MS004227 (gene) Bitter gourd (TR) v1

Overview
NameMS004227
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionmRNA-decapping enzyme subunit 2-like
Locationscaffold92: 982721 .. 987038 (+)
RNA-Seq ExpressionMS004227
SyntenyMS004227
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCGGCCTCCATCAGCCCTCAACTGCTCCGGTGAAGAACGGTCTCCCTCCCCAAGAACTCCTCGACGATCTTTGCAGGTCTTCTCCGCACTCTCTCTCTATTCGATTTACTGGTTTTGCTACAAGTTCTGTGTTCGTTAGGTGTGGAGATCTTAGGGTTTTTAAGTGGAAATTGCTATGAGCTTGTTTATTTTCATTATCTTTCACATTTTTTTTTCATTTATTGCTTCCGCCGACTGAATGTCGTATTTAGCTTTTTGCAAAACGAAGCACGATGGTAAACATAGCGCCATGAACGGGCCAATAAGAATTATTCTAAAAGTCGCTTGGTTTTGTCGTTGAACGTACTTTGCCGACATATCTTCATTAAAATGAGTGAATTGTCAGTTTAGTTAGTTGGCAACTGTTCTTATTGATTATGAAAAATATATTGTAGTAAATGGGGATTCAGTTAGATATTTTTATTGGTTTAAGATGCTATATTAGTTTAGTTTGTCAGTTCTGATGGCCTTAATATGAGGGTGAGCATTTATTTTCTTTTGCCAAAACAAACGAGATACTAAATCACAAACCACTTAGAGAGACAATTAGGAAGATGGATATCTGACATAAACTTAATAATTTCCTTTTTACTAGTGTTTGTATTATTGAAATATGATATATAAGATATTGGTCGAAATGGATAGATAGAATGCAAAAAGAATTTCCTCCATGCAACCGAAACAGCATCAGCCATTTTGGAGGAAAGAATTGTTTTGAGTATTTTCTTAAGATTTATGAGCATTTTTCTTTTGGATTATTTTGCCTATTGCAATGTTATAGCTCCAACATTCTCATCTATCTATCACTTTTTCTCGATATGCAGCCGATTTGTGTTAAATGTGCCAAAGGAAGATCTACAATCATTCGAGAGGATTCTGTTTCTAGTTGAATATGCACATTGGTTCTATGAAGACAACTCAGTAGAAAATAATCCTTCACTGAAGTCATTTAATTTGAAGGAGTTCACTTCGTTATGTATCCTTGGCAATGGCATTATATGATCCGTTGTCCTTATAATGTGTTTGGATGTTTTAAAATTTCCATTTCTATGTTTAATAAATGAAAATTCCTTCTTCCTTTTGAATAAGTATATTATGTTTCTATTGTAAAATAAAACAACAAAGTCGTGAGTTTGCAATTTAGTATGAGGTTTACTCACTAGCAATTAAAAAAAGCCTAGTATCATAAGTTGTTAAGTTTACATATGATAAAGGGTAAATTACACACGAAAAGTGACTCTCATTCAATATTTTATGAAGATATATATACATTACGGTCATTCTTCATTATCAACTACCAAACTGAACAACTTGATATTACTAATGTCAGTCATTGTTGATACATCTTGAGATGCCAACTCATGGATGATACAGTCCGAGTCAGGTCGAATCACCCAATTTTTTGGTTTTCTTTGTGACATATCTCATACTCATCTTTTGAGACGGTTATGGCTTGGTTGAGTCTGAGTATCCATGACTTCCACAGCCCTGGGGACCTTTGTCACGTTATTATTTTCTTTTATATATACATGATATAGTTTTGTAAATTTTTTAAATTAATTAAGCTTCTTGTGGTGAAGTTTAAATATTTTTACTACATGCAAAGGCGGATGTCTTAATTTAAAAATTAAGATGATGCCAAGGTATAACCATAATCTAATTCTATACTGTTTTTTCTGGATATACTTGTATTCCTTAATTTTCTGCAGTATTTAACAGCTGTGATGTTTTGAAACCATATGTGCCCCACATAGATGACATATTTAAGGACTTCACTTCCTATAAGCTTCGAGTTCCTGTCACCGGGGCCATTATTTTAGATGAAACCTTTGAGAGGGTGAGTGTTGATTTGCAGATTTCTTTTATTAATACTGTTATTGATTTATTTGTATTAATTGATTGAATTATTTTGTTTTGTACACTTCCCATGTTCGTGCTTCCTTTTCTGCAACCAAACAGTGCTTACTAGTAAAGGGATGGAAAGGGTCAAGCTGGAGTTTTCCTCGTGGCAAAAAGAGCAAGGATGAAGAAGATCATGCTTGTGCCATTAGAGAAGTAATATTTCTCTTTCTTTTTGGGACTCTCATGACGCTACTTAAAAATTCTTTTCAGTGCCCCTTTTTTTTATTATTATTAATGAAAATGCCTGCTTTATTTGAGAAAAATGATATACTATAAAAAGAAAAAAAGAAAAGGAAAGTCCAAGACAACAAAAGCTTGAAAAAAAAAGGCTAGCTAATACAAAATTATTACAGCAAAGGGCTCTAGTTGATAAAGAGTGAGAGAAAAAGGAAAAATATTAAAAGATTTAGTTAAAGAAACTCAAAGGGAAGCATTGAACCCAGCCCTTCACCCATGATTCTTTGCACCTCTAAAAATTCTCCTATTTCTCTTGAGCCAAATGTTCCAAGAACGCAATGAAAACAGCCCACCAACAAGCTGCTTTGCTATCATGGAAAGAAGGATGAAATAACGCCTCGATACCTCCTAGGCCCTTAGTGGTAATGGAACCTCCAATGACATAAAGCTTCCGTTGATTAAAGTTTTGTGGACTTGAACCAGTTATTTGCCATCTGGTATGATTTGTGCTAAGCAAAAAGGAGTATTGAAGAGGTTTAATTGTTTCAGGGTTTTAGCTTAAATTACTGAATTGCTTATATTTGTACCAAAACAATTCTTTACTTCGCCACTTGTCCATGGGATTTATTTTTCATTGAGAATGCAGGTTTTGGAGGAAACTGGTTTTGACGTGTCAGCATTCCTTATCAAAGAGGATTTTATTGAATTGATGTTTGGACAACAAAGAGTGCGGCTCTACATTATCGCTGGGGTGAAAAACGATTCTTCCTTTGCGCCACTTACTAAGAAAGAGATCAGTGTATGTTTGCCTTCTCAATTTTTGTCTTCTTCAACTTAGAAATTCTTGAACAAAGATTTTGGATTTTCCTTTTCAATAATGGACAGATGGAAGACTTTAGGTTGATCCTTTTTATATTTAACATGCTATTGGGTTCAAAGCTATTTTGGTTCTGTTTTCTAGCTTTCGATCTTTATTTACAACGTTTATACTGAGCATTGACAATTTGGTAATTGGTATACCTTTCAACATCTGAGTTCAAGTTATCAGTACTTCCTATCTTCTAAGTGCCTCTATTGATTGTTTCATTCATGTTTGTTCCTCTTAAGGCCGAATTCTTAGAAAAATATATCATTCTATAATGCAAAGTAATGACCCAAATGACATATGGTTATGTTTTTTGGAAGCAGGAAATTGCATGGCACCGACTTGACGACCTTCTACCTGCCAGTGATGATGTGATATCTCATGGAATCACTGGTCTCAAGCTTTATATGGTGGCTCCTTTCTTAACGTGAGCAAGCATCGTTTTGTAGTTTTCTTGATTTTCCTAAGCTCGCAGTTTAGTTTATGTTTAGTGGAATCAACATTTTGATGCAGGTCACTGAGATCTTGGATTCTGACACATCAGCGTCCTGTGGTTCCTAATTTTGACATGCCTCTTAAAGGTAATCAGTTTGTGACTTGCAAATCAGGACCTTTAGTTTTGATTGTCAAATATAATTTCGATTGTTTGCTAGCTCTTCTTAAATAAAGAACTGGAAGGAACAAAAATGGTATCAAATATTTGGAGCTTTTTCTTTATTAAATCTAAATACAATTCAGGGAACATTTCTGAAAAGTAGTGTTGTCAAGCAAAAATAACCTTCAAAATTAAAATAGAAACCGAAAAACGGGTAAATGCAGATGTCATGACCAGTGATCAAGGATATTTTAGGTGAAAATAGGGACCTGATATCATTCTTTTCGATGGAATTTCATGTGCAAACAGCCAAGATATTAGTGGGAGAACTGAACAAATTGGTGTGACAATATGATCATTCAGCTACCATCCATGTGAAAATTAGATGCAACGTTTCTCTGTCATATCTCTGTCTGGTCTTGCAATACACTTGGCTTATTGACTTTCTCGTGTTCTTTGCCAAAAAAAAAATTTATAATAATAAAAATTGATATACACTTGGCTCATCAGACTTTCTCGTGTTCTTTGCCAATGAGCTCTGTTGACACCATTATAAACTTTTGCTATTCTCTCAGGAGTCACCATGTGGAAGGTGAAGAATAGCTCGATGAGTAGTAGCTCGATGGTATTAGACAACCAATCCAGTAAATCGGATTCTGATCCAGGAAAGAGCTTCAGAAACTTCAAATTCGATACTGCCTCAATCTTGCAGGCCATGGAAAGTGCTTTTGCTGCT

mRNA sequence

ATGGCCGGCCTCCATCAGCCCTCAACTGCTCCGGTGAAGAACGGTCTCCCTCCCCAAGAACTCCTCGACGATCTTTGCAGCCGATTTGTGTTAAATGTGCCAAAGGAAGATCTACAATCATTCGAGAGGATTCTGTTTCTAGTTGAATATGCACATTGGTTCTATGAAGACAACTCAGTAGAAAATAATCCTTCACTGAAGTCATTTAATTTGAAGGAGTTCACTTCGTTATTATTTAACAGCTGTGATGTTTTGAAACCATATGTGCCCCACATAGATGACATATTTAAGGACTTCACTTCCTATAAGCTTCGAGTTCCTGTCACCGGGGCCATTATTTTAGATGAAACCTTTGAGAGGTGCTTACTAGTAAAGGGATGGAAAGGGTCAAGCTGGAGTTTTCCTCGTGGCAAAAAGAGCAAGGATGAAGAAGATCATGCTTGTGCCATTAGAGAAGTTTTGGAGGAAACTGGTTTTGACGTGTCAGCATTCCTTATCAAAGAGGATTTTATTGAATTGATGTTTGGACAACAAAGAGTGCGGCTCTACATTATCGCTGGGGTGAAAAACGATTCTTCCTTTGCGCCACTTACTAAGAAAGAGATCAGTGAAATTGCATGGCACCGACTTGACGACCTTCTACCTGCCAGTGATGATGTGATATCTCATGGAATCACTGGTCTCAAGCTTTATATGGTGGCTCCTTTCTTAACGTCACTGAGATCTTGGATTCTGACACATCAGCGTCCTGTGGTTCCTAATTTTGACATGCCTCTTAAAGGAGTCACCATGTGGAAGGTGAAGAATAGCTCGATGAGTAGTAGCTCGATGGTATTAGACAACCAATCCAGTAAATCGGATTCTGATCCAGGAAAGAGCTTCAGAAACTTCAAATTCGATACTGCCTCAATCTTGCAGGCCATGGAAAGTGCTTTTGCTGCT

Coding sequence (CDS)

ATGGCCGGCCTCCATCAGCCCTCAACTGCTCCGGTGAAGAACGGTCTCCCTCCCCAAGAACTCCTCGACGATCTTTGCAGCCGATTTGTGTTAAATGTGCCAAAGGAAGATCTACAATCATTCGAGAGGATTCTGTTTCTAGTTGAATATGCACATTGGTTCTATGAAGACAACTCAGTAGAAAATAATCCTTCACTGAAGTCATTTAATTTGAAGGAGTTCACTTCGTTATTATTTAACAGCTGTGATGTTTTGAAACCATATGTGCCCCACATAGATGACATATTTAAGGACTTCACTTCCTATAAGCTTCGAGTTCCTGTCACCGGGGCCATTATTTTAGATGAAACCTTTGAGAGGTGCTTACTAGTAAAGGGATGGAAAGGGTCAAGCTGGAGTTTTCCTCGTGGCAAAAAGAGCAAGGATGAAGAAGATCATGCTTGTGCCATTAGAGAAGTTTTGGAGGAAACTGGTTTTGACGTGTCAGCATTCCTTATCAAAGAGGATTTTATTGAATTGATGTTTGGACAACAAAGAGTGCGGCTCTACATTATCGCTGGGGTGAAAAACGATTCTTCCTTTGCGCCACTTACTAAGAAAGAGATCAGTGAAATTGCATGGCACCGACTTGACGACCTTCTACCTGCCAGTGATGATGTGATATCTCATGGAATCACTGGTCTCAAGCTTTATATGGTGGCTCCTTTCTTAACGTCACTGAGATCTTGGATTCTGACACATCAGCGTCCTGTGGTTCCTAATTTTGACATGCCTCTTAAAGGAGTCACCATGTGGAAGGTGAAGAATAGCTCGATGAGTAGTAGCTCGATGGTATTAGACAACCAATCCAGTAAATCGGATTCTGATCCAGGAAAGAGCTTCAGAAACTTCAAATTCGATACTGCCTCAATCTTGCAGGCCATGGAAAGTGCTTTTGCTGCT

Protein sequence

MAGLHQPSTAPVKNGLPPQELLDDLCSRFVLNVPKEDLQSFERILFLVEYAHWFYEDNSVENNPSLKSFNLKEFTSLLFNSCDVLKPYVPHIDDIFKDFTSYKLRVPVTGAIILDETFERCLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDVSAFLIKEDFIELMFGQQRVRLYIIAGVKNDSSFAPLTKKEISEIAWHRLDDLLPASDDVISHGITGLKLYMVAPFLTSLRSWILTHQRPVVPNFDMPLKGVTMWKVKNSSMSSSSMVLDNQSSKSDSDPGKSFRNFKFDTASILQAMESAFAA
Homology
BLAST of MS004227 vs. NCBI nr
Match: XP_022139266.1 (mRNA-decapping enzyme subunit 2-like [Momordica charantia])

HSP 1 Score: 630.6 bits (1625), Expect = 7.4e-177
Identity = 312/314 (99.36%), Postives = 312/314 (99.36%), Query Frame = 0

Query: 1   MAGLHQPSTAPVKNGLPPQELLDDLCSRFVLNVPKEDLQSFERILFLVEYAHWFYEDNSV 60
           MAGLHQPS APVKNGLPPQELLDDLCSRFVLNVPKEDLQSFERILFLVEYAHWFYEDNSV
Sbjct: 1   MAGLHQPSIAPVKNGLPPQELLDDLCSRFVLNVPKEDLQSFERILFLVEYAHWFYEDNSV 60

Query: 61  ENNPSLKSFNLKEFTSLLFNSCDVLKPYVPHIDDIFKDFTSYKLRVPVTGAIILDETFER 120
           ENNPSLKSFNLKEFTSLLFNSCDVLKPYVPHIDDIFKDFTSYKLRVPVTGAIILDETFER
Sbjct: 61  ENNPSLKSFNLKEFTSLLFNSCDVLKPYVPHIDDIFKDFTSYKLRVPVTGAIILDETFER 120

Query: 121 CLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDVSAFLIKEDFIELMFGQQRV 180
           CLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDVSAFLIKEDFIELMFGQQRV
Sbjct: 121 CLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDVSAFLIKEDFIELMFGQQRV 180

Query: 181 RLYIIAGVKNDSSFAPLTKKEISEIAWHRLDDLLPASDDVISHGITGLKLYMVAPFLTSL 240
           RLYIIAGVKNDSSFAPLTKKEISEIAWHRLDDLLPASDDVISHGITGLKLYMVAPFLTSL
Sbjct: 181 RLYIIAGVKNDSSFAPLTKKEISEIAWHRLDDLLPASDDVISHGITGLKLYMVAPFLTSL 240

Query: 241 RSWILTHQRPVVPNFDMPLKGVTMWKVKNSSMSSSSMVLDNQSSKSDSDPGKSFRNFKFD 300
           RSWILTHQRPVVPNFDMPLKGVTMWKVKNSSMSSSSMVLDNQSSKSDSDPGKSFRNFKFD
Sbjct: 241 RSWILTHQRPVVPNFDMPLKGVTMWKVKNSSMSSSSMVLDNQSSKSDSDPGKSFRNFKFD 300

Query: 301 TASILQAMESAFAA 315
           TASILQAME AFAA
Sbjct: 301 TASILQAMEGAFAA 314

BLAST of MS004227 vs. NCBI nr
Match: XP_038891421.1 (mRNA-decapping enzyme subunit 2-like isoform X2 [Benincasa hispida])

HSP 1 Score: 591.3 bits (1523), Expect = 5.0e-165
Identity = 289/314 (92.04%), Postives = 299/314 (95.22%), Query Frame = 0

Query: 1   MAGLHQPSTAPVKNGLPPQELLDDLCSRFVLNVPKEDLQSFERILFLVEYAHWFYEDNSV 60
           MAGLH PS APVKNGLPPQELLDDLCSRFVLNVPKEDLQSFERILFLVEYAHWFYEDNSV
Sbjct: 1   MAGLHLPSNAPVKNGLPPQELLDDLCSRFVLNVPKEDLQSFERILFLVEYAHWFYEDNSV 60

Query: 61  ENNPSLKSFNLKEFTSLLFNSCDVLKPYVPHIDDIFKDFTSYKLRVPVTGAIILDETFER 120
           ENNPSLKSFNLKEFTSLLFNSCDVLKPYVPHIDDIFKDFTSYKLRVPVTG IILDETFER
Sbjct: 61  ENNPSLKSFNLKEFTSLLFNSCDVLKPYVPHIDDIFKDFTSYKLRVPVTGGIILDETFER 120

Query: 121 CLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDVSAFLIKEDFIELMFGQQRV 180
           CLLVKGWKGSSWSFPRGKKSKDEEDHACAIREV EETGFDV+AFLIKEDFIE+MFGQQRV
Sbjct: 121 CLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVSEETGFDVTAFLIKEDFIEVMFGQQRV 180

Query: 181 RLYIIAGVKNDSSFAPLTKKEISEIAWHRLDDLLPASDDVISHGITGLKLYMVAPFLTSL 240
           RLYIIAGVKND++FAPLTKKEISEIAWHRLDDLLP SDDVISHGITGLKLYMVAPFL SL
Sbjct: 181 RLYIIAGVKNDTAFAPLTKKEISEIAWHRLDDLLPVSDDVISHGITGLKLYMVAPFLESL 240

Query: 241 RSWILTHQRPVVPNFDMPLKGVTMWKVKNSSMSSSSMVLDNQSSKSDSDPGKSFRNFKFD 300
           RSWIL HQ PV PNFDMP+KGVT+WKVKNSSMS+SS++ D QS K DSDPG+S RNFKFD
Sbjct: 241 RSWILKHQPPVAPNFDMPVKGVTIWKVKNSSMSNSSIIFDGQSIKLDSDPGRSLRNFKFD 300

Query: 301 TASILQAMESAFAA 315
           TASIL AMESAFAA
Sbjct: 301 TASILHAMESAFAA 314

BLAST of MS004227 vs. NCBI nr
Match: XP_004142410.1 (mRNA-decapping enzyme subunit 2 [Cucumis sativus] >XP_031742317.1 mRNA-decapping enzyme subunit 2 [Cucumis sativus] >KGN52271.1 hypothetical protein Csa_007962 [Cucumis sativus])

HSP 1 Score: 587.0 bits (1512), Expect = 9.4e-164
Identity = 287/314 (91.40%), Postives = 297/314 (94.59%), Query Frame = 0

Query: 1   MAGLHQPSTAPVKNGLPPQELLDDLCSRFVLNVPKEDLQSFERILFLVEYAHWFYEDNSV 60
           MAGLH PS A VKNGLPPQELLDDLCSRFVLNVPKEDLQSFERILFLVEYAHWFYEDNSV
Sbjct: 1   MAGLHLPSNAHVKNGLPPQELLDDLCSRFVLNVPKEDLQSFERILFLVEYAHWFYEDNSV 60

Query: 61  ENNPSLKSFNLKEFTSLLFNSCDVLKPYVPHIDDIFKDFTSYKLRVPVTGAIILDETFER 120
           ENNPSLKSFNLKEFTSLLFNSCDVLKPYVPHIDDIFKDFTSYKLRVPVTG IILDETFER
Sbjct: 61  ENNPSLKSFNLKEFTSLLFNSCDVLKPYVPHIDDIFKDFTSYKLRVPVTGGIILDETFER 120

Query: 121 CLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDVSAFLIKEDFIELMFGQQRV 180
           CLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDV+ FLIKEDFIE+MFGQQRV
Sbjct: 121 CLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDVTPFLIKEDFIEVMFGQQRV 180

Query: 181 RLYIIAGVKNDSSFAPLTKKEISEIAWHRLDDLLPASDDVISHGITGLKLYMVAPFLTSL 240
           RLYIIAGVKND++FAPLTKKEISEIAWHRLDDLLP SDDVISHGITGLKLYMVAPFL SL
Sbjct: 181 RLYIIAGVKNDTAFAPLTKKEISEIAWHRLDDLLPVSDDVISHGITGLKLYMVAPFLESL 240

Query: 241 RSWILTHQRPVVPNFDMPLKGVTMWKVKNSSMSSSSMVLDNQSSKSDSDPGKSFRNFKFD 300
           RSWIL HQ PV PNFDMP+KG TMWKVKN+SMS+SS++ DNQ  K DSDPG+S RNFKFD
Sbjct: 241 RSWILKHQPPVAPNFDMPVKGFTMWKVKNTSMSNSSIIFDNQPIKLDSDPGRSLRNFKFD 300

Query: 301 TASILQAMESAFAA 315
           TASIL AMESAFAA
Sbjct: 301 TASILHAMESAFAA 314

BLAST of MS004227 vs. NCBI nr
Match: XP_008446886.1 (PREDICTED: mRNA-decapping enzyme subunit 2-like [Cucumis melo] >KAA0034722.1 mRNA-decapping enzyme subunit 2-like [Cucumis melo var. makuwa] >TYK09275.1 mRNA-decapping enzyme subunit 2-like [Cucumis melo var. makuwa])

HSP 1 Score: 587.0 bits (1512), Expect = 9.4e-164
Identity = 286/314 (91.08%), Postives = 298/314 (94.90%), Query Frame = 0

Query: 1   MAGLHQPSTAPVKNGLPPQELLDDLCSRFVLNVPKEDLQSFERILFLVEYAHWFYEDNSV 60
           MA LH PS APVKNGLPPQELLDDLCSRFVLNVPKEDLQSFERILFLVEYAHWFYEDNSV
Sbjct: 1   MASLHLPSNAPVKNGLPPQELLDDLCSRFVLNVPKEDLQSFERILFLVEYAHWFYEDNSV 60

Query: 61  ENNPSLKSFNLKEFTSLLFNSCDVLKPYVPHIDDIFKDFTSYKLRVPVTGAIILDETFER 120
           ENNPSLKSFNLKEFTSLLF+SCDVLKPYVPHIDDIFKDFTSYKLRVPVTG IILDETFER
Sbjct: 61  ENNPSLKSFNLKEFTSLLFSSCDVLKPYVPHIDDIFKDFTSYKLRVPVTGGIILDETFER 120

Query: 121 CLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDVSAFLIKEDFIELMFGQQRV 180
           CLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDV+ FLIKEDFIE+MFGQQRV
Sbjct: 121 CLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDVTPFLIKEDFIEVMFGQQRV 180

Query: 181 RLYIIAGVKNDSSFAPLTKKEISEIAWHRLDDLLPASDDVISHGITGLKLYMVAPFLTSL 240
           RLYIIAGVKND++FAPLTKKEISEIAWHRLDDLLP SDDVISHGITGLKLYMVAPFL SL
Sbjct: 181 RLYIIAGVKNDTAFAPLTKKEISEIAWHRLDDLLPVSDDVISHGITGLKLYMVAPFLESL 240

Query: 241 RSWILTHQRPVVPNFDMPLKGVTMWKVKNSSMSSSSMVLDNQSSKSDSDPGKSFRNFKFD 300
           RSWIL HQ PV PNFDMP+KG TMWKVKN+SMS+SS++ DNQ  K DSDPG+S +NFKFD
Sbjct: 241 RSWILKHQPPVAPNFDMPVKGYTMWKVKNTSMSNSSIIFDNQPIKLDSDPGRSLKNFKFD 300

Query: 301 TASILQAMESAFAA 315
           TASILQAMESAFAA
Sbjct: 301 TASILQAMESAFAA 314

BLAST of MS004227 vs. NCBI nr
Match: XP_038891420.1 (mRNA-decapping enzyme subunit 2-like isoform X1 [Benincasa hispida])

HSP 1 Score: 578.6 bits (1490), Expect = 3.4e-161
Identity = 286/316 (90.51%), Postives = 296/316 (93.67%), Query Frame = 0

Query: 1   MAGLHQPSTAPVKNGLPPQELLDDLCSRFVLNVPKEDLQSFERILFLVEYAHWFYEDNSV 60
           MAGLH PS APVKNGLPPQELLDDLCSRFVLNVPKEDLQSFERILFLVEYAHWFYEDNSV
Sbjct: 1   MAGLHLPSNAPVKNGLPPQELLDDLCSRFVLNVPKEDLQSFERILFLVEYAHWFYEDNSV 60

Query: 61  ENNPSLKSFNLKEFTSLLFNS--CDVLKPYVPHIDDIFKDFTSYKLRVPVTGAIILDETF 120
           ENNPSLKSFNLKEFTSL   S  CDVLKPYVPHIDDIFKDFTSYKLRVPVTG IILDETF
Sbjct: 61  ENNPSLKSFNLKEFTSLCILSIICDVLKPYVPHIDDIFKDFTSYKLRVPVTGGIILDETF 120

Query: 121 ERCLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDVSAFLIKEDFIELMFGQQ 180
           ERCLLVKGWKGSSWSFPRGKKSKDEEDHACAIREV EETGFDV+AFLIKEDFIE+MFGQQ
Sbjct: 121 ERCLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVSEETGFDVTAFLIKEDFIEVMFGQQ 180

Query: 181 RVRLYIIAGVKNDSSFAPLTKKEISEIAWHRLDDLLPASDDVISHGITGLKLYMVAPFLT 240
           RVRLYIIAGVKND++FAPLTKKEISEIAWHRLDDLLP SDDVISHGITGLKLYMVAPFL 
Sbjct: 181 RVRLYIIAGVKNDTAFAPLTKKEISEIAWHRLDDLLPVSDDVISHGITGLKLYMVAPFLE 240

Query: 241 SLRSWILTHQRPVVPNFDMPLKGVTMWKVKNSSMSSSSMVLDNQSSKSDSDPGKSFRNFK 300
           SLRSWIL HQ PV PNFDMP+KGVT+WKVKNSSMS+SS++ D QS K DSDPG+S RNFK
Sbjct: 241 SLRSWILKHQPPVAPNFDMPVKGVTIWKVKNSSMSNSSIIFDGQSIKLDSDPGRSLRNFK 300

Query: 301 FDTASILQAMESAFAA 315
           FDTASIL AMESAFAA
Sbjct: 301 FDTASILHAMESAFAA 316

BLAST of MS004227 vs. ExPASy Swiss-Prot
Match: Q8GW31 (mRNA-decapping enzyme subunit 2 OS=Arabidopsis thaliana OX=3702 GN=DCP2 PE=1 SV=1)

HSP 1 Score: 431.0 bits (1107), Expect = 1.1e-119
Identity = 224/373 (60.05%), Postives = 265/373 (71.05%), Query Frame = 0

Query: 1   MAGLHQPSTAP--VKNGLPPQELLDDLCSRFVLNVPKEDLQSFERILFLVEYAHWFYEDN 60
           M+GLH+ S++   + N LP +ELLDDLCSRFVLNVP+ED QSFERILFLVEYA+W+YEDN
Sbjct: 1   MSGLHRSSSSSKNIGNCLPSKELLDDLCSRFVLNVPEEDQQSFERILFLVEYAYWYYEDN 60

Query: 61  SVENNPSLKSFNLKEFTSLLFNSCDVLKPYVPHIDDIFKDFTSYKLRVPVTGAIILDETF 120
           +VEN+P LKS +LKEFTSLLFNSCDVL+PYV HIDDIFKDFTSYK RVPVTGAIILDET+
Sbjct: 61  AVENDPKLKSLSLKEFTSLLFNSCDVLRPYVTHIDDIFKDFTSYKCRVPVTGAIILDETY 120

Query: 121 ERCLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDVSAFLIKEDFIELMFGQQ 180
           ERCLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDVS  L +E++IE +F QQ
Sbjct: 121 ERCLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDVSKLLKREEYIEFVFRQQ 180

Query: 181 RVRLYIIAGVKNDSSFAPLTKKEISEIAWHRLDDLLPASDDVISHGITGLKLYMVAPFLT 240
           RVRLYI+AGV  D+ FAPLTKKEISEI WHRLD L PAS++VI+HG++GLKLYMVAPFL+
Sbjct: 181 RVRLYIVAGVTEDTVFAPLTKKEISEITWHRLDHLQPASNEVITHGVSGLKLYMVAPFLS 240

Query: 241 SLRSWILTHQRPVVPNFDMPLKGVTMWKVKNSSMSSSSMVLDNQSSKS------------ 300
           SL+SWIL H  PV    + PLK + +W  + S   + +  +++Q+ KS            
Sbjct: 241 SLKSWILKHPSPVARRPNKPLKALCVWNARTSVGGNGTATVESQNRKSELRTTTMESNSR 300

Query: 301 ---------------------------------------------DSDPGKSFRNFKFDT 315
                                                        D  PG SF NFKF+ 
Sbjct: 301 KPELKRTTMESHSTKPELRKGTMESHNTTATVESHNTKPVVDHSQDIKPGGSFINFKFNQ 360

BLAST of MS004227 vs. ExPASy Swiss-Prot
Match: O13828 (mRNA decapping complex subunit 2 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=dcp2 PE=1 SV=1)

HSP 1 Score: 210.7 bits (535), Expect = 2.4e-53
Identity = 108/226 (47.79%), Postives = 141/226 (62.39%), Query Frame = 0

Query: 20  ELLDDLCSRFVLNVPKEDLQSFERILFLVEYAHWFYEDNSVENNPSLKSFNLKEFTSLLF 79
           ++LDDL +RF+LN+P E+  S ER+ F +E AHWFYED     N  L S  L+ F++ LF
Sbjct: 10  QVLDDLSARFILNLPAEEQSSVERLCFQIEQAHWFYEDFIRAQNDQLPSLGLRVFSAKLF 69

Query: 80  NSCDVLKPYVPHIDDIFKDFTSYKLRVPVTGAIILDETFERCLLVKGWKGSS-WSFPRGK 139
             C +L  +    ++ F DF  YK R+PV GAI+LD + ++C+LVKGWK SS W FP+GK
Sbjct: 70  AHCPLLWKWSKVHEEAFDDFLRYKTRIPVRGAIMLDMSMQQCVLVKGWKASSGWGFPKGK 129

Query: 140 KSKDEEDHACAIREVLEETGFDVSAFLIKEDFIELMFGQQRVRLYIIAGVKNDSSFAPLT 199
             KDE D  CAIREV EETGFD S+ +   +FI++    Q VRLYII G+  D+ F   T
Sbjct: 130 IDKDESDVDCAIREVYEETGFDCSSRINPNEFIDMTIRGQNVRLYIIPGISLDTRFESRT 189

Query: 200 KKEISEIAWHRLDDLLPASDDVISHGITGLKLYMVAPFLTSLRSWI 245
           +KEIS+I WH L DL     +         K YMV PFL  L+ WI
Sbjct: 190 RKEISKIEWHNLMDLPTFKKN--KPQTMKNKFYMVIPFLAPLKKWI 233

BLAST of MS004227 vs. ExPASy Swiss-Prot
Match: Q9CYC6 (m7GpppN-mRNA hydrolase OS=Mus musculus OX=10090 GN=Dcp2 PE=1 SV=2)

HSP 1 Score: 206.8 bits (525), Expect = 3.5e-52
Identity = 99/231 (42.86%), Postives = 139/231 (60.17%), Query Frame = 0

Query: 18  PQELLDDLCSRFVLNVPKEDLQSFERILFLVEYAHWFYEDNSVENNPSLKSFNLKEFTSL 77
           P  +LDDLCSRF+L++P E+  +  R+ F +E AHWFY D  ++N P L    +++F   
Sbjct: 9   PGSVLDDLCSRFILHIPSEERDNAIRVCFQIELAHWFYLDFYMQNTPGLPQCGIRDFAKA 68

Query: 78  LFNSCDVLKPYVPHIDDIFKDFTSYKLRVPVTGAIILDETFERCLLVKGWKG-SSWSFPR 137
           +F+ C  L P    ++ I  ++  YK+ VP  GAIILDET E  LLV+G+   S W FP+
Sbjct: 69  VFSHCPFLLPQGEDVEKILDEWKEYKMGVPTYGAIILDETLENVLLVQGYLAKSGWGFPK 128

Query: 138 GKKSKDEEDHACAIREVLEETGFDVSAFLIKEDFIELMFGQQRVRLYIIAGVKNDSSFAP 197
           GK +K+E  H CA REV EETGFD+  ++ K+D+IEL    Q  RLYII GV  D+ F P
Sbjct: 129 GKVNKEEAPHDCAAREVFEETGFDIKDYICKDDYIELRINDQLARLYIIPGVPKDTKFNP 188

Query: 198 LTKKEISEIAWHRLDDLLPASDDVISHGITGL---KLYMVAPFLTSLRSWI 245
            T++EI  I W  ++ L    +D+      GL   K +M  PF+  LR W+
Sbjct: 189 KTRREIRNIEWFSIEKLPCHRNDMTPKSKLGLAPNKFFMAIPFIRPLRDWL 239

BLAST of MS004227 vs. ExPASy Swiss-Prot
Match: Q8IU60 (m7GpppN-mRNA hydrolase OS=Homo sapiens OX=9606 GN=DCP2 PE=1 SV=3)

HSP 1 Score: 206.1 bits (523), Expect = 5.9e-52
Identity = 97/231 (41.99%), Postives = 139/231 (60.17%), Query Frame = 0

Query: 18  PQELLDDLCSRFVLNVPKEDLQSFERILFLVEYAHWFYEDNSVENNPSLKSFNLKEFTSL 77
           P  +LDDLCSRF+L++P E+  +  R+ F +E AHWFY D  ++N P L    +++F   
Sbjct: 9   PGSVLDDLCSRFILHIPSEERDNAIRVCFQIELAHWFYLDFYMQNTPGLPQCGIRDFAKA 68

Query: 78  LFNSCDVLKPYVPHIDDIFKDFTSYKLRVPVTGAIILDETFERCLLVKGWKG-SSWSFPR 137
           +F+ C  L P    ++ +  ++  YK+ VP  GAIILDET E  LLV+G+   S W FP+
Sbjct: 69  VFSHCPFLLPQGEDVEKVLDEWKEYKMGVPTYGAIILDETLENVLLVQGYLAKSGWGFPK 128

Query: 138 GKKSKDEEDHACAIREVLEETGFDVSAFLIKEDFIELMFGQQRVRLYIIAGVKNDSSFAP 197
           GK +K+E  H CA REV EETGFD+  ++ K+D+IEL    Q  RLYII G+  D+ F P
Sbjct: 129 GKVNKEEAPHDCAAREVFEETGFDIKDYICKDDYIELRINDQLARLYIIPGIPKDTKFNP 188

Query: 198 LTKKEISEIAWHRLDDLLPASDDVISHGITGL---KLYMVAPFLTSLRSWI 245
            T++EI  I W  ++ L    +D+      GL   K +M  PF+  LR W+
Sbjct: 189 KTRREIRNIEWFSIEKLPCHRNDMTPKSKLGLAPNKFFMAIPFIRPLRDWL 239

BLAST of MS004227 vs. ExPASy Swiss-Prot
Match: Q5REQ8 (m7GpppN-mRNA hydrolase OS=Pongo abelii OX=9601 GN=DCP2 PE=2 SV=1)

HSP 1 Score: 206.1 bits (523), Expect = 5.9e-52
Identity = 97/231 (41.99%), Postives = 139/231 (60.17%), Query Frame = 0

Query: 18  PQELLDDLCSRFVLNVPKEDLQSFERILFLVEYAHWFYEDNSVENNPSLKSFNLKEFTSL 77
           P  +LDDLCSRF+L++P E+  +  R+ F +E AHWFY D  ++N P L    +++F   
Sbjct: 9   PGSVLDDLCSRFILHIPSEERDNAIRVCFQIELAHWFYLDFYMQNTPGLSQCGIRDFAKA 68

Query: 78  LFNSCDVLKPYVPHIDDIFKDFTSYKLRVPVTGAIILDETFERCLLVKGWKG-SSWSFPR 137
           +F+ C  L P    ++ +  ++  YK+ VP  GAIILDET E  LLV+G+   S W FP+
Sbjct: 69  VFSHCPFLLPEGEDVEKVLDEWKEYKMGVPTYGAIILDETLENVLLVQGYLAKSGWGFPK 128

Query: 138 GKKSKDEEDHACAIREVLEETGFDVSAFLIKEDFIELMFGQQRVRLYIIAGVKNDSSFAP 197
           GK +K+E  H CA REV EETGFD+  ++ K+D+IEL    Q  RLYII G+  D+ F P
Sbjct: 129 GKVNKEEAPHDCAAREVFEETGFDIKDYICKDDYIELRINDQLARLYIIPGIPKDTKFNP 188

Query: 198 LTKKEISEIAWHRLDDLLPASDDVISHGITGL---KLYMVAPFLTSLRSWI 245
            T++EI  I W  ++ L    +D+      GL   K +M  PF+  LR W+
Sbjct: 189 KTRREIRNIEWFSIEKLPCHRNDMTPKSKLGLAPNKFFMAIPFIRPLRDWL 239

BLAST of MS004227 vs. ExPASy TrEMBL
Match: A0A6J1CC63 (mRNA-decapping enzyme subunit 2-like OS=Momordica charantia OX=3673 GN=LOC111010222 PE=3 SV=1)

HSP 1 Score: 630.6 bits (1625), Expect = 3.6e-177
Identity = 312/314 (99.36%), Postives = 312/314 (99.36%), Query Frame = 0

Query: 1   MAGLHQPSTAPVKNGLPPQELLDDLCSRFVLNVPKEDLQSFERILFLVEYAHWFYEDNSV 60
           MAGLHQPS APVKNGLPPQELLDDLCSRFVLNVPKEDLQSFERILFLVEYAHWFYEDNSV
Sbjct: 1   MAGLHQPSIAPVKNGLPPQELLDDLCSRFVLNVPKEDLQSFERILFLVEYAHWFYEDNSV 60

Query: 61  ENNPSLKSFNLKEFTSLLFNSCDVLKPYVPHIDDIFKDFTSYKLRVPVTGAIILDETFER 120
           ENNPSLKSFNLKEFTSLLFNSCDVLKPYVPHIDDIFKDFTSYKLRVPVTGAIILDETFER
Sbjct: 61  ENNPSLKSFNLKEFTSLLFNSCDVLKPYVPHIDDIFKDFTSYKLRVPVTGAIILDETFER 120

Query: 121 CLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDVSAFLIKEDFIELMFGQQRV 180
           CLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDVSAFLIKEDFIELMFGQQRV
Sbjct: 121 CLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDVSAFLIKEDFIELMFGQQRV 180

Query: 181 RLYIIAGVKNDSSFAPLTKKEISEIAWHRLDDLLPASDDVISHGITGLKLYMVAPFLTSL 240
           RLYIIAGVKNDSSFAPLTKKEISEIAWHRLDDLLPASDDVISHGITGLKLYMVAPFLTSL
Sbjct: 181 RLYIIAGVKNDSSFAPLTKKEISEIAWHRLDDLLPASDDVISHGITGLKLYMVAPFLTSL 240

Query: 241 RSWILTHQRPVVPNFDMPLKGVTMWKVKNSSMSSSSMVLDNQSSKSDSDPGKSFRNFKFD 300
           RSWILTHQRPVVPNFDMPLKGVTMWKVKNSSMSSSSMVLDNQSSKSDSDPGKSFRNFKFD
Sbjct: 241 RSWILTHQRPVVPNFDMPLKGVTMWKVKNSSMSSSSMVLDNQSSKSDSDPGKSFRNFKFD 300

Query: 301 TASILQAMESAFAA 315
           TASILQAME AFAA
Sbjct: 301 TASILQAMEGAFAA 314

BLAST of MS004227 vs. ExPASy TrEMBL
Match: A0A5D3CCQ6 (mRNA-decapping enzyme subunit 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold475G002700 PE=3 SV=1)

HSP 1 Score: 587.0 bits (1512), Expect = 4.6e-164
Identity = 286/314 (91.08%), Postives = 298/314 (94.90%), Query Frame = 0

Query: 1   MAGLHQPSTAPVKNGLPPQELLDDLCSRFVLNVPKEDLQSFERILFLVEYAHWFYEDNSV 60
           MA LH PS APVKNGLPPQELLDDLCSRFVLNVPKEDLQSFERILFLVEYAHWFYEDNSV
Sbjct: 1   MASLHLPSNAPVKNGLPPQELLDDLCSRFVLNVPKEDLQSFERILFLVEYAHWFYEDNSV 60

Query: 61  ENNPSLKSFNLKEFTSLLFNSCDVLKPYVPHIDDIFKDFTSYKLRVPVTGAIILDETFER 120
           ENNPSLKSFNLKEFTSLLF+SCDVLKPYVPHIDDIFKDFTSYKLRVPVTG IILDETFER
Sbjct: 61  ENNPSLKSFNLKEFTSLLFSSCDVLKPYVPHIDDIFKDFTSYKLRVPVTGGIILDETFER 120

Query: 121 CLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDVSAFLIKEDFIELMFGQQRV 180
           CLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDV+ FLIKEDFIE+MFGQQRV
Sbjct: 121 CLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDVTPFLIKEDFIEVMFGQQRV 180

Query: 181 RLYIIAGVKNDSSFAPLTKKEISEIAWHRLDDLLPASDDVISHGITGLKLYMVAPFLTSL 240
           RLYIIAGVKND++FAPLTKKEISEIAWHRLDDLLP SDDVISHGITGLKLYMVAPFL SL
Sbjct: 181 RLYIIAGVKNDTAFAPLTKKEISEIAWHRLDDLLPVSDDVISHGITGLKLYMVAPFLESL 240

Query: 241 RSWILTHQRPVVPNFDMPLKGVTMWKVKNSSMSSSSMVLDNQSSKSDSDPGKSFRNFKFD 300
           RSWIL HQ PV PNFDMP+KG TMWKVKN+SMS+SS++ DNQ  K DSDPG+S +NFKFD
Sbjct: 241 RSWILKHQPPVAPNFDMPVKGYTMWKVKNTSMSNSSIIFDNQPIKLDSDPGRSLKNFKFD 300

Query: 301 TASILQAMESAFAA 315
           TASILQAMESAFAA
Sbjct: 301 TASILQAMESAFAA 314

BLAST of MS004227 vs. ExPASy TrEMBL
Match: A0A0A0KWT4 (Nudix hydrolase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G622860 PE=3 SV=1)

HSP 1 Score: 587.0 bits (1512), Expect = 4.6e-164
Identity = 287/314 (91.40%), Postives = 297/314 (94.59%), Query Frame = 0

Query: 1   MAGLHQPSTAPVKNGLPPQELLDDLCSRFVLNVPKEDLQSFERILFLVEYAHWFYEDNSV 60
           MAGLH PS A VKNGLPPQELLDDLCSRFVLNVPKEDLQSFERILFLVEYAHWFYEDNSV
Sbjct: 1   MAGLHLPSNAHVKNGLPPQELLDDLCSRFVLNVPKEDLQSFERILFLVEYAHWFYEDNSV 60

Query: 61  ENNPSLKSFNLKEFTSLLFNSCDVLKPYVPHIDDIFKDFTSYKLRVPVTGAIILDETFER 120
           ENNPSLKSFNLKEFTSLLFNSCDVLKPYVPHIDDIFKDFTSYKLRVPVTG IILDETFER
Sbjct: 61  ENNPSLKSFNLKEFTSLLFNSCDVLKPYVPHIDDIFKDFTSYKLRVPVTGGIILDETFER 120

Query: 121 CLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDVSAFLIKEDFIELMFGQQRV 180
           CLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDV+ FLIKEDFIE+MFGQQRV
Sbjct: 121 CLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDVTPFLIKEDFIEVMFGQQRV 180

Query: 181 RLYIIAGVKNDSSFAPLTKKEISEIAWHRLDDLLPASDDVISHGITGLKLYMVAPFLTSL 240
           RLYIIAGVKND++FAPLTKKEISEIAWHRLDDLLP SDDVISHGITGLKLYMVAPFL SL
Sbjct: 181 RLYIIAGVKNDTAFAPLTKKEISEIAWHRLDDLLPVSDDVISHGITGLKLYMVAPFLESL 240

Query: 241 RSWILTHQRPVVPNFDMPLKGVTMWKVKNSSMSSSSMVLDNQSSKSDSDPGKSFRNFKFD 300
           RSWIL HQ PV PNFDMP+KG TMWKVKN+SMS+SS++ DNQ  K DSDPG+S RNFKFD
Sbjct: 241 RSWILKHQPPVAPNFDMPVKGFTMWKVKNTSMSNSSIIFDNQPIKLDSDPGRSLRNFKFD 300

Query: 301 TASILQAMESAFAA 315
           TASIL AMESAFAA
Sbjct: 301 TASILHAMESAFAA 314

BLAST of MS004227 vs. ExPASy TrEMBL
Match: A0A1S3BG59 (mRNA-decapping enzyme subunit 2-like OS=Cucumis melo OX=3656 GN=LOC103489466 PE=3 SV=1)

HSP 1 Score: 587.0 bits (1512), Expect = 4.6e-164
Identity = 286/314 (91.08%), Postives = 298/314 (94.90%), Query Frame = 0

Query: 1   MAGLHQPSTAPVKNGLPPQELLDDLCSRFVLNVPKEDLQSFERILFLVEYAHWFYEDNSV 60
           MA LH PS APVKNGLPPQELLDDLCSRFVLNVPKEDLQSFERILFLVEYAHWFYEDNSV
Sbjct: 1   MASLHLPSNAPVKNGLPPQELLDDLCSRFVLNVPKEDLQSFERILFLVEYAHWFYEDNSV 60

Query: 61  ENNPSLKSFNLKEFTSLLFNSCDVLKPYVPHIDDIFKDFTSYKLRVPVTGAIILDETFER 120
           ENNPSLKSFNLKEFTSLLF+SCDVLKPYVPHIDDIFKDFTSYKLRVPVTG IILDETFER
Sbjct: 61  ENNPSLKSFNLKEFTSLLFSSCDVLKPYVPHIDDIFKDFTSYKLRVPVTGGIILDETFER 120

Query: 121 CLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDVSAFLIKEDFIELMFGQQRV 180
           CLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDV+ FLIKEDFIE+MFGQQRV
Sbjct: 121 CLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDVTPFLIKEDFIEVMFGQQRV 180

Query: 181 RLYIIAGVKNDSSFAPLTKKEISEIAWHRLDDLLPASDDVISHGITGLKLYMVAPFLTSL 240
           RLYIIAGVKND++FAPLTKKEISEIAWHRLDDLLP SDDVISHGITGLKLYMVAPFL SL
Sbjct: 181 RLYIIAGVKNDTAFAPLTKKEISEIAWHRLDDLLPVSDDVISHGITGLKLYMVAPFLESL 240

Query: 241 RSWILTHQRPVVPNFDMPLKGVTMWKVKNSSMSSSSMVLDNQSSKSDSDPGKSFRNFKFD 300
           RSWIL HQ PV PNFDMP+KG TMWKVKN+SMS+SS++ DNQ  K DSDPG+S +NFKFD
Sbjct: 241 RSWILKHQPPVAPNFDMPVKGYTMWKVKNTSMSNSSIIFDNQPIKLDSDPGRSLKNFKFD 300

Query: 301 TASILQAMESAFAA 315
           TASILQAMESAFAA
Sbjct: 301 TASILQAMESAFAA 314

BLAST of MS004227 vs. ExPASy TrEMBL
Match: A0A6J1IK27 (mRNA-decapping enzyme subunit 2-like OS=Cucurbita maxima OX=3661 GN=LOC111477819 PE=3 SV=1)

HSP 1 Score: 570.1 bits (1468), Expect = 5.8e-159
Identity = 281/314 (89.49%), Postives = 292/314 (92.99%), Query Frame = 0

Query: 1   MAGLHQPSTAPVKNGLPPQELLDDLCSRFVLNVPKEDLQSFERILFLVEYAHWFYEDNSV 60
           MAG+HQP+ A VKNGLPPQELLDDLCSRFVLNVP+EDLQSFERILFLVEYAHWFYEDNSV
Sbjct: 1   MAGIHQPANASVKNGLPPQELLDDLCSRFVLNVPREDLQSFERILFLVEYAHWFYEDNSV 60

Query: 61  ENNPSLKSFNLKEFTSLLFNSCDVLKPYVPHIDDIFKDFTSYKLRVPVTGAIILDETFER 120
           ENNPSLKSFNLKEFTSLLFNSCDVLKPYVPHIDDIFKDFTSYKLRVPVTG IILDETFER
Sbjct: 61  ENNPSLKSFNLKEFTSLLFNSCDVLKPYVPHIDDIFKDFTSYKLRVPVTGGIILDETFER 120

Query: 121 CLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDVSAFLIKEDFIELMFGQQRV 180
           CLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDV+ FLI++DF+E+MFGQQRV
Sbjct: 121 CLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDVTPFLIEDDFLEVMFGQQRV 180

Query: 181 RLYIIAGVKNDSSFAPLTKKEISEIAWHRLDDLLPASDDVISHGITGLKLYMVAPFLTSL 240
           RLYIIAGVK D  FAPLTKKEISEIAWHRLDDLLPASDDVISHGITGLKLYMVAPFL SL
Sbjct: 181 RLYIIAGVKTDIPFAPLTKKEISEIAWHRLDDLLPASDDVISHGITGLKLYMVAPFLNSL 240

Query: 241 RSWILTHQRPVVPNFDMPLKGVTMWKVKNSSMSSSSMVLDNQSSKSDSDPGKSFRNFKFD 300
           RSWIL HQ PV PNFDM +KGVTMWKVKN     SS+V D Q +K DSDPGKS RNFKFD
Sbjct: 241 RSWILKHQPPVNPNFDMHVKGVTMWKVKN-----SSIVFDGQQTKFDSDPGKSLRNFKFD 300

Query: 301 TASILQAMESAFAA 315
           TASILQAMESAFAA
Sbjct: 301 TASILQAMESAFAA 309

BLAST of MS004227 vs. TAIR 10
Match: AT5G13570.1 (decapping 2 )

HSP 1 Score: 431.0 bits (1107), Expect = 8.1e-121
Identity = 224/373 (60.05%), Postives = 265/373 (71.05%), Query Frame = 0

Query: 1   MAGLHQPSTAP--VKNGLPPQELLDDLCSRFVLNVPKEDLQSFERILFLVEYAHWFYEDN 60
           M+GLH+ S++   + N LP +ELLDDLCSRFVLNVP+ED QSFERILFLVEYA+W+YEDN
Sbjct: 1   MSGLHRSSSSSKNIGNCLPSKELLDDLCSRFVLNVPEEDQQSFERILFLVEYAYWYYEDN 60

Query: 61  SVENNPSLKSFNLKEFTSLLFNSCDVLKPYVPHIDDIFKDFTSYKLRVPVTGAIILDETF 120
           +VEN+P LKS +LKEFTSLLFNSCDVL+PYV HIDDIFKDFTSYK RVPVTGAIILDET+
Sbjct: 61  AVENDPKLKSLSLKEFTSLLFNSCDVLRPYVTHIDDIFKDFTSYKCRVPVTGAIILDETY 120

Query: 121 ERCLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDVSAFLIKEDFIELMFGQQ 180
           ERCLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDVS  L +E++IE +F QQ
Sbjct: 121 ERCLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDVSKLLKREEYIEFVFRQQ 180

Query: 181 RVRLYIIAGVKNDSSFAPLTKKEISEIAWHRLDDLLPASDDVISHGITGLKLYMVAPFLT 240
           RVRLYI+AGV  D+ FAPLTKKEISEI WHRLD L PAS++VI+HG++GLKLYMVAPFL+
Sbjct: 181 RVRLYIVAGVTEDTVFAPLTKKEISEITWHRLDHLQPASNEVITHGVSGLKLYMVAPFLS 240

Query: 241 SLRSWILTHQRPVVPNFDMPLKGVTMWKVKNSSMSSSSMVLDNQSSKS------------ 300
           SL+SWIL H  PV    + PLK + +W  + S   + +  +++Q+ KS            
Sbjct: 241 SLKSWILKHPSPVARRPNKPLKALCVWNARTSVGGNGTATVESQNRKSELRTTTMESNSR 300

Query: 301 ---------------------------------------------DSDPGKSFRNFKFDT 315
                                                        D  PG SF NFKF+ 
Sbjct: 301 KPELKRTTMESHSTKPELRKGTMESHNTTATVESHNTKPVVDHSQDIKPGGSFINFKFNQ 360

BLAST of MS004227 vs. TAIR 10
Match: AT5G13570.2 (decapping 2 )

HSP 1 Score: 421.8 bits (1083), Expect = 4.9e-118
Identity = 224/386 (58.03%), Postives = 265/386 (68.65%), Query Frame = 0

Query: 1   MAGLHQPSTAP--VKNGLPPQELLDDLCSRFVLNVPKEDLQSFERILFLVEYAHWFYEDN 60
           M+GLH+ S++   + N LP +ELLDDLCSRFVLNVP+ED QSFERILFLVEYA+W+YEDN
Sbjct: 1   MSGLHRSSSSSKNIGNCLPSKELLDDLCSRFVLNVPEEDQQSFERILFLVEYAYWYYEDN 60

Query: 61  SVENNPSLKSFNLKEFTSLLFNSCDVLKPYVPHIDDIFKDFTSYKLRVPVTGAIILDETF 120
           +VEN+P LKS +LKEFTSLLFNSCDVL+PYV HIDDIFKDFTSYK RVPVTGAIILDET+
Sbjct: 61  AVENDPKLKSLSLKEFTSLLFNSCDVLRPYVTHIDDIFKDFTSYKCRVPVTGAIILDETY 120

Query: 121 ERCLLVKGWKGSSWSFPRGKKSKDEEDHACAIRE-------------VLEETGFDVSAFL 180
           ERCLLVKGWKGSSWSFPRGKKSKDEEDHACAIRE             VLEETGFDVS  L
Sbjct: 121 ERCLLVKGWKGSSWSFPRGKKSKDEEDHACAIRELSSAILLVNVAFQVLEETGFDVSKLL 180

Query: 181 IKEDFIELMFGQQRVRLYIIAGVKNDSSFAPLTKKEISEIAWHRLDDLLPASDDVISHGI 240
            +E++IE +F QQRVRLYI+AGV  D+ FAPLTKKEISEI WHRLD L PAS++VI+HG+
Sbjct: 181 KREEYIEFVFRQQRVRLYIVAGVTEDTVFAPLTKKEISEITWHRLDHLQPASNEVITHGV 240

Query: 241 TGLKLYMVAPFLTSLRSWILTHQRPVVPNFDMPLKGVTMWKVKNSSMSSSSMVLDNQSSK 300
           +GLKLYMVAPFL+SL+SWIL H  PV    + PLK + +W  + S   + +  +++Q+ K
Sbjct: 241 SGLKLYMVAPFLSSLKSWILKHPSPVARRPNKPLKALCVWNARTSVGGNGTATVESQNRK 300

Query: 301 S---------------------------------------------------------DS 315
           S                                                         D 
Sbjct: 301 SELRTTTMESNSRKPELKRTTMESHSTKPELRKGTMESHNTTATVESHNTKPVVDHSQDI 360

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022139266.17.4e-17799.36mRNA-decapping enzyme subunit 2-like [Momordica charantia][more]
XP_038891421.15.0e-16592.04mRNA-decapping enzyme subunit 2-like isoform X2 [Benincasa hispida][more]
XP_004142410.19.4e-16491.40mRNA-decapping enzyme subunit 2 [Cucumis sativus] >XP_031742317.1 mRNA-decapping... [more]
XP_008446886.19.4e-16491.08PREDICTED: mRNA-decapping enzyme subunit 2-like [Cucumis melo] >KAA0034722.1 mRN... [more]
XP_038891420.13.4e-16190.51mRNA-decapping enzyme subunit 2-like isoform X1 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
Q8GW311.1e-11960.05mRNA-decapping enzyme subunit 2 OS=Arabidopsis thaliana OX=3702 GN=DCP2 PE=1 SV=... [more]
O138282.4e-5347.79mRNA decapping complex subunit 2 OS=Schizosaccharomyces pombe (strain 972 / ATCC... [more]
Q9CYC63.5e-5242.86m7GpppN-mRNA hydrolase OS=Mus musculus OX=10090 GN=Dcp2 PE=1 SV=2[more]
Q8IU605.9e-5241.99m7GpppN-mRNA hydrolase OS=Homo sapiens OX=9606 GN=DCP2 PE=1 SV=3[more]
Q5REQ85.9e-5241.99m7GpppN-mRNA hydrolase OS=Pongo abelii OX=9601 GN=DCP2 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1CC633.6e-17799.36mRNA-decapping enzyme subunit 2-like OS=Momordica charantia OX=3673 GN=LOC111010... [more]
A0A5D3CCQ64.6e-16491.08mRNA-decapping enzyme subunit 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E... [more]
A0A0A0KWT44.6e-16491.40Nudix hydrolase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G62... [more]
A0A1S3BG594.6e-16491.08mRNA-decapping enzyme subunit 2-like OS=Cucumis melo OX=3656 GN=LOC103489466 PE=... [more]
A0A6J1IK275.8e-15989.49mRNA-decapping enzyme subunit 2-like OS=Cucurbita maxima OX=3661 GN=LOC111477819... [more]
Match NameE-valueIdentityDescription
AT5G13570.18.1e-12160.05decapping 2 [more]
AT5G13570.24.9e-11858.03decapping 2 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR007722mRNA decapping protein 2, Box A domainSMARTSM01125DCP2_2coord: 19..103
e-value: 2.9E-43
score: 159.7
IPR007722mRNA decapping protein 2, Box A domainPFAMPF05026DCP2coord: 20..102
e-value: 2.1E-28
score: 98.4
NoneNo IPR availableGENE3D3.90.79.10Nucleoside Triphosphate Pyrophosphohydrolasecoord: 105..245
e-value: 4.1E-86
score: 289.8
NoneNo IPR availablePANTHERPTHR23114M7GPPPN-MRNA HYDROLASEcoord: 13..261
IPR000086NUDIX hydrolase domainPFAMPF00293NUDIXcoord: 106..217
e-value: 3.6E-13
score: 49.7
IPR000086NUDIX hydrolase domainPROSITEPS51462NUDIXcoord: 104..233
score: 13.435682
IPR036189mRNA decapping protein 2, Box A domain superfamilyGENE3D1.10.10.1050Dcp2, box A domaincoord: 21..104
e-value: 4.1E-86
score: 289.8
IPR036189mRNA decapping protein 2, Box A domain superfamilySUPERFAMILY140586Dcp2 domain-likecoord: 14..103
IPR020084NUDIX hydrolase, conserved sitePROSITEPS00893NUDIX_BOXcoord: 137..158
IPR044099mRNA decapping enzyme 2 , NUDIX hydrolase domainCDDcd03672Dcp2pcoord: 106..250
e-value: 9.18007E-73
score: 219.417
IPR015797NUDIX hydrolase-like domain superfamilySUPERFAMILY55811Nudixcoord: 106..247

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS004227.1MS004227.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0000290 deadenylation-dependent decapping of nuclear-transcribed mRNA
biological_process GO:0000184 nuclear-transcribed mRNA catabolic process, nonsense-mediated decay
molecular_function GO:0016787 hydrolase activity
molecular_function GO:0050072 m7G(5')pppN diphosphatase activity
molecular_function GO:0030145 manganese ion binding
molecular_function GO:0003723 RNA binding