MS005051 (gene) Bitter gourd (TR) v1

Overview


Name	MS005051
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	vicilin-like seed storage protein At2g28490
Location	scaffold176: 2347075 .. 2349210 (+)
RNA-Seq Expression	MS005051
Synteny	MS005051

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGAAAGAGAGAAGCTCTGGTGGTGCTGTTGATCATCGCCGTTTTGGGAAACGCAGTTGGGTTCGACAGAGAAGAAGAAGCAGAGTGGGAGAGAGATAGGAGGGAAGAGAGAGAGTTTAGAAGGGAAGACCGGTTTCTGCTGGAGGATTCGAAGCATGTGATACAGACAGAGGCGGGAGAGATGAGGGTGGTCAAAGGTCCAGCTTCAAGAATTCTCGACAGGCAAATTCACATTGGCTTCATCACTATGGAGCCCAAGTCCCTGTTCATCCCTCAGTATATGGACTCCAGCCTGATTCTCTTCGTCCAAAAAGGTATTCACCCTTATTTCCTCAATCTCTTCAAAACAATTCGCCTCATGCATTCATAAATCACTACACCCAAAAGCACTCTTAGCATTACACCTAACAAGCGATCATCAAAATTAATGTGGAGGCGATGGCATAGACCCAAGAGCACTCTAGCACAAGACCTAACAAGTGATTGTCAACGATAATGTGGAGGAAGTTAGAGTCCACAAAGCTTAAACAGATGGGTTACAATACTAAATTTGATCTGTTATTCACCTTCTTAGAATGCATTTAGTTTTCTGTAGTTACAATGAATGTGAATGGAAGGGGGAAGTTTTCAGCCATTGAAGGGAATGGGAAATGTAAATAATTGCAGGGGAAGTGAGGCTGGGATTGATATACAAAGACGAATTAGCAGAGAGGAGGATGAAAGAAGGAGATGTATACCGGATCCCAGCCGGTTCGGTGTTTTACATGGTGAACATAGGGGAAGGACAGAGACTTCAAATTATCTGCAGCATTGATAGAGCTGGGAGTTGGAGTCCCGGTACTGTCCAGGTAATTAATCTCTTAGTTTGAATTGGTGGTGGGGTTGGAAGGAATGAAATAAGAATCCGAATTTTGAAATGGAGATTTGATTCTTGTTTTTTCTTCTTCTAATTATAGTCCTTCTTCGTCGCTGGAGGAACCGATCCGGAATCAATCCTTGCCGGATTCGACCAGGATACATTGGCTGCAGCATTTAATGTATGAAATTCAACTAAAATTCTTGTTCTGCTTACCTTGAATCTTACGGCAGCATCTGCATTGAACTTTCACACGAATCAATTTTCCCCACAGGTTTCTCGTACAGAACTGAAGAGACTTATGACGAGGCAACGACAGGGTCCAATCGTATACGTCTCGGATACTGAATCGCCTAGGGTTTTGAGTAAGTTTCTGCAAGTGAAAGATGAAGACAGATTAAATACAATTGCCGGTGTCAATGAAGAAAGCGGAGAGGCGGAGAAGAAAAAGTCTTGGTCGTGGAGGAAGCTCCTGGGTTCAATCTTTGGAAACGAGAATCGCGACACACCGAAAACAAAAACCGGAAAATCCCCTGATTCCTACAACCTCCGCGAAAAATCTCCAGATTTCAGCAACGCTTACGGAAAGAGTGTCGCCCTCGACGAGACTGAGTACTCTCCTCTACGTGACTCCAGAGTCGGAGTTTATCTCGTCTATCTCAACGCGGTAAATCGAATCTGTTGTTCAATCCATAATCCTCTCAATTAATTTCTATTACATTCTACATCTGATTCATGGATCAGTTAATTAATTTCAAGAGAGAACTGACCTCCGACCTCCGCTTACAGGGATCGATGATGGCGCCGCACGTGAATCCAGACGCGGACGAGTTTGCCATCGTTACCGTAGGCAAAGGTAAGATGCAGATCACATATCCCAACGGAACATCCGCCTTCGATGCAGAAGTGAGCGAAGGGAACGTGATCTTTTTTCCAAAATTCTTCGCGTTCTGCCAAATCGCATCCAGAACAGGCCCGTTCGAGTTCGTGGGATTCACCACGTCGTCGCACAGGAATCATCCGCAGTTCTTGGCCGGTGCGAGTTCGGTTTACCACACTCTTAGAGGCCCCGAGATGGCAAGGGCCTTCGACATGGCCGAGGACGATCTGGGCCGCTTCCTTGACGCACAGAGCGAGGCGATCATTCTGCCGTCGGCGGAGATTGCGCCGCCGTACAAGGAGGAGGAGAAGGGGAGGCAAGAGGAAGAGAAGCGGGAAAGGAGAGACAGAGAGGCAATAAGGAGCTTAGAAGGGAATATTATGGGGATGGGATTTGAT

mRNA sequence

ATGGGAAAGAGAGAAGCTCTGGTGGTGCTGTTGATCATCGCCGTTTTGGGAAACGCAGTTGGGTTCGACAGAGAAGAAGAAGCAGAGTGGGAGAGAGATAGGAGGGAAGAGAGAGAGTTTAGAAGGGAAGACCGGTTTCTGCTGGAGGATTCGAAGCATGTGATACAGACAGAGGCGGGAGAGATGAGGGTGGTCAAAGGTCCAGCTTCAAGAATTCTCGACAGGCAAATTCACATTGGCTTCATCACTATGGAGCCCAAGTCCCTGTTCATCCCTCAGTATATGGACTCCAGCCTGATTCTCTTCGTCCAAAAAGGGGAAGTGAGGCTGGGATTGATATACAAAGACGAATTAGCAGAGAGGAGGATGAAAGAAGGAGATGTATACCGGATCCCAGCCGGTTCGGTGTTTTACATGGTGAACATAGGGGAAGGACAGAGACTTCAAATTATCTGCAGCATTGATAGAGCTGGGAGTTGGAGTCCCGGTACTGTCCAGTCCTTCTTCGTCGCTGGAGGAACCGATCCGGAATCAATCCTTGCCGGATTCGACCAGGATACATTGGCTGCAGCATTTAATGTTTCTCGTACAGAACTGAAGAGACTTATGACGAGGCAACGACAGGGTCCAATCGTATACGTCTCGGATACTGAATCGCCTAGGGTTTTGAGTAAGTTTCTGCAAGTGAAAGATGAAGACAGATTAAATACAATTGCCGGTGTCAATGAAGAAAGCGGAGAGGCGGAGAAGAAAAAGTCTTGGTCGTGGAGGAAGCTCCTGGGTTCAATCTTTGGAAACGAGAATCGCGACACACCGAAAACAAAAACCGGAAAATCCCCTGATTCCTACAACCTCCGCGAAAAATCTCCAGATTTCAGCAACGCTTACGGAAAGAGTGTCGCCCTCGACGAGACTGAGTACTCTCCTCTACGTGACTCCAGAGTCGGAGTTTATCTCGTCTATCTCAACGCGGGATCGATGATGGCGCCGCACGTGAATCCAGACGCGGACGAGTTTGCCATCGTTACCGTAGGCAAAGGTAAGATGCAGATCACATATCCCAACGGAACATCCGCCTTCGATGCAGAAGTGAGCGAAGGGAACGTGATCTTTTTTCCAAAATTCTTCGCGTTCTGCCAAATCGCATCCAGAACAGGCCCGTTCGAGTTCGTGGGATTCACCACGTCGTCGCACAGGAATCATCCGCAGTTCTTGGCCGGTGCGAGTTCGGTTTACCACACTCTTAGAGGCCCCGAGATGGCAAGGGCCTTCGACATGGCCGAGGACGATCTGGGCCGCTTCCTTGACGCACAGAGCGAGGCGATCATTCTGCCGTCGGCGGAGATTGCGCCGCCGTACAAGGAGGAGGAGAAGGGGAGGCAAGAGGAAGAGAAGCGGGAAAGGAGAGACAGAGAGGCAATAAGGAGCTTAGAAGGGAATATTATGGGGATGGGATTTGAT

Coding sequence (CDS)

ATGGGAAAGAGAGAAGCTCTGGTGGTGCTGTTGATCATCGCCGTTTTGGGAAACGCAGTTGGGTTCGACAGAGAAGAAGAAGCAGAGTGGGAGAGAGATAGGAGGGAAGAGAGAGAGTTTAGAAGGGAAGACCGGTTTCTGCTGGAGGATTCGAAGCATGTGATACAGACAGAGGCGGGAGAGATGAGGGTGGTCAAAGGTCCAGCTTCAAGAATTCTCGACAGGCAAATTCACATTGGCTTCATCACTATGGAGCCCAAGTCCCTGTTCATCCCTCAGTATATGGACTCCAGCCTGATTCTCTTCGTCCAAAAAGGGGAAGTGAGGCTGGGATTGATATACAAAGACGAATTAGCAGAGAGGAGGATGAAAGAAGGAGATGTATACCGGATCCCAGCCGGTTCGGTGTTTTACATGGTGAACATAGGGGAAGGACAGAGACTTCAAATTATCTGCAGCATTGATAGAGCTGGGAGTTGGAGTCCCGGTACTGTCCAGTCCTTCTTCGTCGCTGGAGGAACCGATCCGGAATCAATCCTTGCCGGATTCGACCAGGATACATTGGCTGCAGCATTTAATGTTTCTCGTACAGAACTGAAGAGACTTATGACGAGGCAACGACAGGGTCCAATCGTATACGTCTCGGATACTGAATCGCCTAGGGTTTTGAGTAAGTTTCTGCAAGTGAAAGATGAAGACAGATTAAATACAATTGCCGGTGTCAATGAAGAAAGCGGAGAGGCGGAGAAGAAAAAGTCTTGGTCGTGGAGGAAGCTCCTGGGTTCAATCTTTGGAAACGAGAATCGCGACACACCGAAAACAAAAACCGGAAAATCCCCTGATTCCTACAACCTCCGCGAAAAATCTCCAGATTTCAGCAACGCTTACGGAAAGAGTGTCGCCCTCGACGAGACTGAGTACTCTCCTCTACGTGACTCCAGAGTCGGAGTTTATCTCGTCTATCTCAACGCGGGATCGATGATGGCGCCGCACGTGAATCCAGACGCGGACGAGTTTGCCATCGTTACCGTAGGCAAAGGTAAGATGCAGATCACATATCCCAACGGAACATCCGCCTTCGATGCAGAAGTGAGCGAAGGGAACGTGATCTTTTTTCCAAAATTCTTCGCGTTCTGCCAAATCGCATCCAGAACAGGCCCGTTCGAGTTCGTGGGATTCACCACGTCGTCGCACAGGAATCATCCGCAGTTCTTGGCCGGTGCGAGTTCGGTTTACCACACTCTTAGAGGCCCCGAGATGGCAAGGGCCTTCGACATGGCCGAGGACGATCTGGGCCGCTTCCTTGACGCACAGAGCGAGGCGATCATTCTGCCGTCGGCGGAGATTGCGCCGCCGTACAAGGAGGAGGAGAAGGGGAGGCAAGAGGAAGAGAAGCGGGAAAGGAGAGACAGAGAGGCAATAAGGAGCTTAGAAGGGAATATTATGGGGATGGGATTTGAT

Protein sequence

MGKREALVVLLIIAVLGNAVGFDREEEAEWERDRREEREFRREDRFLLEDSKHVIQTEAGEMRVVKGPASRILDRQIHIGFITMEPKSLFIPQYMDSSLILFVQKGEVRLGLIYKDELAERRMKEGDVYRIPAGSVFYMVNIGEGQRLQIICSIDRAGSWSPGTVQSFFVAGGTDPESILAGFDQDTLAAAFNVSRTELKRLMTRQRQGPIVYVSDTESPRVLSKFLQVKDEDRLNTIAGVNEESGEAEKKKSWSWRKLLGSIFGNENRDTPKTKTGKSPDSYNLREKSPDFSNAYGKSVALDETEYSPLRDSRVGVYLVYLNAGSMMAPHVNPDADEFAIVTVGKGKMQITYPNGTSAFDAEVSEGNVIFFPKFFAFCQIASRTGPFEFVGFTTSSHRNHPQFLAGASSVYHTLRGPEMARAFDMAEDDLGRFLDAQSEAIILPSAEIAPPYKEEEKGRQEEEKRERRDREAIRSLEGNIMGMGFD

Homology

[Close]

BLAST of MS005051 vs. NCBI nr
Match: XP_022140488.1 (vicilin-like seed storage protein At2g28490, partial [Momordica charantia])

HSP 1 Score: 957.2 bits (2473), Expect = 5.4e-275
Identity = 486/487 (99.79%), Postives = 487/487 (100.00%), Query Frame = 0


Query: 1   MGKREALVVLLIIAVLGNAVGFDREEEAEWERDRREEREFRREDRFLLEDSKHVIQTEAG 60
           MGKREALVVLLIIAVLGNA+GFDREEEAEWERDRREEREFRREDRFLLEDSKHVIQTEAG
Sbjct: 31  MGKREALVVLLIIAVLGNAIGFDREEEAEWERDRREEREFRREDRFLLEDSKHVIQTEAG 90

Query: 61  EMRVVKGPASRILDRQIHIGFITMEPKSLFIPQYMDSSLILFVQKGEVRLGLIYKDELAE 120
           EMRVVKGPASRILDRQIHIGFITMEPKSLFIPQYMDSSLILFVQKGEVRLGLIYKDELAE
Sbjct: 91  EMRVVKGPASRILDRQIHIGFITMEPKSLFIPQYMDSSLILFVQKGEVRLGLIYKDELAE 150

Query: 121 RRMKEGDVYRIPAGSVFYMVNIGEGQRLQIICSIDRAGSWSPGTVQSFFVAGGTDPESIL 180
           RRMKEGDVYRIPAGSVFYMVNIGEGQRLQIICSIDRAGSWSPGTVQSFFVAGGTDPESIL
Sbjct: 151 RRMKEGDVYRIPAGSVFYMVNIGEGQRLQIICSIDRAGSWSPGTVQSFFVAGGTDPESIL 210

Query: 181 AGFDQDTLAAAFNVSRTELKRLMTRQRQGPIVYVSDTESPRVLSKFLQVKDEDRLNTIAG 240
           AGFDQDTLAAAFNVSRTELKRLMTRQRQGPIVYVSDTESPRVLSKFLQVKDEDRLNTIAG
Sbjct: 211 AGFDQDTLAAAFNVSRTELKRLMTRQRQGPIVYVSDTESPRVLSKFLQVKDEDRLNTIAG 270

Query: 241 VNEESGEAEKKKSWSWRKLLGSIFGNENRDTPKTKTGKSPDSYNLREKSPDFSNAYGKSV 300
           VNEESGEAEKKKSWSWRKLLGSIFGNENRDTPKTKTGKSPDSYNLREKSPDFSNAYGKSV
Sbjct: 271 VNEESGEAEKKKSWSWRKLLGSIFGNENRDTPKTKTGKSPDSYNLREKSPDFSNAYGKSV 330

Query: 301 ALDETEYSPLRDSRVGVYLVYLNAGSMMAPHVNPDADEFAIVTVGKGKMQITYPNGTSAF 360
           ALDETEYSPLRDSRVGVYLVYLNAGSMMAPHVNPDADEFAIVTVGKGKMQITYPNGTSAF
Sbjct: 331 ALDETEYSPLRDSRVGVYLVYLNAGSMMAPHVNPDADEFAIVTVGKGKMQITYPNGTSAF 390

Query: 361 DAEVSEGNVIFFPKFFAFCQIASRTGPFEFVGFTTSSHRNHPQFLAGASSVYHTLRGPEM 420
           DAEVSEGNVIFFPKFFAFCQIASRTGPFEFVGFTTSSHRNHPQFLAGASSVYHTLRGPEM
Sbjct: 391 DAEVSEGNVIFFPKFFAFCQIASRTGPFEFVGFTTSSHRNHPQFLAGASSVYHTLRGPEM 450

Query: 421 ARAFDMAEDDLGRFLDAQSEAIILPSAEIAPPYKEEEKGRQEEEKRERRDREAIRSLEGN 480
           ARAFDMAEDDLGRFLDAQSEAIILPSAEIAPPYKEEEKGRQEEEKRERRDREAIRSLEGN
Sbjct: 451 ARAFDMAEDDLGRFLDAQSEAIILPSAEIAPPYKEEEKGRQEEEKRERRDREAIRSLEGN 510

Query: 481 IMGMGFD 488
           IMGMGFD
Sbjct: 511 IMGMGFD 517

[Close]

BLAST of MS005051 vs. NCBI nr
Match: XP_023511963.1 (vicilin-like seed storage protein At2g28490 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 688.3 bits (1775), Expect = 4.7e-194
Identity = 351/483 (72.67%), Postives = 405/483 (83.85%), Query Frame = 0


Query: 1   MGKREALVVLLIIAVLGNAVGF----DREEEAEWERDRREEREFRREDRFLLEDSKHVIQ 60
           MGK+EALV+LLIIAVLGNA+G     + EEE EW R+R EEREFR ++RFLLEDSK VI+
Sbjct: 1   MGKKEALVMLLIIAVLGNAIGIKEEAEAEEEEEWWREREEEREFRSKERFLLEDSKRVIE 60

Query: 61  TEAGEMRVVKGPASRILDRQIHIGFITMEPKSLFIPQYMDSSLILFVQKGEVRLGLIYKD 120
           TEAGEMRV++ PASRILDR +HIGFITMEPKSLF+PQY+DSSLILFV++GEV++GLIYKD
Sbjct: 61  TEAGEMRVIRSPASRILDRPMHIGFITMEPKSLFVPQYLDSSLILFVRRGEVKVGLIYKD 120

Query: 121 ELAERRMKEGDVYRIPAGSVFYMVNIGEGQRLQIICSIDRAGSWSPGTVQSFFVAGGTDP 180
           ELAERRMK GDVYRIPAGSVFYMVN+GEGQRLQIICSID++ S S GT QSFF+ GGT P
Sbjct: 121 ELAERRMKGGDVYRIPAGSVFYMVNVGEGQRLQIICSIDKSESLSYGTFQSFFIGGGTYP 180

Query: 181 ESILAGFDQDTLAAAFNVSRTELKRLMTRQRQGPIVYVSDTESPRVLSKFLQVKDEDRLN 240
            S+LAGFDQDTLA AFNVS TEL+R+++RQRQGPIVY+SDTESP V SKFLQVKD D+ N
Sbjct: 181 VSVLAGFDQDTLATAFNVSYTELRRILSRQRQGPIVYISDTESPGVWSKFLQVKDGDKGN 240

Query: 241 TIAGVNEESGEAEKKKSWSWRKLLGSIFGNENRD-TPKTKTGKSPDSYNLREKSPDFSNA 300
            IA +NE+  EAEK K+WSWR L+ SIFGNENRD T +T+TGKSPDSYNL +K+PDFSNA
Sbjct: 241 KIATINEDGEEAEKNKTWSWRNLMSSIFGNENRDKTKRTRTGKSPDSYNLYDKTPDFSNA 300

Query: 301 YGKSVALDETEYSPLRDSRVGVYLVYLNAGSMMAPHVNPDADEFAIVTVGKGKMQITYPN 360
           YG SVALDE EYSPL  S +GVYLV L AGSMMAPH+NP A E+ IV  G G +QI YPN
Sbjct: 301 YGWSVALDEHEYSPLGHSGIGVYLVNLTAGSMMAPHINPTAAEYGIVLRGTGTIQIVYPN 360

Query: 361 GTSAFDAEVSEGNVIFFPKFFAFCQIASRTGPFEFVGFTTSSHRNHPQFLAGASSVYHTL 420
           GTSA D EV+EG+V + P++F FCQIASRTGPFEF GFTTSS RN PQFLAGA+SV+HTL
Sbjct: 361 GTSAMDTEVTEGDVFWVPRYFPFCQIASRTGPFEFFGFTTSSRRNRPQFLAGANSVFHTL 420

Query: 421 RGPEMARAFDMAEDDLGRFLDAQSEAIILPSAEIAPPYKEEEKGRQEEEKRERRDREAIR 479
           R P +A AFD+ EDDL R L  Q E +ILPSAEIAPP+KEEEK R+ EE R  R+RE+ R
Sbjct: 421 RSPAVASAFDITEDDLDRLLSLQHEVVILPSAEIAPPHKEEEKRRRREEGRRERERESER 480

[Close]

BLAST of MS005051 vs. NCBI nr
Match: KAG6570767.1 (Vicilin-like seed storage protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 685.6 bits (1768), Expect = 3.0e-193
Identity = 349/483 (72.26%), Postives = 403/483 (83.44%), Query Frame = 0


Query: 1   MGKREALVVLLIIAVLGNAVGF----DREEEAEWERDRREEREFRREDRFLLEDSKHVIQ 60
           MGK+EALV+LLIIAVLGNA+G     + EEE EW R+R EEREFR ++RFLLEDSK VI+
Sbjct: 1   MGKKEALVMLLIIAVLGNAIGIKEEAEAEEEEEWWREREEEREFRSKERFLLEDSKRVIE 60

Query: 61  TEAGEMRVVKGPASRILDRQIHIGFITMEPKSLFIPQYMDSSLILFVQKGEVRLGLIYKD 120
           TEAGEMRV++ PASRILDR +HIGFITMEPKSLF+PQY+DSSLILFV++GEV++GLIYKD
Sbjct: 61  TEAGEMRVIRSPASRILDRPMHIGFITMEPKSLFVPQYLDSSLILFVRRGEVKVGLIYKD 120

Query: 121 ELAERRMKEGDVYRIPAGSVFYMVNIGEGQRLQIICSIDRAGSWSPGTVQSFFVAGGTDP 180
           ELAERRMK GDVYRIPAGSVFYMVN+GEGQRLQIICS+D++ S S GT QSFFV GGT P
Sbjct: 121 ELAERRMKGGDVYRIPAGSVFYMVNVGEGQRLQIICSVDKSESLSYGTFQSFFVGGGTYP 180

Query: 181 ESILAGFDQDTLAAAFNVSRTELKRLMTRQRQGPIVYVSDTESPRVLSKFLQVKDEDRLN 240
            S+LAGFDQDTLA AFNVS TEL+R+++RQRQGPIVYVSDTESP V SKFLQVKD ++ N
Sbjct: 181 VSVLAGFDQDTLATAFNVSYTELRRILSRQRQGPIVYVSDTESPGVWSKFLQVKDGEKGN 240

Query: 241 TIAGVNEESGEAEKKKSWSWRKLLGSIFGNENRD-TPKTKTGKSPDSYNLREKSPDFSNA 300
            I  +NE+  EAEK K WSWR L+ SIFGNENRD T +T+TGKSPDSYNL +K+PDFSNA
Sbjct: 241 KIPNINEDGEEAEKNKPWSWRNLMSSIFGNENRDKTKRTRTGKSPDSYNLYDKTPDFSNA 300

Query: 301 YGKSVALDETEYSPLRDSRVGVYLVYLNAGSMMAPHVNPDADEFAIVTVGKGKMQITYPN 360
           YG SVALDE EYSPL  S +GVYLV L AGSMMAPH+NP A E+ IV  G G +QI YPN
Sbjct: 301 YGWSVALDEHEYSPLGHSGIGVYLVNLTAGSMMAPHINPTASEYGIVLRGTGTIQIVYPN 360

Query: 361 GTSAFDAEVSEGNVIFFPKFFAFCQIASRTGPFEFVGFTTSSHRNHPQFLAGASSVYHTL 420
           GTSA D EV+EG+V + P++F FCQIASRTGPFEF GFTTSS +N PQFLAGA+S++HTL
Sbjct: 361 GTSAMDTEVTEGDVFWVPRYFPFCQIASRTGPFEFFGFTTSSRKNRPQFLAGANSIFHTL 420

Query: 421 RGPEMARAFDMAEDDLGRFLDAQSEAIILPSAEIAPPYKEEEKGRQEEEKRERRDREAIR 479
           R P +A AFD+ EDDL R L AQ E +ILPSAEIAPP+KEEEK R+ EE R  R+RE  R
Sbjct: 421 RSPAVATAFDITEDDLDRLLSAQYEVVILPSAEIAPPHKEEEKRRRREEGRREREREGER 480

[Close]

BLAST of MS005051 vs. NCBI nr
Match: XP_022985621.1 (vicilin-like seed storage protein At2g28490 [Cucurbita maxima])

HSP 1 Score: 683.3 bits (1762), Expect = 1.5e-192
Identity = 350/483 (72.46%), Postives = 402/483 (83.23%), Query Frame = 0


Query: 1   MGKREALVVLLIIAVLGNAVGFDRE----EEAEWERDRREEREFRREDRFLLEDSKHVIQ 60
           M K+EALV+LLIIAVLGNA+G   E    EE EW R+R EEREFR ++RFLLEDSK VI+
Sbjct: 7   MWKKEALVMLLIIAVLGNAIGIKEEAEAAEEEEWWREREEEREFRSKERFLLEDSKRVIE 66

Query: 61  TEAGEMRVVKGPASRILDRQIHIGFITMEPKSLFIPQYMDSSLILFVQKGEVRLGLIYKD 120
           TEAGEMRV++ PASRILDR +HIGFITMEPKSLF+PQY+DSSLILFV++GEV++GLIYKD
Sbjct: 67  TEAGEMRVIRSPASRILDRPMHIGFITMEPKSLFVPQYLDSSLILFVRRGEVKVGLIYKD 126

Query: 121 ELAERRMKEGDVYRIPAGSVFYMVNIGEGQRLQIICSIDRAGSWSPGTVQSFFVAGGTDP 180
           ELAERRMK GDVYRIPAGSVFYMVN+GEGQRLQIICSID++ S S GT QSFF+ GGT P
Sbjct: 127 ELAERRMKGGDVYRIPAGSVFYMVNVGEGQRLQIICSIDKSESLSYGTFQSFFIGGGTYP 186

Query: 181 ESILAGFDQDTLAAAFNVSRTELKRLMTRQRQGPIVYVSDTESPRVLSKFLQVKDEDRLN 240
            S+LAGFDQDTLA AFNVS TEL+R+++RQRQGPIVYVSDTESP V SKFLQVKD D+ N
Sbjct: 187 VSVLAGFDQDTLATAFNVSYTELRRILSRQRQGPIVYVSDTESPGVWSKFLQVKDGDKGN 246

Query: 241 TIAGVNEESGEAEKKKSWSWRKLLGSIFGNENRD-TPKTKTGKSPDSYNLREKSPDFSNA 300
            IA +NE+  EAEK K WSWR L+  IFGNENRD T +T+TGKSPDSYNL +K+PDFSNA
Sbjct: 247 KIANINEDGKEAEKNKPWSWRNLVSLIFGNENRDKTKRTRTGKSPDSYNLYDKTPDFSNA 306

Query: 301 YGKSVALDETEYSPLRDSRVGVYLVYLNAGSMMAPHVNPDADEFAIVTVGKGKMQITYPN 360
           YG SVALDE EYSPL  S +GVYLV L AGSMMAPH+NP A E+ IV  G G +QI YPN
Sbjct: 307 YGWSVALDEHEYSPLGHSGIGVYLVNLTAGSMMAPHINPTAAEYGIVLRGTGTIQIVYPN 366

Query: 361 GTSAFDAEVSEGNVIFFPKFFAFCQIASRTGPFEFVGFTTSSHRNHPQFLAGASSVYHTL 420
           GTSA D EV+EG+V + P++F FCQIASRTGPFEF GFTTSS RN PQFLAGA+S++HTL
Sbjct: 367 GTSAMDTEVTEGDVFWVPRYFPFCQIASRTGPFEFFGFTTSSRRNRPQFLAGANSIFHTL 426

Query: 421 RGPEMARAFDMAEDDLGRFLDAQSEAIILPSAEIAPPYKEEEKGRQEEEKRERRDREAIR 479
           R P +A AFD+ EDDL R L AQ E +ILPSAEIAPP+KEEEK R+ EE R  R+RE+ R
Sbjct: 427 RSPAVATAFDITEDDLDRLLSAQYEVVILPSAEIAPPHKEEEKRRRREEGRRERERESER 486

[Close]

BLAST of MS005051 vs. NCBI nr
Match: XP_022943526.1 (vicilin-like seed storage protein At2g28490 [Cucurbita moschata])

HSP 1 Score: 682.6 bits (1760), Expect = 2.6e-192
Identity = 349/483 (72.26%), Postives = 403/483 (83.44%), Query Frame = 0


Query: 1   MGKREALVVLLIIAVLGNAVGF----DREEEAEWERDRREEREFRREDRFLLEDSKHVIQ 60
           MGK+EALV+LLIIAVLGNA+G     + EEE EW R+R EEREFR ++RFLLEDSK VI+
Sbjct: 1   MGKKEALVMLLIIAVLGNAIGIKEEAEAEEEEEWWREREEEREFRSKERFLLEDSKRVIE 60

Query: 61  TEAGEMRVVKGPASRILDRQIHIGFITMEPKSLFIPQYMDSSLILFVQKGEVRLGLIYKD 120
           TEAGEMRV++ PASRILDR +HIGFITMEPKSLF+PQY+DSSLILFV++GEV++GLIYKD
Sbjct: 61  TEAGEMRVIRSPASRILDRPMHIGFITMEPKSLFVPQYLDSSLILFVRRGEVKVGLIYKD 120

Query: 121 ELAERRMKEGDVYRIPAGSVFYMVNIGEGQRLQIICSIDRAGSWSPGTVQSFFVAGGTDP 180
           ELAERRMK GDVYRIPAGSVFYMVN+GEGQRLQIICSID++ S S GT QSFFV GGT P
Sbjct: 121 ELAERRMKGGDVYRIPAGSVFYMVNVGEGQRLQIICSIDKSESLSYGTFQSFFVGGGTYP 180

Query: 181 ESILAGFDQDTLAAAFNVSRTELKRLMTRQRQGPIVYVSDTESPRVLSKFLQVKDEDRLN 240
            S+LAGFDQDTLA AFNVS TEL+R+++RQRQGPIVYVSDTESP V SKFLQVKD ++ N
Sbjct: 181 VSVLAGFDQDTLATAFNVSYTELRRILSRQRQGPIVYVSDTESPGVWSKFLQVKDGEKGN 240

Query: 241 TIAGVNEESGEAEKKKSWSWRKLLGSIFGNENRD-TPKTKTGKSPDSYNLREKSPDFSNA 300
            I+ +NE   EAEK K+WSWR L+ SIFGNENRD T +T+TGKSPDSYNL +K+PDFSNA
Sbjct: 241 QISNINENGEEAEKNKAWSWRNLMSSIFGNENRDKTKRTRTGKSPDSYNLYDKTPDFSNA 300

Query: 301 YGKSVALDETEYSPLRDSRVGVYLVYLNAGSMMAPHVNPDADEFAIVTVGKGKMQITYPN 360
           YG SVALDE EYSPL  S +GVYLV L AGSMMAPH+NP A E+ IV  G G +QI YPN
Sbjct: 301 YGWSVALDEHEYSPLGHSGIGVYLVNLTAGSMMAPHINPTAAEYGIVLRGTGTIQIVYPN 360

Query: 361 GTSAFDAEVSEGNVIFFPKFFAFCQIASRTGPFEFVGFTTSSHRNHPQFLAGASSVYHTL 420
           GTSA D EV+EG+V + P++F FCQIASRTGPFEF GFTTSS +N PQFLAGA+S++HTL
Sbjct: 361 GTSAMDTEVTEGDVFWVPRYFPFCQIASRTGPFEFFGFTTSSRKNRPQFLAGANSIFHTL 420

Query: 421 RGPEMARAFDMAEDDLGRFLDAQSEAIILPSAEIAPPYKEEEKGRQEEEKRERRDREAIR 479
           R   +A AFD+ EDDL R L  Q EA+ILPSAEIAPP+KEEEK R+ EE R  R+RE  R
Sbjct: 421 RSSAVATAFDITEDDLDRLLSLQYEAVILPSAEIAPPHKEEEKRRRREEGRREREREGER 480

[Close]

BLAST of MS005051 vs. ExPASy Swiss-Prot
Match: Q9SK09 (Vicilin-like seed storage protein At2g28490 OS=Arabidopsis thaliana OX=3702 GN=At2g28490 PE=2 SV=1)

HSP 1 Score: 409.8 bits (1052), Expect = 4.2e-113
Identity = 216/450 (48.00%), Postives = 302/450 (67.11%), Query Frame = 0


Query: 17  GNAVGFDREEEAEWERDRREEREFRREDRFLLEDSKHVIQTEAGEMRVVKGPASRILDRQ 76
           G A G + E   EW     E     R   F++ +S+ VI++E GEMRVV  P  RI+++ 
Sbjct: 58  GGAWGGEGEGGGEW-GGGGEGGGGGRRGWFMMRESRQVIKSEGGEMRVVLSPRGRIIEKP 117

Query: 77  IHIGFITMEPKSLFIPQYMDSSLILFVQKGEVRLGLIYKDELAERRMKEGDVYRIPAGSV 136
           +HIGF+TMEPK+LF+PQY+DSSL++F+++GE  LG+I KDE  ER++K GD+Y IPAGSV
Sbjct: 118 MHIGFLTMEPKTLFVPQYLDSSLLIFIRQGEATLGVICKDEFGERKLKAGDIYWIPAGSV 177

Query: 137 FYMVNIGEGQRLQIICSIDRAGSWSPGTVQSFFVAGGTDPESILAGFDQDTLAAAFNVSR 196
           FY+ N G GQRL +ICSID   S    T Q F++ GG  P S+LAGFD  TL +AFNVS 
Sbjct: 178 FYLHNTGLGQRLHVICSIDPTQSLGFETFQPFYIGGG--PSSVLAGFDPHTLTSAFNVSL 237

Query: 197 TELKRLMTRQRQGPIVYVSDTESPR----VLSKFLQVKDEDR---LNTIAGVNEESGEAE 256
            EL+++M  Q +GPIVYV++   P+    V ++FL ++ E++   L  +    + S + +
Sbjct: 238 PELQQMMMSQFRGPIVYVTEGPQPQPQSTVWTQFLGLRGEEKHKQLKKLLETKQGSPQDQ 297

Query: 257 KKKS-WSWRKLLGSIFGNENRDTPKTKTGKSPDSYNLREK--SPDFSNAYGKSVALDETE 316
           +  S WSWR ++ SI          + + +  DSYN+ +K   P F N YG S+ALD  +
Sbjct: 298 QYSSGWSWRNIVRSILDLTEEKNKGSGSSECEDSYNIYDKKDKPSFDNKYGWSIALDYDD 357

Query: 317 YSPLRDSRVGVYLVYLNAGSMMAPHVNPDADEFAIVTVGKGKMQITYPNGTSAFDAEVSE 376
           Y PL+ S +GVYLV L AG+MMAPH+NP A E+ IV  G G++Q+ +PNGTSA +  VS 
Sbjct: 358 YKPLKHSGIGVYLVNLTAGAMMAPHMNPTATEYGIVLAGSGEIQVVFPNGTSAMNTRVSV 417

Query: 377 GNVIFFPKFFAFCQIASRTGPFEFVGFTTSSHRNHPQFLAGASSVYHTLRGPEMARAFDM 436
           G+V + P++FAFCQIASRTGPFEFVGFTTS+H+N PQFL G++S+  TL    ++ AF +
Sbjct: 418 GDVFWIPRYFAFCQIASRTGPFEFVGFTTSAHKNRPQFLVGSNSLLRTLNLTSLSIAFGV 477

Query: 437 AEDDLGRFLDAQSEAIILPSAEIAPPYKEE 457
            E+ + RF++AQ EA+ILP+   APP+  E
Sbjct: 478 DEETMRRFIEAQREAVILPTPAAAPPHVGE 504

[Close]

BLAST of MS005051 vs. ExPASy Swiss-Prot
Match: F4IQK5 (Vicilin-like seed storage protein At2g18540 OS=Arabidopsis thaliana OX=3702 GN=At2g18540 PE=3 SV=1)

HSP 1 Score: 171.8 bits (434), Expect = 1.9e-41
Identity = 129/433 (29.79%), Postives = 213/433 (49.19%), Query Frame = 0


Query: 54  VIQTEAGEMRVVKGPASRILDRQIHIGFITMEPKSLFIPQYMDSSLILFVQKGEVRLGLI 113
           V+ TE G +  V+      +    HI FIT+EP +L +P  + S ++ FV  G   L  I
Sbjct: 53  VVATEFGNISAVQ------IGDGYHIQFITLEPNALLLPLLLHSDMVFFVHTGTGILNWI 112

Query: 114 YKDELAERRMKEGDVYRIPAGSVFYMVNIGEGQRLQIICSIDRAGSWSPGTVQSFFVAGG 173
            ++   +  ++ GDV+R+ +G+VFY V+  E  R+  I ++ +        +    +   
Sbjct: 113 DEESERKLELRRGDVFRLRSGTVFY-VHSNEKLRVYAIFNVGKC-------LNDPCLGAY 172

Query: 174 TDPESILAGFDQDTLAAAFNVSRTELKRLMTRQRQGPIVYVSDTESPRVLSKFLQVKDED 233
           +    +L GFD  TL +AF V    L++            + D   P ++   L      
Sbjct: 173 SSVRDLLLGFDDRTLRSAFAVPEDILRK------------IRDATKPPLIVNALP----- 232

Query: 234 RLNTIAGVNEESGEAEKKKSW-SWRKLLGSIFGNENRDTPKTKTGKSPDSYNLREKSPDF 293
             N   G+ E+  ++   + + S   +   +      DT K K+     ++N+ E+ PDF
Sbjct: 233 -RNRTQGLEEDKWQSRLVRLFVSVEDVTDHLAMKPIVDTNKKKS----RTFNVFEEDPDF 292

Query: 294 SNAYGKSVALDETEYSPLRDSRVGVYLVYLNAGSMMAPHVNPDADEFAIVTVGKGKMQIT 353
            N  G+S+ +DE +   L+ SR GV++V L  GSM+ PH NP A E +IV  G+G +++ 
Sbjct: 293 ENNNGRSIVVDEKDLDALKGSRFGVFMVNLTKGSMIGPHWNPSACEISIVLEGEGMVRVV 352

Query: 354 YPNGTSAFDAE-------VSEGNVIFFPKFFAFCQIASRTGPFEFVGFTTSSHRNHPQFL 413
                S+   +       V EG+V   PKF    Q++     F F+GF+TS+  NHPQFL
Sbjct: 353 NQQSLSSCKNDRKSESFMVEEGDVFVVPKFHPMAQMSFENSSFVFMGFSTSAKTNHPQFL 412

Query: 414 AGASSVYHTLRGPEMARAFDMAEDDLGRFLDAQSEAIILPSA-----EIAPPYKE-EEKG 473
            G SSV   L    +A +F+++ + +   L AQ E++I   A     E++   +E EE+ 
Sbjct: 413 VGQSSVLKVLDRDVVAVSFNLSNETIKGLLKAQKESVIFECASCAEGELSKLMREIEERK 449

[Close]

BLAST of MS005051 vs. ExPASy Swiss-Prot
Match: V9VGU0 (Vicilin Pin k 2.0101 OS=Pinus koraiensis OX=88728 PE=1 SV=1)

HSP 1 Score: 152.9 bits (385), Expect = 9.3e-36
Identity = 119/437 (27.23%), Postives = 196/437 (44.85%), Query Frame = 0


Query: 31  ERDRREEREFRREDRFLLEDSKHVIQTEAGEMRVVK--GPASRILD--RQIHIGFITMEP 90
           E +R E       DRF +  S     +EAGE+R +   G  S +L+   +  +  I M+P
Sbjct: 59  EEEREENPYVFHSDRFRIRAS-----SEAGEIRALPNFGEVSELLEGISRYRVTCIEMKP 118

Query: 91  KSLFIPQYMDSSLILFVQKGEVRLGLIYKDELAERRMKEGDVYRIPAGSVFYMVNIGEGQ 150
            ++ +P Y+D+  IL+V  G   +  + ++EL +R+++EGDV+ +P+G  FY+VN  +  
Sbjct: 119 NTVMLPHYIDAKWILYVTGGRGYIAYVQQNELVKRKLEEGDVFGVPSGHTFYLVNNDDHN 178

Query: 151 RLQIICSIDRAGSWSPGTVQSFFVAGGTDPESILAGFDQDTLAAAFNVSRTELKRLMTRQ 210
            L+ I S+ R  S   G  + F+VAGG +PE++ + F  D L AAFN    +L+ +    
Sbjct: 179 SLR-IASLLRTESTMRGEYEPFYVAGGRNPETVYSAFSDDVLEAAFNTDVQKLEHIFGAH 238

Query: 211 RQGPIVYVSDTESPRVLSKFLQVKDEDRLNTIAGVNEESGEAEKKKSWSWRKLLGSIFGN 270
           R+G I Y ++ +   ++ +              G + ES                     
Sbjct: 239 RRGVIFYANEEQIREMMRR-------------GGFSAES--------------------- 298

Query: 271 ENRDTPKTKTGKSPDSYNLREKSPDFSNAYGKSVALDETEYSPLRDSRVGVYLVYLNAGS 330
                  T   + P  +NLR + PDF N  G+       +   L    V V    LN G+
Sbjct: 299 -------TSASEQPKPFNLRNQKPDFENDNGRFTRAGPKDNPFLDSVDVTVGFGVLNPGT 358

Query: 331 MMAPHVNPDADEFAIVTVGKGKMQITYP-----NGTSA-------------FDAEVSEGN 390
           M AP  N  A   AIV  G+G++++  P     +G S+               A +  G 
Sbjct: 359 MTAPSHNTKATSIAIVMEGEGRIEMACPHLGQEHGWSSPRERGHQDINYERVRARLRTGT 418

Query: 391 VIFFPKFFAFCQIASRTGPFEFVGFTTSSHRNHPQFLAGASSVYHTLRGPEMARAFDMAE 445
           V   P      +IAS  G  E + F  ++  N  +FLAG ++V  TL       +F++  
Sbjct: 419 VYVVPAGHPITEIASTNGRLEILWFDINTSGNEREFLAGKNNVLQTLEKEVRHLSFNIPR 448

[Close]

BLAST of MS005051 vs. ExPASy Swiss-Prot
Match: F4JQG6 (Vicilin-like seed storage protein At4g36700 OS=Arabidopsis thaliana OX=3702 GN=At4g36700 PE=3 SV=1)

HSP 1 Score: 142.5 bits (358), Expect = 1.3e-32
Identity = 119/427 (27.87%), Postives = 196/427 (45.90%), Query Frame = 0


Query: 82  ITMEPKSLFIPQYMDSSLILFVQKGEVRLGLIYKDELAERRMKEGDVYRIPAGSVFYM-- 141
           IT+EP ++ +P  + S ++ FV  G   L  +  +E     ++ GDVYR+  GSVFY+  
Sbjct: 85  ITLEPNTILLPLLLHSDMVFFVDSGSGILNWV-DEEAKSTEIRLGDVYRLRPGSVFYLQS 144

Query: 142 --VNIGEGQRLQIICSIDRAGSWSPGTVQSFFVAGGTDPESILAGFDQDTLAAAFNVSRT 201
             V+I  G +L++        S +   +        +    ++ GFD+  L +AF V   
Sbjct: 145 KPVDIFLGTKLKLYAIF----SNNDECLHDPCFGAYSSITDLMFGFDETILQSAFGVPEG 204

Query: 202 ELKRLMTRQRQGPIVYVSDTESPRVLSKFLQVKDEDRLNTIAGVNEESGEAEKKKSWSWR 261
            ++  + R R  P + VS+T     ++   Q+  + RL  +   + +  + +KKK    +
Sbjct: 205 IIE--LMRNRTKPPLIVSETLCTPGVANTWQL--QPRLLKLFAGSADLVDNKKKKEKKEK 264

Query: 262 KLLGSIFGNENRDTPKTKTGKSPDSYNLREKSPDFSNAYGKSVALDETEYSPLRDSRVGV 321
           K              K K  K   ++N+ E  PDF + YG+++ ++  +   L+ S VGV
Sbjct: 265 K-------------EKVKKAK---TFNVFESEPDFESPYGRTITINRKDLKVLKGSMVGV 324

Query: 322 YLVYLNAGSMMAPHVNPDADEFAIVTVGKGKMQITYPNGTSAFDAE-------VSEGNVI 381
            +V L  GSMM PH NP A E +IV  G G +++   + +S   +E       V EG++ 
Sbjct: 325 SMVNLTQGSMMGPHWNPWACEISIVLKGAGMVRVLRSSISSNTSSECKNVRFKVEEGDIF 384

Query: 382 FFPKFFAFCQIASRTGPFEFVGFTTSSHRNHPQFLAGASSVYHTLRGPEMARAFDMAEDD 441
             P+     Q++       FVGFTTS+  N PQFLAG  S    L    +A + +++   
Sbjct: 385 AVPRLHPMAQMSFNNDSLVFVGFTTSAKNNEPQFLAGEDSALRMLDRQVLAASLNVSSVT 444

Query: 442 LGRFLDAQSEAIILPSAEIA-------------------------PPYKEEEKGRQEEEK 473
           +   L AQ EA+IL     A                            KEEE+ ++EEE+
Sbjct: 445 IDGLLGAQKEAVILECHSCAEGEIEKLKVEIERKKIDDERKRRHDERKKEEEEAKREEEE 486

[Close]

BLAST of MS005051 vs. ExPASy Swiss-Prot
Match: B4X640 (Vicilin Pis v 3.0101 OS=Pistacia vera OX=55513 PE=1 SV=2)

HSP 1 Score: 138.3 bits (347), Expect = 2.4e-31
Identity = 115/462 (24.89%), Postives = 198/462 (42.86%), Query Frame = 0


Query: 22  FDREEEAEWERDRREEREFRREDRFLLEDSKHVIQTEAGEMRVVKGP----ASRILD--R 81
           + R+EE E E D  EE+E   E+ ++ ED     + +  + +VV  P     S++L    
Sbjct: 133 YSRDEEEEEEGD--EEQEEEDENPYVFEDEHFTTRVKTEQGKVVVLPKFTKRSKLLRGLE 192

Query: 82  QIHIGFITMEPKSLFIPQYMDSSLILFVQKGEVRLGLIYKDELAERRMKEGDVYRIPAGS 141
           +  + F+   P++  +P +MD+  I FV  G   +  I +++     +K+GD+ RI AG+
Sbjct: 193 KYRLAFLVANPQAFVVPNHMDADSIFFVSWGRGTITKIRENKRESMNVKQGDIIRIRAGT 252

Query: 142 VFYMVNIGEGQRLQIICSIDRAGSWSPGTVQSFFVAGGTDPESILAGFDQDTLAAAFNVS 201
            FY+VN  E ++L I+  +       PG  + F   GG +PES    F ++ L AA    
Sbjct: 253 PFYIVNTDENEKLYIVKLLQPVN--LPGHYEVFHGPGGENPESFYRAFSREVLEAALKTP 312

Query: 202 RTELKRLMTRQRQGPIVYVSDTESPRVLSKFLQVKDEDRLNTIAGVNEESGEAEKKKSWS 261
           R +L++L  +Q +G IV  S  E  R +S+                       E    W 
Sbjct: 313 RDKLEKLFEKQDEGAIVKAS-KEQIRAMSR---------------------RGEGPSIWP 372

Query: 262 WRKLLGSIFGNENRDTPKTKTGKSPDSYNLREKSPDFSNAYGKSVALDETEYSPLRDSRV 321
           +                   TGKS  ++NL +K P  SN YG+    +  +Y PL++  +
Sbjct: 373 F-------------------TGKSTGTFNLFKKDPSQSNNYGQLFESEFKDYPPLQELDI 432

Query: 322 GVYLVYLNAGSMMAPHVNPDADEFAIVTVGKGKMQITYPNGTSA--------------FD 381
            V  V +  G M  P  N  A + AIV  G+G+++I  P+ +S+                
Sbjct: 433 MVSYVNITKGGMSGPFYNSRATKIAIVVSGEGRLEIACPHLSSSKNSGQEKSGPSYKKLS 492

Query: 382 AEVSEGNVIFFPKFFAFCQIASRTGPFEFVGFTTSSHRNHPQFLAGASSVYHTLRGPEMA 441
           + +   +V   P    F  +AS     E + F  ++  N    LAG  ++   +      
Sbjct: 493 SSIRTDSVFVVPAGHPFVTVASGNQNLEILCFEVNAEGNIRYTLAGKKNIIEVMEKEAKE 545

Query: 442 RAFDMAEDDLGRFLDAQSEAIILPSAEIAPPYKEEEKGRQEE 464
            AF    +++ +    Q E          P +++ ++GR +E
Sbjct: 553 LAFKTKGEEVDKVFGKQDEEFFFQ----GPKWRQHQQGRADE 545

[Close]

BLAST of MS005051 vs. ExPASy TrEMBL
Match: A0A6J1CI43 (vicilin-like seed storage protein At2g28490 OS=Momordica charantia OX=3673 GN=LOC111011142 PE=4 SV=1)

HSP 1 Score: 957.2 bits (2473), Expect = 2.6e-275
Identity = 486/487 (99.79%), Postives = 487/487 (100.00%), Query Frame = 0


Query: 1   MGKREALVVLLIIAVLGNAVGFDREEEAEWERDRREEREFRREDRFLLEDSKHVIQTEAG 60
           MGKREALVVLLIIAVLGNA+GFDREEEAEWERDRREEREFRREDRFLLEDSKHVIQTEAG
Sbjct: 31  MGKREALVVLLIIAVLGNAIGFDREEEAEWERDRREEREFRREDRFLLEDSKHVIQTEAG 90

Query: 61  EMRVVKGPASRILDRQIHIGFITMEPKSLFIPQYMDSSLILFVQKGEVRLGLIYKDELAE 120
           EMRVVKGPASRILDRQIHIGFITMEPKSLFIPQYMDSSLILFVQKGEVRLGLIYKDELAE
Sbjct: 91  EMRVVKGPASRILDRQIHIGFITMEPKSLFIPQYMDSSLILFVQKGEVRLGLIYKDELAE 150

Query: 121 RRMKEGDVYRIPAGSVFYMVNIGEGQRLQIICSIDRAGSWSPGTVQSFFVAGGTDPESIL 180
           RRMKEGDVYRIPAGSVFYMVNIGEGQRLQIICSIDRAGSWSPGTVQSFFVAGGTDPESIL
Sbjct: 151 RRMKEGDVYRIPAGSVFYMVNIGEGQRLQIICSIDRAGSWSPGTVQSFFVAGGTDPESIL 210

Query: 181 AGFDQDTLAAAFNVSRTELKRLMTRQRQGPIVYVSDTESPRVLSKFLQVKDEDRLNTIAG 240
           AGFDQDTLAAAFNVSRTELKRLMTRQRQGPIVYVSDTESPRVLSKFLQVKDEDRLNTIAG
Sbjct: 211 AGFDQDTLAAAFNVSRTELKRLMTRQRQGPIVYVSDTESPRVLSKFLQVKDEDRLNTIAG 270

Query: 241 VNEESGEAEKKKSWSWRKLLGSIFGNENRDTPKTKTGKSPDSYNLREKSPDFSNAYGKSV 300
           VNEESGEAEKKKSWSWRKLLGSIFGNENRDTPKTKTGKSPDSYNLREKSPDFSNAYGKSV
Sbjct: 271 VNEESGEAEKKKSWSWRKLLGSIFGNENRDTPKTKTGKSPDSYNLREKSPDFSNAYGKSV 330

Query: 301 ALDETEYSPLRDSRVGVYLVYLNAGSMMAPHVNPDADEFAIVTVGKGKMQITYPNGTSAF 360
           ALDETEYSPLRDSRVGVYLVYLNAGSMMAPHVNPDADEFAIVTVGKGKMQITYPNGTSAF
Sbjct: 331 ALDETEYSPLRDSRVGVYLVYLNAGSMMAPHVNPDADEFAIVTVGKGKMQITYPNGTSAF 390

Query: 361 DAEVSEGNVIFFPKFFAFCQIASRTGPFEFVGFTTSSHRNHPQFLAGASSVYHTLRGPEM 420
           DAEVSEGNVIFFPKFFAFCQIASRTGPFEFVGFTTSSHRNHPQFLAGASSVYHTLRGPEM
Sbjct: 391 DAEVSEGNVIFFPKFFAFCQIASRTGPFEFVGFTTSSHRNHPQFLAGASSVYHTLRGPEM 450

Query: 421 ARAFDMAEDDLGRFLDAQSEAIILPSAEIAPPYKEEEKGRQEEEKRERRDREAIRSLEGN 480
           ARAFDMAEDDLGRFLDAQSEAIILPSAEIAPPYKEEEKGRQEEEKRERRDREAIRSLEGN
Sbjct: 451 ARAFDMAEDDLGRFLDAQSEAIILPSAEIAPPYKEEEKGRQEEEKRERRDREAIRSLEGN 510

Query: 481 IMGMGFD 488
           IMGMGFD
Sbjct: 511 IMGMGFD 517

[Close]

BLAST of MS005051 vs. ExPASy TrEMBL
Match: A0A6J1J8R6 (vicilin-like seed storage protein At2g28490 OS=Cucurbita maxima OX=3661 GN=LOC111483630 PE=4 SV=1)

HSP 1 Score: 683.3 bits (1762), Expect = 7.3e-193
Identity = 350/483 (72.46%), Postives = 402/483 (83.23%), Query Frame = 0


Query: 1   MGKREALVVLLIIAVLGNAVGFDRE----EEAEWERDRREEREFRREDRFLLEDSKHVIQ 60
           M K+EALV+LLIIAVLGNA+G   E    EE EW R+R EEREFR ++RFLLEDSK VI+
Sbjct: 7   MWKKEALVMLLIIAVLGNAIGIKEEAEAAEEEEWWREREEEREFRSKERFLLEDSKRVIE 66

Query: 61  TEAGEMRVVKGPASRILDRQIHIGFITMEPKSLFIPQYMDSSLILFVQKGEVRLGLIYKD 120
           TEAGEMRV++ PASRILDR +HIGFITMEPKSLF+PQY+DSSLILFV++GEV++GLIYKD
Sbjct: 67  TEAGEMRVIRSPASRILDRPMHIGFITMEPKSLFVPQYLDSSLILFVRRGEVKVGLIYKD 126

Query: 121 ELAERRMKEGDVYRIPAGSVFYMVNIGEGQRLQIICSIDRAGSWSPGTVQSFFVAGGTDP 180
           ELAERRMK GDVYRIPAGSVFYMVN+GEGQRLQIICSID++ S S GT QSFF+ GGT P
Sbjct: 127 ELAERRMKGGDVYRIPAGSVFYMVNVGEGQRLQIICSIDKSESLSYGTFQSFFIGGGTYP 186

Query: 181 ESILAGFDQDTLAAAFNVSRTELKRLMTRQRQGPIVYVSDTESPRVLSKFLQVKDEDRLN 240
            S+LAGFDQDTLA AFNVS TEL+R+++RQRQGPIVYVSDTESP V SKFLQVKD D+ N
Sbjct: 187 VSVLAGFDQDTLATAFNVSYTELRRILSRQRQGPIVYVSDTESPGVWSKFLQVKDGDKGN 246

Query: 241 TIAGVNEESGEAEKKKSWSWRKLLGSIFGNENRD-TPKTKTGKSPDSYNLREKSPDFSNA 300
            IA +NE+  EAEK K WSWR L+  IFGNENRD T +T+TGKSPDSYNL +K+PDFSNA
Sbjct: 247 KIANINEDGKEAEKNKPWSWRNLVSLIFGNENRDKTKRTRTGKSPDSYNLYDKTPDFSNA 306

Query: 301 YGKSVALDETEYSPLRDSRVGVYLVYLNAGSMMAPHVNPDADEFAIVTVGKGKMQITYPN 360
           YG SVALDE EYSPL  S +GVYLV L AGSMMAPH+NP A E+ IV  G G +QI YPN
Sbjct: 307 YGWSVALDEHEYSPLGHSGIGVYLVNLTAGSMMAPHINPTAAEYGIVLRGTGTIQIVYPN 366

Query: 361 GTSAFDAEVSEGNVIFFPKFFAFCQIASRTGPFEFVGFTTSSHRNHPQFLAGASSVYHTL 420
           GTSA D EV+EG+V + P++F FCQIASRTGPFEF GFTTSS RN PQFLAGA+S++HTL
Sbjct: 367 GTSAMDTEVTEGDVFWVPRYFPFCQIASRTGPFEFFGFTTSSRRNRPQFLAGANSIFHTL 426

Query: 421 RGPEMARAFDMAEDDLGRFLDAQSEAIILPSAEIAPPYKEEEKGRQEEEKRERRDREAIR 479
           R P +A AFD+ EDDL R L AQ E +ILPSAEIAPP+KEEEK R+ EE R  R+RE+ R
Sbjct: 427 RSPAVATAFDITEDDLDRLLSAQYEVVILPSAEIAPPHKEEEKRRRREEGRRERERESER 486

[Close]

BLAST of MS005051 vs. ExPASy TrEMBL
Match: A0A6J1FRY9 (vicilin-like seed storage protein At2g28490 OS=Cucurbita moschata OX=3662 GN=LOC111448275 PE=4 SV=1)

HSP 1 Score: 682.6 bits (1760), Expect = 1.2e-192
Identity = 349/483 (72.26%), Postives = 403/483 (83.44%), Query Frame = 0


Query: 1   MGKREALVVLLIIAVLGNAVGF----DREEEAEWERDRREEREFRREDRFLLEDSKHVIQ 60
           MGK+EALV+LLIIAVLGNA+G     + EEE EW R+R EEREFR ++RFLLEDSK VI+
Sbjct: 1   MGKKEALVMLLIIAVLGNAIGIKEEAEAEEEEEWWREREEEREFRSKERFLLEDSKRVIE 60

Query: 61  TEAGEMRVVKGPASRILDRQIHIGFITMEPKSLFIPQYMDSSLILFVQKGEVRLGLIYKD 120
           TEAGEMRV++ PASRILDR +HIGFITMEPKSLF+PQY+DSSLILFV++GEV++GLIYKD
Sbjct: 61  TEAGEMRVIRSPASRILDRPMHIGFITMEPKSLFVPQYLDSSLILFVRRGEVKVGLIYKD 120

Query: 121 ELAERRMKEGDVYRIPAGSVFYMVNIGEGQRLQIICSIDRAGSWSPGTVQSFFVAGGTDP 180
           ELAERRMK GDVYRIPAGSVFYMVN+GEGQRLQIICSID++ S S GT QSFFV GGT P
Sbjct: 121 ELAERRMKGGDVYRIPAGSVFYMVNVGEGQRLQIICSIDKSESLSYGTFQSFFVGGGTYP 180

Query: 181 ESILAGFDQDTLAAAFNVSRTELKRLMTRQRQGPIVYVSDTESPRVLSKFLQVKDEDRLN 240
            S+LAGFDQDTLA AFNVS TEL+R+++RQRQGPIVYVSDTESP V SKFLQVKD ++ N
Sbjct: 181 VSVLAGFDQDTLATAFNVSYTELRRILSRQRQGPIVYVSDTESPGVWSKFLQVKDGEKGN 240

Query: 241 TIAGVNEESGEAEKKKSWSWRKLLGSIFGNENRD-TPKTKTGKSPDSYNLREKSPDFSNA 300
            I+ +NE   EAEK K+WSWR L+ SIFGNENRD T +T+TGKSPDSYNL +K+PDFSNA
Sbjct: 241 QISNINENGEEAEKNKAWSWRNLMSSIFGNENRDKTKRTRTGKSPDSYNLYDKTPDFSNA 300

Query: 301 YGKSVALDETEYSPLRDSRVGVYLVYLNAGSMMAPHVNPDADEFAIVTVGKGKMQITYPN 360
           YG SVALDE EYSPL  S +GVYLV L AGSMMAPH+NP A E+ IV  G G +QI YPN
Sbjct: 301 YGWSVALDEHEYSPLGHSGIGVYLVNLTAGSMMAPHINPTAAEYGIVLRGTGTIQIVYPN 360

Query: 361 GTSAFDAEVSEGNVIFFPKFFAFCQIASRTGPFEFVGFTTSSHRNHPQFLAGASSVYHTL 420
           GTSA D EV+EG+V + P++F FCQIASRTGPFEF GFTTSS +N PQFLAGA+S++HTL
Sbjct: 361 GTSAMDTEVTEGDVFWVPRYFPFCQIASRTGPFEFFGFTTSSRKNRPQFLAGANSIFHTL 420

Query: 421 RGPEMARAFDMAEDDLGRFLDAQSEAIILPSAEIAPPYKEEEKGRQEEEKRERRDREAIR 479
           R   +A AFD+ EDDL R L  Q EA+ILPSAEIAPP+KEEEK R+ EE R  R+RE  R
Sbjct: 421 RSSAVATAFDITEDDLDRLLSLQYEAVILPSAEIAPPHKEEEKRRRREEGRREREREGER 480

[Close]

BLAST of MS005051 vs. ExPASy TrEMBL
Match: A0A1S3CKT5 (LOW QUALITY PROTEIN: vicilin-like seed storage protein At2g28490 OS=Cucumis melo OX=3656 GN=LOC103501928 PE=4 SV=1)

HSP 1 Score: 679.1 bits (1751), Expect = 1.4e-191
Identity = 350/502 (69.72%), Postives = 414/502 (82.47%), Query Frame = 0


Query: 1   MGKREALVVLLIIAVLGNAVGFDREEEAEWERDRREEREFRREDRFLLEDSKHVIQTEAG 60
           MGK+EAL++LLI+AVLGNA+G   EEE EW R+R EEREF R++RFL+ DSK VI+TEAG
Sbjct: 43  MGKKEALLILLIVAVLGNAIGIKEEEEEEWWREREEEREFGRKERFLMVDSKKVIETEAG 102

Query: 61  EMRVVKGPASRILDRQIHIGFITMEPKSLFIPQYMDSSLILFVQKGEVRLGLIYKDELAE 120
           EMRV++GPASRILDR +HIGFITMEPKSLF+PQY+DS+LILFV++G+V++GLIYKDELAE
Sbjct: 103 EMRVMRGPASRILDRPMHIGFITMEPKSLFVPQYLDSTLILFVRRGDVKVGLIYKDELAE 162

Query: 121 RRMKEGDVYRIPAGSVFYMVNIGEGQRLQIICSIDRAGSWSPGTVQSFFVAGGTDPESIL 180
           RRMK GDV+RIPAGSVFYMVN+GEGQRL+IICSID++ S S GT QSFFVAGG  P S+L
Sbjct: 163 RRMKGGDVFRIPAGSVFYMVNVGEGQRLEIICSIDKSESLSYGTFQSFFVAGGKYPGSVL 222

Query: 181 AGFDQDTLAAAFNVSRTELKRLMTRQRQGPIVYVSDTESPRVLSKFLQVKDEDRLNTIAG 240
           AGFDQDTLA AFNVS TEL+R+++RQRQGPIVYVSDTESPRV SKFLQV DE RL+ +A 
Sbjct: 223 AGFDQDTLATAFNVSYTELRRILSRQRQGPIVYVSDTESPRVWSKFLQVNDEARLSKVAD 282

Query: 241 VNEESGEAEKKKSWSWRKLLGSIFGNENRD-TPKTKTGKSPDSYNLREKSPDFSNAYGKS 300
           ++E+  ++EK K WSWRKL+ SIF N NRD + KT+TGKSPDSYNL +K PDFSNAYG S
Sbjct: 283 IDEDGEKSEKNKPWSWRKLVESIFRNGNRDKSKKTRTGKSPDSYNLYDKDPDFSNAYGWS 342

Query: 301 VALDETEYSPLRDSRVGVYLVYLNAGSMMAPHVNPDADEFAIVTVGKGKMQITYPNGTSA 360
           VALDETEY PLR S +GVYLV L AGSMMAPHVNP A E+ IV  G G +QI YPNGTSA
Sbjct: 343 VALDETEYHPLRHSGIGVYLVNLTAGSMMAPHVNPTASEYGIVLRGTGTIQIVYPNGTSA 402

Query: 361 FDAEVSEGNVIFFPKFFAFCQIASRTGPFEFVGFTTSSHRNHPQFLAGASSVYHTLRGPE 420
            +AEV+EG+V + P++F FCQIASRTGPFEF GFTTS+ +N PQFLAGASS++HTLR  E
Sbjct: 403 MNAEVTEGDVFWIPRYFPFCQIASRTGPFEFFGFTTSARKNRPQFLAGASSIFHTLRSME 462

Query: 421 MARAFDMAEDDLGRFLDAQSEAIILPSAEIAPPYKEEEKGRQEEE--------------- 480
           MA AFD+ EDD+ R L AQ EAIILPSAEIAPP+KEEEK R++EE               
Sbjct: 463 MATAFDITEDDMERLLGAQYEAIILPSAEIAPPHKEEEKRRRKEEERREAEWERERERER 522

Query: 481 KRERRDREAIRSLEGNIMGMGF 487
           +RERR  E +RS   N++ MGF
Sbjct: 523 ERERRVDEMVRSFGRNLV-MGF 543

[Close]

BLAST of MS005051 vs. ExPASy TrEMBL
Match: Q39651 (PreproMP27-MP32 OS=Cucurbita hybrid cultivar OX=170850 PE=2 SV=1)

HSP 1 Score: 678.3 bits (1749), Expect = 2.3e-191
Identity = 348/483 (72.05%), Postives = 401/483 (83.02%), Query Frame = 0


Query: 1   MGKREALVVLLIIAVLGNAVGFDRE----EEAEWERDRREEREFRREDRFLLEDSKHVIQ 60
           M K+EALV+LLIIAVLGNA+G   E    EE EW R+R EEREFR +++FLLEDSK VI+
Sbjct: 7   MWKKEALVMLLIIAVLGNAIGIKEEAEAAEEEEWWREREEEREFRSKEQFLLEDSKRVIE 66

Query: 61  TEAGEMRVVKGPASRILDRQIHIGFITMEPKSLFIPQYMDSSLILFVQKGEVRLGLIYKD 120
           TEAGEMRV++ PASRILDR +HIGFITMEPKSLF+PQY+DSSLILFV++GEV++GLIYKD
Sbjct: 67  TEAGEMRVIRSPASRILDRPMHIGFITMEPKSLFVPQYLDSSLILFVRRGEVKVGLIYKD 126

Query: 121 ELAERRMKEGDVYRIPAGSVFYMVNIGEGQRLQIICSIDRAGSWSPGTVQSFFVAGGTDP 180
           ELAERRMK GDVYRIPAGSVFYMVN+GEGQRLQIICSID++ S S GT QSFF+ GGT P
Sbjct: 127 ELAERRMKGGDVYRIPAGSVFYMVNVGEGQRLQIICSIDKSESLSYGTFQSFFIGGGTYP 186

Query: 181 ESILAGFDQDTLAAAFNVSRTELKRLMTRQRQGPIVYVSDTESPRVLSKFLQVKDEDRLN 240
            S+LAGFDQDTLA AFNVS TEL+R+++RQRQGPIVYVSDTESP V SKFLQVKD D+ N
Sbjct: 187 VSVLAGFDQDTLATAFNVSYTELRRILSRQRQGPIVYVSDTESPGVWSKFLQVKDGDKGN 246

Query: 241 TIAGVNEESGEAEKKKSWSWRKLLGSIFGNENRD-TPKTKTGKSPDSYNLREKSPDFSNA 300
            IA +NE+  EAEK K WSWR L+  IFGNENRD T +T+TGKSPDSYNL +K+PDFSNA
Sbjct: 247 KIANINEDGEEAEKNKPWSWRNLVSLIFGNENRDKTKRTRTGKSPDSYNLYDKTPDFSNA 306

Query: 301 YGKSVALDETEYSPLRDSRVGVYLVYLNAGSMMAPHVNPDADEFAIVTVGKGKMQITYPN 360
           YG SVALDE EYSPL  S +GVYLV L AGSMMAPH+NP A E+ IV  G G +QI YPN
Sbjct: 307 YGWSVALDEHEYSPLGHSGIGVYLVNLTAGSMMAPHINPTAAEYGIVLRGTGTIQIVYPN 366

Query: 361 GTSAFDAEVSEGNVIFFPKFFAFCQIASRTGPFEFVGFTTSSHRNHPQFLAGASSVYHTL 420
           GTSA D EV+EG+V + P++F FCQIASRTGPFEF GFTTSS RN PQFLA A+S++HTL
Sbjct: 367 GTSAMDTEVTEGDVFWVPRYFPFCQIASRTGPFEFFGFTTSSRRNRPQFLACANSIFHTL 426

Query: 421 RGPEMARAFDMAEDDLGRFLDAQSEAIILPSAEIAPPYKEEEKGRQEEEKRERRDREAIR 479
           R P +A AFD+ EDDL R L AQ E +ILPSAEIAPP+KEEEK R+ EE R  R+RE+ R
Sbjct: 427 RSPAVATAFDITEDDLDRLLSAQYEVVILPSAEIAPPHKEEEKRRRREEGRRERERESER 486

[Close]

BLAST of MS005051 vs. TAIR 10
Match: AT2G28490.1 (RmlC-like cupins superfamily protein )

HSP 1 Score: 409.8 bits (1052), Expect = 3.0e-114
Identity = 216/450 (48.00%), Postives = 302/450 (67.11%), Query Frame = 0


Query: 17  GNAVGFDREEEAEWERDRREEREFRREDRFLLEDSKHVIQTEAGEMRVVKGPASRILDRQ 76
           G A G + E   EW     E     R   F++ +S+ VI++E GEMRVV  P  RI+++ 
Sbjct: 58  GGAWGGEGEGGGEW-GGGGEGGGGGRRGWFMMRESRQVIKSEGGEMRVVLSPRGRIIEKP 117

Query: 77  IHIGFITMEPKSLFIPQYMDSSLILFVQKGEVRLGLIYKDELAERRMKEGDVYRIPAGSV 136
           +HIGF+TMEPK+LF+PQY+DSSL++F+++GE  LG+I KDE  ER++K GD+Y IPAGSV
Sbjct: 118 MHIGFLTMEPKTLFVPQYLDSSLLIFIRQGEATLGVICKDEFGERKLKAGDIYWIPAGSV 177

Query: 137 FYMVNIGEGQRLQIICSIDRAGSWSPGTVQSFFVAGGTDPESILAGFDQDTLAAAFNVSR 196
           FY+ N G GQRL +ICSID   S    T Q F++ GG  P S+LAGFD  TL +AFNVS 
Sbjct: 178 FYLHNTGLGQRLHVICSIDPTQSLGFETFQPFYIGGG--PSSVLAGFDPHTLTSAFNVSL 237

Query: 197 TELKRLMTRQRQGPIVYVSDTESPR----VLSKFLQVKDEDR---LNTIAGVNEESGEAE 256
            EL+++M  Q +GPIVYV++   P+    V ++FL ++ E++   L  +    + S + +
Sbjct: 238 PELQQMMMSQFRGPIVYVTEGPQPQPQSTVWTQFLGLRGEEKHKQLKKLLETKQGSPQDQ 297

Query: 257 KKKS-WSWRKLLGSIFGNENRDTPKTKTGKSPDSYNLREK--SPDFSNAYGKSVALDETE 316
           +  S WSWR ++ SI          + + +  DSYN+ +K   P F N YG S+ALD  +
Sbjct: 298 QYSSGWSWRNIVRSILDLTEEKNKGSGSSECEDSYNIYDKKDKPSFDNKYGWSIALDYDD 357

Query: 317 YSPLRDSRVGVYLVYLNAGSMMAPHVNPDADEFAIVTVGKGKMQITYPNGTSAFDAEVSE 376
           Y PL+ S +GVYLV L AG+MMAPH+NP A E+ IV  G G++Q+ +PNGTSA +  VS 
Sbjct: 358 YKPLKHSGIGVYLVNLTAGAMMAPHMNPTATEYGIVLAGSGEIQVVFPNGTSAMNTRVSV 417

Query: 377 GNVIFFPKFFAFCQIASRTGPFEFVGFTTSSHRNHPQFLAGASSVYHTLRGPEMARAFDM 436
           G+V + P++FAFCQIASRTGPFEFVGFTTS+H+N PQFL G++S+  TL    ++ AF +
Sbjct: 418 GDVFWIPRYFAFCQIASRTGPFEFVGFTTSAHKNRPQFLVGSNSLLRTLNLTSLSIAFGV 477

Query: 437 AEDDLGRFLDAQSEAIILPSAEIAPPYKEE 457
            E+ + RF++AQ EA+ILP+   APP+  E
Sbjct: 478 DEETMRRFIEAQREAVILPTPAAAPPHVGE 504

[Close]

BLAST of MS005051 vs. TAIR 10
Match: AT2G18540.1 (RmlC-like cupins superfamily protein )

HSP 1 Score: 171.8 bits (434), Expect = 1.4e-42
Identity = 129/433 (29.79%), Postives = 213/433 (49.19%), Query Frame = 0


Query: 54  VIQTEAGEMRVVKGPASRILDRQIHIGFITMEPKSLFIPQYMDSSLILFVQKGEVRLGLI 113
           V+ TE G +  V+      +    HI FIT+EP +L +P  + S ++ FV  G   L  I
Sbjct: 53  VVATEFGNISAVQ------IGDGYHIQFITLEPNALLLPLLLHSDMVFFVHTGTGILNWI 112

Query: 114 YKDELAERRMKEGDVYRIPAGSVFYMVNIGEGQRLQIICSIDRAGSWSPGTVQSFFVAGG 173
            ++   +  ++ GDV+R+ +G+VFY V+  E  R+  I ++ +        +    +   
Sbjct: 113 DEESERKLELRRGDVFRLRSGTVFY-VHSNEKLRVYAIFNVGKC-------LNDPCLGAY 172

Query: 174 TDPESILAGFDQDTLAAAFNVSRTELKRLMTRQRQGPIVYVSDTESPRVLSKFLQVKDED 233
           +    +L GFD  TL +AF V    L++            + D   P ++   L      
Sbjct: 173 SSVRDLLLGFDDRTLRSAFAVPEDILRK------------IRDATKPPLIVNALP----- 232

Query: 234 RLNTIAGVNEESGEAEKKKSW-SWRKLLGSIFGNENRDTPKTKTGKSPDSYNLREKSPDF 293
             N   G+ E+  ++   + + S   +   +      DT K K+     ++N+ E+ PDF
Sbjct: 233 -RNRTQGLEEDKWQSRLVRLFVSVEDVTDHLAMKPIVDTNKKKS----RTFNVFEEDPDF 292

Query: 294 SNAYGKSVALDETEYSPLRDSRVGVYLVYLNAGSMMAPHVNPDADEFAIVTVGKGKMQIT 353
            N  G+S+ +DE +   L+ SR GV++V L  GSM+ PH NP A E +IV  G+G +++ 
Sbjct: 293 ENNNGRSIVVDEKDLDALKGSRFGVFMVNLTKGSMIGPHWNPSACEISIVLEGEGMVRVV 352

Query: 354 YPNGTSAFDAE-------VSEGNVIFFPKFFAFCQIASRTGPFEFVGFTTSSHRNHPQFL 413
                S+   +       V EG+V   PKF    Q++     F F+GF+TS+  NHPQFL
Sbjct: 353 NQQSLSSCKNDRKSESFMVEEGDVFVVPKFHPMAQMSFENSSFVFMGFSTSAKTNHPQFL 412

Query: 414 AGASSVYHTLRGPEMARAFDMAEDDLGRFLDAQSEAIILPSA-----EIAPPYKE-EEKG 473
            G SSV   L    +A +F+++ + +   L AQ E++I   A     E++   +E EE+ 
Sbjct: 413 VGQSSVLKVLDRDVVAVSFNLSNETIKGLLKAQKESVIFECASCAEGELSKLMREIEERK 449

[Close]

BLAST of MS005051 vs. TAIR 10
Match: AT4G36700.1 (RmlC-like cupins superfamily protein )

HSP 1 Score: 142.5 bits (358), Expect = 8.9e-34
Identity = 119/427 (27.87%), Postives = 196/427 (45.90%), Query Frame = 0


Query: 82  ITMEPKSLFIPQYMDSSLILFVQKGEVRLGLIYKDELAERRMKEGDVYRIPAGSVFYM-- 141
           IT+EP ++ +P  + S ++ FV  G   L  +  +E     ++ GDVYR+  GSVFY+  
Sbjct: 85  ITLEPNTILLPLLLHSDMVFFVDSGSGILNWV-DEEAKSTEIRLGDVYRLRPGSVFYLQS 144

Query: 142 --VNIGEGQRLQIICSIDRAGSWSPGTVQSFFVAGGTDPESILAGFDQDTLAAAFNVSRT 201
             V+I  G +L++        S +   +        +    ++ GFD+  L +AF V   
Sbjct: 145 KPVDIFLGTKLKLYAIF----SNNDECLHDPCFGAYSSITDLMFGFDETILQSAFGVPEG 204

Query: 202 ELKRLMTRQRQGPIVYVSDTESPRVLSKFLQVKDEDRLNTIAGVNEESGEAEKKKSWSWR 261
            ++  + R R  P + VS+T     ++   Q+  + RL  +   + +  + +KKK    +
Sbjct: 205 IIE--LMRNRTKPPLIVSETLCTPGVANTWQL--QPRLLKLFAGSADLVDNKKKKEKKEK 264

Query: 262 KLLGSIFGNENRDTPKTKTGKSPDSYNLREKSPDFSNAYGKSVALDETEYSPLRDSRVGV 321
           K              K K  K   ++N+ E  PDF + YG+++ ++  +   L+ S VGV
Sbjct: 265 K-------------EKVKKAK---TFNVFESEPDFESPYGRTITINRKDLKVLKGSMVGV 324

Query: 322 YLVYLNAGSMMAPHVNPDADEFAIVTVGKGKMQITYPNGTSAFDAE-------VSEGNVI 381
            +V L  GSMM PH NP A E +IV  G G +++   + +S   +E       V EG++ 
Sbjct: 325 SMVNLTQGSMMGPHWNPWACEISIVLKGAGMVRVLRSSISSNTSSECKNVRFKVEEGDIF 384

Query: 382 FFPKFFAFCQIASRTGPFEFVGFTTSSHRNHPQFLAGASSVYHTLRGPEMARAFDMAEDD 441
             P+     Q++       FVGFTTS+  N PQFLAG  S    L    +A + +++   
Sbjct: 385 AVPRLHPMAQMSFNNDSLVFVGFTTSAKNNEPQFLAGEDSALRMLDRQVLAASLNVSSVT 444

Query: 442 LGRFLDAQSEAIILPSAEIA-------------------------PPYKEEEKGRQEEEK 473
           +   L AQ EA+IL     A                            KEEE+ ++EEE+
Sbjct: 445 IDGLLGAQKEAVILECHSCAEGEIEKLKVEIERKKIDDERKRRHDERKKEEEEAKREEEE 486

[Close]

BLAST of MS005051 vs. TAIR 10
Match: AT1G03890.1 (RmlC-like cupins superfamily protein )

HSP 1 Score: 84.7 bits (208), Expect = 2.2e-16
Identity = 95/426 (22.30%), Postives = 171/426 (40.14%), Query Frame = 0


Query: 58  EAGEMRVVKGPASRILDRQIHIGFITMEPKSLFIPQYMDSSLILFVQKGEVRLGLIYK-- 117
           EAG+M V    +  +    + +  IT++P S+F+P +     + +V +GE  +G I    
Sbjct: 53  EAGQMEVWDHMSPELRCAGVTVARITLQPNSIFLPAFFSPPALAYVVQGEGVMGTIASGC 112

Query: 118 -DELAE------------------------RRMKEGDVYRIPAGSVFYMVNIGEGQR-LQ 177
            +  AE                           + GDV+   AG   +  N G+    + 
Sbjct: 113 PETFAEVEGSSGRGGGGDPGRRFEDMHQKLENFRRGDVFASLAGVSQWWYNRGDSDAVIV 172

Query: 178 IICSIDRAGSWSPGTVQSFFVAGGTDPE-----------SILAGFDQDTLAAAFNVSRTE 237
           I+  +    +      + F +AG    E           +  +GFD + +A AF ++   
Sbjct: 173 IVLDVTNRENQLDQVPRMFQLAGSRTQEEEQPLTWPSGNNAFSGFDPNIIAEAFKINIET 232

Query: 238 LKRLMT-RQRQGPIVYVSDTESPRVLSKFLQVKDEDRLNTIAGVNEESGEAEKKKSWSWR 297
            K+L   +  +G I+  +       L   +    E + + IA   EE        ++   
Sbjct: 233 AKQLQNQKDNRGNIIRANGP-----LHFVIPPPREWQQDGIANGIEE--------TYCTA 292

Query: 298 KLLGSIFGNENRDTPKTKTGKSPDSYNLREKSPDFSNAYGKSVALDETEYSPLRDSRVGV 357
           K+      +EN D P              E+S  FS   G+   L+      LR  R+  
Sbjct: 293 KI------HENIDDP--------------ERSDHFSTRAGRISTLNSLNLPVLRLVRLNA 352

Query: 358 YLVYLNAGSMMAPHVNPDADEFAIVTVGKGKMQITYPNGTSAFDAEVSEGNVIFFPKFFA 417
              YL +G M+ P    +A     VT G+ K+Q+   NG S F+ +V +G +I  P+ FA
Sbjct: 353 LRGYLYSGGMVLPQWTANAHTVLYVTGGQAKIQVVDDNGQSVFNEQVGQGQIIVIPQGFA 412

Query: 418 FCQIASRTGPFEFVGFTTSSHRNHPQFLAGASSVYHTLRGPEMARAFDMAEDDLGRFLDA 444
             + A  TG FE++ F T+ +  +   L+G +S    +    +  ++ + E++  R   +
Sbjct: 413 VSKTAGETG-FEWISFKTNDNA-YINTLSGQTSYLRAVPVDVIKASYGVNEEEAKRIKFS 443

[Close]

BLAST of MS005051 vs. TAIR 10
Match: AT5G44120.3 (RmlC-like cupins superfamily protein )

HSP 1 Score: 63.9 bits (154), Expect = 4.0e-10
Identity = 86/419 (20.53%), Postives = 165/419 (39.38%), Query Frame = 0


Query: 50  DSKHVIQTEAGEMRVVKGPASRILDRQIHIGFITMEPKSLFIPQYMDSSLILFVQKGEVR 109
           +  HV+++EAG + V    A ++    +      +E K L++P + +++ + FV KG   
Sbjct: 45  EPSHVLKSEAGRIEVWDHHAPQLRCSGVSFARYIIESKGLYLPSFFNTAKLSFVAKGRGL 104

Query: 110 LGLIY---------KDELAER------------------RMKEGDVYRIPAGSVFYMVNI 169
           +G +            E   R                   ++ GD      G   +  N 
Sbjct: 105 MGKVIPGCAETFQDSSEFQPRFEGQGQSQRFRDMHQKVEHIRSGDTIATTPGVAQWFYND 164

Query: 170 GEGQRLQIICSIDRAGSWS--PGTVQSFFVAGGTDPESI-LAGFDQDTLAAAFNVSRTEL 229
           G+ + L I+   D A   +      + F++AG      + L G +Q      FN      
Sbjct: 165 GQ-EPLVIVSVFDLASHQNQLDRNPRPFYLAGNNPQGQVWLQGREQQPQKNIFN------ 224

Query: 230 KRLMTRQRQGPIVYVSDTESPRVLSKFLQVKDEDRLNTIA-----GVNEESGEAEKKKSW 289
                    GP V     +     ++ LQ +D++R N +      GV       ++ +  
Sbjct: 225 -------GFGPEVIAQALKIDLQTAQQLQNQDDNRGNIVRVQGPFGVIRPPLRGQRPQEE 284

Query: 290 SWRKLLGSIFGNENRDTPKTKTGKSPDSYNLREKSPDFSNAYGKSVALDETEYSPLRDSR 349
              +      GN   +T    + +  D+ +   ++  +    G    L+  +   LR  R
Sbjct: 285 EEEEGRHGRHGNGLEET--ICSARCTDNLDDPSRADVYKPQLGYISTLNSYDLPILRFIR 344

Query: 350 VGVYLVYLNAGSMMAPHVNPDADEFAIVTVGKGKMQITYPNGTSAFDAEVSEGNVIFFPK 409
           +      +   +M+ P  N +A+    VT G+ ++QI   NG   FD +VS+G +I  P+
Sbjct: 345 LSALRGSIRQNAMVLPQWNANANAILYVTDGEAQIQIVNDNGNRVFDGQVSQGQLIAVPQ 404

Query: 410 FFAFCQIASRTGPFEFVGFTTSSHRNHPQFLAGASSVYHTLRGPEMARAFDMAEDDLGR 434
            F+  + A+ +  F++V F T+++      LAG +SV   L    +   F ++ ++  R
Sbjct: 405 GFSVVKRAT-SNRFQWVEFKTNANA-QINTLAGRTSVLRGLPLEVITNGFQISPEEARR 445

The following BLAST results are available for this feature:

BLAST of MS005051 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022140488.1	5.4e-275	99.79	vicilin-like seed storage protein At2g28490, partial [Momordica charantia]	[more]
XP_023511963.1	4.7e-194	72.67	vicilin-like seed storage protein At2g28490 [Cucurbita pepo subsp. pepo]	[more]
KAG6570767.1	3.0e-193	72.26	Vicilin-like seed storage protein, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_022985621.1	1.5e-192	72.46	vicilin-like seed storage protein At2g28490 [Cucurbita maxima]	[more]
XP_022943526.1	2.6e-192	72.26	vicilin-like seed storage protein At2g28490 [Cucurbita moschata]	[more]

BLAST of MS005051 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SK09	4.2e-113	48.00	Vicilin-like seed storage protein At2g28490 OS=Arabidopsis thaliana OX=3702 GN=A...	[more]
F4IQK5	1.9e-41	29.79	Vicilin-like seed storage protein At2g18540 OS=Arabidopsis thaliana OX=3702 GN=A...	[more]
V9VGU0	9.3e-36	27.23	Vicilin Pin k 2.0101 OS=Pinus koraiensis OX=88728 PE=1 SV=1	[more]
F4JQG6	1.3e-32	27.87	Vicilin-like seed storage protein At4g36700 OS=Arabidopsis thaliana OX=3702 GN=A...	[more]
B4X640	2.4e-31	24.89	Vicilin Pis v 3.0101 OS=Pistacia vera OX=55513 PE=1 SV=2	[more]

BLAST of MS005051 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CI43	2.6e-275	99.79	vicilin-like seed storage protein At2g28490 OS=Momordica charantia OX=3673 GN=LO...	[more]
A0A6J1J8R6	7.3e-193	72.46	vicilin-like seed storage protein At2g28490 OS=Cucurbita maxima OX=3661 GN=LOC11...	[more]
A0A6J1FRY9	1.2e-192	72.26	vicilin-like seed storage protein At2g28490 OS=Cucurbita moschata OX=3662 GN=LOC...	[more]
A0A1S3CKT5	1.4e-191	69.72	LOW QUALITY PROTEIN: vicilin-like seed storage protein At2g28490 OS=Cucumis melo...	[more]
Q39651	2.3e-191	72.05	PreproMP27-MP32 OS=Cucurbita hybrid cultivar OX=170850 PE=2 SV=1	[more]

BLAST of MS005051 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G28490.1	3.0e-114	48.00	RmlC-like cupins superfamily protein	[more]
AT2G18540.1	1.4e-42	29.79	RmlC-like cupins superfamily protein	[more]
AT4G36700.1	8.9e-34	27.87	RmlC-like cupins superfamily protein	[more]
AT1G03890.1	2.2e-16	22.30	RmlC-like cupins superfamily protein	[more]
AT5G44120.3	4.0e-10	20.53	RmlC-like cupins superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006045	Cupin 1	SMART	SM00835	Cupin_1_3	coord: 283..432 e-value: 6.2E-41 score: 152.0 coord: 46..200 e-value: 1.1E-23 score: 94.7
IPR006045	Cupin 1	PFAM	PF00190	Cupin_1	coord: 283..428 e-value: 1.0E-29 score: 103.1 coord: 49..198 e-value: 3.4E-12 score: 46.2
IPR014710	RmlC-like jelly roll fold	GENE3D	2.60.120.10	Jelly Rolls	coord: 271..447 e-value: 2.2E-43 score: 149.8
IPR014710	RmlC-like jelly roll fold	GENE3D	2.60.120.10	Jelly Rolls	coord: 35..231 e-value: 9.3E-55 score: 187.1
None	No IPR available	PIRSR	PIRSR617774-2	PIRSR617774-2	coord: 224..376 e-value: 1.3E-5 score: 22.1
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 451..476
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 446..487
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 265..289
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 271..285
None	No IPR available	PANTHER	PTHR31189:SF2	OS03G0336100 PROTEIN	coord: 4..454
None	No IPR available	PANTHER	PTHR31189	OS03G0336100 PROTEIN-RELATED	coord: 4..454
None	No IPR available	CDD	cd02245	cupin_7S_vicilin-like_C	coord: 290..441 e-value: 2.26131E-49 score: 164.997
None	No IPR available	CDD	cd02244	cupin_7S_vicilin-like_N	coord: 54..225 e-value: 4.05981E-76 score: 234.71
IPR011051	RmlC-like cupin domain superfamily	SUPERFAMILY	51182	RmlC-like cupins	coord: 46..440

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS005051.1	MS005051.1	mRNA