MS004943 (gene) Bitter gourd (TR) v1

Overview


Name	MS004943
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Thymidine kinase
Location	scaffold176: 1601499 .. 1602552 (+)
RNA-Seq Expression	MS004943
Synteny	MS004943

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGACTCTTTTAGGAGCTCGTTGTCGTTGACCACCACCGACGGCGGCACCGTGAGAGCAGGATCATATCCCCGCGTCGCCGGCGAGGTCCACGTAATCATCGGACCCATGTTCGCCGGGAAAACCACCGCGCTCCTCCGCCGGATAAAATCCGAGAGCAACAGTGGCAGGTAAAGAAAATTGGGGTTTCCGGTGATGAGTTGACTCAGTCGATGCACAATTCGAACTTTTTTGACTTGAATTAGATTTCTATATCGTTCCGTGGGTGAATTTCTGTGTTTAATTCGTCGTGCCATGAGCAGGAATGTGGTGATGATCAAGTCAAGCAAGGACACGAGGTACGCAATTGATTCGGTAGTAACACACGATGGGGCCAAATTTCCATGCTGGGCACTGCCAGATCTTTCGTCTTTCCGTCAGAAATTTGGAGAAGATGCTTACGATAAGGTATCTTTGGCACTATTGTTGGTTTGACAATTTTCTCTTCTAACCCATCTTCTTTCTCACTCCTCAAATAATACTGGCAGCTGGATGTGATTGGTGTTGATGAAGCTCAATTTTTTGACGATCTTTATGATTTTTGCTGCAATGTTGCTGACAAAGATGGCAAAATTGTTATAGTTGCTGGCCTTGATGGAGATTACCTAAGGTACAGTTCAATATTTACATATGGGCCGTTCCCAGATGCAAAACAATGGATCTTTCCAATATAATTGTGCTTGTTTTGCATAATTTTTGGGATCCTTTGCTGGTATTTGAAATCCCGTTTGTAATTGGCAGGAGGAGCTTTGGTTCAGTTCTTGATGTTGTACCTCTGGCTGACACAGTGACAAAGTTGACAGCTAGATGTGAACTTTGTGGCAAAAGAGCCTTCTTTACCTTGAGGAAGACAGAGGAGACAAGGACCGAGCTTATTGCTGGGGCCGATGTCTATATGCCAGTGTGCCGCCAGCACTATGCCAATGGTCAACAAGTGATACAAGCTTCCAAGAATGTCCTGGGATCTCGTACTGTCACAACTGGCTCAAATCTAGAGGCACTTGCTTCACTTAAT

mRNA sequence

ATGGACTCTTTTAGGAGCTCGTTGTCGTTGACCACCACCGACGGCGGCACCGTGAGAGCAGGATCATATCCCCGCGTCGCCGGCGAGGTCCACGTAATCATCGGACCCATGTTCGCCGGGAAAACCACCGCGCTCCTCCGCCGGATAAAATCCGAGAGCAACAGTGGCAGCAGGAATGTGGTGATGATCAAGTCAAGCAAGGACACGAGGTACGCAATTGATTCGGTAGTAACACACGATGGGGCCAAATTTCCATGCTGGGCACTGCCAGATCTTTCGTCTTTCCGTCAGAAATTTGGAGAAGATGCTTACGATAAGCTGGATGTGATTGGTGTTGATGAAGCTCAATTTTTTGACGATCTTTATGATTTTTGCTGCAATGTTGCTGACAAAGATGGCAAAATTGTTATAGTTGCTGGCCTTGATGGAGATTACCTAAGGAGGAGCTTTGGTTCAGTTCTTGATGTTGTACCTCTGGCTGACACAGTGACAAAGTTGACAGCTAGATGTGAACTTTGTGGCAAAAGAGCCTTCTTTACCTTGAGGAAGACAGAGGAGACAAGGACCGAGCTTATTGCTGGGGCCGATGTCTATATGCCAGTGTGCCGCCAGCACTATGCCAATGGTCAACAAGTGATACAAGCTTCCAAGAATGTCCTGGGATCTCGTACTGTCACAACTGGCTCAAATCTAGAGGCACTTGCTTCACTTAAT

Coding sequence (CDS)

ATGGACTCTTTTAGGAGCTCGTTGTCGTTGACCACCACCGACGGCGGCACCGTGAGAGCAGGATCATATCCCCGCGTCGCCGGCGAGGTCCACGTAATCATCGGACCCATGTTCGCCGGGAAAACCACCGCGCTCCTCCGCCGGATAAAATCCGAGAGCAACAGTGGCAGCAGGAATGTGGTGATGATCAAGTCAAGCAAGGACACGAGGTACGCAATTGATTCGGTAGTAACACACGATGGGGCCAAATTTCCATGCTGGGCACTGCCAGATCTTTCGTCTTTCCGTCAGAAATTTGGAGAAGATGCTTACGATAAGCTGGATGTGATTGGTGTTGATGAAGCTCAATTTTTTGACGATCTTTATGATTTTTGCTGCAATGTTGCTGACAAAGATGGCAAAATTGTTATAGTTGCTGGCCTTGATGGAGATTACCTAAGGAGGAGCTTTGGTTCAGTTCTTGATGTTGTACCTCTGGCTGACACAGTGACAAAGTTGACAGCTAGATGTGAACTTTGTGGCAAAAGAGCCTTCTTTACCTTGAGGAAGACAGAGGAGACAAGGACCGAGCTTATTGCTGGGGCCGATGTCTATATGCCAGTGTGCCGCCAGCACTATGCCAATGGTCAACAAGTGATACAAGCTTCCAAGAATGTCCTGGGATCTCGTACTGTCACAACTGGCTCAAATCTAGAGGCACTTGCTTCACTTAAT

Protein sequence

MDSFRSSLSLTTTDGGTVRAGSYPRVAGEVHVIIGPMFAGKTTALLRRIKSESNSGSRNVVMIKSSKDTRYAIDSVVTHDGAKFPCWALPDLSSFRQKFGEDAYDKLDVIGVDEAQFFDDLYDFCCNVADKDGKIVIVAGLDGDYLRRSFGSVLDVVPLADTVTKLTARCELCGKRAFFTLRKTEETRTELIAGADVYMPVCRQHYANGQQVIQASKNVLGSRTVTTGSNLEALASLN

Homology

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BLAST of MS004943 vs. NCBI nr
Match: XP_022140502.1 (thymidine kinase [Momordica charantia])

HSP 1 Score: 467.6 bits (1202), Expect = 6.3e-128
Identity = 237/238 (99.58%), Postives = 237/238 (99.58%), Query Frame = 0


Query: 1   MDSFRSSLSLTTTDGGTVRAGSYPRVAGEVHVIIGPMFAGKTTALLRRIKSESNSGSRNV 60
           MDSFRSSLSLTTTDGGTVRAGSYPRVAGEVHVIIGPMFAGKTTALLRRIKSESNSG RNV
Sbjct: 1   MDSFRSSLSLTTTDGGTVRAGSYPRVAGEVHVIIGPMFAGKTTALLRRIKSESNSG-RNV 60

Query: 61  VMIKSSKDTRYAIDSVVTHDGAKFPCWALPDLSSFRQKFGEDAYDKLDVIGVDEAQFFDD 120
           VMIKSSKDTRYAIDSVVTHDGAKFPCWALPDLSSFRQKFGEDAYDKLDVIGVDEAQFFDD
Sbjct: 61  VMIKSSKDTRYAIDSVVTHDGAKFPCWALPDLSSFRQKFGEDAYDKLDVIGVDEAQFFDD 120

Query: 121 LYDFCCNVADKDGKIVIVAGLDGDYLRRSFGSVLDVVPLADTVTKLTARCELCGKRAFFT 180
           LYDFCCNVADKDGKIVIVAGLDGDYLRRSFGSVLDVVPLADTVTKLTARCELCGKRAFFT
Sbjct: 121 LYDFCCNVADKDGKIVIVAGLDGDYLRRSFGSVLDVVPLADTVTKLTARCELCGKRAFFT 180

Query: 181 LRKTEETRTELIAGADVYMPVCRQHYANGQQVIQASKNVLGSRTVTTGSNLEALASLN 239
           LRKTEETRTELIAGADVYMPVCRQHYANGQQVIQASKNVLGSRTVTTGSNLEALASLN
Sbjct: 181 LRKTEETRTELIAGADVYMPVCRQHYANGQQVIQASKNVLGSRTVTTGSNLEALASLN 237

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BLAST of MS004943 vs. NCBI nr
Match: XP_023513380.1 (thymidine kinase a [Cucurbita pepo subsp. pepo])

HSP 1 Score: 424.5 bits (1090), Expect = 6.1e-115
Identity = 213/237 (89.87%), Postives = 225/237 (94.94%), Query Frame = 0


Query: 1   MDSFRSSLSLTTTDGGTVRAGSYPRVAGEVHVIIGPMFAGKTTALLRRIKSESNSGSRNV 60
           M+S R+S SLT+TDGG V AGS  RVAGEVHVIIGPMFAGKTTALLRRIK+ES+SG RNV
Sbjct: 1   MESLRNSFSLTSTDGGAVSAGSPHRVAGEVHVIIGPMFAGKTTALLRRIKAESSSG-RNV 60

Query: 61  VMIKSSKDTRYAIDSVVTHDGAKFPCWALPDLSSFRQKFGEDAYDKLDVIGVDEAQFFDD 120
           VMIKSSKDTRYAIDSVVTHDGAKFPCWALPDLSSFRQKFGEDAY+KLDVIGVDEAQFFDD
Sbjct: 61  VMIKSSKDTRYAIDSVVTHDGAKFPCWALPDLSSFRQKFGEDAYNKLDVIGVDEAQFFDD 120

Query: 121 LYDFCCNVADKDGKIVIVAGLDGDYLRRSFGSVLDVVPLADTVTKLTARCELCGKRAFFT 180
           LYDFCC+VADKDGKI+IVAGLDGDYLRR FGSVLDVVPLADTVTKLTARCELCGKRAFFT
Sbjct: 121 LYDFCCDVADKDGKIIIVAGLDGDYLRRRFGSVLDVVPLADTVTKLTARCELCGKRAFFT 180

Query: 181 LRKTEETRTELIAGADVYMPVCRQHYANGQQVIQASKNVLGSRTVTTGSNLEALASL 238
           LRKTEETRTELIAGADVYMPVCR HYANGQ+VIQ+SKN+LGSR + TGSNLEAL SL
Sbjct: 181 LRKTEETRTELIAGADVYMPVCRHHYANGQEVIQSSKNILGSRALRTGSNLEALPSL 236

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BLAST of MS004943 vs. NCBI nr
Match: KAG7010772.1 (Thymidine kinase a [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 421.8 bits (1083), Expect = 4.0e-114
Identity = 212/237 (89.45%), Postives = 224/237 (94.51%), Query Frame = 0


Query: 1   MDSFRSSLSLTTTDGGTVRAGSYPRVAGEVHVIIGPMFAGKTTALLRRIKSESNSGSRNV 60
           M+S R+S SLT+TDGG V AGS  RVAGEVHVIIGPMFAGKTTALLRRIK+ES+SG RNV
Sbjct: 1   MESLRNSFSLTSTDGGAVSAGSPHRVAGEVHVIIGPMFAGKTTALLRRIKAESSSG-RNV 60

Query: 61  VMIKSSKDTRYAIDSVVTHDGAKFPCWALPDLSSFRQKFGEDAYDKLDVIGVDEAQFFDD 120
           VMIKSSKDTRYAIDSVVTHDGAKFPCWALPDLSSFRQKFGEDAY+KLDVIGVDEAQFFDD
Sbjct: 61  VMIKSSKDTRYAIDSVVTHDGAKFPCWALPDLSSFRQKFGEDAYNKLDVIGVDEAQFFDD 120

Query: 121 LYDFCCNVADKDGKIVIVAGLDGDYLRRSFGSVLDVVPLADTVTKLTARCELCGKRAFFT 180
           LYDFCC+VADKD KI+IVAGLDGDYLRR FGSVLDVVPLADTVTKLTARCELCGKRAFFT
Sbjct: 121 LYDFCCDVADKDDKIIIVAGLDGDYLRRRFGSVLDVVPLADTVTKLTARCELCGKRAFFT 180

Query: 181 LRKTEETRTELIAGADVYMPVCRQHYANGQQVIQASKNVLGSRTVTTGSNLEALASL 238
           LRKTEETRTELIAGADVYMPVCR HYANGQ+VIQ+SKN+LGSR + TGSNLEAL SL
Sbjct: 181 LRKTEETRTELIAGADVYMPVCRHHYANGQEVIQSSKNILGSRALRTGSNLEALPSL 236

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BLAST of MS004943 vs. NCBI nr
Match: XP_038901065.1 (thymidine kinase isoform X1 [Benincasa hispida])

HSP 1 Score: 421.0 bits (1081), Expect = 6.8e-114
Identity = 212/237 (89.45%), Postives = 224/237 (94.51%), Query Frame = 0


Query: 1   MDSFRSSLSLTTTDGGTVRAGSYPRVAGEVHVIIGPMFAGKTTALLRRIKSESNSGSRNV 60
           M+S RSS SLT+++   VRA S   +AGEVHVIIGPMFAGKTTALLRRIKSESNSG RNV
Sbjct: 1   MESCRSSFSLTSSNDAVVRAESSHCLAGEVHVIIGPMFAGKTTALLRRIKSESNSG-RNV 60

Query: 61  VMIKSSKDTRYAIDSVVTHDGAKFPCWALPDLSSFRQKFGEDAYDKLDVIGVDEAQFFDD 120
           VMIKSSKDTRYAIDSVVTHDGAKFPCWALPDLSSFRQKFGEDAY+KLDVIGVDEAQFFDD
Sbjct: 61  VMIKSSKDTRYAIDSVVTHDGAKFPCWALPDLSSFRQKFGEDAYNKLDVIGVDEAQFFDD 120

Query: 121 LYDFCCNVADKDGKIVIVAGLDGDYLRRSFGSVLDVVPLADTVTKLTARCELCGKRAFFT 180
           LYDFCCNVADKDGKI++VAGLDGDYLRRSFGSVLDV+PLADTVTKLTARCELCGKRAFFT
Sbjct: 121 LYDFCCNVADKDGKIIVVAGLDGDYLRRSFGSVLDVIPLADTVTKLTARCELCGKRAFFT 180

Query: 181 LRKTEETRTELIAGADVYMPVCRQHYANGQQVIQASKNVLGSRTVTTGSNLEALASL 238
           LRKTEET TELIAGADVYMPVCRQHYANGQ+VIQ+SKNVLGSRT+ TGSNLEAL SL
Sbjct: 181 LRKTEETETELIAGADVYMPVCRQHYANGQEVIQSSKNVLGSRTIRTGSNLEALPSL 236

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BLAST of MS004943 vs. NCBI nr
Match: XP_022943895.1 (thymidine kinase a [Cucurbita moschata])

HSP 1 Score: 419.9 bits (1078), Expect = 1.5e-113
Identity = 211/237 (89.03%), Postives = 223/237 (94.09%), Query Frame = 0


Query: 1   MDSFRSSLSLTTTDGGTVRAGSYPRVAGEVHVIIGPMFAGKTTALLRRIKSESNSGSRNV 60
           M+S R+S SL +TDGG V AGS  RVAGEVHVIIGPMFAGKTTALLRRIK+ES+SG RNV
Sbjct: 1   MESLRNSFSLISTDGGAVSAGSPHRVAGEVHVIIGPMFAGKTTALLRRIKAESSSG-RNV 60

Query: 61  VMIKSSKDTRYAIDSVVTHDGAKFPCWALPDLSSFRQKFGEDAYDKLDVIGVDEAQFFDD 120
           VMIKSSKDTRYAIDSVVTHDGAKFPCWALPDLSSFRQKFGEDAY+KLDVIGVDEAQFFDD
Sbjct: 61  VMIKSSKDTRYAIDSVVTHDGAKFPCWALPDLSSFRQKFGEDAYNKLDVIGVDEAQFFDD 120

Query: 121 LYDFCCNVADKDGKIVIVAGLDGDYLRRSFGSVLDVVPLADTVTKLTARCELCGKRAFFT 180
           LYDFCC+VADKD KI+IVAGLDGDYLRR FGSVLDVVPLADTVTKLTARCELCGKRAFFT
Sbjct: 121 LYDFCCDVADKDSKIIIVAGLDGDYLRRRFGSVLDVVPLADTVTKLTARCELCGKRAFFT 180

Query: 181 LRKTEETRTELIAGADVYMPVCRQHYANGQQVIQASKNVLGSRTVTTGSNLEALASL 238
           LRKTEETRTELIAGADVYMPVCR HYANGQ+VIQ+SKN+LGSR + TGSNLEAL SL
Sbjct: 181 LRKTEETRTELIAGADVYMPVCRHHYANGQEVIQSSKNILGSRALRTGSNLEALPSL 236

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BLAST of MS004943 vs. ExPASy Swiss-Prot
Match: O81263 (Thymidine kinase OS=Oryza sativa subsp. japonica OX=39947 GN=TK PE=2 SV=2)

HSP 1 Score: 324.3 bits (830), Expect = 1.1e-87
Identity = 150/206 (72.82%), Postives = 176/206 (85.44%), Query Frame = 0


Query: 15  GGTVRAGSYPRVAGEVHVIIGPMFAGKTTALLRRIKSESNSGSRNVVMIKSSKDTRYAID 74
           GG     + P   GE+HVI+GPMFAGKTTALLRR++ E+ +GSRNV +IKS KD RY +D
Sbjct: 54  GGGSAMEAQPSYPGEIHVIVGPMFAGKTTALLRRVQVEAGTGSRNVALIKSDKDNRYGLD 113

Query: 75  SVVTHDGAKFPCWALPDLSSFRQKFGEDAYDKLDVIGVDEAQFFDDLYDFCCNVADKDGK 134
           SVVTHDG K PCWALP+LSSF+ K G +AYDK+DVIG+DEAQFFDDL+DFCC  AD+DGK
Sbjct: 114 SVVTHDGTKMPCWALPELSSFQDKLGTEAYDKVDVIGIDEAQFFDDLHDFCCKAADRDGK 173

Query: 135 IVIVAGLDGDYLRRSFGSVLDVVPLADTVTKLTARCELCGKRAFFTLRKTEETRTELIAG 194
           IV+VAGLDGDY R  FGSVLD++PLAD+VTKLTARCELCG+RAFFTLRKT ET+TELI G
Sbjct: 174 IVVVAGLDGDYKRNKFGSVLDIIPLADSVTKLTARCELCGRRAFFTLRKTRETKTELIGG 233

Query: 195 ADVYMPVCRQHYANGQQVIQASKNVL 221
           ADVYMPVCRQHY +GQ VI+A++ VL
Sbjct: 234 ADVYMPVCRQHYLDGQIVIEATRIVL 259

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BLAST of MS004943 vs. ExPASy Swiss-Prot
Match: Q9S750 (Thymidine kinase a OS=Arabidopsis thaliana OX=3702 GN=TK1A PE=1 SV=1)

HSP 1 Score: 307.8 bits (787), Expect = 1.1e-82
Identity = 154/223 (69.06%), Postives = 181/223 (81.17%), Query Frame = 0


Query: 1   MDSFRSSLSLTTTDG---GTVRAGSYPRVAGEVHVIIGPMFAGKTTALLRRIKSESNSGS 60
           M + ++S  + T D    G   +    R +G VHVI+GPMF+GK+T+LLRRIKSE + G 
Sbjct: 1   MATLKASFLIKTLDSDVTGDFLSDLERRGSGAVHVIMGPMFSGKSTSLLRRIKSEISDG- 60

Query: 61  RNVVMIKSSKDTRYAIDSVVTHDGAKFPCWALPDLSSFRQKFGEDAYDKLDVIGVDEAQF 120
           R+V M+KSSKDTRYA DSVVTHDG  FPCWALPDL SF +KFG DAY+KLDVIG+DEAQF
Sbjct: 61  RSVAMLKSSKDTRYAKDSVVTHDGIGFPCWALPDLMSFPEKFGLDAYNKLDVIGIDEAQF 120

Query: 121 FDDLYDFCCNVADKDGKIVIVAGLDGDYLRRSFGSVLDVVPLADTVTKLTARCELCGKRA 180
           F DLY+FCC VAD DGKIVIVAGLDGDYLRRSFG+VLD++P+AD+VTKLTARCE+CG +A
Sbjct: 121 FGDLYEFCCKVADDDGKIVIVAGLDGDYLRRSFGAVLDIIPIADSVTKLTARCEVCGHKA 180

Query: 181 FFTLRKTEETRTELIAGADVYMPVCRQHYANGQQVIQASKNVL 221
           FFTLRK  +TRTELI GADVYMPVCR+HY     VI+ASK VL
Sbjct: 181 FFTLRKNCDTRTELIGGADVYMPVCRKHYITNHIVIKASKKVL 222

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BLAST of MS004943 vs. ExPASy Swiss-Prot
Match: F4KBF5 (Thymidine kinase b OS=Arabidopsis thaliana OX=3702 GN=TK1B PE=1 SV=1)

HSP 1 Score: 287.0 bits (733), Expect = 2.0e-76
Identity = 132/196 (67.35%), Postives = 166/196 (84.69%), Query Frame = 0


Query: 28  GEVHVIIGPMFAGKTTALLRRIKSESNSGSRNVVMIKSSKDTRYAIDSVVTHDGAKFPCW 87
           GE+HV++GPMF+GKTT LLRRI +E  +G R + +IKS+KDTRY  +S+VTHDG K+PCW
Sbjct: 77  GEIHVVVGPMFSGKTTTLLRRILAERETGKR-IAIIKSNKDTRYCTESIVTHDGEKYPCW 136

Query: 88  ALPDLSSFRQKFGEDAYD-KLDVIGVDEAQFFDDLYDFCCNVADKDGKIVIVAGLDGDYL 147
           +LPDLSSF+++FG D Y+ +LDVIG+DEAQFF DLY+FC   ADK+GK VIVAGLDGD++
Sbjct: 137 SLPDLSSFKERFGFDDYENRLDVIGIDEAQFFGDLYEFCREAADKEGKTVIVAGLDGDFM 196

Query: 148 RRSFGSVLDVVPLADTVTKLTARCELCGKRAFFTLRKTEETRTELIAGADVYMPVCRQHY 207
           RR FGSVLD++P+ADTVTKLT+RCE+CGKRA FT+RKTEE  TELI GA+VYMPVCR HY
Sbjct: 197 RRRFGSVLDLIPIADTVTKLTSRCEVCGKRALFTMRKTEEKETELIGGAEVYMPVCRSHY 256

Query: 208 ANGQQVIQASKNVLGS 223
             GQ V++ ++ VL S
Sbjct: 257 VCGQNVLETARAVLDS 271

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BLAST of MS004943 vs. ExPASy Swiss-Prot
Match: P23335 (Thymidine kinase OS=Swinepox virus (strain Kasza) OX=10277 GN=TK PE=3 SV=1)

HSP 1 Score: 149.8 bits (377), Expect = 3.8e-35
Identity = 77/179 (43.02%), Postives = 114/179 (63.69%), Query Frame = 0


Query: 28  GEVHVIIGPMFAGKTTALLRRIKSESNSGSRNVVMIKSSKDTRYAIDSVVTHDGAKFPCW 87
           G +H+I+GPMF+GK+T L+R + +     + N  +IK SKD RY  D+V THD       
Sbjct: 4   GFIHLILGPMFSGKSTELIRLV-NRYQIATYNCRVIKYSKDNRYGNDAVYTHDKCYISAV 63

Query: 88  ALPDLSSFRQKFGEDAYDKLDVIGVDEAQFFDDLYDFCCNVADKDGKIVIVAGLDGDYLR 147
           +   L   +     D  D +D++G+DE QFF+D+ +FC  +A+K GKIVIVA LDG Y R
Sbjct: 64  STDSLFDIK-----DTLDDVDIVGIDEGQFFNDIVEFCEYIANK-GKIVIVAALDGTYER 123

Query: 148 RSFGSVLDVVPLADTVTKLTARCELCGKRAFFTLRKTEETRTELIAGADVYMPVCRQHY 207
           + FG++L+++PL++ VTKL A C +C + A F+ R ++E   ELI G + Y+ VCR  Y
Sbjct: 124 KPFGNILNLIPLSEKVTKLNAICMICHRDASFSKRLSDEKEIELIGGKEKYLSVCRSCY 175

[Close]

BLAST of MS004943 vs. ExPASy Swiss-Prot
Match: Q27564 (Thymidine kinase 1 OS=Dictyostelium discoideum OX=44689 GN=thyB PE=1 SV=1)

HSP 1 Score: 143.7 bits (361), Expect = 2.7e-33
Identity = 88/200 (44.00%), Postives = 120/200 (60.00%), Query Frame = 0


Query: 25  RVAGEVHVIIGPMFAGKTTALLRRIKSESNSGSRNVVMIKSSKDTRY--AIDS--VVTHD 84
           ++AG++ VI GPMF+GKTT L+RRIK   N  ++  ++IK SKDTRY   ID   +VTHD
Sbjct: 5   QIAGKIQVIFGPMFSGKTTELIRRIK-RFNFANKKCLLIKYSKDTRYNDNIDKSFLVTHD 64

Query: 85  GAK---FPCWALPDLSSFRQKFGEDAYDKLDVIGVDEAQFFDDLYDFCCNVADKDGKIVI 144
                 FPC  L D+    Q +        DVIG+DE QFF D+  F  ++A++ GK VI
Sbjct: 65  KQNYQAFPCSILEDVKEQAQNY--------DVIGIDEGQFFPDVVQFSEDLANQ-GKTVI 124

Query: 145 VAGLDGDYLRRSFGSVLDVVPLADTVTKLTARCELCGKRAFFTLRKTEETRTELIAGADV 204
           +A LDG + R+ F SV+D+V  A+ +TKLTA C +C   A F+ R  E    ELI G D 
Sbjct: 125 IAALDGTFQRKPFQSVIDLVSKAEYITKLTAVCMVCYNEAAFSKRIVESDDIELIGGIDK 184

Query: 205 YMPVCRQHYANGQQVIQASK 218
           Y+ VCR  Y + Q    ++K
Sbjct: 185 YISVCRGCYNSDQNEGNSTK 194

[Close]

BLAST of MS004943 vs. ExPASy TrEMBL
Match: A0A6J1CH59 (Thymidine kinase OS=Momordica charantia OX=3673 GN=LOC111011151 PE=3 SV=1)

HSP 1 Score: 467.6 bits (1202), Expect = 3.1e-128
Identity = 237/238 (99.58%), Postives = 237/238 (99.58%), Query Frame = 0


Query: 1   MDSFRSSLSLTTTDGGTVRAGSYPRVAGEVHVIIGPMFAGKTTALLRRIKSESNSGSRNV 60
           MDSFRSSLSLTTTDGGTVRAGSYPRVAGEVHVIIGPMFAGKTTALLRRIKSESNSG RNV
Sbjct: 1   MDSFRSSLSLTTTDGGTVRAGSYPRVAGEVHVIIGPMFAGKTTALLRRIKSESNSG-RNV 60

Query: 61  VMIKSSKDTRYAIDSVVTHDGAKFPCWALPDLSSFRQKFGEDAYDKLDVIGVDEAQFFDD 120
           VMIKSSKDTRYAIDSVVTHDGAKFPCWALPDLSSFRQKFGEDAYDKLDVIGVDEAQFFDD
Sbjct: 61  VMIKSSKDTRYAIDSVVTHDGAKFPCWALPDLSSFRQKFGEDAYDKLDVIGVDEAQFFDD 120

Query: 121 LYDFCCNVADKDGKIVIVAGLDGDYLRRSFGSVLDVVPLADTVTKLTARCELCGKRAFFT 180
           LYDFCCNVADKDGKIVIVAGLDGDYLRRSFGSVLDVVPLADTVTKLTARCELCGKRAFFT
Sbjct: 121 LYDFCCNVADKDGKIVIVAGLDGDYLRRSFGSVLDVVPLADTVTKLTARCELCGKRAFFT 180

Query: 181 LRKTEETRTELIAGADVYMPVCRQHYANGQQVIQASKNVLGSRTVTTGSNLEALASLN 239
           LRKTEETRTELIAGADVYMPVCRQHYANGQQVIQASKNVLGSRTVTTGSNLEALASLN
Sbjct: 181 LRKTEETRTELIAGADVYMPVCRQHYANGQQVIQASKNVLGSRTVTTGSNLEALASLN 237

[Close]

BLAST of MS004943 vs. ExPASy TrEMBL
Match: A0A6J1FYC7 (Thymidine kinase OS=Cucurbita moschata OX=3662 GN=LOC111448484 PE=3 SV=1)

HSP 1 Score: 419.9 bits (1078), Expect = 7.3e-114
Identity = 211/237 (89.03%), Postives = 223/237 (94.09%), Query Frame = 0


Query: 1   MDSFRSSLSLTTTDGGTVRAGSYPRVAGEVHVIIGPMFAGKTTALLRRIKSESNSGSRNV 60
           M+S R+S SL +TDGG V AGS  RVAGEVHVIIGPMFAGKTTALLRRIK+ES+SG RNV
Sbjct: 1   MESLRNSFSLISTDGGAVSAGSPHRVAGEVHVIIGPMFAGKTTALLRRIKAESSSG-RNV 60

Query: 61  VMIKSSKDTRYAIDSVVTHDGAKFPCWALPDLSSFRQKFGEDAYDKLDVIGVDEAQFFDD 120
           VMIKSSKDTRYAIDSVVTHDGAKFPCWALPDLSSFRQKFGEDAY+KLDVIGVDEAQFFDD
Sbjct: 61  VMIKSSKDTRYAIDSVVTHDGAKFPCWALPDLSSFRQKFGEDAYNKLDVIGVDEAQFFDD 120

Query: 121 LYDFCCNVADKDGKIVIVAGLDGDYLRRSFGSVLDVVPLADTVTKLTARCELCGKRAFFT 180
           LYDFCC+VADKD KI+IVAGLDGDYLRR FGSVLDVVPLADTVTKLTARCELCGKRAFFT
Sbjct: 121 LYDFCCDVADKDSKIIIVAGLDGDYLRRRFGSVLDVVPLADTVTKLTARCELCGKRAFFT 180

Query: 181 LRKTEETRTELIAGADVYMPVCRQHYANGQQVIQASKNVLGSRTVTTGSNLEALASL 238
           LRKTEETRTELIAGADVYMPVCR HYANGQ+VIQ+SKN+LGSR + TGSNLEAL SL
Sbjct: 181 LRKTEETRTELIAGADVYMPVCRHHYANGQEVIQSSKNILGSRALRTGSNLEALPSL 236

[Close]

BLAST of MS004943 vs. ExPASy TrEMBL
Match: A0A6J1JE52 (Thymidine kinase OS=Cucurbita maxima OX=3661 GN=LOC111483705 PE=3 SV=1)

HSP 1 Score: 415.2 bits (1066), Expect = 1.8e-112
Identity = 210/237 (88.61%), Postives = 222/237 (93.67%), Query Frame = 0


Query: 1   MDSFRSSLSLTTTDGGTVRAGSYPRVAGEVHVIIGPMFAGKTTALLRRIKSESNSGSRNV 60
           M+S R+S S   TDGG V AGS  RVAGEVHVIIGPMFAGKTTALLRRIK+ES+SG RNV
Sbjct: 1   MESLRNSFS---TDGGAVSAGSPHRVAGEVHVIIGPMFAGKTTALLRRIKAESSSG-RNV 60

Query: 61  VMIKSSKDTRYAIDSVVTHDGAKFPCWALPDLSSFRQKFGEDAYDKLDVIGVDEAQFFDD 120
           VMIKSSKDTRYA+DSVVTHDGAKFPCWALPDLSSFRQKFGEDAY+KLDVIGVDEAQFFDD
Sbjct: 61  VMIKSSKDTRYAVDSVVTHDGAKFPCWALPDLSSFRQKFGEDAYNKLDVIGVDEAQFFDD 120

Query: 121 LYDFCCNVADKDGKIVIVAGLDGDYLRRSFGSVLDVVPLADTVTKLTARCELCGKRAFFT 180
           LYDFCC+VADKDGKI+IVAGLDGDYLRR FGSVLDVVPLADTVTKLTARCELCGKRAFFT
Sbjct: 121 LYDFCCDVADKDGKIIIVAGLDGDYLRRRFGSVLDVVPLADTVTKLTARCELCGKRAFFT 180

Query: 181 LRKTEETRTELIAGADVYMPVCRQHYANGQQVIQASKNVLGSRTVTTGSNLEALASL 238
           LRKTEETRTELIAGADVYMPVCR HYANGQ+VIQ+SKN+LGSR + TGSNLEAL SL
Sbjct: 181 LRKTEETRTELIAGADVYMPVCRHHYANGQEVIQSSKNILGSRALRTGSNLEALPSL 233

[Close]

BLAST of MS004943 vs. ExPASy TrEMBL
Match: A0A5A7TX27 (Thymidine kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold243G005900 PE=3 SV=1)

HSP 1 Score: 408.3 bits (1048), Expect = 2.2e-110
Identity = 203/237 (85.65%), Postives = 221/237 (93.25%), Query Frame = 0


Query: 1   MDSFRSSLSLTTTDGGTVRAGSYPRVAGEVHVIIGPMFAGKTTALLRRIKSESNSGSRNV 60
           M+S RSSLSLT+++   +R  S  R+ GEVHVIIGPMFAGKTTALLRRIKS SNSG+RNV
Sbjct: 1   MESCRSSLSLTSSNADAIRPESSHRLTGEVHVIIGPMFAGKTTALLRRIKSASNSGNRNV 60

Query: 61  VMIKSSKDTRYAIDSVVTHDGAKFPCWALPDLSSFRQKFGEDAYDKLDVIGVDEAQFFDD 120
           VMIKSSKDTRYA+DSVVTHDGAKFPCWALP LSSFRQKFGEDAY++LDVIGVDEAQFFDD
Sbjct: 61  VMIKSSKDTRYAVDSVVTHDGAKFPCWALPHLSSFRQKFGEDAYNELDVIGVDEAQFFDD 120

Query: 121 LYDFCCNVADKDGKIVIVAGLDGDYLRRSFGSVLDVVPLADTVTKLTARCELCGKRAFFT 180
           LYDFCCNVAD+DGKI++VAGLDGDYLRRSFGSVLDVVPLADTVTKLTARCELCGKRAFFT
Sbjct: 121 LYDFCCNVADRDGKIIVVAGLDGDYLRRSFGSVLDVVPLADTVTKLTARCELCGKRAFFT 180

Query: 181 LRKTEETRTELIAGADVYMPVCRQHYANGQQVIQASKNVLGSRTVTTGSNLEALASL 238
           LRKTEET+TELIAGADVYMPVCR HYA GQ+VIQ+SKNV+GSRT+T  SNLEAL SL
Sbjct: 181 LRKTEETKTELIAGADVYMPVCRHHYATGQEVIQSSKNVMGSRTIT--SNLEALPSL 235

[Close]

BLAST of MS004943 vs. ExPASy TrEMBL
Match: A0A5D3CJB6 (Thymidine kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold177G00640 PE=3 SV=1)

HSP 1 Score: 401.4 bits (1030), Expect = 2.7e-108
Identity = 202/237 (85.23%), Postives = 219/237 (92.41%), Query Frame = 0


Query: 1   MDSFRSSLSLTTTDGGTVRAGSYPRVAGEVHVIIGPMFAGKTTALLRRIKSESNSGSRNV 60
           M+S RSSLSLT+++   +R  S  R+ GEVHVIIGPMFAGKTTALLRRIKS SNSG RNV
Sbjct: 1   MESCRSSLSLTSSNADAIRPESSHRLTGEVHVIIGPMFAGKTTALLRRIKSASNSG-RNV 60

Query: 61  VMIKSSKDTRYAIDSVVTHDGAKFPCWALPDLSSFRQKFGEDAYDKLDVIGVDEAQFFDD 120
           VMIKSSKDTRYA+DSVVTHDGAKFPCWALP LSSFRQKFGEDAY++LDVIGVDEAQFFDD
Sbjct: 61  VMIKSSKDTRYAVDSVVTHDGAKFPCWALPHLSSFRQKFGEDAYNELDVIGVDEAQFFDD 120

Query: 121 LYDFCCNVADKDGKIVIVAGLDGDYLRRSFGSVLDVVPLADTVTKLTARCELCGKRAFFT 180
           LYDFCCNVAD+DGKI++VAGLDGDYLRRSFGSVLDVVPLADTVTKLTARCELCGKRAFFT
Sbjct: 121 LYDFCCNVADRDGKIIVVAGLDGDYLRRSFGSVLDVVPLADTVTKLTARCELCGKRAFFT 180

Query: 181 LRKTEETRTELIAGADVYMPVCRQHYANGQQVIQASKNVLGSRTVTTGSNLEALASL 238
           LRKTEET+TELIAGADVYMPVCR HYA GQ+VIQ+SKNV+GSRT+   SNLEAL SL
Sbjct: 181 LRKTEETKTELIAGADVYMPVCRHHYATGQEVIQSSKNVMGSRTI--ASNLEALPSL 234

[Close]

BLAST of MS004943 vs. TAIR 10
Match: AT3G07800.1 (Thymidine kinase )

HSP 1 Score: 307.8 bits (787), Expect = 7.8e-84
Identity = 154/223 (69.06%), Postives = 181/223 (81.17%), Query Frame = 0


Query: 1   MDSFRSSLSLTTTDG---GTVRAGSYPRVAGEVHVIIGPMFAGKTTALLRRIKSESNSGS 60
           M + ++S  + T D    G   +    R +G VHVI+GPMF+GK+T+LLRRIKSE + G 
Sbjct: 1   MATLKASFLIKTLDSDVTGDFLSDLERRGSGAVHVIMGPMFSGKSTSLLRRIKSEISDG- 60

Query: 61  RNVVMIKSSKDTRYAIDSVVTHDGAKFPCWALPDLSSFRQKFGEDAYDKLDVIGVDEAQF 120
           R+V M+KSSKDTRYA DSVVTHDG  FPCWALPDL SF +KFG DAY+KLDVIG+DEAQF
Sbjct: 61  RSVAMLKSSKDTRYAKDSVVTHDGIGFPCWALPDLMSFPEKFGLDAYNKLDVIGIDEAQF 120

Query: 121 FDDLYDFCCNVADKDGKIVIVAGLDGDYLRRSFGSVLDVVPLADTVTKLTARCELCGKRA 180
           F DLY+FCC VAD DGKIVIVAGLDGDYLRRSFG+VLD++P+AD+VTKLTARCE+CG +A
Sbjct: 121 FGDLYEFCCKVADDDGKIVIVAGLDGDYLRRSFGAVLDIIPIADSVTKLTARCEVCGHKA 180

Query: 181 FFTLRKTEETRTELIAGADVYMPVCRQHYANGQQVIQASKNVL 221
           FFTLRK  +TRTELI GADVYMPVCR+HY     VI+ASK VL
Sbjct: 181 FFTLRKNCDTRTELIGGADVYMPVCRKHYITNHIVIKASKKVL 222

[Close]

BLAST of MS004943 vs. TAIR 10
Match: AT5G23070.1 (Thymidine kinase )

HSP 1 Score: 287.0 bits (733), Expect = 1.4e-77
Identity = 132/196 (67.35%), Postives = 166/196 (84.69%), Query Frame = 0


Query: 28  GEVHVIIGPMFAGKTTALLRRIKSESNSGSRNVVMIKSSKDTRYAIDSVVTHDGAKFPCW 87
           GE+HV++GPMF+GKTT LLRRI +E  +G R + +IKS+KDTRY  +S+VTHDG K+PCW
Sbjct: 77  GEIHVVVGPMFSGKTTTLLRRILAERETGKR-IAIIKSNKDTRYCTESIVTHDGEKYPCW 136

Query: 88  ALPDLSSFRQKFGEDAYD-KLDVIGVDEAQFFDDLYDFCCNVADKDGKIVIVAGLDGDYL 147
           +LPDLSSF+++FG D Y+ +LDVIG+DEAQFF DLY+FC   ADK+GK VIVAGLDGD++
Sbjct: 137 SLPDLSSFKERFGFDDYENRLDVIGIDEAQFFGDLYEFCREAADKEGKTVIVAGLDGDFM 196

Query: 148 RRSFGSVLDVVPLADTVTKLTARCELCGKRAFFTLRKTEETRTELIAGADVYMPVCRQHY 207
           RR FGSVLD++P+ADTVTKLT+RCE+CGKRA FT+RKTEE  TELI GA+VYMPVCR HY
Sbjct: 197 RRRFGSVLDLIPIADTVTKLTSRCEVCGKRALFTMRKTEEKETELIGGAEVYMPVCRSHY 256

Query: 208 ANGQQVIQASKNVLGS 223
             GQ V++ ++ VL S
Sbjct: 257 VCGQNVLETARAVLDS 271

The following BLAST results are available for this feature:

BLAST of MS004943 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022140502.1	6.3e-128	99.58	thymidine kinase [Momordica charantia]	[more]
XP_023513380.1	6.1e-115	89.87	thymidine kinase a [Cucurbita pepo subsp. pepo]	[more]
KAG7010772.1	4.0e-114	89.45	Thymidine kinase a [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_038901065.1	6.8e-114	89.45	thymidine kinase isoform X1 [Benincasa hispida]	[more]
XP_022943895.1	1.5e-113	89.03	thymidine kinase a [Cucurbita moschata]	[more]

BLAST of MS004943 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O81263	1.1e-87	72.82	Thymidine kinase OS=Oryza sativa subsp. japonica OX=39947 GN=TK PE=2 SV=2	[more]
Q9S750	1.1e-82	69.06	Thymidine kinase a OS=Arabidopsis thaliana OX=3702 GN=TK1A PE=1 SV=1	[more]
F4KBF5	2.0e-76	67.35	Thymidine kinase b OS=Arabidopsis thaliana OX=3702 GN=TK1B PE=1 SV=1	[more]
P23335	3.8e-35	43.02	Thymidine kinase OS=Swinepox virus (strain Kasza) OX=10277 GN=TK PE=3 SV=1	[more]
Q27564	2.7e-33	44.00	Thymidine kinase 1 OS=Dictyostelium discoideum OX=44689 GN=thyB PE=1 SV=1	[more]

BLAST of MS004943 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CH59	3.1e-128	99.58	Thymidine kinase OS=Momordica charantia OX=3673 GN=LOC111011151 PE=3 SV=1	[more]
A0A6J1FYC7	7.3e-114	89.03	Thymidine kinase OS=Cucurbita moschata OX=3662 GN=LOC111448484 PE=3 SV=1	[more]
A0A6J1JE52	1.8e-112	88.61	Thymidine kinase OS=Cucurbita maxima OX=3661 GN=LOC111483705 PE=3 SV=1	[more]
A0A5A7TX27	2.2e-110	85.65	Thymidine kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold243G005...	[more]
A0A5D3CJB6	2.7e-108	85.23	Thymidine kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold177G006...	[more]

BLAST of MS004943 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G07800.1	7.8e-84	69.06	Thymidine kinase	[more]
AT5G23070.1	1.4e-77	67.35	Thymidine kinase	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 234..238
None	No IPR available	GENE3D	3.30.60.20		coord: 167..208 e-value: 1.3E-15 score: 59.4
None	No IPR available	PANTHER	PTHR11441:SF8	THYMIDINE KINASE B	coord: 4..228
None	No IPR available	SUPERFAMILY	57716	Glucocorticoid receptor-like (DNA-binding domain)	coord: 167..209
IPR027417	P-loop containing nucleoside triphosphate hydrolase	GENE3D	3.40.50.300		coord: 23..166 e-value: 9.4E-45 score: 153.9
IPR027417	P-loop containing nucleoside triphosphate hydrolase	SUPERFAMILY	52540	P-loop containing nucleoside triphosphate hydrolases	coord: 28..167
IPR001267	Thymidine kinase	PFAM	PF00265	TK	coord: 28..206 e-value: 7.5E-52 score: 175.8
IPR001267	Thymidine kinase	PANTHER	PTHR11441	THYMIDINE KINASE	coord: 4..228
IPR020633	Thymidine kinase, conserved site	PROSITE	PS00603	TK_CELLULAR_TYPE	coord: 193..206

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS004943.1	MS004943.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004797	thymidine kinase activity