MS004312 (gene) Bitter gourd (TR) v1

Overview


Name	MS004312
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	KHG/KDPG aldolase-like
Location	scaffold92: 1754263 .. 1757312 (-)
RNA-Seq Expression	MS004312
Synteny	MS004312

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTTAGTTTCTAGGAGAATCAACATGTGGTCTTTGCCTGCTATAGGGTACTGTAACCCTTTTGCATGTGCTCGCTTTAGAGTTTTCTGCGCTTCTAGTCAGCTCTCTGTATCTCCCAAAGACAAGACCCTGAAAAAAATTTACAATTCTGGAGTCATCGCTTGCCTTCGTGCTAGCAGGTAACTCATTCTCATGTAACACTTTCTGCATTTTAGAATGTAGTGGTGCTGGATTCTCTTAGAACTCTATGCTTGACATGATCGAACTATTAGTTCTTAGTGGCCAGCTGGTTGCCCCAAGGTCTTCATTACTTTCTCCTTATTGTTTACAACTTTACTGGTTAGCTGATAGTTGACAGCAAGGTCTGTTATCTATATAAACCCATTTACATGGTTATATTACAAAAAATGGTCAGATAGCACCAACCTAATACTAAAATACATTTTCATTGTTTCATTAGATGTTGAATAGGTGGTTCGCAAAAGTAGAACCTCGCCTCTTTTCCGGAATTCATTGCTTATGTCTTCTTTAAAAAGTAAGAAAACATATTAGACTTGAAATATAAATAGTCTAGACAAAGGTAAAGAAAGATAAAGGTAGACCACAGAAAGTGTTGGTGACATGGATCCAAAGAAAGGCATCAAAGTGAGCCACACTCCCTGCCTCCTTCAAAAGCTGCTTGAACACACCTTCACGTTTTTTTCTAATCCAAAATATCTGGGCTTCCGAAGTTTACTAGAGTTAAGAAATTTAATATCGTTTGTCTAGAGGTATCTAGAGTCTTGGTCTGGACTTTAATTATTTTCTTAATTCACTATAGTGCAGAGCTGGCAATGAGTGCTGCTTGTGCTGCGCTAAATGGTGGAGTATCAGTCGTGAGTGCTCTTAATTCTAAGTTGTCATTGTTCCCGTATATTTGAACTAGTTTGAATAATATTCAGTGATACATCCAAATATTCTATTGGTATATGGTTTCTTATTGCTGACGCTTTCTAAATGATTAGTAGAACTAAAAAAAGATTGAAGAAATTGTCCTCATGACGCTTTTGTAAATGGTAAGTGCATGATGAATAATAATAACCACAGAAAGCGGCTAGAGCATGTCAACTTTATTGAAATATATTTTTTTCTTTTTTGATGTATTGATTGATGGTTTCTAACCTTTTTTTTTTTTTTCATGTCAATATGCAGCTGGAGATTGTTACGTCAACACCAGGTGTTTTTGAGGTTGTTGTTGAGAATATTTTCCTTTTCATTGTACTTGTTTCTAATTTTAAGAATTTATCTTATTTTTCTTTCATTTTTTTCATCTGTTATAAAAATTGAAACAGGTTCTACAGCAGTTGCTGCAAGACTATCCTACCAAAACAGTGGGAGTAATTATTTTCTTTGCTTTTTATGGGCGTGATGCTTTCTTAATGTTTGTAATATATCCTTGCTACACCTAGGTCAAAATTTTTGTAGCCTTTGGTTTTGACTTTTGAGCCTTTGATGGATTAGGATGAACTATAGCTTTACTCTTAATGCTTACAAAAATATAACTTGTCAATCTCACAAAAGATGGATGATGTTGAGTTCTTATGAAGATACGCAAATGTTCTGCAGGTTGGGACAGTTCTTAATATTAAGGACGCAAAGGATGCTGTAAAAGGTGGAGCCAAGTTTCTAATGAGTCCTACTCTGGTGAAGGTAAGCATTGATAGATGACGAGCATACCACATTTACTTTTTTGCTTATATATCACCGTGGACTTTTTTAATTGTATGCTGATTCCTCTTTATCCTATTTTCATGTTTTCCTTTTTTTTTCCCGTCATTGTTTGTCGTGATTTTTCATCCTGGAGTCCAACTAAAAGAGACTACGAGGATATTTTCTTCTCTCAGATTATTTTCACTGGTTAACAGGGTATAATGGATGATATTGAAGATGAATTCTTGTATATACCTGGTGTGATGACCCCAACAGAAGTAATCACATAACATTTATTTGCCTCTTTTCCCTATAAATTCTCAAGGATTCATCCTTTTTACTTAATGAAAATATGCTTTACAGATACTGACCGCATACGAAGCTGGTGCTGAGATTGTTAAAGTAAGTAAATTTGTTGTTTGGTTTAGCAAGCTCGTTGGTAACAATAAGCAGTTGTCATTTCCCATCACTGCATTCACCACTTATTTCGCTTAGATAGATGACCAGTATACCTCGTTTACATTACAACTTTCAAGGAACTACCTCATTTTCCTTGGAAAACTAGAAATATATACATAATTCTTAGGGTTATGTGTTTGTCTCAAACAAACATGATAAAATTTATATTTCTTAATTAAAAAGAAACGTAAATATACTACTCAAAACTAATTTTCATTTATTTTGTTCCCTTGATGCTTTTCAATACAGTAAGTCCTAATGGAAATTTCTTAAATGGTAATTATTATGCAATTACGAGTAATAAAGAAGGAAAAACCGATGCAAGTTATTTCTTTTCCCATTTGTTCTTCAGTGCATAAAATTTTTCGTCTGCTTGTGCTTGCGTTCCATTTTGACATCAGAATATGGTTCTAGCCTTGTTATTATTATTTTTCTATGGATTTATTTTGATTTTCTAATCTTGAAAAATAGGTTTATCCAGTTTCCGCATTAGGTGGTATTAAATATATATCGGCACTCAAGAAGCCATTTCCCCATATCTCAATGGTTGCTTCTCAAGGCATAACTATTGGTTGGTACTCAACTAAAAGTTAAAACTATTTGCCCCTTGCGTTCTTTTGTTTTGTTTTTAATCATGCGGTTCACTGTTTCTGTTTTCTGGGCCAGTCAGTTTTTTTGGAAATTATTTTCATCAGTTTGATATAACTTGATCTGCTTCTGAGTTCATTTGTTACTTCTAAATCTGTGTAGACTCAATAGGGGATTATATCAGACAAGGAGCATCTTCGGTTGTTTTATCTGATGCAATATTTAACAAGGAGTTTATGGACCAACGAAATTTTGATGGAATATTTCGACTTTCTAAGCTGGCTGCTTCTCAGGCGATGGAAGCTTTAGAATGG

mRNA sequence

ATGTTAGTTTCTAGGAGAATCAACATGTGGTCTTTGCCTGCTATAGGGTACTGTAACCCTTTTGCATGTGCTCGCTTTAGAGTTTTCTGCGCTTCTAGTCAGCTCTCTGTATCTCCCAAAGACAAGACCCTGAAAAAAATTTACAATTCTGGAGTCATCGCTTGCCTTCGTGCTAGCAGTGCAGAGCTGGCAATGAGTGCTGCTTGTGCTGCGCTAAATGGTGGAGTATCAGTCCTGGAGATTGTTACGTCAACACCAGGTGTTTTTGAGGTTGTTCTACAGCAGTTGCTGCAAGACTATCCTACCAAAACAGTGGGAGTTGGGACAGTTCTTAATATTAAGGACGCAAAGGATGCTGTAAAAGGTGGAGCCAAGTTTCTAATGAGTCCTACTCTGGTGAAGGGTATAATGGATGATATTGAAGATGAATTCTTGTATATACCTGGTGTGATGACCCCAACAGAAATACTGACCGCATACGAAGCTGGTGCTGAGATTGTTAAAGTTTATCCAGTTTCCGCATTAGGTGGTATTAAATATATATCGGCACTCAAGAAGCCATTTCCCCATATCTCAATGGTTGCTTCTCAAGGCATAACTATTGACTCAATAGGGGATTATATCAGACAAGGAGCATCTTCGGTTGTTTTATCTGATGCAATATTTAACAAGGAGTTTATGGACCAACGAAATTTTGATGGAATATTTCGACTTTCTAAGCTGGCTGCTTCTCAGGCGATGGAAGCTTTAGAATGG

Coding sequence (CDS)

ATGTTAGTTTCTAGGAGAATCAACATGTGGTCTTTGCCTGCTATAGGGTACTGTAACCCTTTTGCATGTGCTCGCTTTAGAGTTTTCTGCGCTTCTAGTCAGCTCTCTGTATCTCCCAAAGACAAGACCCTGAAAAAAATTTACAATTCTGGAGTCATCGCTTGCCTTCGTGCTAGCAGTGCAGAGCTGGCAATGAGTGCTGCTTGTGCTGCGCTAAATGGTGGAGTATCAGTCCTGGAGATTGTTACGTCAACACCAGGTGTTTTTGAGGTTGTTCTACAGCAGTTGCTGCAAGACTATCCTACCAAAACAGTGGGAGTTGGGACAGTTCTTAATATTAAGGACGCAAAGGATGCTGTAAAAGGTGGAGCCAAGTTTCTAATGAGTCCTACTCTGGTGAAGGGTATAATGGATGATATTGAAGATGAATTCTTGTATATACCTGGTGTGATGACCCCAACAGAAATACTGACCGCATACGAAGCTGGTGCTGAGATTGTTAAAGTTTATCCAGTTTCCGCATTAGGTGGTATTAAATATATATCGGCACTCAAGAAGCCATTTCCCCATATCTCAATGGTTGCTTCTCAAGGCATAACTATTGACTCAATAGGGGATTATATCAGACAAGGAGCATCTTCGGTTGTTTTATCTGATGCAATATTTAACAAGGAGTTTATGGACCAACGAAATTTTGATGGAATATTTCGACTTTCTAAGCTGGCTGCTTCTCAGGCGATGGAAGCTTTAGAATGG

Protein sequence

MLVSRRINMWSLPAIGYCNPFACARFRVFCASSQLSVSPKDKTLKKIYNSGVIACLRASSAELAMSAACAALNGGVSVLEIVTSTPGVFEVVLQQLLQDYPTKTVGVGTVLNIKDAKDAVKGGAKFLMSPTLVKGIMDDIEDEFLYIPGVMTPTEILTAYEAGAEIVKVYPVSALGGIKYISALKKPFPHISMVASQGITIDSIGDYIRQGASSVVLSDAIFNKEFMDQRNFDGIFRLSKLAASQAMEALEW

Homology

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BLAST of MS004312 vs. NCBI nr
Match: XP_022139077.1 (KHG/KDPG aldolase isoform X3 [Momordica charantia] >XP_022139078.1 KHG/KDPG aldolase isoform X3 [Momordica charantia])

HSP 1 Score: 478.0 bits (1229), Expect = 5.0e-131
Identity = 248/252 (98.41%), Postives = 249/252 (98.81%), Query Frame = 0


Query: 1   MLVSRRINMWSLPAIGYCNPFACARFRVFCASSQLSVSPKDKTLKKIYNSGVIACLRASS 60
           MLVSRRINMWSLPAIGYCNPFACARF VFCAS QLSVSPKDKTLKKIYNSGVIACLRASS
Sbjct: 1   MLVSRRINMWSLPAIGYCNPFACARFTVFCASRQLSVSPKDKTLKKIYNSGVIACLRASS 60

Query: 61  AELAMSAACAALNGGVSVLEIVTSTPGVFEVVLQQLLQDYPTKTVGVGTVLNIKDAKDAV 120
           AELAMSAACAALNGGVSVLEIVTSTPGVFE VLQQLLQDYPTKTVGVGTVLNIKDAKDAV
Sbjct: 61  AELAMSAACAALNGGVSVLEIVTSTPGVFE-VLQQLLQDYPTKTVGVGTVLNIKDAKDAV 120

Query: 121 KGGAKFLMSPTLVKGIMDDIEDEFLYIPGVMTPTEILTAYEAGAEIVKVYPVSALGGIKY 180
           KGGAKFLMSPT+VKGIMDDIEDEFLYIPGVMTPTEILTAYEAGAEIVKVYPVSALGGIKY
Sbjct: 121 KGGAKFLMSPTMVKGIMDDIEDEFLYIPGVMTPTEILTAYEAGAEIVKVYPVSALGGIKY 180

Query: 181 ISALKKPFPHISMVASQGITIDSIGDYIRQGASSVVLSDAIFNKEFMDQRNFDGIFRLSK 240
           ISALKKPFPHISMVASQGITIDSIGDYIRQGASSVVLSDAIFNKEFMDQRNFDGIFRLSK
Sbjct: 181 ISALKKPFPHISMVASQGITIDSIGDYIRQGASSVVLSDAIFNKEFMDQRNFDGIFRLSK 240

Query: 241 LAASQAMEALEW 253
           LAASQAMEALEW
Sbjct: 241 LAASQAMEALEW 251

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BLAST of MS004312 vs. NCBI nr
Match: XP_022139079.1 (KHG/KDPG aldolase isoform X4 [Momordica charantia])

HSP 1 Score: 443.0 bits (1138), Expect = 1.8e-120
Identity = 233/252 (92.46%), Postives = 234/252 (92.86%), Query Frame = 0


Query: 1   MLVSRRINMWSLPAIGYCNPFACARFRVFCASSQLSVSPKDKTLKKIYNSGVIACLRASS 60
           MLVSRRINMWSLPAIGYCNPFACARF VFCAS QLSVSPKDKTLKKIYNSGVIACLRASS
Sbjct: 1   MLVSRRINMWSLPAIGYCNPFACARFTVFCASRQLSVSPKDKTLKKIYNSGVIACLRASS 60

Query: 61  AELAMSAACAALNGGVSVLEIVTSTPGVFEVVLQQLLQDYPTKTVGVGTVLNIKDAKDAV 120
           AELAMSAACAALNGGVSVLEIVTSTPGVFE                VGTVLNIKDAKDAV
Sbjct: 61  AELAMSAACAALNGGVSVLEIVTSTPGVFE----------------VGTVLNIKDAKDAV 120

Query: 121 KGGAKFLMSPTLVKGIMDDIEDEFLYIPGVMTPTEILTAYEAGAEIVKVYPVSALGGIKY 180
           KGGAKFLMSPT+VKGIMDDIEDEFLYIPGVMTPTEILTAYEAGAEIVKVYPVSALGGIKY
Sbjct: 121 KGGAKFLMSPTMVKGIMDDIEDEFLYIPGVMTPTEILTAYEAGAEIVKVYPVSALGGIKY 180

Query: 181 ISALKKPFPHISMVASQGITIDSIGDYIRQGASSVVLSDAIFNKEFMDQRNFDGIFRLSK 240
           ISALKKPFPHISMVASQGITIDSIGDYIRQGASSVVLSDAIFNKEFMDQRNFDGIFRLSK
Sbjct: 181 ISALKKPFPHISMVASQGITIDSIGDYIRQGASSVVLSDAIFNKEFMDQRNFDGIFRLSK 236

Query: 241 LAASQAMEALEW 253
           LAASQAMEALEW
Sbjct: 241 LAASQAMEALEW 236

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BLAST of MS004312 vs. NCBI nr
Match: XP_038892213.1 (KHG/KDPG aldolase isoform X2 [Benincasa hispida] >XP_038892214.1 KHG/KDPG aldolase isoform X2 [Benincasa hispida] >XP_038892215.1 KHG/KDPG aldolase isoform X2 [Benincasa hispida])

HSP 1 Score: 431.4 bits (1108), Expect = 5.3e-117
Identity = 226/252 (89.68%), Postives = 237/252 (94.05%), Query Frame = 0


Query: 1   MLVSRRINMWSLPAIGYCNPFACARFRVFCASSQLSVSPKDKTLKKIYNSGVIACLRASS 60
           MLVSR I+MWSLPAIG  NPFA ARFRVFCASSQL VSPKDKTL+ I NSGVIACLRASS
Sbjct: 1   MLVSRSISMWSLPAIGCWNPFAFARFRVFCASSQLPVSPKDKTLRTINNSGVIACLRASS 60

Query: 61  AELAMSAACAALNGGVSVLEIVTSTPGVFEVVLQQLLQDYPTKTVGVGTVLNIKDAKDAV 120
           AELAMSAACAALNGG+SVLEIV STPGV E VL QLLQDYPT+T+GVGTVLNIKDAK+AV
Sbjct: 61  AELAMSAACAALNGGISVLEIVMSTPGVLE-VLYQLLQDYPTRTLGVGTVLNIKDAKNAV 120

Query: 121 KGGAKFLMSPTLVKGIMDDIEDEFLYIPGVMTPTEILTAYEAGAEIVKVYPVSALGGIKY 180
           K GAKFLMSPT+VKGIMDDIE EFLYIPGVMTPTE+LTAYEAGAEIVKVYPVSALGGIKY
Sbjct: 121 KAGAKFLMSPTMVKGIMDDIEGEFLYIPGVMTPTEVLTAYEAGAEIVKVYPVSALGGIKY 180

Query: 181 ISALKKPFPHISMVASQGITIDSIGDYIRQGASSVVLSDAIFNKEFMDQRNFDGIFRLSK 240
           ISALKKPFPHISMVASQGITI+S GDYIRQGASSVVLSDAIFNKEFMDQ+NFDGIF+LSK
Sbjct: 181 ISALKKPFPHISMVASQGITIESTGDYIRQGASSVVLSDAIFNKEFMDQKNFDGIFQLSK 240

Query: 241 LAASQAMEALEW 253
           LAAS AMEALEW
Sbjct: 241 LAASVAMEALEW 251

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BLAST of MS004312 vs. NCBI nr
Match: XP_023511849.1 (KHG/KDPG aldolase isoform X4 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 427.2 bits (1097), Expect = 1.0e-115
Identity = 222/252 (88.10%), Postives = 238/252 (94.44%), Query Frame = 0


Query: 1   MLVSRRINMWSLPAIGYCNPFACARFRVFCASSQLSVSPKDKTLKKIYNSGVIACLRASS 60
           MLVSRRI+MWSLPAIG  NPFA ARFRV CASSQL VSPKDKTL+ IYNSGVIACLRASS
Sbjct: 1   MLVSRRISMWSLPAIGCWNPFAFARFRVSCASSQLPVSPKDKTLRTIYNSGVIACLRASS 60

Query: 61  AELAMSAACAALNGGVSVLEIVTSTPGVFEVVLQQLLQDYPTKTVGVGTVLNIKDAKDAV 120
           AELAMSAACAALNGGVSVLEIV STPGV E VLQQLLQDYPT+T+GVGTVLN+KDAK+AV
Sbjct: 61  AELAMSAACAALNGGVSVLEIVMSTPGVLE-VLQQLLQDYPTRTLGVGTVLNVKDAKNAV 120

Query: 121 KGGAKFLMSPTLVKGIMDDIEDEFLYIPGVMTPTEILTAYEAGAEIVKVYPVSALGGIKY 180
           + GAKFLMSPT+VKGIMDD+E EFLYIPGVMTPTE+LTAYEAGA+IVKVYPVSALGGIKY
Sbjct: 121 EAGAKFLMSPTMVKGIMDDLEGEFLYIPGVMTPTEVLTAYEAGAQIVKVYPVSALGGIKY 180

Query: 181 ISALKKPFPHISMVASQGITIDSIGDYIRQGASSVVLSDAIFNKEFMDQRNFDGIFRLSK 240
           ISALKKPFPHISMVASQGITI+S GDYIR+GASSVVLSDAIFNKEFM Q+NFDGI +LSK
Sbjct: 181 ISALKKPFPHISMVASQGITIESTGDYIREGASSVVLSDAIFNKEFMKQKNFDGISQLSK 240

Query: 241 LAASQAMEALEW 253
           LAAS+AMEALEW
Sbjct: 241 LAASRAMEALEW 251

[Close]

BLAST of MS004312 vs. NCBI nr
Match: XP_022957426.1 (KHG/KDPG aldolase isoform X2 [Cucurbita moschata] >XP_022957427.1 KHG/KDPG aldolase isoform X2 [Cucurbita moschata] >XP_022957428.1 KHG/KDPG aldolase isoform X2 [Cucurbita moschata])

HSP 1 Score: 424.5 bits (1090), Expect = 6.5e-115
Identity = 220/252 (87.30%), Postives = 238/252 (94.44%), Query Frame = 0


Query: 1   MLVSRRINMWSLPAIGYCNPFACARFRVFCASSQLSVSPKDKTLKKIYNSGVIACLRASS 60
           MLV+RRI+MWSLPAIG  NPFA ARFRV CASSQL VSPKDKTL+ IYNSGVIACLRASS
Sbjct: 1   MLVARRISMWSLPAIGCWNPFAFARFRVSCASSQLPVSPKDKTLRTIYNSGVIACLRASS 60

Query: 61  AELAMSAACAALNGGVSVLEIVTSTPGVFEVVLQQLLQDYPTKTVGVGTVLNIKDAKDAV 120
           AELAMSAACAALNGGVSVLEIV STPGV E VLQQLLQDYPT+T+GVGTVLN+KDAK+AV
Sbjct: 61  AELAMSAACAALNGGVSVLEIVMSTPGVLE-VLQQLLQDYPTRTLGVGTVLNVKDAKNAV 120

Query: 121 KGGAKFLMSPTLVKGIMDDIEDEFLYIPGVMTPTEILTAYEAGAEIVKVYPVSALGGIKY 180
           + GAKFLMSPT+VKGIMDD+E EFLYIPGVMTPTE+LTAYEAGA+IVKVYPVSALGGIKY
Sbjct: 121 EAGAKFLMSPTMVKGIMDDLEGEFLYIPGVMTPTEVLTAYEAGAQIVKVYPVSALGGIKY 180

Query: 181 ISALKKPFPHISMVASQGITIDSIGDYIRQGASSVVLSDAIFNKEFMDQRNFDGIFRLSK 240
           ISALKKPFPHISMVASQGITI+S GDYIR+GASSVVLSDAIFNKEFM Q+NF+GI +LSK
Sbjct: 181 ISALKKPFPHISMVASQGITIESTGDYIREGASSVVLSDAIFNKEFMKQKNFEGISQLSK 240

Query: 241 LAASQAMEALEW 253
           LAAS+AMEALEW
Sbjct: 241 LAASRAMEALEW 251

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BLAST of MS004312 vs. ExPASy Swiss-Prot
Match: P50846 (KHG/KDPG aldolase OS=Bacillus subtilis (strain 168) OX=224308 GN=kdgA PE=2 SV=1)

HSP 1 Score: 84.7 bits (208), Expect = 1.6e-15
Identity = 49/171 (28.65%), Postives = 97/171 (56.73%), Query Frame = 0


Query: 46  KIYNSGVIACLRASSAELAMSAACAALNGGVSVLEIVTSTPGVFEVVLQQLLQDYPTKTV 105
           ++  + +IA +R+   + A     + L+ G+  +E+  +TPG  +++  +  ++     +
Sbjct: 9   RLKEAKLIAVIRSKDKQEACQQIESLLDKGIRAVEVTYTTPGASDII--ESFRNREDILI 68

Query: 106 GVGTVLNIKDAKDAVKGGAKFLMSPTLVKGIMDDIE-DEFLYIPGVMTPTEILTAYEAGA 165
           G GTV++ + A +A K GA+F++SP     + + +   +  YIPGV+TP+EI+ A   G 
Sbjct: 69  GAGTVISAQQAGEAAKAGAQFIVSPGFSADLAEHLSFVKTHYIPGVLTPSEIMEALTFGF 128

Query: 166 EIVKVYPVSALGGIKYISALKKPFPHISMVASQGITIDSIGDYIRQGASSV 216
             +K++P S + GI ++  L  PFP ++ + + GI    + D++R GA +V
Sbjct: 129 TTLKLFP-SGVFGIPFMKNLAGPFPQVTFIPTGGIHPSEVPDWLRAGAGAV 176

[Close]

BLAST of MS004312 vs. ExPASy Swiss-Prot
Match: Q15X88 (2-dehydro-3-deoxy-phosphogluconate aldolase OS=Pseudoalteromonas atlantica (strain T6c / ATCC BAA-1087) OX=342610 GN=Patl_0974 PE=1 SV=1)

HSP 1 Score: 74.7 bits (182), Expect = 1.7e-12
Identity = 56/208 (26.92%), Postives = 99/208 (47.60%), Query Frame = 0


Query: 47  IYNSG-VIACLRASSAELAMSAACAALNGGVSVLEIVTSTPGVFEVVLQQLLQDYPTKTV 106
           +++ G V+  L     + A+  A A + GG+ VLE+   T    +V+ + +  + P   +
Sbjct: 12  VFSQGPVVPVLVIKDVKHAVPLAKALIAGGIRVLEVTLRTEAALDVI-KAIATEVPDAII 71

Query: 107 GVGTVLNIKDAKDAVKGGAKFLMSPTLVKGIMD-DIEDEFLYIPGVMTPTEILTAYEAGA 166
           G GTV N K   +    GA F +SP +   ++D   +     IPG+ + +E++   + G 
Sbjct: 72  GAGTVTNAKQLAEVEAAGAMFAISPGMTSDLLDAGNKGGIALIPGISSISELMRGIDFGY 131

Query: 167 EIVKVYPVSALGGIKYISALKKPFPHISMVASQGITIDSIGDYIR------QGASSVVLS 226
              K +P  A GG+K I A+  PFP I+   + GI+  +  +Y+        G S +   
Sbjct: 132 THFKFFPAEASGGVKAIKAIGGPFPDIAFCPTGGISPTNYLEYLSLPNVRCAGGSWLAPD 191

Query: 227 DAIFNKEFMDQRNFDGIFRLSKLAASQA 247
           DA+      +  ++D I  L+K A + A
Sbjct: 192 DAV------EAGDWDRITELAKQAVAGA 212

[Close]

BLAST of MS004312 vs. ExPASy Swiss-Prot
Match: O68283 (2-dehydro-3-deoxy-phosphogluconate aldolase OS=Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) OX=208964 GN=eda PE=3 SV=2)

HSP 1 Score: 73.6 bits (179), Expect = 3.7e-12
Identity = 53/187 (28.34%), Postives = 94/187 (50.27%), Query Frame = 0


Query: 63  LAMSAACAALNGGVSVLEIVTSTPGVFEVVLQQLLQDYPTKTVGVGTVLNIKDAKDAVKG 122
           L M+ A AA  GG++ LEI   T       +++L ++ P   +G GTVL+ +    A K 
Sbjct: 33  LPMADALAA--GGLTALEITLRTAHGL-TAIRRLSEERPHLRIGAGTVLDPRTFAAAEKA 92

Query: 123 GAKFLMSPTLVKGIMD-DIEDEFLYIPGVMTPTEILTAYEAGAEIVKVYPVSALGGIKYI 182
           GA F+++P     ++   ++ E   +PGV + +EI+ AY  G    K++P    GG   +
Sbjct: 93  GASFVVTPGCTDELLRFALDSEVPLLPGVASASEIMLAYRHGYRRFKLFPAEVSGGPAAL 152

Query: 183 SALKKPFPHISMVASQGITIDSIGDYIRQGASSVVLSDAIFNKEFMDQRNFDGIFRLSKL 242
            A   PFP I    + G++++++ DY+       V    +  K  +D+ ++  + RLS+ 
Sbjct: 153 KAFSGPFPDIRFCPTGGVSLNNLADYLAVPNVMCVGGTWMLPKAVVDRGDWAQVERLSRE 212

Query: 243 AASQAME 249
           A  +  E
Sbjct: 213 ALERFAE 216

[Close]

BLAST of MS004312 vs. ExPASy Swiss-Prot
Match: O83578 (Putative KHG/KDPG aldolase OS=Treponema pallidum (strain Nichols) OX=243276 GN=eda PE=3 SV=1)

HSP 1 Score: 72.0 bits (175), Expect = 1.1e-11
Identity = 47/158 (29.75%), Postives = 81/158 (51.27%), Query Frame = 0


Query: 52  VIACLRASSAELAMSAACAALNGGVSVLEIVTSTPGVFEVVLQQLLQDYPTKTVGVGTVL 111
           VI  +     E A+  A A + GG+  +E+   T    E +   + Q+     +G GTVL
Sbjct: 13  VIPVVTLERVEDAVPLARALITGGIRCMEVTFRTLVAAEAI-AAIRQECADVLLGAGTVL 72

Query: 112 NIKDAKDAVKGGAKFLMSPTL-VKGIMDDIEDEFLYIPGVMTPTEILTAYEAGAEIVKVY 171
            ++ A+ A   GA+F++SP    + +   +      IPG+ + TEI  A E G  +VK +
Sbjct: 73  TVEQAQQAQAAGAQFVVSPGFNPRVVAHCLGHGVPIIPGIASATEIERALEFGISVVKFF 132

Query: 172 PVSALGGIKYISALKKPFPHISMVASQGITIDSIGDYI 209
           P   LGG   +SAL +P+  +  V + GI ++++ +Y+
Sbjct: 133 PAELLGGTAMMSALARPYTAVRFVPTGGIHLNNLAEYV 169

[Close]

BLAST of MS004312 vs. ExPASy Swiss-Prot
Match: Q6BF16 (2-dehydro-3-deoxy-6-phosphogalactonate aldolase OS=Escherichia coli (strain K12) OX=83333 GN=dgoA PE=1 SV=1)

HSP 1 Score: 64.7 bits (156), Expect = 1.7e-09
Identity = 49/174 (28.16%), Postives = 90/174 (51.72%), Query Frame = 0


Query: 52  VIACLRASSAELAMSAACAALNGGVSVLEIVTSTPGVFEVVLQQLLQDYPTKT-VGVGTV 111
           +IA LR  + + A++   A ++ G   +EI  ++P  +E  +  ++  Y  K  +G GTV
Sbjct: 9   LIAILRGITPDEALAHVGAVIDAGFDAVEIPLNSP-QWEQSIPAIVDAYGDKALIGAGTV 68

Query: 112 LNIKDAKDAVKGGAKFLMSPTLVKGIMDDIEDEFLYI-PGVMTPTEILTAYEAGAEIVKV 171
           L  +      + G + +++P +   ++       + + PG  T TE  TA EAGA+ +K+
Sbjct: 69  LKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGMTVCPGCATATEAFTALEAGAQALKI 128

Query: 172 YPVSALGGIKYISALKKPFP-HISMVASQGITIDSIGDYIRQGASSVVLSDAIF 223
           +P SA G  +YI ALK   P  I++ A  G+T +++  +I  G +   L   ++
Sbjct: 129 FPSSAFGP-QYIKALKAVLPSDIAVFAVGGVTPENLAQWIDAGCAGAGLGSDLY 180

[Close]

BLAST of MS004312 vs. ExPASy TrEMBL
Match: A0A6J1CEU6 (KHG/KDPG aldolase isoform X3 OS=Momordica charantia OX=3673 GN=LOC111010082 PE=4 SV=1)

HSP 1 Score: 478.0 bits (1229), Expect = 2.4e-131
Identity = 248/252 (98.41%), Postives = 249/252 (98.81%), Query Frame = 0


Query: 1   MLVSRRINMWSLPAIGYCNPFACARFRVFCASSQLSVSPKDKTLKKIYNSGVIACLRASS 60
           MLVSRRINMWSLPAIGYCNPFACARF VFCAS QLSVSPKDKTLKKIYNSGVIACLRASS
Sbjct: 1   MLVSRRINMWSLPAIGYCNPFACARFTVFCASRQLSVSPKDKTLKKIYNSGVIACLRASS 60

Query: 61  AELAMSAACAALNGGVSVLEIVTSTPGVFEVVLQQLLQDYPTKTVGVGTVLNIKDAKDAV 120
           AELAMSAACAALNGGVSVLEIVTSTPGVFE VLQQLLQDYPTKTVGVGTVLNIKDAKDAV
Sbjct: 61  AELAMSAACAALNGGVSVLEIVTSTPGVFE-VLQQLLQDYPTKTVGVGTVLNIKDAKDAV 120

Query: 121 KGGAKFLMSPTLVKGIMDDIEDEFLYIPGVMTPTEILTAYEAGAEIVKVYPVSALGGIKY 180
           KGGAKFLMSPT+VKGIMDDIEDEFLYIPGVMTPTEILTAYEAGAEIVKVYPVSALGGIKY
Sbjct: 121 KGGAKFLMSPTMVKGIMDDIEDEFLYIPGVMTPTEILTAYEAGAEIVKVYPVSALGGIKY 180

Query: 181 ISALKKPFPHISMVASQGITIDSIGDYIRQGASSVVLSDAIFNKEFMDQRNFDGIFRLSK 240
           ISALKKPFPHISMVASQGITIDSIGDYIRQGASSVVLSDAIFNKEFMDQRNFDGIFRLSK
Sbjct: 181 ISALKKPFPHISMVASQGITIDSIGDYIRQGASSVVLSDAIFNKEFMDQRNFDGIFRLSK 240

Query: 241 LAASQAMEALEW 253
           LAASQAMEALEW
Sbjct: 241 LAASQAMEALEW 251

[Close]

BLAST of MS004312 vs. ExPASy TrEMBL
Match: A0A6J1CBW7 (KHG/KDPG aldolase isoform X4 OS=Momordica charantia OX=3673 GN=LOC111010082 PE=4 SV=1)

HSP 1 Score: 443.0 bits (1138), Expect = 8.6e-121
Identity = 233/252 (92.46%), Postives = 234/252 (92.86%), Query Frame = 0


Query: 1   MLVSRRINMWSLPAIGYCNPFACARFRVFCASSQLSVSPKDKTLKKIYNSGVIACLRASS 60
           MLVSRRINMWSLPAIGYCNPFACARF VFCAS QLSVSPKDKTLKKIYNSGVIACLRASS
Sbjct: 1   MLVSRRINMWSLPAIGYCNPFACARFTVFCASRQLSVSPKDKTLKKIYNSGVIACLRASS 60

Query: 61  AELAMSAACAALNGGVSVLEIVTSTPGVFEVVLQQLLQDYPTKTVGVGTVLNIKDAKDAV 120
           AELAMSAACAALNGGVSVLEIVTSTPGVFE                VGTVLNIKDAKDAV
Sbjct: 61  AELAMSAACAALNGGVSVLEIVTSTPGVFE----------------VGTVLNIKDAKDAV 120

Query: 121 KGGAKFLMSPTLVKGIMDDIEDEFLYIPGVMTPTEILTAYEAGAEIVKVYPVSALGGIKY 180
           KGGAKFLMSPT+VKGIMDDIEDEFLYIPGVMTPTEILTAYEAGAEIVKVYPVSALGGIKY
Sbjct: 121 KGGAKFLMSPTMVKGIMDDIEDEFLYIPGVMTPTEILTAYEAGAEIVKVYPVSALGGIKY 180

Query: 181 ISALKKPFPHISMVASQGITIDSIGDYIRQGASSVVLSDAIFNKEFMDQRNFDGIFRLSK 240
           ISALKKPFPHISMVASQGITIDSIGDYIRQGASSVVLSDAIFNKEFMDQRNFDGIFRLSK
Sbjct: 181 ISALKKPFPHISMVASQGITIDSIGDYIRQGASSVVLSDAIFNKEFMDQRNFDGIFRLSK 236

Query: 241 LAASQAMEALEW 253
           LAASQAMEALEW
Sbjct: 241 LAASQAMEALEW 236

[Close]

BLAST of MS004312 vs. ExPASy TrEMBL
Match: A0A6J1H067 (KHG/KDPG aldolase isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111458832 PE=4 SV=1)

HSP 1 Score: 424.5 bits (1090), Expect = 3.1e-115
Identity = 220/252 (87.30%), Postives = 238/252 (94.44%), Query Frame = 0


Query: 1   MLVSRRINMWSLPAIGYCNPFACARFRVFCASSQLSVSPKDKTLKKIYNSGVIACLRASS 60
           MLV+RRI+MWSLPAIG  NPFA ARFRV CASSQL VSPKDKTL+ IYNSGVIACLRASS
Sbjct: 1   MLVARRISMWSLPAIGCWNPFAFARFRVSCASSQLPVSPKDKTLRTIYNSGVIACLRASS 60

Query: 61  AELAMSAACAALNGGVSVLEIVTSTPGVFEVVLQQLLQDYPTKTVGVGTVLNIKDAKDAV 120
           AELAMSAACAALNGGVSVLEIV STPGV E VLQQLLQDYPT+T+GVGTVLN+KDAK+AV
Sbjct: 61  AELAMSAACAALNGGVSVLEIVMSTPGVLE-VLQQLLQDYPTRTLGVGTVLNVKDAKNAV 120

Query: 121 KGGAKFLMSPTLVKGIMDDIEDEFLYIPGVMTPTEILTAYEAGAEIVKVYPVSALGGIKY 180
           + GAKFLMSPT+VKGIMDD+E EFLYIPGVMTPTE+LTAYEAGA+IVKVYPVSALGGIKY
Sbjct: 121 EAGAKFLMSPTMVKGIMDDLEGEFLYIPGVMTPTEVLTAYEAGAQIVKVYPVSALGGIKY 180

Query: 181 ISALKKPFPHISMVASQGITIDSIGDYIRQGASSVVLSDAIFNKEFMDQRNFDGIFRLSK 240
           ISALKKPFPHISMVASQGITI+S GDYIR+GASSVVLSDAIFNKEFM Q+NF+GI +LSK
Sbjct: 181 ISALKKPFPHISMVASQGITIESTGDYIREGASSVVLSDAIFNKEFMKQKNFEGISQLSK 240

Query: 241 LAASQAMEALEW 253
           LAAS+AMEALEW
Sbjct: 241 LAASRAMEALEW 251

[Close]

BLAST of MS004312 vs. ExPASy TrEMBL
Match: A0A1S3BEJ8 (KHG/KDPG aldolase-like OS=Cucumis melo OX=3656 GN=LOC103488815 PE=4 SV=1)

HSP 1 Score: 422.2 bits (1084), Expect = 1.6e-114
Identity = 223/253 (88.14%), Postives = 235/253 (92.89%), Query Frame = 0


Query: 1   MLVSRRINMWSLPAIGYCNPFACARFRVFCASSQLSVSPKDKTLKKIYNSGVIACLRASS 60
           MLVSR I+MWSLP IG  NP A ARFRVFCASSQL V  KDKTL+ I+NSGVIACLRASS
Sbjct: 1   MLVSRSISMWSLPTIGCWNPCAFARFRVFCASSQLPVPSKDKTLRTIHNSGVIACLRASS 60

Query: 61  AELAMSAACAALNGGVSVLEIVTSTPGVFEVVLQQLLQDYPTKTVGVGTVLNIKDAKDAV 120
           AELAMSAACAALNGG+SVLEIV STPGV E VLQQLLQDYPTKT+GVGTVLNIKDAK+AV
Sbjct: 61  AELAMSAACAALNGGISVLEIVMSTPGVLE-VLQQLLQDYPTKTLGVGTVLNIKDAKNAV 120

Query: 121 KGGAKFLMSPTLVKG-IMDDIEDEFLYIPGVMTPTEILTAYEAGAEIVKVYPVSALGGIK 180
           K GAKFLMSPT+VKG IM DIEDEFLYIPGVMTPTE+LTAYE+G EIVKVYPVSALGGIK
Sbjct: 121 KAGAKFLMSPTMVKGIIMGDIEDEFLYIPGVMTPTEVLTAYESGCEIVKVYPVSALGGIK 180

Query: 181 YISALKKPFPHISMVASQGITIDSIGDYIRQGASSVVLSDAIFNKEFMDQRNFDGIFRLS 240
           YISALKKPFPHISMVASQGITI+S GDYIRQGASSVVLSDAIFNKEFMDQ+NFDGIF+LS
Sbjct: 181 YISALKKPFPHISMVASQGITIESTGDYIRQGASSVVLSDAIFNKEFMDQKNFDGIFQLS 240

Query: 241 KLAASQAMEALEW 253
           KLAASQAMEALEW
Sbjct: 241 KLAASQAMEALEW 252

[Close]

BLAST of MS004312 vs. ExPASy TrEMBL
Match: A0A6J1JPL6 (KHG/KDPG aldolase isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111486594 PE=4 SV=1)

HSP 1 Score: 419.9 bits (1078), Expect = 7.8e-114
Identity = 219/252 (86.90%), Postives = 234/252 (92.86%), Query Frame = 0


Query: 1   MLVSRRINMWSLPAIGYCNPFACARFRVFCASSQLSVSPKDKTLKKIYNSGVIACLRASS 60
           MLVSR I+MWSLPAIG  NPFA ARFRV C  SQL VSPKDKTL+ IYNSGVIACLRASS
Sbjct: 1   MLVSRSISMWSLPAIGCWNPFAFARFRVSCTFSQLPVSPKDKTLRTIYNSGVIACLRASS 60

Query: 61  AELAMSAACAALNGGVSVLEIVTSTPGVFEVVLQQLLQDYPTKTVGVGTVLNIKDAKDAV 120
           AELAMSAACAALNGGVSVLEIV STPGV E VLQQLLQDYPT+T+GVGTVLN+KDAK+AV
Sbjct: 61  AELAMSAACAALNGGVSVLEIVMSTPGVLE-VLQQLLQDYPTRTLGVGTVLNVKDAKNAV 120

Query: 121 KGGAKFLMSPTLVKGIMDDIEDEFLYIPGVMTPTEILTAYEAGAEIVKVYPVSALGGIKY 180
           + GAKFLMSPT+V GIMDDIE EFLYIPGVMTPTE+LTAYEAGA+IVKVYPVSALGGIKY
Sbjct: 121 EAGAKFLMSPTMVNGIMDDIEGEFLYIPGVMTPTEVLTAYEAGAQIVKVYPVSALGGIKY 180

Query: 181 ISALKKPFPHISMVASQGITIDSIGDYIRQGASSVVLSDAIFNKEFMDQRNFDGIFRLSK 240
           ISALKKPFPHISMVASQGITI+S GDYIR+GASSVVLSDAIFNKEFM Q+NFDGI +LSK
Sbjct: 181 ISALKKPFPHISMVASQGITIESTGDYIREGASSVVLSDAIFNKEFMKQKNFDGISQLSK 240

Query: 241 LAASQAMEALEW 253
           LAAS+AMEALEW
Sbjct: 241 LAASRAMEALEW 251

The following BLAST results are available for this feature:

BLAST of MS004312 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022139077.1	5.0e-131	98.41	KHG/KDPG aldolase isoform X3 [Momordica charantia] >XP_022139078.1 KHG/KDPG aldo...	[more]
XP_022139079.1	1.8e-120	92.46	KHG/KDPG aldolase isoform X4 [Momordica charantia]	[more]
XP_038892213.1	5.3e-117	89.68	KHG/KDPG aldolase isoform X2 [Benincasa hispida] >XP_038892214.1 KHG/KDPG aldola...	[more]
XP_023511849.1	1.0e-115	88.10	KHG/KDPG aldolase isoform X4 [Cucurbita pepo subsp. pepo]	[more]
XP_022957426.1	6.5e-115	87.30	KHG/KDPG aldolase isoform X2 [Cucurbita moschata] >XP_022957427.1 KHG/KDPG aldol...	[more]

BLAST of MS004312 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P50846	1.6e-15	28.65	KHG/KDPG aldolase OS=Bacillus subtilis (strain 168) OX=224308 GN=kdgA PE=2 SV=1	[more]
Q15X88	1.7e-12	26.92	2-dehydro-3-deoxy-phosphogluconate aldolase OS=Pseudoalteromonas atlantica (stra...	[more]
O68283	3.7e-12	28.34	2-dehydro-3-deoxy-phosphogluconate aldolase OS=Pseudomonas aeruginosa (strain AT...	[more]
O83578	1.1e-11	29.75	Putative KHG/KDPG aldolase OS=Treponema pallidum (strain Nichols) OX=243276 GN=e...	[more]
Q6BF16	1.7e-09	28.16	2-dehydro-3-deoxy-6-phosphogalactonate aldolase OS=Escherichia coli (strain K12)...	[more]

BLAST of MS004312 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CEU6	2.4e-131	98.41	KHG/KDPG aldolase isoform X3 OS=Momordica charantia OX=3673 GN=LOC111010082 PE=4...	[more]
A0A6J1CBW7	8.6e-121	92.46	KHG/KDPG aldolase isoform X4 OS=Momordica charantia OX=3673 GN=LOC111010082 PE=4...	[more]
A0A6J1H067	3.1e-115	87.30	KHG/KDPG aldolase isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111458832 PE=4 ...	[more]
A0A1S3BEJ8	1.6e-114	88.14	KHG/KDPG aldolase-like OS=Cucumis melo OX=3656 GN=LOC103488815 PE=4 SV=1	[more]
A0A6J1JPL6	7.8e-114	86.90	KHG/KDPG aldolase isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111486594 PE=4 SV...	[more]

BLAST of MS004312 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000887	KDPG/KHG aldolase	PFAM	PF01081	Aldolase	coord: 49..238 e-value: 1.3E-32 score: 112.9
IPR000887	KDPG/KHG aldolase	PANTHER	PTHR30246	2-KETO-3-DEOXY-6-PHOSPHOGLUCONATE ALDOLASE	coord: 20..250
IPR000887	KDPG/KHG aldolase	CDD	cd00452	KDPG_aldolase	coord: 49..238 e-value: 7.57131E-49 score: 157.68
IPR013785	Aldolase-type TIM barrel	GENE3D	3.20.20.70	Aldolase class I	coord: 36..250 e-value: 1.6E-61 score: 209.2
None	No IPR available	SUPERFAMILY	51569	Aldolase	coord: 42..242

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS004312.1	MS004312.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0003824	catalytic activity
molecular_function	GO:0016829	lyase activity