MS005049 (gene) Bitter gourd (TR) v1

Overview


Name	MS005049
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	(R)-specific enoyl-CoA hydratase
Location	scaffold176: 2336725 .. 2337679 (-)
RNA-Seq Expression	MS005049
Synteny	MS005049

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCTAAGAAGAGGTGTATTCTCAGCTTTTATTGTTTCTTCTTCTTTGAAATTCTTCTCTTCTTCAACTTCTAATGTGCTCAAAGTCGGGGATATCTTAAGCCATACAAGAATATTTACTAGTGAAGATGTGTTGGAATACTCCAAGGTGAGTCATGACTCGAATCCGCTGCATTTTGATTCTGCATTAGCCCGGCGTGCTGGATATGAAGATCGTCTTGTTCATGGGATGCTTGTTGCTTCAATGTTCCCTCATATCATTTCCTCTCATTTTGTAAGTCTAGCCACTCTGCATTTCTTACTTTGAAAGTATATTTTGATTTGTCTAGTTCACATAAAAGGGAATTATTGTTGGTACCGTTCTAAACCAAGAGCCAAGAGGAATCCTTGCTCGGCCTGTATCACTAAGAGTAGGAGAAGAGTTTCAAAGTTTCAACGACAACAAAGGAATTTGATAGAAATCGAAAGTCTCTCGACATAAAAGCTCATCATCGAGCATCTGAAATCTCTGGGAACTTTAAGGAAACTGAAATGGCTTCTGAAACTGTCTTCTTACAACTGATTTACGTGTGTTTAAGCCATTTTAGTGTATGGTCTATAATCTGAAGATGATTCTGACTCATGGAATATGTTGAGGATGATTGTAGCCAGGGGCCATCTACGTATCGCAAAGCTTGAATTTTAAGTTACCAGTATACGTTGGAGAGCAGATCATTGGCCAGGTAGAAGCAGTCAACTTGAAAGAAAATAAGAAGAGATACCTGTAAGCTCTTGTTGTTGGCAAATCTCTGACTTCTATAATTCAAATACCAAGTTATCAGTTCATTGAGTTGTGTAATTTGCGAATGTGTTTCAGGGCAAAATTCAGAACATTGTGCTTGAGGAACGGCGGGGACCTTGTTCTTGAGGGTGAGGCAATGGCCATTTTACCTTCTGATTTTCATCCCACAGACAGA

mRNA sequence

ATGCTAAGAAGAGGTGTATTCTCAGCTTTTATTGTTTCTTCTTCTTTGAAATTCTTCTCTTCTTCAACTTCTAATGTGCTCAAAGTCGGGGATATCTTAAGCCATACAAGAATATTTACTAGTGAAGATGTGTTGGAATACTCCAAGGTGAGTCATGACTCGAATCCGCTGCATTTTGATTCTGCATTAGCCCGGCGTGCTGGATATGAAGATCGTCTTGTTCATGGGATGCTTGTTGCTTCAATGTTCCCTCATATCATTTCCTCTCATTTTCCAGGGGCCATCTACGTATCGCAAAGCTTGAATTTTAAGTTACCAGTATACGTTGGAGAGCAGATCATTGGCCAGGTAGAAGCAGTCAACTTGAAAGAAAATAAGAAGAGATACCTGGCAAAATTCAGAACATTGTGCTTGAGGAACGGCGGGGACCTTGTTCTTGAGGGTGAGGCAATGGCCATTTTACCTTCTGATTTTCATCCCACAGACAGA

Coding sequence (CDS)

ATGCTAAGAAGAGGTGTATTCTCAGCTTTTATTGTTTCTTCTTCTTTGAAATTCTTCTCTTCTTCAACTTCTAATGTGCTCAAAGTCGGGGATATCTTAAGCCATACAAGAATATTTACTAGTGAAGATGTGTTGGAATACTCCAAGGTGAGTCATGACTCGAATCCGCTGCATTTTGATTCTGCATTAGCCCGGCGTGCTGGATATGAAGATCGTCTTGTTCATGGGATGCTTGTTGCTTCAATGTTCCCTCATATCATTTCCTCTCATTTTCCAGGGGCCATCTACGTATCGCAAAGCTTGAATTTTAAGTTACCAGTATACGTTGGAGAGCAGATCATTGGCCAGGTAGAAGCAGTCAACTTGAAAGAAAATAAGAAGAGATACCTGGCAAAATTCAGAACATTGTGCTTGAGGAACGGCGGGGACCTTGTTCTTGAGGGTGAGGCAATGGCCATTTTACCTTCTGATTTTCATCCCACAGACAGA

Protein sequence

MLRRGVFSAFIVSSSLKFFSSSTSNVLKVGDILSHTRIFTSEDVLEYSKVSHDSNPLHFDSALARRAGYEDRLVHGMLVASMFPHIISSHFPGAIYVSQSLNFKLPVYVGEQIIGQVEAVNLKENKKRYLAKFRTLCLRNGGDLVLEGEAMAILPSDFHPTDR

Homology

[Close]

BLAST of MS005049 vs. NCBI nr
Match: XP_022140510.1 (uncharacterized protein LOC111011158 [Momordica charantia])

HSP 1 Score: 318.5 bits (815), Expect = 3.3e-83
Identity = 162/163 (99.39%), Postives = 162/163 (99.39%), Query Frame = 0


Query: 1   MLRRGVFSAFIVSSSLKFFSSSTSNVLKVGDILSHTRIFTSEDVLEYSKVSHDSNPLHFD 60
           MLRRGVFSAFIVSSSLKFFSSSTSNVLKVGDILSHTRIFTSEDVLEYSKVSHDSNPLHFD
Sbjct: 1   MLRRGVFSAFIVSSSLKFFSSSTSNVLKVGDILSHTRIFTSEDVLEYSKVSHDSNPLHFD 60

Query: 61  SALARRAGYEDRLVHGMLVASMFPHIISSHFPGAIYVSQSLNFKLPVYVGEQIIGQVEAV 120
           SALARRAGYEDRLVHGMLVASMFP IISSHFPGAIYVSQSLNFKLPVYVGEQIIGQVEAV
Sbjct: 61  SALARRAGYEDRLVHGMLVASMFPLIISSHFPGAIYVSQSLNFKLPVYVGEQIIGQVEAV 120

Query: 121 NLKENKKRYLAKFRTLCLRNGGDLVLEGEAMAILPSDFHPTDR 164
           NLKENKKRYLAKFRTLCLRNGGDLVLEGEAMAILPSDFHPTDR
Sbjct: 121 NLKENKKRYLAKFRTLCLRNGGDLVLEGEAMAILPSDFHPTDR 163

[Close]

BLAST of MS005049 vs. NCBI nr
Match: XP_038901209.1 ((R)-specific enoyl-CoA hydratase [Benincasa hispida])

HSP 1 Score: 264.6 bits (675), Expect = 5.6e-67
Identity = 134/162 (82.72%), Postives = 148/162 (91.36%), Query Frame = 0


Query: 1   MLRRGVFSAFIVSSSLKFFSSSTSNVLKVGDILSHTRIFTSEDVLEYSKVSHDSNPLHFD 60
           ML RG+FS  I S+SLK FSSSTSNVLKVGDILS+TRIFTS+DVLEYSKVSHDSNPLHFD
Sbjct: 1   MLSRGLFSMHI-STSLKCFSSSTSNVLKVGDILSYTRIFTSKDVLEYSKVSHDSNPLHFD 60

Query: 61  SALARRAGYEDRLVHGMLVASMFPHIISSHFPGAIYVSQSLNFKLPVYVGEQIIGQVEAV 120
           S LARRAG+  RLVHGMLVASMFPHIISSHFPGAIYVSQSLNFKLPVYVGE I+GQVEA+
Sbjct: 61  SELARRAGFNGRLVHGMLVASMFPHIISSHFPGAIYVSQSLNFKLPVYVGENIVGQVEAI 120

Query: 121 NLKENKKRYLAKFRTLCLRNGGDLVLEGEAMAILPSDFHPTD 163
           +L+ENKKRYLAKF T C+RNG ++VLEGEA AI+PSDFH T+
Sbjct: 121 DLRENKKRYLAKFITKCMRNGHEVVLEGEARAIIPSDFHSTN 161

[Close]

BLAST of MS005049 vs. NCBI nr
Match: XP_022985629.1 (uncharacterized protein LOC111483634 [Cucurbita maxima])

HSP 1 Score: 262.3 bits (669), Expect = 2.8e-66
Identity = 134/162 (82.72%), Postives = 146/162 (90.12%), Query Frame = 0


Query: 1   MLRRGVFSAFIVSSSLKFFSSSTSNVLKVGDILSHTRIFTSEDVLEYSKVSHDSNPLHFD 60
           ML RG   +  +SSSLK FSSSTSNVLKVGDILSHTRIFTSEDVLEYSKVSHDSNPLHFD
Sbjct: 1   MLSRGGLISSYISSSLKCFSSSTSNVLKVGDILSHTRIFTSEDVLEYSKVSHDSNPLHFD 60

Query: 61  SALARRAGYEDRLVHGMLVASMFPHIISSHFPGAIYVSQSLNFKLPVYVGEQIIGQVEAV 120
           SALARRAG++DRLVHGMLVASMFP +ISSHFPGAIYVSQSLNFKLPVYVGE II +VEA+
Sbjct: 61  SALARRAGFDDRLVHGMLVASMFPRVISSHFPGAIYVSQSLNFKLPVYVGETIIVRVEAI 120

Query: 121 NLKENKKRYLAKFRTLCLRNGGDLVLEGEAMAILPSDFHPTD 163
           +L+E KKRYLAKF+T CLRNG +LVL+GEA AILPSDF  TD
Sbjct: 121 DLREIKKRYLAKFQTKCLRNGDELVLDGEARAILPSDFCSTD 162

[Close]

BLAST of MS005049 vs. NCBI nr
Match: XP_023512018.1 (uncharacterized protein LOC111776855 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 261.5 bits (667), Expect = 4.7e-66
Identity = 133/162 (82.10%), Postives = 146/162 (90.12%), Query Frame = 0


Query: 1   MLRRGVFSAFIVSSSLKFFSSSTSNVLKVGDILSHTRIFTSEDVLEYSKVSHDSNPLHFD 60
           ML RG   +  +SSSLK FSSSTSNVLKVGDILSHTRIFTSEDVLEYSKVSHDSNPLHFD
Sbjct: 1   MLSRGGLVSSYISSSLKCFSSSTSNVLKVGDILSHTRIFTSEDVLEYSKVSHDSNPLHFD 60

Query: 61  SALARRAGYEDRLVHGMLVASMFPHIISSHFPGAIYVSQSLNFKLPVYVGEQIIGQVEAV 120
           SALARRAG++DRLVHGMLVASMFPH+ISSHFPGAIYVSQSLNFKLPVYVGE I+ +VEA+
Sbjct: 61  SALARRAGFDDRLVHGMLVASMFPHVISSHFPGAIYVSQSLNFKLPVYVGETIMVRVEAI 120

Query: 121 NLKENKKRYLAKFRTLCLRNGGDLVLEGEAMAILPSDFHPTD 163
           +L+E KKRYLAKF+T CLRNG +LVL+GEA AILPS F  TD
Sbjct: 121 DLREIKKRYLAKFQTKCLRNGDELVLDGEARAILPSGFCSTD 162

[Close]

BLAST of MS005049 vs. NCBI nr
Match: KAG7010611.1 ((R)-specific enoyl-CoA hydratase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 260.8 bits (665), Expect = 8.0e-66
Identity = 133/162 (82.10%), Postives = 146/162 (90.12%), Query Frame = 0


Query: 1   MLRRGVFSAFIVSSSLKFFSSSTSNVLKVGDILSHTRIFTSEDVLEYSKVSHDSNPLHFD 60
           ML RG   +  +SSSLK FSSSTSNVLKVGDILSHTRIFTSEDVLEYSKVSHDSNPLHFD
Sbjct: 17  MLSRGGLISSYISSSLKCFSSSTSNVLKVGDILSHTRIFTSEDVLEYSKVSHDSNPLHFD 76

Query: 61  SALARRAGYEDRLVHGMLVASMFPHIISSHFPGAIYVSQSLNFKLPVYVGEQIIGQVEAV 120
           SALARRAG++DRLVHGMLVASMFPH+ISSHFPGAIYVSQSLNFKLPVYVGE II +VEA+
Sbjct: 77  SALARRAGFDDRLVHGMLVASMFPHVISSHFPGAIYVSQSLNFKLPVYVGETIIVRVEAI 136

Query: 121 NLKENKKRYLAKFRTLCLRNGGDLVLEGEAMAILPSDFHPTD 163
           +L+E KKR+LAKF+T CLRNG +LVL+GEA AILPS F  TD
Sbjct: 137 DLREIKKRHLAKFQTKCLRNGDELVLDGEARAILPSGFCSTD 178

[Close]

BLAST of MS005049 vs. ExPASy Swiss-Prot
Match: O32472 ((R)-specific enoyl-CoA hydratase OS=Aeromonas caviae OX=648 GN=phaJ PE=1 SV=1)

HSP 1 Score: 99.0 bits (245), Expect = 5.3e-20
Identity = 52/131 (39.69%), Postives = 79/131 (60.31%), Query Frame = 0


Query: 27  LKVGDILSHTRIFTSEDVLEYSKVSHDSNPLHFDSALARRAGYEDRLVHGMLVASMFPHI 86
           L+VG     ++ F + +V  ++ +S D NPLH D A A    +E  +VHGML+AS+F  +
Sbjct: 6   LEVGQKARLSKRFGAAEVAAFAALSEDFNPLHLDPAFAATTAFERPIVHGMLLASLFSGL 65

Query: 87  ISSHFP--GAIYVSQSLNFKLPVYVGEQIIGQVEAVNLKENKKRYLAKFRTLCLRNGGDL 146
           +    P  G+IY+ QSL+FKLPV+VG+++  +VE   L+E+K   +A   T     GG L
Sbjct: 66  LGQQLPGKGSIYLGQSLSFKLPVFVGDEVTAEVEVTALREDKP--IATLTTRIFTQGGAL 125

Query: 147 VLEGEAMAILP 156
            + GEA+  LP
Sbjct: 126 AVTGEAVVKLP 134

[Close]

BLAST of MS005049 vs. ExPASy Swiss-Prot
Match: Q2RQ36 ((R)-specific enoyl-CoA hydratase OS=Rhodospirillum rubrum (strain ATCC 11170 / ATH 1.1.1 / DSM 467 / LMG 4362 / NCIMB 8255 / S1) OX=269796 GN=phaJ PE=1 SV=1)

HSP 1 Score: 82.0 bits (201), Expect = 6.7e-15
Identity = 45/132 (34.09%), Postives = 70/132 (53.03%), Query Frame = 0


Query: 27  LKVGDILSHTRIFTSEDVLEYSKVSHDSNPLHFDSALARRAGYEDRLVHGMLVASMFPHI 86
           +K G   S  +  +  D+  ++ +S D+NP H +   A+   ++ R+ HGML A     +
Sbjct: 14  IKEGQSASLAKTISESDIYLFAGLSMDTNPAHVNEDYAQTTVFKTRIAHGMLSAGFISAV 73

Query: 87  ISSHF--PGAIYVSQSLNFKLPVYVGEQIIGQVEAVNLKENKKRYLAKFRTLCLRNGGDL 146
           + +    PGAIYV+QSL FK PV +G+ +   V    L   KK     FRT C    G +
Sbjct: 74  LGTRLPGPGAIYVNQSLKFKAPVRIGDTVTATVTVTGLVPEKK--FVTFRTTC-TVAGKV 133

Query: 147 VLEGEAMAILPS 157
           V+EGEA  ++P+
Sbjct: 134 VIEGEATVMVPA 142

[Close]

BLAST of MS005049 vs. ExPASy Swiss-Prot
Match: C5AZ74 (3-hydroxybutyryl-CoA dehydratase OS=Methylorubrum extorquens (strain ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1) OX=272630 GN=croR PE=4 SV=1)

HSP 1 Score: 77.4 bits (189), Expect = 1.7e-13
Identity = 38/118 (32.20%), Postives = 69/118 (58.47%), Query Frame = 0


Query: 40  TSEDVLEYSKVSHDSNPLHFDSALARRAGYEDRLVHGMLVASMFPHIISSHF--PGAIYV 99
           +S DV+ +++++ D NP+H     A R  +  R+ HG+  A +   ++ +    PGA+Y+
Sbjct: 26  SSSDVVGFAEITGDRNPIHLSEHFAARTPFGTRIAHGLYTAGLISAVLGTRLPGPGAVYI 85

Query: 100 SQSLNFKLPVYVGEQIIGQVEAVNLKENKKRYLAKFRTLCLRNGGDLVLEGEAMAILP 156
           SQ+LNF+ PV +G+ +  +VE   L   ++R  A+    C   G ++VL+GEA+  +P
Sbjct: 86  SQTLNFRAPVRIGDIVEVKVEVAELIPERRR--ARLACTC-SVGDEVVLDGEALVKVP 140

[Close]

BLAST of MS005049 vs. ExPASy Swiss-Prot
Match: P86397 (Hydroxyacyl-thioester dehydratase type 2, mitochondrial OS=Homo sapiens OX=9606 GN=HTD2 PE=1 SV=1)

HSP 1 Score: 75.5 bits (184), Expect = 6.3e-13
Identity = 39/132 (29.55%), Postives = 70/132 (53.03%), Query Frame = 0


Query: 27  LKVGDILSHTRIFTSEDVLEYSKVSHDSNPLHFDSALARRAGYEDRLVHGMLVASMFPHI 86
           +KVGD     R FT  DV  +S+++ D NPLH +   A+   + + +VHG+L+  +   +
Sbjct: 36  IKVGDRAELRRAFTQTDVATFSELTGDVNPLHLNEDFAKHTKFGNTIVHGVLINGLISAL 95

Query: 87  ISSHF--PGAIYVSQSLNFKLPVYVGEQIIGQVEAVNLKENKKRYLAKFRTLC-LRNGGD 146
           + +    PG +++SQ ++F  P+Y+GE ++   E   L    KR++A     C +     
Sbjct: 96  LGTKMPGPGCVFLSQEISFPAPLYIGEVVLASAEVKKL----KRFIAIIAVSCSVIESKK 155

Query: 147 LVLEGEAMAILP 156
            V+EG    ++P
Sbjct: 156 TVMEGWVKVMVP 163

[Close]

BLAST of MS005049 vs. ExPASy Swiss-Prot
Match: Q5AV07 (Fatty acid synthase beta subunit aflB OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) OX=227321 GN=atnM PE=2 SV=1)

HSP 1 Score: 45.4 bits (106), Expect = 7.0e-04
Identity = 30/100 (30.00%), Postives = 49/100 (49.00%), Query Frame = 0


Query: 40   TSEDVLEYSKVSHDSNPLHFDSALARRAGY-EDRLVHGMLVASMFPHIISSHFPG---AI 99
            TS D  +Y+  S DSNP+H   A AR AG+ E R++HGM  + +   ++  H  G     
Sbjct: 1609 TSSD--QYAAASGDSNPIHLSRAFARYAGHNEGRVIHGMQTSGLVRGVVELHAAGNDPRR 1668

Query: 100  YVSQSLNFKLPVYVGEQIIGQVEAVNLKENKKRYLAKFRT 136
              + S +FK  V  GE ++  +    +   +   +A  R+
Sbjct: 1669 MKAWSASFKGKVSPGETLLVDISHTGMNNGRLIVVATARS 1706

[Close]

BLAST of MS005049 vs. ExPASy TrEMBL
Match: A0A6J1CFA0 (uncharacterized protein LOC111011158 OS=Momordica charantia OX=3673 GN=LOC111011158 PE=4 SV=1)

HSP 1 Score: 318.5 bits (815), Expect = 1.6e-83
Identity = 162/163 (99.39%), Postives = 162/163 (99.39%), Query Frame = 0


Query: 1   MLRRGVFSAFIVSSSLKFFSSSTSNVLKVGDILSHTRIFTSEDVLEYSKVSHDSNPLHFD 60
           MLRRGVFSAFIVSSSLKFFSSSTSNVLKVGDILSHTRIFTSEDVLEYSKVSHDSNPLHFD
Sbjct: 1   MLRRGVFSAFIVSSSLKFFSSSTSNVLKVGDILSHTRIFTSEDVLEYSKVSHDSNPLHFD 60

Query: 61  SALARRAGYEDRLVHGMLVASMFPHIISSHFPGAIYVSQSLNFKLPVYVGEQIIGQVEAV 120
           SALARRAGYEDRLVHGMLVASMFP IISSHFPGAIYVSQSLNFKLPVYVGEQIIGQVEAV
Sbjct: 61  SALARRAGYEDRLVHGMLVASMFPLIISSHFPGAIYVSQSLNFKLPVYVGEQIIGQVEAV 120

Query: 121 NLKENKKRYLAKFRTLCLRNGGDLVLEGEAMAILPSDFHPTDR 164
           NLKENKKRYLAKFRTLCLRNGGDLVLEGEAMAILPSDFHPTDR
Sbjct: 121 NLKENKKRYLAKFRTLCLRNGGDLVLEGEAMAILPSDFHPTDR 163

[Close]

BLAST of MS005049 vs. ExPASy TrEMBL
Match: A0A6J1JDU3 (uncharacterized protein LOC111483634 OS=Cucurbita maxima OX=3661 GN=LOC111483634 PE=4 SV=1)

HSP 1 Score: 262.3 bits (669), Expect = 1.3e-66
Identity = 134/162 (82.72%), Postives = 146/162 (90.12%), Query Frame = 0


Query: 1   MLRRGVFSAFIVSSSLKFFSSSTSNVLKVGDILSHTRIFTSEDVLEYSKVSHDSNPLHFD 60
           ML RG   +  +SSSLK FSSSTSNVLKVGDILSHTRIFTSEDVLEYSKVSHDSNPLHFD
Sbjct: 1   MLSRGGLISSYISSSLKCFSSSTSNVLKVGDILSHTRIFTSEDVLEYSKVSHDSNPLHFD 60

Query: 61  SALARRAGYEDRLVHGMLVASMFPHIISSHFPGAIYVSQSLNFKLPVYVGEQIIGQVEAV 120
           SALARRAG++DRLVHGMLVASMFP +ISSHFPGAIYVSQSLNFKLPVYVGE II +VEA+
Sbjct: 61  SALARRAGFDDRLVHGMLVASMFPRVISSHFPGAIYVSQSLNFKLPVYVGETIIVRVEAI 120

Query: 121 NLKENKKRYLAKFRTLCLRNGGDLVLEGEAMAILPSDFHPTD 163
           +L+E KKRYLAKF+T CLRNG +LVL+GEA AILPSDF  TD
Sbjct: 121 DLREIKKRYLAKFQTKCLRNGDELVLDGEARAILPSDFCSTD 162

[Close]

BLAST of MS005049 vs. ExPASy TrEMBL
Match: A0A0A0KCN3 (MaoC-like domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G290840 PE=4 SV=1)

HSP 1 Score: 260.0 bits (663), Expect = 6.6e-66
Identity = 131/162 (80.86%), Postives = 147/162 (90.74%), Query Frame = 0


Query: 1   MLRRGVFSAFIVSSSLKFFSSSTSNVLKVGDILSHTRIFTSEDVLEYSKVSHDSNPLHFD 60
           ML +G+FS +I SSSLK FSSSTS+VLKVGDILS+ RIFTSEDVLEYSKVSHDSNPLHFD
Sbjct: 1   MLSKGLFSTYI-SSSLKCFSSSTSSVLKVGDILSYNRIFTSEDVLEYSKVSHDSNPLHFD 60

Query: 61  SALARRAGYEDRLVHGMLVASMFPHIISSHFPGAIYVSQSLNFKLPVYVGEQIIGQVEAV 120
           S LA+RAG+   LVHG+LVASMFPHIISSH+PGAIYVSQSLNFKLPVYVGE+I+GQVEA+
Sbjct: 61  SKLAQRAGFNGCLVHGLLVASMFPHIISSHYPGAIYVSQSLNFKLPVYVGEKIVGQVEAI 120

Query: 121 NLKENKKRYLAKFRTLCLRNGGDLVLEGEAMAILPSDFHPTD 163
            L+ENKKRYLAKF+T CLRNG +LVLEGEA AILP DFH T+
Sbjct: 121 ELRENKKRYLAKFKTKCLRNGDELVLEGEARAILPVDFHSTN 161

[Close]

BLAST of MS005049 vs. ExPASy TrEMBL
Match: A0A5A7T147 ((R)-specific enoyl-CoA hydratase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold84G00540 PE=4 SV=1)

HSP 1 Score: 258.8 bits (660), Expect = 1.5e-65
Identity = 132/162 (81.48%), Postives = 145/162 (89.51%), Query Frame = 0


Query: 1   MLRRGVFSAFIVSSSLKFFSSSTSNVLKVGDILSHTRIFTSEDVLEYSKVSHDSNPLHFD 60
           ML RG+FS  I SSSLK FSSSTSNVLKVGDILS+ RIFTSEDVLEYSKVSHDSNPLHFD
Sbjct: 1   MLSRGLFSTHI-SSSLKCFSSSTSNVLKVGDILSYNRIFTSEDVLEYSKVSHDSNPLHFD 60

Query: 61  SALARRAGYEDRLVHGMLVASMFPHIISSHFPGAIYVSQSLNFKLPVYVGEQIIGQVEAV 120
             LARRAG+   LVHG+LVASMFPHIISSH+PGAIYVSQSLNFKLPVYVGE+I+GQVEA+
Sbjct: 61  PELARRAGFNGCLVHGLLVASMFPHIISSHYPGAIYVSQSLNFKLPVYVGEKIVGQVEAI 120

Query: 121 NLKENKKRYLAKFRTLCLRNGGDLVLEGEAMAILPSDFHPTD 163
            L+ENKKRYLAKF+T CLRNG +LVLEGEA AILP+ FH T+
Sbjct: 121 ELRENKKRYLAKFKTKCLRNGDELVLEGEARAILPAYFHSTN 161

[Close]

BLAST of MS005049 vs. ExPASy TrEMBL
Match: A0A1S3CLU3 ((R)-specific enoyl-CoA hydratase OS=Cucumis melo OX=3656 GN=LOC103501925 PE=4 SV=1)

HSP 1 Score: 258.8 bits (660), Expect = 1.5e-65
Identity = 132/162 (81.48%), Postives = 145/162 (89.51%), Query Frame = 0


Query: 1   MLRRGVFSAFIVSSSLKFFSSSTSNVLKVGDILSHTRIFTSEDVLEYSKVSHDSNPLHFD 60
           ML RG+FS  I SSSLK FSSSTSNVLKVGDILS+ RIFTSEDVLEYSKVSHDSNPLHFD
Sbjct: 45  MLSRGLFSTHI-SSSLKCFSSSTSNVLKVGDILSYNRIFTSEDVLEYSKVSHDSNPLHFD 104

Query: 61  SALARRAGYEDRLVHGMLVASMFPHIISSHFPGAIYVSQSLNFKLPVYVGEQIIGQVEAV 120
             LARRAG+   LVHG+LVASMFPHIISSH+PGAIYVSQSLNFKLPVYVGE+I+GQVEA+
Sbjct: 105 PELARRAGFNGCLVHGLLVASMFPHIISSHYPGAIYVSQSLNFKLPVYVGEKIVGQVEAI 164

Query: 121 NLKENKKRYLAKFRTLCLRNGGDLVLEGEAMAILPSDFHPTD 163
            L+ENKKRYLAKF+T CLRNG +LVLEGEA AILP+ FH T+
Sbjct: 165 ELRENKKRYLAKFKTKCLRNGDELVLEGEARAILPAYFHSTN 205

[Close]

BLAST of MS005049 vs. TAIR 10
Match: AT5G60335.1 (Thioesterase superfamily protein )

HSP 1 Score: 179.9 bits (455), Expect = 1.7e-45
Identity = 89/158 (56.33%), Postives = 124/158 (78.48%), Query Frame = 0


Query: 1   MLRRGVFSAFIVSSSLKFFSS-STSNVLKVGDILSHTRIFTSEDVLEYSKVSHDSNPLHF 60
           ML + VF   ++   L+ FSS ++  +LKVGD+L  TR+F+SED+  Y++VSHD NPLHF
Sbjct: 1   MLAKTVFPRGLL--VLRSFSSVASKTLLKVGDVLRETRVFSSEDIKAYAEVSHDWNPLHF 60

Query: 61  DSALARRAGYEDRLVHGMLVASMFPHIISSHFPGAIYVSQSLNFKLPVYVGEQIIGQVEA 120
           D   AR+AG+E+RLVHGMLV+SMFP IIS+HFPGA+YVSQSL+F+ PVY+G++I+G V+A
Sbjct: 61  DPESARKAGFENRLVHGMLVSSMFPRIISAHFPGAVYVSQSLHFRSPVYIGDEILGLVQA 120

Query: 121 VNLKENKKRYLAKFRTLCLRNGGDL-VLEGEAMAILPS 157
           + L+E K +Y+ KF T C +N  +L V++GEA AILP+
Sbjct: 121 IALRETKNKYIVKFSTKCFKNHNELVVIDGEATAILPN 156

The following BLAST results are available for this feature:

BLAST of MS005049 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022140510.1	3.3e-83	99.39	uncharacterized protein LOC111011158 [Momordica charantia]	[more]
XP_038901209.1	5.6e-67	82.72	(R)-specific enoyl-CoA hydratase [Benincasa hispida]	[more]
XP_022985629.1	2.8e-66	82.72	uncharacterized protein LOC111483634 [Cucurbita maxima]	[more]
XP_023512018.1	4.7e-66	82.10	uncharacterized protein LOC111776855 [Cucurbita pepo subsp. pepo]	[more]
KAG7010611.1	8.0e-66	82.10	(R)-specific enoyl-CoA hydratase, partial [Cucurbita argyrosperma subsp. argyros...	[more]

BLAST of MS005049 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O32472	5.3e-20	39.69	(R)-specific enoyl-CoA hydratase OS=Aeromonas caviae OX=648 GN=phaJ PE=1 SV=1	[more]
Q2RQ36	6.7e-15	34.09	(R)-specific enoyl-CoA hydratase OS=Rhodospirillum rubrum (strain ATCC 11170 / A...	[more]
C5AZ74	1.7e-13	32.20	3-hydroxybutyryl-CoA dehydratase OS=Methylorubrum extorquens (strain ATCC 14718 ...	[more]
P86397	6.3e-13	29.55	Hydroxyacyl-thioester dehydratase type 2, mitochondrial OS=Homo sapiens OX=9606 ...	[more]
Q5AV07	7.0e-04	30.00	Fatty acid synthase beta subunit aflB OS=Emericella nidulans (strain FGSC A4 / A...	[more]

BLAST of MS005049 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CFA0	1.6e-83	99.39	uncharacterized protein LOC111011158 OS=Momordica charantia OX=3673 GN=LOC111011...	[more]
A0A6J1JDU3	1.3e-66	82.72	uncharacterized protein LOC111483634 OS=Cucurbita maxima OX=3661 GN=LOC111483634...	[more]
A0A0A0KCN3	6.6e-66	80.86	MaoC-like domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G290840 P...	[more]
A0A5A7T147	1.5e-65	81.48	(R)-specific enoyl-CoA hydratase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676...	[more]
A0A1S3CLU3	1.5e-65	81.48	(R)-specific enoyl-CoA hydratase OS=Cucumis melo OX=3656 GN=LOC103501925 PE=4 SV...	[more]

BLAST of MS005049 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G60335.1	1.7e-45	56.33	Thioesterase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002539	MaoC-like dehydratase domain	PFAM	PF01575	MaoC_dehydratas	coord: 38..127 e-value: 5.6E-16 score: 58.2
None	No IPR available	GENE3D	3.10.129.10	Hotdog Thioesterase	coord: 16..158 e-value: 1.0E-34 score: 121.0
None	No IPR available	PANTHER	PTHR43437	HYDROXYACYL-THIOESTER DEHYDRATASE TYPE 2, MITOCHONDRIAL-RELATED	coord: 11..156
None	No IPR available	PANTHER	PTHR43437:SF3	HYDROXYACYL-THIOESTER DEHYDRATASE TYPE 2, MITOCHONDRIAL-RELATED	coord: 11..156
None	No IPR available	CDD	cd03449	R_hydratase	coord: 27..154 e-value: 2.66056E-44 score: 140.373
IPR029069	HotDog domain superfamily	SUPERFAMILY	54637	Thioesterase/thiol ester dehydrase-isomerase	coord: 27..156

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS005049.1	MS005049.1	mRNA