MS004153 (gene) Bitter gourd (TR) v1

Overview


Name	MS004153
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	iron-sulfur assembly protein IscA-like 1, mitochondrial
Location	scaffold92: 359774 .. 361750 (+)
RNA-Seq Expression	MS004153
Synteny	MS004153

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCAGCTTCTTCGTCTTCCTTTTTCGCCGCAGCTTCCAAGAAGGTTGGGCCAGCCGCACGCCGCCAAGTTCTTACTCTTACGGAGGCCGCTGCCGAAAGAATTCGACAACTTCTGCAACAGCGCCAACGTCCTTTCCTTCGCCTCGGCGTCAAGGCGCGCGGCTGCAATGGCTTATCCTATACTCTCAACTATGCAGGTTCGTAATATCTATTGGATTTCACCCTTTTCTTGATTCTATGCGTTTCTTTTTTACTCATTTTAGGTATGAATTTAAGGATATGACGAAATTTGTCAAGCAGCTATTCATCGTCTTTTCTCAGAATCAGATGTGGCTGCTTAGAGTTAATTCGAGACAATTAGGTATTTTATGTATTCGGCCGATGTAACCTAAATATTTTAACTAGAGAAACTGGTTCATCTGTTTACATCTCCAATTAATACCGAGAATAAATATTGCAAGACCAAGGATTTCTTAAAGATGATTAGATTAGATGTAGAAAAATTGGAAGAATAAATGATTATGTGATTATGATTTACTGGAATTTGGCCTGGGCATGGTATGATGTTCGAAGATGTGCTGTTGAATTCTTGCTAACCTTAACAAACACAAATCTAAAAGCTAAAACTGACATGCCTTAATGCGAAAGTCGGTAGATTGGATATATTTTTACGTGCAGGGACCTATATATGAGAGGAGTATTAACTAATAGTTCAGTCAGTAAATCATTCTGGATCTTAGAGTCAGTTATGAATTAATGGCGTTATTTGATAGGAAGTTTTAAATTAACGATTTATTTGGAAAACTGGTGAGATGGGGATTATTGAAACACAAAGTGCTGTTTTTGTTCAATTAGAAGATAATTGGGTTAGCAAGCCTCCGTGGGTCAACTTGGAGAGAATTGTTATCAATATGTCAGATGGTCAGAAGTTAGAGATTTTAAACTTGTGGATACGGACATTTTCTTGTTTATGTGTTGATTTAATGCGGCGATGTTCCCAGTTAACTGTGATTGGAGGCCTTTCCTAACTCAGCATTACCTCAGTTTTTTATTCACATTATCATTAATTTCTAGCCTTCTCCATGGTGCAAAAAGATGATGCAGCCTTTATCTCTTTTTTGGCATAAAGGGACTGTAGTCATGATCTCTATCTTTTGAACTACTTCAAAATTGCACTTTTTCTCATATTGAAGTTTTCTTATGTCAAATATTGCAGATGAGAAAGGGAAGTTTGATGAACTGGTTGAGGATCAAGACGTGAAGATATTGATCGAGCCAAAGGCTCTGATGCATGTAATAGGAACAAAGATGGACTTTGTAGATGACAAACTGAGGTATTTCTCTTCCACAATTTTATCTTCAGCTGCATGTCCCTCCATGTTGAGGAAATACCCTGCTTGTTAGTTGAGATATTAGAAAGATATATCAAAGCATATGTAATTGCATTTAAACATCAATAAGTAGGCATCAGTATGTAAACCTATTATTCCGCATCTATCATGGCTCCTTGGAAACACTGCTTTGATATTCCTGATTTTCAGACATGATTTAAGATGTTTTATTCGGCTCTGAGCAATTCCATTCTGAGTTTGCTTGTCAATCAATTTGCAATAGTGGCAGTGGAATTTGCAACTTGCAAGGATCATATTAGTCATTTGCATCTATATAAATGGGAGTATCTGTGCTTGAGAATGGATTCAGATCATCTTTAGCATCCTCGAGCTGGGGAATATATTTAATACTACTTAAGAGAGAACTATTAAGTTTATTAGAGTTAGGTTATCTCGAGTCTTGTTGCTCAAATCAAATTCATCTTTTTAATCTCTGTACTGCTACGTCCGGTGGGCAATGCACTCATTTCTTTTGTTCCTCCCTTGTTAGATCCGAGTTTATCTTTATCAATCCAAATTCCAAAGGACAATGTGGCTGTGGGGAGTCCTTTATGACAACAGGCAGTGCAGAAGCCAACAAAAAG

mRNA sequence

ATGGCAGCTTCTTCGTCTTCCTTTTTCGCCGCAGCTTCCAAGAAGGTTGGGCCAGCCGCACGCCGCCAAGTTCTTACTCTTACGGAGGCCGCTGCCGAAAGAATTCGACAACTTCTGCAACAGCGCCAACGTCCTTTCCTTCGCCTCGGCGTCAAGGCGCGCGGCTGCAATGGCTTATCCTATACTCTCAACTATGCAGATGAGAAAGGGAAGTTTGATGAACTGGTTGAGGATCAAGACGTGAAGATATTGATCGAGCCAAAGGCTCTGATGCATGTAATAGGAACAAAGATGGACTTTGTAGATGACAAACTGAGATCCGAGTTTATCTTTATCAATCCAAATTCCAAAGGACAATGTGGCTGTGGGGAGTCCTTTATGACAACAGGCAGTGCAGAAGCCAACAAAAAG

Coding sequence (CDS)

ATGGCAGCTTCTTCGTCTTCCTTTTTCGCCGCAGCTTCCAAGAAGGTTGGGCCAGCCGCACGCCGCCAAGTTCTTACTCTTACGGAGGCCGCTGCCGAAAGAATTCGACAACTTCTGCAACAGCGCCAACGTCCTTTCCTTCGCCTCGGCGTCAAGGCGCGCGGCTGCAATGGCTTATCCTATACTCTCAACTATGCAGATGAGAAAGGGAAGTTTGATGAACTGGTTGAGGATCAAGACGTGAAGATATTGATCGAGCCAAAGGCTCTGATGCATGTAATAGGAACAAAGATGGACTTTGTAGATGACAAACTGAGATCCGAGTTTATCTTTATCAATCCAAATTCCAAAGGACAATGTGGCTGTGGGGAGTCCTTTATGACAACAGGCAGTGCAGAAGCCAACAAAAAG

Protein sequence

MAASSSSFFAAASKKVGPAARRQVLTLTEAAAERIRQLLQQRQRPFLRLGVKARGCNGLSYTLNYADEKGKFDELVEDQDVKILIEPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQCGCGESFMTTGSAEANKK

Homology

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BLAST of MS004153 vs. NCBI nr
Match: XP_022150583.1 (iron-sulfur assembly protein IscA-like 1, mitochondrial [Momordica charantia])

HSP 1 Score: 265.4 bits (677), Expect = 2.7e-67
Identity = 137/137 (100.00%), Postives = 137/137 (100.00%), Query Frame = 0


Query: 1   MAASSSSFFAAASKKVGPAARRQVLTLTEAAAERIRQLLQQRQRPFLRLGVKARGCNGLS 60
           MAASSSSFFAAASKKVGPAARRQVLTLTEAAAERIRQLLQQRQRPFLRLGVKARGCNGLS
Sbjct: 1   MAASSSSFFAAASKKVGPAARRQVLTLTEAAAERIRQLLQQRQRPFLRLGVKARGCNGLS 60

Query: 61  YTLNYADEKGKFDELVEDQDVKILIEPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQC 120
           YTLNYADEKGKFDELVEDQDVKILIEPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQC
Sbjct: 61  YTLNYADEKGKFDELVEDQDVKILIEPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQC 120

Query: 121 GCGESFMTTGSAEANKK 138
           GCGESFMTTGSAEANKK
Sbjct: 121 GCGESFMTTGSAEANKK 137

[Close]

BLAST of MS004153 vs. NCBI nr
Match: XP_022987453.1 (iron-sulfur assembly protein IscA-like 1, mitochondrial [Cucurbita maxima])

HSP 1 Score: 259.2 bits (661), Expect = 2.0e-65
Identity = 133/137 (97.08%), Postives = 136/137 (99.27%), Query Frame = 0


Query: 1   MAASSSSFFAAASKKVGPAARRQVLTLTEAAAERIRQLLQQRQRPFLRLGVKARGCNGLS 60
           MAASSSSFFAAAS+KVGPAARRQ+LTLTEAAA RIRQLLQQRQRPFLRLGVKARGCNGLS
Sbjct: 19  MAASSSSFFAAASQKVGPAARRQILTLTEAAAARIRQLLQQRQRPFLRLGVKARGCNGLS 78

Query: 61  YTLNYADEKGKFDELVEDQDVKILIEPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQC 120
           YTLNYADEKGKFDELVEDQ+VKILIEPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQC
Sbjct: 79  YTLNYADEKGKFDELVEDQNVKILIEPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQC 138

Query: 121 GCGESFMTTGSAEANKK 138
           GCGESFMTTGSAEANKK
Sbjct: 139 GCGESFMTTGSAEANKK 155

[Close]

BLAST of MS004153 vs. NCBI nr
Match: KAG7032017.1 (Iron-sulfur assembly protein IscA-like 1, mitochondrial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 258.8 bits (660), Expect = 2.6e-65
Identity = 132/137 (96.35%), Postives = 136/137 (99.27%), Query Frame = 0


Query: 1   MAASSSSFFAAASKKVGPAARRQVLTLTEAAAERIRQLLQQRQRPFLRLGVKARGCNGLS 60
           MAASSSSFFAAAS+KVGPAARRQ+LTLTEAAA RIRQLLQQRQRPFLRLGVKARGCNGLS
Sbjct: 19  MAASSSSFFAAASQKVGPAARRQILTLTEAAAARIRQLLQQRQRPFLRLGVKARGCNGLS 78

Query: 61  YTLNYADEKGKFDELVEDQDVKILIEPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQC 120
           YTLNYADEKGKFDELVEDQ+VKILIEPKALMHVIGTKMDF+DDKLRSEFIFINPNSKGQC
Sbjct: 79  YTLNYADEKGKFDELVEDQNVKILIEPKALMHVIGTKMDFIDDKLRSEFIFINPNSKGQC 138

Query: 121 GCGESFMTTGSAEANKK 138
           GCGESFMTTGSAEANKK
Sbjct: 139 GCGESFMTTGSAEANKK 155

[Close]

BLAST of MS004153 vs. NCBI nr
Match: XP_038891787.1 (iron-sulfur assembly protein IscA-like 1, mitochondrial isoform X1 [Benincasa hispida])

HSP 1 Score: 258.1 bits (658), Expect = 4.4e-65
Identity = 132/137 (96.35%), Postives = 136/137 (99.27%), Query Frame = 0


Query: 1   MAASSSSFFAAASKKVGPAARRQVLTLTEAAAERIRQLLQQRQRPFLRLGVKARGCNGLS 60
           MAASSSSFFAAAS+KVGPAARRQ+LTLTEAAA RIRQLLQQRQRPFLRLGVKARGCNGLS
Sbjct: 1   MAASSSSFFAAASQKVGPAARRQILTLTEAAAARIRQLLQQRQRPFLRLGVKARGCNGLS 60

Query: 61  YTLNYADEKGKFDELVEDQDVKILIEPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQC 120
           YTLNYADEKGKFDELVEDQ+VKILI+PKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQC
Sbjct: 61  YTLNYADEKGKFDELVEDQNVKILIDPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQC 120

Query: 121 GCGESFMTTGSAEANKK 138
           GCGESFMTTGSAEANKK
Sbjct: 121 GCGESFMTTGSAEANKK 137

[Close]

BLAST of MS004153 vs. NCBI nr
Match: XP_022956980.1 (iron-sulfur assembly protein IscA-like 1, mitochondrial [Cucurbita moschata])

HSP 1 Score: 255.4 bits (651), Expect = 2.8e-64
Identity = 131/137 (95.62%), Postives = 135/137 (98.54%), Query Frame = 0


Query: 1   MAASSSSFFAAASKKVGPAARRQVLTLTEAAAERIRQLLQQRQRPFLRLGVKARGCNGLS 60
           MAASSSSF AAAS+KVGPAARR++LTLTEAAA RIRQLLQQRQRPFLRLGVKARGCNGLS
Sbjct: 1   MAASSSSFLAAASQKVGPAARRKILTLTEAAAARIRQLLQQRQRPFLRLGVKARGCNGLS 60

Query: 61  YTLNYADEKGKFDELVEDQDVKILIEPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQC 120
           YTLNYADEKGKFDELVEDQ+VKILIEPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQC
Sbjct: 61  YTLNYADEKGKFDELVEDQNVKILIEPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQC 120

Query: 121 GCGESFMTTGSAEANKK 138
           GCGESFMTTGSAEANKK
Sbjct: 121 GCGESFMTTGSAEANKK 137

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BLAST of MS004153 vs. ExPASy Swiss-Prot
Match: Q8LBM4 (Iron-sulfur assembly protein IscA-like 1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At2g16710 PE=2 SV=2)

HSP 1 Score: 213.0 bits (541), Expect = 2.1e-54
Identity = 103/132 (78.03%), Postives = 119/132 (90.15%), Query Frame = 0


Query: 5   SSSFFAAASKKVGPAARRQVLTLTEAAAERIRQLLQQRQRPFLRLGVKARGCNGLSYTLN 64
           +S   AAA+ +VGPA R+QVLTLT+ AA R+  LLQQRQ+PFLRLGVKARGCNGLSYTLN
Sbjct: 3   ASQILAAAAARVGPALRKQVLTLTDEAASRVHHLLQQRQKPFLRLGVKARGCNGLSYTLN 62

Query: 65  YADEKGKFDELVEDQDVKILIEPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQCGCGE 124
           YADEKGKFDELVE++ V+IL+EPKALMHVIGTKMDFVDDKLRSEF+FINPNS+GQCGCGE
Sbjct: 63  YADEKGKFDELVEEKGVRILVEPKALMHVIGTKMDFVDDKLRSEFVFINPNSQGQCGCGE 122

Query: 125 SFMTTGSAEANK 137
           SFMTT ++ A +
Sbjct: 123 SFMTTSTSSAKQ 134

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BLAST of MS004153 vs. ExPASy Swiss-Prot
Match: Q8L8C0 (Iron-sulfur assembly protein IscA-like 3, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At2g36260 PE=3 SV=2)

HSP 1 Score: 170.2 bits (430), Expect = 1.6e-41
Identity = 80/109 (73.39%), Postives = 98/109 (89.91%), Query Frame = 0


Query: 21  RRQVLTLTEAAAERIRQLLQQRQRPFLRLGVKARGCNGLSYTLNYADEKGKFDELVEDQD 80
           R+QVL L++ AA RIRQLLQ +Q+PFLRL V+A+GCNGLSY LNYA EKGKFDE+VE++ 
Sbjct: 2   RKQVLALSDTAAARIRQLLQHQQKPFLRLAVEAKGCNGLSYVLNYAQEKGKFDEVVEEKG 61

Query: 81  VKILIEPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQCGCGESFMTT 130
           VKIL++PKA+MHVIGT+MDFVDDKLRSEF+F+NPN+  +CGCGESF TT
Sbjct: 62  VKILVDPKAVMHVIGTEMDFVDDKLRSEFVFVNPNAT-KCGCGESFTTT 109

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BLAST of MS004153 vs. ExPASy Swiss-Prot
Match: Q07821 (Iron-sulfur assembly protein 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ISA1 PE=1 SV=1)

HSP 1 Score: 124.4 bits (311), Expect = 9.9e-28
Identity = 57/107 (53.27%), Postives = 79/107 (73.83%), Query Frame = 0


Query: 21  RRQVLTLTEAAAERIRQLLQQRQRPFLRLGVKARGCNGLSYTLNYADEKGKFDELVEDQD 80
           R+ ++TL+  A + +R LL Q +   +R+  + RGC+GL+Y L Y  E GKFDE+VE   
Sbjct: 143 RKALITLSPKAIKHLRALLAQPEPKLIRVSARNRGCSGLTYDLQYITEPGKFDEVVEQDG 202

Query: 81  VKILIEPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQCGCGESFM 128
           VKI+I+ KAL  +IG++MD++DDKL S+F+F NPNSKG CGCGESFM
Sbjct: 203 VKIVIDSKALFSIIGSEMDWIDDKLASKFVFKNPNSKGTCGCGESFM 249

[Close]

BLAST of MS004153 vs. ExPASy Swiss-Prot
Match: Q54VS1 (Iron-sulfur cluster assembly 1 homolog, mitochondrial OS=Dictyostelium discoideum OX=44689 GN=isca1 PE=3 SV=1)

HSP 1 Score: 122.1 bits (305), Expect = 4.9e-27
Identity = 53/118 (44.92%), Postives = 91/118 (77.12%), Query Frame = 0


Query: 15  KVGPAARRQVLTLTEAAAERIRQLLQQRQRP---FLRLGVKARGCNGLSYTLNYADEKGK 74
           K+G  +++ + ++T++A +R+++++  ++      LRLG+K RGC+G+SYTL++A +K K
Sbjct: 8   KLGKPSKKAIFSMTDSALKRVKEIMNDKKIDNCIGLRLGIKERGCSGMSYTLDFATQKNK 67

Query: 75  FDE-LVEDQDVKILIEPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQCGCGESFMT 129
           FDE +V D+D+ I+++ KAL+ VIGT+MD++++ ++ EFIFINPN+   CGCGESF T
Sbjct: 68  FDETVVADKDINIIVDSKALLSVIGTEMDYIEEPIKKEFIFINPNATNTCGCGESFTT 125

[Close]

BLAST of MS004153 vs. ExPASy Swiss-Prot
Match: Q8T3X9 (Iron-sulfur cluster assembly 1 homolog, mitochondrial OS=Drosophila melanogaster OX=7227 GN=MagR PE=1 SV=1)

HSP 1 Score: 118.6 bits (296), Expect = 5.4e-26
Identity = 64/118 (54.24%), Postives = 81/118 (68.64%), Query Frame = 0


Query: 10  AAASKKVGPAARRQVLTLTEAAAERIRQLLQQR-QRPFLRLGVKARGCNGLSYTLNYADE 69
           A   +K+ P   R  LTLT AA  RI+ LLQ +     L++GV+ RGCNGLSYTL+YA +
Sbjct: 13  AVKGRKLIPT--RAALTLTPAAVLRIKTLLQDKPDMVGLKVGVRQRGCNGLSYTLDYASQ 72

Query: 70  KGKFDELVEDQDVKILIEPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQCGCGESF 127
           K K DE V    VK+ I+ KA + ++GT+MDFV+ KL SEF+F NPN KG CGCGESF
Sbjct: 73  KDKLDEEVVQDGVKVFIDKKAQLSLLGTEMDFVESKLSSEFVFNNPNIKGTCGCGESF 128

[Close]

BLAST of MS004153 vs. ExPASy TrEMBL
Match: A0A6J1D9T8 (iron-sulfur assembly protein IscA-like 1, mitochondrial OS=Momordica charantia OX=3673 GN=LOC111018687 PE=4 SV=1)

HSP 1 Score: 265.4 bits (677), Expect = 1.3e-67
Identity = 137/137 (100.00%), Postives = 137/137 (100.00%), Query Frame = 0


Query: 1   MAASSSSFFAAASKKVGPAARRQVLTLTEAAAERIRQLLQQRQRPFLRLGVKARGCNGLS 60
           MAASSSSFFAAASKKVGPAARRQVLTLTEAAAERIRQLLQQRQRPFLRLGVKARGCNGLS
Sbjct: 1   MAASSSSFFAAASKKVGPAARRQVLTLTEAAAERIRQLLQQRQRPFLRLGVKARGCNGLS 60

Query: 61  YTLNYADEKGKFDELVEDQDVKILIEPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQC 120
           YTLNYADEKGKFDELVEDQDVKILIEPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQC
Sbjct: 61  YTLNYADEKGKFDELVEDQDVKILIEPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQC 120

Query: 121 GCGESFMTTGSAEANKK 138
           GCGESFMTTGSAEANKK
Sbjct: 121 GCGESFMTTGSAEANKK 137

[Close]

BLAST of MS004153 vs. ExPASy TrEMBL
Match: A0A6J1JJH4 (iron-sulfur assembly protein IscA-like 1, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111484969 PE=4 SV=1)

HSP 1 Score: 259.2 bits (661), Expect = 9.5e-66
Identity = 133/137 (97.08%), Postives = 136/137 (99.27%), Query Frame = 0


Query: 1   MAASSSSFFAAASKKVGPAARRQVLTLTEAAAERIRQLLQQRQRPFLRLGVKARGCNGLS 60
           MAASSSSFFAAAS+KVGPAARRQ+LTLTEAAA RIRQLLQQRQRPFLRLGVKARGCNGLS
Sbjct: 19  MAASSSSFFAAASQKVGPAARRQILTLTEAAAARIRQLLQQRQRPFLRLGVKARGCNGLS 78

Query: 61  YTLNYADEKGKFDELVEDQDVKILIEPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQC 120
           YTLNYADEKGKFDELVEDQ+VKILIEPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQC
Sbjct: 79  YTLNYADEKGKFDELVEDQNVKILIEPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQC 138

Query: 121 GCGESFMTTGSAEANKK 138
           GCGESFMTTGSAEANKK
Sbjct: 139 GCGESFMTTGSAEANKK 155

[Close]

BLAST of MS004153 vs. ExPASy TrEMBL
Match: A0A0A0KTF0 (Fe-S_biosyn domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G614650 PE=4 SV=1)

HSP 1 Score: 255.4 bits (651), Expect = 1.4e-64
Identity = 131/137 (95.62%), Postives = 134/137 (97.81%), Query Frame = 0


Query: 1   MAASSSSFFAAASKKVGPAARRQVLTLTEAAAERIRQLLQQRQRPFLRLGVKARGCNGLS 60
           MAASSSSFFAAAS+K GPAARRQ+LTLTEAAA RIRQLLQQRQRPFLRLGVKARGCNGLS
Sbjct: 1   MAASSSSFFAAASQKAGPAARRQILTLTEAAAARIRQLLQQRQRPFLRLGVKARGCNGLS 60

Query: 61  YTLNYADEKGKFDELVEDQDVKILIEPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQC 120
           YTLNYADEKGKFDELVEDQ+VKILIEPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQC
Sbjct: 61  YTLNYADEKGKFDELVEDQNVKILIEPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQC 120

Query: 121 GCGESFMTTGSAEANKK 138
           GCGESFMTT SAEANKK
Sbjct: 121 GCGESFMTTSSAEANKK 137

[Close]

BLAST of MS004153 vs. ExPASy TrEMBL
Match: A0A6J1GZ87 (iron-sulfur assembly protein IscA-like 1, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111458498 PE=4 SV=1)

HSP 1 Score: 255.4 bits (651), Expect = 1.4e-64
Identity = 131/137 (95.62%), Postives = 135/137 (98.54%), Query Frame = 0


Query: 1   MAASSSSFFAAASKKVGPAARRQVLTLTEAAAERIRQLLQQRQRPFLRLGVKARGCNGLS 60
           MAASSSSF AAAS+KVGPAARR++LTLTEAAA RIRQLLQQRQRPFLRLGVKARGCNGLS
Sbjct: 1   MAASSSSFLAAASQKVGPAARRKILTLTEAAAARIRQLLQQRQRPFLRLGVKARGCNGLS 60

Query: 61  YTLNYADEKGKFDELVEDQDVKILIEPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQC 120
           YTLNYADEKGKFDELVEDQ+VKILIEPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQC
Sbjct: 61  YTLNYADEKGKFDELVEDQNVKILIEPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQC 120

Query: 121 GCGESFMTTGSAEANKK 138
           GCGESFMTTGSAEANKK
Sbjct: 121 GCGESFMTTGSAEANKK 137

[Close]

BLAST of MS004153 vs. ExPASy TrEMBL
Match: A0A5A7SVZ7 (Iron-sulfur assembly protein IscA-like 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold475G001970 PE=4 SV=1)

HSP 1 Score: 252.7 bits (644), Expect = 8.9e-64
Identity = 129/137 (94.16%), Postives = 133/137 (97.08%), Query Frame = 0


Query: 1   MAASSSSFFAAASKKVGPAARRQVLTLTEAAAERIRQLLQQRQRPFLRLGVKARGCNGLS 60
           MAASSSSFFAAAS+K GPA RRQ+LTLTEAAA RIRQLLQQRQRPFLRLGVKARGCNGLS
Sbjct: 1   MAASSSSFFAAASQKAGPAVRRQILTLTEAAAARIRQLLQQRQRPFLRLGVKARGCNGLS 60

Query: 61  YTLNYADEKGKFDELVEDQDVKILIEPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQC 120
           YTLNYADEKGKFDELVEDQ+VKILI+PKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQC
Sbjct: 61  YTLNYADEKGKFDELVEDQNVKILIDPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQC 120

Query: 121 GCGESFMTTGSAEANKK 138
           GCGESFMTT SAEANKK
Sbjct: 121 GCGESFMTTSSAEANKK 137

[Close]

BLAST of MS004153 vs. TAIR 10
Match: AT2G16710.1 (Iron-sulphur cluster biosynthesis family protein )

HSP 1 Score: 213.0 bits (541), Expect = 1.5e-55
Identity = 103/132 (78.03%), Postives = 119/132 (90.15%), Query Frame = 0


Query: 5   SSSFFAAASKKVGPAARRQVLTLTEAAAERIRQLLQQRQRPFLRLGVKARGCNGLSYTLN 64
           +S   AAA+ +VGPA R+QVLTLT+ AA R+  LLQQRQ+PFLRLGVKARGCNGLSYTLN
Sbjct: 3   ASQILAAAAARVGPALRKQVLTLTDEAASRVHHLLQQRQKPFLRLGVKARGCNGLSYTLN 62

Query: 65  YADEKGKFDELVEDQDVKILIEPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQCGCGE 124
           YADEKGKFDELVE++ V+IL+EPKALMHVIGTKMDFVDDKLRSEF+FINPNS+GQCGCGE
Sbjct: 63  YADEKGKFDELVEEKGVRILVEPKALMHVIGTKMDFVDDKLRSEFVFINPNSQGQCGCGE 122

Query: 125 SFMTTGSAEANK 137
           SFMTT ++ A +
Sbjct: 123 SFMTTSTSSAKQ 134

[Close]

BLAST of MS004153 vs. TAIR 10
Match: AT2G16710.3 (Iron-sulphur cluster biosynthesis family protein )

HSP 1 Score: 206.5 bits (524), Expect = 1.4e-53
Identity = 103/138 (74.64%), Postives = 119/138 (86.23%), Query Frame = 0


Query: 5   SSSFFAAASKKVGPAARRQVLTLTEAAAERIRQLLQQRQRPFLRLGVKARGCNGLSYTLN 64
           +S   AAA+ +VGPA R+QVLTLT+ AA R+  LLQQRQ+PFLRLGVKARGCNGLSYTLN
Sbjct: 3   ASQILAAAAARVGPALRKQVLTLTDEAASRVHHLLQQRQKPFLRLGVKARGCNGLSYTLN 62

Query: 65  YA------DEKGKFDELVEDQDVKILIEPKALMHVIGTKMDFVDDKLRSEFIFINPNSKG 124
           YA      DEKGKFDELVE++ V+IL+EPKALMHVIGTKMDFVDDKLRSEF+FINPNS+G
Sbjct: 63  YAGKTSGNDEKGKFDELVEEKGVRILVEPKALMHVIGTKMDFVDDKLRSEFVFINPNSQG 122

Query: 125 QCGCGESFMTTGSAEANK 137
           QCGCGESFMTT ++ A +
Sbjct: 123 QCGCGESFMTTSTSSAKQ 140

[Close]

BLAST of MS004153 vs. TAIR 10
Match: AT2G36260.1 (Iron-sulphur cluster biosynthesis family protein )

HSP 1 Score: 170.2 bits (430), Expect = 1.1e-42
Identity = 80/109 (73.39%), Postives = 98/109 (89.91%), Query Frame = 0


Query: 21  RRQVLTLTEAAAERIRQLLQQRQRPFLRLGVKARGCNGLSYTLNYADEKGKFDELVEDQD 80
           R+QVL L++ AA RIRQLLQ +Q+PFLRL V+A+GCNGLSY LNYA EKGKFDE+VE++ 
Sbjct: 2   RKQVLALSDTAAARIRQLLQHQQKPFLRLAVEAKGCNGLSYVLNYAQEKGKFDEVVEEKG 61

Query: 81  VKILIEPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQCGCGESFMTT 130
           VKIL++PKA+MHVIGT+MDFVDDKLRSEF+F+NPN+  +CGCGESF TT
Sbjct: 62  VKILVDPKAVMHVIGTEMDFVDDKLRSEFVFVNPNAT-KCGCGESFTTT 109

[Close]

BLAST of MS004153 vs. TAIR 10
Match: AT2G16710.2 (Iron-sulphur cluster biosynthesis family protein )

HSP 1 Score: 163.3 bits (412), Expect = 1.4e-40
Identity = 81/102 (79.41%), Postives = 92/102 (90.20%), Query Frame = 0


Query: 5   SSSFFAAASKKVGPAARRQVLTLTEAAAERIRQLLQQRQRPFLRLGVKARGCNGLSYTLN 64
           +S   AAA+ +VGPA R+QVLTLT+ AA R+  LLQQRQ+PFLRLGVKARGCNGLSYTLN
Sbjct: 3   ASQILAAAAARVGPALRKQVLTLTDEAASRVHHLLQQRQKPFLRLGVKARGCNGLSYTLN 62

Query: 65  YADEKGKFDELVEDQDVKILIEPKALMHVIGTKMDFVDDKLR 107
           YADEKGKFDELVE++ V+IL+EPKALMHVIGTKMDFVDDKLR
Sbjct: 63  YADEKGKFDELVEEKGVRILVEPKALMHVIGTKMDFVDDKLR 104

[Close]

BLAST of MS004153 vs. TAIR 10
Match: AT1G10500.1 (chloroplast-localized ISCA-like protein )

HSP 1 Score: 70.5 bits (171), Expect = 1.2e-12
Identity = 39/121 (32.23%), Postives = 69/121 (57.02%), Query Frame = 0


Query: 11  AASKKVGPAAR--RQVLTLTEAAAERIRQLLQQRQRPF-LRLGVKARGCNGLSYTLNYAD 70
           +AS    PA    +  ++L+E A + + ++  +R     LR+GVK  GC+G+SYT+++ +
Sbjct: 56  SASVPAAPAMEGLKPAISLSENALKHLSKMRSERGEDLCLRIGVKQGGCSGMSYTMDFEN 115

Query: 71  EKG--KFDELVEDQDVKILIEPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQCGCGES 127
                  D  +E Q   I+ +PK+++ + G ++D+ D  +   F F NPN+   CGCG+S
Sbjct: 116 RANARPDDSTIEYQGFTIVCDPKSMLFLFGMQLDYSDALIGGGFSFSNPNATQTCGCGKS 175

The following BLAST results are available for this feature:

BLAST of MS004153 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022150583.1	2.7e-67	100.00	iron-sulfur assembly protein IscA-like 1, mitochondrial [Momordica charantia]	[more]
XP_022987453.1	2.0e-65	97.08	iron-sulfur assembly protein IscA-like 1, mitochondrial [Cucurbita maxima]	[more]
KAG7032017.1	2.6e-65	96.35	Iron-sulfur assembly protein IscA-like 1, mitochondrial [Cucurbita argyrosperma ...	[more]
XP_038891787.1	4.4e-65	96.35	iron-sulfur assembly protein IscA-like 1, mitochondrial isoform X1 [Benincasa hi...	[more]
XP_022956980.1	2.8e-64	95.62	iron-sulfur assembly protein IscA-like 1, mitochondrial [Cucurbita moschata]	[more]

BLAST of MS004153 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8LBM4	2.1e-54	78.03	Iron-sulfur assembly protein IscA-like 1, mitochondrial OS=Arabidopsis thaliana ...	[more]
Q8L8C0	1.6e-41	73.39	Iron-sulfur assembly protein IscA-like 3, mitochondrial OS=Arabidopsis thaliana ...	[more]
Q07821	9.9e-28	53.27	Iron-sulfur assembly protein 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 /...	[more]
Q54VS1	4.9e-27	44.92	Iron-sulfur cluster assembly 1 homolog, mitochondrial OS=Dictyostelium discoideu...	[more]
Q8T3X9	5.4e-26	54.24	Iron-sulfur cluster assembly 1 homolog, mitochondrial OS=Drosophila melanogaster...	[more]

BLAST of MS004153 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1D9T8	1.3e-67	100.00	iron-sulfur assembly protein IscA-like 1, mitochondrial OS=Momordica charantia O...	[more]
A0A6J1JJH4	9.5e-66	97.08	iron-sulfur assembly protein IscA-like 1, mitochondrial OS=Cucurbita maxima OX=3...	[more]
A0A0A0KTF0	1.4e-64	95.62	Fe-S_biosyn domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G614650...	[more]
A0A6J1GZ87	1.4e-64	95.62	iron-sulfur assembly protein IscA-like 1, mitochondrial OS=Cucurbita moschata OX...	[more]
A0A5A7SVZ7	8.9e-64	94.16	Iron-sulfur assembly protein IscA-like 1 OS=Cucumis melo var. makuwa OX=1194695 ...	[more]

BLAST of MS004153 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G16710.1	1.5e-55	78.03	Iron-sulphur cluster biosynthesis family protein	[more]
AT2G16710.3	1.4e-53	74.64	Iron-sulphur cluster biosynthesis family protein	[more]
AT2G36260.1	1.1e-42	73.39	Iron-sulphur cluster biosynthesis family protein	[more]
AT2G16710.2	1.4e-40	79.41	Iron-sulphur cluster biosynthesis family protein	[more]
AT1G10500.1	1.2e-12	32.23	chloroplast-localized ISCA-like protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000361	FeS cluster biogenesis	PFAM	PF01521	Fe-S_biosyn	coord: 25..124 e-value: 3.3E-21 score: 75.6
IPR035903	HesB-like domain superfamily	GENE3D	2.60.300.12		coord: 24..130 e-value: 6.4E-34 score: 118.4
IPR035903	HesB-like domain superfamily	SUPERFAMILY	89360	HesB-like domain	coord: 24..117
IPR016092	FeS cluster insertion protein	TIGRFAM	TIGR00049	TIGR00049	coord: 25..127 e-value: 1.7E-34 score: 116.2
None	No IPR available	PANTHER	PTHR10072	IRON-SULFUR CLUSTER ASSEMBLY PROTEIN	coord: 1..136
None	No IPR available	PANTHER	PTHR10072:SF60	IRON-SULFUR ASSEMBLY PROTEIN ISCA-LIKE 3, MITOCHONDRIAL	coord: 1..136
IPR017870	FeS cluster insertion, C-terminal, conserved site	PROSITE	PS01152	HESB	coord: 109..126

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS004153.1	MS004153.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016226	iron-sulfur cluster assembly
molecular_function	GO:0051536	iron-sulfur cluster binding