Homology
BLAST of MS004127 vs. NCBI nr
Match:
XP_022150594.1 (very-long-chain enoyl-CoA reductase-like [Momordica charantia])
HSP 1 Score: 520.4 bits (1339), Expect = 9.6e-144
Identity = 264/277 (95.31%), Postives = 264/277 (95.31%), Query Frame = 0
Query: 1 MDILSAFLFPPPPSTFVNVMTVVSSTAMAMSGFSEMNGKHLQYSKFWNANGASKSSSEES 60
MDI SAFLFPPPPSTFVNVMTVVSSTAMAMSGFSEMNGKHLQYSKFWNANGASKSSSEES
Sbjct: 1 MDISSAFLFPPPPSTFVNVMTVVSSTAMAMSGFSEMNGKHLQYSKFWNANGASKSSSEES 60
Query: 61 KVSSKYGMLLAYTPAFLAGAASFWFFPSDDGRILLLKSALTFHFFKRIFEVLSLPLYASV 120
KVSSKYGMLLAYTPAFLAGAASFWFFPSDDGRILLLKSALTFHFFKRIFE
Sbjct: 61 KVSSKYGMLLAYTPAFLAGAASFWFFPSDDGRILLLKSALTFHFFKRIFE---------- 120
Query: 121 LVLFVHKYSSKMVLDTAFKISLSYFLSTAIMIYTQHLSQGFPEPSIDLKNIGIGCFVMGI 180
VLFVHKYSSKMVLDTAFKISLSYFLSTAIMIYTQHLSQGFP PSIDLKNIGIGCFVMGI
Sbjct: 121 -VLFVHKYSSKMVLDTAFKISLSYFLSTAIMIYTQHLSQGFPAPSIDLKNIGIGCFVMGI 180
Query: 181 IGNFYHHYLLSRTRGKGEKEYKIPNGGLFCLVICPHYLFEIIVYFGFAFISQAIFPLCFA 240
IGNFYHHYLLSRTRGKGEKEYKIPNGGLFCLVICPHYLFEIIVYFGFAFISQAIFPLCFA
Sbjct: 181 IGNFYHHYLLSRTRGKGEKEYKIPNGGLFCLVICPHYLFEIIVYFGFAFISQAIFPLCFA 240
Query: 241 TGTALYLAGRSYATRKWYVSKFEHFPNHVKALLPFLF 278
TGTALYLAGRSYATRKWYVSKFEHFPNHVKALLPFLF
Sbjct: 241 TGTALYLAGRSYATRKWYVSKFEHFPNHVKALLPFLF 266
BLAST of MS004127 vs. NCBI nr
Match:
XP_004135052.2 (3-oxo-5-alpha-steroid 4-dehydrogenase 2 [Cucumis sativus] >KAE8648808.1 hypothetical protein Csa_008329 [Cucumis sativus])
HSP 1 Score: 443.4 bits (1139), Expect = 1.5e-120
Identity = 220/277 (79.42%), Postives = 244/277 (88.09%), Query Frame = 0
Query: 1 MDILSAFLFPPPPSTFVNVMTVVSSTAMAMSGFSEMNGKHLQYSKFWNANGASKSSSEES 60
MD+LSAFLFPPPPSTFVNVMTVVS+TA+AM+G SE+NGKHLQYSKFWNANGASK S+EES
Sbjct: 21 MDVLSAFLFPPPPSTFVNVMTVVSTTALAMAGLSEINGKHLQYSKFWNANGASKLSAEES 80
Query: 61 KVSSKYGMLLAYTPAFLAGAASFWFFPSDDGRILLLKSALTFHFFKRIFEVLSLPLYASV 120
KVSSKYGMLLAYTPAFLAGAASFW FPS D R+ LLKSALT HFFKRIFE
Sbjct: 81 KVSSKYGMLLAYTPAFLAGAASFWLFPSGDERVFLLKSALTLHFFKRIFE---------- 140
Query: 121 LVLFVHKYSSKMVLDTAFKISLSYFLSTAIMIYTQHLSQGFPEPSIDLKNIGIGCFVMGI 180
VLF+HKYSSKMVL TA +ISLSYF+STAIMIYTQHLSQG PEP IDLKNIG+G F++GI
Sbjct: 141 -VLFIHKYSSKMVLKTAIQISLSYFMSTAIMIYTQHLSQGLPEPPIDLKNIGVGSFLIGI 200
Query: 181 IGNFYHHYLLSRTRGKGEKEYKIPNGGLFCLVICPHYLFEIIVYFGFAFISQAIFPLCFA 240
IGNFYHHYLLS+TR +G+ YKIP GGLF V+CPHYLFEII YFGFAFISQ ++PLCF+
Sbjct: 201 IGNFYHHYLLSQTRKQGDTAYKIPKGGLFSFVVCPHYLFEIIGYFGFAFISQELYPLCFS 260
Query: 241 TGTALYLAGRSYATRKWYVSKFEHFPNHVKALLPFLF 278
GTA+YLAGRSYATRKWY+SKFE FPN+VKALLPF+F
Sbjct: 261 IGTAVYLAGRSYATRKWYISKFEAFPNNVKALLPFVF 286
BLAST of MS004127 vs. NCBI nr
Match:
XP_022984951.1 (3-oxo-5-alpha-steroid 4-dehydrogenase 1-like [Cucurbita maxima])
HSP 1 Score: 436.4 bits (1121), Expect = 1.8e-118
Identity = 219/277 (79.06%), Postives = 240/277 (86.64%), Query Frame = 0
Query: 1 MDILSAFLFPPPPSTFVNVMTVVSSTAMAMSGFSEMNGKHLQYSKFWNANGASKSSSEES 60
M ILSAFLFPPPPSTFVNVMTVVSSTAM M+G SE+NGKHLQYSKFWNAN + K+ SEE
Sbjct: 1 MGILSAFLFPPPPSTFVNVMTVVSSTAMGMAGISEINGKHLQYSKFWNANSSPKAPSEEP 60
Query: 61 KVSSKYGMLLAYTPAFLAGAASFWFFPSDDGRILLLKSALTFHFFKRIFEVLSLPLYASV 120
K+SSKYGMLLAYTPAFLAGAASFWFFPSD+ RIL+LK+ALT HFFKRIFE
Sbjct: 61 KLSSKYGMLLAYTPAFLAGAASFWFFPSDNERILVLKAALTLHFFKRIFE---------- 120
Query: 121 LVLFVHKYSSKMVLDTAFKISLSYFLSTAIMIYTQHLSQGFPEPSIDLKNIGIGCFVMGI 180
VLF+HKYSSKMV DTA KIS SY LST IMIYTQHLSQG PEP IDLKNIGI F++GI
Sbjct: 121 -VLFIHKYSSKMVFDTAIKISFSYCLSTVIMIYTQHLSQGIPEPPIDLKNIGIALFLIGI 180
Query: 181 IGNFYHHYLLSRTRGKGEKEYKIPNGGLFCLVICPHYLFEIIVYFGFAFISQAIFPLCFA 240
IGNFYHH+LLS+TR GE YKIP GGLF LVICPHYLFEII +FGFAFISQ ++PLCF+
Sbjct: 181 IGNFYHHFLLSKTRKPGETGYKIPKGGLFSLVICPHYLFEIIEFFGFAFISQELYPLCFS 240
Query: 241 TGTALYLAGRSYATRKWYVSKFEHFPNHVKALLPFLF 278
TGTA+YLAGRSYATRKWYVSKFE+FP+HVKALLPF+F
Sbjct: 241 TGTAIYLAGRSYATRKWYVSKFENFPSHVKALLPFVF 266
BLAST of MS004127 vs. NCBI nr
Match:
XP_022957108.1 (3-oxo-5-alpha-steroid 4-dehydrogenase 1-like [Cucurbita moschata])
HSP 1 Score: 436.4 bits (1121), Expect = 1.8e-118
Identity = 219/277 (79.06%), Postives = 240/277 (86.64%), Query Frame = 0
Query: 1 MDILSAFLFPPPPSTFVNVMTVVSSTAMAMSGFSEMNGKHLQYSKFWNANGASKSSSEES 60
M ILSAFLFPPPPSTFVNVMTVVSSTAM M+G SE+NGKHLQYSKFWNAN + K+ SEE
Sbjct: 1 MGILSAFLFPPPPSTFVNVMTVVSSTAMGMAGISEINGKHLQYSKFWNANSSPKAPSEEP 60
Query: 61 KVSSKYGMLLAYTPAFLAGAASFWFFPSDDGRILLLKSALTFHFFKRIFEVLSLPLYASV 120
K+SSKYGMLLAYTPAFLAGAASFWFFPSD+ RIL+LK+ALT HFFKRIFE
Sbjct: 61 KLSSKYGMLLAYTPAFLAGAASFWFFPSDNERILVLKAALTLHFFKRIFE---------- 120
Query: 121 LVLFVHKYSSKMVLDTAFKISLSYFLSTAIMIYTQHLSQGFPEPSIDLKNIGIGCFVMGI 180
VLF+HKYSSKMV DTA KIS SY LST IMIYTQHLSQG PEP IDLKNIGI F++GI
Sbjct: 121 -VLFIHKYSSKMVFDTAIKISFSYCLSTVIMIYTQHLSQGLPEPPIDLKNIGIALFLIGI 180
Query: 181 IGNFYHHYLLSRTRGKGEKEYKIPNGGLFCLVICPHYLFEIIVYFGFAFISQAIFPLCFA 240
IGNFYHH+LLS+TR GE YKIP GGLF LVICPHYLFEII +FGFAFISQ ++PLCF+
Sbjct: 181 IGNFYHHFLLSKTRKPGETGYKIPKGGLFSLVICPHYLFEIIEFFGFAFISQELYPLCFS 240
Query: 241 TGTALYLAGRSYATRKWYVSKFEHFPNHVKALLPFLF 278
TGTA+YLAGRSYATRKWYVSKFE+FP+HVKALLPF+F
Sbjct: 241 TGTAIYLAGRSYATRKWYVSKFENFPSHVKALLPFVF 266
BLAST of MS004127 vs. NCBI nr
Match:
KAG7032032.1 (3-oxo-5-alpha-steroid 4-dehydrogenase 1 [Cucurbita argyrosperma subsp. argyrosperma])
HSP 1 Score: 434.1 bits (1115), Expect = 9.0e-118
Identity = 218/277 (78.70%), Postives = 240/277 (86.64%), Query Frame = 0
Query: 1 MDILSAFLFPPPPSTFVNVMTVVSSTAMAMSGFSEMNGKHLQYSKFWNANGASKSSSEES 60
M ILSAFLFPPP STFVNVMTVVSSTAM M+G SE+NGKHLQYSKFWNAN + K+ SEE+
Sbjct: 300 MGILSAFLFPPPASTFVNVMTVVSSTAMGMAGISEINGKHLQYSKFWNANSSPKAPSEET 359
Query: 61 KVSSKYGMLLAYTPAFLAGAASFWFFPSDDGRILLLKSALTFHFFKRIFEVLSLPLYASV 120
K+SSKYGMLLAYTPAFLAGAASFWFFPSD+ RIL+LK+ALT HFFKRIFE
Sbjct: 360 KLSSKYGMLLAYTPAFLAGAASFWFFPSDNERILVLKAALTLHFFKRIFE---------- 419
Query: 121 LVLFVHKYSSKMVLDTAFKISLSYFLSTAIMIYTQHLSQGFPEPSIDLKNIGIGCFVMGI 180
VLF+HKYSSKMV DTA KIS SY LST IMIYTQHLSQG PEP IDLKNIGI F++GI
Sbjct: 420 -VLFIHKYSSKMVFDTAIKISFSYCLSTVIMIYTQHLSQGLPEPPIDLKNIGIALFLIGI 479
Query: 181 IGNFYHHYLLSRTRGKGEKEYKIPNGGLFCLVICPHYLFEIIVYFGFAFISQAIFPLCFA 240
IGNFYHH+LLS+TR GE YKIP GGLF LVICPHYLFEII +FGFAFISQ ++PLCF+
Sbjct: 480 IGNFYHHFLLSKTRKPGETGYKIPKGGLFSLVICPHYLFEIIEFFGFAFISQELYPLCFS 539
Query: 241 TGTALYLAGRSYATRKWYVSKFEHFPNHVKALLPFLF 278
TGTA+YLAGRSYATRKWYVSKFE+FP+HVKALLPF+F
Sbjct: 540 TGTAIYLAGRSYATRKWYVSKFENFPSHVKALLPFVF 565
BLAST of MS004127 vs. ExPASy Swiss-Prot
Match:
O18765 (3-oxo-5-alpha-steroid 4-dehydrogenase 2 OS=Sus scrofa OX=9823 GN=SRD5A2 PE=2 SV=2)
HSP 1 Score: 77.8 bits (190), Expect = 2.2e-13
Identity = 40/108 (37.04%), Postives = 65/108 (60.19%), Query Frame = 0
Query: 170 NIGIGCFVMGIIGNFYHHYLLSRTRGKGEKEYKIPNGGLFCLVICPHYLFEIIVYFGFAF 229
++G+ F++G+ N + Y+L + R GE YKIP GGLF V ++L EII + G+A
Sbjct: 147 SLGVFLFILGMGINIHSDYILRQLRKPGEVIYKIPQGGLFTYVSGANFLGEIIEWIGYAL 206
Query: 230 ISQAIFPLCFATGTALYLAGRSYATRKWYVSKFEHFPNHVKALLPFLF 278
+ ++ L FA + +L R++ ++YV FE +P KAL+PF+F
Sbjct: 207 ATWSLPALAFAFFSLCFLGLRAFHHHRFYVKMFEDYPKSRKALIPFIF 254
BLAST of MS004127 vs. ExPASy Swiss-Prot
Match:
Q28892 (3-oxo-5-alpha-steroid 4-dehydrogenase 2 OS=Macaca fascicularis OX=9541 GN=SRD5A2 PE=1 SV=1)
HSP 1 Score: 77.0 bits (188), Expect = 3.7e-13
Identity = 44/134 (32.84%), Postives = 75/134 (55.97%), Query Frame = 0
Query: 145 FLSTAIMIYTQHLSQGFPEPSIDLK-NIGIGCFVMGIIGNFYHHYLLSRTRGKGEKEYKI 204
FL + +IY G+ D++ +G+ F++G+ N + Y+L + R GE Y+I
Sbjct: 124 FLQSYYLIYCAEYPDGW---YTDIRFCLGVFLFILGMGVNIHSDYILRQLRKPGEITYRI 183
Query: 205 PNGGLFCLVICPHYLFEIIVYFGFAFISQAIFPLCFATGTALYLAGRSYATRKWYVSKFE 264
P GGLF V ++L EII + G+A + ++ L FA + +L R++ ++Y+ FE
Sbjct: 184 PKGGLFTYVSGANFLGEIIEWIGYALATWSLPALAFAFFSVCFLGLRAFHHHRFYLKMFE 243
Query: 265 HFPNHVKALLPFLF 278
+P KAL+PF+F
Sbjct: 244 DYPKSRKALIPFIF 254
BLAST of MS004127 vs. ExPASy Swiss-Prot
Match:
P31213 (3-oxo-5-alpha-steroid 4-dehydrogenase 2 OS=Homo sapiens OX=9606 GN=SRD5A2 PE=1 SV=1)
HSP 1 Score: 76.3 bits (186), Expect = 6.3e-13
Identity = 38/108 (35.19%), Postives = 65/108 (60.19%), Query Frame = 0
Query: 170 NIGIGCFVMGIIGNFYHHYLLSRTRGKGEKEYKIPNGGLFCLVICPHYLFEIIVYFGFAF 229
++G+ F++G+ N + Y+L + R GE Y+IP GGLF V ++L EII + G+A
Sbjct: 147 SLGVFLFILGMGINIHSDYILRQLRKPGEISYRIPQGGLFTYVSGANFLGEIIEWIGYAL 206
Query: 230 ISQAIFPLCFATGTALYLAGRSYATRKWYVSKFEHFPNHVKALLPFLF 278
+ ++ L FA + +L R++ ++Y+ FE +P KAL+PF+F
Sbjct: 207 ATWSLPALAFAFFSLCFLGLRAFHHHRFYLKMFEDYPKSRKALIPFIF 254
BLAST of MS004127 vs. ExPASy Swiss-Prot
Match:
Q55C17 (Very-long-chain enoyl-CoA reductase OS=Dictyostelium discoideum OX=44689 GN=gpsn2 PE=3 SV=1)
HSP 1 Score: 73.9 bits (180), Expect = 3.1e-12
Identity = 56/181 (30.94%), Postives = 88/181 (48.62%), Query Frame = 0
Query: 101 TFHFFKRIFEVLSLPLYASVLVLFVHKYS-SKMVLDTAFKISLSYFLSTAIMIYTQHLSQ 160
+ H+ KRI+E +FVH++S M + FK Y+ TA++ Y +
Sbjct: 134 SLHYIKRIYE-----------TIFVHRFSHGTMPIFNLFKNCSYYWGCTAMVSYFVN-HP 193
Query: 161 GFPEPSIDLKNIGIGCFVMGIIGNFYHHYLLSRTRGKGEKEYKIPNGGLFCLVICPHYLF 220
+ E I+ +G+G +++G + N+ H L R G E KIP G LF V CP+Y
Sbjct: 194 LYTEAPIERVYLGLGLWIIGEVFNYICHIQLRNLRPAGSTERKIPRGLLFEFVSCPNYTV 253
Query: 221 EIIVYFGFAFISQA----IFPLCFATGTALYLAGRSYATRKWYVSKFEHFPNHVKALLPF 277
EI+ + GF+ ++Q IF L A ++ G+ RK + K +P K L+PF
Sbjct: 254 EILSWIGFSILTQTLTSWIFALMGAAQMWIWAVGKHRRYRKEFGDK---YPKSRKILIPF 299
BLAST of MS004127 vs. ExPASy Swiss-Prot
Match:
Q68FF9 (3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Mus musculus OX=10090 GN=Srd5a1 PE=2 SV=2)
HSP 1 Score: 72.8 bits (177), Expect = 6.9e-12
Identity = 38/105 (36.19%), Postives = 58/105 (55.24%), Query Frame = 0
Query: 172 GIGCFVMGIIGNFYHHYLLSRTRGKGEKEYKIPNGGLFCLVICPHYLFEIIVYFGFAFIS 231
G +++G++ N + ++L R GE YKIP GGLF V +Y E++ + GFA S
Sbjct: 150 GFALWLVGMVINIHSDHILRNLRKPGETGYKIPRGGLFEYVSSANYFGELVEWCGFALAS 209
Query: 232 QAIFPLCFATGTALYLAGRSYATRKWYVSKFEHFPNHVKALLPFL 277
++ + FA T L R+ +WY+ KFE +P K L+PFL
Sbjct: 210 WSLQGVVFALFTLCALFTRARQHHQWYLEKFEDYPKTRKILIPFL 254
BLAST of MS004127 vs. ExPASy TrEMBL
Match:
A0A6J1DAI6 (very-long-chain enoyl-CoA reductase-like OS=Momordica charantia OX=3673 GN=LOC111018692 PE=3 SV=1)
HSP 1 Score: 520.4 bits (1339), Expect = 4.6e-144
Identity = 264/277 (95.31%), Postives = 264/277 (95.31%), Query Frame = 0
Query: 1 MDILSAFLFPPPPSTFVNVMTVVSSTAMAMSGFSEMNGKHLQYSKFWNANGASKSSSEES 60
MDI SAFLFPPPPSTFVNVMTVVSSTAMAMSGFSEMNGKHLQYSKFWNANGASKSSSEES
Sbjct: 1 MDISSAFLFPPPPSTFVNVMTVVSSTAMAMSGFSEMNGKHLQYSKFWNANGASKSSSEES 60
Query: 61 KVSSKYGMLLAYTPAFLAGAASFWFFPSDDGRILLLKSALTFHFFKRIFEVLSLPLYASV 120
KVSSKYGMLLAYTPAFLAGAASFWFFPSDDGRILLLKSALTFHFFKRIFE
Sbjct: 61 KVSSKYGMLLAYTPAFLAGAASFWFFPSDDGRILLLKSALTFHFFKRIFE---------- 120
Query: 121 LVLFVHKYSSKMVLDTAFKISLSYFLSTAIMIYTQHLSQGFPEPSIDLKNIGIGCFVMGI 180
VLFVHKYSSKMVLDTAFKISLSYFLSTAIMIYTQHLSQGFP PSIDLKNIGIGCFVMGI
Sbjct: 121 -VLFVHKYSSKMVLDTAFKISLSYFLSTAIMIYTQHLSQGFPAPSIDLKNIGIGCFVMGI 180
Query: 181 IGNFYHHYLLSRTRGKGEKEYKIPNGGLFCLVICPHYLFEIIVYFGFAFISQAIFPLCFA 240
IGNFYHHYLLSRTRGKGEKEYKIPNGGLFCLVICPHYLFEIIVYFGFAFISQAIFPLCFA
Sbjct: 181 IGNFYHHYLLSRTRGKGEKEYKIPNGGLFCLVICPHYLFEIIVYFGFAFISQAIFPLCFA 240
Query: 241 TGTALYLAGRSYATRKWYVSKFEHFPNHVKALLPFLF 278
TGTALYLAGRSYATRKWYVSKFEHFPNHVKALLPFLF
Sbjct: 241 TGTALYLAGRSYATRKWYVSKFEHFPNHVKALLPFLF 266
BLAST of MS004127 vs. ExPASy TrEMBL
Match:
A0A6J1J6R3 (3-oxo-5-alpha-steroid 4-dehydrogenase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111483043 PE=3 SV=1)
HSP 1 Score: 436.4 bits (1121), Expect = 8.8e-119
Identity = 219/277 (79.06%), Postives = 240/277 (86.64%), Query Frame = 0
Query: 1 MDILSAFLFPPPPSTFVNVMTVVSSTAMAMSGFSEMNGKHLQYSKFWNANGASKSSSEES 60
M ILSAFLFPPPPSTFVNVMTVVSSTAM M+G SE+NGKHLQYSKFWNAN + K+ SEE
Sbjct: 1 MGILSAFLFPPPPSTFVNVMTVVSSTAMGMAGISEINGKHLQYSKFWNANSSPKAPSEEP 60
Query: 61 KVSSKYGMLLAYTPAFLAGAASFWFFPSDDGRILLLKSALTFHFFKRIFEVLSLPLYASV 120
K+SSKYGMLLAYTPAFLAGAASFWFFPSD+ RIL+LK+ALT HFFKRIFE
Sbjct: 61 KLSSKYGMLLAYTPAFLAGAASFWFFPSDNERILVLKAALTLHFFKRIFE---------- 120
Query: 121 LVLFVHKYSSKMVLDTAFKISLSYFLSTAIMIYTQHLSQGFPEPSIDLKNIGIGCFVMGI 180
VLF+HKYSSKMV DTA KIS SY LST IMIYTQHLSQG PEP IDLKNIGI F++GI
Sbjct: 121 -VLFIHKYSSKMVFDTAIKISFSYCLSTVIMIYTQHLSQGIPEPPIDLKNIGIALFLIGI 180
Query: 181 IGNFYHHYLLSRTRGKGEKEYKIPNGGLFCLVICPHYLFEIIVYFGFAFISQAIFPLCFA 240
IGNFYHH+LLS+TR GE YKIP GGLF LVICPHYLFEII +FGFAFISQ ++PLCF+
Sbjct: 181 IGNFYHHFLLSKTRKPGETGYKIPKGGLFSLVICPHYLFEIIEFFGFAFISQELYPLCFS 240
Query: 241 TGTALYLAGRSYATRKWYVSKFEHFPNHVKALLPFLF 278
TGTA+YLAGRSYATRKWYVSKFE+FP+HVKALLPF+F
Sbjct: 241 TGTAIYLAGRSYATRKWYVSKFENFPSHVKALLPFVF 266
BLAST of MS004127 vs. ExPASy TrEMBL
Match:
A0A6J1GZA3 (3-oxo-5-alpha-steroid 4-dehydrogenase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111458588 PE=3 SV=1)
HSP 1 Score: 436.4 bits (1121), Expect = 8.8e-119
Identity = 219/277 (79.06%), Postives = 240/277 (86.64%), Query Frame = 0
Query: 1 MDILSAFLFPPPPSTFVNVMTVVSSTAMAMSGFSEMNGKHLQYSKFWNANGASKSSSEES 60
M ILSAFLFPPPPSTFVNVMTVVSSTAM M+G SE+NGKHLQYSKFWNAN + K+ SEE
Sbjct: 1 MGILSAFLFPPPPSTFVNVMTVVSSTAMGMAGISEINGKHLQYSKFWNANSSPKAPSEEP 60
Query: 61 KVSSKYGMLLAYTPAFLAGAASFWFFPSDDGRILLLKSALTFHFFKRIFEVLSLPLYASV 120
K+SSKYGMLLAYTPAFLAGAASFWFFPSD+ RIL+LK+ALT HFFKRIFE
Sbjct: 61 KLSSKYGMLLAYTPAFLAGAASFWFFPSDNERILVLKAALTLHFFKRIFE---------- 120
Query: 121 LVLFVHKYSSKMVLDTAFKISLSYFLSTAIMIYTQHLSQGFPEPSIDLKNIGIGCFVMGI 180
VLF+HKYSSKMV DTA KIS SY LST IMIYTQHLSQG PEP IDLKNIGI F++GI
Sbjct: 121 -VLFIHKYSSKMVFDTAIKISFSYCLSTVIMIYTQHLSQGLPEPPIDLKNIGIALFLIGI 180
Query: 181 IGNFYHHYLLSRTRGKGEKEYKIPNGGLFCLVICPHYLFEIIVYFGFAFISQAIFPLCFA 240
IGNFYHH+LLS+TR GE YKIP GGLF LVICPHYLFEII +FGFAFISQ ++PLCF+
Sbjct: 181 IGNFYHHFLLSKTRKPGETGYKIPKGGLFSLVICPHYLFEIIEFFGFAFISQELYPLCFS 240
Query: 241 TGTALYLAGRSYATRKWYVSKFEHFPNHVKALLPFLF 278
TGTA+YLAGRSYATRKWYVSKFE+FP+HVKALLPF+F
Sbjct: 241 TGTAIYLAGRSYATRKWYVSKFENFPSHVKALLPFVF 266
BLAST of MS004127 vs. ExPASy TrEMBL
Match:
A0A1S3BFS0 (3-oxo-5-alpha-steroid 4-dehydrogenase 2-like OS=Cucumis melo OX=3656 GN=LOC103489360 PE=3 SV=1)
HSP 1 Score: 433.7 bits (1114), Expect = 5.7e-118
Identity = 217/277 (78.34%), Postives = 237/277 (85.56%), Query Frame = 0
Query: 1 MDILSAFLFPPPPSTFVNVMTVVSSTAMAMSGFSEMNGKHLQYSKFWNANGASKSSSEES 60
MDILSAFLFPPPPSTFVNVMTVVSST++AM+G SE+ GKHLQYSKFWNAN SK S++ES
Sbjct: 11 MDILSAFLFPPPPSTFVNVMTVVSSTSLAMAGLSEIKGKHLQYSKFWNANSGSKLSAQES 70
Query: 61 KVSSKYGMLLAYTPAFLAGAASFWFFPSDDGRILLLKSALTFHFFKRIFEVLSLPLYASV 120
K+SSKYGMLL YTPAFLAGAASFW FPS D R LLKSALT HFFKRIFE
Sbjct: 71 KLSSKYGMLLVYTPAFLAGAASFWLFPSGDERFFLLKSALTLHFFKRIFE---------- 130
Query: 121 LVLFVHKYSSKMVLDTAFKISLSYFLSTAIMIYTQHLSQGFPEPSIDLKNIGIGCFVMGI 180
VLF+HKYSSKMVL TA +IS SYFLSTAIMIYTQHLSQG PEP IDLKNIG+G F+ GI
Sbjct: 131 -VLFIHKYSSKMVLKTAIQISFSYFLSTAIMIYTQHLSQGLPEPPIDLKNIGVGLFLTGI 190
Query: 181 IGNFYHHYLLSRTRGKGEKEYKIPNGGLFCLVICPHYLFEIIVYFGFAFISQAIFPLCFA 240
IGNFYHHYLLS+TR + E YKIP GGLF VICPHYLFEII YFGFAFISQ ++PLCF+
Sbjct: 191 IGNFYHHYLLSQTRKQEETAYKIPKGGLFSFVICPHYLFEIIGYFGFAFISQELYPLCFS 250
Query: 241 TGTALYLAGRSYATRKWYVSKFEHFPNHVKALLPFLF 278
GTA+YLAGRSYATRKWY+SKFE+FPNHVKALLPF+F
Sbjct: 251 IGTAIYLAGRSYATRKWYISKFENFPNHVKALLPFVF 276
BLAST of MS004127 vs. ExPASy TrEMBL
Match:
A0A0A0KTC7 (S5A_REDUCTASE domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G612880 PE=3 SV=1)
HSP 1 Score: 407.9 bits (1047), Expect = 3.4e-110
Identity = 205/277 (74.01%), Postives = 228/277 (82.31%), Query Frame = 0
Query: 1 MDILSAFLFPPPPSTFVNVMTVVSSTAMAMSGFSEMNGKHLQYSKFWNANGASKSSSEES 60
MD+LSAFLFPPPPSTFVNVMTVVS+TA+AM+G SE+NGKHLQYSKFWNANGASK S+EES
Sbjct: 21 MDVLSAFLFPPPPSTFVNVMTVVSTTALAMAGLSEINGKHLQYSKFWNANGASKLSAEES 80
Query: 61 KVSSKYGMLLAYTPAFLAGAASFWFFPSDDGRILLLKSALTFHFFKRIFEVLSLPLYASV 120
KVSSKYGMLLAYTPAFLAGAASFW FPS D R
Sbjct: 81 KVSSKYGMLLAYTPAFLAGAASFWLFPSGDER---------------------------- 140
Query: 121 LVLFVHKYSSKMVLDTAFKISLSYFLSTAIMIYTQHLSQGFPEPSIDLKNIGIGCFVMGI 180
VLF+HKYSSKMVL TA +ISLSYF+STAIMIYTQHLSQG PEP IDLKNIG+G F++GI
Sbjct: 141 -VLFIHKYSSKMVLKTAIQISLSYFMSTAIMIYTQHLSQGLPEPPIDLKNIGVGSFLIGI 200
Query: 181 IGNFYHHYLLSRTRGKGEKEYKIPNGGLFCLVICPHYLFEIIVYFGFAFISQAIFPLCFA 240
IGNFYHHYLLS+TR +G+ YKIP GGLF V+CPHYLFEII YFGFAFISQ ++PLCF+
Sbjct: 201 IGNFYHHYLLSQTRKQGDTAYKIPKGGLFSFVVCPHYLFEIIGYFGFAFISQELYPLCFS 260
Query: 241 TGTALYLAGRSYATRKWYVSKFEHFPNHVKALLPFLF 278
GTA+YLAGRSYATRKWY+SKFE FPN+VKALLPF+F
Sbjct: 261 IGTAVYLAGRSYATRKWYISKFEAFPNNVKALLPFVF 268
BLAST of MS004127 vs. TAIR 10
Match:
AT5G16010.1 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )
HSP 1 Score: 303.9 bits (777), Expect = 1.3e-82
Identity = 158/283 (55.83%), Postives = 204/283 (72.08%), Query Frame = 0
Query: 1 MDILSAFLFPPPPSTFVNVMTVVSSTAMAMSGFSEMNGKHLQYSKFWNANGASKSSSEES 60
M+++++F++PPPPS +N M+VV A+A G+SE+ G HL+YSKF G S SS +
Sbjct: 1 MEMVTSFVYPPPPSILLNCMSVVGVAALANIGWSEIRGNHLKYSKF----GVSSSSPQPQ 60
Query: 61 K-----VSSKYGMLLAYTPAFLAGAASFWFFPSDDGRILLLKSALTFHFFKRIFEVLSLP 120
K +SS+ GMLL YTPAFLA A+SF+ PSDD R LLLKSAL HFFKR+FE
Sbjct: 61 KERFGSISSRNGMLLLYTPAFLAAASSFFVVPSDDLRFLLLKSALALHFFKRVFE----- 120
Query: 121 LYASVLVLFVHKYSSKMVLDTAFKISLSYFLSTAIMIYTQHLSQGFPEPSIDLKNIGIGC 180
VLF+HKYS M +D+A IS SYF STA+M+Y+Q+L+ G EPS D+K G+
Sbjct: 121 ------VLFIHKYSGGMAIDSALTISSSYFSSTALMLYSQNLTLGLTEPSFDMKLAGVVM 180
Query: 181 FVMGIIGNFYHHYLLSRTRGK-GEKEYKIPNGGLFCLVICPHYLFEIIVYFGFAFISQAI 240
FV+GI+GN YHH LL++ R + G+KEYKIP GGLF ++ICPHYLFEI+V++ F ISQ I
Sbjct: 181 FVVGIVGNLYHHVLLAKLRKEDGKKEYKIPKGGLFDIIICPHYLFEILVFWSFFLISQTI 240
Query: 241 FPLCFATGTALYLAGRSYATRKWYVSKFEHFPNHVKALLPFLF 278
+ FA GT LYL GRSYATR WY+SKF+ FP H+KAL+PF+F
Sbjct: 241 YSFSFAMGTMLYLIGRSYATRTWYLSKFDDFPKHIKALIPFVF 268
BLAST of MS004127 vs. TAIR 10
Match:
AT2G38050.1 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )
HSP 1 Score: 64.7 bits (156), Expect = 1.3e-10
Identity = 38/108 (35.19%), Postives = 59/108 (54.63%), Query Frame = 0
Query: 171 IGIGCFVMGIIGNFYHHYLLSRTRGKGEKEYKIPNGGLFCLVICPHYLFEIIVYFGFAFI 230
IG+ F+ G+ N L R + + Y IP GG F LV CP+Y E I + G+A +
Sbjct: 155 IGMVVFITGMYINITSDRTLVRLKKENRGGYVIPRGGWFELVSCPNYFGEAIEWLGWAVM 214
Query: 231 SQAIFPLCFATGTALYLAGRSYATRKWYVSKF-EHFPNHVKALLPFLF 278
+ + + F T L R+ A+ KWY++KF E +P KA++PF++
Sbjct: 215 TWSWAGIGFFLYTCSNLFPRARASHKWYIAKFKEEYPKTRKAVIPFVY 262
BLAST of MS004127 vs. TAIR 10
Match:
AT1G72590.1 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )
HSP 1 Score: 57.0 bits (136), Expect = 2.8e-08
Identity = 41/117 (35.04%), Postives = 57/117 (48.72%), Query Frame = 0
Query: 168 LKNIGIGCFVMGIIGNFYHHYLLSRTR--GKGEKEYKIPNGGLFCLVICPHYLFEIIVYF 227
L+ IG F+ G I H +L R KEY IP G F +V CPH+L EI++Y
Sbjct: 207 LQWIGGAIFLWGWIHQRRCHAILGSLREYPSQAKEYIIPYGDWFEMVSCPHFLAEIVLYL 266
Query: 228 GFAFISQ----AIFPLC-FATGTALYLAGRSYATRKWYVSKFEHFPNHVKALLPFLF 278
G S +I+ L F Y AG T +WY+ KFE++P A+ P ++
Sbjct: 267 GLLISSGGTDISIWLLFGFVAANLTYAAGE---THRWYLQKFENYPASRHAIFPHVY 320
BLAST of MS004127 vs. TAIR 10
Match:
AT2G16530.1 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )
HSP 1 Score: 55.1 bits (131), Expect = 1.1e-07
Identity = 38/117 (32.48%), Postives = 54/117 (46.15%), Query Frame = 0
Query: 168 LKNIGIGCFVMGIIGNFYHHYLLS--RTRGKGEKEYKIPNGGLFCLVICPHYLFEIIVYF 227
L+ IG F+ G I H +L R KEY IP G F +V PH+L EI++Y
Sbjct: 230 LQWIGGAIFLWGWIHQRRCHAILGSLRENPSQAKEYIIPYGDWFGMVSSPHFLAEIVLYA 289
Query: 228 GFAFISQAI-----FPLCFATGTALYLAGRSYATRKWYVSKFEHFPNHVKALLPFLF 278
G S F Y AG T +WY+ KFE++P + A+ P+++
Sbjct: 290 GLLIASGGTDITIWLLFGFVAANLTYAAGE---THRWYLRKFENYPANRHAIFPYVY 343
BLAST of MS004127 vs. TAIR 10
Match:
AT2G16530.2 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )
HSP 1 Score: 55.1 bits (131), Expect = 1.1e-07
Identity = 38/117 (32.48%), Postives = 54/117 (46.15%), Query Frame = 0
Query: 168 LKNIGIGCFVMGIIGNFYHHYLLS--RTRGKGEKEYKIPNGGLFCLVICPHYLFEIIVYF 227
L+ IG F+ G I H +L R KEY IP G F +V PH+L EI++Y
Sbjct: 229 LQWIGGAIFLWGWIHQRRCHAILGSLRENPSQAKEYIIPYGDWFGMVSSPHFLAEIVLYA 288
Query: 228 GFAFISQAI-----FPLCFATGTALYLAGRSYATRKWYVSKFEHFPNHVKALLPFLF 278
G S F Y AG T +WY+ KFE++P + A+ P+++
Sbjct: 289 GLLIASGGTDITIWLLFGFVAANLTYAAGE---THRWYLRKFENYPANRHAIFPYVY 342
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
XP_022150594.1 | 9.6e-144 | 95.31 | very-long-chain enoyl-CoA reductase-like [Momordica charantia] | [more] |
XP_004135052.2 | 1.5e-120 | 79.42 | 3-oxo-5-alpha-steroid 4-dehydrogenase 2 [Cucumis sativus] >KAE8648808.1 hypothet... | [more] |
XP_022984951.1 | 1.8e-118 | 79.06 | 3-oxo-5-alpha-steroid 4-dehydrogenase 1-like [Cucurbita maxima] | [more] |
XP_022957108.1 | 1.8e-118 | 79.06 | 3-oxo-5-alpha-steroid 4-dehydrogenase 1-like [Cucurbita moschata] | [more] |
KAG7032032.1 | 9.0e-118 | 78.70 | 3-oxo-5-alpha-steroid 4-dehydrogenase 1 [Cucurbita argyrosperma subsp. argyrospe... | [more] |
Match Name | E-value | Identity | Description | |
O18765 | 2.2e-13 | 37.04 | 3-oxo-5-alpha-steroid 4-dehydrogenase 2 OS=Sus scrofa OX=9823 GN=SRD5A2 PE=2 SV=... | [more] |
Q28892 | 3.7e-13 | 32.84 | 3-oxo-5-alpha-steroid 4-dehydrogenase 2 OS=Macaca fascicularis OX=9541 GN=SRD5A2... | [more] |
P31213 | 6.3e-13 | 35.19 | 3-oxo-5-alpha-steroid 4-dehydrogenase 2 OS=Homo sapiens OX=9606 GN=SRD5A2 PE=1 S... | [more] |
Q55C17 | 3.1e-12 | 30.94 | Very-long-chain enoyl-CoA reductase OS=Dictyostelium discoideum OX=44689 GN=gpsn... | [more] |
Q68FF9 | 6.9e-12 | 36.19 | 3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Mus musculus OX=10090 GN=Srd5a1 PE=2 ... | [more] |
Match Name | E-value | Identity | Description | |
A0A6J1DAI6 | 4.6e-144 | 95.31 | very-long-chain enoyl-CoA reductase-like OS=Momordica charantia OX=3673 GN=LOC11... | [more] |
A0A6J1J6R3 | 8.8e-119 | 79.06 | 3-oxo-5-alpha-steroid 4-dehydrogenase 1-like OS=Cucurbita maxima OX=3661 GN=LOC1... | [more] |
A0A6J1GZA3 | 8.8e-119 | 79.06 | 3-oxo-5-alpha-steroid 4-dehydrogenase 1-like OS=Cucurbita moschata OX=3662 GN=LO... | [more] |
A0A1S3BFS0 | 5.7e-118 | 78.34 | 3-oxo-5-alpha-steroid 4-dehydrogenase 2-like OS=Cucumis melo OX=3656 GN=LOC10348... | [more] |
A0A0A0KTC7 | 3.4e-110 | 74.01 | S5A_REDUCTASE domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G6128... | [more] |
Match Name | E-value | Identity | Description | |
AT5G16010.1 | 1.3e-82 | 55.83 | 3-oxo-5-alpha-steroid 4-dehydrogenase family protein | [more] |
AT2G38050.1 | 1.3e-10 | 35.19 | 3-oxo-5-alpha-steroid 4-dehydrogenase family protein | [more] |
AT1G72590.1 | 2.8e-08 | 35.04 | 3-oxo-5-alpha-steroid 4-dehydrogenase family protein | [more] |
AT2G16530.1 | 1.1e-07 | 32.48 | 3-oxo-5-alpha-steroid 4-dehydrogenase family protein | [more] |
AT2G16530.2 | 1.1e-07 | 32.48 | 3-oxo-5-alpha-steroid 4-dehydrogenase family protein | [more] |