAGGGCAGCGGCAAATGGGGGACGAAGCCGAAGACCAAGACCAAGTATTCGTTAGATTGCTTGAGGAGCTGTTTGGAGCTTTATTCTGAGGCTGTTTCTGATCTGAAATCGGCTGTTTCTGCAGTGAAAATGGAGGATTACGAGACGGCGAAAGTGGAGGTGAGCGCCGCCATTGATGCACCTGTTAGTTGTGAAGATGGGTATAAGGAGAAGGATGGGGAGGTTTCTCCATTGACGGAGAAGAATGATGTGTTCTTCCATTTGGCTGCCATTTCTCTTGCGTTTGTTGATATG

Coding sequence (CDS)

AAGATGGAGAAGAAGCTCTTTCTTCTAATTCTACTGCTTTTCTCTCTCCTCTCACTTTGCCAATCGGAATCGGCGCCCGCAAACGACGTCGTTCCACGCTCCTGCAAGCAGGCCGCGACGCGAGACCCATACGTGGACTACAAACTCTGCGTCGAGGCCCTCAGGGCCAATCCCAAGGCCCGAAACGGTGCGTTTAAGGACCTGGTCCTGATCTCCATCGACCAAGCTAAGGCCAACGCCACGGAAATCGGGTCCGAGATTTCAGAGATTTTGAAGGGCAGCGGCAAATGGGGGACGAAGCCGAAGACCAAGACCAAGTATTCGTTAGATTGCTTGAGGAGCTGTTTGGAGCTTTATTCTGAGGCTGTTTCTGATCTGAAATCGGCTGTTTCTGCAGTGAAAATGGAGGATTACGAGACGGCGAAAGTGGAGGTGAGCGCCGCCATTGATGCACCTGTTAGTTGTGAAGATGGGTATAAGGAGAAGGATGGGGAGGTTTCTCCATTGACGGAGAAGAATGATGTGTTCTTCCATTTGGCTGCCATTTCTCTTGCGTTTGTTGATATG

Protein sequence

KMEKKLFLLILLLFSLLSLCQSESAPANDVVPRSCKQAATRDPYVDYKLCVEALRANPKARNGAFKDLVLISIDQAKANATEIGSEISEILKGSGKWGTKPKTKTKYSLDCLRSCLELYSEAVSDLKSAVSAVKMEDYETAKVEVSAAIDAPVSCEDGYKEKDGEVSPLTEKNDVFFHLAAISLAFVDM

Homology

[Close]

BLAST of MS005422 vs. NCBI nr
Match: XP_022159634.1 (putative invertase inhibitor [Momordica charantia])

HSP 1 Score: 358.2 bits (918), Expect = 4.3e-95
Identity = 186/188 (98.94%), Postives = 187/188 (99.47%), Query Frame = 0


Query: 2   MEKKLFLLILLLFSLLSLCQSESAPANDVVPRSCKQAATRDPYVDYKLCVEALRANPKAR 61
           MEKKLFLLILLLFSLLSLCQSESAPANDVV RSCKQAATRDPYVDYKLCVEALRANPKAR
Sbjct: 1   MEKKLFLLILLLFSLLSLCQSESAPANDVVQRSCKQAATRDPYVDYKLCVEALRANPKAR 60

Query: 62  NGAFKDLVLISIDQAKANATEIGSEISEILKGSGKWGTKPKTKTKYSLDCLRSCLELYSE 121
           NGAFKDLVL+SIDQAKANATEIGSEISEILKGSGKWGTKPKTKTKYSLDCLRSCLELYSE
Sbjct: 61  NGAFKDLVLVSIDQAKANATEIGSEISEILKGSGKWGTKPKTKTKYSLDCLRSCLELYSE 120

Query: 122 AVSDLKSAVSAVKMEDYETAKVEVSAAIDAPVSCEDGYKEKDGEVSPLTEKNDVFFHLAA 181
           AVSDLKSAVSAVKMEDYETAKVEVSAAIDAPVSCEDGYKEKDGEVSPLTEKNDVFFHLAA
Sbjct: 121 AVSDLKSAVSAVKMEDYETAKVEVSAAIDAPVSCEDGYKEKDGEVSPLTEKNDVFFHLAA 180

Query: 182 ISLAFVDM 190
           ISLAFVDM
Sbjct: 181 ISLAFVDM 188

[Close]

BLAST of MS005422 vs. NCBI nr
Match: XP_022958457.1 (putative invertase inhibitor [Cucurbita moschata])

HSP 1 Score: 227.3 bits (578), Expect = 1.1e-55
Identity = 122/180 (67.78%), Postives = 140/180 (77.78%), Query Frame = 0


Query: 10  ILLLFSLLSLCQSESAPANDVVPRSCKQAATRDPYVDYKLCVEALRANPKARNGAFKDLV 69
           IL+LFSLL+L Q       D+V RSCK AA RDPYVDYKLCV +LRANP +++  FKDLV
Sbjct: 9   ILMLFSLLTLFQ--FGFCLDIVQRSCKLAAKRDPYVDYKLCVRSLRANPSSKDATFKDLV 68

Query: 70  LISIDQAKANATEIGSEISEILKGSGKWGTKPKTKTKYSLDCLRSCLELYSEAVSDLKSA 129
           LISI+Q+KANAT IGSEI+ +LKG GKWG       KYS  CL SCLE+YSEAVSDLK  
Sbjct: 69  LISIEQSKANATAIGSEITGLLKGGGKWG-------KYSTSCLESCLEVYSEAVSDLKLG 128

Query: 130 VSAVKMEDYETAKVEVSAAIDAPVSCEDGYKEKDGEVSPLTEKNDVFFHLAAISLAFVDM 189
           + AVKM DYETA  EVSAA+DAP++CEDGY EKDGEVSPLTEKN  FF L AISLAF+ +
Sbjct: 129 LVAVKMGDYETANTEVSAAMDAPLTCEDGYNEKDGEVSPLTEKNGGFFQLTAISLAFISL 179

[Close]

BLAST of MS005422 vs. NCBI nr
Match: XP_022995887.1 (putative invertase inhibitor [Cucurbita maxima])

HSP 1 Score: 225.3 bits (573), Expect = 4.3e-55
Identity = 121/180 (67.22%), Postives = 142/180 (78.89%), Query Frame = 0


Query: 10  ILLLFSLLSLCQSESAPANDVVPRSCKQAATRDPYVDYKLCVEALRANPKARNGAFKDLV 69
           IL+LFSLL+L Q     + D+V RSCK AA RDPYVDYKLCV++LRANP +++  FKDLV
Sbjct: 9   ILVLFSLLTLFQ--FGFSLDIVQRSCKLAAKRDPYVDYKLCVKSLRANPSSKDATFKDLV 68

Query: 70  LISIDQAKANATEIGSEISEILKGSGKWGTKPKTKTKYSLDCLRSCLELYSEAVSDLKSA 129
           LISI+Q+KANAT IGSEI+ +LKG GKWG       KYS  CL SCLE+YSEAVSDLK  
Sbjct: 69  LISIEQSKANATAIGSEITGLLKGGGKWG-------KYSSSCLESCLEVYSEAVSDLKLG 128

Query: 130 VSAVKMEDYETAKVEVSAAIDAPVSCEDGYKEKDGEVSPLTEKNDVFFHLAAISLAFVDM 189
           + AVKM DYETA  EVSAA+DAP++CE+GY EKDGEVSPLTEKN  FF L AISLAF+ +
Sbjct: 129 LVAVKMGDYETANTEVSAAMDAPLTCENGYNEKDGEVSPLTEKNGGFFQLTAISLAFISL 179

[Close]

BLAST of MS005422 vs. NCBI nr
Match: KAG6606222.1 (hypothetical protein SDJN03_03539, partial [Cucurbita argyrosperma subsp. sororia] >KAG7036169.1 hypothetical protein SDJN02_02970, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 224.9 bits (572), Expect = 5.7e-55
Identity = 120/178 (67.42%), Postives = 139/178 (78.09%), Query Frame = 0


Query: 12  LLFSLLSLCQSESAPANDVVPRSCKQAATRDPYVDYKLCVEALRANPKARNGAFKDLVLI 71
           +LFSLL+L Q       D+V RSCK AA RDPYVDYKLCV++LRANP +++  FKDLVLI
Sbjct: 1   MLFSLLTLFQ--FGFCLDIVQRSCKLAAKRDPYVDYKLCVKSLRANPSSKDATFKDLVLI 60

Query: 72  SIDQAKANATEIGSEISEILKGSGKWGTKPKTKTKYSLDCLRSCLELYSEAVSDLKSAVS 131
           SI+Q+KANAT IGSEI+ +LKG GKWG       KYS  CL SCLE+YSEAVSDLK  + 
Sbjct: 61  SIEQSKANATAIGSEITGLLKGGGKWG-------KYSTSCLESCLEVYSEAVSDLKLGLV 120

Query: 132 AVKMEDYETAKVEVSAAIDAPVSCEDGYKEKDGEVSPLTEKNDVFFHLAAISLAFVDM 190
           AVKM DYETA  EVSAA+DAP++CEDGY EKDGEVSPLTEKN  FF L AISLAF+ +
Sbjct: 121 AVKMGDYETANTEVSAAMDAPLTCEDGYNEKDGEVSPLTEKNGGFFQLTAISLAFISL 169

[Close]

BLAST of MS005422 vs. NCBI nr
Match: XP_023534693.1 (putative invertase inhibitor [Cucurbita pepo subsp. pepo])

HSP 1 Score: 223.0 bits (567), Expect = 2.2e-54
Identity = 120/180 (66.67%), Postives = 141/180 (78.33%), Query Frame = 0


Query: 10  ILLLFSLLSLCQSESAPANDVVPRSCKQAATRDPYVDYKLCVEALRANPKARNGAFKDLV 69
           IL+L SLL+L Q     + D+V RSCK AA RDPYVDYKLCV++LRANP +++  FKDLV
Sbjct: 9   ILVLVSLLTLFQ--FGFSLDIVQRSCKLAAKRDPYVDYKLCVKSLRANPSSKDATFKDLV 68

Query: 70  LISIDQAKANATEIGSEISEILKGSGKWGTKPKTKTKYSLDCLRSCLELYSEAVSDLKSA 129
           LISI+Q+KANAT IGSEI+ +LKG GKWG       KYS  CL SCLE+YSEAVSDLK  
Sbjct: 69  LISIEQSKANATAIGSEITGLLKGGGKWG-------KYSTSCLESCLEVYSEAVSDLKLG 128

Query: 130 VSAVKMEDYETAKVEVSAAIDAPVSCEDGYKEKDGEVSPLTEKNDVFFHLAAISLAFVDM 189
           + AVKM DYETA  EVSAA+DAP++CE+GY EKDGEVSPLTEKN  FF L AISLAF+ +
Sbjct: 129 LVAVKMGDYETANTEVSAAMDAPLTCENGYNEKDGEVSPLTEKNGGFFQLTAISLAFISL 179

[Close]

BLAST of MS005422 vs. ExPASy Swiss-Prot
Match: Q8GT41 (Putative invertase inhibitor OS=Platanus acerifolia OX=140101 PE=1 SV=1)

HSP 1 Score: 109.8 bits (273), Expect = 3.5e-23
Identity = 72/186 (38.71%), Postives = 110/186 (59.14%), Query Frame = 0


Query: 4   KKLFLLILLLFSLLSLCQSESAPANDVVPRSCKQAATRDPYVDYKLCVEALRANPKARNG 63
           K  F L +  F+LL L Q  +  + D+V  +CK+ A R P V+Y  CV++L A+PK+   
Sbjct: 2   KLSFSLCIFFFNLLLLLQ--AVISADIVQGTCKKVAQRSPNVNYDFCVKSLGADPKSHTA 61

Query: 64  AFKDLVLISIDQAKANATEIGSEISEILKGSGKWGTKPKTKTKYSLDCLRSCLELYSEAV 123
             + L +IS + A  + ++I + I  ILK        P  K KY    L  C+ LY++A 
Sbjct: 62  DLQGLGVISANLAIQHGSKIQTFIGRILKSK----VDPALK-KY----LNDCVGLYADAK 121

Query: 124 SDLKSAVSAVKMEDYETAKVEVSAAIDAPVSCEDGYKEKDGEVSPLTEKNDVFFHLAAIS 183
           S ++ A++  K +DY +A V++SAA+D  V+CEDG+KEK G VSP+T++N  +  L AIS
Sbjct: 122 SSVQEAIADFKSKDYASANVKMSAALDDSVTCEDGFKEKKGIVSPVTKENKDYVQLTAIS 176

Query: 184 LAFVDM 190
           LA   +
Sbjct: 182 LAITKL 176

[Close]

BLAST of MS005422 vs. ExPASy Swiss-Prot
Match: A9YUH4 (Putative invertase inhibitor OS=Platanus orientalis OX=122832 PE=1 SV=1)

HSP 1 Score: 106.3 bits (264), Expect = 3.9e-22
Identity = 63/161 (39.13%), Postives = 97/161 (60.25%), Query Frame = 0


Query: 29  DVVPRSCKQAATRDPYVDYKLCVEALRANPKARNGAFKDLVLISIDQAKANATEIGSEIS 88
           D+V  +CK+ A R P V+Y  CV++L A+PK+ +   + L +IS + A    ++I + I 
Sbjct: 16  DIVQGTCKKVAQRSPNVNYDFCVKSLGADPKSHSADLQGLGVISANLAIQQGSKIQTFIG 75

Query: 89  EILKGSGKWGTKPKTKTKYSLDCLRSCLELYSEAVSDLKSAVSAVKMEDYETAKVEVSAA 148
            ILK        P  K KY    L  C+ LY++A S ++ A++  K +DY +A V++SAA
Sbjct: 76  RILKSK----VDPALK-KY----LNDCVGLYADAKSSVQEAIADFKSKDYASANVKMSAA 135

Query: 149 IDAPVSCEDGYKEKDGEVSPLTEKNDVFFHLAAISLAFVDM 190
           +D  V+CEDG+KEK G  SP+T++N  +  L AISLA   +
Sbjct: 136 LDDSVTCEDGFKEKKGIASPVTKENKDYVQLTAISLAITKL 167

[Close]

BLAST of MS005422 vs. ExPASy Swiss-Prot
Match: Q9FJR5 (Pectinesterase inhibitor 12 OS=Arabidopsis thaliana OX=3702 GN=PMEI12 PE=2 SV=1)

HSP 1 Score: 93.6 bits (231), Expect = 2.6e-18
Identity = 60/184 (32.61%), Postives = 101/184 (54.89%), Query Frame = 0


Query: 7   FLLILLLFSLLSLCQSESAPANDVVPRSCKQAATRDPYVDYKLCVEALRANPKARNG-AF 66
           FL+ L++FSL     +  A A  ++  SCK+A  +DP + Y  CV +L  +P+++     
Sbjct: 3   FLVSLVIFSLF---LNGFATAQTLIQDSCKKAFAKDPQLSYDFCVNSLTQDPQSKAATTL 62

Query: 67  KDLVLISIDQAKANATEIGSEISEILKGSGKWGTKPKTKTKYSLDCLRSCLELYSEAVSD 126
           + LVL S   A A  T +   +++ LK           + +  ++ L+ CL  Y++A  D
Sbjct: 63  ESLVLASTKTAAAKITNLKGIVAQDLKDQ---------RYQDIVEDLKLCLGFYNDANDD 122

Query: 127 LKSAVSAVKMEDYETAKVEVSAAIDAPVSCEDGYKEKDGEVSPLTEKNDVFFHLAAISLA 186
           L +A++ +K  DY+ A + +SAA+D P +CED +KE   + SP+T +N + F    I LA
Sbjct: 123 LTTALANIKSRDYQGANINLSAALDVPGNCEDDFKEAK-KTSPITNENSILFKTILIPLA 173

Query: 187 FVDM 190
           F +M
Sbjct: 183 FTNM 173

[Close]

BLAST of MS005422 vs. ExPASy Swiss-Prot
Match: Q9LUV1 (Pectinesterase inhibitor 2 OS=Arabidopsis thaliana OX=3702 GN=PMEI2 PE=1 SV=1)

HSP 1 Score: 50.4 bits (119), Expect = 2.5e-05
Identity = 39/141 (27.66%), Postives = 67/141 (47.52%), Query Frame = 0


Query: 50  CVEALRANPKARNGAFKDLVLISIDQAKANATEIGSEISEILKGSGKWGTKPKTKTKYSL 109
           C   +++NPK      + L  I+   A+ +A+E   +I  ++K +    T P  K  Y+ 
Sbjct: 43  CTSYMKSNPKTSGADLQTLANITFGSAQTSASEGFRKIQSLVKTA----TNPTMKKAYT- 102

Query: 110 DCLRSCLELYSEAVSDLKSAVSAVKMEDYETAKVEVSAAIDAPVSCEDGYKEKDGEVSPL 169
               SC++ Y  A+S L  A  ++   D +   ++VSAA++ P +CE      D +V P 
Sbjct: 103 ----SCVQHYKSAISSLNDAKQSLASGDGKGLNIKVSAAMEGPSTCEQDM--ADFKVDPS 162

Query: 170 TEKND-VFFHLAAISLAFVDM 190
             KN   F ++  I L   +M
Sbjct: 163 AVKNSGDFQNICGIVLVISNM 172

[Close]

BLAST of MS005422 vs. ExPASy Swiss-Prot
Match: Q0J8J8 (Pectinesterase inhibitor 28 OS=Oryza sativa subsp. japonica OX=39947 GN=PMEI28 PE=2 SV=1)

HSP 1 Score: 50.1 bits (118), Expect = 3.3e-05
Identity = 42/168 (25.00%), Postives = 74/168 (44.05%), Query Frame = 0


Query: 26  PANDVVPRSCKQA----ATRDPYVDYKLCVEALRANPKARNGAFKDLVLISIDQAKANAT 85
           PA+ VVP + + A    AT +    Y +CV AL A+P +     + L  I++  A ANA+
Sbjct: 47  PASPVVPVAPQAAALVRATCNSTAYYDVCVSALAADPSSTTADVRGLSAIAVSVAAANAS 106

Query: 86  EIGSEISEILKGSGKWGTKPKTKTKYSLDCLRSCLELYSEAVSDLKSAVSAVKMEDYETA 145
                 + +  G+                 LR+C   Y +A   L +A  ++  +DY+ A
Sbjct: 107 GAAQAAAALANGTAPLAAAAAGDGTVQA-LLRACAGKYGDARDALAAAKESMGQQDYDLA 166

Query: 146 KVEVSAAIDAPVSCEDGYKEKDGEVSP--LTEKNDVFFHLAAISLAFV 188
            V VSA  + P  C+  ++ +     P  L  + +    L +++L  +
Sbjct: 167 TVHVSAGAEYPQVCKALFRRQRPGAYPAELAAREEALNKLCSVALDII 213

[Close]

BLAST of MS005422 vs. ExPASy TrEMBL
Match: A0A6J1E2X6 (putative invertase inhibitor OS=Momordica charantia OX=3673 GN=LOC111025985 PE=4 SV=1)

HSP 1 Score: 358.2 bits (918), Expect = 2.1e-95
Identity = 186/188 (98.94%), Postives = 187/188 (99.47%), Query Frame = 0


Query: 2   MEKKLFLLILLLFSLLSLCQSESAPANDVVPRSCKQAATRDPYVDYKLCVEALRANPKAR 61
           MEKKLFLLILLLFSLLSLCQSESAPANDVV RSCKQAATRDPYVDYKLCVEALRANPKAR
Sbjct: 1   MEKKLFLLILLLFSLLSLCQSESAPANDVVQRSCKQAATRDPYVDYKLCVEALRANPKAR 60

Query: 62  NGAFKDLVLISIDQAKANATEIGSEISEILKGSGKWGTKPKTKTKYSLDCLRSCLELYSE 121
           NGAFKDLVL+SIDQAKANATEIGSEISEILKGSGKWGTKPKTKTKYSLDCLRSCLELYSE
Sbjct: 61  NGAFKDLVLVSIDQAKANATEIGSEISEILKGSGKWGTKPKTKTKYSLDCLRSCLELYSE 120

Query: 122 AVSDLKSAVSAVKMEDYETAKVEVSAAIDAPVSCEDGYKEKDGEVSPLTEKNDVFFHLAA 181
           AVSDLKSAVSAVKMEDYETAKVEVSAAIDAPVSCEDGYKEKDGEVSPLTEKNDVFFHLAA
Sbjct: 121 AVSDLKSAVSAVKMEDYETAKVEVSAAIDAPVSCEDGYKEKDGEVSPLTEKNDVFFHLAA 180

Query: 182 ISLAFVDM 190
           ISLAFVDM
Sbjct: 181 ISLAFVDM 188

[Close]

BLAST of MS005422 vs. ExPASy TrEMBL
Match: A0A6J1H1W1 (putative invertase inhibitor OS=Cucurbita moschata OX=3662 GN=LOC111459677 PE=4 SV=1)

HSP 1 Score: 227.3 bits (578), Expect = 5.5e-56
Identity = 122/180 (67.78%), Postives = 140/180 (77.78%), Query Frame = 0


Query: 10  ILLLFSLLSLCQSESAPANDVVPRSCKQAATRDPYVDYKLCVEALRANPKARNGAFKDLV 69
           IL+LFSLL+L Q       D+V RSCK AA RDPYVDYKLCV +LRANP +++  FKDLV
Sbjct: 9   ILMLFSLLTLFQ--FGFCLDIVQRSCKLAAKRDPYVDYKLCVRSLRANPSSKDATFKDLV 68

Query: 70  LISIDQAKANATEIGSEISEILKGSGKWGTKPKTKTKYSLDCLRSCLELYSEAVSDLKSA 129
           LISI+Q+KANAT IGSEI+ +LKG GKWG       KYS  CL SCLE+YSEAVSDLK  
Sbjct: 69  LISIEQSKANATAIGSEITGLLKGGGKWG-------KYSTSCLESCLEVYSEAVSDLKLG 128

Query: 130 VSAVKMEDYETAKVEVSAAIDAPVSCEDGYKEKDGEVSPLTEKNDVFFHLAAISLAFVDM 189
           + AVKM DYETA  EVSAA+DAP++CEDGY EKDGEVSPLTEKN  FF L AISLAF+ +
Sbjct: 129 LVAVKMGDYETANTEVSAAMDAPLTCEDGYNEKDGEVSPLTEKNGGFFQLTAISLAFISL 179

[Close]

BLAST of MS005422 vs. ExPASy TrEMBL
Match: A0A6J1K084 (putative invertase inhibitor OS=Cucurbita maxima OX=3661 GN=LOC111491280 PE=4 SV=1)

HSP 1 Score: 225.3 bits (573), Expect = 2.1e-55
Identity = 121/180 (67.22%), Postives = 142/180 (78.89%), Query Frame = 0


Query: 10  ILLLFSLLSLCQSESAPANDVVPRSCKQAATRDPYVDYKLCVEALRANPKARNGAFKDLV 69
           IL+LFSLL+L Q     + D+V RSCK AA RDPYVDYKLCV++LRANP +++  FKDLV
Sbjct: 9   ILVLFSLLTLFQ--FGFSLDIVQRSCKLAAKRDPYVDYKLCVKSLRANPSSKDATFKDLV 68

Query: 70  LISIDQAKANATEIGSEISEILKGSGKWGTKPKTKTKYSLDCLRSCLELYSEAVSDLKSA 129
           LISI+Q+KANAT IGSEI+ +LKG GKWG       KYS  CL SCLE+YSEAVSDLK  
Sbjct: 69  LISIEQSKANATAIGSEITGLLKGGGKWG-------KYSSSCLESCLEVYSEAVSDLKLG 128

Query: 130 VSAVKMEDYETAKVEVSAAIDAPVSCEDGYKEKDGEVSPLTEKNDVFFHLAAISLAFVDM 189
           + AVKM DYETA  EVSAA+DAP++CE+GY EKDGEVSPLTEKN  FF L AISLAF+ +
Sbjct: 129 LVAVKMGDYETANTEVSAAMDAPLTCENGYNEKDGEVSPLTEKNGGFFQLTAISLAFISL 179

[Close]

BLAST of MS005422 vs. ExPASy TrEMBL
Match: A0A6J1ICI2 (putative invertase inhibitor OS=Cucurbita maxima OX=3661 GN=LOC111471816 PE=4 SV=1)

HSP 1 Score: 216.9 bits (551), Expect = 7.5e-53
Identity = 118/181 (65.19%), Postives = 141/181 (77.90%), Query Frame = 0


Query: 9   LILLLFSLLSLCQSESAPANDVVPRSCKQAATRDPYVDYKLCVEALRANPKARNGAFKDL 68
           ++LLL SL +      A + D++ RSCK AA  DPYVDYKLCV+ LRANP ++   FK+L
Sbjct: 9   ILLLLLSLPT--PFHLAFSLDIIQRSCKSAAKTDPYVDYKLCVQTLRANPNSKAATFKEL 68

Query: 69  VLISIDQAKANATEIGSEISEILKGSGKWGTKPKTKTKYSLDCLRSCLELYSEAVSDLKS 128
           VLISI Q+KANATEIGSEISE+LKGS +WG     K KYSL CL+ CLE+YSEAVS L+ 
Sbjct: 69  VLISITQSKANATEIGSEISELLKGSERWG-----KGKYSLGCLKGCLEIYSEAVSGLEL 128

Query: 129 AVSAVKMEDYETAKVEVSAAIDAPVSCEDGYKEKDGEVSPLTEKNDVFFHLAAISLAFVD 188
           A+ AVKM DYETA ++VSAA+DA +SCEDGYKEKDGEVSPLTE N  FF L AISLAF++
Sbjct: 129 ALGAVKMGDYETANIKVSAAMDAALSCEDGYKEKDGEVSPLTEINCGFFQLNAISLAFIN 182

Query: 189 M 190
           M
Sbjct: 189 M 182

[Close]

BLAST of MS005422 vs. ExPASy TrEMBL
Match: A0A5A7TAE5 (Putative invertase inhibitor OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold455G004030 PE=4 SV=1)

HSP 1 Score: 216.5 bits (550), Expect = 9.8e-53
Identity = 122/189 (64.55%), Postives = 142/189 (75.13%), Query Frame = 0


Query: 2   MEKKLFLLILLLFSLLSLCQSESAPANDVVPRSCKQAATRDPYVDYKLCVEALRANPKAR 61
           ME  +   IL L  + +L Q   A   D+V   CK AA  DPYVDYKLCV+ L+ANP + 
Sbjct: 1   MENPIAKSILFLLCVPTLIQ--LANGLDIVQHYCKLAAKTDPYVDYKLCVQTLKANPNSE 60

Query: 62  NGAFKDLVLISIDQAKANATEIGSEISEILK-GSGKWGTKPKTKTKYSLDCLRSCLELYS 121
           +  FKDLV+ISI+QAKANATEI SEISE++K  S KWG       KYSL+CL+SCLELYS
Sbjct: 61  DADFKDLVMISINQAKANATEIRSEISELMKRTSEKWG-------KYSLNCLKSCLELYS 120

Query: 122 EAVSDLKSAVSAVKMEDYETAKVEVSAAIDAPVSCEDGYKEKDGEVSPLTEKNDVFFHLA 181
           EAVSDLK A+  +KMEDYETAK  VSAA+DAPVSCEDGYKEKDGEVSPL+E ND FF L 
Sbjct: 121 EAVSDLKKALRGLKMEDYETAKTAVSAAMDAPVSCEDGYKEKDGEVSPLSETNDGFFQLV 180

Query: 182 AISLAFVDM 190
           AISLAF++M
Sbjct: 181 AISLAFINM 180

[Close]

BLAST of MS005422 vs. TAIR 10
Match: AT5G46930.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 108.2 bits (269), Expect = 7.2e-24
Identity = 69/186 (37.10%), Postives = 105/186 (56.45%), Query Frame = 0


Query: 7   FLLILLLFSLLSLCQSESAPANDVVPRSCKQAATRDPYVDYKLCVEALRANPKARNG-AF 66
           F L L++F LL  C    A A  ++  SCK+A T+DP + Y  CV++L  NP+++   + 
Sbjct: 3   FSLYLVMFFLLLNC---FATAQSLIRDSCKKATTKDPKLKYDFCVKSLEENPQSKTAKSL 62

Query: 67  KDLVLISIDQAKANATEIGSEISEILKGSGKWGTKPKTKTKYSLD-CLRSCLELYSEAVS 126
           + LV +S   A +  T +   + +ILK           + KY ++  L  CLELY+EA+ 
Sbjct: 63  EGLVFVSTKNAVSKTTSLKGMVDKILK-----------EDKYEVERPLLDCLELYTEAID 122

Query: 127 DLKSAVSAVKMEDYETAKVEVSAAIDAPVSCEDGY-KEKDGEVSPLTEKNDVFFHLAAIS 186
            L  ++  VK  DY+TA + +SAA+DAP SCE  + K K    SP T++NDV F++  I 
Sbjct: 123 SLNQSLDTVKSRDYKTATMLMSAAMDAPGSCETKFTKRKKAVKSPFTKENDVLFYMVLIP 174

Query: 187 LAFVDM 190
           +AF  M
Sbjct: 183 IAFTSM 174

[Close]

BLAST of MS005422 vs. TAIR 10
Match: AT5G46940.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 104.0 bits (258), Expect = 1.4e-22
Identity = 66/184 (35.87%), Postives = 99/184 (53.80%), Query Frame = 0


Query: 7   FLLILLLFSLLSLCQSESAPANDVVPRSCKQAATRDPYVDYKLCVEALRANPKARNGA-F 66
           FLL L+ F +LS   +  A    ++  SCK+A    P   Y LCV +L  NP+A+     
Sbjct: 3   FLLYLVTFFVLS---NGLANGQTLIRNSCKKATATSPKFKYNLCVTSLETNPQAKTAKDL 62

Query: 67  KDLVLISIDQAKANATEIGSEISEILKGSGKWGTKPKTKTKYSLDCLRSCLELYSEAVSD 126
             LV+ S   A   AT +   + +I+KG        K   K +   LR CL+LY++A+  
Sbjct: 63  AGLVMASTKNAVTKATTLKGTVDKIIKG--------KKVNKMTAMPLRDCLQLYTDAIGS 122

Query: 127 LKSAVSAVKMEDYETAKVEVSAAIDAPVSCEDGYKEKDGEVSPLTEKNDVFFHLAAISLA 186
           L  A++ VK  +Y T K  +SAA+D P +CE G+KE+    SP+T++ND  + +  I LA
Sbjct: 123 LNEALAGVKSRNYPTVKTVLSAAMDTPSTCETGFKERKAP-SPVTKENDNLYQMILIPLA 174

Query: 187 FVDM 190
           F +M
Sbjct: 183 FTNM 174

[Close]

BLAST of MS005422 vs. TAIR 10
Match: AT5G46970.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 99.4 bits (246), Expect = 3.3e-21
Identity = 63/172 (36.63%), Postives = 94/172 (54.65%), Query Frame = 0


Query: 8   LLILLLFSLLSLCQSESAPANDVVPRSCKQAATRDPYVDYKLCVEALRANPKARNGAFKD 67
           +  L  F++  L  +    A  ++   CK+AA ++P + Y  CV++L  NP+++     D
Sbjct: 1   MTFLFYFAMFFLLLNAFTIAQSLIQDFCKKAADKNPKIHYNFCVKSLEENPQSKTARSLD 60

Query: 68  -LVLISIDQAKANATEIGSEISEILKGSGKWGTKPKTKTKYSLDCLRSCLELYSEAVSDL 127
            LV++S   A +  T +   + +IL        K      YS   LR CLELYS+A + L
Sbjct: 61  RLVMLSTKNAVSKTTSMKGIVDKIL--------KENRFEMYSEKPLRDCLELYSDATNSL 120

Query: 128 KSAVSAVKMEDYETAKVEVSAAIDAPVSCEDGYKE-KDGEVSPLTEKNDVFF 178
           K A++ +K  DY+TA V +SAA+ AP SCE G+KE K    SP T+ NDV F
Sbjct: 121 KEALTIIKSRDYKTANVVISAAMGAPPSCEIGFKEGKKPLKSPFTKDNDVLF 164

[Close]

BLAST of MS005422 vs. TAIR 10
Match: AT5G46960.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 93.6 bits (231), Expect = 1.8e-19
Identity = 60/184 (32.61%), Postives = 101/184 (54.89%), Query Frame = 0


Query: 7   FLLILLLFSLLSLCQSESAPANDVVPRSCKQAATRDPYVDYKLCVEALRANPKARNG-AF 66
           FL+ L++FSL     +  A A  ++  SCK+A  +DP + Y  CV +L  +P+++     
Sbjct: 3   FLVSLVIFSLF---LNGFATAQTLIQDSCKKAFAKDPQLSYDFCVNSLTQDPQSKAATTL 62

Query: 67  KDLVLISIDQAKANATEIGSEISEILKGSGKWGTKPKTKTKYSLDCLRSCLELYSEAVSD 126
           + LVL S   A A  T +   +++ LK           + +  ++ L+ CL  Y++A  D
Sbjct: 63  ESLVLASTKTAAAKITNLKGIVAQDLKDQ---------RYQDIVEDLKLCLGFYNDANDD 122

Query: 127 LKSAVSAVKMEDYETAKVEVSAAIDAPVSCEDGYKEKDGEVSPLTEKNDVFFHLAAISLA 186
           L +A++ +K  DY+ A + +SAA+D P +CED +KE   + SP+T +N + F    I LA
Sbjct: 123 LTTALANIKSRDYQGANINLSAALDVPGNCEDDFKEAK-KTSPITNENSILFKTILIPLA 173

Query: 187 FVDM 190
           F +M
Sbjct: 183 FTNM 173

[Close]

BLAST of MS005422 vs. TAIR 10
Match: AT5G46950.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 89.0 bits (219), Expect = 4.5e-18
Identity = 58/184 (31.52%), Postives = 99/184 (53.80%), Query Frame = 0


Query: 7   FLLILLLFSLLSLCQSESAPANDVVPRSCKQAATRDPYVDYKLCVEALRANPKARNG-AF 66
           FL+ L++FSL     +  A A  ++  SCK+A  +DP   Y  CV++L  +P+++     
Sbjct: 3   FLVSLVIFSLF---LNGFATAQTLIQDSCKKAFAKDPQSSYDFCVQSLTQDPQSKAATTL 62

Query: 67  KDLVLISIDQAKANATEIGSEISEILKGSGKWGTKPKTKTKYSLDCLRSCLELYSEAVSD 126
           +DL L S     A  T +   +++ LK           + +  ++ L+ CL  Y +A   
Sbjct: 63  EDLALASTKNVAAKITNLKGIVAQDLKDQ---------RYQDIVEDLKLCLGFYKDANDS 122

Query: 127 LKSAVSAVKMEDYETAKVEVSAAIDAPVSCEDGYKEKDGEVSPLTEKNDVFFHLAAISLA 186
           LK+A++ +K  DY+ A   +SAA++AP  CED +KE + + SP+T +N++ +    I LA
Sbjct: 123 LKTALANIKSRDYDGANSNLSAALNAPGDCEDDFKEAE-KKSPITNENNILYKTIVIPLA 173

Query: 187 FVDM 190
           F  M
Sbjct: 183 FTTM 173

The following BLAST results are available for this feature:

BLAST of MS005422 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022159634.1	4.3e-95	98.94	putative invertase inhibitor [Momordica charantia]	[more]
XP_022958457.1	1.1e-55	67.78	putative invertase inhibitor [Cucurbita moschata]	[more]
XP_022995887.1	4.3e-55	67.22	putative invertase inhibitor [Cucurbita maxima]	[more]
KAG6606222.1	5.7e-55	67.42	hypothetical protein SDJN03_03539, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_023534693.1	2.2e-54	66.67	putative invertase inhibitor [Cucurbita pepo subsp. pepo]	[more]

BLAST of MS005422 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8GT41	3.5e-23	38.71	Putative invertase inhibitor OS=Platanus acerifolia OX=140101 PE=1 SV=1	[more]
A9YUH4	3.9e-22	39.13	Putative invertase inhibitor OS=Platanus orientalis OX=122832 PE=1 SV=1	[more]
Q9FJR5	2.6e-18	32.61	Pectinesterase inhibitor 12 OS=Arabidopsis thaliana OX=3702 GN=PMEI12 PE=2 SV=1	[more]
Q9LUV1	2.5e-05	27.66	Pectinesterase inhibitor 2 OS=Arabidopsis thaliana OX=3702 GN=PMEI2 PE=1 SV=1	[more]
Q0J8J8	3.3e-05	25.00	Pectinesterase inhibitor 28 OS=Oryza sativa subsp. japonica OX=39947 GN=PMEI28 P...	[more]

BLAST of MS005422 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1E2X6	2.1e-95	98.94	putative invertase inhibitor OS=Momordica charantia OX=3673 GN=LOC111025985 PE=4...	[more]
A0A6J1H1W1	5.5e-56	67.78	putative invertase inhibitor OS=Cucurbita moschata OX=3662 GN=LOC111459677 PE=4 ...	[more]
A0A6J1K084	2.1e-55	67.22	putative invertase inhibitor OS=Cucurbita maxima OX=3661 GN=LOC111491280 PE=4 SV...	[more]
A0A6J1ICI2	7.5e-53	65.19	putative invertase inhibitor OS=Cucurbita maxima OX=3661 GN=LOC111471816 PE=4 SV...	[more]
A0A5A7TAE5	9.8e-53	64.55	Putative invertase inhibitor OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca...	[more]

BLAST of MS005422 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G46930.1	7.2e-24	37.10	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT5G46940.1	1.4e-22	35.87	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT5G46970.1	3.3e-21	36.63	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT5G46960.1	1.8e-19	32.61	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT5G46950.1	4.5e-18	31.52	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 26..186 e-value: 1.9E-27 score: 107.1
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 31..185 e-value: 7.0E-28 score: 97.9
IPR006501	Pectinesterase inhibitor domain	TIGRFAM	TIGR01614	TIGR01614	coord: 8..189 e-value: 8.5E-33 score: 111.1
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	GENE3D	1.20.140.40	Invertase/pectin methylesterase inhibitor family protein	coord: 26..189 e-value: 4.2E-33 score: 116.4
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	SUPERFAMILY	101148	Plant invertase/pectin methylesterase inhibitor	coord: 28..189
None	No IPR available	PANTHER	PTHR31080	PECTINESTERASE INHIBITOR-LIKE	coord: 11..188
None	No IPR available	PANTHER	PTHR31080:SF239	PLANT INVERTASE/PECTIN METHYLESTERASE INHIBITOR SUPERFAMILY PROTEIN	coord: 11..188
IPR034088	Pla a 1-like	CDD	cd15795	PMEI-Pla_a_1_like	coord: 35..189 e-value: 4.67144E-42 score: 136.726

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS005422.1	MS005422.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0004857	enzyme inhibitor activity