MS003890 (gene) Bitter gourd (TR) v1

Overview
NameMS003890
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionIAA-amino acid hydrolase ILR1-like 4
Locationscaffold127: 1298052 .. 1301194 (-)
RNA-Seq ExpressionMS003890
SyntenyMS003890
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ACTGCGAAATCAATGCGTCCACGCAGATGGTCGAATTTGATTTTCATTTTCAATGCGCTTCTGCCATTGCTAATCCCTTCGAGTTCTTCTCCGAGTCCTGAAGAGCTGTCTCAAATCCCTTTGAATTTCCTTCAATATGCTAAAAATCCCGAGGTTTTTAATTGGATGGTTGATATCAGACGGCAAATACACGAGAACCCTGAACTGGGTTTCGAGGAATTCGAGACCAGTAAGCTTATCAGAGCGGAGCTCGATCGTTTAGACATTTCTTATAAATATCCAGTTGCAATTACCGGCGTAGTTGGTTTCATAGGGACTGGAAACCCACCTTTTGTTGCAATTCGGGCAGACATGGACGCTCTGGCCCTGCAGGTATGCTTCATAGCCAGAAAATTTTAATCCCATCCTTCCTGTTTATTTTGGTTTAATGTAATTATGAAATTGATTATGAACTCGCCTAGTAGCCATTGGGATTTCAATGTTGTAGAGTAGACCGGACACGCACTGATGATCACATGACCTCGATCAAGAAGATGAGTTTTGGGCCGAAATTATATAATTATATGGAGGATTTTGAAACTTGTAGGATTTCTTCAACCGATGTACTTGCCACTGGATTTCACCCATTGTTGTACATTGATCATATCTAACTGGCATGCTATGAAACTTTGGGAGGACAATTCTCAAAATATAATGGGGGGTTTTGGTGGTTATAGGAGGGCGTAGAATGGGAACACAAAAGCAAAGTTCCTGGAAAGATGCACGCATGCGGGCATGATGCTCACGTTGCCATGCTTCTTGGGGCTGCAAAGATACTTCAAGAACACCGCCGCCTAATACAGGTACTTCCACTTGGAACTTGGGTGGTTGGCATCACAATTTGCCTTTTTACCGTGTGATTTTGCTGTCGATGGAAAATTTACATAGTGGTTTATCATCTTCCAAGTTAAATGGGTTGAATAGTGCCATGGAGATTACATTTTAGATTGCTTTCTCATTCTAACATAGGAGTTTGTAGAGTTTTCATATACGTATTATTCTTCTTGTTTCGAAATTCCTAAACTCGATCCATTCAAATATAGAGCTTTATAGGCTTTTTTTCTCCTCTGGGGACTCCCAAATTTGCATTAATTCCCTCAAGTCAGAGAATAAGGCAAAGACAAATCTGACCCCACCTCATTCAGTTGCTTTGCTCAATTGAATCGGTGTTAGTGTTTATTAAACACTGTTTATTAAGCACAAAGACTAATTCATTGCCAACATAGACACTTTTTTCTTTATATAAGGTGAACAAATAGACGAACTTACTTGAACAAAATCTGTTCTATAGGTTGTACTGCTTTGTCATTGAGTTCTATAAGGTGAACAAATAGAATATAGAGGCCTTTTCTTTACCAAAACAAGATTTATATTGATGCTTGTGACAGGCCAGATATTGTTCATACTGCTATGCTAAATTTATGCTGTTGAGAGTCCTGATTTTAACAGGGAACAGTAGTTCTTGTTTTTCAACCAGCTGAGGAAGGAGGTGGGGGAGCAAAGAAAATGTTAGAGTCTGGAGTACTGGATAATGTTGAGGCCATCTTTGGATTGCATTTAACTGGCAAGTGTCCTATTGGTAGGGTGGCTTCCAAGCCCGGCCCTATTCTGGCTGCAAGTGGTTTCTTTGAGGCAGTTATTAGCGGTAAGGGAGGTCATGCTGCTCTACCCCAGCACACAATTGACCCAATTTTAGCAGCTTCCAACATAATTATCAGTTTGCAACATCTCATCTCGCGCGAAGCTGATCCTTTGGATTCACAGGTTCTATATCTCTCTAAAGATTTCTCCTCAGGCTTATAGTCACTGCAACTGTTGAGAAAACATCATTTACATTGGGGGGGAAATTATAGTTGATTTCTTTAAACTCTTCCAATGCCAATTTTAGGTGTGGAGTCCAATGGAAAATCTCAAGTACTGTCTTTTTCAAAGCTTAATTCCTTAATTTGTCGCAAGGTTTAGTATTTGCATCAGTTCGAAATTCTGGGGATTCAACTTTCATCACATTTAATGAGATTTAAGTTTGTTTTTTGTGAAATTTTGGGGTTTCATATAAATATCTAATTCTTCTTCCAGTAGCTTTGCGTGATGGGAGTTTTCAAGACTCAGGACACACTGAGATTTCAGAAAATGGATTAAGAGTTTATGATTTTGAAAGTGGTAAAAACTACTTTTAACCAATTAATATCACTTTTTAAAATTTTTATAATGTTTGGTTAAATAATCAAAAGTGATTCTAGAAATTCTGAAAATACTTAAAAATCAATCAAGGTGCTTATAAAAGAAGCGTTTTACTGAAAAAAAAATTCAAAAATCATCCCATTTTTGCTTTTTTTTCCTGATTTCTAGTTTGACAAAGAACTACTGTGATTGTTCACTTTTCTATTTTGGTTAAAATTAAACACTCTTGTCTATATAATTATGAGAAGGGGGCAGCACCTTAGTTATGTCAATGGTAGCATTTCACAAACATGGAAGCAAAATCTTGCAGGTCGTCACAGTGGCAAAATTTCAGGGCGGTGGGGCATTCAATGTCATTCCAGATTCTGTTACAATTGGCGGTACTTTCAGGGCATTTTCAGACGAAAGCTTCATCCAACTTAAACAGAGAATTGTAGAGGTAGATTTCTAGAAATATGCTGCTTCTTCAAGTCAAAATTCATTTCCAAAAATAGTTCCATAAATACTTTCTTCTTGGAAAAACATGCCAGGTTATCACACGACAAGCCAACGTTCAGAGGTGCAATGCCACTGTCCAGTTCAATGAAGAGAAGCCTTTCTTTCCAGTTACACTAAATGACCCAGATCTTTACAAGCATTTTCATTCAGTCGCTGGAGACATGCTCGGTACCAAGAACATTCTTGATATGCAACCTATAATGGGAACAGAAGACTTTGCATTTTATGGAGAAGTTATACCAGGATTCTTCTTCTTTGTTGGGATGAAGAATGAAACTCTTGGGATGTTCGAATCAGGACATTCACCTTATTATATCGTAAACGAAGACGTGCTTCCTTACGGTGCAGCATTGCATGCTTCAATTGCCACAAGGTACCTTCTGCCCAAATCTACTTTAATTAAAGGAAACTCTCATGATGAGCTT

mRNA sequence

ACTGCGAAATCAATGCGTCCACGCAGATGGTCGAATTTGATTTTCATTTTCAATGCGCTTCTGCCATTGCTAATCCCTTCGAGTTCTTCTCCGAGTCCTGAAGAGCTGTCTCAAATCCCTTTGAATTTCCTTCAATATGCTAAAAATCCCGAGGTTTTTAATTGGATGGTTGATATCAGACGGCAAATACACGAGAACCCTGAACTGGGTTTCGAGGAATTCGAGACCAGTAAGCTTATCAGAGCGGAGCTCGATCGTTTAGACATTTCTTATAAATATCCAGTTGCAATTACCGGCGTAGTTGGTTTCATAGGGACTGGAAACCCACCTTTTGTTGCAATTCGGGCAGACATGGACGCTCTGGCCCTGCAGGAGGGCGTAGAATGGGAACACAAAAGCAAAGTTCCTGGAAAGATGCACGCATGCGGGCATGATGCTCACGTTGCCATGCTTCTTGGGGCTGCAAAGATACTTCAAGAACACCGCCGCCTAATACAGGGAACAGTAGTTCTTGTTTTTCAACCAGCTGAGGAAGGAGGTGGGGGAGCAAAGAAAATGTTAGAGTCTGGAGTACTGGATAATGTTGAGGCCATCTTTGGATTGCATTTAACTGGCAAGTGTCCTATTGGTAGGGTGGCTTCCAAGCCCGGCCCTATTCTGGCTGCAAGTGGTTTCTTTGAGGCAGTTATTAGCGGTAAGGGAGGTCATGCTGCTCTACCCCAGCACACAATTGACCCAATTTTAGCAGCTTCCAACATAATTATCAGTTTGCAACATCTCATCTCGCGCGAAGCTGATCCTTTGGATTCACAGGTCGTCACAGTGGCAAAATTTCAGGGCGGTGGGGCATTCAATGTCATTCCAGATTCTGTTACAATTGGCGGTACTTTCAGGGCATTTTCAGACGAAAGCTTCATCCAACTTAAACAGAGAATTGTAGAGGTTATCACACGACAAGCCAACGTTCAGAGGTGCAATGCCACTGTCCAGTTCAATGAAGAGAAGCCTTTCTTTCCAGTTACACTAAATGACCCAGATCTTTACAAGCATTTTCATTCAGTCGCTGGAGACATGCTCGGTACCAAGAACATTCTTGATATGCAACCTATAATGGGAACAGAAGACTTTGCATTTTATGGAGAAGTTATACCAGGATTCTTCTTCTTTGTTGGGATGAAGAATGAAACTCTTGGGATGTTCGAATCAGGACATTCACCTTATTATATCGTAAACGAAGACGTGCTTCCTTACGGTGCAGCATTGCATGCTTCAATTGCCACAAGGTACCTTCTGCCCAAATCTACTTTAATTAAAGGAAACTCTCATGATGAGCTT

Coding sequence (CDS)

ACTGCGAAATCAATGCGTCCACGCAGATGGTCGAATTTGATTTTCATTTTCAATGCGCTTCTGCCATTGCTAATCCCTTCGAGTTCTTCTCCGAGTCCTGAAGAGCTGTCTCAAATCCCTTTGAATTTCCTTCAATATGCTAAAAATCCCGAGGTTTTTAATTGGATGGTTGATATCAGACGGCAAATACACGAGAACCCTGAACTGGGTTTCGAGGAATTCGAGACCAGTAAGCTTATCAGAGCGGAGCTCGATCGTTTAGACATTTCTTATAAATATCCAGTTGCAATTACCGGCGTAGTTGGTTTCATAGGGACTGGAAACCCACCTTTTGTTGCAATTCGGGCAGACATGGACGCTCTGGCCCTGCAGGAGGGCGTAGAATGGGAACACAAAAGCAAAGTTCCTGGAAAGATGCACGCATGCGGGCATGATGCTCACGTTGCCATGCTTCTTGGGGCTGCAAAGATACTTCAAGAACACCGCCGCCTAATACAGGGAACAGTAGTTCTTGTTTTTCAACCAGCTGAGGAAGGAGGTGGGGGAGCAAAGAAAATGTTAGAGTCTGGAGTACTGGATAATGTTGAGGCCATCTTTGGATTGCATTTAACTGGCAAGTGTCCTATTGGTAGGGTGGCTTCCAAGCCCGGCCCTATTCTGGCTGCAAGTGGTTTCTTTGAGGCAGTTATTAGCGGTAAGGGAGGTCATGCTGCTCTACCCCAGCACACAATTGACCCAATTTTAGCAGCTTCCAACATAATTATCAGTTTGCAACATCTCATCTCGCGCGAAGCTGATCCTTTGGATTCACAGGTCGTCACAGTGGCAAAATTTCAGGGCGGTGGGGCATTCAATGTCATTCCAGATTCTGTTACAATTGGCGGTACTTTCAGGGCATTTTCAGACGAAAGCTTCATCCAACTTAAACAGAGAATTGTAGAGGTTATCACACGACAAGCCAACGTTCAGAGGTGCAATGCCACTGTCCAGTTCAATGAAGAGAAGCCTTTCTTTCCAGTTACACTAAATGACCCAGATCTTTACAAGCATTTTCATTCAGTCGCTGGAGACATGCTCGGTACCAAGAACATTCTTGATATGCAACCTATAATGGGAACAGAAGACTTTGCATTTTATGGAGAAGTTATACCAGGATTCTTCTTCTTTGTTGGGATGAAGAATGAAACTCTTGGGATGTTCGAATCAGGACATTCACCTTATTATATCGTAAACGAAGACGTGCTTCCTTACGGTGCAGCATTGCATGCTTCAATTGCCACAAGGTACCTTCTGCCCAAATCTACTTTAATTAAAGGAAACTCTCATGATGAGCTT

Protein sequence

TAKSMRPRRWSNLIFIFNALLPLLIPSSSSPSPEELSQIPLNFLQYAKNPEVFNWMVDIRRQIHENPELGFEEFETSKLIRAELDRLDISYKYPVAITGVVGFIGTGNPPFVAIRADMDALALQEGVEWEHKSKVPGKMHACGHDAHVAMLLGAAKILQEHRRLIQGTVVLVFQPAEEGGGGAKKMLESGVLDNVEAIFGLHLTGKCPIGRVASKPGPILAASGFFEAVISGKGGHAALPQHTIDPILAASNIIISLQHLISREADPLDSQVVTVAKFQGGGAFNVIPDSVTIGGTFRAFSDESFIQLKQRIVEVITRQANVQRCNATVQFNEEKPFFPVTLNDPDLYKHFHSVAGDMLGTKNILDMQPIMGTEDFAFYGEVIPGFFFFVGMKNETLGMFESGHSPYYIVNEDVLPYGAALHASIATRYLLPKSTLIKGNSHDEL
Homology
BLAST of MS003890 vs. NCBI nr
Match: XP_022145035.1 (IAA-amino acid hydrolase ILR1-like 4 [Momordica charantia])

HSP 1 Score: 891.7 bits (2303), Expect = 2.5e-255
Identity = 441/441 (100.00%), Postives = 441/441 (100.00%), Query Frame = 0

Query: 5   MRPRRWSNLIFIFNALLPLLIPSSSSPSPEELSQIPLNFLQYAKNPEVFNWMVDIRRQIH 64
           MRPRRWSNLIFIFNALLPLLIPSSSSPSPEELSQIPLNFLQYAKNPEVFNWMVDIRRQIH
Sbjct: 1   MRPRRWSNLIFIFNALLPLLIPSSSSPSPEELSQIPLNFLQYAKNPEVFNWMVDIRRQIH 60

Query: 65  ENPELGFEEFETSKLIRAELDRLDISYKYPVAITGVVGFIGTGNPPFVAIRADMDALALQ 124
           ENPELGFEEFETSKLIRAELDRLDISYKYPVAITGVVGFIGTGNPPFVAIRADMDALALQ
Sbjct: 61  ENPELGFEEFETSKLIRAELDRLDISYKYPVAITGVVGFIGTGNPPFVAIRADMDALALQ 120

Query: 125 EGVEWEHKSKVPGKMHACGHDAHVAMLLGAAKILQEHRRLIQGTVVLVFQPAEEGGGGAK 184
           EGVEWEHKSKVPGKMHACGHDAHVAMLLGAAKILQEHRRLIQGTVVLVFQPAEEGGGGAK
Sbjct: 121 EGVEWEHKSKVPGKMHACGHDAHVAMLLGAAKILQEHRRLIQGTVVLVFQPAEEGGGGAK 180

Query: 185 KMLESGVLDNVEAIFGLHLTGKCPIGRVASKPGPILAASGFFEAVISGKGGHAALPQHTI 244
           KMLESGVLDNVEAIFGLHLTGKCPIGRVASKPGPILAASGFFEAVISGKGGHAALPQHTI
Sbjct: 181 KMLESGVLDNVEAIFGLHLTGKCPIGRVASKPGPILAASGFFEAVISGKGGHAALPQHTI 240

Query: 245 DPILAASNIIISLQHLISREADPLDSQVVTVAKFQGGGAFNVIPDSVTIGGTFRAFSDES 304
           DPILAASNIIISLQHLISREADPLDSQVVTVAKFQGGGAFNVIPDSVTIGGTFRAFSDES
Sbjct: 241 DPILAASNIIISLQHLISREADPLDSQVVTVAKFQGGGAFNVIPDSVTIGGTFRAFSDES 300

Query: 305 FIQLKQRIVEVITRQANVQRCNATVQFNEEKPFFPVTLNDPDLYKHFHSVAGDMLGTKNI 364
           FIQLKQRIVEVITRQANVQRCNATVQFNEEKPFFPVTLNDPDLYKHFHSVAGDMLGTKNI
Sbjct: 301 FIQLKQRIVEVITRQANVQRCNATVQFNEEKPFFPVTLNDPDLYKHFHSVAGDMLGTKNI 360

Query: 365 LDMQPIMGTEDFAFYGEVIPGFFFFVGMKNETLGMFESGHSPYYIVNEDVLPYGAALHAS 424
           LDMQPIMGTEDFAFYGEVIPGFFFFVGMKNETLGMFESGHSPYYIVNEDVLPYGAALHAS
Sbjct: 361 LDMQPIMGTEDFAFYGEVIPGFFFFVGMKNETLGMFESGHSPYYIVNEDVLPYGAALHAS 420

Query: 425 IATRYLLPKSTLIKGNSHDEL 446
           IATRYLLPKSTLIKGNSHDEL
Sbjct: 421 IATRYLLPKSTLIKGNSHDEL 441

BLAST of MS003890 vs. NCBI nr
Match: XP_038878754.1 (IAA-amino acid hydrolase ILR1-like 4 [Benincasa hispida])

HSP 1 Score: 775.8 bits (2002), Expect = 2.0e-220
Identity = 379/440 (86.14%), Postives = 411/440 (93.41%), Query Frame = 0

Query: 10  WSNLIFIFNALLPLLIPSSSSPSPEELSQIPLNFLQYAKNPEVFNWMVDIRRQIHENPEL 69
           W NLI IF+ L+PLLIPSSSSP+P+ELSQIP  FLQYAK+PEVF+WMVD+RR+IHENPEL
Sbjct: 6   WLNLILIFHLLIPLLIPSSSSPNPDELSQIPSIFLQYAKHPEVFDWMVDVRRKIHENPEL 65

Query: 70  GFEEFETSKLIRAELDRLDISYKYPVAITGVVGFIGTGNPPFVAIRADMDALALQEGVEW 129
           GFEEFETSKLIRAELDRL ISY+YPVAITGVVG IG GNPPFVAIRADMDALALQEGVEW
Sbjct: 66  GFEEFETSKLIRAELDRLGISYEYPVAITGVVGLIGNGNPPFVAIRADMDALALQEGVEW 125

Query: 130 EHKSKVPGKMHACGHDAHVAMLLGAAKILQEHRRLIQGTVVLVFQPAEEGGGGAKKMLES 189
           EHKSKVPGKMHACGHDAHVAMLLGAAKILQ+HR LIQGTVVLVFQPAEEGGGGAKKMLE+
Sbjct: 126 EHKSKVPGKMHACGHDAHVAMLLGAAKILQQHRHLIQGTVVLVFQPAEEGGGGAKKMLEA 185

Query: 190 GVLDNVEAIFGLHLTGKCPIGRVASKPGPILAASGFFEAVISGKGGHAALPQHTIDPILA 249
           GVLDNV+AIFGLH++ + P G V SKPGPILAASGFFEAVI GKGGHAALPQHTIDPILA
Sbjct: 186 GVLDNVDAIFGLHVSSRHPFGMVISKPGPILAASGFFEAVIGGKGGHAALPQHTIDPILA 245

Query: 250 ASNIIISLQHLISREADPLDSQVVTVAKFQGGGAFNVIPDSVTIGGTFRAFSDESFIQLK 309
           ASNII+SLQHL+SREADPLDSQVVT+AKFQGGGAFNVIPDSVTIGGTFRAFS ESFIQLK
Sbjct: 246 ASNIIVSLQHLVSREADPLDSQVVTIAKFQGGGAFNVIPDSVTIGGTFRAFSKESFIQLK 305

Query: 310 QRIVEVITRQANVQRCNATVQFNE-EKPFFPVTLNDPDLYKHFHSVAGDMLGTKNILDMQ 369
           QRIVEVIT+QANVQRCNATV+FNE +KPFFPVT+ND +LYKHF ++AGDMLGTKN+LDMQ
Sbjct: 306 QRIVEVITKQANVQRCNATVEFNEDQKPFFPVTMNDQNLYKHFQTIAGDMLGTKNVLDMQ 365

Query: 370 PIMGTEDFAFYGEVIPGFFFFVGMKNETLGMFESGHSPYYIVNEDVLPYGAALHASIATR 429
           P+MGTEDF FYGEVIPG F+FVGM+NET+G F+ GHSPYYIVNEDVLPYGAAL ASIATR
Sbjct: 366 PLMGTEDFGFYGEVIPGLFYFVGMRNETVGKFDQGHSPYYIVNEDVLPYGAALQASIATR 425

Query: 430 YLL---PKSTLIKGNSHDEL 446
           YLL   PKST  KG+SHDEL
Sbjct: 426 YLLEYSPKSTSTKGHSHDEL 445

BLAST of MS003890 vs. NCBI nr
Match: XP_008450808.1 (PREDICTED: IAA-amino acid hydrolase ILR1-like 4 [Cucumis melo] >TYK10129.1 IAA-amino acid hydrolase ILR1-like 4 [Cucumis melo var. makuwa])

HSP 1 Score: 753.8 bits (1945), Expect = 8.3e-214
Identity = 373/438 (85.16%), Postives = 404/438 (92.24%), Query Frame = 0

Query: 12  NLIFIFNALLPLLIPSSSSPSPEELSQIPLNFLQYAKNPEVFNWMVDIRRQIHENPELGF 71
           +LI IF+ L+ LLIPSSSS  PEELSQIP  FLQYAK+PEVF+WMVDIRR+IHENPELGF
Sbjct: 8   SLILIFHLLISLLIPSSSSSIPEELSQIPSFFLQYAKHPEVFDWMVDIRRKIHENPELGF 67

Query: 72  EEFETSKLIRAELDRLDISYKYPVAITGVVGFIGTGNPPFVAIRADMDALALQEGVEWEH 131
           +EFETSKLIRAELDRL ISY+YPVA+TGVVGFIGTGNPPFVAIRADMDALALQEGVEWEH
Sbjct: 68  QEFETSKLIRAELDRLGISYEYPVAVTGVVGFIGTGNPPFVAIRADMDALALQEGVEWEH 127

Query: 132 KSKVPGKMHACGHDAHVAMLLGAAKILQEHRRLIQGTVVLVFQPAEEGGGGAKKMLESGV 191
           KSKVPGKMHACGHDAHVAMLLGAAKILQ++ RLIQGTVVLVFQPAEEGGGGAKK+LE+GV
Sbjct: 128 KSKVPGKMHACGHDAHVAMLLGAAKILQQNSRLIQGTVVLVFQPAEEGGGGAKKILEAGV 187

Query: 192 LDNVEAIFGLHLTGKCPIGRVASKPGPILAASGFFEAVISGKGGHAALPQHTIDPILAAS 251
           LDNV+AIFGLHL+ + P G   SKPGPILAASGFFE+VI GKGGHAALPQHTIDPILAAS
Sbjct: 188 LDNVDAIFGLHLSSRYPFGLAISKPGPILAASGFFESVIGGKGGHAALPQHTIDPILAAS 247

Query: 252 NIIISLQHLISREADPLDSQVVTVAKFQGGGAFNVIPDSVTIGGTFRAFSDESFIQLKQR 311
           NII+SLQHL+SREADPLDSQVVT+AKFQGGGAFNVIPDSVTIGGTFRAFS ESFIQLKQR
Sbjct: 248 NIIVSLQHLVSREADPLDSQVVTIAKFQGGGAFNVIPDSVTIGGTFRAFSKESFIQLKQR 307

Query: 312 IVEVITRQANVQRCNATVQFNE-EKPFFPVTLNDPDLYKHFHSVAGDMLGTKNILDMQPI 371
           IVEVIT+QA VQRCNATV+FNE +KPFFPVTLND +LYKHF +VA DMLGTKNILDMQP+
Sbjct: 308 IVEVITKQAKVQRCNATVEFNEDQKPFFPVTLNDQNLYKHFRTVAVDMLGTKNILDMQPL 367

Query: 372 MGTEDFAFYGEVIPGFFFFVGMKNETLGMFESGHSPYYIVNEDVLPYGAALHASIATRYL 431
           MG EDF FYGEVIPG F+F+GMKNET+G FE GHSPYYIVNEDVLPYGAAL ASIA RYL
Sbjct: 368 MGAEDFGFYGEVIPGLFYFLGMKNETIGKFEQGHSPYYIVNEDVLPYGAALQASIAARYL 427

Query: 432 L---PKSTLIKGNSHDEL 446
           +   PKST  KG+SHDEL
Sbjct: 428 IEYSPKSTSTKGHSHDEL 445

BLAST of MS003890 vs. NCBI nr
Match: KAG6588014.1 (IAA-amino acid hydrolase ILR1-like 4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 747.7 bits (1929), Expect = 5.9e-212
Identity = 373/438 (85.16%), Postives = 398/438 (90.87%), Query Frame = 0

Query: 12  NLIFIFNALLPLLIPSSSSPSPEELSQIPLNFLQYAKNPEVFNWMVDIRRQIHENPELGF 71
           NLIF+F+ L+   IP  SSP PEELSQIPLNFLQYAK+PEVF+WMVDIRR+IHENPELGF
Sbjct: 8   NLIFVFHVLMLCPIPLCSSPIPEELSQIPLNFLQYAKSPEVFDWMVDIRRKIHENPELGF 67

Query: 72  EEFETSKLIRAELDRLDISYKYPVAITGVVGFIGTGNPPFVAIRADMDALALQEGVEWEH 131
           EEFETSKLIRAELDRL ISY+YPVAITGVVGFIGTGNPPFVAIRADMDALALQEGV WEH
Sbjct: 68  EEFETSKLIRAELDRLGISYEYPVAITGVVGFIGTGNPPFVAIRADMDALALQEGVVWEH 127

Query: 132 KSKVPGKMHACGHDAHVAMLLGAAKILQEHRRLIQGTVVLVFQPAEEGGGGAKKMLESGV 191
           +SKVPGKMHACGHDAHVAMLLGAAKIL EHR LIQGTVVLVFQPAEEGGGGAKKMLE+GV
Sbjct: 128 QSKVPGKMHACGHDAHVAMLLGAAKILVEHRHLIQGTVVLVFQPAEEGGGGAKKMLETGV 187

Query: 192 LDNVEAIFGLHLTGKCPIGRVASKPGPILAASGFFEAVISGKGGHAALPQHTIDPILAAS 251
           L+NV+AIFG H++ K P G V S+PGPILAASGFFEAVISGKGGHAALP HT DPILAAS
Sbjct: 188 LNNVDAIFGAHVSPKYPFGMVISRPGPILAASGFFEAVISGKGGHAALPHHTFDPILAAS 247

Query: 252 NIIISLQHLISREADPLDSQVVTVAKFQGGGAFNVIPDSVTIGGTFRAFSDESFIQLKQR 311
           NIIISLQ LISRE DPLDSQVVTVAKFQGG AFNVIPDSVTIGGTFRAFS ESFIQLK R
Sbjct: 248 NIIISLQQLISRETDPLDSQVVTVAKFQGGDAFNVIPDSVTIGGTFRAFSKESFIQLKHR 307

Query: 312 IVEVITRQANVQRCNATVQFNE-EKPFFPVTLNDPDLYKHFHSVAGDMLGTKNILDMQPI 371
           IVEVIT+QANVQRCNATV+FNE +KPFFPVTLND DLY+HF +VAGDMLG+KN+LDMQP+
Sbjct: 308 IVEVITKQANVQRCNATVEFNEDQKPFFPVTLNDQDLYEHFRAVAGDMLGSKNVLDMQPL 367

Query: 372 MGTEDFAFYGEVIPGFFFFVGMKNETLGMFESGHSPYYIVNEDVLPYGAALHASIATRYL 431
           MG EDFAFY E  PGFF+FVGMKNET+G FE GHSPYYIVNEDV+PYGAAL ASIATRYL
Sbjct: 368 MGAEDFAFYCEAFPGFFYFVGMKNETVGKFEPGHSPYYIVNEDVIPYGAALQASIATRYL 427

Query: 432 L---PKSTLIKGNSHDEL 446
           L   PKST  KG SHDEL
Sbjct: 428 LEYSPKSTSTKGYSHDEL 445

BLAST of MS003890 vs. NCBI nr
Match: XP_004135685.1 (IAA-amino acid hydrolase ILR1-like 4 [Cucumis sativus] >KGN66199.1 hypothetical protein Csa_007264 [Cucumis sativus])

HSP 1 Score: 747.3 bits (1928), Expect = 7.7e-212
Identity = 372/445 (83.60%), Postives = 403/445 (90.56%), Query Frame = 0

Query: 5   MRPRRWSNLIFIFNALLPLLIPSSSSPSPEELSQIPLNFLQYAKNPEVFNWMVDIRRQIH 64
           M  R + NLI IF+ L+ LLIPSSSSP P+ELSQIP  FLQYA++PEVF+WMV IRR+IH
Sbjct: 1   MPQRSFLNLILIFHILISLLIPSSSSPIPDELSQIPSFFLQYAQHPEVFDWMVGIRRKIH 60

Query: 65  ENPELGFEEFETSKLIRAELDRLDISYKYPVAITGVVGFIGTGNPPFVAIRADMDALALQ 124
           ENPELGF+EFETSKLIRAELDRL +SY+YPVAITGVVGFIGTGNPPFVAIRADMDAL LQ
Sbjct: 61  ENPELGFQEFETSKLIRAELDRLGVSYEYPVAITGVVGFIGTGNPPFVAIRADMDALPLQ 120

Query: 125 EGVEWEHKSKVPGKMHACGHDAHVAMLLGAAKILQEHRRLIQGTVVLVFQPAEEGGGGAK 184
           EGVEWEHKSKVPGKMHACGHDAHVAMLLGAAKILQ++ RLIQGTVVLVFQPAEEGGGGAK
Sbjct: 121 EGVEWEHKSKVPGKMHACGHDAHVAMLLGAAKILQQNSRLIQGTVVLVFQPAEEGGGGAK 180

Query: 185 KMLESGVLDNVEAIFGLHLTGKCPIGRVASKPGPILAASGFFEAVISGKGGHAALPQHTI 244
           KMLE+GVLD V+AIFGLH++   P G   SKPGPILAASGFFEAVI GKGGHAALPQHTI
Sbjct: 181 KMLEAGVLDKVDAIFGLHVSSSYPFGMAISKPGPILAASGFFEAVIGGKGGHAALPQHTI 240

Query: 245 DPILAASNIIISLQHLISREADPLDSQVVTVAKFQGGGAFNVIPDSVTIGGTFRAFSDES 304
           DPILAASNII+SLQHL+SREADPLDSQVVT+AKFQGGGAFNVIPDSVTIGGTFRAFS ES
Sbjct: 241 DPILAASNIIVSLQHLVSREADPLDSQVVTIAKFQGGGAFNVIPDSVTIGGTFRAFSKES 300

Query: 305 FIQLKQRIVEVITRQANVQRCNATVQFNE-EKPFFPVTLNDPDLYKHFHSVAGDMLGTKN 364
           FIQLKQRIVEVIT QA VQRCNATV+FNE +KPFFPVT+N+ +LYKHF +VA DMLGTKN
Sbjct: 301 FIQLKQRIVEVITNQAKVQRCNATVEFNEDQKPFFPVTVNNHNLYKHFRTVAVDMLGTKN 360

Query: 365 ILDMQPIMGTEDFAFYGEVIPGFFFFVGMKNETLGMFESGHSPYYIVNEDVLPYGAALHA 424
           ILDMQP+MG EDF FYGEVIPG F+F+GMKNET+G FE GHSPYYIVNEDVLPYGAAL A
Sbjct: 361 ILDMQPLMGAEDFGFYGEVIPGLFYFLGMKNETVGKFEQGHSPYYIVNEDVLPYGAALQA 420

Query: 425 SIATRYLL---PKSTLIKGNSHDEL 446
           SIATRYLL   PKST  K +SHDEL
Sbjct: 421 SIATRYLLEHSPKSTSTKEHSHDEL 445

BLAST of MS003890 vs. ExPASy Swiss-Prot
Match: O04373 (IAA-amino acid hydrolase ILR1-like 4 OS=Arabidopsis thaliana OX=3702 GN=ILL4 PE=1 SV=2)

HSP 1 Score: 622.5 bits (1604), Expect = 3.8e-177
Identity = 305/440 (69.32%), Postives = 368/440 (83.64%), Query Frame = 0

Query: 9   RWSNLIFIFNALLPLLIPSSSSPSPEELSQIPLNFLQYAKNPEVFNWMVDIRRQIHENPE 68
           +W + + I + L P LI  SS+     LSQIP  FL  AK  + F+WMV IRR+IHENPE
Sbjct: 5   KWVSFVLILHLLNPTLISCSSN----GLSQIPSKFLTLAKRNDFFDWMVGIRRRIHENPE 64

Query: 69  LGFEEFETSKLIRAELDRLDISYKYPVAITGVVGFIGTGNPPFVAIRADMDALALQEGVE 128
           LG+EE ETSKL+RAEL+++ +SYKYPVA+TGVVG++GTG+ PFVA+RADMDALA+QE VE
Sbjct: 65  LGYEEVETSKLVRAELEKMGVSYKYPVAVTGVVGYVGTGHAPFVALRADMDALAMQEMVE 124

Query: 129 WEHKSKVPGKMHACGHDAHVAMLLGAAKILQEHRRLIQGTVVLVFQPAEEGGGGAKKMLE 188
           WEHKSKVPGKMHACGHDAH  MLLGAAK+L+EH   +QGTVVLVFQPAEEGGGGAKK++E
Sbjct: 125 WEHKSKVPGKMHACGHDAHTTMLLGAAKLLKEHEEELQGTVVLVFQPAEEGGGGAKKIVE 184

Query: 189 SGVLDNVEAIFGLHLTGKCPIGRVASKPGPILAASGFFEAVISGKGGHAALPQHTIDPIL 248
           +GVL+NV AIFGLH+T +  +G+V+S+ GP+LA SGFF+A ISGKGGHAALPQHTIDPIL
Sbjct: 185 AGVLENVSAIFGLHVTNQLALGQVSSREGPMLAGSGFFKAKISGKGGHAALPQHTIDPIL 244

Query: 249 AASNIIISLQHLISREADPLDSQVVTVAKFQGGGAFNVIPDSVTIGGTFRAFSDESFIQL 308
           AASN+I+SLQHL+SREADPLDSQVVTVAKF+GGGAFNVIPDSVTIGGTFRAFS +SF+QL
Sbjct: 245 AASNVIVSLQHLVSREADPLDSQVVTVAKFEGGGAFNVIPDSVTIGGTFRAFSTKSFMQL 304

Query: 309 KQRIVEVITRQANVQRCNATVQF-NEEKPFFPVTLNDPDLYKHFHSVAGDMLGTKNILDM 368
           K+RI +VITRQA+V  CNATV F  EEKPFFP T+ND  L++ F +V+GDMLG +N ++M
Sbjct: 305 KKRIEQVITRQASVNMCNATVDFIEEEKPFFPPTVNDKALHQFFKNVSGDMLGIENYVEM 364

Query: 369 QPIMGTEDFAFYGEVIPGFFFFVGMKNETLGMFESGHSPYYIVNEDVLPYGAALHASIAT 428
           QP+MG+EDF+FY + IPG F FVGM+N+      S HSPY+ VNE++LPYGA+LHAS+AT
Sbjct: 365 QPLMGSEDFSFYQQAIPGHFSFVGMQNKARSPMASPHSPYFEVNEELLPYGASLHASMAT 424

Query: 429 RYL--LPKSTLIKGNSHDEL 446
           RYL  L  STL K N  DEL
Sbjct: 425 RYLLELKASTLNKSNKKDEL 440

BLAST of MS003890 vs. ExPASy Swiss-Prot
Match: Q84XG9 (IAA-amino acid hydrolase ILR1-like 1 OS=Oryza sativa subsp. indica OX=39946 GN=ILL1 PE=2 SV=1)

HSP 1 Score: 576.6 bits (1485), Expect = 2.4e-163
Identity = 281/410 (68.54%), Postives = 330/410 (80.49%), Query Frame = 0

Query: 40  PLNFLQYAKNPEVFNWMVDIRRQIHENPELGFEEFETSKLIRAELDRLDISYKYPVAITG 99
           P   L+ AK  E   WMV +RR+IHENPELG+EEF TS+L+R ELD L I Y++P A+TG
Sbjct: 33  PAGLLRRAKEAEFAGWMVGLRRRIHENPELGYEEFATSELVRRELDALGIPYRHPFAVTG 92

Query: 100 VVGFIGTGNPPFVAIRADMDALALQEGVEWEHKSKVPGKMHACGHDAHVAMLLGAAKILQ 159
           VV  +GTG PPFVA+RADMDAL +QE VEWEHKSKVPGKMH CGHDAHVAMLLG+A+ILQ
Sbjct: 93  VVATVGTGGPPFVALRADMDALPMQESVEWEHKSKVPGKMHGCGHDAHVAMLLGSARILQ 152

Query: 160 EHRRLIQGTVVLVFQPAEEGGGGAKKMLESGVLDNVEAIFGLHLTGKCPIGRVASKPGPI 219
           EHR  ++GTVVLVFQPAEEGGGGAKKM++ G ++N+EAIFG+H+    PIG VAS+PGP+
Sbjct: 153 EHRDELKGTVVLVFQPAEEGGGGAKKMIDDGTVENIEAIFGVHVADVVPIGVVASRPGPV 212

Query: 220 LAASGFFEAVISGKGGHAALPQHTIDPILAASNIIISLQHLISREADPLDSQVVTVAKFQ 279
           +A SGFFEAVISGKGGHAALP HTIDPILAASN+I+SLQ L+SREADPLDSQVVTV KFQ
Sbjct: 213 MAGSGFFEAVISGKGGHAALPHHTIDPILAASNVIVSLQQLVSREADPLDSQVVTVGKFQ 272

Query: 280 GGGAFNVIPDSVTIGGTFRAFSDESFIQLKQRIVEVITRQANVQRCNATVQF-NEEKPFF 339
           GGGAFNVIPDSVTIGGTFRAF  ESF QLKQRI EVI  QA+VQRCNA V F ++++PFF
Sbjct: 273 GGGAFNVIPDSVTIGGTFRAFLKESFNQLKQRIEEVIVSQASVQRCNAVVDFLDKDRPFF 332

Query: 340 PVTLNDPDLYKHFHSVAGDMLGTKNILDMQPIMGTEDFAFYGEVIPG-FFFFVGMKNETL 399
           P T+N   L+  F  VA +M+G KN+ D QP+MG EDFAFY + IP  +++F+GM NET 
Sbjct: 333 PPTINSAGLHDFFVKVASEMVGPKNVRDKQPLMGAEDFAFYADAIPATYYYFLGMYNETR 392

Query: 400 GMFESGHSPYYIVNEDVLPYGAALHASIATRYLLPKS--TLIKGNSHDEL 446
           G     HSPY+ +NED LPYGAAL AS+ATRYLL     T  K  +HDEL
Sbjct: 393 GPQAPHHSPYFTINEDALPYGAALQASLATRYLLEHQPPTTGKAKAHDEL 442

BLAST of MS003890 vs. ExPASy Swiss-Prot
Match: Q8S9S4 (IAA-amino acid hydrolase ILR1-like 1 OS=Oryza sativa subsp. japonica OX=39947 GN=ILL1 PE=2 SV=1)

HSP 1 Score: 574.7 bits (1480), Expect = 9.0e-163
Identity = 280/410 (68.29%), Postives = 329/410 (80.24%), Query Frame = 0

Query: 40  PLNFLQYAKNPEVFNWMVDIRRQIHENPELGFEEFETSKLIRAELDRLDISYKYPVAITG 99
           P   L+ AK  E   WMV +RR+IHENPELG+EEF TS+L+R ELD L I Y++P A+TG
Sbjct: 33  PAGLLRRAKEAEFAGWMVGLRRRIHENPELGYEEFATSELVRRELDALGIPYRHPFAVTG 92

Query: 100 VVGFIGTGNPPFVAIRADMDALALQEGVEWEHKSKVPGKMHACGHDAHVAMLLGAAKILQ 159
           VV  +GTG PPFVA+RADMDAL +QE VEWEHKSKVPGKMH CGHDAHVAMLLG+A+ILQ
Sbjct: 93  VVATVGTGGPPFVALRADMDALPMQESVEWEHKSKVPGKMHGCGHDAHVAMLLGSARILQ 152

Query: 160 EHRRLIQGTVVLVFQPAEEGGGGAKKMLESGVLDNVEAIFGLHLTGKCPIGRVASKPGPI 219
           EHR  ++GTVVLVFQPAEEGGGGAKKM++ G ++N+EAIFG+H+    PIG VAS+PGP+
Sbjct: 153 EHRDELKGTVVLVFQPAEEGGGGAKKMIDDGAVENIEAIFGVHVADVVPIGVVASRPGPV 212

Query: 220 LAASGFFEAVISGKGGHAALPQHTIDPILAASNIIISLQHLISREADPLDSQVVTVAKFQ 279
           +A SGFFEAVISGKGGHAALP HTIDPILAASN+I+SLQ L+SREADPLDSQVVTV KFQ
Sbjct: 213 MAGSGFFEAVISGKGGHAALPHHTIDPILAASNVIVSLQQLVSREADPLDSQVVTVGKFQ 272

Query: 280 GGGAFNVIPDSVTIGGTFRAFSDESFIQLKQRIVEVITRQANVQRCNATVQF-NEEKPFF 339
           GGGAFNVIPDSVTIGGTFRAF  ESF QLKQRI EVI  QA+VQRCNA V F ++++PFF
Sbjct: 273 GGGAFNVIPDSVTIGGTFRAFLKESFNQLKQRIEEVIVSQASVQRCNAVVDFLDKDRPFF 332

Query: 340 PVTLNDPDLYKHFHSVAGDMLGTKNILDMQPIMGTEDFAFYGEVIPG-FFFFVGMKNETL 399
           P T+N   L+  F  VA +M+G KN+ D QP+MG EDFAFY + IP  +++F+GM NET 
Sbjct: 333 PPTINSAGLHDFFVKVASEMVGPKNVRDKQPLMGAEDFAFYADAIPATYYYFLGMYNETR 392

Query: 400 GMFESGHSPYYIVNEDVLPYGAALHASIATRYLLPKS--TLIKGNSHDEL 446
           G     HSPY+ +NED LPYGAAL AS+A RYLL     T  K  +HDEL
Sbjct: 393 GPQAPHHSPYFTINEDALPYGAALQASLAARYLLEHQPPTTGKAKAHDEL 442

BLAST of MS003890 vs. ExPASy Swiss-Prot
Match: P54970 (IAA-amino acid hydrolase ILR1-like 2 OS=Arabidopsis thaliana OX=3702 GN=ILL2 PE=1 SV=2)

HSP 1 Score: 549.3 bits (1414), Expect = 4.1e-155
Identity = 277/439 (63.10%), Postives = 341/439 (77.68%), Query Frame = 0

Query: 13  LIFIFNALLPLLIPSSSSP-SPEELSQIPLNFLQYAKNPEVFNWMVDIRRQIHENPELGF 72
           L   F  LL LL  SS SP   E+ SQI    L++AK+PEVF+WMV IRR+IHENPELG+
Sbjct: 7   LSLTFQLLLFLLSVSSESPWIAEDTSQIQTKLLEFAKSPEVFDWMVKIRRKIHENPELGY 66

Query: 73  EEFETSKLIRAELDRLDISYKYPVAITGVVGFIGTGNPPFVAIRADMDALALQEGVEWEH 132
           EE ETSKLIR+EL+ + I Y+YPVAITGV+G+IGTG PPFVA+RADMDAL +QEGVEWEH
Sbjct: 67  EELETSKLIRSELELIGIKYRYPVAITGVIGYIGTGEPPFVALRADMDALPIQEGVEWEH 126

Query: 133 KSKVPGKMHACGHDAHVAMLLGAAKILQEHRRLIQGTVVLVFQPAEEGGGGAKKMLESGV 192
           KSK+ GKMHACGHD HV MLLGAAKIL EHR  +QGTVVL+FQPAEEG  GAKKM E G 
Sbjct: 127 KSKIAGKMHACGHDGHVTMLLGAAKILHEHRHHLQGTVVLIFQPAEEGLSGAKKMREEGA 186

Query: 193 LDNVEAIFGLHLTGKCPIGRVASKPGPILAASGFFEAVISGKGGHAALPQHTIDPILAAS 252
           L NVEAIFG+HL+ + P G+ AS+ G  LA +G FEAVI+GKGGHAA+PQHTIDP++AAS
Sbjct: 187 LKNVEAIFGIHLSARIPFGKAASRAGSFLAGAGVFEAVITGKGGHAAIPQHTIDPVVAAS 246

Query: 253 NIIISLQHLISREADPLDSQVVTVAKFQGGGAFNVIPDSVTIGGTFRAFSDESFIQLKQR 312
           +I++SLQ L+SRE DPLDS+VVTV+K  GG AFNVIPDS+TIGGT RAF+   F QL+QR
Sbjct: 247 SIVLSLQQLVSRETDPLDSKVVTVSKVNGGNAFNVIPDSITIGGTLRAFT--GFTQLQQR 306

Query: 313 IVEVITRQANVQRCNATVQF--NEEKPFFPVTLNDPDLYKHFHSVAGDMLGTKNILDMQP 372
           + EVIT+QA V RCNA+V    N  +P  P T+N+ DLYK F  V  D+LG +  ++  P
Sbjct: 307 VKEVITKQAAVHRCNASVNLTPNGREP-MPPTVNNKDLYKQFKKVVRDLLGQEAFVEAAP 366

Query: 373 IMGTEDFAFYGEVIPGFFFFVGMKNETLGMFESGHSPYYIVNEDVLPYGAALHASIATRY 432
           +MG+EDF+++ E IPG F  +GM++ET G + S HSP Y +NEDVLPYGAA+HAS+A +Y
Sbjct: 367 VMGSEDFSYFAETIPGHFSLLGMQDETNG-YASSHSPLYRINEDVLPYGAAIHASMAVQY 426

Query: 433 LLPKSTLIKGN---SHDEL 446
           L  K++  KG+    H+EL
Sbjct: 427 LKEKAS--KGSVSGFHEEL 439

BLAST of MS003890 vs. ExPASy Swiss-Prot
Match: Q9SWX9 (IAA-amino acid hydrolase ILR1-like 5 OS=Arabidopsis thaliana OX=3702 GN=ILL5 PE=3 SV=1)

HSP 1 Score: 545.8 bits (1405), Expect = 4.5e-154
Identity = 267/420 (63.57%), Postives = 337/420 (80.24%), Query Frame = 0

Query: 17  FNALLPLLIPSSSSPSPEELSQIPLNFLQYAKNPEVFNWMVDIRRQIHENPELGFEEFET 76
           F  +L LL     S S  +LSQIP NFL  AK  + F+WMV IRR+IHENPELG+EE ET
Sbjct: 9   FVLILHLLNSCLISCSSNDLSQIPKNFLSLAKREDFFDWMVGIRRRIHENPELGYEEVET 68

Query: 77  SKLIRAELDRLDISYKYPVAITGVVGFIGTGNPPFVAIRADMDALALQEGVEWEHKSKVP 136
           SKL++ ELD++ +SYK PVA+TGV+G++GTG+ PFVA+RADMDAL +QE VEWEHKSK+P
Sbjct: 69  SKLVKTELDKMGVSYKNPVAVTGVIGYVGTGHAPFVALRADMDALPIQEMVEWEHKSKIP 128

Query: 137 GKMHACGHDAHVAMLLGAAKILQEHRRLIQGTVVLVFQPAEEGGGGAKKMLESGVLDNVE 196
           GKMHACGHDAH  MLLGAAK+L+EH+  +QGTV+LVFQPAEEGG GAKK++E+GVL+NV 
Sbjct: 129 GKMHACGHDAHTTMLLGAAKLLKEHQEELQGTVILVFQPAEEGGAGAKKIVEAGVLENVG 188

Query: 197 AIFGLHLTGKCPIGRVASKPGPILAASGFFEAVISGKGGHAALPQHTIDPILAASNIIIS 256
           AIFGLH++    +G+++S+ G ++A SG F+A ISGKGGHAALPQ  IDP+LAASN+I+S
Sbjct: 189 AIFGLHVSNLLGLGQLSSREGLLMAGSGRFKATISGKGGHAALPQFAIDPVLAASNVILS 248

Query: 257 LQHLISREADPLDSQVVTVAKFQGGGAFNVIPDSVTIGGTFRAFSDESFIQLKQRIVEVI 316
           LQHL+SREADPLDSQVVTVA F+G  AFNVIPDSVTIGGTFRA   +SF QLKQRIV+VI
Sbjct: 249 LQHLVSREADPLDSQVVTVATFEGSDAFNVIPDSVTIGGTFRALLPKSFEQLKQRIVQVI 308

Query: 317 TRQANVQRCNATVQF-NEEKPFFPVTLNDPDLYKHFHSVAGDMLGTKNILDMQPIMGTED 376
           T QA+V  CNATV F  +E P FP T+N+  L+  + +V+ DMLG +N ++  P+M +ED
Sbjct: 309 TTQASVNMCNATVDFLEDETPPFPPTVNNKTLHLFYKNVSVDMLGIENYVETLPVMVSED 368

Query: 377 FAFYGEVIPGFFFFVGMKNETLGMFESGHSPYYIVNEDVLPYGAALHASIATRYLLPKST 436
           FAFY + IPG F FVGM+N++     + HSP++ VNE++LPYGA+L AS+ATRYLL  S+
Sbjct: 369 FAFYQQAIPGHFSFVGMQNKSHSPMANPHSPFFEVNEELLPYGASLLASLATRYLLDSSS 428

BLAST of MS003890 vs. ExPASy TrEMBL
Match: A0A6J1CTB6 (IAA-amino acid hydrolase ILR1-like 4 OS=Momordica charantia OX=3673 GN=LOC111014558 PE=3 SV=1)

HSP 1 Score: 891.7 bits (2303), Expect = 1.2e-255
Identity = 441/441 (100.00%), Postives = 441/441 (100.00%), Query Frame = 0

Query: 5   MRPRRWSNLIFIFNALLPLLIPSSSSPSPEELSQIPLNFLQYAKNPEVFNWMVDIRRQIH 64
           MRPRRWSNLIFIFNALLPLLIPSSSSPSPEELSQIPLNFLQYAKNPEVFNWMVDIRRQIH
Sbjct: 1   MRPRRWSNLIFIFNALLPLLIPSSSSPSPEELSQIPLNFLQYAKNPEVFNWMVDIRRQIH 60

Query: 65  ENPELGFEEFETSKLIRAELDRLDISYKYPVAITGVVGFIGTGNPPFVAIRADMDALALQ 124
           ENPELGFEEFETSKLIRAELDRLDISYKYPVAITGVVGFIGTGNPPFVAIRADMDALALQ
Sbjct: 61  ENPELGFEEFETSKLIRAELDRLDISYKYPVAITGVVGFIGTGNPPFVAIRADMDALALQ 120

Query: 125 EGVEWEHKSKVPGKMHACGHDAHVAMLLGAAKILQEHRRLIQGTVVLVFQPAEEGGGGAK 184
           EGVEWEHKSKVPGKMHACGHDAHVAMLLGAAKILQEHRRLIQGTVVLVFQPAEEGGGGAK
Sbjct: 121 EGVEWEHKSKVPGKMHACGHDAHVAMLLGAAKILQEHRRLIQGTVVLVFQPAEEGGGGAK 180

Query: 185 KMLESGVLDNVEAIFGLHLTGKCPIGRVASKPGPILAASGFFEAVISGKGGHAALPQHTI 244
           KMLESGVLDNVEAIFGLHLTGKCPIGRVASKPGPILAASGFFEAVISGKGGHAALPQHTI
Sbjct: 181 KMLESGVLDNVEAIFGLHLTGKCPIGRVASKPGPILAASGFFEAVISGKGGHAALPQHTI 240

Query: 245 DPILAASNIIISLQHLISREADPLDSQVVTVAKFQGGGAFNVIPDSVTIGGTFRAFSDES 304
           DPILAASNIIISLQHLISREADPLDSQVVTVAKFQGGGAFNVIPDSVTIGGTFRAFSDES
Sbjct: 241 DPILAASNIIISLQHLISREADPLDSQVVTVAKFQGGGAFNVIPDSVTIGGTFRAFSDES 300

Query: 305 FIQLKQRIVEVITRQANVQRCNATVQFNEEKPFFPVTLNDPDLYKHFHSVAGDMLGTKNI 364
           FIQLKQRIVEVITRQANVQRCNATVQFNEEKPFFPVTLNDPDLYKHFHSVAGDMLGTKNI
Sbjct: 301 FIQLKQRIVEVITRQANVQRCNATVQFNEEKPFFPVTLNDPDLYKHFHSVAGDMLGTKNI 360

Query: 365 LDMQPIMGTEDFAFYGEVIPGFFFFVGMKNETLGMFESGHSPYYIVNEDVLPYGAALHAS 424
           LDMQPIMGTEDFAFYGEVIPGFFFFVGMKNETLGMFESGHSPYYIVNEDVLPYGAALHAS
Sbjct: 361 LDMQPIMGTEDFAFYGEVIPGFFFFVGMKNETLGMFESGHSPYYIVNEDVLPYGAALHAS 420

Query: 425 IATRYLLPKSTLIKGNSHDEL 446
           IATRYLLPKSTLIKGNSHDEL
Sbjct: 421 IATRYLLPKSTLIKGNSHDEL 441

BLAST of MS003890 vs. ExPASy TrEMBL
Match: A0A5D3CIQ7 (IAA-amino acid hydrolase ILR1-like 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G002720 PE=3 SV=1)

HSP 1 Score: 753.8 bits (1945), Expect = 4.0e-214
Identity = 373/438 (85.16%), Postives = 404/438 (92.24%), Query Frame = 0

Query: 12  NLIFIFNALLPLLIPSSSSPSPEELSQIPLNFLQYAKNPEVFNWMVDIRRQIHENPELGF 71
           +LI IF+ L+ LLIPSSSS  PEELSQIP  FLQYAK+PEVF+WMVDIRR+IHENPELGF
Sbjct: 8   SLILIFHLLISLLIPSSSSSIPEELSQIPSFFLQYAKHPEVFDWMVDIRRKIHENPELGF 67

Query: 72  EEFETSKLIRAELDRLDISYKYPVAITGVVGFIGTGNPPFVAIRADMDALALQEGVEWEH 131
           +EFETSKLIRAELDRL ISY+YPVA+TGVVGFIGTGNPPFVAIRADMDALALQEGVEWEH
Sbjct: 68  QEFETSKLIRAELDRLGISYEYPVAVTGVVGFIGTGNPPFVAIRADMDALALQEGVEWEH 127

Query: 132 KSKVPGKMHACGHDAHVAMLLGAAKILQEHRRLIQGTVVLVFQPAEEGGGGAKKMLESGV 191
           KSKVPGKMHACGHDAHVAMLLGAAKILQ++ RLIQGTVVLVFQPAEEGGGGAKK+LE+GV
Sbjct: 128 KSKVPGKMHACGHDAHVAMLLGAAKILQQNSRLIQGTVVLVFQPAEEGGGGAKKILEAGV 187

Query: 192 LDNVEAIFGLHLTGKCPIGRVASKPGPILAASGFFEAVISGKGGHAALPQHTIDPILAAS 251
           LDNV+AIFGLHL+ + P G   SKPGPILAASGFFE+VI GKGGHAALPQHTIDPILAAS
Sbjct: 188 LDNVDAIFGLHLSSRYPFGLAISKPGPILAASGFFESVIGGKGGHAALPQHTIDPILAAS 247

Query: 252 NIIISLQHLISREADPLDSQVVTVAKFQGGGAFNVIPDSVTIGGTFRAFSDESFIQLKQR 311
           NII+SLQHL+SREADPLDSQVVT+AKFQGGGAFNVIPDSVTIGGTFRAFS ESFIQLKQR
Sbjct: 248 NIIVSLQHLVSREADPLDSQVVTIAKFQGGGAFNVIPDSVTIGGTFRAFSKESFIQLKQR 307

Query: 312 IVEVITRQANVQRCNATVQFNE-EKPFFPVTLNDPDLYKHFHSVAGDMLGTKNILDMQPI 371
           IVEVIT+QA VQRCNATV+FNE +KPFFPVTLND +LYKHF +VA DMLGTKNILDMQP+
Sbjct: 308 IVEVITKQAKVQRCNATVEFNEDQKPFFPVTLNDQNLYKHFRTVAVDMLGTKNILDMQPL 367

Query: 372 MGTEDFAFYGEVIPGFFFFVGMKNETLGMFESGHSPYYIVNEDVLPYGAALHASIATRYL 431
           MG EDF FYGEVIPG F+F+GMKNET+G FE GHSPYYIVNEDVLPYGAAL ASIA RYL
Sbjct: 368 MGAEDFGFYGEVIPGLFYFLGMKNETIGKFEQGHSPYYIVNEDVLPYGAALQASIAARYL 427

Query: 432 L---PKSTLIKGNSHDEL 446
           +   PKST  KG+SHDEL
Sbjct: 428 IEYSPKSTSTKGHSHDEL 445

BLAST of MS003890 vs. ExPASy TrEMBL
Match: A0A1S3BQ36 (IAA-amino acid hydrolase ILR1-like 4 OS=Cucumis melo OX=3656 GN=LOC103492283 PE=3 SV=1)

HSP 1 Score: 753.8 bits (1945), Expect = 4.0e-214
Identity = 373/438 (85.16%), Postives = 404/438 (92.24%), Query Frame = 0

Query: 12  NLIFIFNALLPLLIPSSSSPSPEELSQIPLNFLQYAKNPEVFNWMVDIRRQIHENPELGF 71
           +LI IF+ L+ LLIPSSSS  PEELSQIP  FLQYAK+PEVF+WMVDIRR+IHENPELGF
Sbjct: 8   SLILIFHLLISLLIPSSSSSIPEELSQIPSFFLQYAKHPEVFDWMVDIRRKIHENPELGF 67

Query: 72  EEFETSKLIRAELDRLDISYKYPVAITGVVGFIGTGNPPFVAIRADMDALALQEGVEWEH 131
           +EFETSKLIRAELDRL ISY+YPVA+TGVVGFIGTGNPPFVAIRADMDALALQEGVEWEH
Sbjct: 68  QEFETSKLIRAELDRLGISYEYPVAVTGVVGFIGTGNPPFVAIRADMDALALQEGVEWEH 127

Query: 132 KSKVPGKMHACGHDAHVAMLLGAAKILQEHRRLIQGTVVLVFQPAEEGGGGAKKMLESGV 191
           KSKVPGKMHACGHDAHVAMLLGAAKILQ++ RLIQGTVVLVFQPAEEGGGGAKK+LE+GV
Sbjct: 128 KSKVPGKMHACGHDAHVAMLLGAAKILQQNSRLIQGTVVLVFQPAEEGGGGAKKILEAGV 187

Query: 192 LDNVEAIFGLHLTGKCPIGRVASKPGPILAASGFFEAVISGKGGHAALPQHTIDPILAAS 251
           LDNV+AIFGLHL+ + P G   SKPGPILAASGFFE+VI GKGGHAALPQHTIDPILAAS
Sbjct: 188 LDNVDAIFGLHLSSRYPFGLAISKPGPILAASGFFESVIGGKGGHAALPQHTIDPILAAS 247

Query: 252 NIIISLQHLISREADPLDSQVVTVAKFQGGGAFNVIPDSVTIGGTFRAFSDESFIQLKQR 311
           NII+SLQHL+SREADPLDSQVVT+AKFQGGGAFNVIPDSVTIGGTFRAFS ESFIQLKQR
Sbjct: 248 NIIVSLQHLVSREADPLDSQVVTIAKFQGGGAFNVIPDSVTIGGTFRAFSKESFIQLKQR 307

Query: 312 IVEVITRQANVQRCNATVQFNE-EKPFFPVTLNDPDLYKHFHSVAGDMLGTKNILDMQPI 371
           IVEVIT+QA VQRCNATV+FNE +KPFFPVTLND +LYKHF +VA DMLGTKNILDMQP+
Sbjct: 308 IVEVITKQAKVQRCNATVEFNEDQKPFFPVTLNDQNLYKHFRTVAVDMLGTKNILDMQPL 367

Query: 372 MGTEDFAFYGEVIPGFFFFVGMKNETLGMFESGHSPYYIVNEDVLPYGAALHASIATRYL 431
           MG EDF FYGEVIPG F+F+GMKNET+G FE GHSPYYIVNEDVLPYGAAL ASIA RYL
Sbjct: 368 MGAEDFGFYGEVIPGLFYFLGMKNETIGKFEQGHSPYYIVNEDVLPYGAALQASIAARYL 427

Query: 432 L---PKSTLIKGNSHDEL 446
           +   PKST  KG+SHDEL
Sbjct: 428 IEYSPKSTSTKGHSHDEL 445

BLAST of MS003890 vs. ExPASy TrEMBL
Match: A0A0A0LYS1 (M20_dimer domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G575150 PE=3 SV=1)

HSP 1 Score: 747.3 bits (1928), Expect = 3.7e-212
Identity = 372/445 (83.60%), Postives = 403/445 (90.56%), Query Frame = 0

Query: 5   MRPRRWSNLIFIFNALLPLLIPSSSSPSPEELSQIPLNFLQYAKNPEVFNWMVDIRRQIH 64
           M  R + NLI IF+ L+ LLIPSSSSP P+ELSQIP  FLQYA++PEVF+WMV IRR+IH
Sbjct: 1   MPQRSFLNLILIFHILISLLIPSSSSPIPDELSQIPSFFLQYAQHPEVFDWMVGIRRKIH 60

Query: 65  ENPELGFEEFETSKLIRAELDRLDISYKYPVAITGVVGFIGTGNPPFVAIRADMDALALQ 124
           ENPELGF+EFETSKLIRAELDRL +SY+YPVAITGVVGFIGTGNPPFVAIRADMDAL LQ
Sbjct: 61  ENPELGFQEFETSKLIRAELDRLGVSYEYPVAITGVVGFIGTGNPPFVAIRADMDALPLQ 120

Query: 125 EGVEWEHKSKVPGKMHACGHDAHVAMLLGAAKILQEHRRLIQGTVVLVFQPAEEGGGGAK 184
           EGVEWEHKSKVPGKMHACGHDAHVAMLLGAAKILQ++ RLIQGTVVLVFQPAEEGGGGAK
Sbjct: 121 EGVEWEHKSKVPGKMHACGHDAHVAMLLGAAKILQQNSRLIQGTVVLVFQPAEEGGGGAK 180

Query: 185 KMLESGVLDNVEAIFGLHLTGKCPIGRVASKPGPILAASGFFEAVISGKGGHAALPQHTI 244
           KMLE+GVLD V+AIFGLH++   P G   SKPGPILAASGFFEAVI GKGGHAALPQHTI
Sbjct: 181 KMLEAGVLDKVDAIFGLHVSSSYPFGMAISKPGPILAASGFFEAVIGGKGGHAALPQHTI 240

Query: 245 DPILAASNIIISLQHLISREADPLDSQVVTVAKFQGGGAFNVIPDSVTIGGTFRAFSDES 304
           DPILAASNII+SLQHL+SREADPLDSQVVT+AKFQGGGAFNVIPDSVTIGGTFRAFS ES
Sbjct: 241 DPILAASNIIVSLQHLVSREADPLDSQVVTIAKFQGGGAFNVIPDSVTIGGTFRAFSKES 300

Query: 305 FIQLKQRIVEVITRQANVQRCNATVQFNE-EKPFFPVTLNDPDLYKHFHSVAGDMLGTKN 364
           FIQLKQRIVEVIT QA VQRCNATV+FNE +KPFFPVT+N+ +LYKHF +VA DMLGTKN
Sbjct: 301 FIQLKQRIVEVITNQAKVQRCNATVEFNEDQKPFFPVTVNNHNLYKHFRTVAVDMLGTKN 360

Query: 365 ILDMQPIMGTEDFAFYGEVIPGFFFFVGMKNETLGMFESGHSPYYIVNEDVLPYGAALHA 424
           ILDMQP+MG EDF FYGEVIPG F+F+GMKNET+G FE GHSPYYIVNEDVLPYGAAL A
Sbjct: 361 ILDMQPLMGAEDFGFYGEVIPGLFYFLGMKNETVGKFEQGHSPYYIVNEDVLPYGAALQA 420

Query: 425 SIATRYLL---PKSTLIKGNSHDEL 446
           SIATRYLL   PKST  K +SHDEL
Sbjct: 421 SIATRYLLEHSPKSTSTKEHSHDEL 445

BLAST of MS003890 vs. ExPASy TrEMBL
Match: A0A6J1F5X7 (IAA-amino acid hydrolase ILR1-like 4 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111441127 PE=3 SV=1)

HSP 1 Score: 740.0 bits (1909), Expect = 6.0e-210
Identity = 370/438 (84.47%), Postives = 396/438 (90.41%), Query Frame = 0

Query: 12  NLIFIFNALLPLLIPSSSSPSPEELSQIPLNFLQYAKNPEVFNWMVDIRRQIHENPELGF 71
           NLIF+F+ L+   IP  SSP PEELSQIPLNFLQYAK+PEVF+WMVDIRR+IHENPELGF
Sbjct: 8   NLIFVFHVLMLCPIPLCSSPIPEELSQIPLNFLQYAKSPEVFDWMVDIRRKIHENPELGF 67

Query: 72  EEFETSKLIRAELDRLDISYKYPVAITGVVGFIGTGNPPFVAIRADMDALALQEGVEWEH 131
           EEF+TSKLIRAELDRL ISY+YPVAITGVVGFIGTGNPPFVAIRADMDALALQEGV WEH
Sbjct: 68  EEFQTSKLIRAELDRLGISYEYPVAITGVVGFIGTGNPPFVAIRADMDALALQEGVVWEH 127

Query: 132 KSKVPGKMHACGHDAHVAMLLGAAKILQEHRRLIQGTVVLVFQPAEEGGGGAKKMLESGV 191
           +SKVPGKMHACGHDAHVAMLLGAAKIL EHR LIQGTVVLVFQPAEEGGGGAKKMLE+GV
Sbjct: 128 QSKVPGKMHACGHDAHVAMLLGAAKILVEHRHLIQGTVVLVFQPAEEGGGGAKKMLETGV 187

Query: 192 LDNVEAIFGLHLTGKCPIGRVASKPGPILAASGFFEAVISGKGGHAALPQHTIDPILAAS 251
           L+NV+AIFG H++ K P G V S+PGPILAASGFFEAVISGKGGHAALP HT DPILAAS
Sbjct: 188 LNNVDAIFGAHVSPKYPFGMVISRPGPILAASGFFEAVISGKGGHAALPHHTFDPILAAS 247

Query: 252 NIIISLQHLISREADPLDSQVVTVAKFQGGGAFNVIPDSVTIGGTFRAFSDESFIQLKQR 311
           NIIISLQ LISRE DPLDSQVVTVAKFQGG AFNVIPDSVTIGGTFRAFS ESFIQLK R
Sbjct: 248 NIIISLQQLISRETDPLDSQVVTVAKFQGGDAFNVIPDSVTIGGTFRAFSRESFIQLKHR 307

Query: 312 IVEVITRQANVQRCNATVQFNE-EKPFFPVTLNDPDLYKHFHSVAGDMLGTKNILDMQPI 371
           IVEVIT+QANVQRCNATV+FNE +KP FPVTLND DLY+HF +VAGDMLG+KN+LDMQP+
Sbjct: 308 IVEVITKQANVQRCNATVEFNEDQKPSFPVTLNDQDLYEHFRAVAGDMLGSKNVLDMQPL 367

Query: 372 MGTEDFAFYGEVIPGFFFFVGMKNETLGMFESGHSPYYIVNEDVLPYGAALHASIATRYL 431
           MG EDFAFY E  PGFF+FVGMKNET+G FE GHSPYYIVNEDV+PYGAAL ASIATRYL
Sbjct: 368 MGAEDFAFYCEAFPGFFYFVGMKNETVGKFEPGHSPYYIVNEDVIPYGAALQASIATRYL 427

Query: 432 L---PKSTLIKGNSHDEL 446
           L   PKST  KG S DEL
Sbjct: 428 LEYSPKSTSTKGYSRDEL 445

BLAST of MS003890 vs. TAIR 10
Match: AT1G51760.1 (peptidase M20/M25/M40 family protein )

HSP 1 Score: 622.5 bits (1604), Expect = 2.7e-178
Identity = 305/440 (69.32%), Postives = 368/440 (83.64%), Query Frame = 0

Query: 9   RWSNLIFIFNALLPLLIPSSSSPSPEELSQIPLNFLQYAKNPEVFNWMVDIRRQIHENPE 68
           +W + + I + L P LI  SS+     LSQIP  FL  AK  + F+WMV IRR+IHENPE
Sbjct: 5   KWVSFVLILHLLNPTLISCSSN----GLSQIPSKFLTLAKRNDFFDWMVGIRRRIHENPE 64

Query: 69  LGFEEFETSKLIRAELDRLDISYKYPVAITGVVGFIGTGNPPFVAIRADMDALALQEGVE 128
           LG+EE ETSKL+RAEL+++ +SYKYPVA+TGVVG++GTG+ PFVA+RADMDALA+QE VE
Sbjct: 65  LGYEEVETSKLVRAELEKMGVSYKYPVAVTGVVGYVGTGHAPFVALRADMDALAMQEMVE 124

Query: 129 WEHKSKVPGKMHACGHDAHVAMLLGAAKILQEHRRLIQGTVVLVFQPAEEGGGGAKKMLE 188
           WEHKSKVPGKMHACGHDAH  MLLGAAK+L+EH   +QGTVVLVFQPAEEGGGGAKK++E
Sbjct: 125 WEHKSKVPGKMHACGHDAHTTMLLGAAKLLKEHEEELQGTVVLVFQPAEEGGGGAKKIVE 184

Query: 189 SGVLDNVEAIFGLHLTGKCPIGRVASKPGPILAASGFFEAVISGKGGHAALPQHTIDPIL 248
           +GVL+NV AIFGLH+T +  +G+V+S+ GP+LA SGFF+A ISGKGGHAALPQHTIDPIL
Sbjct: 185 AGVLENVSAIFGLHVTNQLALGQVSSREGPMLAGSGFFKAKISGKGGHAALPQHTIDPIL 244

Query: 249 AASNIIISLQHLISREADPLDSQVVTVAKFQGGGAFNVIPDSVTIGGTFRAFSDESFIQL 308
           AASN+I+SLQHL+SREADPLDSQVVTVAKF+GGGAFNVIPDSVTIGGTFRAFS +SF+QL
Sbjct: 245 AASNVIVSLQHLVSREADPLDSQVVTVAKFEGGGAFNVIPDSVTIGGTFRAFSTKSFMQL 304

Query: 309 KQRIVEVITRQANVQRCNATVQF-NEEKPFFPVTLNDPDLYKHFHSVAGDMLGTKNILDM 368
           K+RI +VITRQA+V  CNATV F  EEKPFFP T+ND  L++ F +V+GDMLG +N ++M
Sbjct: 305 KKRIEQVITRQASVNMCNATVDFIEEEKPFFPPTVNDKALHQFFKNVSGDMLGIENYVEM 364

Query: 369 QPIMGTEDFAFYGEVIPGFFFFVGMKNETLGMFESGHSPYYIVNEDVLPYGAALHASIAT 428
           QP+MG+EDF+FY + IPG F FVGM+N+      S HSPY+ VNE++LPYGA+LHAS+AT
Sbjct: 365 QPLMGSEDFSFYQQAIPGHFSFVGMQNKARSPMASPHSPYFEVNEELLPYGASLHASMAT 424

Query: 429 RYL--LPKSTLIKGNSHDEL 446
           RYL  L  STL K N  DEL
Sbjct: 425 RYLLELKASTLNKSNKKDEL 440

BLAST of MS003890 vs. TAIR 10
Match: AT5G56660.1 (IAA-leucine resistant (ILR)-like 2 )

HSP 1 Score: 549.3 bits (1414), Expect = 2.9e-156
Identity = 277/439 (63.10%), Postives = 341/439 (77.68%), Query Frame = 0

Query: 13  LIFIFNALLPLLIPSSSSP-SPEELSQIPLNFLQYAKNPEVFNWMVDIRRQIHENPELGF 72
           L   F  LL LL  SS SP   E+ SQI    L++AK+PEVF+WMV IRR+IHENPELG+
Sbjct: 7   LSLTFQLLLFLLSVSSESPWIAEDTSQIQTKLLEFAKSPEVFDWMVKIRRKIHENPELGY 66

Query: 73  EEFETSKLIRAELDRLDISYKYPVAITGVVGFIGTGNPPFVAIRADMDALALQEGVEWEH 132
           EE ETSKLIR+EL+ + I Y+YPVAITGV+G+IGTG PPFVA+RADMDAL +QEGVEWEH
Sbjct: 67  EELETSKLIRSELELIGIKYRYPVAITGVIGYIGTGEPPFVALRADMDALPIQEGVEWEH 126

Query: 133 KSKVPGKMHACGHDAHVAMLLGAAKILQEHRRLIQGTVVLVFQPAEEGGGGAKKMLESGV 192
           KSK+ GKMHACGHD HV MLLGAAKIL EHR  +QGTVVL+FQPAEEG  GAKKM E G 
Sbjct: 127 KSKIAGKMHACGHDGHVTMLLGAAKILHEHRHHLQGTVVLIFQPAEEGLSGAKKMREEGA 186

Query: 193 LDNVEAIFGLHLTGKCPIGRVASKPGPILAASGFFEAVISGKGGHAALPQHTIDPILAAS 252
           L NVEAIFG+HL+ + P G+ AS+ G  LA +G FEAVI+GKGGHAA+PQHTIDP++AAS
Sbjct: 187 LKNVEAIFGIHLSARIPFGKAASRAGSFLAGAGVFEAVITGKGGHAAIPQHTIDPVVAAS 246

Query: 253 NIIISLQHLISREADPLDSQVVTVAKFQGGGAFNVIPDSVTIGGTFRAFSDESFIQLKQR 312
           +I++SLQ L+SRE DPLDS+VVTV+K  GG AFNVIPDS+TIGGT RAF+   F QL+QR
Sbjct: 247 SIVLSLQQLVSRETDPLDSKVVTVSKVNGGNAFNVIPDSITIGGTLRAFT--GFTQLQQR 306

Query: 313 IVEVITRQANVQRCNATVQF--NEEKPFFPVTLNDPDLYKHFHSVAGDMLGTKNILDMQP 372
           + EVIT+QA V RCNA+V    N  +P  P T+N+ DLYK F  V  D+LG +  ++  P
Sbjct: 307 VKEVITKQAAVHRCNASVNLTPNGREP-MPPTVNNKDLYKQFKKVVRDLLGQEAFVEAAP 366

Query: 373 IMGTEDFAFYGEVIPGFFFFVGMKNETLGMFESGHSPYYIVNEDVLPYGAALHASIATRY 432
           +MG+EDF+++ E IPG F  +GM++ET G + S HSP Y +NEDVLPYGAA+HAS+A +Y
Sbjct: 367 VMGSEDFSYFAETIPGHFSLLGMQDETNG-YASSHSPLYRINEDVLPYGAAIHASMAVQY 426

Query: 433 LLPKSTLIKGN---SHDEL 446
           L  K++  KG+    H+EL
Sbjct: 427 LKEKAS--KGSVSGFHEEL 439

BLAST of MS003890 vs. TAIR 10
Match: AT1G51780.1 (IAA-leucine resistant (ILR)-like gene 5 )

HSP 1 Score: 545.8 bits (1405), Expect = 3.2e-155
Identity = 267/420 (63.57%), Postives = 337/420 (80.24%), Query Frame = 0

Query: 17  FNALLPLLIPSSSSPSPEELSQIPLNFLQYAKNPEVFNWMVDIRRQIHENPELGFEEFET 76
           F  +L LL     S S  +LSQIP NFL  AK  + F+WMV IRR+IHENPELG+EE ET
Sbjct: 9   FVLILHLLNSCLISCSSNDLSQIPKNFLSLAKREDFFDWMVGIRRRIHENPELGYEEVET 68

Query: 77  SKLIRAELDRLDISYKYPVAITGVVGFIGTGNPPFVAIRADMDALALQEGVEWEHKSKVP 136
           SKL++ ELD++ +SYK PVA+TGV+G++GTG+ PFVA+RADMDAL +QE VEWEHKSK+P
Sbjct: 69  SKLVKTELDKMGVSYKNPVAVTGVIGYVGTGHAPFVALRADMDALPIQEMVEWEHKSKIP 128

Query: 137 GKMHACGHDAHVAMLLGAAKILQEHRRLIQGTVVLVFQPAEEGGGGAKKMLESGVLDNVE 196
           GKMHACGHDAH  MLLGAAK+L+EH+  +QGTV+LVFQPAEEGG GAKK++E+GVL+NV 
Sbjct: 129 GKMHACGHDAHTTMLLGAAKLLKEHQEELQGTVILVFQPAEEGGAGAKKIVEAGVLENVG 188

Query: 197 AIFGLHLTGKCPIGRVASKPGPILAASGFFEAVISGKGGHAALPQHTIDPILAASNIIIS 256
           AIFGLH++    +G+++S+ G ++A SG F+A ISGKGGHAALPQ  IDP+LAASN+I+S
Sbjct: 189 AIFGLHVSNLLGLGQLSSREGLLMAGSGRFKATISGKGGHAALPQFAIDPVLAASNVILS 248

Query: 257 LQHLISREADPLDSQVVTVAKFQGGGAFNVIPDSVTIGGTFRAFSDESFIQLKQRIVEVI 316
           LQHL+SREADPLDSQVVTVA F+G  AFNVIPDSVTIGGTFRA   +SF QLKQRIV+VI
Sbjct: 249 LQHLVSREADPLDSQVVTVATFEGSDAFNVIPDSVTIGGTFRALLPKSFEQLKQRIVQVI 308

Query: 317 TRQANVQRCNATVQF-NEEKPFFPVTLNDPDLYKHFHSVAGDMLGTKNILDMQPIMGTED 376
           T QA+V  CNATV F  +E P FP T+N+  L+  + +V+ DMLG +N ++  P+M +ED
Sbjct: 309 TTQASVNMCNATVDFLEDETPPFPPTVNNKTLHLFYKNVSVDMLGIENYVETLPVMVSED 368

Query: 377 FAFYGEVIPGFFFFVGMKNETLGMFESGHSPYYIVNEDVLPYGAALHASIATRYLLPKST 436
           FAFY + IPG F FVGM+N++     + HSP++ VNE++LPYGA+L AS+ATRYLL  S+
Sbjct: 369 FAFYQQAIPGHFSFVGMQNKSHSPMANPHSPFFEVNEELLPYGASLLASLATRYLLDSSS 428

BLAST of MS003890 vs. TAIR 10
Match: AT5G56650.1 (IAA-leucine resistant (ILR)-like 1 )

HSP 1 Score: 540.4 bits (1391), Expect = 1.3e-153
Identity = 273/441 (61.90%), Postives = 342/441 (77.55%), Query Frame = 0

Query: 12  NLIFIFNALLPLLIPSSSSP--SPEELSQIPLNFLQYAKNPEVFNWMVDIRRQIHENPEL 71
           N    F  LL LL  SS SP     ++S+IP+NFL+ AK+PEVF+ MV IRR+IHENPEL
Sbjct: 4   NNFLTFQLLLLLLRVSSESPWIVAGDVSRIPINFLELAKSPEVFDSMVRIRRKIHENPEL 63

Query: 72  GFEEFETSKLIRAELDRLDISYKYPVAITGVVGFIGTGNPPFVAIRADMDALALQEGVEW 131
           G+EEFETSK IR+ELD + + Y++PVAITG++G+IGTG PPFVA+RADMDAL +QE VEW
Sbjct: 64  GYEEFETSKFIRSELDLIGVKYRFPVAITGIIGYIGTGEPPFVALRADMDALPIQEAVEW 123

Query: 132 EHKSKVPGKMHACGHDAHVAMLLGAAKILQEHRRLIQGTVVLVFQPAEEGGGGAKKMLES 191
           EHKSK PGKMHACGHD HVAMLLGAAKILQ+HR+ +QGTVVL+FQPAEEG  GAK M E 
Sbjct: 124 EHKSKNPGKMHACGHDGHVAMLLGAAKILQQHRQHLQGTVVLIFQPAEEGLSGAKMMREE 183

Query: 192 GVLDNVEAIFGLHLTGKCPIGRVASKPGPILAASGFFEAVISGKGGHAALPQHTIDPILA 251
           G L NVEAIFG+HL+ + P G+ AS  G  +A +G FEAVI+GKGGHAA+PQHTIDP++A
Sbjct: 184 GALKNVEAIFGIHLSPRTPFGKAASLAGSFMAGAGAFEAVITGKGGHAAIPQHTIDPVVA 243

Query: 252 ASNIIISLQHLISREADPLDSQVVTVAKFQGGGAFNVIPDSVTIGGTFRAFSDESFIQLK 311
           AS+I++SLQHL+SRE DP DS+VVTV K  GG AFNVIPDS+TIGGT RAF+   F QL+
Sbjct: 244 ASSIVLSLQHLVSRETDPSDSKVVTVTKVNGGNAFNVIPDSITIGGTLRAFT--GFTQLQ 303

Query: 312 QRIVEVITRQANVQRCNATVQF--NEEKPFFPVTLNDPDLYKHFHSVAGDMLGTKNILDM 371
           +RI E+IT+QA V RCNA+V    N  +P  P T+N+ DLYK F  V  D+LG +  ++ 
Sbjct: 304 ERIKEIITKQAAVHRCNASVNLAPNGNQP-MPPTVNNMDLYKKFKKVVRDLLGQEAFVEA 363

Query: 372 QPIMGTEDFAFYGEVIPGFFFFVGMKNETLGMFESGHSPYYIVNEDVLPYGAALHASIAT 431
            P MG+EDF+++ E IPG F  +GM++ET G + S HSP+Y +NEDVLPYGAA+HA++A 
Sbjct: 364 VPEMGSEDFSYFAETIPGHFSLLGMQDETQG-YASSHSPHYRINEDVLPYGAAIHATMAV 423

Query: 432 RYLLPKSTLIKGN---SHDEL 446
           +YL  K++  KG+    HDEL
Sbjct: 424 QYLKDKAS--KGSVSGFHDEL 438

BLAST of MS003890 vs. TAIR 10
Match: AT1G44350.1 (IAA-leucine resistant (ILR)-like gene 6 )

HSP 1 Score: 429.1 bits (1102), Expect = 4.3e-120
Identity = 212/391 (54.22%), Postives = 274/391 (70.08%), Query Frame = 0

Query: 44  LQYAKNPEVFNWMVDIRRQIHENPELGFEEFETSKLIRAELDRLDISYKYPVAITGVVGF 103
           L+    P+   W+  +RR IHENPEL FEE+ETS+LIR+ELDR+ I Y+YP+A TG+  +
Sbjct: 77  LRLTYQPDNVAWLKRVRRTIHENPELAFEEYETSRLIRSELDRMGIMYRYPLAKTGIRAW 136

Query: 104 IGTGNPPFVAIRADMDALALQEGVEWEHKSKVPGKMHACGHDAHVAMLLGAAKILQEHRR 163
           IG+G PPFVA+RADMDAL +QE VEWEH SKV GKMHACGHDAHV MLLGAA IL+    
Sbjct: 137 IGSGGPPFVAVRADMDALPIQEAVEWEHISKVAGKMHACGHDAHVTMLLGAAHILKAREH 196

Query: 164 LIQGTVVLVFQPAEEGGGGAKKMLESGVLDNVEAIFGLHLTGKCPIGRVASKPGPILAAS 223
           L++GTVVL+FQPAEE G GAK M+E G LD+VEAIF +H++   P G + S+ GP+LA  
Sbjct: 197 LLKGTVVLLFQPAEEAGNGAKNMIEDGALDDVEAIFAVHVSHIHPTGVIGSRSGPLLAGC 256

Query: 224 GFFEAVISGKGGHAALPQHTIDPILAASNIIISLQHLISREADPLDSQVVTVAKFQGGGA 283
           G F AVI+ +    A      + +LAAS+ +ISLQ ++SREA PLDSQVV+V  F GG +
Sbjct: 257 GIFRAVITSEDSRGA-----ANLLLAASSAVISLQGIVSREASPLDSQVVSVTSFDGGHS 316

Query: 284 FNVIPDSVTIGGTFRAFSDESFIQLKQRIVEVITRQANVQRCNATVQFNE-EKPFFPVTL 343
            +V PD+V +GGTFRAFS+ SF  LK+RI EV+  Q  V  C ATV F E +   +P T 
Sbjct: 317 LDVAPDTVVLGGTFRAFSNSSFYYLKKRIQEVLMDQVGVFGCQATVNFFEKQNAIYPPTT 376

Query: 344 NDPDLYKHFHSVAGDMLGTKNILDMQPIMGTEDFAFYGEVIPGFFFFVGMKNETLGMFES 403
           N+   Y H   V  D+LG  +      +MG EDFAFY E+IP  F+F+G++NE LG    
Sbjct: 377 NNDATYNHLKKVTIDLLGDSHFTLAPQMMGAEDFAFYSEIIPAAFYFIGIRNEELGSVHI 436

Query: 404 GHSPYYIVNEDVLPYGAALHASIATRYLLPK 434
            HSP+++++ED LP GAA+HA++A RYL  K
Sbjct: 437 AHSPHFMIDEDSLPVGAAVHAAVAERYLNDK 462

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022145035.12.5e-255100.00IAA-amino acid hydrolase ILR1-like 4 [Momordica charantia][more]
XP_038878754.12.0e-22086.14IAA-amino acid hydrolase ILR1-like 4 [Benincasa hispida][more]
XP_008450808.18.3e-21485.16PREDICTED: IAA-amino acid hydrolase ILR1-like 4 [Cucumis melo] >TYK10129.1 IAA-a... [more]
KAG6588014.15.9e-21285.16IAA-amino acid hydrolase ILR1-like 4, partial [Cucurbita argyrosperma subsp. sor... [more]
XP_004135685.17.7e-21283.60IAA-amino acid hydrolase ILR1-like 4 [Cucumis sativus] >KGN66199.1 hypothetical ... [more]
Match NameE-valueIdentityDescription
O043733.8e-17769.32IAA-amino acid hydrolase ILR1-like 4 OS=Arabidopsis thaliana OX=3702 GN=ILL4 PE=... [more]
Q84XG92.4e-16368.54IAA-amino acid hydrolase ILR1-like 1 OS=Oryza sativa subsp. indica OX=39946 GN=I... [more]
Q8S9S49.0e-16368.29IAA-amino acid hydrolase ILR1-like 1 OS=Oryza sativa subsp. japonica OX=39947 GN... [more]
P549704.1e-15563.10IAA-amino acid hydrolase ILR1-like 2 OS=Arabidopsis thaliana OX=3702 GN=ILL2 PE=... [more]
Q9SWX94.5e-15463.57IAA-amino acid hydrolase ILR1-like 5 OS=Arabidopsis thaliana OX=3702 GN=ILL5 PE=... [more]
Match NameE-valueIdentityDescription
A0A6J1CTB61.2e-255100.00IAA-amino acid hydrolase ILR1-like 4 OS=Momordica charantia OX=3673 GN=LOC111014... [more]
A0A5D3CIQ74.0e-21485.16IAA-amino acid hydrolase ILR1-like 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E... [more]
A0A1S3BQ364.0e-21485.16IAA-amino acid hydrolase ILR1-like 4 OS=Cucumis melo OX=3656 GN=LOC103492283 PE=... [more]
A0A0A0LYS13.7e-21283.60M20_dimer domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G575150 P... [more]
A0A6J1F5X76.0e-21084.47IAA-amino acid hydrolase ILR1-like 4 isoform X1 OS=Cucurbita moschata OX=3662 GN... [more]
Match NameE-valueIdentityDescription
AT1G51760.12.7e-17869.32peptidase M20/M25/M40 family protein [more]
AT5G56660.12.9e-15663.10IAA-leucine resistant (ILR)-like 2 [more]
AT1G51780.13.2e-15563.57IAA-leucine resistant (ILR)-like gene 5 [more]
AT5G56650.11.3e-15361.90IAA-leucine resistant (ILR)-like 1 [more]
AT1G44350.14.3e-12054.22IAA-leucine resistant (ILR)-like gene 6 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR017439AmidohydrolasePIRSFPIRSF005962Amidohydrol_AmhXcoord: 42..434
e-value: 1.5E-127
score: 423.7
IPR017439AmidohydrolaseTIGRFAMTIGR01891TIGR01891coord: 56..417
e-value: 7.5E-114
score: 378.6
IPR017439AmidohydrolasePANTHERPTHR11014PEPTIDASE M20 FAMILY MEMBERcoord: 6..445
NoneNo IPR availableGENE3D3.40.630.10Zn peptidasescoord: 56..415
e-value: 2.5E-140
score: 469.4
NoneNo IPR availableGENE3D3.30.70.360coord: 221..338
e-value: 2.5E-140
score: 469.4
NoneNo IPR availablePANTHERPTHR11014:SF119IAA-AMINO ACID HYDROLASE ILR1-LIKE 4coord: 6..445
NoneNo IPR availableSUPERFAMILY53187Zn-dependent exopeptidasescoord: 51..431
IPR002933Peptidase M20PFAMPF01546Peptidase_M20coord: 113..426
e-value: 2.2E-34
score: 119.2
IPR011650Peptidase M20, dimerisation domainPFAMPF07687M20_dimercoord: 226..320
e-value: 4.1E-10
score: 39.5
IPR044757IAA-amino acid hydrolase ILR1-likeCDDcd08017M20_IAA_Hydcoord: 56..430
e-value: 0.0
score: 650.536
IPR036264Bacterial exopeptidase dimerisation domainSUPERFAMILY55031Bacterial exopeptidase dimerisation domaincoord: 221..336

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS003890.1MS003890.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009850 auxin metabolic process
molecular_function GO:0016787 hydrolase activity