MS004769 (gene) Bitter gourd (TR) v1

Overview


Name	MS004769
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	AAI domain-containing protein
Location	scaffold176: 152110 .. 152418 (+)
RNA-Seq Expression	MS004769
Synteny	MS004769

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGATGACCCGGACGGTTGCGGCGTTAGCGGTGGCGGTAGCGGTACTGGTGAGCTTAGCGGGGATATCGGGAGCGGTGAATGTGTGTGGTGTCGACGAGGACGGTCTCACAGCGTGCAAGCCATGGGTGACGAAGCCGTGCCCTACCGATCCCCCGCCCGTCGCTTGCTGCAACGGCCTCTCGAAGGCTGATTTCGGGTGCTTTTGCAACTATAAGAACTCTCTTGTGCTGCCCTCGCTTGGGATTGAACCTGATCTTGCTCTTGCTTTGCCGGCCAAGTGCGGCCTCCCCAATGCTCCCCCATGC

mRNA sequence

ATGGAGATGACCCGGACGGTTGCGGCGTTAGCGGTGGCGGTAGCGGTACTGGTGAGCTTAGCGGGGATATCGGGAGCGGTGAATGTGTGTGGTGTCGACGAGGACGGTCTCACAGCGTGCAAGCCATGGGTGACGAAGCCGTGCCCTACCGATCCCCCGCCCGTCGCTTGCTGCAACGGCCTCTCGAAGGCTGATTTCGGGTGCTTTTGCAACTATAAGAACTCTCTTGTGCTGCCCTCGCTTGGGATTGAACCTGATCTTGCTCTTGCTTTGCCGGCCAAGTGCGGCCTCCCCAATGCTCCCCCATGC

Coding sequence (CDS)

ATGGAGATGACCCGGACGGTTGCGGCGTTAGCGGTGGCGGTAGCGGTACTGGTGAGCTTAGCGGGGATATCGGGAGCGGTGAATGTGTGTGGTGTCGACGAGGACGGTCTCACAGCGTGCAAGCCATGGGTGACGAAGCCGTGCCCTACCGATCCCCCGCCCGTCGCTTGCTGCAACGGCCTCTCGAAGGCTGATTTCGGGTGCTTTTGCAACTATAAGAACTCTCTTGTGCTGCCCTCGCTTGGGATTGAACCTGATCTTGCTCTTGCTTTGCCGGCCAAGTGCGGCCTCCCCAATGCTCCCCCATGC

Protein sequence

MEMTRTVAALAVAVAVLVSLAGISGAVNVCGVDEDGLTACKPWVTKPCPTDPPPVACCNGLSKADFGCFCNYKNSLVLPSLGIEPDLALALPAKCGLPNAPPC

Homology

[Close]

BLAST of MS004769 vs. NCBI nr
Match: XP_022156089.1 (putative lipid-transfer protein DIR1 [Momordica charantia])

HSP 1 Score: 208.8 bits (530), Expect = 2.3e-50
Identity = 101/103 (98.06%), Postives = 103/103 (100.00%), Query Frame = 0


Query: 1   MEMTRTVAALAVAVAVLVSLAGISGAVNVCGVDEDGLTACKPWVTKPCPTDPPPVACCNG 60
           MEMTRTVAALAVAVAVLVSLAGISGAVNVCGVDEDGLTACKPWVTKPCPTDPPPVACCNG
Sbjct: 1   MEMTRTVAALAVAVAVLVSLAGISGAVNVCGVDEDGLTACKPWVTKPCPTDPPPVACCNG 60

Query: 61  LSKADFGCFCNYKNSLVLPSLGIEPDLALALPAKCGLPNAPPC 104
           LSKADFGCFCNYKNSLVLPSLGIEP+LALALPAKCGLP+APPC
Sbjct: 61  LSKADFGCFCNYKNSLVLPSLGIEPNLALALPAKCGLPSAPPC 103

[Close]

BLAST of MS004769 vs. NCBI nr
Match: KAG7025457.1 (putative lipid-transfer protein DIR1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 157.5 bits (397), Expect = 6.1e-35
Identity = 72/102 (70.59%), Postives = 86/102 (84.31%), Query Frame = 0


Query: 2   EMTRTVAALAVAVAVLVSLAGISGAVNVCGVDEDGLTACKPWVTKPCPTDPPPVACCNGL 61
           + T    A+A+ + +LVSL+G    ++VCGV +DGLTACKPWVTKPCPT+ PP ACC+GL
Sbjct: 3   QSTAMTMAMAMTMVMLVSLSGALAEMDVCGVSQDGLTACKPWVTKPCPTELPPAACCDGL 62

Query: 62  SKADFGCFCNYKNSLVLPSLGIEPDLALALPAKCGLPNAPPC 104
           SKADFGCFCNYK+SLVLPSLGI+PDLALALPAKC LPN+P C
Sbjct: 63  SKADFGCFCNYKHSLVLPSLGIDPDLALALPAKCSLPNSPSC 104

[Close]

BLAST of MS004769 vs. NCBI nr
Match: KAG6593049.1 (putative lipid-transfer protein DIR1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 157.1 bits (396), Expect = 7.9e-35
Identity = 71/95 (74.74%), Postives = 84/95 (88.42%), Query Frame = 0


Query: 9   ALAVAVAVLVSLAGISGAVNVCGVDEDGLTACKPWVTKPCPTDPPPVACCNGLSKADFGC 68
           A+A+ + +LVSL+G    ++VCGV +DGLTACKPWVTKPCPT+ PP ACC+GLSKADFGC
Sbjct: 4   AMAMTMVMLVSLSGALAEMDVCGVSQDGLTACKPWVTKPCPTELPPAACCDGLSKADFGC 63

Query: 69  FCNYKNSLVLPSLGIEPDLALALPAKCGLPNAPPC 104
           FCNYK+SLVLPSLGI+PDLALALPAKC LPN+P C
Sbjct: 64  FCNYKHSLVLPSLGIDPDLALALPAKCSLPNSPSC 98

[Close]

BLAST of MS004769 vs. NCBI nr
Match: KAE8646276.1 (hypothetical protein Csa_016013 [Cucumis sativus])

HSP 1 Score: 144.8 bits (364), Expect = 4.1e-31
Identity = 69/103 (66.99%), Postives = 78/103 (75.73%), Query Frame = 0


Query: 1   MEMTRTVAALAVAVAVLVSLAGISGAVNVCGVDEDGLTACKPWVTKPCPTDPPPVACCNG 60
           MEM + V  +   V +LV LAGIS A+ VCGVDEDGLTACKPWVTKPCP + PP ACCN 
Sbjct: 29  MEMAQKVTVM--VVLILVGLAGISIAMEVCGVDEDGLTACKPWVTKPCPAEMPPAACCNK 88

Query: 61  LSKADFGCFCNYKNSLVLPSLGIEPDLALALPAKCGLPNAPPC 104
           LS ADF CFC YKNS++L S GI+ DLALALP KC +PN P C
Sbjct: 89  LSNADFDCFCKYKNSMLLSSFGIDSDLALALPVKCNIPNTPTC 129

[Close]

BLAST of MS004769 vs. NCBI nr
Match: KAA0041998.1 (putative lipid-transfer protein DIR1 [Cucumis melo var. makuwa] >TYK17933.1 putative lipid-transfer protein DIR1 [Cucumis melo var. makuwa])

HSP 1 Score: 142.1 bits (357), Expect = 2.6e-30
Identity = 67/103 (65.05%), Postives = 78/103 (75.73%), Query Frame = 0


Query: 1   MEMTRTVAALAVAVAVLVSLAGISGAVNVCGVDEDGLTACKPWVTKPCPTDPPPVACCNG 60
           MEM   V  +  AV +LV +AGIS A+ +CGVDE+GLTACKPWVTKPCPT+ PP  CCN 
Sbjct: 1   MEMALKVKIM--AVVILVGIAGISIAMEMCGVDEEGLTACKPWVTKPCPTEMPPATCCNK 60

Query: 61  LSKADFGCFCNYKNSLVLPSLGIEPDLALALPAKCGLPNAPPC 104
           LS ADF CFC YKNS++L S GI+PDLALALP KC + N P C
Sbjct: 61  LSNADFSCFCKYKNSMLLSSFGIDPDLALALPVKCNITNTPTC 101

[Close]

BLAST of MS004769 vs. ExPASy Swiss-Prot
Match: Q8W453 (Putative lipid-transfer protein DIR1 OS=Arabidopsis thaliana OX=3702 GN=DIR1 PE=1 SV=1)

HSP 1 Score: 88.2 bits (217), Expect = 5.9e-17
Identity = 44/103 (42.72%), Postives = 59/103 (57.28%), Query Frame = 0


Query: 1   MEMTRTVAALAVAVAVLVSLAGISGAVNVCGVDEDGLTACKPWVTKPCPTDPPPVACCNG 60
           M   +    +   + ++  L   S A+++CG+ +D L  CKP V+K  PT P    CC  
Sbjct: 1   MASKKAAMVMMAMIVIMAMLVDTSVAIDLCGMSQDELNECKPAVSKENPTSPSQ-PCCTA 60

Query: 61  LSKADFGCFCNYKNSLVLPSLGIEPDLALALPAKCGLPNAPPC 104
           L  ADF C C YKNS  L S G++P+LA ALP +CGL NAP C
Sbjct: 61  LQHADFACLCGYKNSPWLGSFGVDPELASALPKQCGLANAPTC 102

[Close]

BLAST of MS004769 vs. ExPASy TrEMBL
Match: A0A6J1DS99 (putative lipid-transfer protein DIR1 OS=Momordica charantia OX=3673 GN=LOC111023061 PE=4 SV=1)

HSP 1 Score: 208.8 bits (530), Expect = 1.1e-50
Identity = 101/103 (98.06%), Postives = 103/103 (100.00%), Query Frame = 0


Query: 1   MEMTRTVAALAVAVAVLVSLAGISGAVNVCGVDEDGLTACKPWVTKPCPTDPPPVACCNG 60
           MEMTRTVAALAVAVAVLVSLAGISGAVNVCGVDEDGLTACKPWVTKPCPTDPPPVACCNG
Sbjct: 1   MEMTRTVAALAVAVAVLVSLAGISGAVNVCGVDEDGLTACKPWVTKPCPTDPPPVACCNG 60

Query: 61  LSKADFGCFCNYKNSLVLPSLGIEPDLALALPAKCGLPNAPPC 104
           LSKADFGCFCNYKNSLVLPSLGIEP+LALALPAKCGLP+APPC
Sbjct: 61  LSKADFGCFCNYKNSLVLPSLGIEPNLALALPAKCGLPSAPPC 103

[Close]

BLAST of MS004769 vs. ExPASy TrEMBL
Match: A0A0A0K4Q7 (AAI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G372360 PE=4 SV=1)

HSP 1 Score: 144.8 bits (364), Expect = 2.0e-31
Identity = 69/103 (66.99%), Postives = 78/103 (75.73%), Query Frame = 0


Query: 1   MEMTRTVAALAVAVAVLVSLAGISGAVNVCGVDEDGLTACKPWVTKPCPTDPPPVACCNG 60
           MEM + V  +   V +LV LAGIS A+ VCGVDEDGLTACKPWVTKPCP + PP ACCN 
Sbjct: 1   MEMAQKVTVM--VVLILVGLAGISIAMEVCGVDEDGLTACKPWVTKPCPAEMPPAACCNK 60

Query: 61  LSKADFGCFCNYKNSLVLPSLGIEPDLALALPAKCGLPNAPPC 104
           LS ADF CFC YKNS++L S GI+ DLALALP KC +PN P C
Sbjct: 61  LSNADFDCFCKYKNSMLLSSFGIDSDLALALPVKCNIPNTPTC 101

[Close]

BLAST of MS004769 vs. ExPASy TrEMBL
Match: A0A5D3D158 (Putative lipid-transfer protein DIR1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold306G002300 PE=4 SV=1)

HSP 1 Score: 142.1 bits (357), Expect = 1.3e-30
Identity = 67/103 (65.05%), Postives = 78/103 (75.73%), Query Frame = 0


Query: 1   MEMTRTVAALAVAVAVLVSLAGISGAVNVCGVDEDGLTACKPWVTKPCPTDPPPVACCNG 60
           MEM   V  +  AV +LV +AGIS A+ +CGVDE+GLTACKPWVTKPCPT+ PP  CCN 
Sbjct: 1   MEMALKVKIM--AVVILVGIAGISIAMEMCGVDEEGLTACKPWVTKPCPTEMPPATCCNK 60

Query: 61  LSKADFGCFCNYKNSLVLPSLGIEPDLALALPAKCGLPNAPPC 104
           LS ADF CFC YKNS++L S GI+PDLALALP KC + N P C
Sbjct: 61  LSNADFSCFCKYKNSMLLSSFGIDPDLALALPVKCNITNTPTC 101

[Close]

BLAST of MS004769 vs. ExPASy TrEMBL
Match: A0A1S3BHS9 (putative lipid-transfer protein DIR1 OS=Cucumis melo OX=3656 GN=LOC103489717 PE=4 SV=1)

HSP 1 Score: 141.0 bits (354), Expect = 2.8e-30
Identity = 67/103 (65.05%), Postives = 77/103 (74.76%), Query Frame = 0


Query: 1   MEMTRTVAALAVAVAVLVSLAGISGAVNVCGVDEDGLTACKPWVTKPCPTDPPPVACCNG 60
           MEM   V     AV +LV +AGIS A+ +CGVDE+GLTACKPWVTKPCPT+ PP  CCN 
Sbjct: 1   MEMALKVK--ITAVVILVGIAGISIAMEMCGVDEEGLTACKPWVTKPCPTEMPPATCCNK 60

Query: 61  LSKADFGCFCNYKNSLVLPSLGIEPDLALALPAKCGLPNAPPC 104
           LS ADF CFC YKNS++L S GI+PDLALALP KC + N P C
Sbjct: 61  LSNADFSCFCKYKNSMLLSSFGIDPDLALALPVKCNITNTPTC 101

[Close]

BLAST of MS004769 vs. ExPASy TrEMBL
Match: A0A6J1GPI5 (putative lipid-transfer protein DIR1 OS=Cucurbita moschata OX=3662 GN=LOC111456291 PE=4 SV=1)

HSP 1 Score: 135.2 bits (339), Expect = 1.6e-28
Identity = 63/96 (65.62%), Postives = 77/96 (80.21%), Query Frame = 0


Query: 8   AALAVAVAVLVSLAGISGAVNVCGVDEDGLTACKPWVTKPCPTDPPPVACCNGLSKADFG 67
           A   V + ++VSLA ++ AV+VCGV++DGL ACKPWV KPCPT+ PP ACC+GLSKAD G
Sbjct: 5   APKVVVMVMVVSLAEMAMAVDVCGVNKDGLKACKPWVMKPCPTELPPTACCDGLSKADLG 64

Query: 68  CFCNYKNSLVLPSLGIEPDLALALPAKCGLPNAPPC 104
           CFC YK+S++L SLGI+ DLAL LPAKC LPN P C
Sbjct: 65  CFCKYKHSMLLSSLGIDLDLALGLPAKCSLPNTPSC 100

[Close]

BLAST of MS004769 vs. TAIR 10
Match: AT5G48485.1 (Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein )

HSP 1 Score: 88.2 bits (217), Expect = 4.2e-18
Identity = 44/103 (42.72%), Postives = 59/103 (57.28%), Query Frame = 0


Query: 1   MEMTRTVAALAVAVAVLVSLAGISGAVNVCGVDEDGLTACKPWVTKPCPTDPPPVACCNG 60
           M   +    +   + ++  L   S A+++CG+ +D L  CKP V+K  PT P    CC  
Sbjct: 1   MASKKAAMVMMAMIVIMAMLVDTSVAIDLCGMSQDELNECKPAVSKENPTSPSQ-PCCTA 60

Query: 61  LSKADFGCFCNYKNSLVLPSLGIEPDLALALPAKCGLPNAPPC 104
           L  ADF C C YKNS  L S G++P+LA ALP +CGL NAP C
Sbjct: 61  LQHADFACLCGYKNSPWLGSFGVDPELASALPKQCGLANAPTC 102

[Close]

BLAST of MS004769 vs. TAIR 10
Match: AT5G48490.1 (Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein )

HSP 1 Score: 79.0 bits (193), Expect = 2.6e-15
Identity = 46/102 (45.10%), Postives = 60/102 (58.82%), Query Frame = 0


Query: 3   MTRTVAALAVAVAVLVSL-AGISGAVNVCGVDEDGLTACKPWVTKPCPTDPPPVACCNGL 62
           MT    A+ V V V+ SL    S A+++CG+ +  L  C P V+K  PT  P + CCN L
Sbjct: 1   MTSKKVAIMVIVMVMASLVVERSVAIDLCGMTQAELNECLPAVSKNNPTS-PSLLCCNAL 60

Query: 63  SKADFGCFCNYKNSLVLPSLGIEPDLALALPAKCGLPNAPPC 104
             AD+ C C YKNS  L S G++P LA +LP +C L NAP C
Sbjct: 61  KHADYTCLCGYKNSPWLGSFGVDPKLASSLPKECDLTNAPTC 101

The following BLAST results are available for this feature:

BLAST of MS004769 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022156089.1	2.3e-50	98.06	putative lipid-transfer protein DIR1 [Momordica charantia]	[more]
KAG7025457.1	6.1e-35	70.59	putative lipid-transfer protein DIR1, partial [Cucurbita argyrosperma subsp. arg...	[more]
KAG6593049.1	7.9e-35	74.74	putative lipid-transfer protein DIR1, partial [Cucurbita argyrosperma subsp. sor...	[more]
KAE8646276.1	4.1e-31	66.99	hypothetical protein Csa_016013 [Cucumis sativus]	[more]
KAA0041998.1	2.6e-30	65.05	putative lipid-transfer protein DIR1 [Cucumis melo var. makuwa] >TYK17933.1 puta...	[more]

BLAST of MS004769 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8W453	5.9e-17	42.72	Putative lipid-transfer protein DIR1 OS=Arabidopsis thaliana OX=3702 GN=DIR1 PE=...	[more]

BLAST of MS004769 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DS99	1.1e-50	98.06	putative lipid-transfer protein DIR1 OS=Momordica charantia OX=3673 GN=LOC111023...	[more]
A0A0A0K4Q7	2.0e-31	66.99	AAI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G372360 PE=4 SV...	[more]
A0A5D3D158	1.3e-30	65.05	Putative lipid-transfer protein DIR1 OS=Cucumis melo var. makuwa OX=1194695 GN=E...	[more]
A0A1S3BHS9	2.8e-30	65.05	putative lipid-transfer protein DIR1 OS=Cucumis melo OX=3656 GN=LOC103489717 PE=...	[more]
A0A6J1GPI5	1.6e-28	65.63	putative lipid-transfer protein DIR1 OS=Cucurbita moschata OX=3662 GN=LOC1114562...	[more]

BLAST of MS004769 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G48485.1	4.2e-18	42.72	Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamil...	[more]
AT5G48490.1	2.6e-15	45.10	Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamil...	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR016140	Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain	SMART	SM00499	aai_6	coord: 40..103 e-value: 4.6E-4 score: 29.5
IPR016140	Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain	PFAM	PF14368	LTP_2	coord: 14..101 e-value: 1.8E-9 score: 37.6
IPR036312	Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain superfamily	GENE3D	1.10.110.10		coord: 26..103 e-value: 2.8E-19 score: 70.7
IPR036312	Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain superfamily	SUPERFAMILY	47699	Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin	coord: 30..102
IPR039265	Putative lipid-transfer protein DIR1-like	PANTHER	PTHR33122	LIPID BINDING PROTEIN-RELATED	coord: 7..103
None	No IPR available	PANTHER	PTHR33122:SF63	BIFUNCTIONAL INHIBITOR/LIPID-TRANSFER PROTEIN/SEED STORAGE 2S ALBUMIN SUPERFAMILY PROTEIN	coord: 7..103
IPR044741	Non-specific lipid-transfer protein-like	CDD	cd04660	nsLTP_like	coord: 30..102 e-value: 8.64971E-18 score: 69.3202

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS004769.1	MS004769.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009627	systemic acquired resistance
molecular_function	GO:0005504	fatty acid binding