MS004338 (gene) Bitter gourd (TR) v1

Overview


Name	MS004338
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Eukaryotic aspartyl protease family protein
Location	scaffold92: 1956734 .. 1958906 (-)
RNA-Seq Expression	MS004338
Synteny	MS004338

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

AAAATACCCTTCCCCCTCCTCTTCCTTCTCCATCTCCTCATCTTCTCCGCCGCCGCCGCCGCCGACTTCCAATTTCTTAACGTCAAAGAAACCAAATTCGACTCCAACGACGATCGGATTCTCCACAACCTTAACTTCTCCGACGGCCACCGTCAAGTTACCGGTAAAAAATCCAACAGTAGCAGTAGCACTAAATTCAAGCTGAAGCTCGTCCACCGGGATAAGCTCCCGCCGTCCCACGTCCACGGCCGCCGCCACGGCTTCGTTGAGCGCATGAAAAGAGACGCCTCAAGGGTCGCCGCCCTCGTCCGCCGCCTGTCCCACGGCGGCGCCGACAGCAGTTACAAGGCGGCGAATTTCGCGACGGATGTCATTTCAGGCAAGGGAAATTATGAATAAAAAAATTAAAACTTTTTAAATTTTTTTAATACCCTTTTGGGATTTATTTATTTATTTGTTTTCGTTGGTGATTTGGAATTAGGGATGGAAGAAGGTAGTGGAGAATATTTTGTTCGGATCGGGGTTGGTAGCCCGCCGAGGAGTCAGTATATGGTTATTGATTCCGGTAGTGATATTGTGTGGGTCCAATGTCAGCCTTGTAGCCAATGCTACCAACAGTCCGACCCGGTTTTCGACCCGGCCGATTCCGCCTCTTTCACCGGCGTCTCCTGTGGCTCCGCTCTTTGCGACCGCCTTGAGAATGACGCTGGCTGCCACGCTGGACGGTGCCGATATGAGGTGTTGTATGGCGATGGGTCTTACACCAAGGGTACTCTCGCTCTCGAAACTCTCACCATCGGCGAGGTGAGTTGAATTCCTACCTAACTCAAAAGTTTAAATAAGTAGGTGAAAATATATTTACTTAAAGCTAACTTGCATGTAATTTTATGATTGTGTATAAATTATTCGGTCCATATATATATTTTAAATAATATTAACTAATAATGATACTGTCATTCAAAAATTGACAGTTTATTAATTTTTTTCTTTTAAATTATCTATGTTTGCACTTCTTTTTATTAGTTGACAAATATTTAACAGTAGTGAGCAGTCCTCTTACTTGACATAAGAATTTATAATTTTTTTTTTCAATACAATAAATTGTGAGTATGAGAGATCGAATCTCCAAAGTCGAGAGAATAAATAGATTCTAACTATCATTGAGATATGTTCATGTTATCATAATTCATAATGAGTAGGTTATTGAATATAGGTTACAAGGATTTCAAGATTTTTTCTTTTTCCAAATTAAAGTTTAAATTTTTTTTTATAAGAATTATGAGTCCTAGTTTTGAAAATTTAAGATTTATTACAAAACTTTTTAAAAATAAATACAAAGACGAAACTTATAAATTTAAACCTTTTGTTAAACAGTGAAACTGGAAGAAAATGAGATTACAATTATTCTAAATATATTTAATCATTTATTTATTTATATATTTTTAAAAAGGTCGTGGTTCGGGATGTGGCGATCGGATGCGGTCATACGAACCAGGGCATGTTCATCGGAGCCGCCGGTTTACTTGGTCTCGGCGGCGGCTCCATGTCGTTCGTCGGCCAGCTCGGCGGTCAGACTGGCGGAGCATTCAGCTACTGTTTGGTGAGTAGAGGGTCCGGTTCGACCGGAGCATTAGAATTCGGCCGGGGAGCACTGCCGGTCGGCGCCACGTGGATCTCCCTAATCCGAAACCCACGCGCCCCAAGCTTCTACTACATCGGACTCGCCGGCGTCGGCGTCGGCGGCGTCAGAGTACCGGTGCCGGAAGAAACTTACCGGCTAACCGAGTACGGCAGCAACGGCGTCGTAATGGACACCGGCACCGCCGTGACGCGGCTGCCGACGGCGGCGTACGAGGCGCTCCGAGACTCCTTCACGGCGCAGACCGCGAACCTACCACGAGCGGGCGCGGTTTCGATCTTCGACACGTGTTACGATCTGAACGGGTTCGAGTCCGTACGGGTGCCAACGGTGTCGTTTTACTTTTCGGACGGGCCGGTGCTGACGCTTCCGGCGAAGAACTTCCTGATTCCGGTGAACGGCGCCGGAACTTTTTGCTTTGCGTTTGCTCCGTCGCCGTCGGGGCTTTCCATAATCGGAAACATCCAGCAGGAGGGAATTCAAATTTCCTTCGATGGGGCTAATGGATTTGTGGGATTTGGCCCAAATGTTTGC

mRNA sequence

AAAATACCCTTCCCCCTCCTCTTCCTTCTCCATCTCCTCATCTTCTCCGCCGCCGCCGCCGCCGACTTCCAATTTCTTAACGTCAAAGAAACCAAATTCGACTCCAACGACGATCGGATTCTCCACAACCTTAACTTCTCCGACGGCCACCGTCAAGTTACCGGTAAAAAATCCAACAGTAGCAGTAGCACTAAATTCAAGCTGAAGCTCGTCCACCGGGATAAGCTCCCGCCGTCCCACGTCCACGGCCGCCGCCACGGCTTCGTTGAGCGCATGAAAAGAGACGCCTCAAGGGTCGCCGCCCTCGTCCGCCGCCTGTCCCACGGCGGCGCCGACAGCAGGATGGAAGAAGGTAGTGGAGAATATTTTGTTCGGATCGGGGTTGGTAGCCCGCCGAGGAGTCAGTATATGGTTATTGATTCCGGTAGTGATATTGTGTGGGTCCAATGTCAGCCTTGTAGCCAATGCTACCAACAGTCCGACCCGGTTTTCGACCCGGCCGATTCCGCCTCTTTCACCGGCGTCTCCTGTGGCTCCGCTCTTTGCGACCGCCTTGAGAATGACGCTGGCTGCCACGCTGGACGGTGCCGATATGAGGTGTTGTATGGCGATGGGTCTTACACCAAGGGTACTCTCGCTCTCGAAACTCTCACCATCGGCGAGGTCGTGGTTCGGGATGTGGCGATCGGATGCGGTCATACGAACCAGGGCATGTTCATCGGAGCCGCCGGTTTACTTGGTCTCGGCGGCGGCTCCATGTCGTTCGTCGGCCAGCTCGGCGGTCAGACTGGCGGAGCATTCAGCTACTGTTTGGTGAGTAGAGGGTCCGGTTCGACCGGAGCATTAGAATTCGGCCGGGGAGCACTGCCGGTCGGCGCCACGTGGATCTCCCTAATCCGAAACCCACGCGCCCCAAGCTTCTACTACATCGGACTCGCCGGCGTCGGCGTCGGCGGCGTCAGAGTACCGGTGCCGGAAGAAACTTACCGGCTAACCGAGTACGGCAGCAACGGCGTCGTAATGGACACCGGCACCGCCGTGACGCGGCTGCCGACGGCGGCGTACGAGGCGCTCCGAGACTCCTTCACGGCGCAGACCGCGAACCTACCACGAGCGGGCGCGGTTTCGATCTTCGACACGTGTTACGATCTGAACGGGTTCGAGTCCGTACGGGTGCCAACGGTGTCGTTTTACTTTTCGGACGGGCCGGTGCTGACGCTTCCGGCGAAGAACTTCCTGATTCCGGTGAACGGCGCCGGAACTTTTTGCTTTGCGTTTGCTCCGTCGCCGTCGGGGCTTTCCATAATCGGAAACATCCAGCAGGAGGGAATTCAAATTTCCTTCGATGGGGCTAATGGATTTGTGGGATTTGGCCCAAATGTTTGC

Coding sequence (CDS)

AAAATACCCTTCCCCCTCCTCTTCCTTCTCCATCTCCTCATCTTCTCCGCCGCCGCCGCCGCCGACTTCCAATTTCTTAACGTCAAAGAAACCAAATTCGACTCCAACGACGATCGGATTCTCCACAACCTTAACTTCTCCGACGGCCACCGTCAAGTTACCGGTAAAAAATCCAACAGTAGCAGTAGCACTAAATTCAAGCTGAAGCTCGTCCACCGGGATAAGCTCCCGCCGTCCCACGTCCACGGCCGCCGCCACGGCTTCGTTGAGCGCATGAAAAGAGACGCCTCAAGGGTCGCCGCCCTCGTCCGCCGCCTGTCCCACGGCGGCGCCGACAGCAGGATGGAAGAAGGTAGTGGAGAATATTTTGTTCGGATCGGGGTTGGTAGCCCGCCGAGGAGTCAGTATATGGTTATTGATTCCGGTAGTGATATTGTGTGGGTCCAATGTCAGCCTTGTAGCCAATGCTACCAACAGTCCGACCCGGTTTTCGACCCGGCCGATTCCGCCTCTTTCACCGGCGTCTCCTGTGGCTCCGCTCTTTGCGACCGCCTTGAGAATGACGCTGGCTGCCACGCTGGACGGTGCCGATATGAGGTGTTGTATGGCGATGGGTCTTACACCAAGGGTACTCTCGCTCTCGAAACTCTCACCATCGGCGAGGTCGTGGTTCGGGATGTGGCGATCGGATGCGGTCATACGAACCAGGGCATGTTCATCGGAGCCGCCGGTTTACTTGGTCTCGGCGGCGGCTCCATGTCGTTCGTCGGCCAGCTCGGCGGTCAGACTGGCGGAGCATTCAGCTACTGTTTGGTGAGTAGAGGGTCCGGTTCGACCGGAGCATTAGAATTCGGCCGGGGAGCACTGCCGGTCGGCGCCACGTGGATCTCCCTAATCCGAAACCCACGCGCCCCAAGCTTCTACTACATCGGACTCGCCGGCGTCGGCGTCGGCGGCGTCAGAGTACCGGTGCCGGAAGAAACTTACCGGCTAACCGAGTACGGCAGCAACGGCGTCGTAATGGACACCGGCACCGCCGTGACGCGGCTGCCGACGGCGGCGTACGAGGCGCTCCGAGACTCCTTCACGGCGCAGACCGCGAACCTACCACGAGCGGGCGCGGTTTCGATCTTCGACACGTGTTACGATCTGAACGGGTTCGAGTCCGTACGGGTGCCAACGGTGTCGTTTTACTTTTCGGACGGGCCGGTGCTGACGCTTCCGGCGAAGAACTTCCTGATTCCGGTGAACGGCGCCGGAACTTTTTGCTTTGCGTTTGCTCCGTCGCCGTCGGGGCTTTCCATAATCGGAAACATCCAGCAGGAGGGAATTCAAATTTCCTTCGATGGGGCTAATGGATTTGTGGGATTTGGCCCAAATGTTTGC

Protein sequence

KIPFPLLFLLHLLIFSAAAAADFQFLNVKETKFDSNDDRILHNLNFSDGHRQVTGKKSNSSSSTKFKLKLVHRDKLPPSHVHGRRHGFVERMKRDASRVAALVRRLSHGGADSRMEEGSGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQCQPCSQCYQQSDPVFDPADSASFTGVSCGSALCDRLENDAGCHAGRCRYEVLYGDGSYTKGTLALETLTIGEVVVRDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFVGQLGGQTGGAFSYCLVSRGSGSTGALEFGRGALPVGATWISLIRNPRAPSFYYIGLAGVGVGGVRVPVPEETYRLTEYGSNGVVMDTGTAVTRLPTAAYEALRDSFTAQTANLPRAGAVSIFDTCYDLNGFESVRVPTVSFYFSDGPVLTLPAKNFLIPVNGAGTFCFAFAPSPSGLSIIGNIQQEGIQISFDGANGFVGFGPNVC

Homology

[Close]

BLAST of MS004338 vs. NCBI nr
Match: XP_038893071.1 (protein ASPARTIC PROTEASE IN GUARD CELL 2-like [Benincasa hispida])

HSP 1 Score: 766.1 bits (1977), Expect = 1.7e-217
Identity = 395/482 (81.95%), Postives = 420/482 (87.14%), Query Frame = 0


Query: 1   KIPFPLLFLLHLLIFSAAAA----ADFQFLNVKETKFDSNDDRILHNLNFSDGHRQVTGK 60
           K P  LLF L  L FSAAA       FQ+LNVK TK D NDD+ILH LNFS   RQV+G 
Sbjct: 4   KQPSSLLFFLFFLFFSAAAGRSSPTKFQYLNVKATKLDFNDDQILHTLNFSHARRQVSGH 63

Query: 61  KSNSSSSTKFKLKLVHRDKLPPSHVHGRRHGFVERMKRDASRVAALVRRLSHGG------ 120
           KS++++   FKL L+HRDKL  SHVHG RHGF ER+KRDA RVA LVRRLSHGG      
Sbjct: 64  KSDNNT---FKLNLLHRDKL--SHVHGHRHGFNERIKRDAIRVATLVRRLSHGGGAVQDN 123

Query: 121 ----AD------SRMEEGSGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQCQPCSQCYQQS 180
               AD      S MEEGSGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQC+PCS+CYQQS
Sbjct: 124 KYKVADFATDVISGMEEGSGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQCKPCSRCYQQS 183

Query: 181 DPVFDPADSASFTGVSCGSALCDRLENDAGCHAGRCRYEVLYGDGSYTKGTLALETLTIG 240
           DPVFDPADS+SF GVSC S +CDRLEN  GC+AGRCRYEV YGDGSYTKGTLALETLT+G
Sbjct: 184 DPVFDPADSSSFAGVSCDSIVCDRLEN-TGCNAGRCRYEVSYGDGSYTKGTLALETLTVG 243

Query: 241 EVVVRDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFVGQLGGQTGGAFSYCLVSRGSGSTG 300
           EV++RDVAIGCGHTNQGMFIGAAGLLGLGGGSMSF+GQLGGQTGGAFSYCLVSRG+GSTG
Sbjct: 244 EVMIRDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFIGQLGGQTGGAFSYCLVSRGTGSTG 303

Query: 301 ALEFGRGALPVGATWISLIRNPRAPSFYYIGLAGVGVGGVRVPVPEETYRLTEYGSNGVV 360
            LEFGRGALPVGATWISLIRNPRAPSFYYIGLAG+GVGGVRV VPEET++LTEYG+NGVV
Sbjct: 304 TLEFGRGALPVGATWISLIRNPRAPSFYYIGLAGIGVGGVRVSVPEETFQLTEYGTNGVV 363

Query: 361 MDTGTAVTRLPTAAYEALRDSFTAQTANLPRAGAVSIFDTCYDLNGFESVRVPTVSFYFS 420
           MDTGTAVTRLPTAAY ALRDSFTAQT+NLPRA  VSIFDTCYDLNGFESVRVPTVSFYFS
Sbjct: 364 MDTGTAVTRLPTAAYVALRDSFTAQTSNLPRAPGVSIFDTCYDLNGFESVRVPTVSFYFS 423

Query: 421 DGPVLTLPAKNFLIPVNGAGTFCFAFAPSPSGLSIIGNIQQEGIQISFDGANGFVGFGPN 463
           DGPVLTLPAKNFLIPV+G GTFC AFAPSPSGLSIIGNIQQEGIQISFDGANGFVGFGPN
Sbjct: 424 DGPVLTLPAKNFLIPVDGGGTFCLAFAPSPSGLSIIGNIQQEGIQISFDGANGFVGFGPN 479

[Close]

BLAST of MS004338 vs. NCBI nr
Match: XP_008445900.1 (PREDICTED: protein ASPARTIC PROTEASE IN GUARD CELL 2-like [Cucumis melo])

HSP 1 Score: 757.7 bits (1955), Expect = 5.9e-215
Identity = 387/478 (80.96%), Postives = 418/478 (87.45%), Query Frame = 0


Query: 6   LLFLLHLLIFSAAAA-----ADFQFLNVKETKFDSNDDRILHNLNFSDGHRQVTGKKSNS 65
           LLF L +L+  AAAA       FQ+LNVK TK D ND +ILH LNFSD HRQV+G KS++
Sbjct: 10  LLFFLAVLVVVAAAARPSSPTKFQYLNVKATKLDFNDGQILHTLNFSDAHRQVSGHKSHN 69

Query: 66  SSSTKFKLKLVHRDKLPPSHVHGRRHGFVERMKRDASRVAALVRRLSHGGA---DSR--- 125
            +   FKL L+HRDKL  SHVHG R GF +RMKRDA RVA LVRRLSHG A   DSR   
Sbjct: 70  DT---FKLNLLHRDKL--SHVHGHRRGFNDRMKRDAIRVATLVRRLSHGAAAVEDSRFKV 129

Query: 126 ----------MEEGSGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQCQPCSQCYQQSDPVF 185
                     MEEGSGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQC+PCS+CYQQSDPVF
Sbjct: 130 ANFATDVISGMEEGSGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQCKPCSRCYQQSDPVF 189

Query: 186 DPADSASFTGVSCGSALCDRLENDAGCHAGRCRYEVLYGDGSYTKGTLALETLTIGEVVV 245
           DPADS+SF GVSCGS +CDRLEN  GC+AGRCRYEV YGDGSYTKGTLALETLT+G+V++
Sbjct: 190 DPADSSSFAGVSCGSDVCDRLEN-TGCNAGRCRYEVSYGDGSYTKGTLALETLTVGQVMI 249

Query: 246 RDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFVGQLGGQTGGAFSYCLVSRGSGSTGALEF 305
           RDVAIGCGHTNQGMFIGAAGLLGLGGGSMSF+GQLGGQTGGAFSYCLVSRG+GSTG LEF
Sbjct: 250 RDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFIGQLGGQTGGAFSYCLVSRGTGSTGTLEF 309

Query: 306 GRGALPVGATWISLIRNPRAPSFYYIGLAGVGVGGVRVPVPEETYRLTEYGSNGVVMDTG 365
           GRGALPVGATWISLIRNPRAPSFYYIGLAG+GVGGVRV +PEET++LTE+G+NGVVMDTG
Sbjct: 310 GRGALPVGATWISLIRNPRAPSFYYIGLAGIGVGGVRVSIPEETFQLTEFGTNGVVMDTG 369

Query: 366 TAVTRLPTAAYEALRDSFTAQTANLPRAGAVSIFDTCYDLNGFESVRVPTVSFYFSDGPV 425
           TAVTRLPT+AY ALRDSFTAQT+NLPRA  VSIFDTCYDLNGFESVRVPTVSFYFSDGP 
Sbjct: 370 TAVTRLPTSAYVALRDSFTAQTSNLPRAPGVSIFDTCYDLNGFESVRVPTVSFYFSDGPA 429

Query: 426 LTLPAKNFLIPVNGAGTFCFAFAPSPSGLSIIGNIQQEGIQISFDGANGFVGFGPNVC 463
           LTLPAKNFLIPV+G GTFC AFAPSPSGLSIIGNIQQEGIQISFDGANGFVGFGPN+C
Sbjct: 430 LTLPAKNFLIPVDGGGTFCLAFAPSPSGLSIIGNIQQEGIQISFDGANGFVGFGPNIC 481

[Close]

BLAST of MS004338 vs. NCBI nr
Match: KAA0034086.1 (protein ASPARTIC PROTEASE IN GUARD CELL 2-like [Cucumis melo var. makuwa])

HSP 1 Score: 756.9 bits (1953), Expect = 1.0e-214
Identity = 384/465 (82.58%), Postives = 416/465 (89.46%), Query Frame = 0


Query: 6   LLFLLHLLIFSAAAA-----ADFQFLNVKETKFDSNDDRILHNLNFSDGHRQVTGKKSNS 65
           LLF L +L+  AAAA       FQ+LNVK TK D ND +ILH LNFSD HRQV+G KS++
Sbjct: 10  LLFFLAVLVVVAAAARPSSPTKFQYLNVKATKLDFNDGQILHTLNFSDAHRQVSGHKSHN 69

Query: 66  SSSTKFKLKLVHRDKLPPSHVHGRRHGFVERMKRDASRVAALVRRLSHGGA---DSRMEE 125
            +   FKL L+HRDKL  SHVHG R GF +RMKRDA RVA LVRRLSHG A   DSR  +
Sbjct: 70  DT---FKLNLLHRDKL--SHVHGHRRGFNDRMKRDAIRVATLVRRLSHGAAAVEDSRF-K 129

Query: 126 GSGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQCQPCSQCYQQSDPVFDPADSASFTGVSC 185
           GSGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQC+PCS+CYQQSDPVFDPADS+SF GVSC
Sbjct: 130 GSGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQCKPCSRCYQQSDPVFDPADSSSFAGVSC 189

Query: 186 GSALCDRLENDAGCHAGRCRYEVLYGDGSYTKGTLALETLTIGEVVVRDVAIGCGHTNQG 245
           GS +CDRLEN  GC+AGRCRYEV YGDGSYTKGTLALETLT+G+V++RDVAIGCGHTNQG
Sbjct: 190 GSDVCDRLEN-TGCNAGRCRYEVSYGDGSYTKGTLALETLTVGQVMIRDVAIGCGHTNQG 249

Query: 246 MFIGAAGLLGLGGGSMSFVGQLGGQTGGAFSYCLVSRGSGSTGALEFGRGALPVGATWIS 305
           MFIGAAGLLGLGGGSMSF+GQLGGQTGGAFSYCLVSRG+GSTG LEFGRGALPVGATWIS
Sbjct: 250 MFIGAAGLLGLGGGSMSFIGQLGGQTGGAFSYCLVSRGTGSTGTLEFGRGALPVGATWIS 309

Query: 306 LIRNPRAPSFYYIGLAGVGVGGVRVPVPEETYRLTEYGSNGVVMDTGTAVTRLPTAAYEA 365
           LIRNPRAPSFYYIGLAG+GVGGVRV +PEET++LTE+G+NGVVMDTGTAVTRLPT+AY A
Sbjct: 310 LIRNPRAPSFYYIGLAGIGVGGVRVSIPEETFQLTEFGTNGVVMDTGTAVTRLPTSAYVA 369

Query: 366 LRDSFTAQTANLPRAGAVSIFDTCYDLNGFESVRVPTVSFYFSDGPVLTLPAKNFLIPVN 425
           LRDSFTAQT+NLPRA  VSIFDTCYDLNGFESVRVPTVSFYFSDGP LTLPAKNFLIPV+
Sbjct: 370 LRDSFTAQTSNLPRAPGVSIFDTCYDLNGFESVRVPTVSFYFSDGPALTLPAKNFLIPVD 429

Query: 426 GAGTFCFAFAPSPSGLSIIGNIQQEGIQISFDGANGFVGFGPNVC 463
           G GTFC AFAPSPSGLSIIGNIQQEGIQISFDGANGFVGFGPN+C
Sbjct: 430 GGGTFCLAFAPSPSGLSIIGNIQQEGIQISFDGANGFVGFGPNIC 467

[Close]

BLAST of MS004338 vs. NCBI nr
Match: XP_004147103.1 (protein ASPARTIC PROTEASE IN GUARD CELL 2 [Cucumis sativus] >KGN51568.1 hypothetical protein Csa_008597 [Cucumis sativus])

HSP 1 Score: 756.1 bits (1951), Expect = 1.7e-214
Identity = 387/479 (80.79%), Postives = 420/479 (87.68%), Query Frame = 0


Query: 6   LLFLLHLLIFSAAAA-----ADFQFLNVKETKFDSNDDRILHNLNFSDGHRQVTGKKSNS 65
           LLFLL +++ +AAAA       FQ+LNVK TK D ND +ILH LNFSDGHRQV+G KS++
Sbjct: 10  LLFLLVVIVGAAAAARLSSSTKFQYLNVKATKLDFNDGQILHALNFSDGHRQVSGYKSDN 69

Query: 66  SSSTKFKLKLVHRDKLPPSHVHGRRHGFVERMKRDASRVAALVRRLSHGG----ADSR-- 125
           ++   FKL L+HRDKL  SHVHG R GF +RMKRDA RVA LVRRLSHG      DSR  
Sbjct: 70  NT---FKLNLLHRDKL--SHVHGHRRGFNDRMKRDAIRVATLVRRLSHGAPAAVKDSRYK 129

Query: 126 -----------MEEGSGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQCQPCSQCYQQSDPV 185
                      ME GSGEYFVRIGVGSPPR+QYMVIDSGSDIVWVQC+PCS+CYQQSDPV
Sbjct: 130 VANFATDVISGMEAGSGEYFVRIGVGSPPRNQYMVIDSGSDIVWVQCKPCSRCYQQSDPV 189

Query: 186 FDPADSASFTGVSCGSALCDRLENDAGCHAGRCRYEVLYGDGSYTKGTLALETLTIGEVV 245
           FDPADS+SF GVSCGS +CDRLEN  GC+AGRCRYEV YGDGSYTKGTLALETLT+G+V+
Sbjct: 190 FDPADSSSFAGVSCGSDVCDRLEN-TGCNAGRCRYEVSYGDGSYTKGTLALETLTVGQVM 249

Query: 246 VRDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFVGQLGGQTGGAFSYCLVSRGSGSTGALE 305
           +RDVAIGCGHTNQGMFIGAAGLLGLGGGSMSF+GQLGGQTGGAFSYCLVSRG+GSTGALE
Sbjct: 250 IRDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFIGQLGGQTGGAFSYCLVSRGTGSTGALE 309

Query: 306 FGRGALPVGATWISLIRNPRAPSFYYIGLAGVGVGGVRVPVPEETYRLTEYGSNGVVMDT 365
           FGRGALPVGATWISLIRNPRAPSFYYIGLAG+GVGGVRV VPEET++LTEYG+NGVVMDT
Sbjct: 310 FGRGALPVGATWISLIRNPRAPSFYYIGLAGIGVGGVRVSVPEETFQLTEYGTNGVVMDT 369

Query: 366 GTAVTRLPTAAYEALRDSFTAQTANLPRAGAVSIFDTCYDLNGFESVRVPTVSFYFSDGP 425
           GTAVTR PTAAY A RDSFTAQT+NLPRA  VSIFDTCYDLNGFESVRVPTVSFYFSDGP
Sbjct: 370 GTAVTRFPTAAYVAFRDSFTAQTSNLPRAPGVSIFDTCYDLNGFESVRVPTVSFYFSDGP 429

Query: 426 VLTLPAKNFLIPVNGAGTFCFAFAPSPSGLSIIGNIQQEGIQISFDGANGFVGFGPNVC 463
           VLTLPA+NFLIPV+G GTFC AFAPSPSGLSIIGNIQQEGIQISFDGANGFVGFGPN+C
Sbjct: 430 VLTLPARNFLIPVDGGGTFCLAFAPSPSGLSIIGNIQQEGIQISFDGANGFVGFGPNIC 482

[Close]

BLAST of MS004338 vs. NCBI nr
Match: KAG6573756.1 (Protein ASPARTIC PROTEASE IN GUARD CELL 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 755.4 bits (1949), Expect = 2.9e-214
Identity = 382/483 (79.09%), Postives = 417/483 (86.34%), Query Frame = 0


Query: 1   KIPFPLL--FLLHLLIFSAAAAADFQFLNVKETKFDSNDDRILHNLNFSDGHRQVTGKKS 60
           K+ F LL  F + ++  S +++ +FQ+LN+K +KFD ND +ILH LNFS+GHR VTG+KS
Sbjct: 4   KLHFSLLLFFSVSVVFGSRSSSVEFQYLNIKSSKFDVNDSQILHTLNFSNGHRLVTGRKS 63

Query: 61  NSSSSTKFKLKLVHRDKLPPSHVHGRRHGFVERMKRDASRVAALVRRLSHGGADSR---- 120
           N    TKFKLKLVHRD+LP  H+HG+  GF ER+KRD  RVA LVRRLS    DS     
Sbjct: 64  N---HTKFKLKLVHRDELP--HIHGQHQGFDERIKRDVKRVATLVRRLSRYDTDSSGVQD 123

Query: 121 ---------------MEEGSGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQCQPCSQCYQQ 180
                          MEEGSGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQCQPC QCYQQ
Sbjct: 124 NNYKVANFGTGVISGMEEGSGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQCQPCRQCYQQ 183

Query: 181 SDPVFDPADSASFTGVSCGSALCDRLENDAGCHAGRCRYEVLYGDGSYTKGTLALETLTI 240
           SDPVF+PA+S S+TGVSCGSA+CDRLEN AGCHAGRCRYEV YGDGSYTKGTLALETLTI
Sbjct: 184 SDPVFNPAESTSYTGVSCGSAVCDRLEN-AGCHAGRCRYEVSYGDGSYTKGTLALETLTI 243

Query: 241 GEVVVRDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFVGQLGGQTGGAFSYCLVSRGSGST 300
           GEV VRD+AIGCGHTNQGMFIGAAGLLGLGGGSMSFVGQLGGQTGGAFSYCLVSRG GST
Sbjct: 244 GEVTVRDMAIGCGHTNQGMFIGAAGLLGLGGGSMSFVGQLGGQTGGAFSYCLVSRGIGST 303

Query: 301 GALEFGRGALPVGATWISLIRNPRAPSFYYIGLAGVGVGGVRVPVPEETYRLTEYGSNGV 360
           G LEFGRGA+PVGATWISL+RNP APSFYYIGLAGVGVGG++VP+PEET++LTEYG+NGV
Sbjct: 304 GTLEFGRGAMPVGATWISLVRNPNAPSFYYIGLAGVGVGGIKVPIPEETFQLTEYGTNGV 363

Query: 361 VMDTGTAVTRLPTAAYEALRDSFTAQTANLPRAGAVSIFDTCYDLNGFESVRVPTVSFYF 420
           VMDTGTAVTRLPTAAY A RDSFTAQT+NLPRA  VSIFDTCY+LNGFESVRVPTVSFYF
Sbjct: 364 VMDTGTAVTRLPTAAYVAFRDSFTAQTSNLPRAPGVSIFDTCYNLNGFESVRVPTVSFYF 423

Query: 421 SDGPVLTLPAKNFLIPVNGAGTFCFAFAPSPSGLSIIGNIQQEGIQISFDGANGFVGFGP 463
           SDGPVLTLPA NFLIPV+ AGTFCFAFAPSPSGLSIIGNIQQ GIQISFDGANGFVGFGP
Sbjct: 424 SDGPVLTLPANNFLIPVDSAGTFCFAFAPSPSGLSIIGNIQQGGIQISFDGANGFVGFGP 480

[Close]

BLAST of MS004338 vs. ExPASy Swiss-Prot
Match: Q9LHE3 (Protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Arabidopsis thaliana OX=3702 GN=ASPG2 PE=2 SV=1)

HSP 1 Score: 616.7 bits (1589), Expect = 2.1e-175
Identity = 315/481 (65.49%), Postives = 372/481 (77.34%), Query Frame = 0


Query: 2   IPFPLLFL---LHLLIFSAAAAADFQFLNVKETKFDSNDD-RILHNLNFSDGHRQVTGKK 61
           +P    FL   LHL   S+ +  DFQ ++V +            +N +FSD         
Sbjct: 3   LPLFFFFLHLHLHLSSSSSISFPDFQIIDVLQPPLTVTATLPDFNNTHFSD--------- 62

Query: 62  SNSSSSTKFKLKLVHRDKLPPSHVHGRRHGFVERMKRDASRVAALVRRLSH---GGADSR 121
               SS+K+ L+L+HRD+ P        H    RM+RD  RV+A++RR+S      +DSR
Sbjct: 63  ---ESSSKYTLRLLHRDRFPSVTYRNHHHRLHARMRRDTDRVSAILRRISGKVIPSSDSR 122

Query: 122 -------------MEEGSGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQCQPCSQCYQQSD 181
                        M++GSGEYFVRIGVGSPPR QYMVIDSGSD+VWVQCQPC  CY+QSD
Sbjct: 123 YEVNDFGSDIVSGMDQGSGEYFVRIGVGSPPRDQYMVIDSGSDMVWVQCQPCKLCYKQSD 182

Query: 182 PVFDPADSASFTGVSCGSALCDRLENDAGCHAGRCRYEVLYGDGSYTKGTLALETLTIGE 241
           PVFDPA S S+TGVSCGS++CDR+EN +GCH+G CRYEV+YGDGSYTKGTLALETLT  +
Sbjct: 183 PVFDPAKSGSYTGVSCGSSVCDRIEN-SGCHSGGCRYEVMYGDGSYTKGTLALETLTFAK 242

Query: 242 VVVRDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFVGQLGGQTGGAFSYCLVSRGSGSTGA 301
            VVR+VA+GCGH N+GMFIGAAGLLG+GGGSMSFVGQL GQTGGAF YCLVSRG+ STG+
Sbjct: 243 TVVRNVAMGCGHRNRGMFIGAAGLLGIGGGSMSFVGQLSGQTGGAFGYCLVSRGTDSTGS 302

Query: 302 LEFGRGALPVGATWISLIRNPRAPSFYYIGLAGVGVGGVRVPVPEETYRLTEYGSNGVVM 361
           L FGR ALPVGA+W+ L+RNPRAPSFYY+GL G+GVGGVR+P+P+  + LTE G  GVVM
Sbjct: 303 LVFGREALPVGASWVPLVRNPRAPSFYYVGLKGLGVGGVRIPLPDGVFDLTETGDGGVVM 362

Query: 362 DTGTAVTRLPTAAYEALRDSFTAQTANLPRAGAVSIFDTCYDLNGFESVRVPTVSFYFSD 421
           DTGTAVTRLPTAAY A RD F +QTANLPRA  VSIFDTCYDL+GF SVRVPTVSFYF++
Sbjct: 363 DTGTAVTRLPTAAYVAFRDGFKSQTANLPRASGVSIFDTCYDLSGFVSVRVPTVSFYFTE 422

Query: 422 GPVLTLPAKNFLIPVNGAGTFCFAFAPSPSGLSIIGNIQQEGIQISFDGANGFVGFGPNV 463
           GPVLTLPA+NFL+PV+ +GT+CFAFA SP+GLSIIGNIQQEGIQ+SFDGANGFVGFGPNV
Sbjct: 423 GPVLTLPARNFLMPVDDSGTYCFAFAASPTGLSIIGNIQQEGIQVSFDGANGFVGFGPNV 470

[Close]

BLAST of MS004338 vs. ExPASy Swiss-Prot
Match: Q9LS40 (Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana OX=3702 GN=ASPG1 PE=1 SV=1)

HSP 1 Score: 375.2 bits (962), Expect = 1.1e-102
Identity = 193/430 (44.88%), Postives = 274/430 (63.72%), Query Frame = 0


Query: 61  SSSTKFKLKLVHRDKLPPSHVHGRRHGFVERMKRDASRVAALVRRLSHG--GAD------ 120
           +SS+   L+L  RD    S     +   + R++RD+SRVA +V ++     G D      
Sbjct: 75  NSSSPLSLELHSRDTFVASQHKDYKSLTLSRLERDSSRVAGIVAKIRFAVEGVDRSDLKP 134

Query: 121 ------------------SRMEEGSGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQCQPCS 180
                             S   +GSGEYF RIGVG+P +  Y+V+D+GSD+ W+QC+PC+
Sbjct: 135 VYNEDTRYQTEDLTTPVVSGASQGSGEYFSRIGVGTPAKEMYLVLDTGSDVNWIQCEPCA 194

Query: 181 QCYQQSDPVFDPADSASFTGVSCGSALCDRLENDAGCHAGRCRYEVLYGDGSYTKGTLAL 240
            CYQQSDPVF+P  S+++  ++C +  C  LE  A C + +C Y+V YGDGS+T G LA 
Sbjct: 195 DCYQQSDPVFNPTSSSTYKSLTCSAPQCSLLETSA-CRSNKCLYQVSYGDGSFTVGELAT 254

Query: 241 ETLTIGEV-VVRDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFVGQLGGQTGGAFSYCLVS 300
           +T+T G    + +VA+GCGH N+G+F GAAGLLGLGGG +S   Q+      +FSYCLV 
Sbjct: 255 DTVTFGNSGKINNVALGCGHDNEGLFTGAAGLLGLGGGVLSITNQM---KATSFSYCLVD 314

Query: 301 RGSGSTGALEFGRGALPVGATWISLIRNPRAPSFYYIGLAGVGVGGVRVPVPEETYRLTE 360
           R SG + +L+F    L  G     L+RN +  +FYY+GL+G  VGG +V +P+  + +  
Sbjct: 315 RDSGKSSSLDFNSVQLGGGDATAPLLRNKKIDTFYYVGLSGFSVGGEKVVLPDAIFDVDA 374

Query: 361 YGSNGVVMDTGTAVTRLPTAAYEALRDSFTAQTANLPR-AGAVSIFDTCYDLNGFESVRV 420
            GS GV++D GTAVTRL T AY +LRD+F   T NL + + ++S+FDTCYD +   +V+V
Sbjct: 375 SGSGGVILDCGTAVTRLQTQAYNSLRDAFLKLTVNLKKGSSSISLFDTCYDFSSLSTVKV 434

Query: 421 PTVSFYFSDGPVLTLPAKNFLIPVNGAGTFCFAFAPSPSGLSIIGNIQQEGIQISFDGAN 463
           PTV+F+F+ G  L LPAKN+LIPV+ +GTFCFAFAP+ S LSIIGN+QQ+G +I++D + 
Sbjct: 435 PTVAFHFTGGKSLDLPAKNYLIPVDDSGTFCFAFAPTSSSLSIIGNVQQQGTRITYDLSK 494

[Close]

BLAST of MS004338 vs. ExPASy Swiss-Prot
Match: Q9LNJ3 (Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 SV=1)

HSP 1 Score: 360.1 bits (923), Expect = 3.6e-98
Identity = 199/425 (46.82%), Postives = 269/425 (63.29%), Query Frame = 0


Query: 58  SNSSSSTKFKLKLVHRDKLPPSHVHGRRHGFVERMKRDASRVAALV--------RRLSH- 117
           S+S SS+   L L H D L  +        F  R++RD+ RV ++         R ++H 
Sbjct: 64  SDSESSSSITLNLDHIDALSSNKTPDEL--FSSRLQRDSRRVKSIATLAAQIPGRNVTHA 123

Query: 118 ---GGADSR----MEEGSGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQCQPCSQCYQQSD 177
              GG  S     + +GSGEYF R+GVG+P R  YMV+D+GSDIVW+QC PC +CY QSD
Sbjct: 124 PRPGGFSSSVVSGLSQGSGEYFTRLGVGTPARYVYMVLDTGSDIVWLQCAPCRRCYSQSD 183

Query: 178 PVFDPADSASFTGVSCGSALCDRLENDAGCHAGR--CRYEVLYGDGSYTKGTLALETLTI 237
           P+FDP  S ++  + C S  C RL++ AGC+  R  C Y+V YGDGS+T G  + ETLT 
Sbjct: 184 PIFDPRKSKTYATIPCSSPHCRRLDS-AGCNTRRKTCLYQVSYGDGSFTVGDFSTETLTF 243

Query: 238 GEVVVRDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFVGQLGGQTGGAFSYCLVSRGSGS- 297
               V+ VA+GCGH N+G+F+GAAGLLGLG G +SF GQ G +    FSYCLV R + S 
Sbjct: 244 RRNRVKGVALGCGHDNEGLFVGAAGLLGLGKGKLSFPGQTGHRFNQKFSYCLVDRSASSK 303

Query: 298 TGALEFGRGALPVGATWISLIRNPRAPSFYYIGLAGVGVGGVRVP-VPEETYRLTEYGSN 357
             ++ FG  A+   A +  L+ NP+  +FYY+GL G+ VGG RVP V    ++L + G+ 
Sbjct: 304 PSSVVFGNAAVSRIARFTPLLSNPKLDTFYYVGLLGISVGGTRVPGVTASLFKLDQIGNG 363

Query: 358 GVVMDTGTAVTRLPTAAYEALRDSFTAQTANLPRAGAVSIFDTCYDLNGFESVRVPTVSF 417
           GV++D+GT+VTRL   AY A+RD+F      L RA   S+FDTC+DL+    V+VPTV  
Sbjct: 364 GVIIDSGTSVTRLIRPAYIAMRDAFRVGAKTLKRAPDFSLFDTCFDLSNMNEVKVPTVVL 423

Query: 418 YFSDGPVLTLPAKNFLIPVNGAGTFCFAFAPSPSGLSIIGNIQQEGIQISFDGANGFVGF 463
           +F  G  ++LPA N+LIPV+  G FCFAFA +  GLSIIGNIQQ+G ++ +D A+  VGF
Sbjct: 424 HFR-GADVSLPATNYLIPVDTNGKFCFAFAGTMGGLSIIGNIQQQGFRVVYDLASSRVGF 483

[Close]

BLAST of MS004338 vs. ExPASy Swiss-Prot
Match: Q8S9J6 (Aspartyl protease family protein At5g10770 OS=Arabidopsis thaliana OX=3702 GN=At5g10770 PE=2 SV=1)

HSP 1 Score: 270.0 bits (689), Expect = 4.9e-71
Identity = 164/429 (38.23%), Postives = 229/429 (53.38%), Query Frame = 0


Query: 58  SNSSSSTKFKLKLVHRDKLPPSHVHGRRHG--FVERMKRDASRVAALVRRLSHGGADSRM 117
           S  +S+TK  L + HR        +G+      VE ++ D +RV ++  +LS   A   +
Sbjct: 52  SPRASTTKSSLHVTHRHGTCSRLNNGKATSPDHVEILRLDQARVNSIHSKLSKKLATDHV 111

Query: 118 EE--------------GSGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQCQPCSQ-CYQQS 177
            E              GSG Y V +G+G+P     ++ D+GSD+ W QCQPC + CY Q 
Sbjct: 112 SESKSTDLPAKDGSTLGSGNYIVTVGLGTPKNDLSLIFDTGSDLTWTQCQPCVRTCYDQK 171

Query: 178 DPVFDPADSASFTGVSCGSALCDRLENDAG----CHAGRCRYEVLYGDGSYTKGTLALET 237
           +P+F+P+ S S+  VSC SA C  L +  G    C A  C Y + YGD S++ G LA E 
Sbjct: 172 EPIFNPSKSTSYYNVSCSSAACGSLSSATGNAGSCSASNCIYGIQYGDQSFSVGFLAKEK 231

Query: 238 LTIGEVVVRD-VAIGCGHTNQGMFIGAAGLLGLGGGSMSFVGQLGGQTGGAFSYCLVSRG 297
            T+    V D V  GCG  NQG+F G AGLLGLG   +SF  Q        FSYCL S  
Sbjct: 232 FTLTNSDVFDGVYFGCGENNQGLFTGVAGLLGLGRDKLSFPSQTATAYNKIFSYCLPSSA 291

Query: 298 SGSTGALEFGRGALPVGATWISLIRNPRAPSFYYIGLAGVGVGGVRVPVPEETYRLTEYG 357
           S  TG L FG   +     +  +       SFY + +  + VGG ++P+P      T + 
Sbjct: 292 S-YTGHLTFGSAGISRSVKFTPISTITDGTSFYGLNIVAITVGGQKLPIPS-----TVFS 351

Query: 358 SNGVVMDTGTAVTRLPTAAYEALRDSFTAQTANLPRAGAVSIFDTCYDLNGFESVRVPTV 417
           + G ++D+GT +TRLP  AY ALR SF A+ +  P    VSI DTC+DL+GF++V +P V
Sbjct: 352 TPGALIDSGTVITRLPPKAYAALRSSFKAKMSKYPTTSGVSILDTCFDLSGFKTVTIPKV 411

Query: 418 SFYFSDGPVLTLPAKNFLIPVNGAGTFCFAFA--PSPSGLSIIGNIQQEGIQISFDGANG 463
           +F FS G V+ L +K  +  V      C AFA     S  +I GN+QQ+ +++ +DGA G
Sbjct: 412 AFSFSGGAVVELGSKG-IFYVFKISQVCLAFAGNSDDSNAAIFGNVQQQTLEVVYDGAGG 471

[Close]

BLAST of MS004338 vs. ExPASy Swiss-Prot
Match: Q9LEW3 (Aspartyl protease AED1 OS=Arabidopsis thaliana OX=3702 GN=AED1 PE=2 SV=1)

HSP 1 Score: 258.1 bits (658), Expect = 1.9e-67
Identity = 150/420 (35.71%), Postives = 217/420 (51.67%), Query Frame = 0


Query: 58  SNSSSSTKFKLKLVHRDKLPPSHVHGRRHGFVERMKRDASRVAALVRRLSHGGADSRMEE 117
           S+ +S+TK  L++VH            R    E ++RD +RV ++  +LS   A+   E 
Sbjct: 55  SSKASNTKSSLRVVHMHGACSHLSSDARVDHDEIIRRDQARVESIYSKLSKNSANEVSEA 114

Query: 118 -------------GSGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQCQPC-SQCYQQSDPV 177
                        GSG Y V IG+G+P     +V D+GSD+ W QC+PC   CY Q +P 
Sbjct: 115 KSTELPAKSGITLGSGNYIVTIGIGTPKHDLSLVFDTGSDLTWTQCEPCLGSCYSQKEPK 174

Query: 178 FDPADSASFTGVSCGSALCDRLENDAGCHAGRCRYEVLYGDGSYTKGTLALETLTI-GEV 237
           F+P+ S+++  VSC S +C+  E+   C A  C Y ++YGD S+T+G LA E  T+    
Sbjct: 175 FNPSSSSTYQNVSCSSPMCEDAES---CSASNCVYSIVYGDKSFTQGFLAKEKFTLTNSD 234

Query: 238 VVRDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFVGQLGGQTGGAFSYCLVSRGSGSTGAL 297
           V+ DV  GCG  NQG+F G AGLLGLG G +S   Q        FSYCL S  S STG L
Sbjct: 235 VLEDVYFGCGENNQGLFDGVAGLLGLGPGKLSLPAQTTTTYNNIFSYCLPSFTSNSTGHL 294

Query: 298 EFGRGALPVGATWISLIRNPRAPSFYYIGLAGVGVGGVRVPVPEETYRLTEYGSNGVVMD 357
            FG   +     +  +   P A + Y I + G+ VG   + +   +     + + G ++D
Sbjct: 295 TFGSAGISESVKFTPISSFPSAFN-YGIDIIGISVGDKELAITPNS-----FSTEGAIID 354

Query: 358 TGTAVTRLPTAAYEALRDSFTAQTANLPRAGAVSIFDTCYDLNGFESVRVPTVSFYFSDG 417
           +GT  TRLPT  Y  LR  F  + ++        +FDTCYD  G ++V  PT++F F+  
Sbjct: 355 SGTVFTRLPTKVYAELRSVFKEKMSSYKSTSGYGLFDTCYDFTGLDTVTYPTIAFSFAGS 414

Query: 418 PVLTLPAKNFLIPVNGAGTFCFAFAPSPSGLSIIGNIQQEGIQISFDGANGFVGFGPNVC 463
            V+ L      +P+      C AFA +    +I GN+QQ  + + +D A G VGF PN C
Sbjct: 415 TVVELDGSGISLPIK-ISQVCLAFAGNDDLPAIFGNVQQTTLDVVYDVAGGRVGFAPNGC 464

[Close]

BLAST of MS004338 vs. ExPASy TrEMBL
Match: A0A1S3BEG7 (protein ASPARTIC PROTEASE IN GUARD CELL 2-like OS=Cucumis melo OX=3656 GN=LOC103488783 PE=4 SV=1)

HSP 1 Score: 757.7 bits (1955), Expect = 2.9e-215
Identity = 387/478 (80.96%), Postives = 418/478 (87.45%), Query Frame = 0


Query: 6   LLFLLHLLIFSAAAA-----ADFQFLNVKETKFDSNDDRILHNLNFSDGHRQVTGKKSNS 65
           LLF L +L+  AAAA       FQ+LNVK TK D ND +ILH LNFSD HRQV+G KS++
Sbjct: 10  LLFFLAVLVVVAAAARPSSPTKFQYLNVKATKLDFNDGQILHTLNFSDAHRQVSGHKSHN 69

Query: 66  SSSTKFKLKLVHRDKLPPSHVHGRRHGFVERMKRDASRVAALVRRLSHGGA---DSR--- 125
            +   FKL L+HRDKL  SHVHG R GF +RMKRDA RVA LVRRLSHG A   DSR   
Sbjct: 70  DT---FKLNLLHRDKL--SHVHGHRRGFNDRMKRDAIRVATLVRRLSHGAAAVEDSRFKV 129

Query: 126 ----------MEEGSGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQCQPCSQCYQQSDPVF 185
                     MEEGSGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQC+PCS+CYQQSDPVF
Sbjct: 130 ANFATDVISGMEEGSGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQCKPCSRCYQQSDPVF 189

Query: 186 DPADSASFTGVSCGSALCDRLENDAGCHAGRCRYEVLYGDGSYTKGTLALETLTIGEVVV 245
           DPADS+SF GVSCGS +CDRLEN  GC+AGRCRYEV YGDGSYTKGTLALETLT+G+V++
Sbjct: 190 DPADSSSFAGVSCGSDVCDRLEN-TGCNAGRCRYEVSYGDGSYTKGTLALETLTVGQVMI 249

Query: 246 RDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFVGQLGGQTGGAFSYCLVSRGSGSTGALEF 305
           RDVAIGCGHTNQGMFIGAAGLLGLGGGSMSF+GQLGGQTGGAFSYCLVSRG+GSTG LEF
Sbjct: 250 RDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFIGQLGGQTGGAFSYCLVSRGTGSTGTLEF 309

Query: 306 GRGALPVGATWISLIRNPRAPSFYYIGLAGVGVGGVRVPVPEETYRLTEYGSNGVVMDTG 365
           GRGALPVGATWISLIRNPRAPSFYYIGLAG+GVGGVRV +PEET++LTE+G+NGVVMDTG
Sbjct: 310 GRGALPVGATWISLIRNPRAPSFYYIGLAGIGVGGVRVSIPEETFQLTEFGTNGVVMDTG 369

Query: 366 TAVTRLPTAAYEALRDSFTAQTANLPRAGAVSIFDTCYDLNGFESVRVPTVSFYFSDGPV 425
           TAVTRLPT+AY ALRDSFTAQT+NLPRA  VSIFDTCYDLNGFESVRVPTVSFYFSDGP 
Sbjct: 370 TAVTRLPTSAYVALRDSFTAQTSNLPRAPGVSIFDTCYDLNGFESVRVPTVSFYFSDGPA 429

Query: 426 LTLPAKNFLIPVNGAGTFCFAFAPSPSGLSIIGNIQQEGIQISFDGANGFVGFGPNVC 463
           LTLPAKNFLIPV+G GTFC AFAPSPSGLSIIGNIQQEGIQISFDGANGFVGFGPN+C
Sbjct: 430 LTLPAKNFLIPVDGGGTFCLAFAPSPSGLSIIGNIQQEGIQISFDGANGFVGFGPNIC 481

[Close]

BLAST of MS004338 vs. ExPASy TrEMBL
Match: A0A5A7SVY2 (Protein ASPARTIC PROTEASE IN GUARD CELL 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold65G00740 PE=4 SV=1)

HSP 1 Score: 756.9 bits (1953), Expect = 4.9e-215
Identity = 384/465 (82.58%), Postives = 416/465 (89.46%), Query Frame = 0


Query: 6   LLFLLHLLIFSAAAA-----ADFQFLNVKETKFDSNDDRILHNLNFSDGHRQVTGKKSNS 65
           LLF L +L+  AAAA       FQ+LNVK TK D ND +ILH LNFSD HRQV+G KS++
Sbjct: 10  LLFFLAVLVVVAAAARPSSPTKFQYLNVKATKLDFNDGQILHTLNFSDAHRQVSGHKSHN 69

Query: 66  SSSTKFKLKLVHRDKLPPSHVHGRRHGFVERMKRDASRVAALVRRLSHGGA---DSRMEE 125
            +   FKL L+HRDKL  SHVHG R GF +RMKRDA RVA LVRRLSHG A   DSR  +
Sbjct: 70  DT---FKLNLLHRDKL--SHVHGHRRGFNDRMKRDAIRVATLVRRLSHGAAAVEDSRF-K 129

Query: 126 GSGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQCQPCSQCYQQSDPVFDPADSASFTGVSC 185
           GSGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQC+PCS+CYQQSDPVFDPADS+SF GVSC
Sbjct: 130 GSGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQCKPCSRCYQQSDPVFDPADSSSFAGVSC 189

Query: 186 GSALCDRLENDAGCHAGRCRYEVLYGDGSYTKGTLALETLTIGEVVVRDVAIGCGHTNQG 245
           GS +CDRLEN  GC+AGRCRYEV YGDGSYTKGTLALETLT+G+V++RDVAIGCGHTNQG
Sbjct: 190 GSDVCDRLEN-TGCNAGRCRYEVSYGDGSYTKGTLALETLTVGQVMIRDVAIGCGHTNQG 249

Query: 246 MFIGAAGLLGLGGGSMSFVGQLGGQTGGAFSYCLVSRGSGSTGALEFGRGALPVGATWIS 305
           MFIGAAGLLGLGGGSMSF+GQLGGQTGGAFSYCLVSRG+GSTG LEFGRGALPVGATWIS
Sbjct: 250 MFIGAAGLLGLGGGSMSFIGQLGGQTGGAFSYCLVSRGTGSTGTLEFGRGALPVGATWIS 309

Query: 306 LIRNPRAPSFYYIGLAGVGVGGVRVPVPEETYRLTEYGSNGVVMDTGTAVTRLPTAAYEA 365
           LIRNPRAPSFYYIGLAG+GVGGVRV +PEET++LTE+G+NGVVMDTGTAVTRLPT+AY A
Sbjct: 310 LIRNPRAPSFYYIGLAGIGVGGVRVSIPEETFQLTEFGTNGVVMDTGTAVTRLPTSAYVA 369

Query: 366 LRDSFTAQTANLPRAGAVSIFDTCYDLNGFESVRVPTVSFYFSDGPVLTLPAKNFLIPVN 425
           LRDSFTAQT+NLPRA  VSIFDTCYDLNGFESVRVPTVSFYFSDGP LTLPAKNFLIPV+
Sbjct: 370 LRDSFTAQTSNLPRAPGVSIFDTCYDLNGFESVRVPTVSFYFSDGPALTLPAKNFLIPVD 429

Query: 426 GAGTFCFAFAPSPSGLSIIGNIQQEGIQISFDGANGFVGFGPNVC 463
           G GTFC AFAPSPSGLSIIGNIQQEGIQISFDGANGFVGFGPN+C
Sbjct: 430 GGGTFCLAFAPSPSGLSIIGNIQQEGIQISFDGANGFVGFGPNIC 467

[Close]

BLAST of MS004338 vs. ExPASy TrEMBL
Match: A0A0A0KPR4 (Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G579060 PE=4 SV=1)

HSP 1 Score: 756.1 bits (1951), Expect = 8.4e-215
Identity = 387/479 (80.79%), Postives = 420/479 (87.68%), Query Frame = 0


Query: 6   LLFLLHLLIFSAAAA-----ADFQFLNVKETKFDSNDDRILHNLNFSDGHRQVTGKKSNS 65
           LLFLL +++ +AAAA       FQ+LNVK TK D ND +ILH LNFSDGHRQV+G KS++
Sbjct: 10  LLFLLVVIVGAAAAARLSSSTKFQYLNVKATKLDFNDGQILHALNFSDGHRQVSGYKSDN 69

Query: 66  SSSTKFKLKLVHRDKLPPSHVHGRRHGFVERMKRDASRVAALVRRLSHGG----ADSR-- 125
           ++   FKL L+HRDKL  SHVHG R GF +RMKRDA RVA LVRRLSHG      DSR  
Sbjct: 70  NT---FKLNLLHRDKL--SHVHGHRRGFNDRMKRDAIRVATLVRRLSHGAPAAVKDSRYK 129

Query: 126 -----------MEEGSGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQCQPCSQCYQQSDPV 185
                      ME GSGEYFVRIGVGSPPR+QYMVIDSGSDIVWVQC+PCS+CYQQSDPV
Sbjct: 130 VANFATDVISGMEAGSGEYFVRIGVGSPPRNQYMVIDSGSDIVWVQCKPCSRCYQQSDPV 189

Query: 186 FDPADSASFTGVSCGSALCDRLENDAGCHAGRCRYEVLYGDGSYTKGTLALETLTIGEVV 245
           FDPADS+SF GVSCGS +CDRLEN  GC+AGRCRYEV YGDGSYTKGTLALETLT+G+V+
Sbjct: 190 FDPADSSSFAGVSCGSDVCDRLEN-TGCNAGRCRYEVSYGDGSYTKGTLALETLTVGQVM 249

Query: 246 VRDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFVGQLGGQTGGAFSYCLVSRGSGSTGALE 305
           +RDVAIGCGHTNQGMFIGAAGLLGLGGGSMSF+GQLGGQTGGAFSYCLVSRG+GSTGALE
Sbjct: 250 IRDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFIGQLGGQTGGAFSYCLVSRGTGSTGALE 309

Query: 306 FGRGALPVGATWISLIRNPRAPSFYYIGLAGVGVGGVRVPVPEETYRLTEYGSNGVVMDT 365
           FGRGALPVGATWISLIRNPRAPSFYYIGLAG+GVGGVRV VPEET++LTEYG+NGVVMDT
Sbjct: 310 FGRGALPVGATWISLIRNPRAPSFYYIGLAGIGVGGVRVSVPEETFQLTEYGTNGVVMDT 369

Query: 366 GTAVTRLPTAAYEALRDSFTAQTANLPRAGAVSIFDTCYDLNGFESVRVPTVSFYFSDGP 425
           GTAVTR PTAAY A RDSFTAQT+NLPRA  VSIFDTCYDLNGFESVRVPTVSFYFSDGP
Sbjct: 370 GTAVTRFPTAAYVAFRDSFTAQTSNLPRAPGVSIFDTCYDLNGFESVRVPTVSFYFSDGP 429

Query: 426 VLTLPAKNFLIPVNGAGTFCFAFAPSPSGLSIIGNIQQEGIQISFDGANGFVGFGPNVC 463
           VLTLPA+NFLIPV+G GTFC AFAPSPSGLSIIGNIQQEGIQISFDGANGFVGFGPN+C
Sbjct: 430 VLTLPARNFLIPVDGGGTFCLAFAPSPSGLSIIGNIQQEGIQISFDGANGFVGFGPNIC 482

[Close]

BLAST of MS004338 vs. ExPASy TrEMBL
Match: A0A6J1HQG0 (protein ASPARTIC PROTEASE IN GUARD CELL 2-like OS=Cucurbita maxima OX=3661 GN=LOC111466434 PE=4 SV=1)

HSP 1 Score: 751.9 bits (1940), Expect = 1.6e-213
Identity = 380/483 (78.67%), Postives = 416/483 (86.13%), Query Frame = 0


Query: 1   KIPFPLL--FLLHLLIFSAAAAADFQFLNVKETKFDSNDDRILHNLNFSDGHRQVTGKKS 60
           K+ F LL  F + +++ S +++ +FQ+LNVK  KFD ND ++LH LNFS+GHR VTG+KS
Sbjct: 4   KLHFSLLLFFSVSVVLGSRSSSVEFQYLNVKSNKFDVNDSQLLHTLNFSNGHRLVTGRKS 63

Query: 61  NSSSSTKFKLKLVHRDKLPPSHVHGRRHGFVERMKRDASRVAALVRRLSHG--------- 120
           N    TKFKLKLVHRDKLP  H+HG+  GF ER+KRD  RVA LVRRLS           
Sbjct: 64  N---HTKFKLKLVHRDKLP--HIHGQHQGFDERIKRDVKRVATLVRRLSRDDTTTSVVED 123

Query: 121 ----------GADSRMEEGSGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQCQPCSQCYQQ 180
                     G  S MEEGSGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQCQPC QCYQQ
Sbjct: 124 NSYKVANFGTGVISGMEEGSGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQCQPCRQCYQQ 183

Query: 181 SDPVFDPADSASFTGVSCGSALCDRLENDAGCHAGRCRYEVLYGDGSYTKGTLALETLTI 240
           SDPVF+PA+S S+TGVSCGSA+CDRLEN AGCH+GRCRYEV YGDGSYTKGTLALETLTI
Sbjct: 184 SDPVFNPAESTSYTGVSCGSAICDRLEN-AGCHSGRCRYEVSYGDGSYTKGTLALETLTI 243

Query: 241 GEVVVRDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFVGQLGGQTGGAFSYCLVSRGSGST 300
           GEV VRD+AIGCGHTNQGMFIGAAGLLGLGGGSMSFVGQLGGQTGGAFSYCLVSRG GST
Sbjct: 244 GEVTVRDMAIGCGHTNQGMFIGAAGLLGLGGGSMSFVGQLGGQTGGAFSYCLVSRGIGST 303

Query: 301 GALEFGRGALPVGATWISLIRNPRAPSFYYIGLAGVGVGGVRVPVPEETYRLTEYGSNGV 360
           G L+FGRGA+PVGATWISL+RNP APSFYYIGLAGVGVGG++VP+PEET++LTEYG+NGV
Sbjct: 304 GTLDFGRGAMPVGATWISLVRNPNAPSFYYIGLAGVGVGGIKVPIPEETFQLTEYGTNGV 363

Query: 361 VMDTGTAVTRLPTAAYEALRDSFTAQTANLPRAGAVSIFDTCYDLNGFESVRVPTVSFYF 420
           VMDTGTAVTRLPTAAY A RDSFTA T+NLPRA  VSIFDTCY+LNGFESVRVPTVSFYF
Sbjct: 364 VMDTGTAVTRLPTAAYVAFRDSFTALTSNLPRAPGVSIFDTCYNLNGFESVRVPTVSFYF 423

Query: 421 SDGPVLTLPAKNFLIPVNGAGTFCFAFAPSPSGLSIIGNIQQEGIQISFDGANGFVGFGP 463
           SDGPVLTLPA NFLIPV+ AGTFCFAFAPSPSGLSIIGNIQQ GIQISFDGANGFVGFGP
Sbjct: 424 SDGPVLTLPANNFLIPVDSAGTFCFAFAPSPSGLSIIGNIQQGGIQISFDGANGFVGFGP 480

[Close]

BLAST of MS004338 vs. ExPASy TrEMBL
Match: A0A6J1G1D0 (protein ASPARTIC PROTEASE IN GUARD CELL 2-like OS=Cucurbita moschata OX=3662 GN=LOC111449773 PE=4 SV=1)

HSP 1 Score: 751.9 bits (1940), Expect = 1.6e-213
Identity = 380/483 (78.67%), Postives = 417/483 (86.34%), Query Frame = 0


Query: 1   KIPFPLL--FLLHLLIFSAAAAADFQFLNVKETKFDSNDDRILHNLNFSDGHRQVTGKKS 60
           K+ F LL  F + ++  S ++  +FQ+LNVK +KFD+ND +ILH LNFS+GHR VTG+KS
Sbjct: 4   KLHFSLLLFFSVSVVFGSRSSPVEFQYLNVKSSKFDANDSQILHILNFSNGHRLVTGRKS 63

Query: 61  NSSSSTKFKLKLVHRDKLPPSHVHGRRHGFVERMKRDASRVAALVRRLSHGGADSR---- 120
           N    TKFKLKLVHRD+LP  H+HG+  GF ER+KRD  RVA LVRRLS    DS     
Sbjct: 64  N---HTKFKLKLVHRDELP--HIHGQHQGFDERIKRDVKRVATLVRRLSRYDTDSSGVQD 123

Query: 121 ---------------MEEGSGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQCQPCSQCYQQ 180
                          MEEG+GEYFVRIGVGSPPRSQYMVIDSGSDIVWVQCQPC QCYQQ
Sbjct: 124 NNYKVANFGTGVISGMEEGNGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQCQPCRQCYQQ 183

Query: 181 SDPVFDPADSASFTGVSCGSALCDRLENDAGCHAGRCRYEVLYGDGSYTKGTLALETLTI 240
           SDPVF+PA+S S+TGVSCGSA+CDRLEN AGCHAGRCRYEV YGDGSYTKGTLALETLTI
Sbjct: 184 SDPVFNPAESTSYTGVSCGSAVCDRLEN-AGCHAGRCRYEVSYGDGSYTKGTLALETLTI 243

Query: 241 GEVVVRDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFVGQLGGQTGGAFSYCLVSRGSGST 300
           GEV++ D+AIGCGHTNQGMFIGAAGLLGLGGGSMSFVGQLGGQTGGAFSYCLVSRG GST
Sbjct: 244 GEVMIHDMAIGCGHTNQGMFIGAAGLLGLGGGSMSFVGQLGGQTGGAFSYCLVSRGIGST 303

Query: 301 GALEFGRGALPVGATWISLIRNPRAPSFYYIGLAGVGVGGVRVPVPEETYRLTEYGSNGV 360
           G LEFGRGA+PVGATWISL+RNP APSFYYIGLAGVGVGG++VP+PEET++LTEYG+NGV
Sbjct: 304 GTLEFGRGAMPVGATWISLVRNPNAPSFYYIGLAGVGVGGIKVPIPEETFQLTEYGTNGV 363

Query: 361 VMDTGTAVTRLPTAAYEALRDSFTAQTANLPRAGAVSIFDTCYDLNGFESVRVPTVSFYF 420
           VMDTGTAVTRLPTAAY A RDSFTAQT+NLPRA  VSIFDTCY+LNGFESVRVPTVSFYF
Sbjct: 364 VMDTGTAVTRLPTAAYVAFRDSFTAQTSNLPRAPGVSIFDTCYNLNGFESVRVPTVSFYF 423

Query: 421 SDGPVLTLPAKNFLIPVNGAGTFCFAFAPSPSGLSIIGNIQQEGIQISFDGANGFVGFGP 463
           SDGPVLTLPA NFLIPV+ AGTFCFAFAPSPSGLSIIGNIQQ GIQISFDGANGFVGFGP
Sbjct: 424 SDGPVLTLPANNFLIPVDSAGTFCFAFAPSPSGLSIIGNIQQGGIQISFDGANGFVGFGP 480

[Close]

BLAST of MS004338 vs. TAIR 10
Match: AT3G20015.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 616.7 bits (1589), Expect = 1.5e-176
Identity = 315/481 (65.49%), Postives = 372/481 (77.34%), Query Frame = 0


Query: 2   IPFPLLFL---LHLLIFSAAAAADFQFLNVKETKFDSNDD-RILHNLNFSDGHRQVTGKK 61
           +P    FL   LHL   S+ +  DFQ ++V +            +N +FSD         
Sbjct: 3   LPLFFFFLHLHLHLSSSSSISFPDFQIIDVLQPPLTVTATLPDFNNTHFSD--------- 62

Query: 62  SNSSSSTKFKLKLVHRDKLPPSHVHGRRHGFVERMKRDASRVAALVRRLSH---GGADSR 121
               SS+K+ L+L+HRD+ P        H    RM+RD  RV+A++RR+S      +DSR
Sbjct: 63  ---ESSSKYTLRLLHRDRFPSVTYRNHHHRLHARMRRDTDRVSAILRRISGKVIPSSDSR 122

Query: 122 -------------MEEGSGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQCQPCSQCYQQSD 181
                        M++GSGEYFVRIGVGSPPR QYMVIDSGSD+VWVQCQPC  CY+QSD
Sbjct: 123 YEVNDFGSDIVSGMDQGSGEYFVRIGVGSPPRDQYMVIDSGSDMVWVQCQPCKLCYKQSD 182

Query: 182 PVFDPADSASFTGVSCGSALCDRLENDAGCHAGRCRYEVLYGDGSYTKGTLALETLTIGE 241
           PVFDPA S S+TGVSCGS++CDR+EN +GCH+G CRYEV+YGDGSYTKGTLALETLT  +
Sbjct: 183 PVFDPAKSGSYTGVSCGSSVCDRIEN-SGCHSGGCRYEVMYGDGSYTKGTLALETLTFAK 242

Query: 242 VVVRDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFVGQLGGQTGGAFSYCLVSRGSGSTGA 301
            VVR+VA+GCGH N+GMFIGAAGLLG+GGGSMSFVGQL GQTGGAF YCLVSRG+ STG+
Sbjct: 243 TVVRNVAMGCGHRNRGMFIGAAGLLGIGGGSMSFVGQLSGQTGGAFGYCLVSRGTDSTGS 302

Query: 302 LEFGRGALPVGATWISLIRNPRAPSFYYIGLAGVGVGGVRVPVPEETYRLTEYGSNGVVM 361
           L FGR ALPVGA+W+ L+RNPRAPSFYY+GL G+GVGGVR+P+P+  + LTE G  GVVM
Sbjct: 303 LVFGREALPVGASWVPLVRNPRAPSFYYVGLKGLGVGGVRIPLPDGVFDLTETGDGGVVM 362

Query: 362 DTGTAVTRLPTAAYEALRDSFTAQTANLPRAGAVSIFDTCYDLNGFESVRVPTVSFYFSD 421
           DTGTAVTRLPTAAY A RD F +QTANLPRA  VSIFDTCYDL+GF SVRVPTVSFYF++
Sbjct: 363 DTGTAVTRLPTAAYVAFRDGFKSQTANLPRASGVSIFDTCYDLSGFVSVRVPTVSFYFTE 422

Query: 422 GPVLTLPAKNFLIPVNGAGTFCFAFAPSPSGLSIIGNIQQEGIQISFDGANGFVGFGPNV 463
           GPVLTLPA+NFL+PV+ +GT+CFAFA SP+GLSIIGNIQQEGIQ+SFDGANGFVGFGPNV
Sbjct: 423 GPVLTLPARNFLMPVDDSGTYCFAFAASPTGLSIIGNIQQEGIQVSFDGANGFVGFGPNV 470

[Close]

BLAST of MS004338 vs. TAIR 10
Match: AT1G25510.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 380.9 bits (977), Expect = 1.4e-105
Identity = 195/447 (43.62%), Postives = 281/447 (62.86%), Query Frame = 0


Query: 41  LHNLNFSDGHRQVTGKKSNSSSSTKFKLKLVHRDKLPPSHVHGRRHGFVERMKRDASRVA 100
           +H   ++   R    ++   S+S+ F L+L  R  +  +     +   + R+ RD +RV 
Sbjct: 42  IHRTKYTSSFRLNQQEEQTHSASSSFSLQLHSRVSVRGTEHSDYKSLTLARLNRDTARVK 101

Query: 101 ALVRRL-----SHGGAD--------------------SRMEEGSGEYFVRIGVGSPPRSQ 160
           +L+ RL     +   AD                    S   +GSGEYF R+G+G P R  
Sbjct: 102 SLITRLDLAINNISKADLKPISTMYTTEEQDIEAPLISGTTQGSGEYFTRVGIGKPAREV 161

Query: 161 YMVIDSGSDIVWVQCQPCSQCYQQSDPVFDPADSASFTGVSCGSALCDRLENDAGCHAGR 220
           YMV+D+GSD+ W+QC PC+ CY Q++P+F+P+ S+S+  +SC +  C+ LE  + C    
Sbjct: 162 YMVLDTGSDVNWLQCTPCADCYHQTEPIFEPSSSSSYEPLSCDTPQCNALE-VSECRNAT 221

Query: 221 CRYEVLYGDGSYTKGTLALETLTIGEVVVRDVAIGCGHTNQGMFIGAAGLLGLGGGSMSF 280
           C YEV YGDGSYT G  A ETLTIG  +V++VA+GCGH+N+G+F+GAAGLLGLGGG ++ 
Sbjct: 222 CLYEVSYGDGSYTVGDFATETLTIGSTLVQNVAVGCGHSNEGLFVGAAGLLGLGGGLLAL 281

Query: 281 VGQLGGQTGGAFSYCLVSRGSGSTGALEFGRGALPVGATWISLIRNPRAPSFYYIGLAGV 340
             QL      +FSYCLV R S S   ++FG    P  A    L+RN +  +FYY+GL G+
Sbjct: 282 PSQLNTT---SFSYCLVDRDSDSASTVDFGTSLSP-DAVVAPLLRNHQLDTFYYLGLTGI 341

Query: 341 GVGGVRVPVPEETYRLTEYGSNGVVMDTGTAVTRLPTAAYEALRDSFTAQTANLPRAGAV 400
            VGG  + +P+ ++ + E GS G+++D+GTAVTRL T  Y +LRDSF   T +L +A  V
Sbjct: 342 SVGGELLQIPQSSFEMDESGSGGIIIDSGTAVTRLQTEIYNSLRDSFVKGTLDLEKAAGV 401

Query: 401 SIFDTCYDLNGFESVRVPTVSFYFSDGPVLTLPAKNFLIPVNGAGTFCFAFAPSPSGLSI 460
           ++FDTCY+L+   +V VPTV+F+F  G +L LPAKN++IPV+  GTFC AFAP+ S L+I
Sbjct: 402 AMFDTCYNLSAKTTVEVPTVAFHFPGGKMLALPAKNYMIPVDSVGTFCLAFAPTASSLAI 461

Query: 461 IGNIQQEGIQISFDGANGFVGFGPNVC 463
           IGN+QQ+G +++FD AN  +GF  N C
Sbjct: 462 IGNVQQQGTRVTFDLANSLIGFSSNKC 483

[Close]

BLAST of MS004338 vs. TAIR 10
Match: AT3G18490.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 375.2 bits (962), Expect = 7.7e-104
Identity = 193/430 (44.88%), Postives = 274/430 (63.72%), Query Frame = 0


Query: 61  SSSTKFKLKLVHRDKLPPSHVHGRRHGFVERMKRDASRVAALVRRLSHG--GAD------ 120
           +SS+   L+L  RD    S     +   + R++RD+SRVA +V ++     G D      
Sbjct: 75  NSSSPLSLELHSRDTFVASQHKDYKSLTLSRLERDSSRVAGIVAKIRFAVEGVDRSDLKP 134

Query: 121 ------------------SRMEEGSGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQCQPCS 180
                             S   +GSGEYF RIGVG+P +  Y+V+D+GSD+ W+QC+PC+
Sbjct: 135 VYNEDTRYQTEDLTTPVVSGASQGSGEYFSRIGVGTPAKEMYLVLDTGSDVNWIQCEPCA 194

Query: 181 QCYQQSDPVFDPADSASFTGVSCGSALCDRLENDAGCHAGRCRYEVLYGDGSYTKGTLAL 240
            CYQQSDPVF+P  S+++  ++C +  C  LE  A C + +C Y+V YGDGS+T G LA 
Sbjct: 195 DCYQQSDPVFNPTSSSTYKSLTCSAPQCSLLETSA-CRSNKCLYQVSYGDGSFTVGELAT 254

Query: 241 ETLTIGEV-VVRDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFVGQLGGQTGGAFSYCLVS 300
           +T+T G    + +VA+GCGH N+G+F GAAGLLGLGGG +S   Q+      +FSYCLV 
Sbjct: 255 DTVTFGNSGKINNVALGCGHDNEGLFTGAAGLLGLGGGVLSITNQM---KATSFSYCLVD 314

Query: 301 RGSGSTGALEFGRGALPVGATWISLIRNPRAPSFYYIGLAGVGVGGVRVPVPEETYRLTE 360
           R SG + +L+F    L  G     L+RN +  +FYY+GL+G  VGG +V +P+  + +  
Sbjct: 315 RDSGKSSSLDFNSVQLGGGDATAPLLRNKKIDTFYYVGLSGFSVGGEKVVLPDAIFDVDA 374

Query: 361 YGSNGVVMDTGTAVTRLPTAAYEALRDSFTAQTANLPR-AGAVSIFDTCYDLNGFESVRV 420
            GS GV++D GTAVTRL T AY +LRD+F   T NL + + ++S+FDTCYD +   +V+V
Sbjct: 375 SGSGGVILDCGTAVTRLQTQAYNSLRDAFLKLTVNLKKGSSSISLFDTCYDFSSLSTVKV 434

Query: 421 PTVSFYFSDGPVLTLPAKNFLIPVNGAGTFCFAFAPSPSGLSIIGNIQQEGIQISFDGAN 463
           PTV+F+F+ G  L LPAKN+LIPV+ +GTFCFAFAP+ S LSIIGN+QQ+G +I++D + 
Sbjct: 435 PTVAFHFTGGKSLDLPAKNYLIPVDDSGTFCFAFAPTSSSLSIIGNVQQQGTRITYDLSK 494

[Close]

BLAST of MS004338 vs. TAIR 10
Match: AT1G01300.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 360.1 bits (923), Expect = 2.6e-99
Identity = 199/425 (46.82%), Postives = 269/425 (63.29%), Query Frame = 0


Query: 58  SNSSSSTKFKLKLVHRDKLPPSHVHGRRHGFVERMKRDASRVAALV--------RRLSH- 117
           S+S SS+   L L H D L  +        F  R++RD+ RV ++         R ++H 
Sbjct: 64  SDSESSSSITLNLDHIDALSSNKTPDEL--FSSRLQRDSRRVKSIATLAAQIPGRNVTHA 123

Query: 118 ---GGADSR----MEEGSGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQCQPCSQCYQQSD 177
              GG  S     + +GSGEYF R+GVG+P R  YMV+D+GSDIVW+QC PC +CY QSD
Sbjct: 124 PRPGGFSSSVVSGLSQGSGEYFTRLGVGTPARYVYMVLDTGSDIVWLQCAPCRRCYSQSD 183

Query: 178 PVFDPADSASFTGVSCGSALCDRLENDAGCHAGR--CRYEVLYGDGSYTKGTLALETLTI 237
           P+FDP  S ++  + C S  C RL++ AGC+  R  C Y+V YGDGS+T G  + ETLT 
Sbjct: 184 PIFDPRKSKTYATIPCSSPHCRRLDS-AGCNTRRKTCLYQVSYGDGSFTVGDFSTETLTF 243

Query: 238 GEVVVRDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFVGQLGGQTGGAFSYCLVSRGSGS- 297
               V+ VA+GCGH N+G+F+GAAGLLGLG G +SF GQ G +    FSYCLV R + S 
Sbjct: 244 RRNRVKGVALGCGHDNEGLFVGAAGLLGLGKGKLSFPGQTGHRFNQKFSYCLVDRSASSK 303

Query: 298 TGALEFGRGALPVGATWISLIRNPRAPSFYYIGLAGVGVGGVRVP-VPEETYRLTEYGSN 357
             ++ FG  A+   A +  L+ NP+  +FYY+GL G+ VGG RVP V    ++L + G+ 
Sbjct: 304 PSSVVFGNAAVSRIARFTPLLSNPKLDTFYYVGLLGISVGGTRVPGVTASLFKLDQIGNG 363

Query: 358 GVVMDTGTAVTRLPTAAYEALRDSFTAQTANLPRAGAVSIFDTCYDLNGFESVRVPTVSF 417
           GV++D+GT+VTRL   AY A+RD+F      L RA   S+FDTC+DL+    V+VPTV  
Sbjct: 364 GVIIDSGTSVTRLIRPAYIAMRDAFRVGAKTLKRAPDFSLFDTCFDLSNMNEVKVPTVVL 423

Query: 418 YFSDGPVLTLPAKNFLIPVNGAGTFCFAFAPSPSGLSIIGNIQQEGIQISFDGANGFVGF 463
           +F  G  ++LPA N+LIPV+  G FCFAFA +  GLSIIGNIQQ+G ++ +D A+  VGF
Sbjct: 424 HFR-GADVSLPATNYLIPVDTNGKFCFAFAGTMGGLSIIGNIQQQGFRVVYDLASSRVGF 483

[Close]

BLAST of MS004338 vs. TAIR 10
Match: AT3G61820.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 354.4 bits (908), Expect = 1.4e-97
Identity = 202/478 (42.26%), Postives = 281/478 (58.79%), Query Frame = 0


Query: 12  LLIFSAAAAADFQFLNVKETKFDSNDDRILHNLNFSDGHRQVTGKKSNSSSSTKFKLKLV 71
           +L F+++A++ +Q L V         + +  +   S    +    +S S S+T   + L 
Sbjct: 16  VLFFTSSASSQYQTLVV---------NTLPSSATLSWPESESLTDESLSESTTSLSVHLS 75

Query: 72  HRDKLPPSHVHGRRHGFVERMKRDASRVAALVRRLS------------------HGGADS 131
           H D L           F  R++RD+ RV ++    +                   G   S
Sbjct: 76  HVDALSSFSDASPADLFNLRLQRDSLRVKSITSLAAVSTGRNATKRTPRTAGGFSGAVIS 135

Query: 132 RMEEGSGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQCQPCSQCYQQSDPVFDPADSASFT 191
            + +GSGEYF+R+GVG+P  + YMV+D+GSD+VW+QC PC  CY Q+D +FDP  S +F 
Sbjct: 136 GLSQGSGEYFMRLGVGTPATNVYMVLDTGSDVVWLQCSPCKACYNQTDAIFDPKKSKTFA 195

Query: 192 GVSCGSALCDRLENDAGCHAGR---CRYEVLYGDGSYTKGTLALETLTIGEVVVRDVAIG 251
            V CGS LC RL++ + C   R   C Y+V YGDGS+T+G  + ETLT     V  V +G
Sbjct: 196 TVPCGSRLCRRLDDSSECVTRRSKTCLYQVSYGDGSFTEGDFSTETLTFHGARVDHVPLG 255

Query: 252 CGHTNQGMFIGAAGLLGLGGGSMSFVGQLGGQTGGAFSYCLVSR-GSGST----GALEFG 311
           CGH N+G+F+GAAGLLGLG G +SF  Q   +  G FSYCLV R  SGS+      + FG
Sbjct: 256 CGHDNEGLFVGAAGLLGLGRGGLSFPSQTKNRYNGKFSYCLVDRTSSGSSSKPPSTIVFG 315

Query: 312 RGALPVGATWISLIRNPRAPSFYYIGLAGVGVGGVRVP-VPEETYRLTEYGSNGVVMDTG 371
             A+P  + +  L+ NP+  +FYY+ L G+ VGG RVP V E  ++L   G+ GV++D+G
Sbjct: 316 NAAVPKTSVFTPLLTNPKLDTFYYLQLLGISVGGSRVPGVSESQFKLDATGNGGVIIDSG 375

Query: 372 TAVTRLPTAAYEALRDSFTAQTANLPRAGAVSIFDTCYDLNGFESVRVPTVSFYFSDGPV 431
           T+VTRL   AY ALRD+F      L RA + S+FDTC+DL+G  +V+VPTV F+F  G V
Sbjct: 376 TSVTRLTQPAYVALRDAFRLGATKLKRAPSYSLFDTCFDLSGMTTVKVPTVVFHFGGGEV 435

Query: 432 LTLPAKNFLIPVNGAGTFCFAFAPSPSGLSIIGNIQQEGIQISFDGANGFVGFGPNVC 463
            +LPA N+LIPVN  G FCFAFA +   LSIIGNIQQ+G ++++D     VGF    C
Sbjct: 436 -SLPASNYLIPVNTEGRFCFAFAGTMGSLSIIGNIQQQGFRVAYDLVGSRVGFLSRAC 483

The following BLAST results are available for this feature:

BLAST of MS004338 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038893071.1	1.7e-217	81.95	protein ASPARTIC PROTEASE IN GUARD CELL 2-like [Benincasa hispida]	[more]
XP_008445900.1	5.9e-215	80.96	PREDICTED: protein ASPARTIC PROTEASE IN GUARD CELL 2-like [Cucumis melo]	[more]
KAA0034086.1	1.0e-214	82.58	protein ASPARTIC PROTEASE IN GUARD CELL 2-like [Cucumis melo var. makuwa]	[more]
XP_004147103.1	1.7e-214	80.79	protein ASPARTIC PROTEASE IN GUARD CELL 2 [Cucumis sativus] >KGN51568.1 hypothet...	[more]
KAG6573756.1	2.9e-214	79.09	Protein ASPARTIC PROTEASE IN GUARD CELL 2, partial [Cucurbita argyrosperma subsp...	[more]

BLAST of MS004338 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LHE3	2.1e-175	65.49	Protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Arabidopsis thaliana OX=3702 GN=ASP...	[more]
Q9LS40	1.1e-102	44.88	Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana OX=3702 GN=ASP...	[more]
Q9LNJ3	3.6e-98	46.82	Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 ...	[more]
Q8S9J6	4.9e-71	38.23	Aspartyl protease family protein At5g10770 OS=Arabidopsis thaliana OX=3702 GN=At...	[more]
Q9LEW3	1.9e-67	35.71	Aspartyl protease AED1 OS=Arabidopsis thaliana OX=3702 GN=AED1 PE=2 SV=1	[more]

BLAST of MS004338 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3BEG7	2.9e-215	80.96	protein ASPARTIC PROTEASE IN GUARD CELL 2-like OS=Cucumis melo OX=3656 GN=LOC103...	[more]
A0A5A7SVY2	4.9e-215	82.58	Protein ASPARTIC PROTEASE IN GUARD CELL 2-like OS=Cucumis melo var. makuwa OX=11...	[more]
A0A0A0KPR4	8.4e-215	80.79	Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G57906...	[more]
A0A6J1HQG0	1.6e-213	78.67	protein ASPARTIC PROTEASE IN GUARD CELL 2-like OS=Cucurbita maxima OX=3661 GN=LO...	[more]
A0A6J1G1D0	1.6e-213	78.67	protein ASPARTIC PROTEASE IN GUARD CELL 2-like OS=Cucurbita moschata OX=3662 GN=...	[more]

BLAST of MS004338 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G20015.1	1.5e-176	65.49	Eukaryotic aspartyl protease family protein	[more]
AT1G25510.1	1.4e-105	43.62	Eukaryotic aspartyl protease family protein	[more]
AT3G18490.1	7.7e-104	44.88	Eukaryotic aspartyl protease family protein	[more]
AT1G01300.1	2.6e-99	46.82	Eukaryotic aspartyl protease family protein	[more]
AT3G61820.1	1.4e-97	42.26	Eukaryotic aspartyl protease family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR032861	Xylanase inhibitor, N-terminal	PFAM	PF14543	TAXi_N	coord: 122..285 e-value: 1.4E-48 score: 165.5
IPR021109	Aspartic peptidase domain superfamily	GENE3D	2.40.70.10	Acid Proteases	coord: 108..285 e-value: 3.8E-49 score: 169.3
IPR021109	Aspartic peptidase domain superfamily	GENE3D	2.40.70.10	Acid Proteases	coord: 292..462 e-value: 4.4E-44 score: 152.3
IPR021109	Aspartic peptidase domain superfamily	SUPERFAMILY	50630	Acid proteases	coord: 118..462
IPR032799	Xylanase inhibitor, C-terminal	PFAM	PF14541	TAXi_C	coord: 307..458 e-value: 3.6E-32 score: 111.4
None	No IPR available	PANTHER	PTHR13683:SF265	PROTEIN ASPARTIC PROTEASE IN GUARD CELL 2	coord: 51..462
IPR001461	Aspartic peptidase A1 family	PANTHER	PTHR13683	ASPARTYL PROTEASES	coord: 51..462
IPR033121	Peptidase family A1 domain	PROSITE	PS51767	PEPTIDASE_A1	coord: 122..458 score: 45.674072

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS004338.1	MS004338.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
molecular_function	GO:0004190	aspartic-type endopeptidase activity