MS004265 (gene) Bitter gourd (TR) v1

Overview
NameMS004265
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionF-box domain-containing protein
Locationscaffold92: 1325141 .. 1328218 (+)
RNA-Seq ExpressionMS004265
SyntenyMS004265
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
GTTTCAGTGCAAGAAATTAAGCAGAGAAAGTGGAAGAGAAAAGGCACTTCATGTTCATCCTCCTCGGCTCGTATAAACAATTTGGACGATGGCTGTTTAATGCATATATTCAGCTTCCTTTCCCCCATACCAGGTACCTTTCAATTTGCTATCTGAAATTCTATTCCTTCAATTTTTGGTCCTATTCCGTTTTGAGGTTTTTGGTTTCCTGTTCAAGTTGATTCATTTCGCTGAAATTGAAGGACTTATCCGTTCAATTTGCAAAACCGGGAGGTTATCTGGAGGAAATTTTGAAACCTTGGGTGAAAAACCATTCGATTATAGGAGAAATCGATAGAGTGGACTTTTGAAATCTTTTGGATACTGCTGGAATTAGATTACCGGAAGAAGAAGAATTGTAATTTCGGGTTTGACATGAGTCGGGAATTATTGTTTACTTGTTCCTGTTAGAAAACCTATTGGATAAAAAGATGGGATGCTTGGTTTCTTTACGTTGGACCCATTGGAGTCTATAATTGGGCCTTTTTGGTTTTCGTTAGGTGTGGTTTAGGAGAATTTTTGGACTTTGGATATGAAGAGGTTGGATACTTTGTTATACGATGGCTGAATGCTGATGCAGTTGTTGTTGCTGTATTGCCCTACCTTCCATCAGCTGGTGTATTGTGCCTTTGTGGTCGAGTTGCGAACCAATTGGGGGCGAGGGATAAGAAAAATTTGGAAACAAAAGTGAAGATGTTGATGAAGTTTAAGATGAAGCTAAATATAATTCTTACTGAAACTGATGCTCTAGAATGCCATTTTCTTCTCTCTAGTTATTTTGCTTAGTCTAGTGAGAAAATTTTGTATCGTTAGTTTCATCGAGTTGTAGTTAAGTGGATGCGGTCCGCTTATTTCTTTCTAATTTTAATTGGTAGTAAGTTTTGTTGCGCACGTTTTTAGAAAAAGGATTCTCCATGGAGTTTCTGCCTTTTCTGTCACTTTTGCCATTGAGTTTGCAGATCGGTATAACACCGCCCTCGTTTGCCACAGATGGCGTTACTTGGCATGTCATCCTCGGCTGTGGCTGCGGGTAGAACGATCTATTAAAGATTTATCTGAGTCTGGAGTTTTCCCAACTATTGAGGCAGCTGTTGCTGCTGCAAGGTAAGTTTTTTAATGTCCATGTTTTTACTTTTCAAGAGAGGAAATAATAAAAGTAGGTTGCTAATAGAGTCTGAAATTGAATGATTTTGTTCTCCATAGGCCTGGTGACACCATACTAATTGCAACAGGTGGAGTTCATTCTGCCTCTAACATTCAAATAACAAAACCACTTTGCCTGGTATGTTCTTTTTTGTTCCCTTTTTTTTTCTGCTTGGTAGTGACATTTTACTCCTCATATATTGTTTAAATGTCATCCATTTTTTTGTAATGAACCAGACTTTCATTGAGAAAACAAAATATGAAAGAGAACGAAGACAATGGGACATATAAAAGACAACCTAAAGACGGGAATATAAGAGGGGACTCCAACTCAAATTAATATGACCTAAAGAATAAGAACAAAACTCCTTAGTAACCGACGCCCAATCTAGCAAGACACTACACCATCTCTGGAGATTTCTCCCAATCCCTAAATGTTCTATTGTTCCTTTCCAACCAAAGACTCCAAATCACTGCAAAAAGTTTAAATGTCGTCAAATTGTCAGAAATATCTGTTTGTTTGTGCATAATTTAGGTCTCCTTTCAAAGAATAAGTGCCTTGTTATGTTATAATGTGCTAGGATTGTTTACTGATACCAAACAGTCTCTTTAAAGGTCACTATATAGAGCTAGAAGCTAGTTAGCTATTTGGGTTCTGGCTATTCAGCTTTAAAGAAACCTTAGCAGAATTTTTTTCTCTTTTCGATTAGAAACCGAATAACTATTCTGTTTTAAATATTGGTGAGACGGTGGCTTCCTTGTCAAGACGTGCCCAGTTTTTGGACTATAAGATCTTTTAACTTCAAAATTTTTAGTACCCTGTATACTTCTTTTGTTCCATTATGTTCCATCATATTAAAGATTTTCACCTGACATTTTTTGCTGAACTTATGAGTTATGACACACGATAACACCTTCATATAGAACCTAAGTCTCACTATTTTCCATCATTTTCTTAACTCAAAGATTGGTGGAGGTGAGCTTCCTGAGGAGACAACGCTTTTCTGCACTCGTGGTTCGGAAAGGTACACGTTCATAACATATAGTGTAATTTGGAATTGTGATCCTAGAGGTGTTGTCTCCATTCTAACATCTTCAAGAGATCATTTATCATCATCCATTTGGACTTGCTATTGTTATTGATGGTTCTCTAATCTAATTCACCATTTACTTCAAGCTGACTTTGCTCGACGAATATATCATGTTATCATTAAAATATAATAATTTAATCTGGTATTTGAACTTTTGAAAGATCTAATTTAGCGTTAAACTTTCAGAAAGTAACCACTTTGATATTTGTGATTATTTTGCCACCTAATGTACCTATGTGGCTCCTATACATGTTCACATAGCACATTAGTACATATTCTCGAAGCCATTTTCTGCATTCATAAAAAGCAAAAGACTAAAATGATCGACTACGATAGCCGTTTTGAAAGGATAAAAATGGATATTTTGAAAATTCAAAGACCAAATCTTTGAACTATTTTATTTTAGGATTTGTAGAGGCAACTCATTATGTAATTTCTATTTCTTCTTAACAGTGCTTTGGAGTTCCTGTGTACCAGTAAACTTTCAAATTTAACAGTGAAGGCAGAACTTGGTTGCTGCTTGCTTCACAGGAAGGGAAGATTAATTATAGATGGATGTGTCCTTCAATGTGAGTCAAACCCTTTGGATTACCTATCATGCCCCATTGTTTGTTCAGCAAATCCCGATAAGTTATTGCCTTCCTCGGTGAAGGGTGGCTATACAGATGGCGTTTCTGTTTCGCAAACCCGCATTGAGGGTGGTGCTAAAGCTGTCCTAACAAGCGAGGACCTGGCATTACAGCACGTTCGAGTTATTTATGCTCGAACGGCTCTGTTCTTTTGGTTTGATGTCGAATACAAGTTG

mRNA sequence

GTTTCAGTGCAAGAAATTAAGCAGAGAAAGTGGAAGAGAAAAGGCACTTCATGTTCATCCTCCTCGGCTCGTATAAACAATTTGGACGATGGCTGTTTAATGCATATATTCAGCTTCCTTTCCCCCATACCAGATCGGTATAACACCGCCCTCGTTTGCCACAGATGGCGTTACTTGGCATGTCATCCTCGGCTGTGGCTGCGGGTAGAACGATCTATTAAAGATTTATCTGAGTCTGGAGTTTTCCCAACTATTGAGGCAGCTGTTGCTGCTGCAAGGCCTGGTGACACCATACTAATTGCAACAGGTGGAGTTCATTCTGCCTCTAACATTCAAATAACAAAACCACTTTGCCTGATTGGTGGAGGTGAGCTTCCTGAGGAGACAACGCTTTTCTGCACTCGTGGTTCGGAAAGTGCTTTGGAGTTCCTGTGTACCAGTAAACTTTCAAATTTAACAGTGAAGGCAGAACTTGGTTGCTGCTTGCTTCACAGGAAGGGAAGATTAATTATAGATGGATGTGTCCTTCAATGTGAGTCAAACCCTTTGGATTACCTATCATGCCCCATTGTTTGTTCAGCAAATCCCGATAAGTTATTGCCTTCCTCGGTGAAGGGTGGCTATACAGATGGCGTTTCTGTTTCGCAAACCCGCATTGAGGGTGGTGCTAAAGCTGTCCTAACAAGCGAGGACCTGGCATTACAGCACGTTCGAGTTATTTATGCTCGAACGGCTCTGTTCTTTTGGTTTGATGTCGAATACAAGTTG

Coding sequence (CDS)

GTTTCAGTGCAAGAAATTAAGCAGAGAAAGTGGAAGAGAAAAGGCACTTCATGTTCATCCTCCTCGGCTCGTATAAACAATTTGGACGATGGCTGTTTAATGCATATATTCAGCTTCCTTTCCCCCATACCAGATCGGTATAACACCGCCCTCGTTTGCCACAGATGGCGTTACTTGGCATGTCATCCTCGGCTGTGGCTGCGGGTAGAACGATCTATTAAAGATTTATCTGAGTCTGGAGTTTTCCCAACTATTGAGGCAGCTGTTGCTGCTGCAAGGCCTGGTGACACCATACTAATTGCAACAGGTGGAGTTCATTCTGCCTCTAACATTCAAATAACAAAACCACTTTGCCTGATTGGTGGAGGTGAGCTTCCTGAGGAGACAACGCTTTTCTGCACTCGTGGTTCGGAAAGTGCTTTGGAGTTCCTGTGTACCAGTAAACTTTCAAATTTAACAGTGAAGGCAGAACTTGGTTGCTGCTTGCTTCACAGGAAGGGAAGATTAATTATAGATGGATGTGTCCTTCAATGTGAGTCAAACCCTTTGGATTACCTATCATGCCCCATTGTTTGTTCAGCAAATCCCGATAAGTTATTGCCTTCCTCGGTGAAGGGTGGCTATACAGATGGCGTTTCTGTTTCGCAAACCCGCATTGAGGGTGGTGCTAAAGCTGTCCTAACAAGCGAGGACCTGGCATTACAGCACGTTCGAGTTATTTATGCTCGAACGGCTCTGTTCTTTTGGTTTGATGTCGAATACAAGTTG

Protein sequence

VSVQEIKQRKWKRKGTSCSSSSARINNLDDGCLMHIFSFLSPIPDRYNTALVCHRWRYLACHPRLWLRVERSIKDLSESGVFPTIEAAVAAARPGDTILIATGGVHSASNIQITKPLCLIGGGELPEETTLFCTRGSESALEFLCTSKLSNLTVKAELGCCLLHRKGRLIIDGCVLQCESNPLDYLSCPIVCSANPDKLLPSSVKGGYTDGVSVSQTRIEGGAKAVLTSEDLALQHVRVIYARTALFFWFDVEYKL
Homology
BLAST of MS004265 vs. NCBI nr
Match: XP_022139176.1 (F-box protein SKIP5 [Momordica charantia])

HSP 1 Score: 518.5 bits (1334), Expect = 3.4e-143
Identity = 255/256 (99.61%), Postives = 255/256 (99.61%), Query Frame = 0

Query: 1   VSVQEIKQRKWKRKGTSCSSSSARINNLDDGCLMHIFSFLSPIPDRYNTALVCHRWRYLA 60
           VSVQEIKQRKWKRKGTSCSSSSARINNLDDGCLMHIFSFLSPIPDRYNTALVCHRWRYLA
Sbjct: 8   VSVQEIKQRKWKRKGTSCSSSSARINNLDDGCLMHIFSFLSPIPDRYNTALVCHRWRYLA 67

Query: 61  CHPRLWLRVERSIKDLSESGVFPTIEAAVAAARPGDTILIATGGVHSASNIQITKPLCLI 120
           CHPRLWLRVERSIKDLSESGVFPTIEAAVAAARPGDTILIATGGVHSASNIQITKPLCLI
Sbjct: 68  CHPRLWLRVERSIKDLSESGVFPTIEAAVAAARPGDTILIATGGVHSASNIQITKPLCLI 127

Query: 121 GGGELPEETTLFCTRGSESALEFLCTSKLSNLTVKAELGCCLLHRKGRLIIDGCVLQCES 180
           GGGELPEETTLFCTRGSESALEFLCTSKLSNLTVKAELGCCLLHRKGRLIIDGCVLQCES
Sbjct: 128 GGGELPEETTLFCTRGSESALEFLCTSKLSNLTVKAELGCCLLHRKGRLIIDGCVLQCES 187

Query: 181 NPLDYLSCPIVCSANPDKLLPSSVKGGYTDGVSVSQTRIEGGAKAVLTSEDLALQHVRVI 240
           NPLDYLSCPIVCSANPDKLLPSSVKGGYTDGVSVSQTRIEGGAKAVLTSEDLALQHVRVI
Sbjct: 188 NPLDYLSCPIVCSANPDKLLPSSVKGGYTDGVSVSQTRIEGGAKAVLTSEDLALQHVRVI 247

Query: 241 YARTALFFWFDVEYKL 257
           YARTALFFWFDVEY L
Sbjct: 248 YARTALFFWFDVEYNL 263

BLAST of MS004265 vs. NCBI nr
Match: XP_004142419.1 (F-box protein SKIP5 isoform X1 [Cucumis sativus] >KGN52308.1 hypothetical protein Csa_008966 [Cucumis sativus])

HSP 1 Score: 483.4 bits (1243), Expect = 1.2e-132
Identity = 239/256 (93.36%), Postives = 246/256 (96.09%), Query Frame = 0

Query: 1   VSVQEIKQRKWKRKGTSCSSSSARINNLDDGCLMHIFSFLSPIPDRYNTALVCHRWRYLA 60
           VS++E KQRKWKRKGT  SSSS  +N+LDDGCLMHIFSFLSPIPDRYNTALVCHRWRYLA
Sbjct: 19  VSMEENKQRKWKRKGT--SSSSPSVNSLDDGCLMHIFSFLSPIPDRYNTALVCHRWRYLA 78

Query: 61  CHPRLWLRVERSIKDLSESGVFPTIEAAVAAARPGDTILIATGGVHSASNIQITKPLCLI 120
           CHPRLWLRVERSIKDLSE GVFPTIEAAVAAARPGDTILIATGGVHSASNIQITKPLCLI
Sbjct: 79  CHPRLWLRVERSIKDLSEPGVFPTIEAAVAAARPGDTILIATGGVHSASNIQITKPLCLI 138

Query: 121 GGGELPEETTLFCTRGSESALEFLCTSKLSNLTVKAELGCCLLHRKGRLIIDGCVLQCES 180
           GGGELPEETTLFCTRGSESALEFL TSKLSNLTVKAELGCCLLHRKGRLI+DGCVLQCES
Sbjct: 139 GGGELPEETTLFCTRGSESALEFLSTSKLSNLTVKAELGCCLLHRKGRLIVDGCVLQCES 198

Query: 181 NPLDYLSCPIVCSANPDKLLPSSVKGGYTDGVSVSQTRIEGGAKAVLTSEDLALQHVRVI 240
           NPLDYLSCPIVC+A+PDKLLPSSVKGGYT GVSVS TRIEGGAKAVLTSEDL LQHVRVI
Sbjct: 199 NPLDYLSCPIVCTASPDKLLPSSVKGGYTHGVSVSHTRIEGGAKAVLTSEDLTLQHVRVI 258

Query: 241 YARTALFFWFDVEYKL 257
           YARTALFFWFDVEYKL
Sbjct: 259 YARTALFFWFDVEYKL 272

BLAST of MS004265 vs. NCBI nr
Match: XP_022966937.1 (F-box protein SKIP5 isoform X1 [Cucurbita maxima])

HSP 1 Score: 481.9 bits (1239), Expect = 3.5e-132
Identity = 236/255 (92.55%), Postives = 247/255 (96.86%), Query Frame = 0

Query: 2   SVQEIKQRKWKRKGTSCSSSSARINNLDDGCLMHIFSFLSPIPDRYNTALVCHRWRYLAC 61
           ++++ KQRKWKRKGT   SSSARINNLDDGCLMHIFSFLSPIPDRYNTALVCHRWRYLAC
Sbjct: 6   TMEDNKQRKWKRKGT--PSSSARINNLDDGCLMHIFSFLSPIPDRYNTALVCHRWRYLAC 65

Query: 62  HPRLWLRVERSIKDLSESGVFPTIEAAVAAARPGDTILIATGGVHSASNIQITKPLCLIG 121
           HPRLWLRVERSIKDLSESGVFPT+EAAVAAARPGDTILIATGGVHS SNIQITKPLCLIG
Sbjct: 66  HPRLWLRVERSIKDLSESGVFPTVEAAVAAARPGDTILIATGGVHSVSNIQITKPLCLIG 125

Query: 122 GGELPEETTLFCTRGSESALEFLCTSKLSNLTVKAELGCCLLHRKGRLIIDGCVLQCESN 181
           GGELPEETTLFC+RGSESALEFLCTSKLSNLTVKAELGCCLLHRKGRLIIDGCVLQCESN
Sbjct: 126 GGELPEETTLFCSRGSESALEFLCTSKLSNLTVKAELGCCLLHRKGRLIIDGCVLQCESN 185

Query: 182 PLDYLSCPIVCSANPDKLLPSSVKGGYTDGVSVSQTRIEGGAKAVLTSEDLALQHVRVIY 241
           PLDYLSCPIVC+A+PDKL  SSVKGGY DGVSVSQTRIEGGAKA+LTSEDLAL+HVRVIY
Sbjct: 186 PLDYLSCPIVCTASPDKLSSSSVKGGYADGVSVSQTRIEGGAKAILTSEDLALRHVRVIY 245

Query: 242 ARTALFFWFDVEYKL 257
           ARTALFFWFDVE+KL
Sbjct: 246 ARTALFFWFDVEHKL 258

BLAST of MS004265 vs. NCBI nr
Match: XP_008446937.1 (PREDICTED: F-box protein SKIP5 [Cucumis melo] >KAA0034751.1 F-box protein SKIP5 [Cucumis melo var. makuwa] >TYK09305.1 F-box protein SKIP5 [Cucumis melo var. makuwa])

HSP 1 Score: 479.9 bits (1234), Expect = 1.3e-131
Identity = 237/256 (92.58%), Postives = 245/256 (95.70%), Query Frame = 0

Query: 1   VSVQEIKQRKWKRKGTSCSSSSARINNLDDGCLMHIFSFLSPIPDRYNTALVCHRWRYLA 60
           VS++E KQRKWKRKGT  SSSS  +N+LDDGCLMHIFSFLSPIPDRYNTALVCHRWRYLA
Sbjct: 19  VSMEENKQRKWKRKGT--SSSSPSVNSLDDGCLMHIFSFLSPIPDRYNTALVCHRWRYLA 78

Query: 61  CHPRLWLRVERSIKDLSESGVFPTIEAAVAAARPGDTILIATGGVHSASNIQITKPLCLI 120
           CHPRLWLRVER+IKDLSE GVFPTIEAAVAAARPGDTILIATGGVHSASNIQITKPLCLI
Sbjct: 79  CHPRLWLRVERTIKDLSEPGVFPTIEAAVAAARPGDTILIATGGVHSASNIQITKPLCLI 138

Query: 121 GGGELPEETTLFCTRGSESALEFLCTSKLSNLTVKAELGCCLLHRKGRLIIDGCVLQCES 180
           GGGELPEETTLFCTRGSESALEFL TSKLSNLTVKAELGCCLLHRKGRLI+DGCVLQCES
Sbjct: 139 GGGELPEETTLFCTRGSESALEFLSTSKLSNLTVKAELGCCLLHRKGRLIVDGCVLQCES 198

Query: 181 NPLDYLSCPIVCSANPDKLLPSSVKGGYTDGVSVSQTRIEGGAKAVLTSEDLALQHVRVI 240
           NPLDYLSCPIVC+A+PDKLLPSSVKGGYT GVSVS TRIEGGAKAVLTSEDL LQHVRVI
Sbjct: 199 NPLDYLSCPIVCTASPDKLLPSSVKGGYTHGVSVSHTRIEGGAKAVLTSEDLTLQHVRVI 258

Query: 241 YARTALFFWFDVEYKL 257
           YARTAL FWFDVEYKL
Sbjct: 259 YARTALLFWFDVEYKL 272

BLAST of MS004265 vs. NCBI nr
Match: XP_038889674.1 (F-box protein SKIP5 [Benincasa hispida])

HSP 1 Score: 477.6 bits (1228), Expect = 6.6e-131
Identity = 238/256 (92.97%), Postives = 243/256 (94.92%), Query Frame = 0

Query: 1   VSVQEIKQRKWKRKGTSCSSSSARINNLDDGCLMHIFSFLSPIPDRYNTALVCHRWRYLA 60
           VS++E KQRKWKRKGTS SS S  INNLDDGCLMHIFSFLSP+PDRYNTALVCHRWRYLA
Sbjct: 14  VSMEESKQRKWKRKGTSTSSPS--INNLDDGCLMHIFSFLSPMPDRYNTALVCHRWRYLA 73

Query: 61  CHPRLWLRVERSIKDLSESGVFPTIEAAVAAARPGDTILIATGGVHSASNIQITKPLCLI 120
           CHPRLWLRVERSIKDLSE GVFPTIEAAVAAARPGDTILIATGGVHSASNIQITKPLCLI
Sbjct: 74  CHPRLWLRVERSIKDLSEPGVFPTIEAAVAAARPGDTILIATGGVHSASNIQITKPLCLI 133

Query: 121 GGGELPEETTLFCTRGSESALEFLCTSKLSNLTVKAELGCCLLHRKGRLIIDGCVLQCES 180
           GGGELPEETTLFCTRGSESALEFL TSKLSNLTVKAELGCCLLHRKGRLIIDGCVLQCES
Sbjct: 134 GGGELPEETTLFCTRGSESALEFLSTSKLSNLTVKAELGCCLLHRKGRLIIDGCVLQCES 193

Query: 181 NPLDYLSCPIVCSANPDKLLPSSVKGGYTDGVSVSQTRIEGGAKAVLTSEDLALQHVRVI 240
           NPLDYLSCPIVC+A PDKLLPSSV  GYT GVSVS TRIEGGAKAVLT +DLALQHVRVI
Sbjct: 194 NPLDYLSCPIVCTACPDKLLPSSVSRGYTHGVSVSHTRIEGGAKAVLTGDDLALQHVRVI 253

Query: 241 YARTALFFWFDVEYKL 257
           YARTALFFWFDVEYKL
Sbjct: 254 YARTALFFWFDVEYKL 267

BLAST of MS004265 vs. ExPASy Swiss-Prot
Match: Q94FT2 (F-box protein SKIP5 OS=Arabidopsis thaliana OX=3702 GN=SKIP5 PE=1 SV=1)

HSP 1 Score: 342.4 bits (877), Expect = 4.3e-93
Identity = 172/261 (65.90%), Postives = 204/261 (78.16%), Query Frame = 0

Query: 4   QEIKQRKWKRKGTSCSSSSARINNLDDGCLMHIFSFLSPIPDRYNTALVCHRWRYLACHP 63
           + I + K K++    S +S  +NNLDDGCLMHI SFLSPIPDRYNTALVCHRWRYLACHP
Sbjct: 16  ERIVESKMKKRMKPYSLTS--LNNLDDGCLMHILSFLSPIPDRYNTALVCHRWRYLACHP 75

Query: 64  RLWLRVERSIKDLSESGVFPTIEAAVAAARPGDTILIATGGVHSASNIQITKPLCLIGGG 123
           RLWLRV+R +KDLS+ GVF  IE+AV+AARPGDTILI  GG +  SNIQI KPLCL+GGG
Sbjct: 76  RLWLRVDRFVKDLSQPGVFLNIESAVSAARPGDTILIVAGGNYRVSNIQIKKPLCLVGGG 135

Query: 124 ELPEETTLFCTRGSESALEFLCTSKLSNLTVKAELGCCLLHRKGRLIIDGCVLQCESNPL 183
           E+P+ETTL C RGS+SALE L T KL+NLTVKAELGCCLLHR GRL IDGCVLQCE+NPL
Sbjct: 136 EIPDETTLVCARGSDSALELLSTCKLANLTVKAELGCCLLHRSGRLTIDGCVLQCETNPL 195

Query: 184 DYLSCPIVCSANPDKL--------LPSSVKGGY-TDGVSVSQTRIEGGAKAVLTSEDLAL 243
           D+LSCPIV +A  + +        +  +V G    + V+V QTRIEGGAKAV T  DL L
Sbjct: 196 DHLSCPIVSTAGDEDIENILSHVEVKETVTGKIKANSVTVLQTRIEGGAKAVSTRGDLVL 255

Query: 244 QHVRVIYARTALFFWFDVEYK 256
           Q VRV+Y++  L+FWFDV+Y+
Sbjct: 256 QRVRVMYSKAYLYFWFDVDYE 274

BLAST of MS004265 vs. ExPASy TrEMBL
Match: A0A6J1CBW5 (F-box protein SKIP5 OS=Momordica charantia OX=3673 GN=LOC111010147 PE=4 SV=1)

HSP 1 Score: 518.5 bits (1334), Expect = 1.6e-143
Identity = 255/256 (99.61%), Postives = 255/256 (99.61%), Query Frame = 0

Query: 1   VSVQEIKQRKWKRKGTSCSSSSARINNLDDGCLMHIFSFLSPIPDRYNTALVCHRWRYLA 60
           VSVQEIKQRKWKRKGTSCSSSSARINNLDDGCLMHIFSFLSPIPDRYNTALVCHRWRYLA
Sbjct: 8   VSVQEIKQRKWKRKGTSCSSSSARINNLDDGCLMHIFSFLSPIPDRYNTALVCHRWRYLA 67

Query: 61  CHPRLWLRVERSIKDLSESGVFPTIEAAVAAARPGDTILIATGGVHSASNIQITKPLCLI 120
           CHPRLWLRVERSIKDLSESGVFPTIEAAVAAARPGDTILIATGGVHSASNIQITKPLCLI
Sbjct: 68  CHPRLWLRVERSIKDLSESGVFPTIEAAVAAARPGDTILIATGGVHSASNIQITKPLCLI 127

Query: 121 GGGELPEETTLFCTRGSESALEFLCTSKLSNLTVKAELGCCLLHRKGRLIIDGCVLQCES 180
           GGGELPEETTLFCTRGSESALEFLCTSKLSNLTVKAELGCCLLHRKGRLIIDGCVLQCES
Sbjct: 128 GGGELPEETTLFCTRGSESALEFLCTSKLSNLTVKAELGCCLLHRKGRLIIDGCVLQCES 187

Query: 181 NPLDYLSCPIVCSANPDKLLPSSVKGGYTDGVSVSQTRIEGGAKAVLTSEDLALQHVRVI 240
           NPLDYLSCPIVCSANPDKLLPSSVKGGYTDGVSVSQTRIEGGAKAVLTSEDLALQHVRVI
Sbjct: 188 NPLDYLSCPIVCSANPDKLLPSSVKGGYTDGVSVSQTRIEGGAKAVLTSEDLALQHVRVI 247

Query: 241 YARTALFFWFDVEYKL 257
           YARTALFFWFDVEY L
Sbjct: 248 YARTALFFWFDVEYNL 263

BLAST of MS004265 vs. ExPASy TrEMBL
Match: A0A0A0KRR7 (F-box domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G623720 PE=4 SV=1)

HSP 1 Score: 483.4 bits (1243), Expect = 5.8e-133
Identity = 239/256 (93.36%), Postives = 246/256 (96.09%), Query Frame = 0

Query: 1   VSVQEIKQRKWKRKGTSCSSSSARINNLDDGCLMHIFSFLSPIPDRYNTALVCHRWRYLA 60
           VS++E KQRKWKRKGT  SSSS  +N+LDDGCLMHIFSFLSPIPDRYNTALVCHRWRYLA
Sbjct: 19  VSMEENKQRKWKRKGT--SSSSPSVNSLDDGCLMHIFSFLSPIPDRYNTALVCHRWRYLA 78

Query: 61  CHPRLWLRVERSIKDLSESGVFPTIEAAVAAARPGDTILIATGGVHSASNIQITKPLCLI 120
           CHPRLWLRVERSIKDLSE GVFPTIEAAVAAARPGDTILIATGGVHSASNIQITKPLCLI
Sbjct: 79  CHPRLWLRVERSIKDLSEPGVFPTIEAAVAAARPGDTILIATGGVHSASNIQITKPLCLI 138

Query: 121 GGGELPEETTLFCTRGSESALEFLCTSKLSNLTVKAELGCCLLHRKGRLIIDGCVLQCES 180
           GGGELPEETTLFCTRGSESALEFL TSKLSNLTVKAELGCCLLHRKGRLI+DGCVLQCES
Sbjct: 139 GGGELPEETTLFCTRGSESALEFLSTSKLSNLTVKAELGCCLLHRKGRLIVDGCVLQCES 198

Query: 181 NPLDYLSCPIVCSANPDKLLPSSVKGGYTDGVSVSQTRIEGGAKAVLTSEDLALQHVRVI 240
           NPLDYLSCPIVC+A+PDKLLPSSVKGGYT GVSVS TRIEGGAKAVLTSEDL LQHVRVI
Sbjct: 199 NPLDYLSCPIVCTASPDKLLPSSVKGGYTHGVSVSHTRIEGGAKAVLTSEDLTLQHVRVI 258

Query: 241 YARTALFFWFDVEYKL 257
           YARTALFFWFDVEYKL
Sbjct: 259 YARTALFFWFDVEYKL 272

BLAST of MS004265 vs. ExPASy TrEMBL
Match: A0A6J1HPC3 (F-box protein SKIP5 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111466500 PE=4 SV=1)

HSP 1 Score: 481.9 bits (1239), Expect = 1.7e-132
Identity = 236/255 (92.55%), Postives = 247/255 (96.86%), Query Frame = 0

Query: 2   SVQEIKQRKWKRKGTSCSSSSARINNLDDGCLMHIFSFLSPIPDRYNTALVCHRWRYLAC 61
           ++++ KQRKWKRKGT   SSSARINNLDDGCLMHIFSFLSPIPDRYNTALVCHRWRYLAC
Sbjct: 6   TMEDNKQRKWKRKGT--PSSSARINNLDDGCLMHIFSFLSPIPDRYNTALVCHRWRYLAC 65

Query: 62  HPRLWLRVERSIKDLSESGVFPTIEAAVAAARPGDTILIATGGVHSASNIQITKPLCLIG 121
           HPRLWLRVERSIKDLSESGVFPT+EAAVAAARPGDTILIATGGVHS SNIQITKPLCLIG
Sbjct: 66  HPRLWLRVERSIKDLSESGVFPTVEAAVAAARPGDTILIATGGVHSVSNIQITKPLCLIG 125

Query: 122 GGELPEETTLFCTRGSESALEFLCTSKLSNLTVKAELGCCLLHRKGRLIIDGCVLQCESN 181
           GGELPEETTLFC+RGSESALEFLCTSKLSNLTVKAELGCCLLHRKGRLIIDGCVLQCESN
Sbjct: 126 GGELPEETTLFCSRGSESALEFLCTSKLSNLTVKAELGCCLLHRKGRLIIDGCVLQCESN 185

Query: 182 PLDYLSCPIVCSANPDKLLPSSVKGGYTDGVSVSQTRIEGGAKAVLTSEDLALQHVRVIY 241
           PLDYLSCPIVC+A+PDKL  SSVKGGY DGVSVSQTRIEGGAKA+LTSEDLAL+HVRVIY
Sbjct: 186 PLDYLSCPIVCTASPDKLSSSSVKGGYADGVSVSQTRIEGGAKAILTSEDLALRHVRVIY 245

Query: 242 ARTALFFWFDVEYKL 257
           ARTALFFWFDVE+KL
Sbjct: 246 ARTALFFWFDVEHKL 258

BLAST of MS004265 vs. ExPASy TrEMBL
Match: A0A5A7SZI1 (F-box protein SKIP5 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold475G003020 PE=4 SV=1)

HSP 1 Score: 479.9 bits (1234), Expect = 6.4e-132
Identity = 237/256 (92.58%), Postives = 245/256 (95.70%), Query Frame = 0

Query: 1   VSVQEIKQRKWKRKGTSCSSSSARINNLDDGCLMHIFSFLSPIPDRYNTALVCHRWRYLA 60
           VS++E KQRKWKRKGT  SSSS  +N+LDDGCLMHIFSFLSPIPDRYNTALVCHRWRYLA
Sbjct: 19  VSMEENKQRKWKRKGT--SSSSPSVNSLDDGCLMHIFSFLSPIPDRYNTALVCHRWRYLA 78

Query: 61  CHPRLWLRVERSIKDLSESGVFPTIEAAVAAARPGDTILIATGGVHSASNIQITKPLCLI 120
           CHPRLWLRVER+IKDLSE GVFPTIEAAVAAARPGDTILIATGGVHSASNIQITKPLCLI
Sbjct: 79  CHPRLWLRVERTIKDLSEPGVFPTIEAAVAAARPGDTILIATGGVHSASNIQITKPLCLI 138

Query: 121 GGGELPEETTLFCTRGSESALEFLCTSKLSNLTVKAELGCCLLHRKGRLIIDGCVLQCES 180
           GGGELPEETTLFCTRGSESALEFL TSKLSNLTVKAELGCCLLHRKGRLI+DGCVLQCES
Sbjct: 139 GGGELPEETTLFCTRGSESALEFLSTSKLSNLTVKAELGCCLLHRKGRLIVDGCVLQCES 198

Query: 181 NPLDYLSCPIVCSANPDKLLPSSVKGGYTDGVSVSQTRIEGGAKAVLTSEDLALQHVRVI 240
           NPLDYLSCPIVC+A+PDKLLPSSVKGGYT GVSVS TRIEGGAKAVLTSEDL LQHVRVI
Sbjct: 199 NPLDYLSCPIVCTASPDKLLPSSVKGGYTHGVSVSHTRIEGGAKAVLTSEDLTLQHVRVI 258

Query: 241 YARTALFFWFDVEYKL 257
           YARTAL FWFDVEYKL
Sbjct: 259 YARTALLFWFDVEYKL 272

BLAST of MS004265 vs. ExPASy TrEMBL
Match: A0A1S3BG99 (F-box protein SKIP5 OS=Cucumis melo OX=3656 GN=LOC103489501 PE=4 SV=1)

HSP 1 Score: 479.9 bits (1234), Expect = 6.4e-132
Identity = 237/256 (92.58%), Postives = 245/256 (95.70%), Query Frame = 0

Query: 1   VSVQEIKQRKWKRKGTSCSSSSARINNLDDGCLMHIFSFLSPIPDRYNTALVCHRWRYLA 60
           VS++E KQRKWKRKGT  SSSS  +N+LDDGCLMHIFSFLSPIPDRYNTALVCHRWRYLA
Sbjct: 19  VSMEENKQRKWKRKGT--SSSSPSVNSLDDGCLMHIFSFLSPIPDRYNTALVCHRWRYLA 78

Query: 61  CHPRLWLRVERSIKDLSESGVFPTIEAAVAAARPGDTILIATGGVHSASNIQITKPLCLI 120
           CHPRLWLRVER+IKDLSE GVFPTIEAAVAAARPGDTILIATGGVHSASNIQITKPLCLI
Sbjct: 79  CHPRLWLRVERTIKDLSEPGVFPTIEAAVAAARPGDTILIATGGVHSASNIQITKPLCLI 138

Query: 121 GGGELPEETTLFCTRGSESALEFLCTSKLSNLTVKAELGCCLLHRKGRLIIDGCVLQCES 180
           GGGELPEETTLFCTRGSESALEFL TSKLSNLTVKAELGCCLLHRKGRLI+DGCVLQCES
Sbjct: 139 GGGELPEETTLFCTRGSESALEFLSTSKLSNLTVKAELGCCLLHRKGRLIVDGCVLQCES 198

Query: 181 NPLDYLSCPIVCSANPDKLLPSSVKGGYTDGVSVSQTRIEGGAKAVLTSEDLALQHVRVI 240
           NPLDYLSCPIVC+A+PDKLLPSSVKGGYT GVSVS TRIEGGAKAVLTSEDL LQHVRVI
Sbjct: 199 NPLDYLSCPIVCTASPDKLLPSSVKGGYTHGVSVSHTRIEGGAKAVLTSEDLTLQHVRVI 258

Query: 241 YARTALFFWFDVEYKL 257
           YARTAL FWFDVEYKL
Sbjct: 259 YARTALLFWFDVEYKL 272

BLAST of MS004265 vs. TAIR 10
Match: AT3G54480.1 (SKP1/ASK-interacting protein 5 )

HSP 1 Score: 342.4 bits (877), Expect = 3.1e-94
Identity = 172/261 (65.90%), Postives = 204/261 (78.16%), Query Frame = 0

Query: 4   QEIKQRKWKRKGTSCSSSSARINNLDDGCLMHIFSFLSPIPDRYNTALVCHRWRYLACHP 63
           + I + K K++    S +S  +NNLDDGCLMHI SFLSPIPDRYNTALVCHRWRYLACHP
Sbjct: 16  ERIVESKMKKRMKPYSLTS--LNNLDDGCLMHILSFLSPIPDRYNTALVCHRWRYLACHP 75

Query: 64  RLWLRVERSIKDLSESGVFPTIEAAVAAARPGDTILIATGGVHSASNIQITKPLCLIGGG 123
           RLWLRV+R +KDLS+ GVF  IE+AV+AARPGDTILI  GG +  SNIQI KPLCL+GGG
Sbjct: 76  RLWLRVDRFVKDLSQPGVFLNIESAVSAARPGDTILIVAGGNYRVSNIQIKKPLCLVGGG 135

Query: 124 ELPEETTLFCTRGSESALEFLCTSKLSNLTVKAELGCCLLHRKGRLIIDGCVLQCESNPL 183
           E+P+ETTL C RGS+SALE L T KL+NLTVKAELGCCLLHR GRL IDGCVLQCE+NPL
Sbjct: 136 EIPDETTLVCARGSDSALELLSTCKLANLTVKAELGCCLLHRSGRLTIDGCVLQCETNPL 195

Query: 184 DYLSCPIVCSANPDKL--------LPSSVKGGY-TDGVSVSQTRIEGGAKAVLTSEDLAL 243
           D+LSCPIV +A  + +        +  +V G    + V+V QTRIEGGAKAV T  DL L
Sbjct: 196 DHLSCPIVSTAGDEDIENILSHVEVKETVTGKIKANSVTVLQTRIEGGAKAVSTRGDLVL 255

Query: 244 QHVRVIYARTALFFWFDVEYK 256
           Q VRV+Y++  L+FWFDV+Y+
Sbjct: 256 QRVRVMYSKAYLYFWFDVDYE 274

BLAST of MS004265 vs. TAIR 10
Match: AT3G54480.2 (SKP1/ASK-interacting protein 5 )

HSP 1 Score: 132.1 bits (331), Expect = 6.3e-31
Identity = 63/90 (70.00%), Postives = 74/90 (82.22%), Query Frame = 0

Query: 4   QEIKQRKWKRKGTSCSSSSARINNLDDGCLMHIFSFLSPIPDRYNTALVCHRWRYLACHP 63
           + I + K K++    S +S  +NNLDDGCLMHI SFLSPIPDRYNTALVCHRWRYLACHP
Sbjct: 16  ERIVESKMKKRMKPYSLTS--LNNLDDGCLMHILSFLSPIPDRYNTALVCHRWRYLACHP 75

Query: 64  RLWLRVERSIKDLSESGVFPTIEAAVAAAR 94
           RLWLRV+R +KDLS+ GVF  IE+AV+AAR
Sbjct: 76  RLWLRVDRFVKDLSQPGVFLNIESAVSAAR 103

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022139176.13.4e-14399.61F-box protein SKIP5 [Momordica charantia][more]
XP_004142419.11.2e-13293.36F-box protein SKIP5 isoform X1 [Cucumis sativus] >KGN52308.1 hypothetical protei... [more]
XP_022966937.13.5e-13292.55F-box protein SKIP5 isoform X1 [Cucurbita maxima][more]
XP_008446937.11.3e-13192.58PREDICTED: F-box protein SKIP5 [Cucumis melo] >KAA0034751.1 F-box protein SKIP5 ... [more]
XP_038889674.16.6e-13192.97F-box protein SKIP5 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
Q94FT24.3e-9365.90F-box protein SKIP5 OS=Arabidopsis thaliana OX=3702 GN=SKIP5 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1CBW51.6e-14399.61F-box protein SKIP5 OS=Momordica charantia OX=3673 GN=LOC111010147 PE=4 SV=1[more]
A0A0A0KRR75.8e-13393.36F-box domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G623720 PE=4 ... [more]
A0A6J1HPC31.7e-13292.55F-box protein SKIP5 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111466500 PE=4 ... [more]
A0A5A7SZI16.4e-13292.58F-box protein SKIP5 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold475G... [more]
A0A1S3BG996.4e-13292.58F-box protein SKIP5 OS=Cucumis melo OX=3656 GN=LOC103489501 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT3G54480.13.1e-9465.90SKP1/ASK-interacting protein 5 [more]
AT3G54480.26.3e-3170.00SKP1/ASK-interacting protein 5 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001810F-box domainSMARTSM00256fbox_2coord: 28..69
e-value: 0.0065
score: 25.7
IPR001810F-box domainPFAMPF12937F-box-likecoord: 32..69
e-value: 6.6E-9
score: 35.5
NoneNo IPR availableGENE3D1.20.1280.50coord: 26..66
e-value: 2.4E-12
score: 48.5
NoneNo IPR availablePANTHERPTHR14695:SF4PROTEIN NESSUN DORMAcoord: 19..72
NoneNo IPR availablePANTHERPTHR14695:SF4PROTEIN NESSUN DORMAcoord: 73..255
IPR045140SHC SH2 domain-binding protein 1-likePANTHERPTHR14695SHC SH2-DOMAIN BINDING PROTEIN 1-RELATEDcoord: 19..72
IPR045140SHC SH2 domain-binding protein 1-likePANTHERPTHR14695SHC SH2-DOMAIN BINDING PROTEIN 1-RELATEDcoord: 73..255
IPR036047F-box-like domain superfamilySUPERFAMILY81383F-box domaincoord: 15..77
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 79..214

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS004265.1MS004265.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005515 protein binding