MS004318 (gene) Bitter gourd (TR) v1

Overview
NameMS004318
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionTranscription termination factor MTERF5
Locationscaffold92: 1793629 .. 1794732 (-)
RNA-Seq ExpressionMS004318
SyntenyMS004318
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTAAAAATTCATCCAGAATTTTGCTATGTTTCGTTCAAAAACGTTTCATCAGCAAGGCTTCTGGTTCTACATTGCCTTCGTCTTCTTTGTATACGATTGAATTCCTCAAGAATTCTTGTGGGCTTGCTTCGGAATCTGCTCTCTCTGCCCATCGAAAGCTTCAACTCGACGAAAAAAACTCCCGTAAGTACGAATCGGTTCTTAATTTATTGAAATCGTATGGATTCGAGGGTTCGCAGATCGCTAAGTTGATCGCGAGGCGGCCTTCGATCCTCCAATCGAGAGTCGACAACAATCTGAAGCCTAAATTTGAGTTCCTCGAGGAAATTGGGTTCGTCGGTCCTTTACTTCCGAAGGTAATTATATCGAACCCTATAATTCTGCTCAGGGGCTTAGATTCTCAGCTGAAACCATCGGTTTGTTTCATTAAGGAAATGCTTGAATCGAATGAGAAAGTTATTGCCGCCATTCGTCGATCTTCATGGGTTTTAACTTTTGATTTTAAGGGTATAATGCAATCTAACATTGATGTTCTGCTCCGTGAAGGGGTGCCTTCCAGGAATATATCGAAATTGATTGTGGTTCAGCCAAGAACTATCATGCAGAAGGTTGATAGAATGATTTTTGCAGTGAATAAGGCCAAGGAATTGGGCATTGATCCAACGACCCTCATGTTTGTTCAAGCGGTTCGTGTAATGGTTTCCATGAACGACTCAACTTGGAAGAAGAAAATAAATGTTTTGAAGAGTTTGGGATGGTCTGAGAAGGAGATTTTAACAGCATTTAAGAGAGATCCATTCTGTTTGGCCTGTTCAGAGGAGAAAATGAGGGGTGTGGCAGATTTCTGTTTCAACACTGCAAAGTTGGACCCAGAAACTGTGATTTCTTACCCCAAATTCTTCATGTATGGACTCGACAAACGGCTCCGGCCGAGATATAAAGTTCTTGAGGTTTTGAAGGTGAAGAATCTGATTAAAAAGAACAGAAAGATTGCTTGGTTGCTAATCCAAGGGGAGAGAAGTTTCTTAAAGAATTATGTTCTTAAATATTTGGATAAAATCCCAGATCTGATGGATATATACCGCGGCAAAGCAGCTGCT

mRNA sequence

ATGGCTAAAAATTCATCCAGAATTTTGCTATGTTTCGTTCAAAAACGTTTCATCAGCAAGGCTTCTGGTTCTACATTGCCTTCGTCTTCTTTGTATACGATTGAATTCCTCAAGAATTCTTGTGGGCTTGCTTCGGAATCTGCTCTCTCTGCCCATCGAAAGCTTCAACTCGACGAAAAAAACTCCCGTAAGTACGAATCGGTTCTTAATTTATTGAAATCGTATGGATTCGAGGGTTCGCAGATCGCTAAGTTGATCGCGAGGCGGCCTTCGATCCTCCAATCGAGAGTCGACAACAATCTGAAGCCTAAATTTGAGTTCCTCGAGGAAATTGGGTTCGTCGGTCCTTTACTTCCGAAGGTAATTATATCGAACCCTATAATTCTGCTCAGGGGCTTAGATTCTCAGCTGAAACCATCGGTTTGTTTCATTAAGGAAATGCTTGAATCGAATGAGAAAGTTATTGCCGCCATTCGTCGATCTTCATGGGTTTTAACTTTTGATTTTAAGGGTATAATGCAATCTAACATTGATGTTCTGCTCCGTGAAGGGGTGCCTTCCAGGAATATATCGAAATTGATTGTGGTTCAGCCAAGAACTATCATGCAGAAGGTTGATAGAATGATTTTTGCAGTGAATAAGGCCAAGGAATTGGGCATTGATCCAACGACCCTCATGTTTGTTCAAGCGGTTCGTGTAATGGTTTCCATGAACGACTCAACTTGGAAGAAGAAAATAAATGTTTTGAAGAGTTTGGGATGGTCTGAGAAGGAGATTTTAACAGCATTTAAGAGAGATCCATTCTGTTTGGCCTGTTCAGAGGAGAAAATGAGGGGTGTGGCAGATTTCTGTTTCAACACTGCAAAGTTGGACCCAGAAACTGTGATTTCTTACCCCAAATTCTTCATGTATGGACTCGACAAACGGCTCCGGCCGAGATATAAAGTTCTTGAGGTTTTGAAGGTGAAGAATCTGATTAAAAAGAACAGAAAGATTGCTTGGTTGCTAATCCAAGGGGAGAGAAGTTTCTTAAAGAATTATGTTCTTAAATATTTGGATAAAATCCCAGATCTGATGGATATATACCGCGGCAAAGCAGCTGCT

Coding sequence (CDS)

ATGGCTAAAAATTCATCCAGAATTTTGCTATGTTTCGTTCAAAAACGTTTCATCAGCAAGGCTTCTGGTTCTACATTGCCTTCGTCTTCTTTGTATACGATTGAATTCCTCAAGAATTCTTGTGGGCTTGCTTCGGAATCTGCTCTCTCTGCCCATCGAAAGCTTCAACTCGACGAAAAAAACTCCCGTAAGTACGAATCGGTTCTTAATTTATTGAAATCGTATGGATTCGAGGGTTCGCAGATCGCTAAGTTGATCGCGAGGCGGCCTTCGATCCTCCAATCGAGAGTCGACAACAATCTGAAGCCTAAATTTGAGTTCCTCGAGGAAATTGGGTTCGTCGGTCCTTTACTTCCGAAGGTAATTATATCGAACCCTATAATTCTGCTCAGGGGCTTAGATTCTCAGCTGAAACCATCGGTTTGTTTCATTAAGGAAATGCTTGAATCGAATGAGAAAGTTATTGCCGCCATTCGTCGATCTTCATGGGTTTTAACTTTTGATTTTAAGGGTATAATGCAATCTAACATTGATGTTCTGCTCCGTGAAGGGGTGCCTTCCAGGAATATATCGAAATTGATTGTGGTTCAGCCAAGAACTATCATGCAGAAGGTTGATAGAATGATTTTTGCAGTGAATAAGGCCAAGGAATTGGGCATTGATCCAACGACCCTCATGTTTGTTCAAGCGGTTCGTGTAATGGTTTCCATGAACGACTCAACTTGGAAGAAGAAAATAAATGTTTTGAAGAGTTTGGGATGGTCTGAGAAGGAGATTTTAACAGCATTTAAGAGAGATCCATTCTGTTTGGCCTGTTCAGAGGAGAAAATGAGGGGTGTGGCAGATTTCTGTTTCAACACTGCAAAGTTGGACCCAGAAACTGTGATTTCTTACCCCAAATTCTTCATGTATGGACTCGACAAACGGCTCCGGCCGAGATATAAAGTTCTTGAGGTTTTGAAGGTGAAGAATCTGATTAAAAAGAACAGAAAGATTGCTTGGTTGCTAATCCAAGGGGAGAGAAGTTTCTTAAAGAATTATGTTCTTAAATATTTGGATAAAATCCCAGATCTGATGGATATATACCGCGGCAAAGCAGCTGCT

Protein sequence

MAKNSSRILLCFVQKRFISKASGSTLPSSSLYTIEFLKNSCGLASESALSAHRKLQLDEKNSRKYESVLNLLKSYGFEGSQIAKLIARRPSILQSRVDNNLKPKFEFLEEIGFVGPLLPKVIISNPIILLRGLDSQLKPSVCFIKEMLESNEKVIAAIRRSSWVLTFDFKGIMQSNIDVLLREGVPSRNISKLIVVQPRTIMQKVDRMIFAVNKAKELGIDPTTLMFVQAVRVMVSMNDSTWKKKINVLKSLGWSEKEILTAFKRDPFCLACSEEKMRGVADFCFNTAKLDPETVISYPKFFMYGLDKRLRPRYKVLEVLKVKNLIKKNRKIAWLLIQGERSFLKNYVLKYLDKIPDLMDIYRGKAAA
Homology
BLAST of MS004318 vs. NCBI nr
Match: XP_023529167.1 (transcription termination factor MTERF5, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 562.4 bits (1448), Expect = 2.9e-156
Identity = 289/368 (78.53%), Postives = 325/368 (88.32%), Query Frame = 0

Query: 1   MAKNSSRILLCFVQKRFISKASGSTLPSSSLYTIEFLKNSCGLASESALSAHRKLQLDEK 60
           M+K SS  LL F+Q RFI+  SGSTLP +S+ TIEFLKNSCGLA++S  SA RKLQ DEK
Sbjct: 1   MSKISSTFLLYFIQNRFINTISGSTLPLASVSTIEFLKNSCGLATDSPSSALRKLQFDEK 60

Query: 61  NSRKYESVLNLLKSYGFEGSQIAKLIARRPSILQSRVDNNLKPKFEFLEEIGFVGPLLPK 120
           +++KYE+V   LKSYGFE SQIAKLIA+RPSILQSRV +NLKPKFEFLEEIGFVGPLLPK
Sbjct: 61  SAKKYEAVPRFLKSYGFENSQIAKLIAKRPSILQSRVSSNLKPKFEFLEEIGFVGPLLPK 120

Query: 121 VIISNPIILLRGLDSQLKPSVCFIKEMLESNEKVIAAIRRSSWVLTFDFKGIMQSNIDVL 180
           VI+SNP IL RGLDSQLKPS CFIKEMLES+E+   AIRRSSW+LTFDFKG+MQSN++VL
Sbjct: 121 VILSNPTILQRGLDSQLKPSFCFIKEMLESDEQANFAIRRSSWLLTFDFKGVMQSNVNVL 180

Query: 181 LREGVPSRNISKLIVVQPRTIMQKVDRMIFAVNKAKELGIDPTTLMFVQAVRVMVSMNDS 240
           L EGVPSRNISKLI  QPRTIMQKVDRM+ AV K KELGI+P T MFV+A+RV++SMND+
Sbjct: 181 LGEGVPSRNISKLIGQQPRTIMQKVDRMVCAVKKVKELGIEPQTRMFVRALRVVLSMNDT 240

Query: 241 TWKKKINVLKSLGWSEKEILTAFKRDPFCLACSEEKMRGVADFCFNTAKLDPETVISYPK 300
           TWKKKINV KSLGWSEKEILTAFKRDP+CLACSEEKMR VADFCFN AKLDPETVISYP 
Sbjct: 241 TWKKKINVFKSLGWSEKEILTAFKRDPYCLACSEEKMRAVADFCFNAAKLDPETVISYPT 300

Query: 301 FFMYGLDKRLRPRYKVLEVLKVKNLIKKNRKIAWLLIQGERSFLKNYVLKYLDKIPDLMD 360
           FFM GLDKRLRPR+KV+EVLKVK LI +NRKIA LL+ GER F+KNYVLK+LD++PDLMD
Sbjct: 301 FFMSGLDKRLRPRFKVVEVLKVKKLI-ENRKIARLLVLGERIFVKNYVLKHLDEVPDLMD 360

Query: 361 IYRGKAAA 369
           IYRG AAA
Sbjct: 361 IYRGNAAA 367

BLAST of MS004318 vs. NCBI nr
Match: XP_022983916.1 (transcription termination factor MTERF9, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 559.7 bits (1441), Expect = 1.9e-155
Identity = 287/368 (77.99%), Postives = 324/368 (88.04%), Query Frame = 0

Query: 1   MAKNSSRILLCFVQKRFISKASGSTLPSSSLYTIEFLKNSCGLASESALSAHRKLQLDEK 60
           M+K SS  LL F+Q RFI+  S STLP +S+ TIEFLKNSCGLA++S  SA RKLQ DEK
Sbjct: 1   MSKISSTFLLYFIQNRFINTVSSSTLPLASVSTIEFLKNSCGLAADSPFSAGRKLQFDEK 60

Query: 61  NSRKYESVLNLLKSYGFEGSQIAKLIARRPSILQSRVDNNLKPKFEFLEEIGFVGPLLPK 120
           +++KYE+VL  LKSYGFE SQIAKLIA++PSILQSRV NNLKPKFEFL++IGFVGPLLPK
Sbjct: 61  SAKKYEAVLGFLKSYGFENSQIAKLIAKQPSILQSRVYNNLKPKFEFLKDIGFVGPLLPK 120

Query: 121 VIISNPIILLRGLDSQLKPSVCFIKEMLESNEKVIAAIRRSSWVLTFDFKGIMQSNIDVL 180
           VI+SNP ILLRGLDS LKPS CFIKEMLES+E+   AIRRSSW+LT  FKG+MQSN++VL
Sbjct: 121 VILSNPGILLRGLDSHLKPSFCFIKEMLESDEQANVAIRRSSWLLTSSFKGVMQSNVNVL 180

Query: 181 LREGVPSRNISKLIVVQPRTIMQKVDRMIFAVNKAKELGIDPTTLMFVQAVRVMVSMNDS 240
           L EGVPSRNISKLI +QPRTIMQKVDRM+ AV K KELGI+P T MFV A+RV+VS++D+
Sbjct: 181 LGEGVPSRNISKLIGLQPRTIMQKVDRMVCAVKKVKELGIEPHTRMFVHALRVVVSISDT 240

Query: 241 TWKKKINVLKSLGWSEKEILTAFKRDPFCLACSEEKMRGVADFCFNTAKLDPETVISYPK 300
           TWKKKINVLKSLGWSEKEILTAFKRDP+CLACSEEKMR VADFC NTAKLDPETVISYPK
Sbjct: 241 TWKKKINVLKSLGWSEKEILTAFKRDPYCLACSEEKMRAVADFCLNTAKLDPETVISYPK 300

Query: 301 FFMYGLDKRLRPRYKVLEVLKVKNLIKKNRKIAWLLIQGERSFLKNYVLKYLDKIPDLMD 360
           FFM GLDK LRPR+KVLEVLKVK LI KNRKIAWLL  GER+F+ NYVLK+LD++PDLMD
Sbjct: 301 FFMSGLDKCLRPRFKVLEVLKVKKLI-KNRKIAWLLFLGERNFVTNYVLKHLDEVPDLMD 360

Query: 361 IYRGKAAA 369
           IYRG AAA
Sbjct: 361 IYRGNAAA 367

BLAST of MS004318 vs. NCBI nr
Match: XP_022983900.1 (transcription termination factor MTERF9, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 559.7 bits (1441), Expect = 1.9e-155
Identity = 285/368 (77.45%), Postives = 322/368 (87.50%), Query Frame = 0

Query: 1   MAKNSSRILLCFVQKRFISKASGSTLPSSSLYTIEFLKNSCGLASESALSAHRKLQLDEK 60
           M+K SS+ LL F+Q RF++  S STLPS+S+ TIEFLKN+CGLA++S  SA RKLQ DEK
Sbjct: 1   MSKISSKFLLYFIQNRFVNTVSSSTLPSASVSTIEFLKNTCGLAADSPSSAGRKLQFDEK 60

Query: 61  NSRKYESVLNLLKSYGFEGSQIAKLIARRPSILQSRVDNNLKPKFEFLEEIGFVGPLLPK 120
           +++KYE+VL  LKSYGFE SQIAKLIA++PSILQSRV NNLKPKFEFLE+IGFVGPLL K
Sbjct: 61  SAKKYEAVLGFLKSYGFENSQIAKLIAKQPSILQSRVYNNLKPKFEFLEDIGFVGPLLAK 120

Query: 121 VIISNPIILLRGLDSQLKPSVCFIKEMLESNEKVIAAIRRSSWVLTFDFKGIMQSNIDVL 180
           VI+ NP ILLRGLDS LKPS CFIKEMLES+E+   AIRRSSW+LT + KG+MQSN++VL
Sbjct: 121 VILLNPSILLRGLDSHLKPSFCFIKEMLESDEQANVAIRRSSWLLTSNLKGVMQSNVNVL 180

Query: 181 LREGVPSRNISKLIVVQPRTIMQKVDRMIFAVNKAKELGIDPTTLMFVQAVRVMVSMNDS 240
           L EGVPSRNISKLI  QPRTIMQKVDRM+ AV K KELG++P T MFV+A+RV++SMND+
Sbjct: 181 LGEGVPSRNISKLIGQQPRTIMQKVDRMVCAVKKVKELGVEPQTRMFVRALRVVLSMNDT 240

Query: 241 TWKKKINVLKSLGWSEKEILTAFKRDPFCLACSEEKMRGVADFCFNTAKLDPETVISYPK 300
           TWKKKINVLKSLGWSEKEILTAFKRDP CLACSEEKMR  ADFC NTAKLDPETVISYPK
Sbjct: 241 TWKKKINVLKSLGWSEKEILTAFKRDPLCLACSEEKMRAAADFCLNTAKLDPETVISYPK 300

Query: 301 FFMYGLDKRLRPRYKVLEVLKVKNLIKKNRKIAWLLIQGERSFLKNYVLKYLDKIPDLMD 360
           FFMYGLDK LRPR+KVLEVLKVK LI KNRKIAWLL  GER F+ NYVLKYLD++PDLMD
Sbjct: 301 FFMYGLDKWLRPRFKVLEVLKVKKLI-KNRKIAWLLFLGEREFVTNYVLKYLDEVPDLMD 360

Query: 361 IYRGKAAA 369
           IYRG AAA
Sbjct: 361 IYRGNAAA 367

BLAST of MS004318 vs. NCBI nr
Match: KAG6601090.1 (Transcription termination factor MTERF9, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 559.3 bits (1440), Expect = 2.5e-155
Identity = 286/368 (77.72%), Postives = 323/368 (87.77%), Query Frame = 0

Query: 1   MAKNSSRILLCFVQKRFISKASGSTLPSSSLYTIEFLKNSCGLASESALSAHRKLQLDEK 60
           M+K SS  LL F+Q RFI+  SGSTLP +S+ TIEFL+NSCGLA++S  SA RKLQ DEK
Sbjct: 1   MSKISSTFLLYFIQNRFINTVSGSTLPLASVSTIEFLENSCGLATDSPSSALRKLQFDEK 60

Query: 61  NSRKYESVLNLLKSYGFEGSQIAKLIARRPSILQSRVDNNLKPKFEFLEEIGFVGPLLPK 120
           ++RKYE+VL  LKSYGFE SQI KLIA+RPSILQSRV +NLKPKFEFLEEIGF GPLLPK
Sbjct: 61  SARKYEAVLGFLKSYGFENSQIVKLIAKRPSILQSRVSSNLKPKFEFLEEIGFFGPLLPK 120

Query: 121 VIISNPIILLRGLDSQLKPSVCFIKEMLESNEKVIAAIRRSSWVLTFDFKGIMQSNIDVL 180
           VI+SNP IL RGLDSQLKPS CFIKEMLES+E+   AIRRSSW+LTFDFKG+MQSN++VL
Sbjct: 121 VILSNPAILQRGLDSQLKPSFCFIKEMLESDEQANFAIRRSSWLLTFDFKGVMQSNVNVL 180

Query: 181 LREGVPSRNISKLIVVQPRTIMQKVDRMIFAVNKAKELGIDPTTLMFVQAVRVMVSMNDS 240
           L EGVPSRNISKLI  QPRTIMQKVDRM+ AV K KELGI+P T MFV+A+RV++SMND+
Sbjct: 181 LGEGVPSRNISKLIAQQPRTIMQKVDRMVCAVKKVKELGIEPQTRMFVRALRVVLSMNDT 240

Query: 241 TWKKKINVLKSLGWSEKEILTAFKRDPFCLACSEEKMRGVADFCFNTAKLDPETVISYPK 300
           TWKKKINV KSLGWSEKEILTAFKRDP+CLACSEEKMR VADFCFN AKLDPETVIS+P 
Sbjct: 241 TWKKKINVFKSLGWSEKEILTAFKRDPYCLACSEEKMRAVADFCFNAAKLDPETVISHPT 300

Query: 301 FFMYGLDKRLRPRYKVLEVLKVKNLIKKNRKIAWLLIQGERSFLKNYVLKYLDKIPDLMD 360
           FFM GLDKRLRPR+KV+EVLKVK LI +NRKIA LL+ GER F+KNYVLK+LD++PDLMD
Sbjct: 301 FFMSGLDKRLRPRFKVVEVLKVKKLI-ENRKIARLLVVGERIFVKNYVLKHLDEVPDLMD 360

Query: 361 IYRGKAAA 369
           IYRG A A
Sbjct: 361 IYRGNATA 367

BLAST of MS004318 vs. NCBI nr
Match: XP_022957146.1 (transcription termination factor MTERF5, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 555.1 bits (1429), Expect = 4.7e-154
Identity = 285/368 (77.45%), Postives = 323/368 (87.77%), Query Frame = 0

Query: 1   MAKNSSRILLCFVQKRFISKASGSTLPSSSLYTIEFLKNSCGLASESALSAHRKLQLDEK 60
           M+K SS  LL F+Q RFI+  SGSTLP +S+ TIEFLKNSCGLA++S  SA RKLQ DEK
Sbjct: 1   MSKISSTFLLYFIQNRFINTVSGSTLPLASVSTIEFLKNSCGLATDSPSSALRKLQFDEK 60

Query: 61  NSRKYESVLNLLKSYGFEGSQIAKLIARRPSILQSRVDNNLKPKFEFLEEIGFVGPLLPK 120
           +++KYE+VL  LKSYGFE SQIAKLIA+R SILQSRV +NLKPKFEFLEEIGFVGPLLPK
Sbjct: 61  SAKKYEAVLGFLKSYGFENSQIAKLIAKRSSILQSRVSSNLKPKFEFLEEIGFVGPLLPK 120

Query: 121 VIISNPIILLRGLDSQLKPSVCFIKEMLESNEKVIAAIRRSSWVLTFDFKGIMQSNIDVL 180
           VI+SNP IL RGLDS LKPS CFIKEMLES+E+   AIRRSSW+LT +FKG+MQSN++VL
Sbjct: 121 VILSNPAILQRGLDSHLKPSFCFIKEMLESDEQANFAIRRSSWLLTSNFKGVMQSNVNVL 180

Query: 181 LREGVPSRNISKLIVVQPRTIMQKVDRMIFAVNKAKELGIDPTTLMFVQAVRVMVSMNDS 240
           L EGVPSRNISKLI  QPRTIMQKVDRM+ AV K KELGI+P T MFV+A+RV++SMND+
Sbjct: 181 LGEGVPSRNISKLIAQQPRTIMQKVDRMVCAVKKVKELGIEPQTRMFVRALRVVLSMNDT 240

Query: 241 TWKKKINVLKSLGWSEKEILTAFKRDPFCLACSEEKMRGVADFCFNTAKLDPETVISYPK 300
           TWKKKINVLKSLGWSEKEILTAFKRDP+CLACSEEKMR VADFCFN AKLDPETVISYP 
Sbjct: 241 TWKKKINVLKSLGWSEKEILTAFKRDPYCLACSEEKMRAVADFCFNAAKLDPETVISYPT 300

Query: 301 FFMYGLDKRLRPRYKVLEVLKVKNLIKKNRKIAWLLIQGERSFLKNYVLKYLDKIPDLMD 360
           FFM GLDKRLRPR+KV+EVLKVK LI +NRKIA LL+ GER F+KNYVLK+LD++PDLM+
Sbjct: 301 FFMSGLDKRLRPRFKVVEVLKVKKLI-ENRKIARLLVVGERIFVKNYVLKHLDEVPDLME 360

Query: 361 IYRGKAAA 369
           IYRG A A
Sbjct: 361 IYRGNATA 367

BLAST of MS004318 vs. ExPASy Swiss-Prot
Match: Q9FM80 (Transcription termination factor MTERF9, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=MTERF9 PE=2 SV=1)

HSP 1 Score: 72.8 bits (177), Expect = 9.2e-12
Identity = 58/261 (22.22%), Postives = 115/261 (44.06%), Query Frame = 0

Query: 69  LNLLKSYGFEGSQIAKLIARRPSILQSRVDNNLKPKFEFLEEIGFVGPLLPKVIISNPII 128
           L+ L S G +   I +++ R+P ILQ  V+NNLK    FL  +G     + +++ + P +
Sbjct: 223 LDYLLSVGVKHRDIKRMLLRQPQILQYTVENNLKAHISFLMGLGIPNSKIGQIVAATPSL 282

Query: 129 LLRGLDSQLKPSVCFIKEMLESNEKVIAAIRRSSWVLTFDFKGIMQSNIDVLLREGVPSR 188
               +++ L+P++ ++ E +                                   G+   
Sbjct: 283 FSYSVENSLRPTIRYLIEEV-----------------------------------GIKET 342

Query: 189 NISKLIVVQPRTIMQKVD-----RMIFAVNKAKELGI--DPTTLMFVQAVRVMVSMNDST 248
           ++ K++ + P+ ++Q++D     R +F    +KELG   D    M  +  +++    D  
Sbjct: 343 DVGKVVQLSPQILVQRLDITWNTRYMFL---SKELGAPRDSVVKMVKKHPQLLHYSIDDG 402

Query: 249 WKKKINVLKSLGWSEKEILTAFKRDPFCLACS-EEKMRGVADFCFNTAKLDPETVISYPK 308
           +  +IN L+S+G    +IL         L+ S E+ ++    +  N    +   +  YP 
Sbjct: 403 FLPRINFLRSIGMCNSDILKVLTSLTQVLSLSLEDNLKPKYMYLVNELNNEVHILTKYPM 445

Query: 309 FFMYGLDKRLRPRYKVLEVLK 322
           +    LD+R+RPR++ L  LK
Sbjct: 463 YLSLSLDQRIRPRHRFLVELK 445

BLAST of MS004318 vs. ExPASy Swiss-Prot
Match: F4IHL3 (Transcription termination factor MTERF2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=MTERF2 PE=3 SV=1)

HSP 1 Score: 67.4 bits (163), Expect = 3.9e-10
Identity = 57/279 (20.43%), Postives = 119/279 (42.65%), Query Frame = 0

Query: 66  ESVLNLLKSYGFEGSQIAKLIARRPSILQSRVDNNLKPKFEFLEEIGFVGPLLPKVIISN 125
           E  +N LK +G    ++ +L+A +P ++   ++   KP  ++   +G     + ++++  
Sbjct: 353 EKKINYLKEFGLSTEEVGRLLAYKPHLMGCSIEERWKPLVKYFYYLGIPKEGMKRILVVK 412

Query: 126 PIILLRGLDSQLKPSVCFIKEMLESNEKVIAAIRRSSWVLTFD-FKGIMQSNIDVLLREG 185
           PI+    L+  + P V F++EM   NE +   + +   +LT   +K I    I +L R G
Sbjct: 413 PILYCIDLEKTIAPKVRFLQEMGIPNEAIGNMLVKFPSLLTNSLYKKIRPVVIFLLTRAG 472

Query: 186 VPSRNISKLIVVQPRTIMQKVDRMIFAVNKAKELGIDPTTLMFVQAVRVMVSMNDSTWKK 245
           V  ++I K+I + P  +   +              ++P    ++                
Sbjct: 473 VTQKDIGKVIAMDPALLGCSIGTK-----------LEPNMRYYI---------------- 532

Query: 246 KINVLKSLGWSEKEILTAFKRDPFCLACSEEKMRGVADFCFNTAKLDPETVISYPKFFMY 305
                 SLG    ++       P  L  + + +R    +   T     + +I +P+FF Y
Sbjct: 533 ------SLGIRFYQLGEMIADFPMLLRYNVDNLRPKYRYLRRTMIRPLQDLIEFPRFFSY 592

Query: 306 GLDKRLRPRYKVLEVLKVKNLIKKNRKIAWLLIQGERSF 344
            L++R+ PR+ ++    V+N +  N K+ ++L   +  F
Sbjct: 593 SLERRIIPRHTIM----VENRV--NFKLRYMLACTDEEF 592

BLAST of MS004318 vs. ExPASy Swiss-Prot
Match: B6TGN4 (Transcription termination factor MTERF4, chloroplastic OS=Zea mays OX=4577 GN=MTERF4 PE=2 SV=1)

HSP 1 Score: 67.4 bits (163), Expect = 3.9e-10
Identity = 59/279 (21.15%), Postives = 123/279 (44.09%), Query Frame = 0

Query: 68  VLNLLKSYGFEGSQIAKLIARRPSILQSRVDNNLKPKFEFLEEIGFVGPLLPKVIISNPI 127
           V+  L+      + + +++ R P +L  +++  +     +L  IG     +  VI   P 
Sbjct: 174 VVKYLQGMDVRPTDVPRVLERYPELLGFKLEGTMSTSVAYLVGIGVGRRQVGSVITRFPE 233

Query: 128 ILLRGLDSQLKPSVCFIKEMLESNEKVIAAIRRSSWVLTFDFKGIMQSNIDVLLREGVPS 187
           +L   +   +KP V  ++ +      +   I +  +VL F  +  ++ NI+ L+  GV  
Sbjct: 234 VLGMRVGKIIKPFVEHLEGIGLQRLAIARIIEKKPYVLGFGLQEKVKPNIEALVDIGVRK 293

Query: 188 RNISKLIVVQPRTIMQKVDRMIFAVNKAKELGIDPTTLMFVQAVRVM---VSMNDSTWKK 247
             ++ +++  P  +  ++   + A     E  I  +   F + +  M   +S+  +   K
Sbjct: 294 EALASIVMQYPDVLGLELRDKLVAQQSLFESSILVSREDFGRVLERMPQAISLGRAAVLK 353

Query: 248 KINVLKSLGWSEKEILTAFKRDPFCLACSEEKMRGVADFCFNTAKLDPETVISYPKFFMY 307
            +N L + G+   ++       P  LA + + MR   ++  N  + D E ++ +P FF Y
Sbjct: 354 HVNFLTACGFMLSQVSKMVVACPQLLALNIDIMRMNFEYFKNEMERDLEELVEFPAFFTY 413

Query: 308 GLDKRLRPRYKVLEVLKVKNLIKKNRKIAWLLIQGERSF 344
           G++  +RPR+   E++  K L      +AWLL   +  F
Sbjct: 414 GIESTVRPRH---EMVSRKGL---TCSLAWLLNCSDAKF 446

BLAST of MS004318 vs. ExPASy Swiss-Prot
Match: Q6AUK6 (Transcription termination factor MTERF4, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0404000 PE=2 SV=1)

HSP 1 Score: 67.4 bits (163), Expect = 3.9e-10
Identity = 59/279 (21.15%), Postives = 120/279 (43.01%), Query Frame = 0

Query: 68  VLNLLKSYGFEGSQIAKLIARRPSILQSRVDNNLKPKFEFLEEIGFVGPLLPKVIISNPI 127
           V+  L+        + +++ R P +L  +++  +     +L  IG     +  VI   P 
Sbjct: 183 VVKYLQGMDVRPHDVPRVLERYPELLGFKLEGTMSTSIAYLVGIGVARRQVGSVITRFPE 242

Query: 128 ILLRGLDSQLKPSVCFIKEMLESNEKVIAAIRRSSWVLTFDFKGIMQSNIDVLLREGVPS 187
           +L   +   +KP V  ++ +      +   I +  +VL F  +  ++ NI+ LL  GV  
Sbjct: 243 VLGMRVGKIIKPFVEHLEGIGLQRLAIARIIEKKPYVLGFGLEDKVKPNIEALLEFGVRK 302

Query: 188 RNISKLIVVQPRTIMQKVDRMIFAVNKAKELGIDPTTLMFVQAVRVM---VSMNDSTWKK 247
             ++ ++   P  +  ++   +       E  I  ++  F + +  M   +S+  +   K
Sbjct: 303 EALAFIVAQYPDILGIELRDKLATQQSLFESSILVSSEDFGRVIERMPQAISLGRTAVLK 362

Query: 248 KINVLKSLGWSEKEILTAFKRDPFCLACSEEKMRGVADFCFNTAKLDPETVISYPKFFMY 307
            +N L S G+   ++       P  LA + + M+   ++  N  + D E ++ +P FF Y
Sbjct: 363 HVNFLTSCGFLLSQVSKMVVACPQLLALNMDIMKMSFEYFQNEMERDLEELVEFPAFFTY 422

Query: 308 GLDKRLRPRYKVLEVLKVKNLIKKNRKIAWLLIQGERSF 344
           GL+  +RPR+   E++  K        +AWLL   +  F
Sbjct: 423 GLESTVRPRH---EMVAKKGF---TCSLAWLLNCSDAKF 455

BLAST of MS004318 vs. ExPASy Swiss-Prot
Match: Q9ZT96 (Transcription termination factor MTERF4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=MTERF4 PE=1 SV=1)

HSP 1 Score: 62.0 bits (149), Expect = 1.6e-08
Identity = 57/281 (20.28%), Postives = 119/281 (42.35%), Query Frame = 0

Query: 68  VLNLLKSYGFEGSQIAKLIARRPSILQSRVDNNLKPKFEFLEEIGFVGPLLPKVIISNPI 127
           V+  L+    + S + +++ R P +L  +++  +     +L  IG     +  ++   P 
Sbjct: 218 VVKYLQGLDIKPSDVPRVLERYPEVLGFKLEGTMSTSVAYLVGIGVARREIGGILTRYPE 277

Query: 128 ILLRGLDSQLKPSVCFIKEMLESNEKVIAAIRRSSWVLTFDFKGIMQSNIDVLLREGVPS 187
           IL   +   +KP V +++ +          I +   +L F+    ++ N+ +L    V  
Sbjct: 278 ILGMRVARIIKPLVEYLEVLGIPRLAAARLIEKRPHILGFELDDTVKPNVQILQDFNVRE 337

Query: 188 RNISKLIVVQPRTIMQKVDRMIFAVNK--AKELGIDPTTL-MFVQAVRVMVSMNDSTWKK 247
            ++  +I   P  I   +   +    K     + ++P  L   ++ +   VS+++S   K
Sbjct: 338 TSLPSIIAQYPEIIGIDLKPKLDTQRKLLCSAIHLNPEDLGSLIERMPQFVSLSESPMLK 397

Query: 248 KINVLKSLGWSEKEILTAFKRDPFCLACSEEKMRGVADFCFNTAKLDPETVISYPKFFMY 307
            I+ L   G+S  +        P  LA +   M+   ++     K   + ++ +P FF Y
Sbjct: 398 HIDFLTKCGFSIDQTREMVIGCPQVLALNLGIMKLSFEYFQKEMKRPLQDLVDFPAFFTY 457

Query: 308 GLDKRLRPRYKVLEVLKVKNLIKKNRK--IAWLLIQGERSF 344
           GL+  ++PR+        K +IKK  K  +AW+L   +  F
Sbjct: 458 GLESTVKPRH--------KKIIKKGIKCSLAWMLNCSDEKF 490

BLAST of MS004318 vs. ExPASy TrEMBL
Match: A0A6J1J3P9 (transcription termination factor MTERF9, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111482390 PE=3 SV=1)

HSP 1 Score: 559.7 bits (1441), Expect = 9.2e-156
Identity = 287/368 (77.99%), Postives = 324/368 (88.04%), Query Frame = 0

Query: 1   MAKNSSRILLCFVQKRFISKASGSTLPSSSLYTIEFLKNSCGLASESALSAHRKLQLDEK 60
           M+K SS  LL F+Q RFI+  S STLP +S+ TIEFLKNSCGLA++S  SA RKLQ DEK
Sbjct: 1   MSKISSTFLLYFIQNRFINTVSSSTLPLASVSTIEFLKNSCGLAADSPFSAGRKLQFDEK 60

Query: 61  NSRKYESVLNLLKSYGFEGSQIAKLIARRPSILQSRVDNNLKPKFEFLEEIGFVGPLLPK 120
           +++KYE+VL  LKSYGFE SQIAKLIA++PSILQSRV NNLKPKFEFL++IGFVGPLLPK
Sbjct: 61  SAKKYEAVLGFLKSYGFENSQIAKLIAKQPSILQSRVYNNLKPKFEFLKDIGFVGPLLPK 120

Query: 121 VIISNPIILLRGLDSQLKPSVCFIKEMLESNEKVIAAIRRSSWVLTFDFKGIMQSNIDVL 180
           VI+SNP ILLRGLDS LKPS CFIKEMLES+E+   AIRRSSW+LT  FKG+MQSN++VL
Sbjct: 121 VILSNPGILLRGLDSHLKPSFCFIKEMLESDEQANVAIRRSSWLLTSSFKGVMQSNVNVL 180

Query: 181 LREGVPSRNISKLIVVQPRTIMQKVDRMIFAVNKAKELGIDPTTLMFVQAVRVMVSMNDS 240
           L EGVPSRNISKLI +QPRTIMQKVDRM+ AV K KELGI+P T MFV A+RV+VS++D+
Sbjct: 181 LGEGVPSRNISKLIGLQPRTIMQKVDRMVCAVKKVKELGIEPHTRMFVHALRVVVSISDT 240

Query: 241 TWKKKINVLKSLGWSEKEILTAFKRDPFCLACSEEKMRGVADFCFNTAKLDPETVISYPK 300
           TWKKKINVLKSLGWSEKEILTAFKRDP+CLACSEEKMR VADFC NTAKLDPETVISYPK
Sbjct: 241 TWKKKINVLKSLGWSEKEILTAFKRDPYCLACSEEKMRAVADFCLNTAKLDPETVISYPK 300

Query: 301 FFMYGLDKRLRPRYKVLEVLKVKNLIKKNRKIAWLLIQGERSFLKNYVLKYLDKIPDLMD 360
           FFM GLDK LRPR+KVLEVLKVK LI KNRKIAWLL  GER+F+ NYVLK+LD++PDLMD
Sbjct: 301 FFMSGLDKCLRPRFKVLEVLKVKKLI-KNRKIAWLLFLGERNFVTNYVLKHLDEVPDLMD 360

Query: 361 IYRGKAAA 369
           IYRG AAA
Sbjct: 361 IYRGNAAA 367

BLAST of MS004318 vs. ExPASy TrEMBL
Match: A0A6J1J774 (transcription termination factor MTERF9, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111482382 PE=3 SV=1)

HSP 1 Score: 559.7 bits (1441), Expect = 9.2e-156
Identity = 285/368 (77.45%), Postives = 322/368 (87.50%), Query Frame = 0

Query: 1   MAKNSSRILLCFVQKRFISKASGSTLPSSSLYTIEFLKNSCGLASESALSAHRKLQLDEK 60
           M+K SS+ LL F+Q RF++  S STLPS+S+ TIEFLKN+CGLA++S  SA RKLQ DEK
Sbjct: 1   MSKISSKFLLYFIQNRFVNTVSSSTLPSASVSTIEFLKNTCGLAADSPSSAGRKLQFDEK 60

Query: 61  NSRKYESVLNLLKSYGFEGSQIAKLIARRPSILQSRVDNNLKPKFEFLEEIGFVGPLLPK 120
           +++KYE+VL  LKSYGFE SQIAKLIA++PSILQSRV NNLKPKFEFLE+IGFVGPLL K
Sbjct: 61  SAKKYEAVLGFLKSYGFENSQIAKLIAKQPSILQSRVYNNLKPKFEFLEDIGFVGPLLAK 120

Query: 121 VIISNPIILLRGLDSQLKPSVCFIKEMLESNEKVIAAIRRSSWVLTFDFKGIMQSNIDVL 180
           VI+ NP ILLRGLDS LKPS CFIKEMLES+E+   AIRRSSW+LT + KG+MQSN++VL
Sbjct: 121 VILLNPSILLRGLDSHLKPSFCFIKEMLESDEQANVAIRRSSWLLTSNLKGVMQSNVNVL 180

Query: 181 LREGVPSRNISKLIVVQPRTIMQKVDRMIFAVNKAKELGIDPTTLMFVQAVRVMVSMNDS 240
           L EGVPSRNISKLI  QPRTIMQKVDRM+ AV K KELG++P T MFV+A+RV++SMND+
Sbjct: 181 LGEGVPSRNISKLIGQQPRTIMQKVDRMVCAVKKVKELGVEPQTRMFVRALRVVLSMNDT 240

Query: 241 TWKKKINVLKSLGWSEKEILTAFKRDPFCLACSEEKMRGVADFCFNTAKLDPETVISYPK 300
           TWKKKINVLKSLGWSEKEILTAFKRDP CLACSEEKMR  ADFC NTAKLDPETVISYPK
Sbjct: 241 TWKKKINVLKSLGWSEKEILTAFKRDPLCLACSEEKMRAAADFCLNTAKLDPETVISYPK 300

Query: 301 FFMYGLDKRLRPRYKVLEVLKVKNLIKKNRKIAWLLIQGERSFLKNYVLKYLDKIPDLMD 360
           FFMYGLDK LRPR+KVLEVLKVK LI KNRKIAWLL  GER F+ NYVLKYLD++PDLMD
Sbjct: 301 FFMYGLDKWLRPRFKVLEVLKVKKLI-KNRKIAWLLFLGEREFVTNYVLKYLDEVPDLMD 360

Query: 361 IYRGKAAA 369
           IYRG AAA
Sbjct: 361 IYRGNAAA 367

BLAST of MS004318 vs. ExPASy TrEMBL
Match: A0A6J1GYF2 (transcription termination factor MTERF5, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111458616 PE=3 SV=1)

HSP 1 Score: 555.1 bits (1429), Expect = 2.3e-154
Identity = 285/368 (77.45%), Postives = 323/368 (87.77%), Query Frame = 0

Query: 1   MAKNSSRILLCFVQKRFISKASGSTLPSSSLYTIEFLKNSCGLASESALSAHRKLQLDEK 60
           M+K SS  LL F+Q RFI+  SGSTLP +S+ TIEFLKNSCGLA++S  SA RKLQ DEK
Sbjct: 1   MSKISSTFLLYFIQNRFINTVSGSTLPLASVSTIEFLKNSCGLATDSPSSALRKLQFDEK 60

Query: 61  NSRKYESVLNLLKSYGFEGSQIAKLIARRPSILQSRVDNNLKPKFEFLEEIGFVGPLLPK 120
           +++KYE+VL  LKSYGFE SQIAKLIA+R SILQSRV +NLKPKFEFLEEIGFVGPLLPK
Sbjct: 61  SAKKYEAVLGFLKSYGFENSQIAKLIAKRSSILQSRVSSNLKPKFEFLEEIGFVGPLLPK 120

Query: 121 VIISNPIILLRGLDSQLKPSVCFIKEMLESNEKVIAAIRRSSWVLTFDFKGIMQSNIDVL 180
           VI+SNP IL RGLDS LKPS CFIKEMLES+E+   AIRRSSW+LT +FKG+MQSN++VL
Sbjct: 121 VILSNPAILQRGLDSHLKPSFCFIKEMLESDEQANFAIRRSSWLLTSNFKGVMQSNVNVL 180

Query: 181 LREGVPSRNISKLIVVQPRTIMQKVDRMIFAVNKAKELGIDPTTLMFVQAVRVMVSMNDS 240
           L EGVPSRNISKLI  QPRTIMQKVDRM+ AV K KELGI+P T MFV+A+RV++SMND+
Sbjct: 181 LGEGVPSRNISKLIAQQPRTIMQKVDRMVCAVKKVKELGIEPQTRMFVRALRVVLSMNDT 240

Query: 241 TWKKKINVLKSLGWSEKEILTAFKRDPFCLACSEEKMRGVADFCFNTAKLDPETVISYPK 300
           TWKKKINVLKSLGWSEKEILTAFKRDP+CLACSEEKMR VADFCFN AKLDPETVISYP 
Sbjct: 241 TWKKKINVLKSLGWSEKEILTAFKRDPYCLACSEEKMRAVADFCFNAAKLDPETVISYPT 300

Query: 301 FFMYGLDKRLRPRYKVLEVLKVKNLIKKNRKIAWLLIQGERSFLKNYVLKYLDKIPDLMD 360
           FFM GLDKRLRPR+KV+EVLKVK LI +NRKIA LL+ GER F+KNYVLK+LD++PDLM+
Sbjct: 301 FFMSGLDKRLRPRFKVVEVLKVKKLI-ENRKIARLLVVGERIFVKNYVLKHLDEVPDLME 360

Query: 361 IYRGKAAA 369
           IYRG A A
Sbjct: 361 IYRGNATA 367

BLAST of MS004318 vs. ExPASy TrEMBL
Match: A0A6J1GYC4 (transcription termination factor MTERF5, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111458617 PE=3 SV=1)

HSP 1 Score: 549.7 bits (1415), Expect = 9.5e-153
Identity = 281/368 (76.36%), Postives = 321/368 (87.23%), Query Frame = 0

Query: 1   MAKNSSRILLCFVQKRFISKASGSTLPSSSLYTIEFLKNSCGLASESALSAHRKLQLDEK 60
           M+K SS+ LL F+Q RF++  S STLP +S+ TIEFLKNSCGLA++S  SA RKLQ DEK
Sbjct: 1   MSKISSKFLLYFIQNRFVNTVSSSTLPLASVSTIEFLKNSCGLATDSPSSALRKLQFDEK 60

Query: 61  NSRKYESVLNLLKSYGFEGSQIAKLIARRPSILQSRVDNNLKPKFEFLEEIGFVGPLLPK 120
           +++KYE+V   LKSYGFE SQIAKLIA+RPSILQSRV +NLKPKFEFLEEIGFVGPLLPK
Sbjct: 61  SAKKYEAVPRFLKSYGFENSQIAKLIAKRPSILQSRVSSNLKPKFEFLEEIGFVGPLLPK 120

Query: 121 VIISNPIILLRGLDSQLKPSVCFIKEMLESNEKVIAAIRRSSWVLTFDFKGIMQSNIDVL 180
           VI+SNP IL RGLDS LKPS CFIKEMLES+E+   AIRR SW+LT + KG+MQSN++VL
Sbjct: 121 VILSNPAILQRGLDSHLKPSFCFIKEMLESDEQANFAIRRFSWLLTSNLKGVMQSNVNVL 180

Query: 181 LREGVPSRNISKLIVVQPRTIMQKVDRMIFAVNKAKELGIDPTTLMFVQAVRVMVSMNDS 240
           L EGVPSRNISKLI +QPRTI+QKVDRM+ AV K KELGI+P T MFV A+RV+VS++D+
Sbjct: 181 LSEGVPSRNISKLIRLQPRTILQKVDRMVCAVKKVKELGIEPHTRMFVHALRVVVSISDT 240

Query: 241 TWKKKINVLKSLGWSEKEILTAFKRDPFCLACSEEKMRGVADFCFNTAKLDPETVISYPK 300
           TWKKKINVLKSLGWSEKEILTAFKRDP+CLACSEEKMR VADFC NTAKLDPETVISYPK
Sbjct: 241 TWKKKINVLKSLGWSEKEILTAFKRDPYCLACSEEKMRAVADFCLNTAKLDPETVISYPK 300

Query: 301 FFMYGLDKRLRPRYKVLEVLKVKNLIKKNRKIAWLLIQGERSFLKNYVLKYLDKIPDLMD 360
           FFM GLDK LRPR+KV+EVLKVK LI KN KIAWLL  GER+F+ NYVLK+LD++PDLMD
Sbjct: 301 FFMSGLDKCLRPRFKVVEVLKVKKLI-KNTKIAWLLFLGERNFVTNYVLKHLDEVPDLMD 360

Query: 361 IYRGKAAA 369
           IYRG AAA
Sbjct: 361 IYRGNAAA 367

BLAST of MS004318 vs. ExPASy TrEMBL
Match: A0A6J1GZE2 (transcription termination factor MTERF5, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111458618 PE=3 SV=1)

HSP 1 Score: 541.2 bits (1393), Expect = 3.4e-150
Identity = 278/368 (75.54%), Postives = 319/368 (86.68%), Query Frame = 0

Query: 1   MAKNSSRILLCFVQKRFISKASGSTLPSSSLYTIEFLKNSCGLASESALSAHRKLQLDEK 60
           M+K SS+ LL F+Q RF++ AS STLPS+S+ TIEFLKNSCGLA  S  SA RKLQ DEK
Sbjct: 1   MSKISSKFLLYFIQNRFVNTASSSTLPSASVSTIEFLKNSCGLAPGSPSSAGRKLQFDEK 60

Query: 61  NSRKYESVLNLLKSYGFEGSQIAKLIARRPSILQSRVDNNLKPKFEFLEEIGFVGPLLPK 120
           +++KYE+VL  LKSYGFE SQIAKLIA++PSILQSRV NNLKPKFEFLE+IGFVGPLLPK
Sbjct: 61  SAKKYEAVLGFLKSYGFENSQIAKLIAKQPSILQSRVCNNLKPKFEFLEDIGFVGPLLPK 120

Query: 121 VIISNPIILLRGLDSQLKPSVCFIKEMLESNEKVIAAIRRSSWVLTFDFKGIMQSNIDVL 180
           VI+ +P IL RGLDS LKPS CFIKEML S+E+   AI RSSW+LT + KG+MQSN++VL
Sbjct: 121 VILLDPSILQRGLDSHLKPSFCFIKEMLGSDEQANVAILRSSWLLTSNLKGVMQSNVNVL 180

Query: 181 LREGVPSRNISKLIVVQPRTIMQKVDRMIFAVNKAKELGIDPTTLMFVQAVRVMVSMNDS 240
           L EGVPSRNISKLI  QPRTIM KVDR++ AV K KELGI+P T MFV+A+RV++SMND+
Sbjct: 181 LGEGVPSRNISKLIGQQPRTIMHKVDRVVCAVKKIKELGIEPQTCMFVRALRVVMSMNDT 240

Query: 241 TWKKKINVLKSLGWSEKEILTAFKRDPFCLACSEEKMRGVADFCFNTAKLDPETVISYPK 300
           TWKKKINVLKSLGWSEKEILTAFK+DP+CLACSEEKMR VADFC NTAKLDPETVIS PK
Sbjct: 241 TWKKKINVLKSLGWSEKEILTAFKKDPYCLACSEEKMRAVADFCLNTAKLDPETVISNPK 300

Query: 301 FFMYGLDKRLRPRYKVLEVLKVKNLIKKNRKIAWLLIQGERSFLKNYVLKYLDKIPDLMD 360
           FFMYGLDK LRPR+KV+EVLKVK LI KN KIAWLL+  ER F+KNYVLK+LD++PDLMD
Sbjct: 301 FFMYGLDKWLRPRFKVVEVLKVKKLI-KNTKIAWLLLLDERIFVKNYVLKHLDEVPDLMD 360

Query: 361 IYRGKAAA 369
           I+RG AAA
Sbjct: 361 IFRGNAAA 367

BLAST of MS004318 vs. TAIR 10
Match: AT5G07900.1 (Mitochondrial transcription termination factor family protein )

HSP 1 Score: 221.5 bits (563), Expect = 1.1e-57
Identity = 120/334 (35.93%), Postives = 193/334 (57.78%), Query Frame = 0

Query: 32  YTIEFLKNSCGLASESALSAHRKLQLDEKNSRKYESVLNLLKSYGFEGSQIAKLIARRPS 91
           +T+ +L +SCGL+ +SA  A RKL LD  +  +  +VLNLL+ +GF  +QI+ L+ +RP 
Sbjct: 70  FTLNYLIDSCGLSPDSATVASRKLLLD--SPERPNTVLNLLRDHGFTTAQISSLVKKRPV 129

Query: 92  ILQSRVDNNLKPKFEFLEEIGFVGPLLPKVIISNPIILLRGLDSQLKPSVCFIKEMLESN 151
           +L +  ++ L PK  F   IG    LL + + S+P IL R L +QL PS  F+K +L+S+
Sbjct: 130 LLLANAESVLLPKLSFFLSIGVSKSLLARTLASDPTILTRSLVNQLIPSYNFLKSVLDSD 189

Query: 152 EKVIAAIRRSSWVLTFDFKGIMQSNIDVLLREGVPSRNISKLIVVQPRTIMQKVDRMIFA 211
           EK++AA+RR++WV   D    +  NI+ +   GVP + I  L+   P  +MQK       
Sbjct: 190 EKIVAALRRTTWVFLEDHTKNLVPNINYMAETGVPEKCIKLLLTHFPEAVMQKNHEFQAI 249

Query: 212 VNKAKELGIDPTTLMFVQAVRVMVSM-NDSTWKKKINVLKSLGWSEKEILTAFKRDPFCL 271
             +A+E+G +P    FV A+  +    N S W K   V +  GWSE +I+ AFK+ P C+
Sbjct: 250 AKQAQEMGFNPQKSTFVLAIHALSGKGNKSIWDKCFEVYQRWGWSEDDIMCAFKKHPHCM 309

Query: 272 ACSEEKMRGVADFCFNTAKLDPETVISYPKFFMYGLDKRLRPRYKVLEVLKVKNLIKKNR 331
             SE K+    ++  N   + P ++   P    + L+KR+ PR  V +VL    L+K++ 
Sbjct: 310 MLSERKINRTMEYFVNEMNMAPRSIAQCPVVLFFSLEKRIIPRCSVTKVLVSNGLVKEDW 369

Query: 332 KIAWLLIQGERSFLKNYVLKYLDKIPDLMDIYRG 365
            +  LL+  E+ FL+  V+KY +++P+LM++Y G
Sbjct: 370 SLTSLLVPVEKVFLEKLVIKYEEELPELMNLYLG 401

BLAST of MS004318 vs. TAIR 10
Match: AT1G21150.1 (Mitochondrial transcription termination factor family protein )

HSP 1 Score: 156.8 bits (395), Expect = 3.4e-38
Identity = 95/334 (28.44%), Postives = 167/334 (50.00%), Query Frame = 0

Query: 27  PSSSLYTIEFLKNSCGLASESALSAHRKLQLDEKNSRKYESVLNLLKSYGFEGSQIAKLI 86
           P    +T+ +L +SCGL+ ESA S  R ++L   +S+K +SVL L K +GF   QI  +I
Sbjct: 52  PCLESFTVSYLVDSCGLSLESAKSNSRFVKL--VSSKKPDSVLALFKDHGFTNDQITSVI 111

Query: 87  ARRPSILQSRVDNNLKPKFEFLEEIGFVGPLLPKVIISNPIILLRGLDSQLKPSVCFIKE 146
              P +L    ++ + PK  F   IGF      K+I S+P +L   L  +L P    +K 
Sbjct: 112 KSFPRVLSLSPEDVISPKLMFFSSIGFSTSDTAKMISSSPKMLSYSLHKRLIPCYDSLKS 171

Query: 147 MLESNEKVIAAIRRSSWVLTFDFKGIMQSNIDVLLREGVPSRNISKLIVVQPRTIMQKVD 206
           +L   E V+  ++R     +      +   + +    GVP ++I  L+   P T   +  
Sbjct: 172 ILVEEESVVKCLKRGIRCFSLKITHCVSLRVSICRELGVPDKSIKWLVQASPFTFFSRER 231

Query: 207 RMIFAVNKAKELGIDPTTLMFVQAVRVMVSMNDSTWKKKINVLKSLGWSEKEILTAFKRD 266
           R    +N+    G DP    FV A+      ++S  ++K  + +  GWS+++ + A  R 
Sbjct: 232 RFNEVLNRVCSYGFDPKKAGFVHAMVAFDCTSESAMERKFKLFQCFGWSKEDFVAAIMRF 291

Query: 267 PFCLACSEEKMRGVADFCFNTAKLDPETVISYPKFFMYGLDKRLRPRYKVLEVLKVKNLI 326
           P C+  S+EK+    ++  N   L    +++ P      ++KR++PR +V+ +L  K L+
Sbjct: 292 PNCVTVSDEKIMYTLEYLVNNIGLQARDIVARPVVLSLSMEKRIKPRNQVISLLLSKGLV 351

Query: 327 KKNRKIAWLLIQGERS-FLKNYVLKYLDKIPDLM 360
           KK     + +++ + S F+  +VLKY D++P L+
Sbjct: 352 KKEDINYFTILKLKSSEFMDKFVLKYQDEMPQLV 383

BLAST of MS004318 vs. TAIR 10
Match: AT1G61970.1 (Mitochondrial transcription termination factor family protein )

HSP 1 Score: 142.1 bits (357), Expect = 8.8e-34
Identity = 114/394 (28.93%), Postives = 195/394 (49.49%), Query Frame = 0

Query: 17  FISKASGSTLP-----SSSLYTIEFLKNSCGLASESALSAHRKLQLDEKNSRKYESVLNL 76
           F S AS + L        + +T+ +L +S GL ++ A S  RK+  ++KN+   +SVLNL
Sbjct: 33  FFSSASAACLSPRVGRKGNNFTVSYLVDSLGLTTKLAESISRKVSFEDKNNP--DSVLNL 92

Query: 77  LKSYGFEGSQIAKLIARRPSILQSRVDNNLKPKFEFLEEIGFVGPLLPKVIISNPIILLR 136
           L S+GF GSQI+ +I   P +L +  + +L PK +FL+  G     + +++ S P IL +
Sbjct: 93  LTSHGFTGSQISTIIRDYPQLLIADAEKSLGPKLQFLQSRGASSSEITEIVSSVPEILGK 152

Query: 137 GLDSQLKPSVCFIKEML----ESNEKVIAAIRRSSWVLTFDFKGIMQSNIDVLLREGVPS 196
                +     FIK+ L      NEK+  ++ + +             N+ VL   G+P 
Sbjct: 153 KGHKTISVYYDFIKDTLLEKSSKNEKLCHSLPQGNLENKI-------RNVSVLRELGMPH 212

Query: 197 RNISKLIVVQPRTIMQKVDRMIFAVNKAKELGIDPTTLMFVQAVRVMVSMNDSTWKKKIN 256
           + +  L++   + +  K ++    + K  E+G DPTT  FV+A++V+  MN+ T ++K++
Sbjct: 213 KLLFSLLISDSQPVCGK-EKFEETLKKVVEMGFDPTTSKFVEALQVIYKMNEKTIEEKVH 272

Query: 257 VLKSLGW------------------SEKEILTA--------FKRDPF---------CLAC 316
           + KSLG+                  SEK++L +        F RD F         C+  
Sbjct: 273 LYKSLGFDVGDVWSSFKKWPISLRVSEKKMLDSIETFLGLGFSRDEFAKMVKHFPPCIGL 332

Query: 317 SEEKMRGVADFCFNTAKLDPETVISYPKFFMYGLDKRLRPRYKVLEVLKVKNLIKKN-RK 364
           S E ++   +F         + V+S P  F Y L+KR+ PR  V++ L  K L++     
Sbjct: 333 STETVKKKTEFLVKKMNWPLKAVVSNPAVFGYSLEKRIVPRGNVIKALMSKGLMRNELPS 392

BLAST of MS004318 vs. TAIR 10
Match: AT1G61970.2 (Mitochondrial transcription termination factor family protein )

HSP 1 Score: 142.1 bits (357), Expect = 8.8e-34
Identity = 114/394 (28.93%), Postives = 195/394 (49.49%), Query Frame = 0

Query: 17  FISKASGSTLP-----SSSLYTIEFLKNSCGLASESALSAHRKLQLDEKNSRKYESVLNL 76
           F S AS + L        + +T+ +L +S GL ++ A S  RK+  ++KN+   +SVLNL
Sbjct: 33  FFSSASAACLSPRVGRKGNNFTVSYLVDSLGLTTKLAESISRKVSFEDKNNP--DSVLNL 92

Query: 77  LKSYGFEGSQIAKLIARRPSILQSRVDNNLKPKFEFLEEIGFVGPLLPKVIISNPIILLR 136
           L S+GF GSQI+ +I   P +L +  + +L PK +FL+  G     + +++ S P IL +
Sbjct: 93  LTSHGFTGSQISTIIRDYPQLLIADAEKSLGPKLQFLQSRGASSSEITEIVSSVPEILGK 152

Query: 137 GLDSQLKPSVCFIKEML----ESNEKVIAAIRRSSWVLTFDFKGIMQSNIDVLLREGVPS 196
                +     FIK+ L      NEK+  ++ + +             N+ VL   G+P 
Sbjct: 153 KGHKTISVYYDFIKDTLLEKSSKNEKLCHSLPQGNLENKI-------RNVSVLRELGMPH 212

Query: 197 RNISKLIVVQPRTIMQKVDRMIFAVNKAKELGIDPTTLMFVQAVRVMVSMNDSTWKKKIN 256
           + +  L++   + +  K ++    + K  E+G DPTT  FV+A++V+  MN+ T ++K++
Sbjct: 213 KLLFSLLISDSQPVCGK-EKFEETLKKVVEMGFDPTTSKFVEALQVIYKMNEKTIEEKVH 272

Query: 257 VLKSLGW------------------SEKEILTA--------FKRDPF---------CLAC 316
           + KSLG+                  SEK++L +        F RD F         C+  
Sbjct: 273 LYKSLGFDVGDVWSSFKKWPISLRVSEKKMLDSIETFLGLGFSRDEFAKMVKHFPPCIGL 332

Query: 317 SEEKMRGVADFCFNTAKLDPETVISYPKFFMYGLDKRLRPRYKVLEVLKVKNLIKKN-RK 364
           S E ++   +F         + V+S P  F Y L+KR+ PR  V++ L  K L++     
Sbjct: 333 STETVKKKTEFLVKKMNWPLKAVVSNPAVFGYSLEKRIVPRGNVIKALMSKGLMRNELPS 392

BLAST of MS004318 vs. TAIR 10
Match: AT5G64950.1 (Mitochondrial transcription termination factor family protein )

HSP 1 Score: 138.3 bits (347), Expect = 1.3e-32
Identity = 98/347 (28.24%), Postives = 182/347 (52.45%), Query Frame = 0

Query: 17  FISKASGSTLPSSSLYTIEFLKNSCGLASESALSAHRKLQLDEKNSRKYESVLNLLKSYG 76
           F S A+ +++ + +   +EFL+++ G     A++   +   + K+  +  SV+ +LKSY 
Sbjct: 27  FSSNATPASISNPAF--VEFLRDN-GFQKPLAIAIAMRYP-NLKSLEQPRSVIQMLKSYS 86

Query: 77  FEGSQIAKLIARRPSILQSRVDNNLKPKFEFLEEIGFVGPLLPKVIISNPIILLRGLDSQ 136
           F  +QI K I   P ++   V+  L+PK  F ++IGF G  L K +  N  ++   L  +
Sbjct: 87  FSDTQIQKSIRVHPRMMFYNVEKILEPKLRFFKDIGFTGSGLGKFVSQNSSVVGVSLVKK 146

Query: 137 LKPSVCFIKEML-ESNEKVIAAIRRSSW-VLTFDFKGIMQSNIDVLLREGVPSRNISKLI 196
           L P+V  +K ++   +E +   + R  W +L+ D    +  NI  L   G+    ++ L+
Sbjct: 147 LIPTVEILKSIVAPKHEDLPVILSRCGWLLLSRDPNLFLLPNISYLETCGIVGSQLASLL 206

Query: 197 VVQPRTIMQKVDRMIFAVNKAKELGIDPTTLMFVQAVRVMVSMNDSTWKKKINVLKSLGW 256
             QPR      +++   V++A +LG    + M V AV  + S+++ T+ +K+ +  + G+
Sbjct: 207 RRQPRIFNLSEEKLRGYVSRALDLGFTLNSRMLVHAVISLSSLSEKTFDRKVKLFMANGF 266

Query: 257 SEKEILTAFKRDPFCLACSEEKMRGVADFCFNTAKLDPETVISYPKFFMYGLDKRLRPRY 316
           SE EI    +R P  + CSE+K+    +F      L+ E +   P    Y L+KR+ PR 
Sbjct: 267 SEDEITDIIRRSPGLIRCSEDKLTLGFEFYLKRMGLEREALAKRPCVLSYNLEKRVIPRL 326

Query: 317 KVLEVLKVKNLI----KKNRKIAWLLIQGERSFLKNYVLKYLDKIPD 358
           KVL++L+ K L+    KK + +  ++   E +FL+ YV+++ D+I +
Sbjct: 327 KVLQILREKGLLLKEEKKKKNMVGIVEMTEEAFLEKYVVRFGDEIAE 369

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_023529167.12.9e-15678.53transcription termination factor MTERF5, chloroplastic-like [Cucurbita pepo subs... [more]
XP_022983916.11.9e-15577.99transcription termination factor MTERF9, chloroplastic-like [Cucurbita maxima][more]
XP_022983900.11.9e-15577.45transcription termination factor MTERF9, chloroplastic-like [Cucurbita maxima][more]
KAG6601090.12.5e-15577.72Transcription termination factor MTERF9, chloroplastic, partial [Cucurbita argyr... [more]
XP_022957146.14.7e-15477.45transcription termination factor MTERF5, chloroplastic-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q9FM809.2e-1222.22Transcription termination factor MTERF9, chloroplastic OS=Arabidopsis thaliana O... [more]
F4IHL33.9e-1020.43Transcription termination factor MTERF2, chloroplastic OS=Arabidopsis thaliana O... [more]
B6TGN43.9e-1021.15Transcription termination factor MTERF4, chloroplastic OS=Zea mays OX=4577 GN=MT... [more]
Q6AUK63.9e-1021.15Transcription termination factor MTERF4, chloroplastic OS=Oryza sativa subsp. ja... [more]
Q9ZT961.6e-0820.28Transcription termination factor MTERF4, chloroplastic OS=Arabidopsis thaliana O... [more]
Match NameE-valueIdentityDescription
A0A6J1J3P99.2e-15677.99transcription termination factor MTERF9, chloroplastic-like OS=Cucurbita maxima ... [more]
A0A6J1J7749.2e-15677.45transcription termination factor MTERF9, chloroplastic-like OS=Cucurbita maxima ... [more]
A0A6J1GYF22.3e-15477.45transcription termination factor MTERF5, chloroplastic-like OS=Cucurbita moschat... [more]
A0A6J1GYC49.5e-15376.36transcription termination factor MTERF5, chloroplastic-like OS=Cucurbita moschat... [more]
A0A6J1GZE23.4e-15075.54transcription termination factor MTERF5, chloroplastic-like OS=Cucurbita moschat... [more]
Match NameE-valueIdentityDescription
AT5G07900.11.1e-5735.93Mitochondrial transcription termination factor family protein [more]
AT1G21150.13.4e-3828.44Mitochondrial transcription termination factor family protein [more]
AT1G61970.18.8e-3428.93Mitochondrial transcription termination factor family protein [more]
AT1G61970.28.8e-3428.93Mitochondrial transcription termination factor family protein [more]
AT5G64950.11.3e-3228.24Mitochondrial transcription termination factor family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003690Transcription termination factor, mitochondrial/chloroplasticSMARTSM00733mt_12coord: 292..323
e-value: 0.053
score: 22.6
coord: 83..114
e-value: 2.1E-6
score: 37.3
coord: 119..150
e-value: 14.0
score: 14.6
coord: 260..291
e-value: 1100.0
score: 3.7
coord: 225..255
e-value: 16.0
score: 14.4
coord: 155..186
e-value: 250.0
score: 8.5
coord: 191..221
e-value: 620.0
score: 5.5
IPR003690Transcription termination factor, mitochondrial/chloroplasticPFAMPF02536mTERFcoord: 69..276
e-value: 1.6E-27
score: 96.2
coord: 228..343
e-value: 1.5E-18
score: 66.8
IPR003690Transcription termination factor, mitochondrial/chloroplasticPANTHERPTHR13068CGI-12 PROTEIN-RELATEDcoord: 19..366
IPR038538MTERF superfamily, mitochondrial/chloroplasticGENE3D1.25.70.10Transcription termination factor 3, mitochondrialcoord: 136..354
e-value: 6.7E-40
score: 138.9
NoneNo IPR availablePANTHERPTHR13068:SF31TRANSCRIPTION TERMINATION FACTOR MTERF15, MITOCHONDRIAL-LIKEcoord: 19..366

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS004318.1MS004318.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006355 regulation of transcription, DNA-templated
molecular_function GO:0003690 double-stranded DNA binding