MS004276 (gene) Bitter gourd (TR) v1

Overview
NameMS004276
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionPhosphatidylinositol 4-phosphate 5-kinase
Locationscaffold92: 1433645 .. 1437812 (-)
RNA-Seq ExpressionMS004276
SyntenyMS004276
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTACAAAGAATACGCTGGATTCGTGAAGGCTTGGGAAGCAACCGTGAGAAAAACTCAAAGCTCAGCGAAGAAGAGGGCGAATAGCATTTTTGCAGCAATGTCCGTTGCTCCAGCCGACGACGAGCCGGGCCCCGGCGAGCTCCACCACGCGGAGAAGGCGATACCCAATGGCGATTTCTACACCGGACAATGGATGGACAATATGCCCCATGGGCATGGCAAGTACCTGTGGACCGACGGCTGTATGTACGTGGGGGAATGGTACAAGGGGAAGACGTTAGGGAAGGGCAAATTCAGCTGGCCCTCCGGCGCCACCTACGAGGGCGATTTCAAGAGCGGGTATATGGACGGGAAGGGCACTTACACCGGTTCCGCAGGGGACACCTACAGAGGGTGTTGGGTGATGAATTTGAAGCATGGCCAAGGCACCCAAAACTACGCCAATGGAGATTATTATGAGGGGGAATGGCGAAGAGGTTTTCAGGATGGGCAGGGGAGGTATCAATGGAAGAATGAGAATCACTATATAGGGCAGTGGAAGAATGGGAAGATCAATGGCAATGGGACTATGATTTGGAGCAATGGGAATCGGTATGATGGGTGTTGGGAAGATGGATTGCCTAAAGGGAATGGGACATTCAGGTGGGCAGATGGGAGTTGTTTTGTTGGTGTTTGGAGTAAGGATCCTGTAGAGCAGAGTGGGACTTTTTATCCATCTGGGTCTTCTGAGTGTGAAGTGGTCTTGGATCCTCAGGTGGTCTATTCAGAGTATTTTAATGAGTGCATACTTAGTACAGCGGAAAGGATTTTGATCTACCCTTCACAGAAGATGATAAGTTGGTCCGGACTCGACCTCGATTCGCCACAGAAGCAAACAAATTCCAAGAAAGGGTATGATGGGAATAGGAGATTGAGAAGAACATCAGTTGATGGCAAAGTCTGCAATTCTAGTTTTGAATCATCCCATGATAGTTCAAGCGACCTGAGTTACAGAAATGGGACAGAAGGGGGTGGGACTCCTCCGCCCCAAGGTATGGAGAGCAGAGGGAAAAGACAATCAAATTTGAGATTTCAGCCAGCAAAGAGACAGGGTGTTACAATATCAAAGGGACACAAGAACTATGAGCTCATGCTAAATCTGCAGCTAGGGATCAGGTATAAATGATTTAAGAATTTCTTTTTACCTTTTGACTTATGCTGTAGTTTAGCCTTGTCCCTTTTCTGTTGTCTTTTCTTTTCTGCAACTGGATATTAGAGTTTTGCCTTCAGGGCCTGTAACTATGTTCCAGTTCCTATAATGAAACTTAACGACGAGGATATGTTAATGCTACACTTGAAGGCTTGGGCTACCCCCCCAAATTATCTGTGATTCAAATGTAGTCTTATTACCATTATTGTATTTATAAAGTTCCAAACAAATGATATAGCAAGAAATGTACTCTGTAGAGATATTATGTGGCACAATGATCTGTATCATTGTTATTAGCTATGGAACATGAAGTTTGAAGCTGCCTCTACTATTATCTTCATTTCAACTTACTGATTTTTTGTTCCATAGCACAGGCATTCTGTTGGGAGGCCTGCTCCAGCAACATCCCTTGATCTTAAGAATTCGGCTTTCGATCCTAAGGAGAAAGTGTGGACAAAATTTCCACCGGAAGGATCCAAGTATACACCACCTCATCAATCTCACGACTTCAGATGGAAGGATTACTGTCCCGTTGTCTTCAGGTCAGAGCTAAAAGTGAACATCTTTAAATGATTTAAATGAAAGAACCTTTCTGAGACCAAAATTTATTCATGCATAGTAGGAGAACAAGCTTTATTGCTGAATTATACTATCTTACTGGATGGCAGATGTTCATATTCTCTTCAATTTCATTGTCGTTTTGAATTGGAACACTGCATAATCTTATATGGCTCATCAAATTTGTAATTTCAGGACTCTTAGGAAACTGTTCAAGGTGGATCCAGCTGATTACATGCTATCTATATGCGGAAATGATGCCCTTCGGGAACTATCCTCCCCTGGAAAAAGTGGGAGTTTCTTTTATTTAACACATGATGACAGATACATGATTAAAACCATGAAGAAGGCTGAAGTGAAAGTTAGTCCTTTCTTCTCTCTCTCGGCTGTCTCAGATTTGGATTACTCATCTGTGCTTAAAATTAAAATCTCTCCCTAACATAAGAATGTACCAACATCTTATAGAGTTCAAGAACTTCTGATCTCTTGTCTGAATTTGTTTTTACATAATTAGCTTAGTTCCGTAGTCTCAACAAGCCCGTGGTTGTGAATTATGTAACTTTTGCTGAGTTATTAGCACCTCTTTGCAATCATTATGCGGTTATGGAAGCTTGATTCCATTGCATCTACCCTAGGCTTTCCTGATTTCCTCTAGTACGTGTATGCATTTATGTGAGTTGAATATATTCCAATCTTAACAGGCCAAGATACATGCAGGTTCTCTTGCGGATGCTTCCAGCCTACTATAAGCACGTTCGAGCCTTCGAAAATACTCTTGTCACCAAATTTTACGGTCTTCATTGTGTGAGATTAACTGGGACTACCCAAAAAAAGGTATAAAAGGATCACCAAAGCATTACTGCGGTTGCCAAGGATCATTGGATTCACAGATTTTCTTGATCTACAGGTGCGGTTTGTCATAATGGGAAATCTGTTTTGCTCCGAGTATAATATCCAGCGGCGTTTCGACTTGAAAGGTTCTTCTCACGGCCGTACTACTGACAAACCAGAGTCAGAAATCGATGCAACAACTACCCTCAAAGATCTTGATCTTAATTACATATTTCGGTTGCGGAAGATTTGGTTCCAAGAATTTTGTAGGCAAGTATTTCAAAATTGTCCTTACCTGTTATTTGTTACTCATTTCTGTTCATGACAAAGAAGTTGTTTCATTCCTTTATTATCTCATCAATGACCTGCTATAACACATTTAAACTGATACTAACTAGGAGCTATATTATATTGCACGATGGAACCAAAGAAAAAATAAAAATACTTTGTATTGCACAAAAAATTACTAGAGAGATGCAATGTTGCACAAAGAAGTACTGTAGAAAAAAGCTTTACAGCCATTTCACGAGGTTTCATGTCACATTTTTCTTTCCCGGGGGAGAAATGAAACTTCATTAAGAAGAATGAAAGAAGAAAAAAACAGTGGCCTCACAAAAAGGAGCCAAGGTTCAGTTTTTCAGTTTATGCTTCTCATTGCCTGTTCATTGTGTTCCAATGCAGGCAAGTAGACAGGGATTGTGACTTTCTTGAACAGGAGAGAATCATGGACTACAGTCTATTAGTTGGTCTTCACTTCCAGGAAACTTCGTTTCGGGAAGTGGGCACGCCCGATAGCAATCATTCAGGTTTCTGCACTCCAATTGATTATTTCCTAACTATGTTGAGTTTCTTACTCCAAATCATAGTTTCAACAACCAATCTGATGTTTGAAAATGTGATATCTTGCAGGAAGTATAGATCCCGAAAACGAAGCAGATGGTCAATCAGGAGCAGACAAGGAAGAACTTGCTGGTGACTCTACCAGGTAGAAAGAACTCATGAAGTAGTAGTATTCTAATCAATTCCTGAAATCTTGCAGGAACCTTCTCGTTCCACCACTGTGTTCGATATTTTGCTGATGAATCAGTTTTACTTTGCTTTACCATGTTAGCTTTTCGCCTTCTCCAGTGAAATTTCTGAATAATAATGCCTGATTTCTTCAATAGTGAACTGGCTTATTATCATCAATTCAAATAGAAGCTTTCTTGTTTAACATCATCATCATCAATTCAAATATGACCATCTCTGGAAGTAATTGCTCTGAAGTCTGACCATTTGGTTTCTCATGTTATGGACTTTTTTCAGGTGGGCTTCCATTAGATTAGGCATCAACATGCCAGCACGGGCAGAACGGACAGTGAGACGGGGAGAACCCGAAGCTCAGCTAGTAGGAGAGCCCACTGGGGAGTTGTATGATGTGATCATTTTCTTTGGTATAATCGACATACTACAAGACTATGACATCAGCAAAAAACTCGAGCATGCTTACAAATCGTTTCAATACGACCCATCATCGATATCTGCTGTCGATCCAAAGCAATACTCGAAACGCTTTCGAGATTTCGTCTTCAGAATTTTTCTAGAAGAC

mRNA sequence

ATGTACAAAGAATACGCTGGATTCGTGAAGGCTTGGGAAGCAACCGTGAGAAAAACTCAAAGCTCAGCGAAGAAGAGGGCGAATAGCATTTTTGCAGCAATGTCCGTTGCTCCAGCCGACGACGAGCCGGGCCCCGGCGAGCTCCACCACGCGGAGAAGGCGATACCCAATGGCGATTTCTACACCGGACAATGGATGGACAATATGCCCCATGGGCATGGCAAGTACCTGTGGACCGACGGCTGTATGTACGTGGGGGAATGGTACAAGGGGAAGACGTTAGGGAAGGGCAAATTCAGCTGGCCCTCCGGCGCCACCTACGAGGGCGATTTCAAGAGCGGGTATATGGACGGGAAGGGCACTTACACCGGTTCCGCAGGGGACACCTACAGAGGGTGTTGGGTGATGAATTTGAAGCATGGCCAAGGCACCCAAAACTACGCCAATGGAGATTATTATGAGGGGGAATGGCGAAGAGGTTTTCAGGATGGGCAGGGGAGGTATCAATGGAAGAATGAGAATCACTATATAGGGCAGTGGAAGAATGGGAAGATCAATGGCAATGGGACTATGATTTGGAGCAATGGGAATCGGTATGATGGGTGTTGGGAAGATGGATTGCCTAAAGGGAATGGGACATTCAGGTGGGCAGATGGGAGTTGTTTTGTTGGTGTTTGGAGTAAGGATCCTGTAGAGCAGAGTGGGACTTTTTATCCATCTGGGTCTTCTGAGTGTGAAGTGGTCTTGGATCCTCAGGTGGTCTATTCAGAGTATTTTAATGAGTGCATACTTAGTACAGCGGAAAGGATTTTGATCTACCCTTCACAGAAGATGATAAGTTGGTCCGGACTCGACCTCGATTCGCCACAGAAGCAAACAAATTCCAAGAAAGGGTATGATGGGAATAGGAGATTGAGAAGAACATCAGTTGATGGCAAAGTCTGCAATTCTAGTTTTGAATCATCCCATGATAGTTCAAGCGACCTGAGTTACAGAAATGGGACAGAAGGGGGTGGGACTCCTCCGCCCCAAGGTATGGAGAGCAGAGGGAAAAGACAATCAAATTTGAGATTTCAGCCAGCAAAGAGACAGGGTGTTACAATATCAAAGGGACACAAGAACTATGAGCTCATGCTAAATCTGCAGCTAGGGATCAGGCATTCTGTTGGGAGGCCTGCTCCAGCAACATCCCTTGATCTTAAGAATTCGGCTTTCGATCCTAAGGAGAAAGTGTGGACAAAATTTCCACCGGAAGGATCCAAGTATACACCACCTCATCAATCTCACGACTTCAGATGGAAGGATTACTGTCCCGTTGTCTTCAGGACTCTTAGGAAACTGTTCAAGGTGGATCCAGCTGATTACATGCTATCTATATGCGGAAATGATGCCCTTCGGGAACTATCCTCCCCTGGAAAAAGTGGGAGTTTCTTTTATTTAACACATGATGACAGATACATGATTAAAACCATGAAGAAGGCTGAAGTGAAAGTTCTCTTGCGGATGCTTCCAGCCTACTATAAGCACGTTCGAGCCTTCGAAAATACTCTTGTCACCAAATTTTACGGTCTTCATTGTGTGAGATTAACTGGGACTACCCAAAAAAAGGTGCGGTTTGTCATAATGGGAAATCTGTTTTGCTCCGAGTATAATATCCAGCGGCGTTTCGACTTGAAAGGTTCTTCTCACGGCCGTACTACTGACAAACCAGAGTCAGAAATCGATGCAACAACTACCCTCAAAGATCTTGATCTTAATTACATATTTCGGTTGCGGAAGATTTGGTTCCAAGAATTTTGTAGGCAAGTATTTCAAAATTTAGACAGGGATTGTGACTTTCTTGAACAGGAGAGAATCATGGACTACAGTCTATTAGTTGGTCTTCACTTCCAGGAAACTTCGTTTCGGGAAGTGGGCACGCCCGATAGCAATCATTCAGGTTTCTGCACTCCAATTGATTATTTCCTAACTATGTGGGCTTCCATTAGATTAGGCATCAACATGCCAGCACGGGCAGAACGGACAGTGAGACGGGGAGAACCCGAAGCTCAGCTAGTAGGAGAGCCCACTGGGGAGTTGTATGATGTGATCATTTTCTTTGGTATAATCGACATACTACAAGACTATGACATCAGCAAAAAACTCGAGCATGCTTACAAATCGTTTCAATACGACCCATCATCGATATCTGCTGTCGATCCAAAGCAATACTCGAAACGCTTTCGAGATTTCGTCTTCAGAATTTTTCTAGAAGAC

Coding sequence (CDS)

ATGTACAAAGAATACGCTGGATTCGTGAAGGCTTGGGAAGCAACCGTGAGAAAAACTCAAAGCTCAGCGAAGAAGAGGGCGAATAGCATTTTTGCAGCAATGTCCGTTGCTCCAGCCGACGACGAGCCGGGCCCCGGCGAGCTCCACCACGCGGAGAAGGCGATACCCAATGGCGATTTCTACACCGGACAATGGATGGACAATATGCCCCATGGGCATGGCAAGTACCTGTGGACCGACGGCTGTATGTACGTGGGGGAATGGTACAAGGGGAAGACGTTAGGGAAGGGCAAATTCAGCTGGCCCTCCGGCGCCACCTACGAGGGCGATTTCAAGAGCGGGTATATGGACGGGAAGGGCACTTACACCGGTTCCGCAGGGGACACCTACAGAGGGTGTTGGGTGATGAATTTGAAGCATGGCCAAGGCACCCAAAACTACGCCAATGGAGATTATTATGAGGGGGAATGGCGAAGAGGTTTTCAGGATGGGCAGGGGAGGTATCAATGGAAGAATGAGAATCACTATATAGGGCAGTGGAAGAATGGGAAGATCAATGGCAATGGGACTATGATTTGGAGCAATGGGAATCGGTATGATGGGTGTTGGGAAGATGGATTGCCTAAAGGGAATGGGACATTCAGGTGGGCAGATGGGAGTTGTTTTGTTGGTGTTTGGAGTAAGGATCCTGTAGAGCAGAGTGGGACTTTTTATCCATCTGGGTCTTCTGAGTGTGAAGTGGTCTTGGATCCTCAGGTGGTCTATTCAGAGTATTTTAATGAGTGCATACTTAGTACAGCGGAAAGGATTTTGATCTACCCTTCACAGAAGATGATAAGTTGGTCCGGACTCGACCTCGATTCGCCACAGAAGCAAACAAATTCCAAGAAAGGGTATGATGGGAATAGGAGATTGAGAAGAACATCAGTTGATGGCAAAGTCTGCAATTCTAGTTTTGAATCATCCCATGATAGTTCAAGCGACCTGAGTTACAGAAATGGGACAGAAGGGGGTGGGACTCCTCCGCCCCAAGGTATGGAGAGCAGAGGGAAAAGACAATCAAATTTGAGATTTCAGCCAGCAAAGAGACAGGGTGTTACAATATCAAAGGGACACAAGAACTATGAGCTCATGCTAAATCTGCAGCTAGGGATCAGGCATTCTGTTGGGAGGCCTGCTCCAGCAACATCCCTTGATCTTAAGAATTCGGCTTTCGATCCTAAGGAGAAAGTGTGGACAAAATTTCCACCGGAAGGATCCAAGTATACACCACCTCATCAATCTCACGACTTCAGATGGAAGGATTACTGTCCCGTTGTCTTCAGGACTCTTAGGAAACTGTTCAAGGTGGATCCAGCTGATTACATGCTATCTATATGCGGAAATGATGCCCTTCGGGAACTATCCTCCCCTGGAAAAAGTGGGAGTTTCTTTTATTTAACACATGATGACAGATACATGATTAAAACCATGAAGAAGGCTGAAGTGAAAGTTCTCTTGCGGATGCTTCCAGCCTACTATAAGCACGTTCGAGCCTTCGAAAATACTCTTGTCACCAAATTTTACGGTCTTCATTGTGTGAGATTAACTGGGACTACCCAAAAAAAGGTGCGGTTTGTCATAATGGGAAATCTGTTTTGCTCCGAGTATAATATCCAGCGGCGTTTCGACTTGAAAGGTTCTTCTCACGGCCGTACTACTGACAAACCAGAGTCAGAAATCGATGCAACAACTACCCTCAAAGATCTTGATCTTAATTACATATTTCGGTTGCGGAAGATTTGGTTCCAAGAATTTTGTAGGCAAGTATTTCAAAATTTAGACAGGGATTGTGACTTTCTTGAACAGGAGAGAATCATGGACTACAGTCTATTAGTTGGTCTTCACTTCCAGGAAACTTCGTTTCGGGAAGTGGGCACGCCCGATAGCAATCATTCAGGTTTCTGCACTCCAATTGATTATTTCCTAACTATGTGGGCTTCCATTAGATTAGGCATCAACATGCCAGCACGGGCAGAACGGACAGTGAGACGGGGAGAACCCGAAGCTCAGCTAGTAGGAGAGCCCACTGGGGAGTTGTATGATGTGATCATTTTCTTTGGTATAATCGACATACTACAAGACTATGACATCAGCAAAAAACTCGAGCATGCTTACAAATCGTTTCAATACGACCCATCATCGATATCTGCTGTCGATCCAAAGCAATACTCGAAACGCTTTCGAGATTTCGTCTTCAGAATTTTTCTAGAAGAC

Protein sequence

MYKEYAGFVKAWEATVRKTQSSAKKRANSIFAAMSVAPADDEPGPGELHHAEKAIPNGDFYTGQWMDNMPHGHGKYLWTDGCMYVGEWYKGKTLGKGKFSWPSGATYEGDFKSGYMDGKGTYTGSAGDTYRGCWVMNLKHGQGTQNYANGDYYEGEWRRGFQDGQGRYQWKNENHYIGQWKNGKINGNGTMIWSNGNRYDGCWEDGLPKGNGTFRWADGSCFVGVWSKDPVEQSGTFYPSGSSECEVVLDPQVVYSEYFNECILSTAERILIYPSQKMISWSGLDLDSPQKQTNSKKGYDGNRRLRRTSVDGKVCNSSFESSHDSSSDLSYRNGTEGGGTPPPQGMESRGKRQSNLRFQPAKRQGVTISKGHKNYELMLNLQLGIRHSVGRPAPATSLDLKNSAFDPKEKVWTKFPPEGSKYTPPHQSHDFRWKDYCPVVFRTLRKLFKVDPADYMLSICGNDALRELSSPGKSGSFFYLTHDDRYMIKTMKKAEVKVLLRMLPAYYKHVRAFENTLVTKFYGLHCVRLTGTTQKKVRFVIMGNLFCSEYNIQRRFDLKGSSHGRTTDKPESEIDATTTLKDLDLNYIFRLRKIWFQEFCRQVFQNLDRDCDFLEQERIMDYSLLVGLHFQETSFREVGTPDSNHSGFCTPIDYFLTMWASIRLGINMPARAERTVRRGEPEAQLVGEPTGELYDVIIFFGIIDILQDYDISKKLEHAYKSFQYDPSSISAVDPKQYSKRFRDFVFRIFLED
Homology
BLAST of MS004276 vs. NCBI nr
Match: XP_022139196.1 (phosphatidylinositol 4-phosphate 5-kinase 6-like [Momordica charantia])

HSP 1 Score: 1527.3 bits (3953), Expect = 0.0e+00
Identity = 739/766 (96.48%), Postives = 740/766 (96.61%), Query Frame = 0

Query: 1   MYKEYAGFVKAWEATVRKTQSSAKKRANSIFAAMSVAPADDEPGPGELHHAEKAIPNGDF 60
           MYKEYAGFVKAWEATVRKTQSSAKKRANSIFAAMSVAPADDEPGPGELHHAEKAIPNGDF
Sbjct: 1   MYKEYAGFVKAWEATVRKTQSSAKKRANSIFAAMSVAPADDEPGPGELHHAEKAIPNGDF 60

Query: 61  YTGQWMDNMPHGHGKYLWTDGCMYVGEWYKGKTLGKGKFSWPSGATYEGDFKSGYMDGKG 120
           YTGQWMDNMPHGHGKYLWTDGCMYVGEWYKGKTLGKGKFSWPSGATYEGDFKSGYMDGKG
Sbjct: 61  YTGQWMDNMPHGHGKYLWTDGCMYVGEWYKGKTLGKGKFSWPSGATYEGDFKSGYMDGKG 120

Query: 121 TYTGSAGDTYRGCWVMNLKHGQGTQNYANGDYYEGEWRRGFQDGQGRYQWKNENHYIGQW 180
           TYTGSAGDTYRGCWVMNLKHGQGTQNYANGDYYEGEWRRGFQDGQGRYQWKNENHYIGQW
Sbjct: 121 TYTGSAGDTYRGCWVMNLKHGQGTQNYANGDYYEGEWRRGFQDGQGRYQWKNENHYIGQW 180

Query: 181 KNGKINGNGTMIWSNGNRYDGCWEDGLPKGNGTFRWADGSCFVGVWSKDPVEQSGTFYPS 240
           KNGKINGNGTMIWSNGNRYDGCWEDGLPKGNGTFRWADGSCFVGVWSKDPVEQSGTFYPS
Sbjct: 181 KNGKINGNGTMIWSNGNRYDGCWEDGLPKGNGTFRWADGSCFVGVWSKDPVEQSGTFYPS 240

Query: 241 GSSECEVVLDPQVVYSEYFNECILSTAERILIYPSQKMISWSGLDLDSPQKQTNSKKGYD 300
           GSSECEVVLDPQVVYSEYFNECILSTAERILIYPSQKMISWSGLDLDSPQKQTNSKKGYD
Sbjct: 241 GSSECEVVLDPQVVYSEYFNECILSTAERILIYPSQKMISWSGLDLDSPQKQTNSKKGYD 300

Query: 301 GNRRLRRTSVDGKVCNSSFESSHDSSSDLSYRNGTEGGGTPPPQGMESRGKRQSNLRFQP 360
           GNRRLRRTSVDGKVCNSSFESSHDSSSDLSYRNGTEGGGTPPPQGMESRGKRQSNLRFQP
Sbjct: 301 GNRRLRRTSVDGKVCNSSFESSHDSSSDLSYRNGTEGGGTPPPQGMESRGKRQSNLRFQP 360

Query: 361 AKRQGVTISKGHKNYELMLNLQLGIRHSVGRPAPATSLDLKNSAFDPKEKVWTKFPPEGS 420
           AKRQGVTISKGHKNYELMLNLQLGIRHSVGRPAPATSLDLKNSAFDPKEKVWTKFPPEGS
Sbjct: 361 AKRQGVTISKGHKNYELMLNLQLGIRHSVGRPAPATSLDLKNSAFDPKEKVWTKFPPEGS 420

Query: 421 KYTPPHQSHDFRWKDYCPVVFRTLRKLFKVDPADYMLSICGNDALRELSSPGKSGSFFYL 480
           KYTPPHQSHDFRWKDYCPVVFRTLRKLFKVDPADYMLSICGNDALRELSSPGKSGSFFYL
Sbjct: 421 KYTPPHQSHDFRWKDYCPVVFRTLRKLFKVDPADYMLSICGNDALRELSSPGKSGSFFYL 480

Query: 481 THDDRYMIKTMKKAEVKVLLRMLPAYYKHVRAFENTLVTKFYGLHCVRLTGTTQKKVRFV 540
           THDDRYMIKTMKKAEVKVLLRMLPAYYKHVRAFENTLVTKFYGLHCVRLTGTTQKKVRFV
Sbjct: 481 THDDRYMIKTMKKAEVKVLLRMLPAYYKHVRAFENTLVTKFYGLHCVRLTGTTQKKVRFV 540

Query: 541 IMGNLFCSEYNIQRRFDLKGSSHGRTTDKPESEIDATTTLKDLDLNYIFRLRKIWFQEFC 600
           IMGNLFCSEYNIQRRFDLKGSSHGRTTDKPESEIDATTTLKDLDLNYIFRLRKIWFQEFC
Sbjct: 541 IMGNLFCSEYNIQRRFDLKGSSHGRTTDKPESEIDATTTLKDLDLNYIFRLRKIWFQEFC 600

Query: 601 RQVFQNLDRDCDFLEQERIMDYSLLVGLHFQETSFREVGTPDSNHSGFCTP--------- 660
           RQV    DRDCDFLEQERIMDYSLLVGLHFQETSFREVGTPDSNHSG   P         
Sbjct: 601 RQV----DRDCDFLEQERIMDYSLLVGLHFQETSFREVGTPDSNHSGSIDPENEADGQSG 660

Query: 661 -----IDYFLTMWASIRLGINMPARAERTVRRGEPEAQLVGEPTGELYDVIIFFGIIDIL 720
                +    T WASIRLGINMPARAERTVRRGEPEAQLVGEPTGELYDVIIFFGIIDIL
Sbjct: 661 ADKEELAGDSTRWASIRLGINMPARAERTVRRGEPEAQLVGEPTGELYDVIIFFGIIDIL 720

Query: 721 QDYDISKKLEHAYKSFQYDPSSISAVDPKQYSKRFRDFVFRIFLED 753
           QDYDISKKLEHAYKSFQYDPSSISAVDPKQYSKRFRDFVFRIFLED
Sbjct: 721 QDYDISKKLEHAYKSFQYDPSSISAVDPKQYSKRFRDFVFRIFLED 762

BLAST of MS004276 vs. NCBI nr
Match: XP_004142482.1 (phosphatidylinositol 4-phosphate 5-kinase 6 [Cucumis sativus] >KGN52322.1 hypothetical protein Csa_008424 [Cucumis sativus])

HSP 1 Score: 1392.1 bits (3602), Expect = 0.0e+00
Identity = 673/764 (88.09%), Postives = 705/764 (92.28%), Query Frame = 0

Query: 1   MYKEYAGFVKAWEATVRKTQSSAKKRANSIFAAMSVAPADDEPGPGELHHAEKAIPNGDF 60
           MYKEYAG VKAWEATVRKTQ SAKKRANSIFAAMSVAPADDEPGPGELHHAEKAIPNGDF
Sbjct: 1   MYKEYAGIVKAWEATVRKTQHSAKKRANSIFAAMSVAPADDEPGPGELHHAEKAIPNGDF 60

Query: 61  YTGQWMDNMPHGHGKYLWTDGCMYVGEWYKGKTLGKGKFSWPSGATYEGDFKSGYMDGKG 120
           YTGQWMDNMPHGHGKYLWTDGCMYVGEWYKGKTLGKGKFSWPSGATYEGDFKSGYMDGKG
Sbjct: 61  YTGQWMDNMPHGHGKYLWTDGCMYVGEWYKGKTLGKGKFSWPSGATYEGDFKSGYMDGKG 120

Query: 121 TYTGSAGDTYRGCWVMNLKHGQGTQNYANGDYYEGEWRRGFQDGQGRYQWKNENHYIGQW 180
           TYTGS+GDTYRGCWVMNLKHGQGTQNYANGDYYEGEWRRGFQDGQGRYQWKNENHYIGQW
Sbjct: 121 TYTGSSGDTYRGCWVMNLKHGQGTQNYANGDYYEGEWRRGFQDGQGRYQWKNENHYIGQW 180

Query: 181 KNGKINGNGTMIWSNGNRYDGCWEDGLPKGNGTFRWADGSCFVGVWSKDPVEQSGTFYPS 240
           KNGKINGNGTMIW+NGNRYDGCWEDGLPKGNGTFRWADGSC+VGVWSKDP EQ+GTFYPS
Sbjct: 181 KNGKINGNGTMIWNNGNRYDGCWEDGLPKGNGTFRWADGSCYVGVWSKDPTEQTGTFYPS 240

Query: 241 GSSECEVVLDPQVVYSEYFNECILSTAERILIYPSQKMISWSGLDLDSPQKQTNSKKGYD 300
            SSE E+  DPQ +++EY N+ +L + ERILIYPSQKMISWSGLDLDSPQKQT SKKG D
Sbjct: 241 DSSETEMSSDPQEIFAEYLNDFVLCSGERILIYPSQKMISWSGLDLDSPQKQTISKKGND 300

Query: 301 GNRRLRRTSVDGKVCNSSFESSHDSSSDLSYRNGTEGGGTPPPQGMESRGKRQSNLRFQP 360
           GNRRLRRTSVDGKV NSSFES +D S++ S RNG    GT   Q ++S+GKRQSNLRFQP
Sbjct: 301 GNRRLRRTSVDGKVSNSSFESLNDGSNEASSRNGI---GTHQLQDLDSKGKRQSNLRFQP 360

Query: 361 AKRQGVTISKGHKNYELMLNLQLGIRHSVGRPAPATSLDLKNSAFDPKEKVWTKFPPEGS 420
           AKRQG+TISKGHKNYELMLNLQLGIRHSVGRPAPATSLDLK+SAFDPKEKVWTKFPPEGS
Sbjct: 361 AKRQGITISKGHKNYELMLNLQLGIRHSVGRPAPATSLDLKSSAFDPKEKVWTKFPPEGS 420

Query: 421 KYTPPHQSHDFRWKDYCPVVFRTLRKLFKVDPADYMLSICGNDALRELSSPGKSGSFFYL 480
           KYTPPHQS DFRWKDYCPVVFRTLRKLFKVDPADYMLSICGNDALRELSSPGKSGSFFYL
Sbjct: 421 KYTPPHQSSDFRWKDYCPVVFRTLRKLFKVDPADYMLSICGNDALRELSSPGKSGSFFYL 480

Query: 481 THDDRYMIKTMKKAEVKVLLRMLPAYYKHVRAFENTLVTKFYGLHCVRLTGTTQKKVRFV 540
           THDDRYMIKTM+KAEVKVLLRMLPAYYKHVR+FENTLVTKFYGLHCVRLTGT+QKKVRFV
Sbjct: 481 THDDRYMIKTMRKAEVKVLLRMLPAYYKHVRSFENTLVTKFYGLHCVRLTGTSQKKVRFV 540

Query: 541 IMGNLFCSEYNIQRRFDLKGSSHGRTTDKPESEIDATTTLKDLDLNYIFRLRKIWFQEFC 600
           IMGNLFCSEY I RRFDLKGSSHGR TDKPE+EID TTTLKDLDLNYIFRL+KIWFQEFC
Sbjct: 541 IMGNLFCSEYTIHRRFDLKGSSHGRITDKPEAEIDGTTTLKDLDLNYIFRLQKIWFQEFC 600

Query: 601 RQVFQNLDRDCDFLEQERIMDYSLLVGLHFQETSFREVGTPDSNHSGFCTPIDYFL---- 660
           RQV    D+DCDFLEQERIMDYSLLVGLHFQETS+REV TP S+HSG  +  DY      
Sbjct: 601 RQV----DKDCDFLEQERIMDYSLLVGLHFQETSYREVSTPGSHHSG--SNPDYEADGQL 660

Query: 661 --------TMWASIRLGINMPARAERTVRRGEPEAQLVGEPTGELYDVIIFFGIIDILQD 720
                     WASI LGIN+PARAERTVR+GEPEAQLVGEPTGELYDVIIFFGIIDILQD
Sbjct: 661 RADKEQDSARWASISLGINLPARAERTVRQGEPEAQLVGEPTGELYDVIIFFGIIDILQD 720

Query: 721 YDISKKLEHAYKSFQYDPSSISAVDPKQYSKRFRDFVFRIFLED 753
           YDISKKLEHAYKSFQYDPSSISAVDPKQYSKRFRDF+FRIF+ED
Sbjct: 721 YDISKKLEHAYKSFQYDPSSISAVDPKQYSKRFRDFIFRIFVED 755

BLAST of MS004276 vs. NCBI nr
Match: XP_038893257.1 (phosphatidylinositol 4-phosphate 5-kinase 6-like [Benincasa hispida])

HSP 1 Score: 1389.0 bits (3594), Expect = 0.0e+00
Identity = 672/764 (87.96%), Postives = 702/764 (91.88%), Query Frame = 0

Query: 1   MYKEYAGFVKAWEATVRKTQSSAKKRANSIFAAMSVAPADDEPGPGELHHAEKAIPNGDF 60
           MYKEYAG VKAWEATVRKTQ+SAKKRANSIFAAMSVAPADDEPGPGELHHAEKAIPNGDF
Sbjct: 1   MYKEYAGIVKAWEATVRKTQNSAKKRANSIFAAMSVAPADDEPGPGELHHAEKAIPNGDF 60

Query: 61  YTGQWMDNMPHGHGKYLWTDGCMYVGEWYKGKTLGKGKFSWPSGATYEGDFKSGYMDGKG 120
           YTGQWMDNMPHGHGKYLWTDGCMYVGEWYKGKTLGKGKFSWPSGATYEGDFKSGYMDGKG
Sbjct: 61  YTGQWMDNMPHGHGKYLWTDGCMYVGEWYKGKTLGKGKFSWPSGATYEGDFKSGYMDGKG 120

Query: 121 TYTGSAGDTYRGCWVMNLKHGQGTQNYANGDYYEGEWRRGFQDGQGRYQWKNENHYIGQW 180
           TYTGS+GDTYRGCWVMNLKHGQGTQNYANGDYYEGEWRRGFQDGQGRYQWKNENHYIGQW
Sbjct: 121 TYTGSSGDTYRGCWVMNLKHGQGTQNYANGDYYEGEWRRGFQDGQGRYQWKNENHYIGQW 180

Query: 181 KNGKINGNGTMIWSNGNRYDGCWEDGLPKGNGTFRWADGSCFVGVWSKDPVEQSGTFYPS 240
           KNGKINGNGTMIW+NGNRYDGCWEDGLP+GNGTFRWADGSC+VGVWSKDP EQ+GTFYPS
Sbjct: 181 KNGKINGNGTMIWNNGNRYDGCWEDGLPRGNGTFRWADGSCYVGVWSKDPTEQTGTFYPS 240

Query: 241 GSSECEVVLDPQVVYSEYFNECILSTAERILIYPSQKMISWSGLDLDSPQKQTNSKKGYD 300
            SSE E+  DPQ +++EY N+ +L   ERILIYPSQKMI+WSGLDLDS QKQT SKKG D
Sbjct: 241 DSSETEMSSDPQEIFAEYLNDFVLCAGERILIYPSQKMITWSGLDLDSSQKQTVSKKGND 300

Query: 301 GNRRLRRTSVDGKVCNSSFESSHDSSSDLSYRNGTEGGGTPPPQGMESRGKRQSNLRFQP 360
           GNRRLRRTSVDGKV NSSFES +D S++ S RNG    GT   Q ++S+GKRQ NLRFQP
Sbjct: 301 GNRRLRRTSVDGKVSNSSFESLNDGSNEASSRNGK---GTHLLQDLDSKGKRQPNLRFQP 360

Query: 361 AKRQGVTISKGHKNYELMLNLQLGIRHSVGRPAPATSLDLKNSAFDPKEKVWTKFPPEGS 420
           AKRQGVTISKGHKNYELMLNLQLGIRHSVGRPAPATSLDLK+SAFDPKEKVWTKFPPEGS
Sbjct: 361 AKRQGVTISKGHKNYELMLNLQLGIRHSVGRPAPATSLDLKSSAFDPKEKVWTKFPPEGS 420

Query: 421 KYTPPHQSHDFRWKDYCPVVFRTLRKLFKVDPADYMLSICGNDALRELSSPGKSGSFFYL 480
           KYTPPHQS DFRWKDYCPVVFRTLRKLFKVDPADYMLSICGNDALRELSSPGKSGSFFYL
Sbjct: 421 KYTPPHQSSDFRWKDYCPVVFRTLRKLFKVDPADYMLSICGNDALRELSSPGKSGSFFYL 480

Query: 481 THDDRYMIKTMKKAEVKVLLRMLPAYYKHVRAFENTLVTKFYGLHCVRLTGTTQKKVRFV 540
           THDDRYMIKTMKKAEVKVLLRMLPAYYKHVR+FENTLVTKFYGLHCVRLTGT+QKKVRFV
Sbjct: 481 THDDRYMIKTMKKAEVKVLLRMLPAYYKHVRSFENTLVTKFYGLHCVRLTGTSQKKVRFV 540

Query: 541 IMGNLFCSEYNIQRRFDLKGSSHGRTTDKPESEIDATTTLKDLDLNYIFRLRKIWFQEFC 600
           IMGNLFCSEY I RRFDLKGSSHGR TDKPE+EID TTTLKDLDLNYIFRL+KIWFQEFC
Sbjct: 541 IMGNLFCSEYTIHRRFDLKGSSHGRITDKPEAEIDGTTTLKDLDLNYIFRLQKIWFQEFC 600

Query: 601 RQVFQNLDRDCDFLEQERIMDYSLLVGLHFQETSFREVGTPDSNHSGFCTPIDYFL---- 660
           RQV    D+DCDFLEQERIMDYSLLVGLHFQETS+RE  TP+S+HSG     DY      
Sbjct: 601 RQV----DKDCDFLEQERIMDYSLLVGLHFQETSYREASTPESHHSG-SRDTDYEADGQL 660

Query: 661 --------TMWASIRLGINMPARAERTVRRGEPEAQLVGEPTGELYDVIIFFGIIDILQD 720
                     WASI LGINMPARAERTVRRGEPEAQLVGEPTGELYDVIIFFGIIDILQD
Sbjct: 661 RADKAQDSARWASISLGINMPARAERTVRRGEPEAQLVGEPTGELYDVIIFFGIIDILQD 720

Query: 721 YDISKKLEHAYKSFQYDPSSISAVDPKQYSKRFRDFVFRIFLED 753
           YDISKKLEHAYKSFQYDPSSISAVDPKQYSKRFRDF+FRIF+ED
Sbjct: 721 YDISKKLEHAYKSFQYDPSSISAVDPKQYSKRFRDFIFRIFVED 756

BLAST of MS004276 vs. NCBI nr
Match: KAA0034762.1 (phosphatidylinositol 4-phosphate 5-kinase 6 [Cucumis melo var. makuwa])

HSP 1 Score: 1383.6 bits (3580), Expect = 0.0e+00
Identity = 672/786 (85.50%), Postives = 705/786 (89.69%), Query Frame = 0

Query: 1   MYKEYAGFVKAWEATVRKTQSSAKKRANSIFAAMSVAPADDEPGPGELHHAEKAIPNGDF 60
           MYKEYAG VKAWEATVRKTQ+SAKKRANSIFAAMSVAPADDEPGPGELHHAEKAIPNGDF
Sbjct: 1   MYKEYAGIVKAWEATVRKTQNSAKKRANSIFAAMSVAPADDEPGPGELHHAEKAIPNGDF 60

Query: 61  YTGQWMDNMPHGHGKYLWTDGCMYVGEWYKGKTLGKGKFSWPSGATYEGDFKSGYMDGKG 120
           YTGQWMD+MPHGHGKYLWTDGCMYVGEWYKGKTLGKGKFSWPSGATYEGDFKSGYMDGKG
Sbjct: 61  YTGQWMDHMPHGHGKYLWTDGCMYVGEWYKGKTLGKGKFSWPSGATYEGDFKSGYMDGKG 120

Query: 121 TYTGSAGDTYRGCWVMNLKHGQGTQNYANGDYYEGEWRRGFQDGQGRYQWKNENHYIGQW 180
           TYTGS+GDTYRGCWVMNLKHGQGTQNYANGDYY+GEWRRGFQDGQGRYQWKNENHYIGQW
Sbjct: 121 TYTGSSGDTYRGCWVMNLKHGQGTQNYANGDYYDGEWRRGFQDGQGRYQWKNENHYIGQW 180

Query: 181 KNGKINGNGTMIWSNGNRYDGCWEDGLPKGNGTFRWADGSCFVGVWSKDPVEQSGTFYPS 240
           KNGKINGNGTMIW+NGNRYDGCWEDGLPKGNGTFRWADGSC+VGVWSKDP EQ+GTFYPS
Sbjct: 181 KNGKINGNGTMIWNNGNRYDGCWEDGLPKGNGTFRWADGSCYVGVWSKDPTEQTGTFYPS 240

Query: 241 GSSECEVVLDPQVVYSEYFNECILSTAERILIYPSQKMISWSGLDLDSPQKQTNSKKGYD 300
            SSE E+  DPQ +++EY N+ +L + ERILIYPSQKMISWSGLDLDSPQKQT SKKG D
Sbjct: 241 DSSEAEMSSDPQEIFAEYLNDFVLCSGERILIYPSQKMISWSGLDLDSPQKQTISKKGND 300

Query: 301 GNRRLRRTSVDGKVCNSSFESSHDSSSDLSYRNGTEGGGTPPPQGMESRGKRQSNLRFQP 360
           GNRRLRRTSVDGKV NSSFES +D S++ S RNG    GT   Q ++S+GKRQSNLRFQP
Sbjct: 301 GNRRLRRTSVDGKVSNSSFESLNDGSNEASSRNGI---GTHHLQDLDSKGKRQSNLRFQP 360

Query: 361 AKRQGVTISKGHKNYELMLNLQLGIRHSVGRPAPATSLDLKNSAFDPKEKVWTKFPPEGS 420
           AKRQGVTISKGHKNYELMLNLQLGIRHSVGRPAPATSLDLK+SAFDPKEKVWTKFPPEGS
Sbjct: 361 AKRQGVTISKGHKNYELMLNLQLGIRHSVGRPAPATSLDLKSSAFDPKEKVWTKFPPEGS 420

Query: 421 KYTPPHQSHDFRWKDYCPVVFRTLRKLFKVDPADYMLSICGNDALRELSSPGKSGSFFYL 480
           KYTPPHQS DFRWKDYCPVVFRTLRKLFKVDPADYMLSICGNDALRELSSPGKSGSFFYL
Sbjct: 421 KYTPPHQSSDFRWKDYCPVVFRTLRKLFKVDPADYMLSICGNDALRELSSPGKSGSFFYL 480

Query: 481 THDDRYMIKTMKKAEVKVLLRMLPAYYKHVRAFENTLVTKFYGLHCVRLTGTTQKK---- 540
           THDDRYMIKTM+KAEVKVLLRMLPAYYKHVR+FENTLVTKFYGLHCVRLTGT+QKK    
Sbjct: 481 THDDRYMIKTMRKAEVKVLLRMLPAYYKHVRSFENTLVTKFYGLHCVRLTGTSQKKVYKD 540

Query: 541 --------------------VRFVIMGNLFCSEYNIQRRFDLKGSSHGRTTDKPESEIDA 600
                               VRFVIMGNLFCSEY I RRFDLKGSSHGR TDKPE+EID 
Sbjct: 541 TKAVQWLPWILQFTDFVDLQVRFVIMGNLFCSEYTIHRRFDLKGSSHGRITDKPEAEIDG 600

Query: 601 TTTLKDLDLNYIFRLRKIWFQEFCRQVFQNLDRDCDFLEQERIMDYSLLVGLHFQETSFR 660
           TTTLKDLDLNYIFRL+KIWFQEFCRQV    D+DCDFLEQERIMDYSLLVGLHFQETS+R
Sbjct: 601 TTTLKDLDLNYIFRLQKIWFQEFCRQV----DKDCDFLEQERIMDYSLLVGLHFQETSYR 660

Query: 661 EVGTPDSNHSGFCTPID----------YFLTMWASIRLGINMPARAERTVRRGEPEAQLV 720
           E  TP+S+HSGFC   D               WASI LGIN+PARAERTVRR EPEAQLV
Sbjct: 661 EASTPESHHSGFCCLFDSNGQLRANKEQDSARWASISLGINLPARAERTVRRAEPEAQLV 720

Query: 721 GEPTGELYDVIIFFGIIDILQDYDISKKLEHAYKSFQYDPSSISAVDPKQYSKRFRDFVF 753
           GEPTGELYDVIIFFGIIDILQDYDISKKLEHAYKSFQYDPSSISAVDPKQYSKRFRDF+F
Sbjct: 721 GEPTGELYDVIIFFGIIDILQDYDISKKLEHAYKSFQYDPSSISAVDPKQYSKRFRDFIF 779

BLAST of MS004276 vs. NCBI nr
Match: TYK09316.1 (phosphatidylinositol 4-phosphate 5-kinase 6 isoform X2 [Cucumis melo var. makuwa])

HSP 1 Score: 1375.5 bits (3559), Expect = 0.0e+00
Identity = 671/788 (85.15%), Postives = 705/788 (89.47%), Query Frame = 0

Query: 1   MYKEYAGFVKAWEATVRKTQSSAKKRANSIFAAMSVAPADDEPGPGELHHAEKAIPNGDF 60
           MYKEYAG VKAWEATVRKTQ+SAKKRANSIFAAMSVAPADDEPGPGELHHAEKAIPNGDF
Sbjct: 1   MYKEYAGIVKAWEATVRKTQNSAKKRANSIFAAMSVAPADDEPGPGELHHAEKAIPNGDF 60

Query: 61  YTGQWMDNMPHGHGKYLWTDGCMYVGEWYKGKTLGKGKFSWPSGATYEGDFKSGYMDGKG 120
           YTGQWMD+MPHGHGKYLWTDGCMYVGEWYKGKTLGKGKFSWPSGATYEGDFKSGYMDGKG
Sbjct: 61  YTGQWMDHMPHGHGKYLWTDGCMYVGEWYKGKTLGKGKFSWPSGATYEGDFKSGYMDGKG 120

Query: 121 TYTGSAGDTYRGCWVMNLKHGQGTQNYANGDYYEGEWRRGFQDGQGRYQWKNENHYIGQW 180
           TYTGS+GDTYRGCWVMNLKHGQGTQNYANGDYY+GEWRRGFQDGQGRYQWKNENHYIGQW
Sbjct: 121 TYTGSSGDTYRGCWVMNLKHGQGTQNYANGDYYDGEWRRGFQDGQGRYQWKNENHYIGQW 180

Query: 181 KNGKINGNGTMIWSNGNRYDGCWEDGLPKGNGTFRWADGSCFVGVWSKDPVEQSGTFYPS 240
           KNGKINGNGTMIW+NGNRYDGCWEDGLPKGNGTFRWADGSC+VGVWSKDP EQ+GTFYPS
Sbjct: 181 KNGKINGNGTMIWNNGNRYDGCWEDGLPKGNGTFRWADGSCYVGVWSKDPTEQTGTFYPS 240

Query: 241 GSSECEVVLDPQVVYSEYFNECILSTAERILIYPSQKMISWSGLDLDSPQKQTNSKKGYD 300
            SSE E+  DPQ +++EY N+ +L + ERILIYPSQKMISWSGLDLDSPQKQT SKKG D
Sbjct: 241 DSSEAEMSSDPQEIFAEYLNDFVLCSGERILIYPSQKMISWSGLDLDSPQKQTISKKGND 300

Query: 301 GNRRLRRTSVDGKVCNSSFESSHDSSSDLSYRNGTEGGGTPPPQGMESRGKRQSNLRFQP 360
           GNRRLRRTSVDGKV NSSFES +D S++ S RNG    GT   Q ++S+GKRQSNLRFQP
Sbjct: 301 GNRRLRRTSVDGKVSNSSFESLNDGSNEASSRNGI---GTHHLQDLDSKGKRQSNLRFQP 360

Query: 361 AKRQGVTISKGHKNYELMLNLQLGIRHSVGRPAPATSLDLKNSAFDPKEKVWTKFPPEGS 420
           AKRQGVTISKGHKNYELMLNLQLGIRHSVGRPAPATSLDLK+SAFDPKEKVWTKFPPEGS
Sbjct: 361 AKRQGVTISKGHKNYELMLNLQLGIRHSVGRPAPATSLDLKSSAFDPKEKVWTKFPPEGS 420

Query: 421 KYTPPHQSHDFRWKDYCPVVFRTLRKLFKVDPADYMLSICGNDALRELSSPGKSGSFFYL 480
           KYTPPHQS DFRWKDYCPVVFRTLRKLFKVDPADYMLSICGNDALRELSSPGKSGSFFYL
Sbjct: 421 KYTPPHQSSDFRWKDYCPVVFRTLRKLFKVDPADYMLSICGNDALRELSSPGKSGSFFYL 480

Query: 481 THDDRYMIKTMKKAEVKVLLRMLPAYYKHVRAFENTLVTKFYGLHCVRLTGTTQKK---- 540
           THDDRYMIKTM+KAEVKVLLRMLPAYYKHVR+FENTLVTKFYGLHCVRLTGT+QKK    
Sbjct: 481 THDDRYMIKTMRKAEVKVLLRMLPAYYKHVRSFENTLVTKFYGLHCVRLTGTSQKKVYKD 540

Query: 541 --------------------VRFVIMGNLFCSEYNIQRRFDLKGSSHGRTTDKPESEIDA 600
                               VRFVIMGNLFCSEY I RRFDLKGSSHGR TDKPE+EID 
Sbjct: 541 TKAVQWLPWILQFTDFVDLQVRFVIMGNLFCSEYTIHRRFDLKGSSHGRITDKPEAEIDG 600

Query: 601 TTTLKDLDLNYIFRLRKIWFQEFCRQVFQNLDRDCDFLEQERIMDYSLLVGLHFQETSFR 660
           TTTLKDLDLNYIFRL+KIWFQEFCRQV    D+DCDFLEQERIMDYSLLVGLHFQETS+R
Sbjct: 601 TTTLKDLDLNYIFRLQKIWFQEFCRQV----DKDCDFLEQERIMDYSLLVGLHFQETSYR 660

Query: 661 EVGTPDSNHSGFCTPIDYFL------------TMWASIRLGINMPARAERTVRRGEPEAQ 720
           E  TP+S+HSG  +  DY                WASI LGIN+PARAERTVRR EPEAQ
Sbjct: 661 EASTPESHHSG--SNPDYEADGQLRANKEQDSARWASISLGINLPARAERTVRRAEPEAQ 720

Query: 721 LVGEPTGELYDVIIFFGIIDILQDYDISKKLEHAYKSFQYDPSSISAVDPKQYSKRFRDF 753
           LVGEPTGELYDVIIFFGIIDILQDYDISKKLEHAYKSFQYDPSSISAVDPKQYSKRFRDF
Sbjct: 721 LVGEPTGELYDVIIFFGIIDILQDYDISKKLEHAYKSFQYDPSSISAVDPKQYSKRFRDF 779

BLAST of MS004276 vs. ExPASy Swiss-Prot
Match: Q9SFB8 (Phosphatidylinositol 4-phosphate 5-kinase 6 OS=Arabidopsis thaliana OX=3702 GN=PIP5K6 PE=2 SV=1)

HSP 1 Score: 1055.4 bits (2728), Expect = 2.9e-307
Identity = 524/738 (71.00%), Postives = 587/738 (79.54%), Query Frame = 0

Query: 34  MSVAPADDEPG----PGELHHAEKAIPNGDFYTGQWMDNMPHGHGKYLWTDGCMYVGEWY 93
           MSVA ADD        GE +HAEKA+P+GDFYTGQW DN+PHGHGKYLWTDGCMYVG+W+
Sbjct: 1   MSVAHADDADDYSRPTGESYHAEKALPSGDFYTGQWRDNLPHGHGKYLWTDGCMYVGDWH 60

Query: 94  KGKTLGKGKFSWPSGATYEGDFKSGYMDGKGTYTGSAGDTYRGCWVMNLKHGQGTQNYAN 153
           +GKT+GKG+FSWPSGATYEGDFK+GYMDGKGTY  S+GD YRG WVMNL+HGQGT++Y N
Sbjct: 61  RGKTMGKGRFSWPSGATYEGDFKNGYMDGKGTYIDSSGDLYRGSWVMNLRHGQGTKSYVN 120

Query: 154 GDYYEGEWRRGFQDGQGRYQWKNENHYIGQWKNGKINGNGTMIWSNGNRYDGCWEDGLPK 213
           GD Y+GEWRRG QDG GRYQWKNENHYIGQWKNG +NGNGTMIWSNGNRYDG WEDG PK
Sbjct: 121 GDCYDGEWRRGLQDGHGRYQWKNENHYIGQWKNGLMNGNGTMIWSNGNRYDGSWEDGAPK 180

Query: 214 GNGTFRWADGSCFVGVWSKDPVEQSGTFYPSGSSECEVVLDPQVVYSEYFNECILSTAER 273
           GNGTFRW+DGS +VGVWSKDP EQ+GT+YPS SS       PQ V+    +EC++ T +R
Sbjct: 181 GNGTFRWSDGSFYVGVWSKDPKEQNGTYYPSTSSG-NFDWQPQQVFYVDLSECVVCTCQR 240

Query: 274 ILIYPSQKMISWSGLDLDSPQKQTNSKKGYDGNRRLRRTSVDGKVCNSSFESSHDSSSDL 333
           I + PSQKM  W G       +Q++S      + R RR SVDG+V N   E   + S  L
Sbjct: 241 IPVLPSQKMPVWYG-----ASEQSSSGNRTKNSERPRRRSVDGRVSNGEMELRSNGSGYL 300

Query: 334 SYRNGTEGGGTPPPQGMESRGKRQSNLRFQPAKRQGVTISKGHKNYELMLNLQLGIRHSV 393
              +             ES       LR QPAK+QG TISKGHKNYELMLNLQLGIRHSV
Sbjct: 301 QVDDNA-----------ESTRSSLGPLRIQPAKKQGQTISKGHKNYELMLNLQLGIRHSV 360

Query: 394 GRPAPATSLDLKNSAFDPKEKVWTKFPPEGSKYTPPHQSHDFRWKDYCPVVFRTLRKLFK 453
           GRPAPATSLDLK SAFDPKEK+WTKFP EGSKYTPPHQS +F+WKDYCPVVFRTLRKLF 
Sbjct: 361 GRPAPATSLDLKASAFDPKEKLWTKFPSEGSKYTPPHQSCEFKWKDYCPVVFRTLRKLFS 420

Query: 454 VDPADYMLSICGNDALRELSSPGKSGSFFYLTHDDRYMIKTMKKAEVKVLLRMLPAYYKH 513
           VD ADYMLSICGNDALRELSSPGKSGSFFYLT+DDRYMIKTMKKAE KVL+RMLPAYY H
Sbjct: 421 VDAADYMLSICGNDALRELSSPGKSGSFFYLTNDDRYMIKTMKKAETKVLIRMLPAYYNH 480

Query: 514 VRAFENTLVTKFYGLHCVRLTGTTQKKVRFVIMGNLFCSEYNIQRRFDLKGSSHGRTTDK 573
           VRA ENTLVTKF+GLHCV+LTGT QKKVRFVIMGNLFC+ ++I RRFDLKGSSHGR T K
Sbjct: 481 VRACENTLVTKFFGLHCVKLTGTAQKKVRFVIMGNLFCTGHSIHRRFDLKGSSHGRLTTK 540

Query: 574 PESEIDATTTLKDLDLNYIFRLRKIWFQEFCRQVFQNLDRDCDFLEQERIMDYSLLVGLH 633
           PESEID  TTLKDLDLN+ FRL+K WFQEFCRQV    DRDC+FLEQERIMDYSLLVGLH
Sbjct: 541 PESEIDPNTTLKDLDLNFAFRLQKNWFQEFCRQV----DRDCEFLEQERIMDYSLLVGLH 600

Query: 634 FQETSFREVGTPDSNHSGFCTP------------IDYFL---TMWASIRLGINMPARAER 693
           F+E + ++  TP    SG  TP            +D FL   +  ASI+LGINMPAR ER
Sbjct: 601 FREAAIKDSATPT---SGARTPTGNSETRLSRAEMDRFLLDASKLASIKLGINMPARVER 660

Query: 694 TVRRGEPEAQLVGEPTGELYDVIIFFGIIDILQDYDISKKLEHAYKSFQYDPSSISAVDP 753
           T RR + E QLVG+PTGE YDVI++FGIIDILQDYDISKKLEHAYKS QYDP+SISAVDP
Sbjct: 661 TARRSDCENQLVGDPTGEFYDVIVYFGIIDILQDYDISKKLEHAYKSMQYDPTSISAVDP 714

BLAST of MS004276 vs. ExPASy Swiss-Prot
Match: Q9M1K2 (Phosphatidylinositol 4-phosphate 5-kinase 4 OS=Arabidopsis thaliana OX=3702 GN=PIP5K4 PE=4 SV=1)

HSP 1 Score: 984.2 bits (2543), Expect = 8.3e-286
Identity = 506/807 (62.70%), Postives = 600/807 (74.35%), Query Frame = 0

Query: 1   MYKEYAGFVKAWEATVRKTQSSAKKRANSIFAAMSVAPADDEPGP--------------- 60
           M KE +  +KAWE TVRKTQ  AKKRANSIF  +SVAP  D+                  
Sbjct: 1   MSKEQSCVLKAWEVTVRKTQ-QAKKRANSIFGTVSVAPQTDDDATTTTEENDDETSTNRS 60

Query: 61  --GELHHAEKAIPNGDFYTGQWMDNMPHGHGKYLWTDGCMYVGEWYKGKTLGKGKFSWPS 120
             GEL+HAE+ +PNGD+YTGQW D+ PHGHGKYLWTDGCMY+G+WY GKT+G+GKF WPS
Sbjct: 61  SIGELYHAERILPNGDYYTGQWYDSFPHGHGKYLWTDGCMYIGDWYNGKTMGRGKFGWPS 120

Query: 121 GATYEGDFKSGYMDGKGTYTGSAGDTYRGCWVMNLKHGQGTQNYANGDYYEGEWRRGFQD 180
           GATYEG+FKSGYMDG G YTG +GDTY+G WVMNLKHG G + +ANGD Y+GEWRRG Q+
Sbjct: 121 GATYEGEFKSGYMDGVGLYTGPSGDTYKGQWVMNLKHGHGIKRFANGDVYDGEWRRGLQE 180

Query: 181 GQGRYQWKNENHYIGQWKNGKINGNGTMIWSNGNRYDGCWEDGLPKGNGTFRWADGSCFV 240
            QG+YQW++ ++Y+G+WKN  I G GT IW++GNRYDG W+DG P+GNGTF+WADGS +V
Sbjct: 181 AQGKYQWRDGSYYMGEWKNATICGKGTFIWTDGNRYDGFWDDGFPRGNGTFKWADGSFYV 240

Query: 241 GVWSKDPVEQSGTFYPSGSSECEVVLDPQVVYSEYFNECILSTAERILIYPSQKMIS-WS 300
           G WS DP E +GT+YPSG  +     DP+ V++   +E  + + ER+ + PSQK +S W 
Sbjct: 241 GHWSNDPEEMNGTYYPSG-DDGSPEWDPKDVFTN-LSEYKICSGERVPVLPSQKKLSVW- 300

Query: 301 GLDLDSPQKQTNSKKGYDGNRRLRRTSVDGKV---CNSSFE-------SSHDSSSDLSYR 360
                      NS K  +   + RR SVDG+V    + +FE        S + ++D+   
Sbjct: 301 -----------NSSKRVE---KPRRMSVDGRVSVGVDRAFEKMNMWGTESGEGAADID-- 360

Query: 361 NGTEGGGTPPPQGMESRGKRQSNLRFQPA--------KRQGVTISKGHKNYELMLNLQLG 420
           + T          +E+ G  QS LR  PA        ++QG TISKGH+NYELMLNLQLG
Sbjct: 361 STTRRDLDAEMMRLEAEGFIQS-LRPSPAPMRLPRAGRKQGETISKGHRNYELMLNLQLG 420

Query: 421 IRHSVGRPAPATSLDLKNSAFDPKEKVWTKFPPEGSKYTPPHQSHDFRWKDYCPVVFRTL 480
           IRH+VG+ AP  SLDLK+SAFDPKEKVWT+FPPEG+KYTPPHQS +F+WKDYCP+VFR+L
Sbjct: 421 IRHAVGKQAPVVSLDLKHSAFDPKEKVWTRFPPEGTKYTPPHQSSEFKWKDYCPLVFRSL 480

Query: 481 RKLFKVDPADYMLSICGNDALRELSSPGKSGSFFYLTHDDRYMIKTMKKAEVKVLLRMLP 540
           RKLFKVDPADYMLSICGNDALRELSSPGKSGSFFYLT+DDRYMIKTMKK+E KVLLRML 
Sbjct: 481 RKLFKVDPADYMLSICGNDALRELSSPGKSGSFFYLTNDDRYMIKTMKKSETKVLLRMLA 540

Query: 541 AYYKHVRAFENTLVTKFYGLHCVRLTGTTQKKVRFVIMGNLFCSEYNIQRRFDLKGSSHG 600
           AYY HVRAFENTLV +FYGLHCV+LTG  QKKVRFVIMGNLFCSEY+I RRFDLKGSS G
Sbjct: 541 AYYNHVRAFENTLVIRFYGLHCVKLTGPIQKKVRFVIMGNLFCSEYSIHRRFDLKGSSLG 600

Query: 601 RTTDKPESEIDATTTLKDLDLNYIFRLRKIWFQEFCRQVFQNLDRDCDFLEQERIMDYSL 660
           RTTDKPESEI++ T LKDLDLN+IFRL+K W+QEF RQV    D+DC+FLEQERIMDYSL
Sbjct: 601 RTTDKPESEINSNTILKDLDLNFIFRLQKAWYQEFIRQV----DKDCEFLEQERIMDYSL 660

Query: 661 LVGLHFQETSFREVGTPDSNHSGFCTPIDYF-------------------LTMWASIRLG 720
           LVG+HF+E S      P    SG  TPI  F                    T WASIRLG
Sbjct: 661 LVGIHFREASVAGELIP----SGARTPIGEFEDESAPRLSRADVDQLLSDPTRWASIRLG 720

Query: 721 INMPARAERTVRRGEPEAQLVGEPTGELYDVIIFFGIIDILQDYDISKKLEHAYKSFQYD 753
            NMPARAERT+RR + E QLVGEPTGE Y+V++ FGIIDILQDYDISKKLEHAYKS QYD
Sbjct: 721 GNMPARAERTMRRSDCEFQLVGEPTGEYYEVVMIFGIIDILQDYDISKKLEHAYKSIQYD 778

BLAST of MS004276 vs. ExPASy Swiss-Prot
Match: Q9SLG9 (Phosphatidylinositol 4-phosphate 5-kinase 5 OS=Arabidopsis thaliana OX=3702 GN=PIP5K5 PE=2 SV=1)

HSP 1 Score: 972.6 bits (2513), Expect = 2.5e-282
Identity = 494/802 (61.60%), Postives = 598/802 (74.56%), Query Frame = 0

Query: 1   MYKEYAGFVKAWEATVRKTQSSAKKRANSIFAAMSVAP----------ADDEPGPG---- 60
           M K+ +  +KAWE TVRKTQ  AKKRANS+F  +SVAP           DDE        
Sbjct: 1   MSKDQSYVLKAWEVTVRKTQ-QAKKRANSVFGTVSVAPHTDNDTTTDDNDDETTTNRSSL 60

Query: 61  -ELHHAEKAIPNGDFYTGQWMDNMPHGHGKYLWTDGCMYVGEWYKGKTLGKGKFSWPSGA 120
            E +HAE+ +PNGD+YTGQW D+ PHGHGKYLWTDGCMY+G+WY GKT+G GKF WPSGA
Sbjct: 61  EEFYHAERVLPNGDYYTGQWYDSFPHGHGKYLWTDGCMYIGDWYNGKTMGNGKFGWPSGA 120

Query: 121 TYEGDFKSGYMDGKGTYTGSAGDTYRGCWVMNLKHGQGTQNYANGDYYEGEWRRGFQDGQ 180
           TYEG+FKSGYMDG GTYTG +GD Y+G WVMNLKHG G +++ANGD Y+GEWRRG Q+GQ
Sbjct: 121 TYEGEFKSGYMDGIGTYTGPSGDAYKGQWVMNLKHGHGVKSFANGDAYDGEWRRGLQEGQ 180

Query: 181 GRYQWKNENHYIGQWKNGKINGNGTMIWSNGNRYDGCWEDGLPKGNGTFRWADGSCFVGV 240
           G+YQW + ++YIG+WKNG I G G+ +W+NGNRYDG W++G P+GNGTF+W +GS +VG 
Sbjct: 181 GKYQWSDGSYYIGEWKNGTICGKGSFVWTNGNRYDGFWDEGFPRGNGTFKWDNGSFYVGH 240

Query: 241 WSKDPVEQSGTFYPSGSSECEVVLDPQVVYSEYFNECILSTAERILIYPSQKMIS-WSGL 300
           WSKDP E +GT+YPSG +E  +  DP+ +++   +E  + + ER+   PSQK +S W   
Sbjct: 241 WSKDPEEMNGTYYPSG-NEGNLEWDPKDLFNN-LSEYTICSGERVPTLPSQKKLSVW--- 300

Query: 301 DLDSPQKQTNSKKGYDGNRRLRRTSVDGKV---CNSSFESSHDSSSDLSYRNGTEGGGTP 360
                    NS K  +   + RRTSVDG+V    + +FE  +   +++      EGG   
Sbjct: 301 ---------NSSKRIE---KPRRTSVDGRVSVGVDRAFEKMNMWGNEIG-----EGGADM 360

Query: 361 -----------PPQGMESRGKRQSNLRFQPA-KRQGVTISKGHKNYELMLNLQLGIRHSV 420
                        +G++S       ++   A ++QG TISKGH+NYELMLNLQLGIRHSV
Sbjct: 361 RKELDAELMRLDAEGLQSLKSSPVPMKLPKAGRKQGETISKGHRNYELMLNLQLGIRHSV 420

Query: 421 GRPAPATSLDLKNSAFDPKEKVWTKFPPEGSKYTPPHQSHDFRWKDYCPVVFRTLRKLFK 480
           GR APA SLDLK SAFDPK+K+W +FP EG+KYTPPHQS +F+WKDYCP+VFR+LRKLFK
Sbjct: 421 GRQAPAASLDLKPSAFDPKDKIWRRFPREGTKYTPPHQSTEFKWKDYCPLVFRSLRKLFK 480

Query: 481 VDPADYMLSICGNDALRELSSPGKSGSFFYLTHDDRYMIKTMKKAEVKVLLRMLPAYYKH 540
           VDPADYMLSICGNDALRELSSPGKSGSFFYLT+DDRYMIKTMKK+E KVLL ML AYY H
Sbjct: 481 VDPADYMLSICGNDALRELSSPGKSGSFFYLTNDDRYMIKTMKKSETKVLLGMLAAYYNH 540

Query: 541 VRAFENTLVTKFYGLHCVRLTGTTQKKVRFVIMGNLFCSEYNIQRRFDLKGSSHGRTTDK 600
           VRAFEN+LV +F+GLHCV+L G TQKKVRFVIMGNLFCS+Y++ RRFDLKGSS GRTTDK
Sbjct: 541 VRAFENSLVIRFFGLHCVKLNGPTQKKVRFVIMGNLFCSKYSVHRRFDLKGSSLGRTTDK 600

Query: 601 PESEIDATTTLKDLDLNYIFRLRKIWFQEFCRQVFQNLDRDCDFLEQERIMDYSLLVGLH 660
           PESEID+ T LKDLDLN+IFRL+K W+QEF RQ+    D+DC+FLEQERIMDYSLLVG+H
Sbjct: 601 PESEIDSNTILKDLDLNFIFRLQKAWYQEFIRQI----DKDCEFLEQERIMDYSLLVGIH 660

Query: 661 FQETSFREVGTPDSNHSGFCTPI----------------DYFL---TMWASIRLGINMPA 720
           F+E S      P    SG  TPI                D  L   + WASIRLG NMPA
Sbjct: 661 FREASVAGELIP----SGARTPIGESEEESGPRLSRAEVDELLSDPSRWASIRLGTNMPA 720

Query: 721 RAERTVRRGEPEAQLVGEPTGELYDVIIFFGIIDILQDYDISKKLEHAYKSFQYDPSSIS 753
           RAERT+R+ + E QLVGEPTGE Y+V++ FGIIDILQDYDISKKLEHAYKS QYDPSSIS
Sbjct: 721 RAERTMRKNDSELQLVGEPTGEFYEVVMIFGIIDILQDYDISKKLEHAYKSIQYDPSSIS 771

BLAST of MS004276 vs. ExPASy Swiss-Prot
Match: Q56YP2 (Phosphatidylinositol 4-phosphate 5-kinase 1 OS=Arabidopsis thaliana OX=3702 GN=PIP5K1 PE=1 SV=1)

HSP 1 Score: 759.6 bits (1960), Expect = 3.3e-218
Identity = 399/740 (53.92%), Postives = 498/740 (67.30%), Query Frame = 0

Query: 45  PGELHHAEKAIPNGDFYTGQWMDNMPHGHGKYLWTDGCMYVGEWYKGKTLGKGKFSWPSG 104
           P  L   EK +PNGD Y G +    PHG GKYLW DGCMY G+W +GK  GKGKFSWPSG
Sbjct: 65  PTPLVDVEKPLPNGDLYIGSFSGGFPHGSGKYLWKDGCMYEGDWKRGKASGKGKFSWPSG 124

Query: 105 ATYEGDFKSGYMDGKGTYTGSAGDTYRGCWVMNLKHGQGTQNYANGDYYEGEWRRGFQDG 164
           ATYEG+FKSG M+G GT+TG+ GDTYRG WV + KHG G + YANGD+YEG WRR  QDG
Sbjct: 125 ATYEGEFKSGRMEGFGTFTGADGDTYRGTWVADRKHGHGQKRYANGDFYEGTWRRNLQDG 184

Query: 165 QGRYQWKNENHYIGQWKNGKINGNGTMIWSNGNRYDGCWEDGLPKGNGTFRWADGSCFVG 224
           +GRY W+N N Y G+W++G I+G G ++W NGNRY+G WE+G+PKGNG F W+DGS  VG
Sbjct: 185 RGRYVWRNGNQYTGEWRSGVISGKGLLVWPNGNRYEGLWENGIPKGNGVFTWSDGSSCVG 244

Query: 225 VWSKDPVEQSGTFYPSGSSECEVVLDPQVVYSEYFNECILSTAERILIYPSQKMISWSGL 284
            W++  + +S                       +FN       +  LI  ++K  S    
Sbjct: 245 AWNESNIMRS-----------------------FFN----GVEKNDLIVGNRKRSS---- 304

Query: 285 DLDSPQKQTNSKKGYDGNRRLRRTSVDGKVCNSSFESSHDSSSDLSYRNGTEGGGTPPPQ 344
            +DS       +K +    R+     DG+  + + +   +  + + YR+           
Sbjct: 305 -VDSGAGSLGGEKVFP---RICIWESDGEAGDITCDIIDNVEASMIYRDRI--------- 364

Query: 345 GMESRGKRQSNLRFQP------AKRQGVTISKGHKNYELMLNLQLGIRHSVGRPAPATSL 404
            ++  G RQ   +  P      AK+ G TISKGHK Y+LMLNLQLGIR+SVG+ A     
Sbjct: 365 SVDRDGFRQ--FKKNPCWFNGEAKKPGQTISKGHKKYDLMLNLQLGIRYSVGKHASIVR- 424

Query: 405 DLKNSAFDPKEKVWTKFPPEGSKYTPPHQSHDFRWKDYCPVVFRTLRKLFKVDPADYMLS 464
           DLK + FDPKEK WT+FPPEG+K TPPHQS DFRWKDYCP+VFR LR+LF+VDPA YML+
Sbjct: 425 DLKQTDFDPKEKFWTRFPPEGTKTTPPHQSVDFRWKDYCPLVFRRLRELFQVDPAKYMLA 484

Query: 465 ICGNDALRELSSPGKSGSFFYLTHDDRYMIKTMKKAEVKVLLRMLPAYYKHVRAFENTLV 524
           ICGNDALRELSSPGKSGSFFYLT DDR+MIKT+KK+EVKVLLRMLP+YYKHV  +EN+LV
Sbjct: 485 ICGNDALRELSSPGKSGSFFYLTQDDRFMIKTVKKSEVKVLLRMLPSYYKHVCQYENSLV 544

Query: 525 TKFYGLHCVRLTGTTQKKVRFVIMGNLFCSEYNIQRRFDLKGSSHGRTTDKPESEIDATT 584
           T+FYG+HCV+  G   +K RF++MGNLFCSEY IQRRFDLKGSSHGR+T KPE EID TT
Sbjct: 545 TRFYGVHCVKPVG--GQKTRFIVMGNLFCSEYRIQRRFDLKGSSHGRSTAKPEGEIDETT 604

Query: 585 TLKDLDLNYIFRLRKIWFQEFCRQVFQNLDRDCDFLEQERIMDYSLLVGLHFQETSFRE- 644
           TLKDLDLN+ FRL++ W+QE  +Q+     RDC+FLE ERIMDYSLLVG+HF++ +  E 
Sbjct: 605 TLKDLDLNFSFRLQRNWYQELMKQI----KRDCEFLEAERIMDYSLLVGVHFRDDNTGEK 664

Query: 645 ---------VGTPDSNHS-----------GFCTPIDYFLT-MWASIRLGINMPARAERTV 704
                     G  DS  +                +D  L     SIRLG NMPA+AER  
Sbjct: 665 MGLSPFVLRSGRIDSYQNEKFMRGCRFLEAELQDMDRILAGRKPSIRLGANMPAKAERMA 724

Query: 705 RRGEPEAQLVGEPT----GELYDVIIFFGIIDILQDYDISKKLEHAYKSFQYDPSSISAV 753
           RR + +    G  +    GE+Y+V+++FG+IDILQDYDI+KK+EHAYKS Q DP+SISAV
Sbjct: 725 RRSDFDQYSSGGASYPSHGEMYEVVLYFGVIDILQDYDITKKIEHAYKSLQADPASISAV 751

BLAST of MS004276 vs. ExPASy Swiss-Prot
Match: Q8L796 (Phosphatidylinositol 4-phosphate 5-kinase 2 OS=Arabidopsis thaliana OX=3702 GN=PIP5K2 PE=1 SV=2)

HSP 1 Score: 745.7 bits (1924), Expect = 5.0e-214
Identity = 398/733 (54.30%), Postives = 483/733 (65.89%), Query Frame = 0

Query: 52  EKAIPNGDFYTGQWMDNMPHGHGKYLWTDGCMYVGEWYKGKTLGKGKFSWPSGATYEGDF 111
           EK +PNGD Y G +    P+G GKYLW DGCMY GEW +GK  GKGKFSWPSGATYEG+F
Sbjct: 73  EKPLPNGDLYMGTFSGGFPNGSGKYLWKDGCMYEGEWKRGKASGKGKFSWPSGATYEGEF 132

Query: 112 KSGYMDGKGTYTGSAGDTYRGCWVMNLKHGQGTQNYANGDYYEGEWRRGFQDGQGRYQWK 171
           KSG M+G GT+ G  GDTYRG WV + K G G + YANGDYYEG WRR  QDG+GRY W 
Sbjct: 133 KSGRMEGSGTFVGVDGDTYRGSWVADRKQGHGQKRYANGDYYEGTWRRNLQDGRGRYVWM 192

Query: 172 NENHYIGQWKNGKINGNGTMIWSNGNRYDGCWEDGLPKGNGTFRWADGSCFVGVWSKDPV 231
           N N Y G+W+NG I G G + W NGNRY+G WE+G+PKG+G F WADGS ++G W++   
Sbjct: 193 NGNQYTGEWRNGVICGKGVLAWPNGNRYEGQWENGVPKGSGVFTWADGSSWIGSWNESSN 252

Query: 232 EQSGTFYPSGSSECEVVLDPQVVYSEYFNECILSTAERILIYPSQKMISWSGLDLDSPQK 291
                F   G  +               NE I++T +R                +DS   
Sbjct: 253 LMRNFF--DGIEK---------------NELIVATRKR--------------SSVDSGAG 312

Query: 292 QTNSKKGYDGNRRLRRTSVDGKVCNSSFESSHDSSSDLSYRNGTEGGGTPPPQGMESRGK 351
               +K +    R+     DG+  + + +   +  + + YR+            ++  G 
Sbjct: 313 SLTGEKIFP---RICIWESDGEAGDITCDIVDNVEASVIYRDRI---------SIDKDGF 372

Query: 352 RQSNLRFQP------AKRQGVTISKGHKNYELMLNLQLGIRHSVGRPAPATSLDLKNSAF 411
           RQ   R  P      AK+ G TISKGHK Y+LMLNLQ GIR+SVG+ A     DLK S F
Sbjct: 373 RQ--FRKNPCCFSGEAKKPGETISKGHKKYDLMLNLQHGIRYSVGKHASVVR-DLKQSDF 432

Query: 412 DPKEKVWTKFPPEGSKYTPPHQSHDFRWKDYCPVVFRTLRKLFKVDPADYMLSICGNDAL 471
           DP EK WT+FPPEGSK TPPH S DFRWKDYCP+VFR LR+LF VDPADYML+ICGNDAL
Sbjct: 433 DPSEKFWTRFPPEGSKTTPPHLSVDFRWKDYCPLVFRRLRELFTVDPADYMLAICGNDAL 492

Query: 472 RELSSPGKSGSFFYLTHDDRYMIKTMKKAEVKVLLRMLPAYYKHVRAFENTLVTKFYGLH 531
           RELSSPGKSGSFFYLT DDR+MIKT+KK+EVKVLLRMLP+YYKHV  +ENTLVT+FYG+H
Sbjct: 493 RELSSPGKSGSFFYLTQDDRFMIKTVKKSEVKVLLRMLPSYYKHVCQYENTLVTRFYGVH 552

Query: 532 CVRLTGTTQKKVRFVIMGNLFCSEYNIQRRFDLKGSSHGRTTDKPESEIDATTTLKDLDL 591
           C++  G   +K RF++MGNLFCSEY IQRRFDLKGSSHGR T KPE EID TTTLKDLDL
Sbjct: 553 CIKPVG--GQKTRFIVMGNLFCSEYRIQRRFDLKGSSHGRYTSKPEGEIDETTTLKDLDL 612

Query: 592 NYIFRLRKIWFQEFCRQVFQNLDRDCDFLEQERIMDYSLLVGLHFQETSFRE-------- 651
           N+ FRL++ W+QE   Q+     RDC+FLE ERIMDYSLLVG+HF++ +  +        
Sbjct: 613 NFAFRLQRNWYQELMTQI----KRDCEFLEAERIMDYSLLVGVHFRDDNTGDKMGLSPFV 672

Query: 652 --VGTPDSNHS-----------GFCTPIDYFLT-MWASIRLGINMPARAERTVRRGEPEA 711
              G  +S  S                +D  L      IRLG NMPARAER  RR + + 
Sbjct: 673 LRSGKIESYQSEKFMRGCRFLEAELQDMDRILAGRKPLIRLGANMPARAERMARRSDYDQ 732

Query: 712 QLVG----EPTGELYDVIIFFGIIDILQDYDISKKLEHAYKSFQYDPSSISAVDPKQYSK 753
              G    +  GE+Y+V+++FGIIDILQDYDISKK+EHAYKS Q DP+SISAVDPK YS+
Sbjct: 733 YSSGGTNYQSHGEVYEVVLYFGIIDILQDYDISKKIEHAYKSLQADPASISAVDPKLYSR 753

BLAST of MS004276 vs. ExPASy TrEMBL
Match: A0A6J1CBY7 (Phosphatidylinositol 4-phosphate 5-kinase OS=Momordica charantia OX=3673 GN=LOC111010166 PE=4 SV=1)

HSP 1 Score: 1527.3 bits (3953), Expect = 0.0e+00
Identity = 739/766 (96.48%), Postives = 740/766 (96.61%), Query Frame = 0

Query: 1   MYKEYAGFVKAWEATVRKTQSSAKKRANSIFAAMSVAPADDEPGPGELHHAEKAIPNGDF 60
           MYKEYAGFVKAWEATVRKTQSSAKKRANSIFAAMSVAPADDEPGPGELHHAEKAIPNGDF
Sbjct: 1   MYKEYAGFVKAWEATVRKTQSSAKKRANSIFAAMSVAPADDEPGPGELHHAEKAIPNGDF 60

Query: 61  YTGQWMDNMPHGHGKYLWTDGCMYVGEWYKGKTLGKGKFSWPSGATYEGDFKSGYMDGKG 120
           YTGQWMDNMPHGHGKYLWTDGCMYVGEWYKGKTLGKGKFSWPSGATYEGDFKSGYMDGKG
Sbjct: 61  YTGQWMDNMPHGHGKYLWTDGCMYVGEWYKGKTLGKGKFSWPSGATYEGDFKSGYMDGKG 120

Query: 121 TYTGSAGDTYRGCWVMNLKHGQGTQNYANGDYYEGEWRRGFQDGQGRYQWKNENHYIGQW 180
           TYTGSAGDTYRGCWVMNLKHGQGTQNYANGDYYEGEWRRGFQDGQGRYQWKNENHYIGQW
Sbjct: 121 TYTGSAGDTYRGCWVMNLKHGQGTQNYANGDYYEGEWRRGFQDGQGRYQWKNENHYIGQW 180

Query: 181 KNGKINGNGTMIWSNGNRYDGCWEDGLPKGNGTFRWADGSCFVGVWSKDPVEQSGTFYPS 240
           KNGKINGNGTMIWSNGNRYDGCWEDGLPKGNGTFRWADGSCFVGVWSKDPVEQSGTFYPS
Sbjct: 181 KNGKINGNGTMIWSNGNRYDGCWEDGLPKGNGTFRWADGSCFVGVWSKDPVEQSGTFYPS 240

Query: 241 GSSECEVVLDPQVVYSEYFNECILSTAERILIYPSQKMISWSGLDLDSPQKQTNSKKGYD 300
           GSSECEVVLDPQVVYSEYFNECILSTAERILIYPSQKMISWSGLDLDSPQKQTNSKKGYD
Sbjct: 241 GSSECEVVLDPQVVYSEYFNECILSTAERILIYPSQKMISWSGLDLDSPQKQTNSKKGYD 300

Query: 301 GNRRLRRTSVDGKVCNSSFESSHDSSSDLSYRNGTEGGGTPPPQGMESRGKRQSNLRFQP 360
           GNRRLRRTSVDGKVCNSSFESSHDSSSDLSYRNGTEGGGTPPPQGMESRGKRQSNLRFQP
Sbjct: 301 GNRRLRRTSVDGKVCNSSFESSHDSSSDLSYRNGTEGGGTPPPQGMESRGKRQSNLRFQP 360

Query: 361 AKRQGVTISKGHKNYELMLNLQLGIRHSVGRPAPATSLDLKNSAFDPKEKVWTKFPPEGS 420
           AKRQGVTISKGHKNYELMLNLQLGIRHSVGRPAPATSLDLKNSAFDPKEKVWTKFPPEGS
Sbjct: 361 AKRQGVTISKGHKNYELMLNLQLGIRHSVGRPAPATSLDLKNSAFDPKEKVWTKFPPEGS 420

Query: 421 KYTPPHQSHDFRWKDYCPVVFRTLRKLFKVDPADYMLSICGNDALRELSSPGKSGSFFYL 480
           KYTPPHQSHDFRWKDYCPVVFRTLRKLFKVDPADYMLSICGNDALRELSSPGKSGSFFYL
Sbjct: 421 KYTPPHQSHDFRWKDYCPVVFRTLRKLFKVDPADYMLSICGNDALRELSSPGKSGSFFYL 480

Query: 481 THDDRYMIKTMKKAEVKVLLRMLPAYYKHVRAFENTLVTKFYGLHCVRLTGTTQKKVRFV 540
           THDDRYMIKTMKKAEVKVLLRMLPAYYKHVRAFENTLVTKFYGLHCVRLTGTTQKKVRFV
Sbjct: 481 THDDRYMIKTMKKAEVKVLLRMLPAYYKHVRAFENTLVTKFYGLHCVRLTGTTQKKVRFV 540

Query: 541 IMGNLFCSEYNIQRRFDLKGSSHGRTTDKPESEIDATTTLKDLDLNYIFRLRKIWFQEFC 600
           IMGNLFCSEYNIQRRFDLKGSSHGRTTDKPESEIDATTTLKDLDLNYIFRLRKIWFQEFC
Sbjct: 541 IMGNLFCSEYNIQRRFDLKGSSHGRTTDKPESEIDATTTLKDLDLNYIFRLRKIWFQEFC 600

Query: 601 RQVFQNLDRDCDFLEQERIMDYSLLVGLHFQETSFREVGTPDSNHSGFCTP--------- 660
           RQV    DRDCDFLEQERIMDYSLLVGLHFQETSFREVGTPDSNHSG   P         
Sbjct: 601 RQV----DRDCDFLEQERIMDYSLLVGLHFQETSFREVGTPDSNHSGSIDPENEADGQSG 660

Query: 661 -----IDYFLTMWASIRLGINMPARAERTVRRGEPEAQLVGEPTGELYDVIIFFGIIDIL 720
                +    T WASIRLGINMPARAERTVRRGEPEAQLVGEPTGELYDVIIFFGIIDIL
Sbjct: 661 ADKEELAGDSTRWASIRLGINMPARAERTVRRGEPEAQLVGEPTGELYDVIIFFGIIDIL 720

Query: 721 QDYDISKKLEHAYKSFQYDPSSISAVDPKQYSKRFRDFVFRIFLED 753
           QDYDISKKLEHAYKSFQYDPSSISAVDPKQYSKRFRDFVFRIFLED
Sbjct: 721 QDYDISKKLEHAYKSFQYDPSSISAVDPKQYSKRFRDFVFRIFLED 762

BLAST of MS004276 vs. ExPASy TrEMBL
Match: A0A0A0KV49 (Phosphatidylinositol 4-phosphate 5-kinase OS=Cucumis sativus OX=3659 GN=Csa_5G623850 PE=4 SV=1)

HSP 1 Score: 1392.1 bits (3602), Expect = 0.0e+00
Identity = 673/764 (88.09%), Postives = 705/764 (92.28%), Query Frame = 0

Query: 1   MYKEYAGFVKAWEATVRKTQSSAKKRANSIFAAMSVAPADDEPGPGELHHAEKAIPNGDF 60
           MYKEYAG VKAWEATVRKTQ SAKKRANSIFAAMSVAPADDEPGPGELHHAEKAIPNGDF
Sbjct: 1   MYKEYAGIVKAWEATVRKTQHSAKKRANSIFAAMSVAPADDEPGPGELHHAEKAIPNGDF 60

Query: 61  YTGQWMDNMPHGHGKYLWTDGCMYVGEWYKGKTLGKGKFSWPSGATYEGDFKSGYMDGKG 120
           YTGQWMDNMPHGHGKYLWTDGCMYVGEWYKGKTLGKGKFSWPSGATYEGDFKSGYMDGKG
Sbjct: 61  YTGQWMDNMPHGHGKYLWTDGCMYVGEWYKGKTLGKGKFSWPSGATYEGDFKSGYMDGKG 120

Query: 121 TYTGSAGDTYRGCWVMNLKHGQGTQNYANGDYYEGEWRRGFQDGQGRYQWKNENHYIGQW 180
           TYTGS+GDTYRGCWVMNLKHGQGTQNYANGDYYEGEWRRGFQDGQGRYQWKNENHYIGQW
Sbjct: 121 TYTGSSGDTYRGCWVMNLKHGQGTQNYANGDYYEGEWRRGFQDGQGRYQWKNENHYIGQW 180

Query: 181 KNGKINGNGTMIWSNGNRYDGCWEDGLPKGNGTFRWADGSCFVGVWSKDPVEQSGTFYPS 240
           KNGKINGNGTMIW+NGNRYDGCWEDGLPKGNGTFRWADGSC+VGVWSKDP EQ+GTFYPS
Sbjct: 181 KNGKINGNGTMIWNNGNRYDGCWEDGLPKGNGTFRWADGSCYVGVWSKDPTEQTGTFYPS 240

Query: 241 GSSECEVVLDPQVVYSEYFNECILSTAERILIYPSQKMISWSGLDLDSPQKQTNSKKGYD 300
            SSE E+  DPQ +++EY N+ +L + ERILIYPSQKMISWSGLDLDSPQKQT SKKG D
Sbjct: 241 DSSETEMSSDPQEIFAEYLNDFVLCSGERILIYPSQKMISWSGLDLDSPQKQTISKKGND 300

Query: 301 GNRRLRRTSVDGKVCNSSFESSHDSSSDLSYRNGTEGGGTPPPQGMESRGKRQSNLRFQP 360
           GNRRLRRTSVDGKV NSSFES +D S++ S RNG    GT   Q ++S+GKRQSNLRFQP
Sbjct: 301 GNRRLRRTSVDGKVSNSSFESLNDGSNEASSRNGI---GTHQLQDLDSKGKRQSNLRFQP 360

Query: 361 AKRQGVTISKGHKNYELMLNLQLGIRHSVGRPAPATSLDLKNSAFDPKEKVWTKFPPEGS 420
           AKRQG+TISKGHKNYELMLNLQLGIRHSVGRPAPATSLDLK+SAFDPKEKVWTKFPPEGS
Sbjct: 361 AKRQGITISKGHKNYELMLNLQLGIRHSVGRPAPATSLDLKSSAFDPKEKVWTKFPPEGS 420

Query: 421 KYTPPHQSHDFRWKDYCPVVFRTLRKLFKVDPADYMLSICGNDALRELSSPGKSGSFFYL 480
           KYTPPHQS DFRWKDYCPVVFRTLRKLFKVDPADYMLSICGNDALRELSSPGKSGSFFYL
Sbjct: 421 KYTPPHQSSDFRWKDYCPVVFRTLRKLFKVDPADYMLSICGNDALRELSSPGKSGSFFYL 480

Query: 481 THDDRYMIKTMKKAEVKVLLRMLPAYYKHVRAFENTLVTKFYGLHCVRLTGTTQKKVRFV 540
           THDDRYMIKTM+KAEVKVLLRMLPAYYKHVR+FENTLVTKFYGLHCVRLTGT+QKKVRFV
Sbjct: 481 THDDRYMIKTMRKAEVKVLLRMLPAYYKHVRSFENTLVTKFYGLHCVRLTGTSQKKVRFV 540

Query: 541 IMGNLFCSEYNIQRRFDLKGSSHGRTTDKPESEIDATTTLKDLDLNYIFRLRKIWFQEFC 600
           IMGNLFCSEY I RRFDLKGSSHGR TDKPE+EID TTTLKDLDLNYIFRL+KIWFQEFC
Sbjct: 541 IMGNLFCSEYTIHRRFDLKGSSHGRITDKPEAEIDGTTTLKDLDLNYIFRLQKIWFQEFC 600

Query: 601 RQVFQNLDRDCDFLEQERIMDYSLLVGLHFQETSFREVGTPDSNHSGFCTPIDYFL---- 660
           RQV    D+DCDFLEQERIMDYSLLVGLHFQETS+REV TP S+HSG  +  DY      
Sbjct: 601 RQV----DKDCDFLEQERIMDYSLLVGLHFQETSYREVSTPGSHHSG--SNPDYEADGQL 660

Query: 661 --------TMWASIRLGINMPARAERTVRRGEPEAQLVGEPTGELYDVIIFFGIIDILQD 720
                     WASI LGIN+PARAERTVR+GEPEAQLVGEPTGELYDVIIFFGIIDILQD
Sbjct: 661 RADKEQDSARWASISLGINLPARAERTVRQGEPEAQLVGEPTGELYDVIIFFGIIDILQD 720

Query: 721 YDISKKLEHAYKSFQYDPSSISAVDPKQYSKRFRDFVFRIFLED 753
           YDISKKLEHAYKSFQYDPSSISAVDPKQYSKRFRDF+FRIF+ED
Sbjct: 721 YDISKKLEHAYKSFQYDPSSISAVDPKQYSKRFRDFIFRIFVED 755

BLAST of MS004276 vs. ExPASy TrEMBL
Match: A0A5A7SUC1 (Phosphatidylinositol 4-phosphate 5-kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold65G007860 PE=4 SV=1)

HSP 1 Score: 1383.6 bits (3580), Expect = 0.0e+00
Identity = 672/786 (85.50%), Postives = 705/786 (89.69%), Query Frame = 0

Query: 1   MYKEYAGFVKAWEATVRKTQSSAKKRANSIFAAMSVAPADDEPGPGELHHAEKAIPNGDF 60
           MYKEYAG VKAWEATVRKTQ+SAKKRANSIFAAMSVAPADDEPGPGELHHAEKAIPNGDF
Sbjct: 1   MYKEYAGIVKAWEATVRKTQNSAKKRANSIFAAMSVAPADDEPGPGELHHAEKAIPNGDF 60

Query: 61  YTGQWMDNMPHGHGKYLWTDGCMYVGEWYKGKTLGKGKFSWPSGATYEGDFKSGYMDGKG 120
           YTGQWMD+MPHGHGKYLWTDGCMYVGEWYKGKTLGKGKFSWPSGATYEGDFKSGYMDGKG
Sbjct: 61  YTGQWMDHMPHGHGKYLWTDGCMYVGEWYKGKTLGKGKFSWPSGATYEGDFKSGYMDGKG 120

Query: 121 TYTGSAGDTYRGCWVMNLKHGQGTQNYANGDYYEGEWRRGFQDGQGRYQWKNENHYIGQW 180
           TYTGS+GDTYRGCWVMNLKHGQGTQNYANGDYY+GEWRRGFQDGQGRYQWKNENHYIGQW
Sbjct: 121 TYTGSSGDTYRGCWVMNLKHGQGTQNYANGDYYDGEWRRGFQDGQGRYQWKNENHYIGQW 180

Query: 181 KNGKINGNGTMIWSNGNRYDGCWEDGLPKGNGTFRWADGSCFVGVWSKDPVEQSGTFYPS 240
           KNGKINGNGTMIW+NGNRYDGCWEDGLPKGNGTFRWADGSC+VGVWSKDP EQ+GTFYPS
Sbjct: 181 KNGKINGNGTMIWNNGNRYDGCWEDGLPKGNGTFRWADGSCYVGVWSKDPTEQTGTFYPS 240

Query: 241 GSSECEVVLDPQVVYSEYFNECILSTAERILIYPSQKMISWSGLDLDSPQKQTNSKKGYD 300
            SSE E+  DPQ +++EY N+ +L + ERILIYPSQKMISWSGLDLDSPQKQT SKKG D
Sbjct: 241 DSSEAEMSSDPQEIFAEYLNDFVLCSGERILIYPSQKMISWSGLDLDSPQKQTISKKGND 300

Query: 301 GNRRLRRTSVDGKVCNSSFESSHDSSSDLSYRNGTEGGGTPPPQGMESRGKRQSNLRFQP 360
           GNRRLRRTSVDGKV NSSFES +D S++ S RNG    GT   Q ++S+GKRQSNLRFQP
Sbjct: 301 GNRRLRRTSVDGKVSNSSFESLNDGSNEASSRNGI---GTHHLQDLDSKGKRQSNLRFQP 360

Query: 361 AKRQGVTISKGHKNYELMLNLQLGIRHSVGRPAPATSLDLKNSAFDPKEKVWTKFPPEGS 420
           AKRQGVTISKGHKNYELMLNLQLGIRHSVGRPAPATSLDLK+SAFDPKEKVWTKFPPEGS
Sbjct: 361 AKRQGVTISKGHKNYELMLNLQLGIRHSVGRPAPATSLDLKSSAFDPKEKVWTKFPPEGS 420

Query: 421 KYTPPHQSHDFRWKDYCPVVFRTLRKLFKVDPADYMLSICGNDALRELSSPGKSGSFFYL 480
           KYTPPHQS DFRWKDYCPVVFRTLRKLFKVDPADYMLSICGNDALRELSSPGKSGSFFYL
Sbjct: 421 KYTPPHQSSDFRWKDYCPVVFRTLRKLFKVDPADYMLSICGNDALRELSSPGKSGSFFYL 480

Query: 481 THDDRYMIKTMKKAEVKVLLRMLPAYYKHVRAFENTLVTKFYGLHCVRLTGTTQKK---- 540
           THDDRYMIKTM+KAEVKVLLRMLPAYYKHVR+FENTLVTKFYGLHCVRLTGT+QKK    
Sbjct: 481 THDDRYMIKTMRKAEVKVLLRMLPAYYKHVRSFENTLVTKFYGLHCVRLTGTSQKKVYKD 540

Query: 541 --------------------VRFVIMGNLFCSEYNIQRRFDLKGSSHGRTTDKPESEIDA 600
                               VRFVIMGNLFCSEY I RRFDLKGSSHGR TDKPE+EID 
Sbjct: 541 TKAVQWLPWILQFTDFVDLQVRFVIMGNLFCSEYTIHRRFDLKGSSHGRITDKPEAEIDG 600

Query: 601 TTTLKDLDLNYIFRLRKIWFQEFCRQVFQNLDRDCDFLEQERIMDYSLLVGLHFQETSFR 660
           TTTLKDLDLNYIFRL+KIWFQEFCRQV    D+DCDFLEQERIMDYSLLVGLHFQETS+R
Sbjct: 601 TTTLKDLDLNYIFRLQKIWFQEFCRQV----DKDCDFLEQERIMDYSLLVGLHFQETSYR 660

Query: 661 EVGTPDSNHSGFCTPID----------YFLTMWASIRLGINMPARAERTVRRGEPEAQLV 720
           E  TP+S+HSGFC   D               WASI LGIN+PARAERTVRR EPEAQLV
Sbjct: 661 EASTPESHHSGFCCLFDSNGQLRANKEQDSARWASISLGINLPARAERTVRRAEPEAQLV 720

Query: 721 GEPTGELYDVIIFFGIIDILQDYDISKKLEHAYKSFQYDPSSISAVDPKQYSKRFRDFVF 753
           GEPTGELYDVIIFFGIIDILQDYDISKKLEHAYKSFQYDPSSISAVDPKQYSKRFRDF+F
Sbjct: 721 GEPTGELYDVIIFFGIIDILQDYDISKKLEHAYKSFQYDPSSISAVDPKQYSKRFRDFIF 779

BLAST of MS004276 vs. ExPASy TrEMBL
Match: A0A5D3CG23 (Phosphatidylinositol 4-phosphate 5-kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold475G003130 PE=4 SV=1)

HSP 1 Score: 1375.5 bits (3559), Expect = 0.0e+00
Identity = 671/788 (85.15%), Postives = 705/788 (89.47%), Query Frame = 0

Query: 1   MYKEYAGFVKAWEATVRKTQSSAKKRANSIFAAMSVAPADDEPGPGELHHAEKAIPNGDF 60
           MYKEYAG VKAWEATVRKTQ+SAKKRANSIFAAMSVAPADDEPGPGELHHAEKAIPNGDF
Sbjct: 1   MYKEYAGIVKAWEATVRKTQNSAKKRANSIFAAMSVAPADDEPGPGELHHAEKAIPNGDF 60

Query: 61  YTGQWMDNMPHGHGKYLWTDGCMYVGEWYKGKTLGKGKFSWPSGATYEGDFKSGYMDGKG 120
           YTGQWMD+MPHGHGKYLWTDGCMYVGEWYKGKTLGKGKFSWPSGATYEGDFKSGYMDGKG
Sbjct: 61  YTGQWMDHMPHGHGKYLWTDGCMYVGEWYKGKTLGKGKFSWPSGATYEGDFKSGYMDGKG 120

Query: 121 TYTGSAGDTYRGCWVMNLKHGQGTQNYANGDYYEGEWRRGFQDGQGRYQWKNENHYIGQW 180
           TYTGS+GDTYRGCWVMNLKHGQGTQNYANGDYY+GEWRRGFQDGQGRYQWKNENHYIGQW
Sbjct: 121 TYTGSSGDTYRGCWVMNLKHGQGTQNYANGDYYDGEWRRGFQDGQGRYQWKNENHYIGQW 180

Query: 181 KNGKINGNGTMIWSNGNRYDGCWEDGLPKGNGTFRWADGSCFVGVWSKDPVEQSGTFYPS 240
           KNGKINGNGTMIW+NGNRYDGCWEDGLPKGNGTFRWADGSC+VGVWSKDP EQ+GTFYPS
Sbjct: 181 KNGKINGNGTMIWNNGNRYDGCWEDGLPKGNGTFRWADGSCYVGVWSKDPTEQTGTFYPS 240

Query: 241 GSSECEVVLDPQVVYSEYFNECILSTAERILIYPSQKMISWSGLDLDSPQKQTNSKKGYD 300
            SSE E+  DPQ +++EY N+ +L + ERILIYPSQKMISWSGLDLDSPQKQT SKKG D
Sbjct: 241 DSSEAEMSSDPQEIFAEYLNDFVLCSGERILIYPSQKMISWSGLDLDSPQKQTISKKGND 300

Query: 301 GNRRLRRTSVDGKVCNSSFESSHDSSSDLSYRNGTEGGGTPPPQGMESRGKRQSNLRFQP 360
           GNRRLRRTSVDGKV NSSFES +D S++ S RNG    GT   Q ++S+GKRQSNLRFQP
Sbjct: 301 GNRRLRRTSVDGKVSNSSFESLNDGSNEASSRNGI---GTHHLQDLDSKGKRQSNLRFQP 360

Query: 361 AKRQGVTISKGHKNYELMLNLQLGIRHSVGRPAPATSLDLKNSAFDPKEKVWTKFPPEGS 420
           AKRQGVTISKGHKNYELMLNLQLGIRHSVGRPAPATSLDLK+SAFDPKEKVWTKFPPEGS
Sbjct: 361 AKRQGVTISKGHKNYELMLNLQLGIRHSVGRPAPATSLDLKSSAFDPKEKVWTKFPPEGS 420

Query: 421 KYTPPHQSHDFRWKDYCPVVFRTLRKLFKVDPADYMLSICGNDALRELSSPGKSGSFFYL 480
           KYTPPHQS DFRWKDYCPVVFRTLRKLFKVDPADYMLSICGNDALRELSSPGKSGSFFYL
Sbjct: 421 KYTPPHQSSDFRWKDYCPVVFRTLRKLFKVDPADYMLSICGNDALRELSSPGKSGSFFYL 480

Query: 481 THDDRYMIKTMKKAEVKVLLRMLPAYYKHVRAFENTLVTKFYGLHCVRLTGTTQKK---- 540
           THDDRYMIKTM+KAEVKVLLRMLPAYYKHVR+FENTLVTKFYGLHCVRLTGT+QKK    
Sbjct: 481 THDDRYMIKTMRKAEVKVLLRMLPAYYKHVRSFENTLVTKFYGLHCVRLTGTSQKKVYKD 540

Query: 541 --------------------VRFVIMGNLFCSEYNIQRRFDLKGSSHGRTTDKPESEIDA 600
                               VRFVIMGNLFCSEY I RRFDLKGSSHGR TDKPE+EID 
Sbjct: 541 TKAVQWLPWILQFTDFVDLQVRFVIMGNLFCSEYTIHRRFDLKGSSHGRITDKPEAEIDG 600

Query: 601 TTTLKDLDLNYIFRLRKIWFQEFCRQVFQNLDRDCDFLEQERIMDYSLLVGLHFQETSFR 660
           TTTLKDLDLNYIFRL+KIWFQEFCRQV    D+DCDFLEQERIMDYSLLVGLHFQETS+R
Sbjct: 601 TTTLKDLDLNYIFRLQKIWFQEFCRQV----DKDCDFLEQERIMDYSLLVGLHFQETSYR 660

Query: 661 EVGTPDSNHSGFCTPIDYFL------------TMWASIRLGINMPARAERTVRRGEPEAQ 720
           E  TP+S+HSG  +  DY                WASI LGIN+PARAERTVRR EPEAQ
Sbjct: 661 EASTPESHHSG--SNPDYEADGQLRANKEQDSARWASISLGINLPARAERTVRRAEPEAQ 720

Query: 721 LVGEPTGELYDVIIFFGIIDILQDYDISKKLEHAYKSFQYDPSSISAVDPKQYSKRFRDF 753
           LVGEPTGELYDVIIFFGIIDILQDYDISKKLEHAYKSFQYDPSSISAVDPKQYSKRFRDF
Sbjct: 721 LVGEPTGELYDVIIFFGIIDILQDYDISKKLEHAYKSFQYDPSSISAVDPKQYSKRFRDF 779

BLAST of MS004276 vs. ExPASy TrEMBL
Match: A0A6J1GY08 (Phosphatidylinositol 4-phosphate 5-kinase OS=Cucurbita moschata OX=3662 GN=LOC111458505 PE=4 SV=1)

HSP 1 Score: 1319.7 bits (3414), Expect = 0.0e+00
Identity = 633/767 (82.53%), Postives = 687/767 (89.57%), Query Frame = 0

Query: 1   MYKEYAGFVKAWEATVRKTQSSAKKRANSIFAAMSVAPADDEPGPGELHHAEKAIPNGDF 60
           MYK+YAG VKAWEATVRKTQ+SAKKRANSIFAAMSVAPADDEPGPGELHH EKAIPNGDF
Sbjct: 1   MYKDYAGIVKAWEATVRKTQNSAKKRANSIFAAMSVAPADDEPGPGELHHTEKAIPNGDF 60

Query: 61  YTGQWMDNMPHGHGKYLWTDGCMYVGEWYKGKTLGKGKFSWPSGATYEGDFKSGYMDGKG 120
           YTGQWMD+ PHGHGKYLWTDGCMYVGEWYKGKT+GKGKFSWPSGATYEGDFKSGYMDGKG
Sbjct: 61  YTGQWMDSTPHGHGKYLWTDGCMYVGEWYKGKTIGKGKFSWPSGATYEGDFKSGYMDGKG 120

Query: 121 TYTGSAGDTYRGCWVMNLKHGQGTQNYANGDYYEGEWRRGFQDGQGRYQWKNENHYIGQW 180
           TYTGS+GDTYRGCWVMNL+HGQGTQNYANGDYYEGEWRRG QDGQGRYQWKNENHY+GQW
Sbjct: 121 TYTGSSGDTYRGCWVMNLRHGQGTQNYANGDYYEGEWRRGCQDGQGRYQWKNENHYVGQW 180

Query: 181 KNGKINGNGTMIWSNGNRYDGCWEDGLPKGNGTFRWADGSCFVGVWSKDPVEQSGTFYPS 240
           +NGK++GNG+M+W++GNRYDGCWE+G PKG+GTFRWADGS +VGVWSKD  E +G FY S
Sbjct: 181 RNGKMSGNGSMVWNDGNRYDGCWEEGFPKGDGTFRWADGSSYVGVWSKDSTELTGNFYTS 240

Query: 241 GSSECEVVLDPQVVYSEYFNECILSTAERILIYPSQKMISWSGLDLDSPQKQTNSKKGYD 300
            +S  E+  +P+ +  EY N+C++ + ERILIYPSQKMISWSGLDLDS  +QT S+KG D
Sbjct: 241 DTSGTEMSSNPRELLGEYLNDCVICSGERILIYPSQKMISWSGLDLDSLPRQTVSRKGSD 300

Query: 301 GNRRLRRTSVDGKVCNSSFESSHDSSSDLSYRNGTEGGGTPPPQGMESRGKRQSNLRFQP 360
           G RRLRRTSVDGK+ N+SFES +DSS++ S RNG    GT     ++S+GKRQ    FQ 
Sbjct: 301 GIRRLRRTSVDGKISNASFESFNDSSNEPSSRNGK---GT-RLLDLDSKGKRQPTCGFQS 360

Query: 361 AKRQGVTISKGHKNYELMLNLQLGIRHSVGRPAPATSLDLKNSAFDPKEKVWTKFPPEGS 420
           A RQGVTISKGHKNYELMLNLQLGIRHSVGRPAPATSLDLK+SAFDPKEKVWTKFPPEGS
Sbjct: 361 ANRQGVTISKGHKNYELMLNLQLGIRHSVGRPAPATSLDLKSSAFDPKEKVWTKFPPEGS 420

Query: 421 KYTPPHQSHDFRWKDYCPVVFRTLRKLFKVDPADYMLSICGNDALRELSSPGKSGSFFYL 480
           K+TPPHQS DFRWKDYCPVVFRTLRKLF+VDPADYMLSICGNDALRELSSPGKSGSFFYL
Sbjct: 421 KHTPPHQSSDFRWKDYCPVVFRTLRKLFEVDPADYMLSICGNDALRELSSPGKSGSFFYL 480

Query: 481 THDDRYMIKTMKKAEVKVLLRMLPAYYKHVRAFENTLVTKFYGLHCVRLTGTTQKKVRFV 540
           THDDRYMIKTM+KAEVKVLLRMLPAYYKHVR+FENTLVTKFYGLHCVR+TGT QKKVRFV
Sbjct: 481 THDDRYMIKTMRKAEVKVLLRMLPAYYKHVRSFENTLVTKFYGLHCVRVTGTAQKKVRFV 540

Query: 541 IMGNLFCSEYNIQRRFDLKGSSHGRTTDKPESEIDATTTLKDLDLNYIFRLRKIWFQEFC 600
           IMGNLFCSEY I RRFDLKGSS GR TDKPE+EID TTTLKDLDLNYIFRL+KIWFQEFC
Sbjct: 541 IMGNLFCSEYTIHRRFDLKGSSLGRITDKPEAEIDGTTTLKDLDLNYIFRLQKIWFQEFC 600

Query: 601 RQVFQNLDRDCDFLEQERIMDYSLLVGLHFQETSFREVGTPDSNHSGFCTPIDYFLT--- 660
           RQV    D+DCDFLEQERIMDYSLLVGLHFQETS++E  TP+S+HSGFC P+DYFL+   
Sbjct: 601 RQV----DKDCDFLEQERIMDYSLLVGLHFQETSYQEASTPESHHSGFCCPVDYFLSTSN 660

Query: 661 ------------MWASIRLGINMPARAERTVRRGEPEAQLVGEPTGELYDVIIFFGIIDI 720
                        WASI LGINMPAR ERTVR GEPE QLVGEPTGEL+DVIIFFGIIDI
Sbjct: 661 CLSRADKEQDSARWASISLGINMPARVERTVRHGEPETQLVGEPTGELHDVIIFFGIIDI 720

Query: 721 LQDYDISKKLEHAYKSFQYDPSSISAVDPKQYSKRFRDFVFRIFLED 753
           LQDYDISKKLEHAYKSFQYDPSSISAVDPKQYSKRFRDF+FRIFLED
Sbjct: 721 LQDYDISKKLEHAYKSFQYDPSSISAVDPKQYSKRFRDFIFRIFLED 759

BLAST of MS004276 vs. TAIR 10
Match: AT3G07960.1 (Phosphatidylinositol-4-phosphate 5-kinase family protein )

HSP 1 Score: 1055.4 bits (2728), Expect = 2.1e-308
Identity = 524/738 (71.00%), Postives = 587/738 (79.54%), Query Frame = 0

Query: 34  MSVAPADDEPG----PGELHHAEKAIPNGDFYTGQWMDNMPHGHGKYLWTDGCMYVGEWY 93
           MSVA ADD        GE +HAEKA+P+GDFYTGQW DN+PHGHGKYLWTDGCMYVG+W+
Sbjct: 1   MSVAHADDADDYSRPTGESYHAEKALPSGDFYTGQWRDNLPHGHGKYLWTDGCMYVGDWH 60

Query: 94  KGKTLGKGKFSWPSGATYEGDFKSGYMDGKGTYTGSAGDTYRGCWVMNLKHGQGTQNYAN 153
           +GKT+GKG+FSWPSGATYEGDFK+GYMDGKGTY  S+GD YRG WVMNL+HGQGT++Y N
Sbjct: 61  RGKTMGKGRFSWPSGATYEGDFKNGYMDGKGTYIDSSGDLYRGSWVMNLRHGQGTKSYVN 120

Query: 154 GDYYEGEWRRGFQDGQGRYQWKNENHYIGQWKNGKINGNGTMIWSNGNRYDGCWEDGLPK 213
           GD Y+GEWRRG QDG GRYQWKNENHYIGQWKNG +NGNGTMIWSNGNRYDG WEDG PK
Sbjct: 121 GDCYDGEWRRGLQDGHGRYQWKNENHYIGQWKNGLMNGNGTMIWSNGNRYDGSWEDGAPK 180

Query: 214 GNGTFRWADGSCFVGVWSKDPVEQSGTFYPSGSSECEVVLDPQVVYSEYFNECILSTAER 273
           GNGTFRW+DGS +VGVWSKDP EQ+GT+YPS SS       PQ V+    +EC++ T +R
Sbjct: 181 GNGTFRWSDGSFYVGVWSKDPKEQNGTYYPSTSSG-NFDWQPQQVFYVDLSECVVCTCQR 240

Query: 274 ILIYPSQKMISWSGLDLDSPQKQTNSKKGYDGNRRLRRTSVDGKVCNSSFESSHDSSSDL 333
           I + PSQKM  W G       +Q++S      + R RR SVDG+V N   E   + S  L
Sbjct: 241 IPVLPSQKMPVWYG-----ASEQSSSGNRTKNSERPRRRSVDGRVSNGEMELRSNGSGYL 300

Query: 334 SYRNGTEGGGTPPPQGMESRGKRQSNLRFQPAKRQGVTISKGHKNYELMLNLQLGIRHSV 393
              +             ES       LR QPAK+QG TISKGHKNYELMLNLQLGIRHSV
Sbjct: 301 QVDDNA-----------ESTRSSLGPLRIQPAKKQGQTISKGHKNYELMLNLQLGIRHSV 360

Query: 394 GRPAPATSLDLKNSAFDPKEKVWTKFPPEGSKYTPPHQSHDFRWKDYCPVVFRTLRKLFK 453
           GRPAPATSLDLK SAFDPKEK+WTKFP EGSKYTPPHQS +F+WKDYCPVVFRTLRKLF 
Sbjct: 361 GRPAPATSLDLKASAFDPKEKLWTKFPSEGSKYTPPHQSCEFKWKDYCPVVFRTLRKLFS 420

Query: 454 VDPADYMLSICGNDALRELSSPGKSGSFFYLTHDDRYMIKTMKKAEVKVLLRMLPAYYKH 513
           VD ADYMLSICGNDALRELSSPGKSGSFFYLT+DDRYMIKTMKKAE KVL+RMLPAYY H
Sbjct: 421 VDAADYMLSICGNDALRELSSPGKSGSFFYLTNDDRYMIKTMKKAETKVLIRMLPAYYNH 480

Query: 514 VRAFENTLVTKFYGLHCVRLTGTTQKKVRFVIMGNLFCSEYNIQRRFDLKGSSHGRTTDK 573
           VRA ENTLVTKF+GLHCV+LTGT QKKVRFVIMGNLFC+ ++I RRFDLKGSSHGR T K
Sbjct: 481 VRACENTLVTKFFGLHCVKLTGTAQKKVRFVIMGNLFCTGHSIHRRFDLKGSSHGRLTTK 540

Query: 574 PESEIDATTTLKDLDLNYIFRLRKIWFQEFCRQVFQNLDRDCDFLEQERIMDYSLLVGLH 633
           PESEID  TTLKDLDLN+ FRL+K WFQEFCRQV    DRDC+FLEQERIMDYSLLVGLH
Sbjct: 541 PESEIDPNTTLKDLDLNFAFRLQKNWFQEFCRQV----DRDCEFLEQERIMDYSLLVGLH 600

Query: 634 FQETSFREVGTPDSNHSGFCTP------------IDYFL---TMWASIRLGINMPARAER 693
           F+E + ++  TP    SG  TP            +D FL   +  ASI+LGINMPAR ER
Sbjct: 601 FREAAIKDSATPT---SGARTPTGNSETRLSRAEMDRFLLDASKLASIKLGINMPARVER 660

Query: 694 TVRRGEPEAQLVGEPTGELYDVIIFFGIIDILQDYDISKKLEHAYKSFQYDPSSISAVDP 753
           T RR + E QLVG+PTGE YDVI++FGIIDILQDYDISKKLEHAYKS QYDP+SISAVDP
Sbjct: 661 TARRSDCENQLVGDPTGEFYDVIVYFGIIDILQDYDISKKLEHAYKSMQYDPTSISAVDP 714

BLAST of MS004276 vs. TAIR 10
Match: AT3G56960.1 (phosphatidyl inositol monophosphate 5 kinase 4 )

HSP 1 Score: 984.2 bits (2543), Expect = 5.9e-287
Identity = 506/807 (62.70%), Postives = 600/807 (74.35%), Query Frame = 0

Query: 1   MYKEYAGFVKAWEATVRKTQSSAKKRANSIFAAMSVAPADDEPGP--------------- 60
           M KE +  +KAWE TVRKTQ  AKKRANSIF  +SVAP  D+                  
Sbjct: 1   MSKEQSCVLKAWEVTVRKTQ-QAKKRANSIFGTVSVAPQTDDDATTTTEENDDETSTNRS 60

Query: 61  --GELHHAEKAIPNGDFYTGQWMDNMPHGHGKYLWTDGCMYVGEWYKGKTLGKGKFSWPS 120
             GEL+HAE+ +PNGD+YTGQW D+ PHGHGKYLWTDGCMY+G+WY GKT+G+GKF WPS
Sbjct: 61  SIGELYHAERILPNGDYYTGQWYDSFPHGHGKYLWTDGCMYIGDWYNGKTMGRGKFGWPS 120

Query: 121 GATYEGDFKSGYMDGKGTYTGSAGDTYRGCWVMNLKHGQGTQNYANGDYYEGEWRRGFQD 180
           GATYEG+FKSGYMDG G YTG +GDTY+G WVMNLKHG G + +ANGD Y+GEWRRG Q+
Sbjct: 121 GATYEGEFKSGYMDGVGLYTGPSGDTYKGQWVMNLKHGHGIKRFANGDVYDGEWRRGLQE 180

Query: 181 GQGRYQWKNENHYIGQWKNGKINGNGTMIWSNGNRYDGCWEDGLPKGNGTFRWADGSCFV 240
            QG+YQW++ ++Y+G+WKN  I G GT IW++GNRYDG W+DG P+GNGTF+WADGS +V
Sbjct: 181 AQGKYQWRDGSYYMGEWKNATICGKGTFIWTDGNRYDGFWDDGFPRGNGTFKWADGSFYV 240

Query: 241 GVWSKDPVEQSGTFYPSGSSECEVVLDPQVVYSEYFNECILSTAERILIYPSQKMIS-WS 300
           G WS DP E +GT+YPSG  +     DP+ V++   +E  + + ER+ + PSQK +S W 
Sbjct: 241 GHWSNDPEEMNGTYYPSG-DDGSPEWDPKDVFTN-LSEYKICSGERVPVLPSQKKLSVW- 300

Query: 301 GLDLDSPQKQTNSKKGYDGNRRLRRTSVDGKV---CNSSFE-------SSHDSSSDLSYR 360
                      NS K  +   + RR SVDG+V    + +FE        S + ++D+   
Sbjct: 301 -----------NSSKRVE---KPRRMSVDGRVSVGVDRAFEKMNMWGTESGEGAADID-- 360

Query: 361 NGTEGGGTPPPQGMESRGKRQSNLRFQPA--------KRQGVTISKGHKNYELMLNLQLG 420
           + T          +E+ G  QS LR  PA        ++QG TISKGH+NYELMLNLQLG
Sbjct: 361 STTRRDLDAEMMRLEAEGFIQS-LRPSPAPMRLPRAGRKQGETISKGHRNYELMLNLQLG 420

Query: 421 IRHSVGRPAPATSLDLKNSAFDPKEKVWTKFPPEGSKYTPPHQSHDFRWKDYCPVVFRTL 480
           IRH+VG+ AP  SLDLK+SAFDPKEKVWT+FPPEG+KYTPPHQS +F+WKDYCP+VFR+L
Sbjct: 421 IRHAVGKQAPVVSLDLKHSAFDPKEKVWTRFPPEGTKYTPPHQSSEFKWKDYCPLVFRSL 480

Query: 481 RKLFKVDPADYMLSICGNDALRELSSPGKSGSFFYLTHDDRYMIKTMKKAEVKVLLRMLP 540
           RKLFKVDPADYMLSICGNDALRELSSPGKSGSFFYLT+DDRYMIKTMKK+E KVLLRML 
Sbjct: 481 RKLFKVDPADYMLSICGNDALRELSSPGKSGSFFYLTNDDRYMIKTMKKSETKVLLRMLA 540

Query: 541 AYYKHVRAFENTLVTKFYGLHCVRLTGTTQKKVRFVIMGNLFCSEYNIQRRFDLKGSSHG 600
           AYY HVRAFENTLV +FYGLHCV+LTG  QKKVRFVIMGNLFCSEY+I RRFDLKGSS G
Sbjct: 541 AYYNHVRAFENTLVIRFYGLHCVKLTGPIQKKVRFVIMGNLFCSEYSIHRRFDLKGSSLG 600

Query: 601 RTTDKPESEIDATTTLKDLDLNYIFRLRKIWFQEFCRQVFQNLDRDCDFLEQERIMDYSL 660
           RTTDKPESEI++ T LKDLDLN+IFRL+K W+QEF RQV    D+DC+FLEQERIMDYSL
Sbjct: 601 RTTDKPESEINSNTILKDLDLNFIFRLQKAWYQEFIRQV----DKDCEFLEQERIMDYSL 660

Query: 661 LVGLHFQETSFREVGTPDSNHSGFCTPIDYF-------------------LTMWASIRLG 720
           LVG+HF+E S      P    SG  TPI  F                    T WASIRLG
Sbjct: 661 LVGIHFREASVAGELIP----SGARTPIGEFEDESAPRLSRADVDQLLSDPTRWASIRLG 720

Query: 721 INMPARAERTVRRGEPEAQLVGEPTGELYDVIIFFGIIDILQDYDISKKLEHAYKSFQYD 753
            NMPARAERT+RR + E QLVGEPTGE Y+V++ FGIIDILQDYDISKKLEHAYKS QYD
Sbjct: 721 GNMPARAERTMRRSDCEFQLVGEPTGEYYEVVMIFGIIDILQDYDISKKLEHAYKSIQYD 778

BLAST of MS004276 vs. TAIR 10
Match: AT2G41210.1 (phosphatidylinositol- 4-phosphate 5-kinase 5 )

HSP 1 Score: 972.6 bits (2513), Expect = 1.8e-283
Identity = 494/802 (61.60%), Postives = 598/802 (74.56%), Query Frame = 0

Query: 1   MYKEYAGFVKAWEATVRKTQSSAKKRANSIFAAMSVAP----------ADDEPGPG---- 60
           M K+ +  +KAWE TVRKTQ  AKKRANS+F  +SVAP           DDE        
Sbjct: 1   MSKDQSYVLKAWEVTVRKTQ-QAKKRANSVFGTVSVAPHTDNDTTTDDNDDETTTNRSSL 60

Query: 61  -ELHHAEKAIPNGDFYTGQWMDNMPHGHGKYLWTDGCMYVGEWYKGKTLGKGKFSWPSGA 120
            E +HAE+ +PNGD+YTGQW D+ PHGHGKYLWTDGCMY+G+WY GKT+G GKF WPSGA
Sbjct: 61  EEFYHAERVLPNGDYYTGQWYDSFPHGHGKYLWTDGCMYIGDWYNGKTMGNGKFGWPSGA 120

Query: 121 TYEGDFKSGYMDGKGTYTGSAGDTYRGCWVMNLKHGQGTQNYANGDYYEGEWRRGFQDGQ 180
           TYEG+FKSGYMDG GTYTG +GD Y+G WVMNLKHG G +++ANGD Y+GEWRRG Q+GQ
Sbjct: 121 TYEGEFKSGYMDGIGTYTGPSGDAYKGQWVMNLKHGHGVKSFANGDAYDGEWRRGLQEGQ 180

Query: 181 GRYQWKNENHYIGQWKNGKINGNGTMIWSNGNRYDGCWEDGLPKGNGTFRWADGSCFVGV 240
           G+YQW + ++YIG+WKNG I G G+ +W+NGNRYDG W++G P+GNGTF+W +GS +VG 
Sbjct: 181 GKYQWSDGSYYIGEWKNGTICGKGSFVWTNGNRYDGFWDEGFPRGNGTFKWDNGSFYVGH 240

Query: 241 WSKDPVEQSGTFYPSGSSECEVVLDPQVVYSEYFNECILSTAERILIYPSQKMIS-WSGL 300
           WSKDP E +GT+YPSG +E  +  DP+ +++   +E  + + ER+   PSQK +S W   
Sbjct: 241 WSKDPEEMNGTYYPSG-NEGNLEWDPKDLFNN-LSEYTICSGERVPTLPSQKKLSVW--- 300

Query: 301 DLDSPQKQTNSKKGYDGNRRLRRTSVDGKV---CNSSFESSHDSSSDLSYRNGTEGGGTP 360
                    NS K  +   + RRTSVDG+V    + +FE  +   +++      EGG   
Sbjct: 301 ---------NSSKRIE---KPRRTSVDGRVSVGVDRAFEKMNMWGNEIG-----EGGADM 360

Query: 361 -----------PPQGMESRGKRQSNLRFQPA-KRQGVTISKGHKNYELMLNLQLGIRHSV 420
                        +G++S       ++   A ++QG TISKGH+NYELMLNLQLGIRHSV
Sbjct: 361 RKELDAELMRLDAEGLQSLKSSPVPMKLPKAGRKQGETISKGHRNYELMLNLQLGIRHSV 420

Query: 421 GRPAPATSLDLKNSAFDPKEKVWTKFPPEGSKYTPPHQSHDFRWKDYCPVVFRTLRKLFK 480
           GR APA SLDLK SAFDPK+K+W +FP EG+KYTPPHQS +F+WKDYCP+VFR+LRKLFK
Sbjct: 421 GRQAPAASLDLKPSAFDPKDKIWRRFPREGTKYTPPHQSTEFKWKDYCPLVFRSLRKLFK 480

Query: 481 VDPADYMLSICGNDALRELSSPGKSGSFFYLTHDDRYMIKTMKKAEVKVLLRMLPAYYKH 540
           VDPADYMLSICGNDALRELSSPGKSGSFFYLT+DDRYMIKTMKK+E KVLL ML AYY H
Sbjct: 481 VDPADYMLSICGNDALRELSSPGKSGSFFYLTNDDRYMIKTMKKSETKVLLGMLAAYYNH 540

Query: 541 VRAFENTLVTKFYGLHCVRLTGTTQKKVRFVIMGNLFCSEYNIQRRFDLKGSSHGRTTDK 600
           VRAFEN+LV +F+GLHCV+L G TQKKVRFVIMGNLFCS+Y++ RRFDLKGSS GRTTDK
Sbjct: 541 VRAFENSLVIRFFGLHCVKLNGPTQKKVRFVIMGNLFCSKYSVHRRFDLKGSSLGRTTDK 600

Query: 601 PESEIDATTTLKDLDLNYIFRLRKIWFQEFCRQVFQNLDRDCDFLEQERIMDYSLLVGLH 660
           PESEID+ T LKDLDLN+IFRL+K W+QEF RQ+    D+DC+FLEQERIMDYSLLVG+H
Sbjct: 601 PESEIDSNTILKDLDLNFIFRLQKAWYQEFIRQI----DKDCEFLEQERIMDYSLLVGIH 660

Query: 661 FQETSFREVGTPDSNHSGFCTPI----------------DYFL---TMWASIRLGINMPA 720
           F+E S      P    SG  TPI                D  L   + WASIRLG NMPA
Sbjct: 661 FREASVAGELIP----SGARTPIGESEEESGPRLSRAEVDELLSDPSRWASIRLGTNMPA 720

Query: 721 RAERTVRRGEPEAQLVGEPTGELYDVIIFFGIIDILQDYDISKKLEHAYKSFQYDPSSIS 753
           RAERT+R+ + E QLVGEPTGE Y+V++ FGIIDILQDYDISKKLEHAYKS QYDPSSIS
Sbjct: 721 RAERTMRKNDSELQLVGEPTGEFYEVVMIFGIIDILQDYDISKKLEHAYKSIQYDPSSIS 771

BLAST of MS004276 vs. TAIR 10
Match: AT1G21980.1 (phosphatidylinositol-4-phosphate 5-kinase 1 )

HSP 1 Score: 759.6 bits (1960), Expect = 2.4e-219
Identity = 399/740 (53.92%), Postives = 498/740 (67.30%), Query Frame = 0

Query: 45  PGELHHAEKAIPNGDFYTGQWMDNMPHGHGKYLWTDGCMYVGEWYKGKTLGKGKFSWPSG 104
           P  L   EK +PNGD Y G +    PHG GKYLW DGCMY G+W +GK  GKGKFSWPSG
Sbjct: 65  PTPLVDVEKPLPNGDLYIGSFSGGFPHGSGKYLWKDGCMYEGDWKRGKASGKGKFSWPSG 124

Query: 105 ATYEGDFKSGYMDGKGTYTGSAGDTYRGCWVMNLKHGQGTQNYANGDYYEGEWRRGFQDG 164
           ATYEG+FKSG M+G GT+TG+ GDTYRG WV + KHG G + YANGD+YEG WRR  QDG
Sbjct: 125 ATYEGEFKSGRMEGFGTFTGADGDTYRGTWVADRKHGHGQKRYANGDFYEGTWRRNLQDG 184

Query: 165 QGRYQWKNENHYIGQWKNGKINGNGTMIWSNGNRYDGCWEDGLPKGNGTFRWADGSCFVG 224
           +GRY W+N N Y G+W++G I+G G ++W NGNRY+G WE+G+PKGNG F W+DGS  VG
Sbjct: 185 RGRYVWRNGNQYTGEWRSGVISGKGLLVWPNGNRYEGLWENGIPKGNGVFTWSDGSSCVG 244

Query: 225 VWSKDPVEQSGTFYPSGSSECEVVLDPQVVYSEYFNECILSTAERILIYPSQKMISWSGL 284
            W++  + +S                       +FN       +  LI  ++K  S    
Sbjct: 245 AWNESNIMRS-----------------------FFN----GVEKNDLIVGNRKRSS---- 304

Query: 285 DLDSPQKQTNSKKGYDGNRRLRRTSVDGKVCNSSFESSHDSSSDLSYRNGTEGGGTPPPQ 344
            +DS       +K +    R+     DG+  + + +   +  + + YR+           
Sbjct: 305 -VDSGAGSLGGEKVFP---RICIWESDGEAGDITCDIIDNVEASMIYRDRI--------- 364

Query: 345 GMESRGKRQSNLRFQP------AKRQGVTISKGHKNYELMLNLQLGIRHSVGRPAPATSL 404
            ++  G RQ   +  P      AK+ G TISKGHK Y+LMLNLQLGIR+SVG+ A     
Sbjct: 365 SVDRDGFRQ--FKKNPCWFNGEAKKPGQTISKGHKKYDLMLNLQLGIRYSVGKHASIVR- 424

Query: 405 DLKNSAFDPKEKVWTKFPPEGSKYTPPHQSHDFRWKDYCPVVFRTLRKLFKVDPADYMLS 464
           DLK + FDPKEK WT+FPPEG+K TPPHQS DFRWKDYCP+VFR LR+LF+VDPA YML+
Sbjct: 425 DLKQTDFDPKEKFWTRFPPEGTKTTPPHQSVDFRWKDYCPLVFRRLRELFQVDPAKYMLA 484

Query: 465 ICGNDALRELSSPGKSGSFFYLTHDDRYMIKTMKKAEVKVLLRMLPAYYKHVRAFENTLV 524
           ICGNDALRELSSPGKSGSFFYLT DDR+MIKT+KK+EVKVLLRMLP+YYKHV  +EN+LV
Sbjct: 485 ICGNDALRELSSPGKSGSFFYLTQDDRFMIKTVKKSEVKVLLRMLPSYYKHVCQYENSLV 544

Query: 525 TKFYGLHCVRLTGTTQKKVRFVIMGNLFCSEYNIQRRFDLKGSSHGRTTDKPESEIDATT 584
           T+FYG+HCV+  G   +K RF++MGNLFCSEY IQRRFDLKGSSHGR+T KPE EID TT
Sbjct: 545 TRFYGVHCVKPVG--GQKTRFIVMGNLFCSEYRIQRRFDLKGSSHGRSTAKPEGEIDETT 604

Query: 585 TLKDLDLNYIFRLRKIWFQEFCRQVFQNLDRDCDFLEQERIMDYSLLVGLHFQETSFRE- 644
           TLKDLDLN+ FRL++ W+QE  +Q+     RDC+FLE ERIMDYSLLVG+HF++ +  E 
Sbjct: 605 TLKDLDLNFSFRLQRNWYQELMKQI----KRDCEFLEAERIMDYSLLVGVHFRDDNTGEK 664

Query: 645 ---------VGTPDSNHS-----------GFCTPIDYFLT-MWASIRLGINMPARAERTV 704
                     G  DS  +                +D  L     SIRLG NMPA+AER  
Sbjct: 665 MGLSPFVLRSGRIDSYQNEKFMRGCRFLEAELQDMDRILAGRKPSIRLGANMPAKAERMA 724

Query: 705 RRGEPEAQLVGEPT----GELYDVIIFFGIIDILQDYDISKKLEHAYKSFQYDPSSISAV 753
           RR + +    G  +    GE+Y+V+++FG+IDILQDYDI+KK+EHAYKS Q DP+SISAV
Sbjct: 725 RRSDFDQYSSGGASYPSHGEMYEVVLYFGVIDILQDYDITKKIEHAYKSLQADPASISAV 751

BLAST of MS004276 vs. TAIR 10
Match: AT1G77740.1 (phosphatidylinositol-4-phosphate 5-kinase 2 )

HSP 1 Score: 745.7 bits (1924), Expect = 3.5e-215
Identity = 398/733 (54.30%), Postives = 483/733 (65.89%), Query Frame = 0

Query: 52  EKAIPNGDFYTGQWMDNMPHGHGKYLWTDGCMYVGEWYKGKTLGKGKFSWPSGATYEGDF 111
           EK +PNGD Y G +    P+G GKYLW DGCMY GEW +GK  GKGKFSWPSGATYEG+F
Sbjct: 73  EKPLPNGDLYMGTFSGGFPNGSGKYLWKDGCMYEGEWKRGKASGKGKFSWPSGATYEGEF 132

Query: 112 KSGYMDGKGTYTGSAGDTYRGCWVMNLKHGQGTQNYANGDYYEGEWRRGFQDGQGRYQWK 171
           KSG M+G GT+ G  GDTYRG WV + K G G + YANGDYYEG WRR  QDG+GRY W 
Sbjct: 133 KSGRMEGSGTFVGVDGDTYRGSWVADRKQGHGQKRYANGDYYEGTWRRNLQDGRGRYVWM 192

Query: 172 NENHYIGQWKNGKINGNGTMIWSNGNRYDGCWEDGLPKGNGTFRWADGSCFVGVWSKDPV 231
           N N Y G+W+NG I G G + W NGNRY+G WE+G+PKG+G F WADGS ++G W++   
Sbjct: 193 NGNQYTGEWRNGVICGKGVLAWPNGNRYEGQWENGVPKGSGVFTWADGSSWIGSWNESSN 252

Query: 232 EQSGTFYPSGSSECEVVLDPQVVYSEYFNECILSTAERILIYPSQKMISWSGLDLDSPQK 291
                F   G  +               NE I++T +R                +DS   
Sbjct: 253 LMRNFF--DGIEK---------------NELIVATRKR--------------SSVDSGAG 312

Query: 292 QTNSKKGYDGNRRLRRTSVDGKVCNSSFESSHDSSSDLSYRNGTEGGGTPPPQGMESRGK 351
               +K +    R+     DG+  + + +   +  + + YR+            ++  G 
Sbjct: 313 SLTGEKIFP---RICIWESDGEAGDITCDIVDNVEASVIYRDRI---------SIDKDGF 372

Query: 352 RQSNLRFQP------AKRQGVTISKGHKNYELMLNLQLGIRHSVGRPAPATSLDLKNSAF 411
           RQ   R  P      AK+ G TISKGHK Y+LMLNLQ GIR+SVG+ A     DLK S F
Sbjct: 373 RQ--FRKNPCCFSGEAKKPGETISKGHKKYDLMLNLQHGIRYSVGKHASVVR-DLKQSDF 432

Query: 412 DPKEKVWTKFPPEGSKYTPPHQSHDFRWKDYCPVVFRTLRKLFKVDPADYMLSICGNDAL 471
           DP EK WT+FPPEGSK TPPH S DFRWKDYCP+VFR LR+LF VDPADYML+ICGNDAL
Sbjct: 433 DPSEKFWTRFPPEGSKTTPPHLSVDFRWKDYCPLVFRRLRELFTVDPADYMLAICGNDAL 492

Query: 472 RELSSPGKSGSFFYLTHDDRYMIKTMKKAEVKVLLRMLPAYYKHVRAFENTLVTKFYGLH 531
           RELSSPGKSGSFFYLT DDR+MIKT+KK+EVKVLLRMLP+YYKHV  +ENTLVT+FYG+H
Sbjct: 493 RELSSPGKSGSFFYLTQDDRFMIKTVKKSEVKVLLRMLPSYYKHVCQYENTLVTRFYGVH 552

Query: 532 CVRLTGTTQKKVRFVIMGNLFCSEYNIQRRFDLKGSSHGRTTDKPESEIDATTTLKDLDL 591
           C++  G   +K RF++MGNLFCSEY IQRRFDLKGSSHGR T KPE EID TTTLKDLDL
Sbjct: 553 CIKPVG--GQKTRFIVMGNLFCSEYRIQRRFDLKGSSHGRYTSKPEGEIDETTTLKDLDL 612

Query: 592 NYIFRLRKIWFQEFCRQVFQNLDRDCDFLEQERIMDYSLLVGLHFQETSFRE-------- 651
           N+ FRL++ W+QE   Q+     RDC+FLE ERIMDYSLLVG+HF++ +  +        
Sbjct: 613 NFAFRLQRNWYQELMTQI----KRDCEFLEAERIMDYSLLVGVHFRDDNTGDKMGLSPFV 672

Query: 652 --VGTPDSNHS-----------GFCTPIDYFLT-MWASIRLGINMPARAERTVRRGEPEA 711
              G  +S  S                +D  L      IRLG NMPARAER  RR + + 
Sbjct: 673 LRSGKIESYQSEKFMRGCRFLEAELQDMDRILAGRKPLIRLGANMPARAERMARRSDYDQ 732

Query: 712 QLVG----EPTGELYDVIIFFGIIDILQDYDISKKLEHAYKSFQYDPSSISAVDPKQYSK 753
              G    +  GE+Y+V+++FGIIDILQDYDISKK+EHAYKS Q DP+SISAVDPK YS+
Sbjct: 733 YSSGGTNYQSHGEVYEVVLYFGIIDILQDYDISKKIEHAYKSLQADPASISAVDPKLYSR 753

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022139196.10.0e+0096.48phosphatidylinositol 4-phosphate 5-kinase 6-like [Momordica charantia][more]
XP_004142482.10.0e+0088.09phosphatidylinositol 4-phosphate 5-kinase 6 [Cucumis sativus] >KGN52322.1 hypoth... [more]
XP_038893257.10.0e+0087.96phosphatidylinositol 4-phosphate 5-kinase 6-like [Benincasa hispida][more]
KAA0034762.10.0e+0085.50phosphatidylinositol 4-phosphate 5-kinase 6 [Cucumis melo var. makuwa][more]
TYK09316.10.0e+0085.15phosphatidylinositol 4-phosphate 5-kinase 6 isoform X2 [Cucumis melo var. makuwa... [more]
Match NameE-valueIdentityDescription
Q9SFB82.9e-30771.00Phosphatidylinositol 4-phosphate 5-kinase 6 OS=Arabidopsis thaliana OX=3702 GN=P... [more]
Q9M1K28.3e-28662.70Phosphatidylinositol 4-phosphate 5-kinase 4 OS=Arabidopsis thaliana OX=3702 GN=P... [more]
Q9SLG92.5e-28261.60Phosphatidylinositol 4-phosphate 5-kinase 5 OS=Arabidopsis thaliana OX=3702 GN=P... [more]
Q56YP23.3e-21853.92Phosphatidylinositol 4-phosphate 5-kinase 1 OS=Arabidopsis thaliana OX=3702 GN=P... [more]
Q8L7965.0e-21454.30Phosphatidylinositol 4-phosphate 5-kinase 2 OS=Arabidopsis thaliana OX=3702 GN=P... [more]
Match NameE-valueIdentityDescription
A0A6J1CBY70.0e+0096.48Phosphatidylinositol 4-phosphate 5-kinase OS=Momordica charantia OX=3673 GN=LOC1... [more]
A0A0A0KV490.0e+0088.09Phosphatidylinositol 4-phosphate 5-kinase OS=Cucumis sativus OX=3659 GN=Csa_5G62... [more]
A0A5A7SUC10.0e+0085.50Phosphatidylinositol 4-phosphate 5-kinase OS=Cucumis melo var. makuwa OX=1194695... [more]
A0A5D3CG230.0e+0085.15Phosphatidylinositol 4-phosphate 5-kinase OS=Cucumis melo var. makuwa OX=1194695... [more]
A0A6J1GY080.0e+0082.53Phosphatidylinositol 4-phosphate 5-kinase OS=Cucurbita moschata OX=3662 GN=LOC11... [more]
Match NameE-valueIdentityDescription
AT3G07960.12.1e-30871.00Phosphatidylinositol-4-phosphate 5-kinase family protein [more]
AT3G56960.15.9e-28762.70phosphatidyl inositol monophosphate 5 kinase 4 [more]
AT2G41210.11.8e-28361.60phosphatidylinositol- 4-phosphate 5-kinase 5 [more]
AT1G21980.12.4e-21953.92phosphatidylinositol-4-phosphate 5-kinase 1 [more]
AT1G77740.13.5e-21554.30phosphatidylinositol-4-phosphate 5-kinase 2 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002498Phosphatidylinositol-4-phosphate 5-kinase, coreSMARTSM00330PIPK_2coord: 401..750
e-value: 1.3E-172
score: 589.4
IPR002498Phosphatidylinositol-4-phosphate 5-kinase, corePFAMPF01504PIP5Kcoord: 462..747
e-value: 7.2E-90
score: 300.9
IPR002498Phosphatidylinositol-4-phosphate 5-kinase, corePROSITEPS51455PIPKcoord: 367..749
score: 67.865318
IPR003409MORN motifSMARTSM00698morncoord: 197..218
e-value: 2.4E-4
score: 30.4
coord: 151..172
e-value: 7.2E-5
score: 32.2
coord: 128..149
e-value: 0.0027
score: 26.9
coord: 174..195
e-value: 1.6E-6
score: 37.7
coord: 82..103
e-value: 0.11
score: 21.4
coord: 105..126
e-value: 0.11
score: 21.2
coord: 59..80
e-value: 1.1E-5
score: 34.9
IPR003409MORN motifPFAMPF02493MORNcoord: 84..105
e-value: 4.5E-4
score: 19.9
coord: 130..151
e-value: 3.4E-4
score: 20.3
coord: 61..83
e-value: 8.0E-8
score: 31.7
coord: 199..220
e-value: 1.1E-5
score: 25.0
coord: 176..198
e-value: 6.6E-8
score: 32.0
coord: 153..172
e-value: 2.9E-5
score: 23.7
coord: 107..129
e-value: 5.6E-4
score: 19.6
IPR027484Phosphatidylinositol-4-phosphate 5-kinase, N-terminalGENE3D3.30.800.10Phosphatidylinositol Phosphate Kinase II Betacoord: 360..544
e-value: 2.4E-68
score: 231.6
IPR017163Phosphatidylinositol-4-phosphate 5-kinase, plantPIRSFPIRSF037274PIP5K_plantcoord: 4..752
e-value: 0.0
score: 1101.7
NoneNo IPR availableGENE3D3.30.810.10coord: 545..749
e-value: 4.1E-44
score: 152.3
NoneNo IPR availableGENE3D2.20.110.10coord: 136..182
e-value: 1.6E-11
score: 45.7
coord: 183..246
e-value: 3.5E-12
score: 47.8
coord: 50..90
e-value: 6.8E-7
score: 30.9
coord: 91..135
e-value: 7.6E-9
score: 37.1
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 311..334
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 347..362
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 283..369
NoneNo IPR availablePANTHERPTHR23086:SF29PHOSPHATIDYLINOSITOL 4-PHOSPHATE 5-KINASE 4coord: 41..752
NoneNo IPR availableCDDcd17302PIPKc_AtPIP5K_likecoord: 374..748
e-value: 0.0
score: 596.194
NoneNo IPR availableSUPERFAMILY82185Histone H3 K4-specific methyltransferase SET7/9 N-terminal domaincoord: 147..242
NoneNo IPR availableSUPERFAMILY82185Histone H3 K4-specific methyltransferase SET7/9 N-terminal domaincoord: 56..172
NoneNo IPR availableSUPERFAMILY56104SAICAR synthase-likecoord: 370..749
IPR023610Phosphatidylinositol-4-phosphate 5-kinasePANTHERPTHR23086PHOSPHATIDYLINOSITOL-4-PHOSPHATE 5-KINASEcoord: 41..752

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS004276.1MS004276.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0046488 phosphatidylinositol metabolic process
molecular_function GO:0016308 1-phosphatidylinositol-4-phosphate 5-kinase activity
molecular_function GO:0005524 ATP binding
molecular_function GO:0016307 phosphatidylinositol phosphate kinase activity