MS004925 (gene) Bitter gourd (TR) v1

Overview
NameMS004925
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptiondnaJ homolog subfamily B member 13-like
Locationscaffold176: 1458338 .. 1458772 (-)
RNA-Seq ExpressionMS004925
SyntenyMS004925
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAACGATGAAAACAGAGGAGATTTTGACAATCAACATAAAACCCGGGTGGAAGAAAGGAACAAAGATCACTTTCCCAGAAAAAGGGAACGAGGAGCCTGATGTTATACCATCAGATCTCGTCTTCGTCATCGATGAGAAGCCTCACAGCTTATTCACTCGCGATGGAAACGATTTAATCGTCACCCAGAAGATATCTCTGGTCGAGGCCTTGACAGGGTGCTCTGTTCATCTCACAACCTTGGATGGCAGACATCTCAATTTCCCAATCACTAATGTCATCACTCCAAATTACGAAGAAGTGATTCCAGGTGAAGGAATGCCGCTCCAAAAAGACCCCACAAAGAAAGGGAATTTGAGAATTCATTTCGTCATCAAGTTTCCCACTAGACTCACCGCCGAGCAGAAGGTCGGCATTAGGAAGCTCTTGGGTCCA

mRNA sequence

AAAACGATGAAAACAGAGGAGATTTTGACAATCAACATAAAACCCGGGTGGAAGAAAGGAACAAAGATCACTTTCCCAGAAAAAGGGAACGAGGAGCCTGATGTTATACCATCAGATCTCGTCTTCGTCATCGATGAGAAGCCTCACAGCTTATTCACTCGCGATGGAAACGATTTAATCGTCACCCAGAAGATATCTCTGGTCGAGGCCTTGACAGGGTGCTCTGTTCATCTCACAACCTTGGATGGCAGACATCTCAATTTCCCAATCACTAATGTCATCACTCCAAATTACGAAGAAGTGATTCCAGGTGAAGGAATGCCGCTCCAAAAAGACCCCACAAAGAAAGGGAATTTGAGAATTCATTTCGTCATCAAGTTTCCCACTAGACTCACCGCCGAGCAGAAGGTCGGCATTAGGAAGCTCTTGGGTCCA

Coding sequence (CDS)

AAAACGATGAAAACAGAGGAGATTTTGACAATCAACATAAAACCCGGGTGGAAGAAAGGAACAAAGATCACTTTCCCAGAAAAAGGGAACGAGGAGCCTGATGTTATACCATCAGATCTCGTCTTCGTCATCGATGAGAAGCCTCACAGCTTATTCACTCGCGATGGAAACGATTTAATCGTCACCCAGAAGATATCTCTGGTCGAGGCCTTGACAGGGTGCTCTGTTCATCTCACAACCTTGGATGGCAGACATCTCAATTTCCCAATCACTAATGTCATCACTCCAAATTACGAAGAAGTGATTCCAGGTGAAGGAATGCCGCTCCAAAAAGACCCCACAAAGAAAGGGAATTTGAGAATTCATTTCGTCATCAAGTTTCCCACTAGACTCACCGCCGAGCAGAAGGTCGGCATTAGGAAGCTCTTGGGTCCA

Protein sequence

KTMKTEEILTINIKPGWKKGTKITFPEKGNEEPDVIPSDLVFVIDEKPHSLFTRDGNDLIVTQKISLVEALTGCSVHLTTLDGRHLNFPITNVITPNYEEVIPGEGMPLQKDPTKKGNLRIHFVIKFPTRLTAEQKVGIRKLLGP
Homology
BLAST of MS004925 vs. NCBI nr
Match: XP_022140635.1 (dnaJ homolog subfamily B member 13-like [Momordica charantia])

HSP 1 Score: 297.7 bits (761), Expect = 5.3e-77
Identity = 144/145 (99.31%), Postives = 144/145 (99.31%), Query Frame = 0

Query: 1   KTMKTEEILTINIKPGWKKGTKITFPEKGNEEPDVIPSDLVFVIDEKPHSLFTRDGNDLI 60
           KTMKTEEILTINIKPGWKKGTKITFPEKGNEEPDVIPSDLVFVIDEKPHSLFTRDGNDLI
Sbjct: 276 KTMKTEEILTINIKPGWKKGTKITFPEKGNEEPDVIPSDLVFVIDEKPHSLFTRDGNDLI 335

Query: 61  VTQKISLVEALTGCSVHLTTLDGRHLNFPITNVITPNYEEVIPGEGMPLQKDPTKKGNLR 120
           VTQKISLVEALTGCSVHLTTLDGRHLNFPITNVITPNYEEVIP EGMPLQKDPTKKGNLR
Sbjct: 336 VTQKISLVEALTGCSVHLTTLDGRHLNFPITNVITPNYEEVIPAEGMPLQKDPTKKGNLR 395

Query: 121 IHFVIKFPTRLTAEQKVGIRKLLGP 146
           IHFVIKFPTRLTAEQKVGIRKLLGP
Sbjct: 396 IHFVIKFPTRLTAEQKVGIRKLLGP 420

BLAST of MS004925 vs. NCBI nr
Match: XP_004138602.1 (dnaJ homolog subfamily B member 13 [Cucumis sativus] >KGN63200.1 hypothetical protein Csa_022530 [Cucumis sativus])

HSP 1 Score: 273.9 bits (699), Expect = 8.2e-70
Identity = 132/144 (91.67%), Postives = 139/144 (96.53%), Query Frame = 0

Query: 1   KTMKTEEILTINIKPGWKKGTKITFPEKGNEEPDVIPSDLVFVIDEKPHSLFTRDGNDLI 60
           KTMKTEEILTINIKPGWKKGTKITFPEKGNEEPD+IPSDLVFVIDEKPHS+FTRDGNDLI
Sbjct: 199 KTMKTEEILTINIKPGWKKGTKITFPEKGNEEPDIIPSDLVFVIDEKPHSVFTRDGNDLI 258

Query: 61  VTQKISLVEALTGCSVHLTTLDGRHLNFPITNVITPNYEEVIPGEGMPLQKDPTKKGNLR 120
           VTQKISLVEALTG +VHLTTLDGR+L+FPITNVITPNYEEVIP EGMPLQKDPTKKGNLR
Sbjct: 259 VTQKISLVEALTGYTVHLTTLDGRYLSFPITNVITPNYEEVIPSEGMPLQKDPTKKGNLR 318

Query: 121 IHFVIKFPTRLTAEQKVGIRKLLG 145
           I+F IKFPTRLT EQK GIRKL+G
Sbjct: 319 INFDIKFPTRLTPEQKAGIRKLIG 342

BLAST of MS004925 vs. NCBI nr
Match: XP_038902656.1 (dnaJ protein homolog 1-like [Benincasa hispida])

HSP 1 Score: 271.6 bits (693), Expect = 4.1e-69
Identity = 132/143 (92.31%), Postives = 134/143 (93.71%), Query Frame = 0

Query: 1   KTMKTEEILTINIKPGWKKGTKITFPEKGNEEPDVIPSDLVFVIDEKPHSLFTRDGNDLI 60
           KTMKTEEILTINIKPGWKKGTKITFPEKGNEEPD+IPSDLVFVIDEKPHS+FTRDGNDLI
Sbjct: 200 KTMKTEEILTINIKPGWKKGTKITFPEKGNEEPDIIPSDLVFVIDEKPHSVFTRDGNDLI 259

Query: 61  VTQKISLVEALTGCSVHLTTLDGRHLNFPITNVITPNYEEVIPGEGMPLQKDPTKKGNLR 120
           VTQKISL EALTG SVHLTTLDGRHLNFPITNVITPNYEEVIP EGMPLQKDPTKKGNLR
Sbjct: 260 VTQKISLAEALTGYSVHLTTLDGRHLNFPITNVITPNYEEVIPSEGMPLQKDPTKKGNLR 319

Query: 121 IHFVIKFPTRLTAEQKVGIRKLL 144
           I F IKFP RL  EQK GIRKLL
Sbjct: 320 IKFDIKFPARLAPEQKAGIRKLL 342

BLAST of MS004925 vs. NCBI nr
Match: KAG7010760.1 (DnaJ-like subfamily B member 13 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 270.4 bits (690), Expect = 9.0e-69
Identity = 130/144 (90.28%), Postives = 136/144 (94.44%), Query Frame = 0

Query: 1   KTMKTEEILTINIKPGWKKGTKITFPEKGNEEPDVIPSDLVFVIDEKPHSLFTRDGNDLI 60
           K  KTEEILTINIKPGWKKGTKITFPEKGNEEPD+IPSDLVF+IDEKPHS+FTRDGNDLI
Sbjct: 81  KATKTEEILTINIKPGWKKGTKITFPEKGNEEPDIIPSDLVFLIDEKPHSVFTRDGNDLI 140

Query: 61  VTQKISLVEALTGCSVHLTTLDGRHLNFPITNVITPNYEEVIPGEGMPLQKDPTKKGNLR 120
            TQKISL EALTG +VHLTTLDGRHL+FPITNVITPNYEEVIP EGMPLQKDPTKKGNLR
Sbjct: 141 ATQKISLAEALTGYTVHLTTLDGRHLSFPITNVITPNYEEVIPSEGMPLQKDPTKKGNLR 200

Query: 121 IHFVIKFPTRLTAEQKVGIRKLLG 145
           I+F IKFPTRLTAEQK GIRKLLG
Sbjct: 201 INFDIKFPTRLTAEQKAGIRKLLG 224

BLAST of MS004925 vs. NCBI nr
Match: XP_023512074.1 (dnaJ homolog subfamily B member 13-like [Cucurbita pepo subsp. pepo] >KAG6570917.1 DnaJ-like subfamily B member 13, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 270.4 bits (690), Expect = 9.0e-69
Identity = 130/144 (90.28%), Postives = 136/144 (94.44%), Query Frame = 0

Query: 1   KTMKTEEILTINIKPGWKKGTKITFPEKGNEEPDVIPSDLVFVIDEKPHSLFTRDGNDLI 60
           K  KTEEILTINIKPGWKKGTKITFPEKGNEEPD+IPSDLVF+IDEKPHS+FTRDGNDLI
Sbjct: 205 KATKTEEILTINIKPGWKKGTKITFPEKGNEEPDIIPSDLVFLIDEKPHSVFTRDGNDLI 264

Query: 61  VTQKISLVEALTGCSVHLTTLDGRHLNFPITNVITPNYEEVIPGEGMPLQKDPTKKGNLR 120
            TQKISL EALTG +VHLTTLDGRHL+FPITNVITPNYEEVIP EGMPLQKDPTKKGNLR
Sbjct: 265 ATQKISLAEALTGYTVHLTTLDGRHLSFPITNVITPNYEEVIPSEGMPLQKDPTKKGNLR 324

Query: 121 IHFVIKFPTRLTAEQKVGIRKLLG 145
           I+F IKFPTRLTAEQK GIRKLLG
Sbjct: 325 INFDIKFPTRLTAEQKAGIRKLLG 348

BLAST of MS004925 vs. ExPASy Swiss-Prot
Match: P59910 (DnaJ homolog subfamily B member 13 OS=Homo sapiens OX=9606 GN=DNAJB13 PE=1 SV=1)

HSP 1 Score: 154.5 bits (389), Expect = 9.5e-37
Identity = 69/138 (50.00%), Postives = 104/138 (75.36%), Query Frame = 0

Query: 6   EEILTINIKPGWKKGTKITFPEKGNEEPDVIPSDLVFVIDEKPHSLFTRDGNDLIVTQKI 65
           ++ILTI++KPGW++GT+ITF ++G++ P++IP+D++F++ EK H  F R+ ++L     I
Sbjct: 177 DKILTIDVKPGWRQGTRITFEKEGDQGPNIIPADIIFIVKEKLHPRFRRENDNLFFVNPI 236

Query: 66  SLVEALTGCSVHLTTLDGRHLNFPITNVITPNYEEVIPGEGMPLQKDPTKKGNLRIHFVI 125
            L +ALT C+V + TLD R LN PI ++I P Y + +PGEGMPL +DPTKKG+L I F I
Sbjct: 237 PLGKALTCCTVEVRTLDDRLLNIPINDIIHPKYFKKVPGEGMPLPEDPTKKGDLFIFFDI 296

Query: 126 KFPTRLTAEQKVGIRKLL 144
           +FPTRLT ++K  +R+ L
Sbjct: 297 QFPTRLTPQKKQMLRQAL 314

BLAST of MS004925 vs. ExPASy Swiss-Prot
Match: Q80Y75 (DnaJ homolog subfamily B member 13 OS=Mus musculus OX=10090 GN=Dnajb13 PE=1 SV=1)

HSP 1 Score: 150.2 bits (378), Expect = 1.8e-35
Identity = 65/138 (47.10%), Postives = 105/138 (76.09%), Query Frame = 0

Query: 6   EEILTINIKPGWKKGTKITFPEKGNEEPDVIPSDLVFVIDEKPHSLFTRDGNDLIVTQKI 65
           ++ILTI+++PGW++GT+ITF ++G++ P++IP+D++F++ EK H  F R+ ++L     I
Sbjct: 177 DKILTIDVRPGWRQGTRITFEKEGDQGPNIIPADIIFIVKEKLHPRFRREHDNLFFVYPI 236

Query: 66  SLVEALTGCSVHLTTLDGRHLNFPITNVITPNYEEVIPGEGMPLQKDPTKKGNLRIHFVI 125
            L +ALT C+V + TLD R LN PI +++ P Y +++PGEGMPL ++P+KKG+L I F I
Sbjct: 237 PLGKALTCCTVEVKTLDDRLLNIPINDIVHPKYFKIVPGEGMPLPENPSKKGDLFIFFDI 296

Query: 126 KFPTRLTAEQKVGIRKLL 144
           +FPTRLT ++K  +R+ L
Sbjct: 297 QFPTRLTPQKKQMLRQAL 314

BLAST of MS004925 vs. ExPASy Swiss-Prot
Match: P25685 (DnaJ homolog subfamily B member 1 OS=Homo sapiens OX=9606 GN=DNAJB1 PE=1 SV=4)

HSP 1 Score: 144.8 bits (364), Expect = 7.5e-34
Identity = 67/144 (46.53%), Postives = 102/144 (70.83%), Query Frame = 0

Query: 1   KTMKTEE-ILTINIKPGWKKGTKITFPEKGNEEPDVIPSDLVFVIDEKPHSLFTRDGNDL 60
           K+++ E+ ILTI +K GWK+GTKITFP++G++  + IP+D+VFV+ +KPH++F RDG+D+
Sbjct: 195 KSIRNEDKILTIEVKKGWKEGTKITFPKEGDQTSNNIPADIVFVLKDKPHNIFKRDGSDV 254

Query: 61  IVTQKISLVEALTGCSVHLTTLDGRHLNFPITNVITPNYEEVIPGEGMPLQKDPTKKGNL 120
           I   +ISL EAL GC+V++ TLDGR +     +VI P     +PGEG+PL K P K+G+L
Sbjct: 255 IYPARISLREALCGCTVNVPTLDGRTIPVVFKDVIRPGMRRKVPGEGLPLPKTPEKRGDL 314

Query: 121 RIHFVIKFPTRLTAEQKVGIRKLL 144
            I F + FP R+    +  + ++L
Sbjct: 315 IIEFEVIFPERIPQTSRTVLEQVL 338

BLAST of MS004925 vs. ExPASy Swiss-Prot
Match: Q9QYJ3 (DnaJ homolog subfamily B member 1 OS=Mus musculus OX=10090 GN=Dnajb1 PE=1 SV=3)

HSP 1 Score: 144.8 bits (364), Expect = 7.5e-34
Identity = 67/144 (46.53%), Postives = 102/144 (70.83%), Query Frame = 0

Query: 1   KTMKTEE-ILTINIKPGWKKGTKITFPEKGNEEPDVIPSDLVFVIDEKPHSLFTRDGNDL 60
           K+++ E+ ILTI +K GWK+GTKITFP++G++  + IP+D+VFV+ +KPH++F RDG+D+
Sbjct: 195 KSIRNEDKILTIEVKRGWKEGTKITFPKEGDQTSNNIPADIVFVLKDKPHNIFKRDGSDV 254

Query: 61  IVTQKISLVEALTGCSVHLTTLDGRHLNFPITNVITPNYEEVIPGEGMPLQKDPTKKGNL 120
           I   +ISL EAL GC+V++ TLDGR +     +VI P     +PGEG+PL K P K+G+L
Sbjct: 255 IYPARISLREALCGCTVNVPTLDGRTIPVVFKDVIRPGMRRKVPGEGLPLPKTPEKRGDL 314

Query: 121 RIHFVIKFPTRLTAEQKVGIRKLL 144
            I F + FP R+    +  + ++L
Sbjct: 315 VIEFEVIFPERIPVSSRTILEQVL 338

BLAST of MS004925 vs. ExPASy Swiss-Prot
Match: Q3MI00 (DnaJ homolog subfamily B member 1 OS=Bos taurus OX=9913 GN=DNAJB1 PE=2 SV=3)

HSP 1 Score: 144.4 bits (363), Expect = 9.8e-34
Identity = 67/144 (46.53%), Postives = 102/144 (70.83%), Query Frame = 0

Query: 1   KTMKTEE-ILTINIKPGWKKGTKITFPEKGNEEPDVIPSDLVFVIDEKPHSLFTRDGNDL 60
           K+++ E+ ILTI +K GWK+GTKITFP++G++  + IP+D+VFV+ +KPH++F RDG+D+
Sbjct: 195 KSIRNEDKILTIEVKRGWKEGTKITFPKEGDQTSNNIPADIVFVLKDKPHNIFKRDGSDV 254

Query: 61  IVTQKISLVEALTGCSVHLTTLDGRHLNFPITNVITPNYEEVIPGEGMPLQKDPTKKGNL 120
           I   +ISL EAL GC+V++ TLDGR +     +VI P     +PGEG+PL K P K+G+L
Sbjct: 255 IYPARISLREALCGCTVNVPTLDGRTIPVVFKDVIRPGMRRKVPGEGLPLPKTPEKRGDL 314

Query: 121 RIHFVIKFPTRLTAEQKVGIRKLL 144
            I F + FP R+    +  + ++L
Sbjct: 315 IIEFEVIFPERIPQTSRTVLEQVL 338

BLAST of MS004925 vs. ExPASy TrEMBL
Match: A0A6J1CFM6 (dnaJ homolog subfamily B member 13-like OS=Momordica charantia OX=3673 GN=LOC111011244 PE=4 SV=1)

HSP 1 Score: 297.7 bits (761), Expect = 2.6e-77
Identity = 144/145 (99.31%), Postives = 144/145 (99.31%), Query Frame = 0

Query: 1   KTMKTEEILTINIKPGWKKGTKITFPEKGNEEPDVIPSDLVFVIDEKPHSLFTRDGNDLI 60
           KTMKTEEILTINIKPGWKKGTKITFPEKGNEEPDVIPSDLVFVIDEKPHSLFTRDGNDLI
Sbjct: 276 KTMKTEEILTINIKPGWKKGTKITFPEKGNEEPDVIPSDLVFVIDEKPHSLFTRDGNDLI 335

Query: 61  VTQKISLVEALTGCSVHLTTLDGRHLNFPITNVITPNYEEVIPGEGMPLQKDPTKKGNLR 120
           VTQKISLVEALTGCSVHLTTLDGRHLNFPITNVITPNYEEVIP EGMPLQKDPTKKGNLR
Sbjct: 336 VTQKISLVEALTGCSVHLTTLDGRHLNFPITNVITPNYEEVIPAEGMPLQKDPTKKGNLR 395

Query: 121 IHFVIKFPTRLTAEQKVGIRKLLGP 146
           IHFVIKFPTRLTAEQKVGIRKLLGP
Sbjct: 396 IHFVIKFPTRLTAEQKVGIRKLLGP 420

BLAST of MS004925 vs. ExPASy TrEMBL
Match: A0A0A0LMP6 (J domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G408950 PE=4 SV=1)

HSP 1 Score: 273.9 bits (699), Expect = 4.0e-70
Identity = 132/144 (91.67%), Postives = 139/144 (96.53%), Query Frame = 0

Query: 1   KTMKTEEILTINIKPGWKKGTKITFPEKGNEEPDVIPSDLVFVIDEKPHSLFTRDGNDLI 60
           KTMKTEEILTINIKPGWKKGTKITFPEKGNEEPD+IPSDLVFVIDEKPHS+FTRDGNDLI
Sbjct: 199 KTMKTEEILTINIKPGWKKGTKITFPEKGNEEPDIIPSDLVFVIDEKPHSVFTRDGNDLI 258

Query: 61  VTQKISLVEALTGCSVHLTTLDGRHLNFPITNVITPNYEEVIPGEGMPLQKDPTKKGNLR 120
           VTQKISLVEALTG +VHLTTLDGR+L+FPITNVITPNYEEVIP EGMPLQKDPTKKGNLR
Sbjct: 259 VTQKISLVEALTGYTVHLTTLDGRYLSFPITNVITPNYEEVIPSEGMPLQKDPTKKGNLR 318

Query: 121 IHFVIKFPTRLTAEQKVGIRKLLG 145
           I+F IKFPTRLT EQK GIRKL+G
Sbjct: 319 INFDIKFPTRLTPEQKAGIRKLIG 342

BLAST of MS004925 vs. ExPASy TrEMBL
Match: A0A5D3DA89 (DnaJ-like protein subfamily B member 13-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold604G00090 PE=4 SV=1)

HSP 1 Score: 270.4 bits (690), Expect = 4.4e-69
Identity = 130/144 (90.28%), Postives = 138/144 (95.83%), Query Frame = 0

Query: 1   KTMKTEEILTINIKPGWKKGTKITFPEKGNEEPDVIPSDLVFVIDEKPHSLFTRDGNDLI 60
           +T KTEEILTINIKPGWKKGTKITFPEKGNEEPD+IPSDLVFVIDEKPHS+FTRDGNDLI
Sbjct: 199 RTTKTEEILTINIKPGWKKGTKITFPEKGNEEPDIIPSDLVFVIDEKPHSVFTRDGNDLI 258

Query: 61  VTQKISLVEALTGCSVHLTTLDGRHLNFPITNVITPNYEEVIPGEGMPLQKDPTKKGNLR 120
           VTQKISLVEALTG +VHLTTLDGR+L+FPITNVITPNYEEVIP EGMPLQKDPTKKGNLR
Sbjct: 259 VTQKISLVEALTGYTVHLTTLDGRYLSFPITNVITPNYEEVIPSEGMPLQKDPTKKGNLR 318

Query: 121 IHFVIKFPTRLTAEQKVGIRKLLG 145
           I+F IKFPTRLT EQK GIRK+LG
Sbjct: 319 INFDIKFPTRLTPEQKAGIRKILG 342

BLAST of MS004925 vs. ExPASy TrEMBL
Match: A0A6J1FSM3 (dnaJ homolog subfamily B member 13-like OS=Cucurbita moschata OX=3662 GN=LOC111448104 PE=4 SV=1)

HSP 1 Score: 270.4 bits (690), Expect = 4.4e-69
Identity = 130/144 (90.28%), Postives = 136/144 (94.44%), Query Frame = 0

Query: 1   KTMKTEEILTINIKPGWKKGTKITFPEKGNEEPDVIPSDLVFVIDEKPHSLFTRDGNDLI 60
           K  KTEEILTINIKPGWKKGTKITFPEKGNEEPD+IPSDLVF+IDEKPHS+FTRDGNDLI
Sbjct: 205 KATKTEEILTINIKPGWKKGTKITFPEKGNEEPDIIPSDLVFLIDEKPHSVFTRDGNDLI 264

Query: 61  VTQKISLVEALTGCSVHLTTLDGRHLNFPITNVITPNYEEVIPGEGMPLQKDPTKKGNLR 120
            TQKISL EALTG +VHLTTLDGRHL+FPITNVITPNYEEVIP EGMPLQKDPTKKGNLR
Sbjct: 265 ATQKISLAEALTGYTVHLTTLDGRHLSFPITNVITPNYEEVIPSEGMPLQKDPTKKGNLR 324

Query: 121 IHFVIKFPTRLTAEQKVGIRKLLG 145
           I+F IKFPTRLTAEQK GIRKLLG
Sbjct: 325 INFDIKFPTRLTAEQKAGIRKLLG 348

BLAST of MS004925 vs. ExPASy TrEMBL
Match: A0A1S3C229 (dnaJ homolog subfamily B member 13-like OS=Cucumis melo OX=3656 GN=LOC103496013 PE=4 SV=1)

HSP 1 Score: 270.4 bits (690), Expect = 4.4e-69
Identity = 130/144 (90.28%), Postives = 138/144 (95.83%), Query Frame = 0

Query: 1   KTMKTEEILTINIKPGWKKGTKITFPEKGNEEPDVIPSDLVFVIDEKPHSLFTRDGNDLI 60
           +T KTEEILTINIKPGWKKGTKITFPEKGNEEPD+IPSDLVFVIDEKPHS+FTRDGNDLI
Sbjct: 199 RTTKTEEILTINIKPGWKKGTKITFPEKGNEEPDIIPSDLVFVIDEKPHSVFTRDGNDLI 258

Query: 61  VTQKISLVEALTGCSVHLTTLDGRHLNFPITNVITPNYEEVIPGEGMPLQKDPTKKGNLR 120
           VTQKISLVEALTG +VHLTTLDGR+L+FPITNVITPNYEEVIP EGMPLQKDPTKKGNLR
Sbjct: 259 VTQKISLVEALTGYTVHLTTLDGRYLSFPITNVITPNYEEVIPSEGMPLQKDPTKKGNLR 318

Query: 121 IHFVIKFPTRLTAEQKVGIRKLLG 145
           I+F IKFPTRLT EQK GIRK+LG
Sbjct: 319 INFDIKFPTRLTPEQKAGIRKILG 342

BLAST of MS004925 vs. TAIR 10
Match: AT2G20560.1 (DNAJ heat shock family protein )

HSP 1 Score: 241.5 bits (615), Expect = 4.2e-64
Identity = 113/144 (78.47%), Postives = 130/144 (90.28%), Query Frame = 0

Query: 1   KTMKTEEILTINIKPGWKKGTKITFPEKGNEEPDVIPSDLVFVIDEKPHSLFTRDGNDLI 60
           KTM+ EEILTI++KPGWKKGTKITFPEKGNE+P VIP+DLVF+IDEKPH +FTR+GNDLI
Sbjct: 194 KTMQVEEILTIDVKPGWKKGTKITFPEKGNEQPGVIPADLVFIIDEKPHPVFTREGNDLI 253

Query: 61  VTQKISLVEALTGCSVHLTTLDGRHLNFPITNVITPNYEEVIPGEGMPLQKDPTKKGNLR 120
           VTQKISLVEALTG +V+LTTLDGR L  P+TNV+ P YEEV+P EGMPLQKD TK+GNLR
Sbjct: 254 VTQKISLVEALTGYTVNLTTLDGRRLTIPVTNVVHPEYEEVVPKEGMPLQKDQTKRGNLR 313

Query: 121 IHFVIKFPTRLTAEQKVGIRKLLG 145
           I F IKFPTRLT+EQK G++KLLG
Sbjct: 314 IKFNIKFPTRLTSEQKTGVKKLLG 337

BLAST of MS004925 vs. TAIR 10
Match: AT4G28480.1 (DNAJ heat shock family protein )

HSP 1 Score: 229.6 bits (584), Expect = 1.6e-60
Identity = 108/144 (75.00%), Postives = 122/144 (84.72%), Query Frame = 0

Query: 1   KTMKTEEILTINIKPGWKKGTKITFPEKGNEEPDVIPSDLVFVIDEKPHSLFTRDGNDLI 60
           K M+ EEILTI +KPGWKKGTKITFPEKGNE P VIP+DLVF+IDEKPH +FTR+GNDLI
Sbjct: 205 KAMQVEEILTIGVKPGWKKGTKITFPEKGNEHPGVIPADLVFIIDEKPHPVFTREGNDLI 264

Query: 61  VTQKISLVEALTGCSVHLTTLDGRHLNFPITNVITPNYEEVIPGEGMPLQKDPTKKGNLR 120
           VTQK+SL +ALTG + ++ TLDGR L  PITNVI P YEEV+P EGMPLQKD TKKGNLR
Sbjct: 265 VTQKVSLADALTGYTANIATLDGRTLTIPITNVIHPEYEEVVPKEGMPLQKDQTKKGNLR 324

Query: 121 IHFVIKFPTRLTAEQKVGIRKLLG 145
           I F IKFP RLTAEQK G +KL+G
Sbjct: 325 IKFNIKFPARLTAEQKAGFKKLIG 348

BLAST of MS004925 vs. TAIR 10
Match: AT4G28480.2 (DNAJ heat shock family protein )

HSP 1 Score: 226.1 bits (575), Expect = 1.8e-59
Identity = 106/139 (76.26%), Postives = 119/139 (85.61%), Query Frame = 0

Query: 6   EEILTINIKPGWKKGTKITFPEKGNEEPDVIPSDLVFVIDEKPHSLFTRDGNDLIVTQKI 65
           EEILTI +KPGWKKGTKITFPEKGNE P VIP+DLVF+IDEKPH +FTR+GNDLIVTQK+
Sbjct: 152 EEILTIGVKPGWKKGTKITFPEKGNEHPGVIPADLVFIIDEKPHPVFTREGNDLIVTQKV 211

Query: 66  SLVEALTGCSVHLTTLDGRHLNFPITNVITPNYEEVIPGEGMPLQKDPTKKGNLRIHFVI 125
           SL +ALTG + ++ TLDGR L  PITNVI P YEEV+P EGMPLQKD TKKGNLRI F I
Sbjct: 212 SLADALTGYTANIATLDGRTLTIPITNVIHPEYEEVVPKEGMPLQKDQTKKGNLRIKFNI 271

Query: 126 KFPTRLTAEQKVGIRKLLG 145
           KFP RLTAEQK G +KL+G
Sbjct: 272 KFPARLTAEQKAGFKKLIG 290

BLAST of MS004925 vs. TAIR 10
Match: AT2G20550.1 (HSP40/DnaJ peptide-binding protein )

HSP 1 Score: 218.8 bits (556), Expect = 2.9e-57
Identity = 101/144 (70.14%), Postives = 121/144 (84.03%), Query Frame = 0

Query: 1   KTMKTEEILTINIKPGWKKGTKITFPEKGNEEPDVIPSDLVFVIDEKPHSLFTRDGNDLI 60
           KT + +EILT+++KPGWK GTKITF EKGNE+P VIP+DLVF+IDEKPH +FTR+GNDL+
Sbjct: 141 KTTQVQEILTVDVKPGWKTGTKITFSEKGNEQPGVIPADLVFIIDEKPHPVFTREGNDLV 200

Query: 61  VTQKISLVEALTGCSVHLTTLDGRHLNFPITNVITPNYEEVIPGEGMPLQKDPTKKGNLR 120
           VTQKIS++EA TG +V+LTTLDGR L  P+  VI P Y EV+P EGMPLQKD  KKGNLR
Sbjct: 201 VTQKISVLEAFTGYTVNLTTLDGRRLTIPVNTVIHPEYVEVVPNEGMPLQKDQAKKGNLR 260

Query: 121 IHFVIKFPTRLTAEQKVGIRKLLG 145
           I F IKFPT LT+EQK G++KLLG
Sbjct: 261 IKFNIKFPTTLTSEQKTGLKKLLG 284

BLAST of MS004925 vs. TAIR 10
Match: AT2G20550.2 (HSP40/DnaJ peptide-binding protein )

HSP 1 Score: 218.8 bits (556), Expect = 2.9e-57
Identity = 101/144 (70.14%), Postives = 121/144 (84.03%), Query Frame = 0

Query: 1   KTMKTEEILTINIKPGWKKGTKITFPEKGNEEPDVIPSDLVFVIDEKPHSLFTRDGNDLI 60
           KT + +EILT+++KPGWK GTKITF EKGNE+P VIP+DLVF+IDEKPH +FTR+GNDL+
Sbjct: 141 KTTQVQEILTVDVKPGWKTGTKITFSEKGNEQPGVIPADLVFIIDEKPHPVFTREGNDLV 200

Query: 61  VTQKISLVEALTGCSVHLTTLDGRHLNFPITNVITPNYEEVIPGEGMPLQKDPTKKGNLR 120
           VTQKIS++EA TG +V+LTTLDGR L  P+  VI P Y EV+P EGMPLQKD  KKGNLR
Sbjct: 201 VTQKISVLEAFTGYTVNLTTLDGRRLTIPVNTVIHPEYVEVVPNEGMPLQKDQAKKGNLR 260

Query: 121 IHFVIKFPTRLTAEQKVGIRKLLG 145
           I F IKFPT LT+EQK G++KLLG
Sbjct: 261 IKFNIKFPTTLTSEQKTGLKKLLG 284

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022140635.15.3e-7799.31dnaJ homolog subfamily B member 13-like [Momordica charantia][more]
XP_004138602.18.2e-7091.67dnaJ homolog subfamily B member 13 [Cucumis sativus] >KGN63200.1 hypothetical pr... [more]
XP_038902656.14.1e-6992.31dnaJ protein homolog 1-like [Benincasa hispida][more]
KAG7010760.19.0e-6990.28DnaJ-like subfamily B member 13 [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_023512074.19.0e-6990.28dnaJ homolog subfamily B member 13-like [Cucurbita pepo subsp. pepo] >KAG6570917... [more]
Match NameE-valueIdentityDescription
P599109.5e-3750.00DnaJ homolog subfamily B member 13 OS=Homo sapiens OX=9606 GN=DNAJB13 PE=1 SV=1[more]
Q80Y751.8e-3547.10DnaJ homolog subfamily B member 13 OS=Mus musculus OX=10090 GN=Dnajb13 PE=1 SV=1[more]
P256857.5e-3446.53DnaJ homolog subfamily B member 1 OS=Homo sapiens OX=9606 GN=DNAJB1 PE=1 SV=4[more]
Q9QYJ37.5e-3446.53DnaJ homolog subfamily B member 1 OS=Mus musculus OX=10090 GN=Dnajb1 PE=1 SV=3[more]
Q3MI009.8e-3446.53DnaJ homolog subfamily B member 1 OS=Bos taurus OX=9913 GN=DNAJB1 PE=2 SV=3[more]
Match NameE-valueIdentityDescription
A0A6J1CFM62.6e-7799.31dnaJ homolog subfamily B member 13-like OS=Momordica charantia OX=3673 GN=LOC111... [more]
A0A0A0LMP64.0e-7091.67J domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G408950 PE=4 SV=1[more]
A0A5D3DA894.4e-6990.28DnaJ-like protein subfamily B member 13-like OS=Cucumis melo var. makuwa OX=1194... [more]
A0A6J1FSM34.4e-6990.28dnaJ homolog subfamily B member 13-like OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
A0A1S3C2294.4e-6990.28dnaJ homolog subfamily B member 13-like OS=Cucumis melo OX=3656 GN=LOC103496013 ... [more]
Match NameE-valueIdentityDescription
AT2G20560.14.2e-6478.47DNAJ heat shock family protein [more]
AT4G28480.11.6e-6075.00DNAJ heat shock family protein [more]
AT4G28480.21.8e-5976.26DNAJ heat shock family protein [more]
AT2G20550.12.9e-5770.14HSP40/DnaJ peptide-binding protein [more]
AT2G20550.22.9e-5770.14HSP40/DnaJ peptide-binding protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D2.60.260.20coord: 49..129
e-value: 3.2E-27
score: 96.7
NoneNo IPR availableGENE3D2.60.260.20coord: 1..47
e-value: 7.0E-18
score: 66.5
NoneNo IPR availablePANTHERPTHR24078:SF529HEAT-SHOCK PROTEIN DNAJcoord: 1..144
NoneNo IPR availablePANTHERPTHR24078DNAJ HOMOLOG SUBFAMILY C MEMBERcoord: 1..144
NoneNo IPR availableCDDcd10747DnaJ_Ccoord: 2..131
e-value: 1.03119E-40
score: 131.779
IPR002939Chaperone DnaJ, C-terminalPFAMPF01556DnaJ_Ccoord: 4..128
e-value: 1.8E-36
score: 125.6
IPR008971HSP40/DnaJ peptide-bindingSUPERFAMILY49493HSP40/DnaJ peptide-binding domaincoord: 7..59
IPR008971HSP40/DnaJ peptide-bindingSUPERFAMILY49493HSP40/DnaJ peptide-binding domaincoord: 52..136

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS004925.1MS004925.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006457 protein folding
molecular_function GO:0051082 unfolded protein binding