MS005114 (gene) Bitter gourd (TR) v1

Overview
NameMS005114
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
Descriptiontranscription factor FER-LIKE IRON DEFICIENCY-INDUCED TRANSCRIPTION FACTOR
Locationscaffold176: 2794955 .. 2796140 (+)
RNA-Seq ExpressionMS005114
SyntenyMS005114
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGCCAATTGGGAAGCCATTAATGATGAACATCAACGACTTCGAGCTCCAAGATTTCATTCACGGCGACAACTTTGATCAATACGTGAATCTCATTCGTGGGGAGCCCGAAAATCCAACATTCAACTTTGATCTTGAATACATGCTAGATTCCAATATTGTCATGAATTCTGATCCCAATTCTCTGTTCGGTTCATTGGAGAGTTTCAACAGTATTATGAGAGAAGTTGAAGATGAAGACGAAGACGATACTTCTGTGGAGAATTCTTCCATCACAACAACAACAAAGAAGCCAAAAGTTGACAGATCAAGAACTTTGATTTCTGAAAGAAGGAGGAGGGGAAGAATGAAGGAAAAACTTTATGCATTACGCTCATTGGTTCCAAATATAACCAAGGTATGGCAATTGGCAAATACCCACATTTAGAATCATAATTCAATTTGTCCAATGATTTGATATTGGATAGAAAATTTGTGAAAATGTAGATGGATAAGGCTTCCATTGTTGGAGATGCAGTGTTGTATGTGAAAGAGCTTCAAATGCAGGCCAAGAAGCTCAAAGCTGAGATATCAGTTTTGGAATCCTCCATTAATGAGATACAAACACACCATCGACCAACTGATCAAAGCCACAGAAAGATTCAAACTTCACGCTCTGACCAGTTCCTCCCCAACAAGATAATTCAGGCAAGTTTTTAGGAAATTTCATGTTCTGTTCTCTGTAGTGATGTTCTGTTTTTTTCAGCTGCTAATTTGGAATCTTGGGTTTCAGCTGGATGTGTTTCAAGTGGAGGAAAGAGGGTTTTATTTGAGGATGGTGTGCAAGATGGGTGAAGGAGTAGCCATGTCCCTTTACAAGGTTCTTGAATCTCTTACCAGTTTCAATATCCAAAGCTCCAACTTGGCCTCTGCCTCTGACAGATTCATCCTCACTGCTACAATCAATGTGATTACCTTCTTATGATTTGAATTGAAATGACTTGAAAAGAGGATCAAAATTGTCCAACCTTTTTCATGTCAAGTTTTAAAATGTTTCTTGAGATGGGAATTTTTGGTTGCTTCCCATTTTCTGATGTTTTGGCATTGTAATGTTTGTGCAGGTGAGAGATTGTGAGGTGGACATGAACTTGCCAAATATGAAGCTTTGGCTTACTGGGGCACTTCTTAACCATGGATTTGAGTTC

mRNA sequence

ATGGAGCCAATTGGGAAGCCATTAATGATGAACATCAACGACTTCGAGCTCCAAGATTTCATTCACGGCGACAACTTTGATCAATACGTGAATCTCATTCGTGGGGAGCCCGAAAATCCAACATTCAACTTTGATCTTGAATACATGCTAGATTCCAATATTGTCATGAATTCTGATCCCAATTCTCTGTTCGGTTCATTGGAGAGTTTCAACAGTATTATGAGAGAAGTTGAAGATGAAGACGAAGACGATACTTCTGTGGAGAATTCTTCCATCACAACAACAACAAAGAAGCCAAAAGTTGACAGATCAAGAACTTTGATTTCTGAAAGAAGGAGGAGGGGAAGAATGAAGGAAAAACTTTATGCATTACGCTCATTGGTTCCAAATATAACCAAGATGGATAAGGCTTCCATTGTTGGAGATGCAGTGTTGTATGTGAAAGAGCTTCAAATGCAGGCCAAGAAGCTCAAAGCTGAGATATCAGTTTTGGAATCCTCCATTAATGAGATACAAACACACCATCGACCAACTGATCAAAGCCACAGAAAGATTCAAACTTCACGCTCTGACCAGTTCCTCCCCAACAAGATAATTCAGGCAATGATGTTCTGTTTTTTTCAGCTGCTAATTTGGAATCTTGGGTTTCAGCTGGATGTGTTTCAAGTGGAGGAAAGAGGGTTTTATTTGAGGATGGTGTGCAAGATGGGTGAAGGAGTAGCCATGTCCCTTTACAAGGTTCTTGAATCTCTTACCAGTTTCAATATCCAAAGCTCCAACTTGGCCTCTGCCTCTGACAGATTCATCCTCACTGCTACAATCAATGTGAGAGATTGTGAGGTGGACATGAACTTGCCAAATATGAAGCTTTGGCTTACTGGGGCACTTCTTAACCATGGATTTGAGTTC

Coding sequence (CDS)

ATGGAGCCAATTGGGAAGCCATTAATGATGAACATCAACGACTTCGAGCTCCAAGATTTCATTCACGGCGACAACTTTGATCAATACGTGAATCTCATTCGTGGGGAGCCCGAAAATCCAACATTCAACTTTGATCTTGAATACATGCTAGATTCCAATATTGTCATGAATTCTGATCCCAATTCTCTGTTCGGTTCATTGGAGAGTTTCAACAGTATTATGAGAGAAGTTGAAGATGAAGACGAAGACGATACTTCTGTGGAGAATTCTTCCATCACAACAACAACAAAGAAGCCAAAAGTTGACAGATCAAGAACTTTGATTTCTGAAAGAAGGAGGAGGGGAAGAATGAAGGAAAAACTTTATGCATTACGCTCATTGGTTCCAAATATAACCAAGATGGATAAGGCTTCCATTGTTGGAGATGCAGTGTTGTATGTGAAAGAGCTTCAAATGCAGGCCAAGAAGCTCAAAGCTGAGATATCAGTTTTGGAATCCTCCATTAATGAGATACAAACACACCATCGACCAACTGATCAAAGCCACAGAAAGATTCAAACTTCACGCTCTGACCAGTTCCTCCCCAACAAGATAATTCAGGCAATGATGTTCTGTTTTTTTCAGCTGCTAATTTGGAATCTTGGGTTTCAGCTGGATGTGTTTCAAGTGGAGGAAAGAGGGTTTTATTTGAGGATGGTGTGCAAGATGGGTGAAGGAGTAGCCATGTCCCTTTACAAGGTTCTTGAATCTCTTACCAGTTTCAATATCCAAAGCTCCAACTTGGCCTCTGCCTCTGACAGATTCATCCTCACTGCTACAATCAATGTGAGAGATTGTGAGGTGGACATGAACTTGCCAAATATGAAGCTTTGGCTTACTGGGGCACTTCTTAACCATGGATTTGAGTTC

Protein sequence

MEPIGKPLMMNINDFELQDFIHGDNFDQYVNLIRGEPENPTFNFDLEYMLDSNIVMNSDPNSLFGSLESFNSIMREVEDEDEDDTSVENSSITTTTKKPKVDRSRTLISERRRRGRMKEKLYALRSLVPNITKMDKASIVGDAVLYVKELQMQAKKLKAEISVLESSINEIQTHHRPTDQSHRKIQTSRSDQFLPNKIIQAMMFCFFQLLIWNLGFQLDVFQVEERGFYLRMVCKMGEGVAMSLYKVLESLTSFNIQSSNLASASDRFILTATINVRDCEVDMNLPNMKLWLTGALLNHGFEF
Homology
BLAST of MS005114 vs. NCBI nr
Match: XP_022140694.1 (transcription factor FER-LIKE IRON DEFICIENCY-INDUCED TRANSCRIPTION FACTOR [Momordica charantia])

HSP 1 Score: 542.3 bits (1396), Expect = 2.6e-150
Identity = 286/303 (94.39%), Postives = 286/303 (94.39%), Query Frame = 0

Query: 1   MEPIGKPLMMNINDFELQDFIHGDNFDQYVNLIRGEPENPTFNFDLEYMLDSNIVMNSDP 60
           MEPIGKPLMMNINDFELQDFIHGDNFDQYVNLIRGEPENPTFNFDLEYMLDSNIVMNSDP
Sbjct: 4   MEPIGKPLMMNINDFELQDFIHGDNFDQYVNLIRGEPENPTFNFDLEYMLDSNIVMNSDP 63

Query: 61  NSLFGSLESFNSIMREVEDEDEDDTSVENSSITTTTKKPKVDRSRTLISERRRRGRMKEK 120
           NSLFGSLESFNSIMREVEDEDEDDTSVENSSITTTTKKPKVDRSRTLISERRRRGRMKEK
Sbjct: 64  NSLFGSLESFNSIMREVEDEDEDDTSVENSSITTTTKKPKVDRSRTLISERRRRGRMKEK 123

Query: 121 LYALRSLVPNITKMDKASIVGDAVLYVKELQMQAKKLKAEISVLESSINEIQTHHRPTDQ 180
           LYALRSLVPNITKMDKASIVGDAVLYVKELQMQAKKLKAEISVLESSINEIQTHHRPTDQ
Sbjct: 124 LYALRSLVPNITKMDKASIVGDAVLYVKELQMQAKKLKAEISVLESSINEIQTHHRPTDQ 183

Query: 181 SHRKIQTSRSDQFLPNKIIQAMMFCFFQLLIWNLGFQLDVFQVEERGFYLRMVCKMGEGV 240
           SHRKIQTSRSDQFLPNKII                 QLDVFQVEERGFYLRMVCKMGEGV
Sbjct: 184 SHRKIQTSRSDQFLPNKII-----------------QLDVFQVEERGFYLRMVCKMGEGV 243

Query: 241 AMSLYKVLESLTSFNIQSSNLASASDRFILTATINVRDCEVDMNLPNMKLWLTGALLNHG 300
           AMSLYKVLESLTSFNIQSSNLASASDRFILTATINVRDCEVDMNLPNMKLWLTGALLNHG
Sbjct: 244 AMSLYKVLESLTSFNIQSSNLASASDRFILTATINVRDCEVDMNLPNMKLWLTGALLNHG 289

Query: 301 FEF 304
           FEF
Sbjct: 304 FEF 289

BLAST of MS005114 vs. NCBI nr
Match: XP_022943777.1 (transcription factor FER-LIKE IRON DEFICIENCY-INDUCED TRANSCRIPTION FACTOR-like [Cucurbita moschata] >XP_023513372.1 transcription factor FER-LIKE IRON DEFICIENCY-INDUCED TRANSCRIPTION FACTOR [Cucurbita pepo subsp. pepo] >KAG6570822.1 Transcription factor FER-LIKE IRON DEFICIENCY-INDUCED TRANSCRIPTION FACTOR, partial [Cucurbita argyrosperma subsp. sororia] >KAG7010664.1 Transcription factor FER-LIKE IRON DEFICIENCY-INDUCED TRANSCRIPTION FACTOR [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 443.0 bits (1138), Expect = 2.1e-120
Identity = 254/320 (79.38%), Postives = 268/320 (83.75%), Query Frame = 0

Query: 1   MEPIGKPLMMNINDFELQDFIHGDNFDQYVNLIRGEPENPTF-NFDL------------- 60
           ME +GK LMMNIN+FELQDFIHGDNFDQYVNLIRG  ENP F N DL             
Sbjct: 1   MEHVGKHLMMNINEFELQDFIHGDNFDQYVNLIRGGSENPIFDNLDLDFMNVDNRLDPSA 60

Query: 61  EYMLDSNIVMNSDPNSLFGSLESFNSIMREV--EDEDEDDTSVENSSITTTTKKPKVDRS 120
           EY+LDSNIVMNSDPNSLF SLESFNSIM+EV  EDEDEDDTSVENSS+ TT+KK KVDRS
Sbjct: 61  EYILDSNIVMNSDPNSLFSSLESFNSIMKEVENEDEDEDDTSVENSSV-TTSKKSKVDRS 120

Query: 121 RTLISERRRRGRMKEKLYALRSLVPNITKMDKASIVGDAVLYVKELQMQAKKLKAEISVL 180
           RTLISERRRRGRMKEKLYALRSLVPNITKMDKASIVGDAVLYVKELQ+QAKKLKAEISVL
Sbjct: 121 RTLISERRRRGRMKEKLYALRSLVPNITKMDKASIVGDAVLYVKELQVQAKKLKAEISVL 180

Query: 181 ESSINEIQTHHRPTDQSHRKI-QTSRSDQFLPNKIIQAMMFCFFQLLIWNLGFQLDVFQV 240
           ESSINE QT HR   Q+H+KI QTS S QFLP KII                 QLDVFQV
Sbjct: 181 ESSINETQTSHR--GQNHKKIVQTSHSGQFLPCKII-----------------QLDVFQV 240

Query: 241 EERGFYLRMVCKMGEGVAMSLYKVLESLTSFNIQSSNLASASDRFILTATINVRDCEVDM 300
           EERGFYLR+VCKMGE VAMSLYKVLESLTSF+IQSSNLASASDRFILTATINVR+CE+DM
Sbjct: 241 EERGFYLRLVCKMGERVAMSLYKVLESLTSFSIQSSNLASASDRFILTATINVRNCEMDM 300

Query: 301 NLPNMKLWLTGALLNHGFEF 304
           NLPN+KLWLTGALLNHGFEF
Sbjct: 301 NLPNLKLWLTGALLNHGFEF 300

BLAST of MS005114 vs. NCBI nr
Match: XP_022986352.1 (transcription factor FER-LIKE IRON DEFICIENCY-INDUCED TRANSCRIPTION FACTOR-like [Cucurbita maxima])

HSP 1 Score: 434.5 bits (1116), Expect = 7.6e-118
Identity = 250/320 (78.12%), Postives = 265/320 (82.81%), Query Frame = 0

Query: 1   MEPIGKPLMMNINDFELQDFIHGDNFDQYVNLIRGEPENPTF-NFDL------------- 60
           ME +GK LMMNIN+FELQD IHGDNFDQYVNLIRG  ENP F N DL             
Sbjct: 1   MEHVGKHLMMNINEFELQDLIHGDNFDQYVNLIRGGSENPIFDNLDLDFMNVDNRLDPSA 60

Query: 61  EYMLDSNIVMNSDPNSLFGSLESFNSIMREV--EDEDEDDTSVENSSITTTTKKPKVDRS 120
           EY+LDSNIVMNSDPNSLF SLESFNSIM+EV  EDEDEDD SVENSS+ TT+KK KVDRS
Sbjct: 61  EYILDSNIVMNSDPNSLFSSLESFNSIMKEVETEDEDEDDASVENSSV-TTSKKSKVDRS 120

Query: 121 RTLISERRRRGRMKEKLYALRSLVPNITKMDKASIVGDAVLYVKELQMQAKKLKAEISVL 180
           RTLISERRRRGRMKEKLYALRSLVPNITKMDKASIVGDAVLYVKELQ+QAKKLKAEISVL
Sbjct: 121 RTLISERRRRGRMKEKLYALRSLVPNITKMDKASIVGDAVLYVKELQVQAKKLKAEISVL 180

Query: 181 ESSINEIQTHHRPTDQSHRKI-QTSRSDQFLPNKIIQAMMFCFFQLLIWNLGFQLDVFQV 240
           ESSINE QT +R   Q+H+ I QTS S QFLP KII                 QLDVFQV
Sbjct: 181 ESSINETQTSYR--GQNHKNIVQTSHSGQFLPCKII-----------------QLDVFQV 240

Query: 241 EERGFYLRMVCKMGEGVAMSLYKVLESLTSFNIQSSNLASASDRFILTATINVRDCEVDM 300
           EERGFYLR+VCKMGE VAMSLYKVLESLTSF+IQSSNLASASDRFILTATINVR+CE+DM
Sbjct: 241 EERGFYLRLVCKMGERVAMSLYKVLESLTSFSIQSSNLASASDRFILTATINVRNCEMDM 300

Query: 301 NLPNMKLWLTGALLNHGFEF 304
           NLPN+KLWLTGALLNHGFEF
Sbjct: 301 NLPNLKLWLTGALLNHGFEF 300

BLAST of MS005114 vs. NCBI nr
Match: XP_038902250.1 (transcription factor FER-LIKE IRON DEFICIENCY-INDUCED TRANSCRIPTION FACTOR [Benincasa hispida])

HSP 1 Score: 422.9 bits (1086), Expect = 2.3e-114
Identity = 246/325 (75.69%), Postives = 263/325 (80.92%), Query Frame = 0

Query: 1   MEPIGKPLMMNIND-FELQDFIHGDNFDQYVNLIRGEPENPTF-NFD------------- 60
           ME IG+ LM NI+D FELQDFIHGDNFDQYVNLIRG  EN  F NFD             
Sbjct: 1   MEQIGRHLMTNISDQFELQDFIHGDNFDQYVNLIRGGHENQIFNNFDPDFMNSLLVENGA 60

Query: 61  ---LEYMLDSNIVM--NSDPNSLFGSLESFNSIMREVE--DEDEDDTSVENSSITTTTKK 120
               E+++DSN+VM  NSDPNSLF +LESFNSIM+EVE  DEDEDDTSVENSS  T +KK
Sbjct: 61  DPSAEFIVDSNMVMNNNSDPNSLFSTLESFNSIMKEVENDDEDEDDTSVENSSSFTNSKK 120

Query: 121 PKVDRSRTLISERRRRGRMKEKLYALRSLVPNITKMDKASIVGDAVLYVKELQMQAKKLK 180
           PKVDR+RTLISERRRRGRMKEKLYALRSLVPNITKMDKASIVGDAVLYVKELQMQAKKLK
Sbjct: 121 PKVDRTRTLISERRRRGRMKEKLYALRSLVPNITKMDKASIVGDAVLYVKELQMQAKKLK 180

Query: 181 AEISVLESSINEIQTHHRPTDQSHRKIQTSRSDQFLPNKIIQAMMFCFFQLLIWNLGFQL 240
           AEISVLESSINE QT+HR  +Q +  IQT  S+QFLP KII                 QL
Sbjct: 181 AEISVLESSINETQTNHRLQNQKN-IIQTPHSEQFLPTKII-----------------QL 240

Query: 241 DVFQVEERGFYLRMVCKMGEGVAMSLYKVLESLTSFNIQSSNLASASDRFILTATINVRD 300
           DVFQVEERGFYLR+VCKMGE VAMSLYKVLESLTSF IQSSNLASASDRFILTATINVRD
Sbjct: 241 DVFQVEERGFYLRLVCKMGERVAMSLYKVLESLTSFIIQSSNLASASDRFILTATINVRD 300

Query: 301 CEVDMNLPNMKLWLTGALLNHGFEF 304
           CEV+MNLPN+KLWLTGALLNHGFEF
Sbjct: 301 CEVEMNLPNLKLWLTGALLNHGFEF 307

BLAST of MS005114 vs. NCBI nr
Match: XP_004145819.1 (transcription factor FER-LIKE IRON DEFICIENCY-INDUCED TRANSCRIPTION FACTOR [Cucumis sativus] >KAE8646895.1 hypothetical protein Csa_020756 [Cucumis sativus])

HSP 1 Score: 422.5 bits (1085), Expect = 3.0e-114
Identity = 247/327 (75.54%), Postives = 263/327 (80.43%), Query Frame = 0

Query: 1   MEPIGKPLMMNIND-FELQDFIHGDNFDQYVNLIRGEPENPTF--NFD------------ 60
           ME  G+ LM +IND FELQDFIHGDNFDQYVNLIRG  E P F  NFD            
Sbjct: 1   MEQFGRHLMTDINDQFELQDFIHGDNFDQYVNLIRGGNETPIFNNNFDLDFMNGCLIENR 60

Query: 61  -----LEYMLDSNIVMNSDPNSLFGSLESFNSIMREVEDEDEDD---TSVENSSITTTTK 120
                LEY++DSN+VMNSDPNSLF +LESFNSIM+EVEDEDED+    SVENSS +TT+K
Sbjct: 61  VVDQSLEYIVDSNMVMNSDPNSLFSTLESFNSIMKEVEDEDEDEDENESVENSS-STTSK 120

Query: 121 KPKVDRSRTLISERRRRGRMKEKLYALRSLVPNITKMDKASIVGDAVLYVKELQMQAKKL 180
           KPK DR+RTLISERRRRGRMKEKLYALRSLVPNITKMDKASIVGDAVLYVKELQMQAKKL
Sbjct: 121 KPKADRTRTLISERRRRGRMKEKLYALRSLVPNITKMDKASIVGDAVLYVKELQMQAKKL 180

Query: 181 KAEISVLESSINEIQTHHRPTDQSHRK-IQTSRSDQFLPNKIIQAMMFCFFQLLIWNLGF 240
           K+EISVLESSINE Q  HR  DQ+ +K IQTS SDQFLP KII                 
Sbjct: 181 KSEISVLESSINETQKVHR--DQTKKKIIQTSYSDQFLPTKII----------------- 240

Query: 241 QLDVFQVEERGFYLRMVCKMGEGVAMSLYKVLESLTSFNIQSSNLASASDRFILTATINV 300
           QLDVFQVEERGFYLR+VCKMGE VAMSLYKVLESLTSF IQSSNL SASDRFILTATINV
Sbjct: 241 QLDVFQVEERGFYLRLVCKMGERVAMSLYKVLESLTSFIIQSSNLTSASDRFILTATINV 300

Query: 301 RDCEVDMNLPNMKLWLTGALLNHGFEF 304
           RDCEVDMNLPN+KLWLTGALLNHGFEF
Sbjct: 301 RDCEVDMNLPNLKLWLTGALLNHGFEF 307

BLAST of MS005114 vs. ExPASy Swiss-Prot
Match: Q0V7X4 (Transcription factor FER-LIKE IRON DEFICIENCY-INDUCED TRANSCRIPTION FACTOR OS=Arabidopsis thaliana OX=3702 GN=FIT PE=1 SV=1)

HSP 1 Score: 251.9 bits (642), Expect = 9.1e-66
Identity = 165/325 (50.77%), Postives = 207/325 (63.69%), Query Frame = 0

Query: 9   MMNINDFELQDFIHGDNFDQYVNLIRGE----PENPTFNFDL------EYMLDSNIVMNS 68
           + NIND EL +F+   NFDQ++NLIRG+     ENP  +FDL         +D N  + +
Sbjct: 8   LSNINDLELHNFLVDPNFDQFINLIRGDHQTIDENPVLDFDLGPLQNSPCFIDENQFIPT 67

Query: 69  DPNSLFGSL--------ESF-----NSIMREVEDEDEDDTSVENSSITTT----TKKPKV 128
             + LF  L        ESF     +S+    E+++ED    ++SS TTT    T+K K 
Sbjct: 68  PVDDLFDELPDLDSNVAESFRSFDGDSVRAGGEEDEEDYNDGDDSSATTTNNDGTRKTKT 127

Query: 129 DRSRTLISERRRRGRMKEKLYALRSLVPNITKMDKASIVGDAVLYVKELQMQAKKLKAEI 188
           DRSRTLISERRRRGRMK+KLYALRSLVPNITKMDKASIVGDAVLYV+ELQ QAKKLK++I
Sbjct: 128 DRSRTLISERRRRGRMKDKLYALRSLVPNITKMDKASIVGDAVLYVQELQSQAKKLKSDI 187

Query: 189 SVLESSINEI--QTHHRPTDQSHRKIQTSRSDQFLPNKIIQAMMFCFFQLLIWNLGFQLD 248
           + LE+S+N       H P  Q   K Q  R      +K I                 Q+D
Sbjct: 188 AGLEASLNSTGGYQEHAPDAQ---KTQPFRGINPPASKKI----------------IQMD 247

Query: 249 VFQVEERGFYLRMVCKMGEGVAMSLYKVLESLTSFNIQSSNLASAS-DRFILTATINVRD 304
           V QVEE+GFY+R+VC  GEGVA SLYK LESLTSF +Q+SNL+S S D ++LT T++   
Sbjct: 248 VIQVEEKGFYVRLVCNKGEGVAPSLYKSLESLTSFQVQNSNLSSPSPDTYLLTYTLDGTC 307

BLAST of MS005114 vs. ExPASy Swiss-Prot
Match: A0A0N7KIY3 (Transcription factor BHLH156 OS=Oryza sativa subsp. japonica OX=39947 GN=BHLH156 PE=1 SV=1)

HSP 1 Score: 113.2 bits (282), Expect = 5.1e-24
Identity = 83/226 (36.73%), Postives = 122/226 (53.98%), Query Frame = 0

Query: 94  TTTKKPKVDRSRTLISERRRRGRMKEKLYALRSLVPNITKMDKASIVGDAVLYVKELQMQ 153
           + T+K + DRS+T++SER+RR RMKEKLY LR+LVPNITKMDKASI+ DAV+YVK+LQ  
Sbjct: 124 SATRKRR-DRSKTIVSERKRRVRMKEKLYELRALVPNITKMDKASIIADAVVYVKDLQAH 183

Query: 154 AKKLKAEISVLESSINEIQTHHRPTDQSHRKIQTSRSDQFLPNKIIQAMMFCFFQLLIWN 213
           A+KLK E++ LE +        RP   S    +  R  + +     Q         +   
Sbjct: 184 ARKLKEEVAALEEA-----RPIRPPPPSAAAQRPQRQPRRVAAAAAQLARAADAAAVTTA 243

Query: 214 LG-------FQLDVFQVEERGFYLRMVCKM--------GEGVAMSLYKVLESLTSFNIQS 273
                      +   QV E  F++ + C+         G GVA  +   +ESL+ F ++S
Sbjct: 244 AAAPHGARVAHVGAAQVGEGRFFVTVECEPAAAAARGGGGGVAAPVCAAVESLSCFTVES 303

Query: 274 SNLASASDRFILTATINVRDCEVDMNLPN---MKLWLTGALLNHGF 302
           S +  + DR + T T+ V + E D++  +   +KLW+  ALL  GF
Sbjct: 304 STVGCSPDRVVATLTLKVSEAEEDVSAISECTVKLWVMAALLKEGF 343

BLAST of MS005114 vs. ExPASy Swiss-Prot
Match: Q9ZVX2 (Transcription factor ABORTED MICROSPORES OS=Arabidopsis thaliana OX=3702 GN=AMS PE=1 SV=2)

HSP 1 Score: 80.1 bits (196), Expect = 4.7e-14
Identity = 65/216 (30.09%), Postives = 111/216 (51.39%), Query Frame = 0

Query: 49  MLDSNIVMNSDPNSLFGSLESFNSIMREVEDEDEDDTSVENSSITTTTKKPKVDRSRTLI 108
           +++   V+N    +  G ++S +    +++DED+              K  K  +++ L+
Sbjct: 267 LVNEQQVVNDKDMNENGRVDSGSDCSDQIDDEDDPKYK---------KKSGKGSQAKNLM 326

Query: 109 SERRRRGRMKEKLYALRSLVPNITKMDKASIVGDAVLYVKELQMQAKKLKAEI---SVLE 168
           +ERRRR ++ ++LYALRSLVP ITK+D+ASI+GDA+ YVKELQ +AK+L+ E+   S  E
Sbjct: 327 AERRRRKKLNDRLYALRSLVPRITKLDRASILGDAINYVKELQNEAKELQDELEENSETE 386

Query: 169 SSINEIQ----------THHRPTDQSHRKIQTSRSDQFLPNKIIQAMMFCFFQLLIWNLG 228
              N  Q          T   P    +  + + + D  L N   +             + 
Sbjct: 387 DGSNRPQGGMSLNGTVVTGFHPGLSCNSNVPSVKQDVDLENSNDKGQ----------EME 446

Query: 229 FQLDVFQVEERGFYLRMVCKMGEGVAMSLYKVLESL 252
            Q+DV Q++ R F+++++C+   G    L + L+SL
Sbjct: 447 PQVDVAQLDGREFFVKVICEYKPGGFTRLMEALDSL 463

BLAST of MS005114 vs. ExPASy Swiss-Prot
Match: Q2HIV9 (Transcription factor bHLH35 OS=Arabidopsis thaliana OX=3702 GN=BHLH35 PE=2 SV=1)

HSP 1 Score: 74.7 bits (182), Expect = 2.0e-12
Identity = 65/204 (31.86%), Postives = 104/204 (50.98%), Query Frame = 0

Query: 80  EDEDDTSVENSSITTTTKKPKVDRSRTLISERRRRGRMKEKLYALRSLVPNITKMDKASI 139
           E+    S ++SS       P    S+ ++SER RR ++ ++L+ALRS+VPNITKMDKASI
Sbjct: 33  EEAISGSYDSSSPDGAASSP---ASKNIVSERNRRQKLNQRLFALRSVVPNITKMDKASI 92

Query: 140 VGDAVLYVKELQMQAKKLKAEISVLESSINEIQTHHRPTDQSHRKIQTSRSDQFLPNKII 199
           + DA+ Y++ LQ + KKL+AEI  LES+     +  +  D+      TS+  + L +   
Sbjct: 93  IKDAISYIEGLQYEEKKLEAEIRELESTPKSSLSFSKDFDRDLLVPVTSKKMKQLDSGSS 152

Query: 200 QAMMFCFFQLLIWNLGFQLDVFQVEERGFYLRMVCKMGEGVAMSLYKVLESLTSFNIQSS 259
            +++             +L V  + ER   + + C       + L +V ESL +  I +S
Sbjct: 153 TSLIEV----------LELKVTFMGERTMVVSVTCNKRTDTMVKLCEVFESL-NLKILTS 212

Query: 260 NLASASDRFILTATINVRDCEVDM 284
           NL S S     T  I   + E ++
Sbjct: 213 NLTSFSGMIFHTVFIEADEEEQEV 222

BLAST of MS005114 vs. ExPASy Swiss-Prot
Match: Q0JEB7 (Transcription factor BHLH6 OS=Oryza sativa subsp. japonica OX=39947 GN=BHLH6 PE=1 SV=1)

HSP 1 Score: 68.9 bits (167), Expect = 1.1e-10
Identity = 33/64 (51.56%), Postives = 51/64 (79.69%), Query Frame = 0

Query: 104 SRTLISERRRRGRMKEKLYALRSLVPNITKMDKASIVGDAVLYVKELQMQAKKLKAEISV 163
           ++ ++ ER RR ++ EKLYALRS+VPNITKMDKASI+ DA+ Y++ LQ + +++  E++ 
Sbjct: 92  NKNILMERDRRRKLNEKLYALRSVVPNITKMDKASIIKDAIEYIQRLQAEEQQMLREVAA 151

Query: 164 LESS 168
           LES+
Sbjct: 152 LESA 155

BLAST of MS005114 vs. ExPASy TrEMBL
Match: A0A6J1CGE1 (transcription factor FER-LIKE IRON DEFICIENCY-INDUCED TRANSCRIPTION FACTOR OS=Momordica charantia OX=3673 GN=LOC111011295 PE=4 SV=1)

HSP 1 Score: 542.3 bits (1396), Expect = 1.2e-150
Identity = 286/303 (94.39%), Postives = 286/303 (94.39%), Query Frame = 0

Query: 1   MEPIGKPLMMNINDFELQDFIHGDNFDQYVNLIRGEPENPTFNFDLEYMLDSNIVMNSDP 60
           MEPIGKPLMMNINDFELQDFIHGDNFDQYVNLIRGEPENPTFNFDLEYMLDSNIVMNSDP
Sbjct: 4   MEPIGKPLMMNINDFELQDFIHGDNFDQYVNLIRGEPENPTFNFDLEYMLDSNIVMNSDP 63

Query: 61  NSLFGSLESFNSIMREVEDEDEDDTSVENSSITTTTKKPKVDRSRTLISERRRRGRMKEK 120
           NSLFGSLESFNSIMREVEDEDEDDTSVENSSITTTTKKPKVDRSRTLISERRRRGRMKEK
Sbjct: 64  NSLFGSLESFNSIMREVEDEDEDDTSVENSSITTTTKKPKVDRSRTLISERRRRGRMKEK 123

Query: 121 LYALRSLVPNITKMDKASIVGDAVLYVKELQMQAKKLKAEISVLESSINEIQTHHRPTDQ 180
           LYALRSLVPNITKMDKASIVGDAVLYVKELQMQAKKLKAEISVLESSINEIQTHHRPTDQ
Sbjct: 124 LYALRSLVPNITKMDKASIVGDAVLYVKELQMQAKKLKAEISVLESSINEIQTHHRPTDQ 183

Query: 181 SHRKIQTSRSDQFLPNKIIQAMMFCFFQLLIWNLGFQLDVFQVEERGFYLRMVCKMGEGV 240
           SHRKIQTSRSDQFLPNKII                 QLDVFQVEERGFYLRMVCKMGEGV
Sbjct: 184 SHRKIQTSRSDQFLPNKII-----------------QLDVFQVEERGFYLRMVCKMGEGV 243

Query: 241 AMSLYKVLESLTSFNIQSSNLASASDRFILTATINVRDCEVDMNLPNMKLWLTGALLNHG 300
           AMSLYKVLESLTSFNIQSSNLASASDRFILTATINVRDCEVDMNLPNMKLWLTGALLNHG
Sbjct: 244 AMSLYKVLESLTSFNIQSSNLASASDRFILTATINVRDCEVDMNLPNMKLWLTGALLNHG 289

Query: 301 FEF 304
           FEF
Sbjct: 304 FEF 289

BLAST of MS005114 vs. ExPASy TrEMBL
Match: A0A6J1FTZ6 (transcription factor FER-LIKE IRON DEFICIENCY-INDUCED TRANSCRIPTION FACTOR-like OS=Cucurbita moschata OX=3662 GN=LOC111448427 PE=4 SV=1)

HSP 1 Score: 443.0 bits (1138), Expect = 1.0e-120
Identity = 254/320 (79.38%), Postives = 268/320 (83.75%), Query Frame = 0

Query: 1   MEPIGKPLMMNINDFELQDFIHGDNFDQYVNLIRGEPENPTF-NFDL------------- 60
           ME +GK LMMNIN+FELQDFIHGDNFDQYVNLIRG  ENP F N DL             
Sbjct: 1   MEHVGKHLMMNINEFELQDFIHGDNFDQYVNLIRGGSENPIFDNLDLDFMNVDNRLDPSA 60

Query: 61  EYMLDSNIVMNSDPNSLFGSLESFNSIMREV--EDEDEDDTSVENSSITTTTKKPKVDRS 120
           EY+LDSNIVMNSDPNSLF SLESFNSIM+EV  EDEDEDDTSVENSS+ TT+KK KVDRS
Sbjct: 61  EYILDSNIVMNSDPNSLFSSLESFNSIMKEVENEDEDEDDTSVENSSV-TTSKKSKVDRS 120

Query: 121 RTLISERRRRGRMKEKLYALRSLVPNITKMDKASIVGDAVLYVKELQMQAKKLKAEISVL 180
           RTLISERRRRGRMKEKLYALRSLVPNITKMDKASIVGDAVLYVKELQ+QAKKLKAEISVL
Sbjct: 121 RTLISERRRRGRMKEKLYALRSLVPNITKMDKASIVGDAVLYVKELQVQAKKLKAEISVL 180

Query: 181 ESSINEIQTHHRPTDQSHRKI-QTSRSDQFLPNKIIQAMMFCFFQLLIWNLGFQLDVFQV 240
           ESSINE QT HR   Q+H+KI QTS S QFLP KII                 QLDVFQV
Sbjct: 181 ESSINETQTSHR--GQNHKKIVQTSHSGQFLPCKII-----------------QLDVFQV 240

Query: 241 EERGFYLRMVCKMGEGVAMSLYKVLESLTSFNIQSSNLASASDRFILTATINVRDCEVDM 300
           EERGFYLR+VCKMGE VAMSLYKVLESLTSF+IQSSNLASASDRFILTATINVR+CE+DM
Sbjct: 241 EERGFYLRLVCKMGERVAMSLYKVLESLTSFSIQSSNLASASDRFILTATINVRNCEMDM 300

Query: 301 NLPNMKLWLTGALLNHGFEF 304
           NLPN+KLWLTGALLNHGFEF
Sbjct: 301 NLPNLKLWLTGALLNHGFEF 300

BLAST of MS005114 vs. ExPASy TrEMBL
Match: A0A6J1J7B0 (transcription factor FER-LIKE IRON DEFICIENCY-INDUCED TRANSCRIPTION FACTOR-like OS=Cucurbita maxima OX=3661 GN=LOC111484117 PE=4 SV=1)

HSP 1 Score: 434.5 bits (1116), Expect = 3.7e-118
Identity = 250/320 (78.12%), Postives = 265/320 (82.81%), Query Frame = 0

Query: 1   MEPIGKPLMMNINDFELQDFIHGDNFDQYVNLIRGEPENPTF-NFDL------------- 60
           ME +GK LMMNIN+FELQD IHGDNFDQYVNLIRG  ENP F N DL             
Sbjct: 1   MEHVGKHLMMNINEFELQDLIHGDNFDQYVNLIRGGSENPIFDNLDLDFMNVDNRLDPSA 60

Query: 61  EYMLDSNIVMNSDPNSLFGSLESFNSIMREV--EDEDEDDTSVENSSITTTTKKPKVDRS 120
           EY+LDSNIVMNSDPNSLF SLESFNSIM+EV  EDEDEDD SVENSS+ TT+KK KVDRS
Sbjct: 61  EYILDSNIVMNSDPNSLFSSLESFNSIMKEVETEDEDEDDASVENSSV-TTSKKSKVDRS 120

Query: 121 RTLISERRRRGRMKEKLYALRSLVPNITKMDKASIVGDAVLYVKELQMQAKKLKAEISVL 180
           RTLISERRRRGRMKEKLYALRSLVPNITKMDKASIVGDAVLYVKELQ+QAKKLKAEISVL
Sbjct: 121 RTLISERRRRGRMKEKLYALRSLVPNITKMDKASIVGDAVLYVKELQVQAKKLKAEISVL 180

Query: 181 ESSINEIQTHHRPTDQSHRKI-QTSRSDQFLPNKIIQAMMFCFFQLLIWNLGFQLDVFQV 240
           ESSINE QT +R   Q+H+ I QTS S QFLP KII                 QLDVFQV
Sbjct: 181 ESSINETQTSYR--GQNHKNIVQTSHSGQFLPCKII-----------------QLDVFQV 240

Query: 241 EERGFYLRMVCKMGEGVAMSLYKVLESLTSFNIQSSNLASASDRFILTATINVRDCEVDM 300
           EERGFYLR+VCKMGE VAMSLYKVLESLTSF+IQSSNLASASDRFILTATINVR+CE+DM
Sbjct: 241 EERGFYLRLVCKMGERVAMSLYKVLESLTSFSIQSSNLASASDRFILTATINVRNCEMDM 300

Query: 301 NLPNMKLWLTGALLNHGFEF 304
           NLPN+KLWLTGALLNHGFEF
Sbjct: 301 NLPNLKLWLTGALLNHGFEF 300

BLAST of MS005114 vs. ExPASy TrEMBL
Match: A0A5A7T764 (Transcription factor FER-LIKE IRON DEFICIENCY-INDUCED TRANSCRIPTION FACTOR isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold3G00170 PE=4 SV=1)

HSP 1 Score: 420.6 bits (1080), Expect = 5.5e-114
Identity = 243/328 (74.09%), Postives = 262/328 (79.88%), Query Frame = 0

Query: 1   MEPIGKPLMMNIND-FELQDFIHGDNFDQYVNLIRGEPENPTF--NFD------------ 60
           ME  G+ LM +IND FELQDFIHGDNFD+YVNLIRG  E P F  NFD            
Sbjct: 1   MEQFGRHLMTDINDQFELQDFIHGDNFDRYVNLIRGGNETPIFNNNFDLDFVNGCLIEDR 60

Query: 61  -----LEYMLDSNIVMNSDPNSLFGSLESFNSIMREVEDEDEDDTSVENSSI----TTTT 120
                LEY++DSN+VMNSDPNSLF +LESFNSIM+EVE+EDED+   EN S+    +TT+
Sbjct: 61  VVDQSLEYIVDSNMVMNSDPNSLFSTLESFNSIMKEVENEDEDEDEDENESVENSSSTTS 120

Query: 121 KKPKVDRSRTLISERRRRGRMKEKLYALRSLVPNITKMDKASIVGDAVLYVKELQMQAKK 180
           KKPK DR+RTLISERRRRGRMKEKLYALRSLVPNITKMDKASIVGDAVLYVKELQMQAKK
Sbjct: 121 KKPKADRTRTLISERRRRGRMKEKLYALRSLVPNITKMDKASIVGDAVLYVKELQMQAKK 180

Query: 181 LKAEISVLESSINEIQTHHRPTDQSHRK-IQTSRSDQFLPNKIIQAMMFCFFQLLIWNLG 240
           LKAEISVLESSINE Q  HR  DQ+ +K IQTS SDQFLP KII                
Sbjct: 181 LKAEISVLESSINETQKIHR--DQTKKKIIQTSYSDQFLPTKII---------------- 240

Query: 241 FQLDVFQVEERGFYLRMVCKMGEGVAMSLYKVLESLTSFNIQSSNLASASDRFILTATIN 300
            QLDVFQVEERGFYLR+VCKMGE VAMSLYKVLESLT+F IQSSNLASASDRFILTATIN
Sbjct: 241 -QLDVFQVEERGFYLRLVCKMGERVAMSLYKVLESLTNFIIQSSNLASASDRFILTATIN 300

Query: 301 VRDCEVDMNLPNMKLWLTGALLNHGFEF 304
           VRDCEVDMNLPN+KLWLTGALLNHGFEF
Sbjct: 301 VRDCEVDMNLPNLKLWLTGALLNHGFEF 309

BLAST of MS005114 vs. ExPASy TrEMBL
Match: A0A1S3CQB6 (transcription factor FER-LIKE IRON DEFICIENCY-INDUCED TRANSCRIPTION FACTOR isoform X1 OS=Cucumis melo OX=3656 GN=LOC103503037 PE=4 SV=1)

HSP 1 Score: 420.6 bits (1080), Expect = 5.5e-114
Identity = 243/328 (74.09%), Postives = 262/328 (79.88%), Query Frame = 0

Query: 1   MEPIGKPLMMNIND-FELQDFIHGDNFDQYVNLIRGEPENPTF--NFD------------ 60
           ME  G+ LM +IND FELQDFIHGDNFD+YVNLIRG  E P F  NFD            
Sbjct: 1   MEQFGRHLMTDINDQFELQDFIHGDNFDRYVNLIRGGNETPIFNNNFDLDFVNGCLIEDR 60

Query: 61  -----LEYMLDSNIVMNSDPNSLFGSLESFNSIMREVEDEDEDDTSVENSSI----TTTT 120
                LEY++DSN+VMNSDPNSLF +LESFNSIM+EVE+EDED+   EN S+    +TT+
Sbjct: 61  VVDQSLEYIVDSNMVMNSDPNSLFSTLESFNSIMKEVENEDEDEDEDENESVENSSSTTS 120

Query: 121 KKPKVDRSRTLISERRRRGRMKEKLYALRSLVPNITKMDKASIVGDAVLYVKELQMQAKK 180
           KKPK DR+RTLISERRRRGRMKEKLYALRSLVPNITKMDKASIVGDAVLYVKELQMQAKK
Sbjct: 121 KKPKADRTRTLISERRRRGRMKEKLYALRSLVPNITKMDKASIVGDAVLYVKELQMQAKK 180

Query: 181 LKAEISVLESSINEIQTHHRPTDQSHRK-IQTSRSDQFLPNKIIQAMMFCFFQLLIWNLG 240
           LKAEISVLESSINE Q  HR  DQ+ +K IQTS SDQFLP KII                
Sbjct: 181 LKAEISVLESSINETQKIHR--DQTKKKIIQTSYSDQFLPTKII---------------- 240

Query: 241 FQLDVFQVEERGFYLRMVCKMGEGVAMSLYKVLESLTSFNIQSSNLASASDRFILTATIN 300
            QLDVFQVEERGFYLR+VCKMGE VAMSLYKVLESLT+F IQSSNLASASDRFILTATIN
Sbjct: 241 -QLDVFQVEERGFYLRLVCKMGERVAMSLYKVLESLTNFIIQSSNLASASDRFILTATIN 300

Query: 301 VRDCEVDMNLPNMKLWLTGALLNHGFEF 304
           VRDCEVDMNLPN+KLWLTGALLNHGFEF
Sbjct: 301 VRDCEVDMNLPNLKLWLTGALLNHGFEF 309

BLAST of MS005114 vs. TAIR 10
Match: AT2G28160.1 (FER-like regulator of iron uptake )

HSP 1 Score: 251.9 bits (642), Expect = 6.5e-67
Identity = 165/325 (50.77%), Postives = 207/325 (63.69%), Query Frame = 0

Query: 9   MMNINDFELQDFIHGDNFDQYVNLIRGE----PENPTFNFDL------EYMLDSNIVMNS 68
           + NIND EL +F+   NFDQ++NLIRG+     ENP  +FDL         +D N  + +
Sbjct: 8   LSNINDLELHNFLVDPNFDQFINLIRGDHQTIDENPVLDFDLGPLQNSPCFIDENQFIPT 67

Query: 69  DPNSLFGSL--------ESF-----NSIMREVEDEDEDDTSVENSSITTT----TKKPKV 128
             + LF  L        ESF     +S+    E+++ED    ++SS TTT    T+K K 
Sbjct: 68  PVDDLFDELPDLDSNVAESFRSFDGDSVRAGGEEDEEDYNDGDDSSATTTNNDGTRKTKT 127

Query: 129 DRSRTLISERRRRGRMKEKLYALRSLVPNITKMDKASIVGDAVLYVKELQMQAKKLKAEI 188
           DRSRTLISERRRRGRMK+KLYALRSLVPNITKMDKASIVGDAVLYV+ELQ QAKKLK++I
Sbjct: 128 DRSRTLISERRRRGRMKDKLYALRSLVPNITKMDKASIVGDAVLYVQELQSQAKKLKSDI 187

Query: 189 SVLESSINEI--QTHHRPTDQSHRKIQTSRSDQFLPNKIIQAMMFCFFQLLIWNLGFQLD 248
           + LE+S+N       H P  Q   K Q  R      +K I                 Q+D
Sbjct: 188 AGLEASLNSTGGYQEHAPDAQ---KTQPFRGINPPASKKI----------------IQMD 247

Query: 249 VFQVEERGFYLRMVCKMGEGVAMSLYKVLESLTSFNIQSSNLASAS-DRFILTATINVRD 304
           V QVEE+GFY+R+VC  GEGVA SLYK LESLTSF +Q+SNL+S S D ++LT T++   
Sbjct: 248 VIQVEEKGFYVRLVCNKGEGVAPSLYKSLESLTSFQVQNSNLSSPSPDTYLLTYTLDGTC 307

BLAST of MS005114 vs. TAIR 10
Match: AT2G16910.1 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 80.1 bits (196), Expect = 3.4e-15
Identity = 65/216 (30.09%), Postives = 111/216 (51.39%), Query Frame = 0

Query: 49  MLDSNIVMNSDPNSLFGSLESFNSIMREVEDEDEDDTSVENSSITTTTKKPKVDRSRTLI 108
           +++   V+N    +  G ++S +    +++DED+              K  K  +++ L+
Sbjct: 267 LVNEQQVVNDKDMNENGRVDSGSDCSDQIDDEDDPKYK---------KKSGKGSQAKNLM 326

Query: 109 SERRRRGRMKEKLYALRSLVPNITKMDKASIVGDAVLYVKELQMQAKKLKAEI---SVLE 168
           +ERRRR ++ ++LYALRSLVP ITK+D+ASI+GDA+ YVKELQ +AK+L+ E+   S  E
Sbjct: 327 AERRRRKKLNDRLYALRSLVPRITKLDRASILGDAINYVKELQNEAKELQDELEENSETE 386

Query: 169 SSINEIQ----------THHRPTDQSHRKIQTSRSDQFLPNKIIQAMMFCFFQLLIWNLG 228
              N  Q          T   P    +  + + + D  L N   +             + 
Sbjct: 387 DGSNRPQGGMSLNGTVVTGFHPGLSCNSNVPSVKQDVDLENSNDKGQ----------EME 446

Query: 229 FQLDVFQVEERGFYLRMVCKMGEGVAMSLYKVLESL 252
            Q+DV Q++ R F+++++C+   G    L + L+SL
Sbjct: 447 PQVDVAQLDGREFFVKVICEYKPGGFTRLMEALDSL 463

BLAST of MS005114 vs. TAIR 10
Match: AT5G57150.4 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 75.9 bits (185), Expect = 6.4e-14
Identity = 65/198 (32.83%), Postives = 102/198 (51.52%), Query Frame = 0

Query: 80  EDEDDTSVENSSITTTTKKPKVDRSRTLISERRRRGRMKEKLYALRSLVPNITKMDKASI 139
           E+    S ++SS       P    S+ ++SER RR ++ ++L+ALRS+VPNITKMDKASI
Sbjct: 33  EEAISGSYDSSSPDGAASSP---ASKNIVSERNRRQKLNQRLFALRSVVPNITKMDKASI 92

Query: 140 VGDAVLYVKELQMQAKKLKAEISVLESSINEIQTHHRPTDQSHRKIQTSRSDQFLPNKII 199
           + DA+ Y++ LQ + KKL+AEI  LES+     +  +  D+      TS+  + L +   
Sbjct: 93  IKDAISYIEGLQYEEKKLEAEIRELESTPKSSLSFSKDFDRDLLVPVTSKKMKQLDSGSS 152

Query: 200 QAMMFCFFQLLIWNLGFQLDVFQVEERGFYLRMVCKMGEGVAMSLYKVLESLTSFNIQSS 259
            +++             +L V  + ER   + + C       + L +V ESL +  I +S
Sbjct: 153 TSLIEV----------LELKVTFMGERTMVVSVTCNKRTDTMVKLCEVFESL-NLKILTS 212

Query: 260 NLASASDRFILTATINVR 278
           NL S S     T  I +R
Sbjct: 213 NLTSFSGMIFHTVFIELR 216

BLAST of MS005114 vs. TAIR 10
Match: AT5G57150.2 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 75.1 bits (183), Expect = 1.1e-13
Identity = 65/197 (32.99%), Postives = 101/197 (51.27%), Query Frame = 0

Query: 80  EDEDDTSVENSSITTTTKKPKVDRSRTLISERRRRGRMKEKLYALRSLVPNITKMDKASI 139
           E+    S ++SS       P    S+ ++SER RR ++ ++L+ALRS+VPNITKMDKASI
Sbjct: 33  EEAISGSYDSSSPDGAASSP---ASKNIVSERNRRQKLNQRLFALRSVVPNITKMDKASI 92

Query: 140 VGDAVLYVKELQMQAKKLKAEISVLESSINEIQTHHRPTDQSHRKIQTSRSDQFLPNKII 199
           + DA+ Y++ LQ + KKL+AEI  LES+     +  +  D+      TS+  + L +   
Sbjct: 93  IKDAISYIEGLQYEEKKLEAEIRELESTPKSSLSFSKDFDRDLLVPVTSKKMKQLDSGSS 152

Query: 200 QAMMFCFFQLLIWNLGFQLDVFQVEERGFYLRMVCKMGEGVAMSLYKVLESLTSFNIQSS 259
            +++             +L V  + ER   + + C       + L +V ESL +  I +S
Sbjct: 153 TSLIEV----------LELKVTFMGERTMVVSVTCNKRTDTMVKLCEVFESL-NLKILTS 212

Query: 260 NLASASDRFILTATINV 277
           NL S S     T  I V
Sbjct: 213 NLTSFSGMIFHTVFIEV 215

BLAST of MS005114 vs. TAIR 10
Match: AT5G57150.1 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 74.7 bits (182), Expect = 1.4e-13
Identity = 65/204 (31.86%), Postives = 104/204 (50.98%), Query Frame = 0

Query: 80  EDEDDTSVENSSITTTTKKPKVDRSRTLISERRRRGRMKEKLYALRSLVPNITKMDKASI 139
           E+    S ++SS       P    S+ ++SER RR ++ ++L+ALRS+VPNITKMDKASI
Sbjct: 32  EEAISGSYDSSSPDGAASSP---ASKNIVSERNRRQKLNQRLFALRSVVPNITKMDKASI 91

Query: 140 VGDAVLYVKELQMQAKKLKAEISVLESSINEIQTHHRPTDQSHRKIQTSRSDQFLPNKII 199
           + DA+ Y++ LQ + KKL+AEI  LES+     +  +  D+      TS+  + L +   
Sbjct: 92  IKDAISYIEGLQYEEKKLEAEIRELESTPKSSLSFSKDFDRDLLVPVTSKKMKQLDSGSS 151

Query: 200 QAMMFCFFQLLIWNLGFQLDVFQVEERGFYLRMVCKMGEGVAMSLYKVLESLTSFNIQSS 259
            +++             +L V  + ER   + + C       + L +V ESL +  I +S
Sbjct: 152 TSLIEV----------LELKVTFMGERTMVVSVTCNKRTDTMVKLCEVFESL-NLKILTS 211

Query: 260 NLASASDRFILTATINVRDCEVDM 284
           NL S S     T  I   + E ++
Sbjct: 212 NLTSFSGMIFHTVFIEADEEEQEV 221

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022140694.12.6e-15094.39transcription factor FER-LIKE IRON DEFICIENCY-INDUCED TRANSCRIPTION FACTOR [Momo... [more]
XP_022943777.12.1e-12079.38transcription factor FER-LIKE IRON DEFICIENCY-INDUCED TRANSCRIPTION FACTOR-like ... [more]
XP_022986352.17.6e-11878.13transcription factor FER-LIKE IRON DEFICIENCY-INDUCED TRANSCRIPTION FACTOR-like ... [more]
XP_038902250.12.3e-11475.69transcription factor FER-LIKE IRON DEFICIENCY-INDUCED TRANSCRIPTION FACTOR [Beni... [more]
XP_004145819.13.0e-11475.54transcription factor FER-LIKE IRON DEFICIENCY-INDUCED TRANSCRIPTION FACTOR [Cucu... [more]
Match NameE-valueIdentityDescription
Q0V7X49.1e-6650.77Transcription factor FER-LIKE IRON DEFICIENCY-INDUCED TRANSCRIPTION FACTOR OS=Ar... [more]
A0A0N7KIY35.1e-2436.73Transcription factor BHLH156 OS=Oryza sativa subsp. japonica OX=39947 GN=BHLH156... [more]
Q9ZVX24.7e-1430.09Transcription factor ABORTED MICROSPORES OS=Arabidopsis thaliana OX=3702 GN=AMS ... [more]
Q2HIV92.0e-1231.86Transcription factor bHLH35 OS=Arabidopsis thaliana OX=3702 GN=BHLH35 PE=2 SV=1[more]
Q0JEB71.1e-1051.56Transcription factor BHLH6 OS=Oryza sativa subsp. japonica OX=39947 GN=BHLH6 PE=... [more]
Match NameE-valueIdentityDescription
A0A6J1CGE11.2e-15094.39transcription factor FER-LIKE IRON DEFICIENCY-INDUCED TRANSCRIPTION FACTOR OS=Mo... [more]
A0A6J1FTZ61.0e-12079.38transcription factor FER-LIKE IRON DEFICIENCY-INDUCED TRANSCRIPTION FACTOR-like ... [more]
A0A6J1J7B03.7e-11878.13transcription factor FER-LIKE IRON DEFICIENCY-INDUCED TRANSCRIPTION FACTOR-like ... [more]
A0A5A7T7645.5e-11474.09Transcription factor FER-LIKE IRON DEFICIENCY-INDUCED TRANSCRIPTION FACTOR isofo... [more]
A0A1S3CQB65.5e-11474.09transcription factor FER-LIKE IRON DEFICIENCY-INDUCED TRANSCRIPTION FACTOR isofo... [more]
Match NameE-valueIdentityDescription
AT2G28160.16.5e-6750.77FER-like regulator of iron uptake [more]
AT2G16910.13.4e-1530.09basic helix-loop-helix (bHLH) DNA-binding superfamily protein [more]
AT5G57150.46.4e-1432.83basic helix-loop-helix (bHLH) DNA-binding superfamily protein [more]
AT5G57150.21.1e-1332.99basic helix-loop-helix (bHLH) DNA-binding superfamily protein [more]
AT5G57150.11.4e-1331.86basic helix-loop-helix (bHLH) DNA-binding superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 147..174
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 77..105
NoneNo IPR availablePANTHERPTHR31945:SF17TRANSCRIPTION FACTOR FER-LIKE IRON DEFICIENCY-INDUCED TRANSCRIPTION FACTORcoord: 65..302
NoneNo IPR availablePANTHERPTHR31945TRANSCRIPTION FACTOR SCREAM2-RELATEDcoord: 65..302
IPR011598Myc-type, basic helix-loop-helix (bHLH) domainSMARTSM00353finuluscoord: 107..156
e-value: 6.4E-13
score: 58.9
IPR011598Myc-type, basic helix-loop-helix (bHLH) domainPFAMPF00010HLHcoord: 107..151
e-value: 2.4E-9
score: 37.0
IPR011598Myc-type, basic helix-loop-helix (bHLH) domainPROSITEPS50888BHLHcoord: 101..150
score: 15.237004
IPR036638Helix-loop-helix DNA-binding domain superfamilyGENE3D4.10.280.10coord: 92..181
e-value: 2.2E-15
score: 58.3
IPR036638Helix-loop-helix DNA-binding domain superfamilySUPERFAMILY47459HLH, helix-loop-helix DNA-binding domaincoord: 103..178

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS005114.1MS005114.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0046983 protein dimerization activity