MS003866 (gene) Bitter gourd (TR) v1

Overview


Name	MS003866
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	SpoU_sub_bind domain-containing protein
Location	scaffold127: 1109240 .. 1110817 (-)
RNA-Seq Expression	MS003866
Synteny	MS003866

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAGCTTTCAGTTCGCTCTCGCCACTGCTTTCCAGAAATTAGGGAATTAGTAAAACCTGAATTGAGTTCTACGAGATGTTTGTCATTGGGGAAATTTCGAAGTTTTTCGAGTTCCCACCCTCTTAAAGATGAAGGAGTGAGATCTGGGAATAAACTTCCTTGGTTAGCAAAGTCAAAATGGTCGGAGAAATCATCAGGAAAGACGGGAAGAGACCAATCGTCTTGGAAGGATTCAGATACGAGGTCAGGAAAGATGAGATCTTCATGGGGCAATCCAGCAAAGACATTTGGGAAGAAAAGTAATAGGTCATCTGGGGATAAATCAGAAAAGAGATATGGCCAATCGCCAACTAGAACTGCTACCCGTTCTACTTGGGAGCTCTCTGCCGAGAATTATGCTGGACGAGACATTGATTCTACTGATGAAATAATACAGGGTAGGAGAATAAGAGTGGACAACGAGGAGGAGGAACGAAATGATGATAACGAAAGACGGGGAAGTTACGAAGAAGTAGAAGATGAAGAAGCAGAGGCCATTGCTGATCCCAGGTGGGATAAGATTAAAAGTAGATTTAGGGGAACGGTGGACGAAAATGCTGCACCCGAGAGACCCGAGTTTAAGAGGTGGAATAAACAGGAAAGCTGGGGTAGAAAAACATGGAGGGAAGCTTCGGAATCCACCATCCCTAAAATGGTTGGTGAAGGGGTTTATGGGGTTGGACCAGTTTTGGCTGCTTTGTCAGCTGGAAGAAGAGAGTTCTATGCATTGTATATGCAAGAAGGACTGGACTTGAACAACAACAATAAGAAGAAGAAGGACAAGAAAGGCTTTGAGAAAGTTCTGAGGTTGGCTGAAAGGAATGCATTATCTATAAAGGAAATATCGAAGCATGATCTCAATATGATTTGCGATAACCGCCCGCATCAAGGTCTTGTGCTGGATGCTTCTCCTCTGGAGATGGTAAAAATAAAAGAATTGGACCCTGTTTCGCTCGAAGAAGACAAAGGCTCACTTTGGTTAGCTTTGGACGAGGTTACAGACCCCCAGAACTTGGGGGCAATCATAAGATCAGCTTATTTCTTTGGTGCATCAGGGGTTGTGCTATGTGCAAAGAATTCAGCTCCATTAAGTGGTGTTGTTAGTAAAGCAAGTGCCGGTTCACTTGAGCTAATGGAGCTTAGATATTGTACGAACATGATGCAATTCTTAACATCCTCAGCCGAAAACGGTTGGAGAGTCCTCGGAGCTTCAGTTTCTTCAAAATCCCTTGATTTGAAAGAGGTACAACCCGGTGCACCGACCATTCTAGTTCTAGGCAGTGAAGGCACTGGTTTGAGGCCCTTGGTAGAGAGATCCTGCTCACAGCTCGTTCGGATCCCTGGGAATATTCCAGTTGATGTAACCATGGAGAAAGACAACGATGCCGAACCCGCACAACCAACCAATGGGTGCTCGATAGACGAGTTTCGATCGTTTATGGCCGTGGAGAGCTTAAACGTCAGTGTAGCAGCAGGTGTGCTTGTTCACCACTTAATTGGAAGCAATTACATAAACGATCCATTAGAAACCTGTGTG

mRNA sequence

ATGAAGCTTTCAGTTCGCTCTCGCCACTGCTTTCCAGAAATTAGGGAATTAGTAAAACCTGAATTGAGTTCTACGAGATGTTTGTCATTGGGGAAATTTCGAAGTTTTTCGAGTTCCCACCCTCTTAAAGATGAAGGAGTGAGATCTGGGAATAAACTTCCTTGGTTAGCAAAGTCAAAATGGTCGGAGAAATCATCAGGAAAGACGGGAAGAGACCAATCGTCTTGGAAGGATTCAGATACGAGGTCAGGAAAGATGAGATCTTCATGGGGCAATCCAGCAAAGACATTTGGGAAGAAAAGTAATAGGTCATCTGGGGATAAATCAGAAAAGAGATATGGCCAATCGCCAACTAGAACTGCTACCCGTTCTACTTGGGAGCTCTCTGCCGAGAATTATGCTGGACGAGACATTGATTCTACTGATGAAATAATACAGGGTAGGAGAATAAGAGTGGACAACGAGGAGGAGGAACGAAATGATGATAACGAAAGACGGGGAAGTTACGAAGAAGTAGAAGATGAAGAAGCAGAGGCCATTGCTGATCCCAGGTGGGATAAGATTAAAAGTAGATTTAGGGGAACGGTGGACGAAAATGCTGCACCCGAGAGACCCGAGTTTAAGAGGTGGAATAAACAGGAAAGCTGGGGTAGAAAAACATGGAGGGAAGCTTCGGAATCCACCATCCCTAAAATGGTTGGTGAAGGGGTTTATGGGGTTGGACCAGTTTTGGCTGCTTTGTCAGCTGGAAGAAGAGAGTTCTATGCATTGTATATGCAAGAAGGACTGGACTTGAACAACAACAATAAGAAGAAGAAGGACAAGAAAGGCTTTGAGAAAGTTCTGAGGTTGGCTGAAAGGAATGCATTATCTATAAAGGAAATATCGAAGCATGATCTCAATATGATTTGCGATAACCGCCCGCATCAAGGTCTTGTGCTGGATGCTTCTCCTCTGGAGATGGTAAAAATAAAAGAATTGGACCCTGTTTCGCTCGAAGAAGACAAAGGCTCACTTTGGTTAGCTTTGGACGAGGTTACAGACCCCCAGAACTTGGGGGCAATCATAAGATCAGCTTATTTCTTTGGTGCATCAGGGGTTGTGCTATGTGCAAAGAATTCAGCTCCATTAAGTGGTGTTGTTAGTAAAGCAAGTGCCGGTTCACTTGAGCTAATGGAGCTTAGATATTGTACGAACATGATGCAATTCTTAACATCCTCAGCCGAAAACGGTTGGAGAGTCCTCGGAGCTTCAGTTTCTTCAAAATCCCTTGATTTGAAAGAGGTACAACCCGGTGCACCGACCATTCTAGTTCTAGGCAGTGAAGGCACTGGTTTGAGGCCCTTGGTAGAGAGATCCTGCTCACAGCTCGTTCGGATCCCTGGGAATATTCCAGTTGATGTAACCATGGAGAAAGACAACGATGCCGAACCCGCACAACCAACCAATGGGTGCTCGATAGACGAGTTTCGATCGTTTATGGCCGTGGAGAGCTTAAACGTCAGTGTAGCAGCAGGTGTGCTTGTTCACCACTTAATTGGAAGCAATTACATAAACGATCCATTAGAAACCTGTGTG

Coding sequence (CDS)

ATGAAGCTTTCAGTTCGCTCTCGCCACTGCTTTCCAGAAATTAGGGAATTAGTAAAACCTGAATTGAGTTCTACGAGATGTTTGTCATTGGGGAAATTTCGAAGTTTTTCGAGTTCCCACCCTCTTAAAGATGAAGGAGTGAGATCTGGGAATAAACTTCCTTGGTTAGCAAAGTCAAAATGGTCGGAGAAATCATCAGGAAAGACGGGAAGAGACCAATCGTCTTGGAAGGATTCAGATACGAGGTCAGGAAAGATGAGATCTTCATGGGGCAATCCAGCAAAGACATTTGGGAAGAAAAGTAATAGGTCATCTGGGGATAAATCAGAAAAGAGATATGGCCAATCGCCAACTAGAACTGCTACCCGTTCTACTTGGGAGCTCTCTGCCGAGAATTATGCTGGACGAGACATTGATTCTACTGATGAAATAATACAGGGTAGGAGAATAAGAGTGGACAACGAGGAGGAGGAACGAAATGATGATAACGAAAGACGGGGAAGTTACGAAGAAGTAGAAGATGAAGAAGCAGAGGCCATTGCTGATCCCAGGTGGGATAAGATTAAAAGTAGATTTAGGGGAACGGTGGACGAAAATGCTGCACCCGAGAGACCCGAGTTTAAGAGGTGGAATAAACAGGAAAGCTGGGGTAGAAAAACATGGAGGGAAGCTTCGGAATCCACCATCCCTAAAATGGTTGGTGAAGGGGTTTATGGGGTTGGACCAGTTTTGGCTGCTTTGTCAGCTGGAAGAAGAGAGTTCTATGCATTGTATATGCAAGAAGGACTGGACTTGAACAACAACAATAAGAAGAAGAAGGACAAGAAAGGCTTTGAGAAAGTTCTGAGGTTGGCTGAAAGGAATGCATTATCTATAAAGGAAATATCGAAGCATGATCTCAATATGATTTGCGATAACCGCCCGCATCAAGGTCTTGTGCTGGATGCTTCTCCTCTGGAGATGGTAAAAATAAAAGAATTGGACCCTGTTTCGCTCGAAGAAGACAAAGGCTCACTTTGGTTAGCTTTGGACGAGGTTACAGACCCCCAGAACTTGGGGGCAATCATAAGATCAGCTTATTTCTTTGGTGCATCAGGGGTTGTGCTATGTGCAAAGAATTCAGCTCCATTAAGTGGTGTTGTTAGTAAAGCAAGTGCCGGTTCACTTGAGCTAATGGAGCTTAGATATTGTACGAACATGATGCAATTCTTAACATCCTCAGCCGAAAACGGTTGGAGAGTCCTCGGAGCTTCAGTTTCTTCAAAATCCCTTGATTTGAAAGAGGTACAACCCGGTGCACCGACCATTCTAGTTCTAGGCAGTGAAGGCACTGGTTTGAGGCCCTTGGTAGAGAGATCCTGCTCACAGCTCGTTCGGATCCCTGGGAATATTCCAGTTGATGTAACCATGGAGAAAGACAACGATGCCGAACCCGCACAACCAACCAATGGGTGCTCGATAGACGAGTTTCGATCGTTTATGGCCGTGGAGAGCTTAAACGTCAGTGTAGCAGCAGGTGTGCTTGTTCACCACTTAATTGGAAGCAATTACATAAACGATCCATTAGAAACCTGTGTG

Protein sequence

MKLSVRSRHCFPEIRELVKPELSSTRCLSLGKFRSFSSSHPLKDEGVRSGNKLPWLAKSKWSEKSSGKTGRDQSSWKDSDTRSGKMRSSWGNPAKTFGKKSNRSSGDKSEKRYGQSPTRTATRSTWELSAENYAGRDIDSTDEIIQGRRIRVDNEEEERNDDNERRGSYEEVEDEEAEAIADPRWDKIKSRFRGTVDENAAPERPEFKRWNKQESWGRKTWREASESTIPKMVGEGVYGVGPVLAALSAGRREFYALYMQEGLDLNNNNKKKKDKKGFEKVLRLAERNALSIKEISKHDLNMICDNRPHQGLVLDASPLEMVKIKELDPVSLEEDKGSLWLALDEVTDPQNLGAIIRSAYFFGASGVVLCAKNSAPLSGVVSKASAGSLELMELRYCTNMMQFLTSSAENGWRVLGASVSSKSLDLKEVQPGAPTILVLGSEGTGLRPLVERSCSQLVRIPGNIPVDVTMEKDNDAEPAQPTNGCSIDEFRSFMAVESLNVSVAAGVLVHHLIGSNYINDPLETCV

Homology

[Close]

BLAST of MS003866 vs. NCBI nr
Match: XP_022145044.1 (uncharacterized protein LOC111014564 [Momordica charantia] >XP_022145045.1 uncharacterized protein LOC111014564 [Momordica charantia] >XP_022145047.1 uncharacterized protein LOC111014564 [Momordica charantia])

HSP 1 Score: 982.2 bits (2538), Expect = 1.7e-282
Identity = 507/513 (98.83%), Postives = 508/513 (99.03%), Query Frame = 0


Query: 1   MKLSVRSRHCFPEIRELVKPELSSTRCLSLGKFRSFSSSHPLKDEGVRSGNKLPWLAKSK 60
           MKLSVRSRHCFPEIRELVKPELSSTRCLSLGKFRSFSSSHPLKDEGVRSGNKLPWLAKSK
Sbjct: 59  MKLSVRSRHCFPEIRELVKPELSSTRCLSLGKFRSFSSSHPLKDEGVRSGNKLPWLAKSK 118

Query: 61  WSEKSSGKTGRDQSSWKDSDTRSGKMRSSWGNPAKTFGKKSNRSSGDKSEKRYGQSPTRT 120
           WSEKSSGKTGRDQSSWKDSDTRSGKMRSSWGNPAKTFGKKSNRSSGDKSEKRYGQSPTRT
Sbjct: 119 WSEKSSGKTGRDQSSWKDSDTRSGKMRSSWGNPAKTFGKKSNRSSGDKSEKRYGQSPTRT 178

Query: 121 ATRSTWELSAENYAGRDIDSTDEIIQGRRIRVDNEEEERNDDNERRGSYEEVEDEEAEAI 180
           ATRSTWELSAENYAGRDIDSTDEIIQGRRIRVDN EEERNDDNERRGSYEEVEDEEAEAI
Sbjct: 179 ATRSTWELSAENYAGRDIDSTDEIIQGRRIRVDN-EEERNDDNERRGSYEEVEDEEAEAI 238

Query: 181 ADPRWDKIKSRFRGTVDENAAPERPEFKRWNKQESWGRKTWREASESTIPKMVGEGVYGV 240
           ADPRWDKIKSRFRGTVDENAAPERPEFKRWNKQESWGRKTWREASESTIPKMVGEGVYGV
Sbjct: 239 ADPRWDKIKSRFRGTVDENAAPERPEFKRWNKQESWGRKTWREASESTIPKMVGEGVYGV 298

Query: 241 GPVLAALSAGRREFYALYMQEGLDLNNNNKKKKDKKGFEKVLRLAERNALSIKEISKHDL 300
           GPVLAALSAGRREFYALYMQEGLDL NNNK KKDKKGFEKVLRLAERNALSIKEISKHDL
Sbjct: 299 GPVLAALSAGRREFYALYMQEGLDLKNNNKTKKDKKGFEKVLRLAERNALSIKEISKHDL 358

Query: 301 NMICDNRPHQGLVLDASPLEMVKIKELDPVSLEEDKGSLWLALDEVTDPQNLGAIIRSAY 360
           NMICDNRPHQGLVLDASPLEMVKIKELDPVSLEEDKGSLWLALDEVTDPQNLGAIIRSAY
Sbjct: 359 NMICDNRPHQGLVLDASPLEMVKIKELDPVSLEEDKGSLWLALDEVTDPQNLGAIIRSAY 418

Query: 361 FFGASGVVLCAKNSAPLSGVVSKASAGSLELMELRYCTNMMQFLTSSAENGWRVLGASVS 420
           FFGASGVVLCAKNSAPLSGVVSKASAGSLELMELRYC NMMQFLTSSAENGWRVLGASVS
Sbjct: 419 FFGASGVVLCAKNSAPLSGVVSKASAGSLELMELRYCKNMMQFLTSSAENGWRVLGASVS 478

Query: 421 SKSLDLKEVQPGAPTILVLGSEGTGLRPLVERSCSQLVRIPGNIPVDVTMEKDNDAEPAQ 480
           SKSLDLKEVQPGAPTILVLGSEGTGLRPLVERSCSQLVR+PGNIPVDVTMEKDNDAEPAQ
Sbjct: 479 SKSLDLKEVQPGAPTILVLGSEGTGLRPLVERSCSQLVRVPGNIPVDVTMEKDNDAEPAQ 538

Query: 481 PTNGCSIDEFRSFMAVESLNVSVAAGVLVHHLI 514
            TNGCSIDEFRSFMAVESLNVSVAAGVLVHHLI
Sbjct: 539 TTNGCSIDEFRSFMAVESLNVSVAAGVLVHHLI 570

[Close]

BLAST of MS003866 vs. NCBI nr
Match: XP_038880007.1 (uncharacterized protein LOC120071698 [Benincasa hispida] >XP_038880008.1 uncharacterized protein LOC120071698 [Benincasa hispida])

HSP 1 Score: 798.5 bits (2061), Expect = 3.5e-227
Identity = 421/537 (78.40%), Postives = 464/537 (86.41%), Query Frame = 0


Query: 1   MKLSVRSRHCFPEIRELVKPEL--------SSTRCLSLGKFRSFSSSHPLKDEGVRSGNK 60
           MKLS+R RHCFPEIR+LV P+L        SS R  S  K R F SS+PLKDEGV SGNK
Sbjct: 61  MKLSIRGRHCFPEIRDLVSPQLNSSALSIGSSLRSFSSKKSRRFLSSYPLKDEGVLSGNK 120

Query: 61  LPWLA------KSKWSEKSSGKTGRD---QSSWKDSDTRSGKMRSSWGNPAKTFGKKSNR 120
           LPWLA      K+KWS+KS+ + G D   +SSWK+SD+RS +MRSSW    KT G+K NR
Sbjct: 121 LPWLAAANSLEKAKWSDKSTKRKGGDRSSRSSWKESDSRSDRMRSSWDYSTKTSGEKGNR 180

Query: 121 SSGDKSEKRYGQSPTRTATRSTWELSAENYAGRDIDSTDEIIQGRRIRVDNEEEERNDDN 180
           SS D+ EKR G++P  +A RSTWE+SAE +A + I S DE  + RR  VDNEE++ NDD 
Sbjct: 181 SSRDEPEKRNGKAPIGSANRSTWEVSAEKFARQGIKSADERRESRRTIVDNEEQQ-NDDI 240

Query: 181 ERRGSYEEVEDEEAEAIADPRWDKIKSRFRGTVDENAAPERPEFKRWNKQESWGRKTWRE 240
           ER GSYEEVEDEE EAIADPRWDKIKSRF+G VDE AAPERPEF+RWNKQESWGRKTWRE
Sbjct: 241 ERGGSYEEVEDEETEAIADPRWDKIKSRFKGMVDERAAPERPEFQRWNKQESWGRKTWRE 300

Query: 241 ASESTIPKMVGEGVYGVGPVLAALSAGRREFYALYMQEGLDLNNNNKKKKDKKGFEKVLR 300
           ASEST+PKM+GEGVYG+GPVLAALSAGRREFYALYMQEG+DLNNNNKKKKDKKGFEKVLR
Sbjct: 301 ASESTLPKMIGEGVYGIGPVLAALSAGRREFYALYMQEGIDLNNNNKKKKDKKGFEKVLR 360

Query: 301 LAERNALSIKEISKHDLNMICDNRPHQGLVLDASPLEMVKIKELDPVSLEEDKGSLWLAL 360
           LAE+NAL+IKEISKHDLNMI DNRPHQGLVLDASPLEMVKIKELDPV LEEDK SLWLAL
Sbjct: 361 LAEKNALTIKEISKHDLNMISDNRPHQGLVLDASPLEMVKIKELDPVLLEEDKDSLWLAL 420

Query: 361 DEVTDPQNLGAIIRSAYFFGASGVVLCAKNSAPLSGVVSKASAGSLELMELRYCTNMMQF 420
           DEVTDPQNLGAIIRSAYFFGASGVVLCAKNSAPLSGVVSKASAGSLELMELRYC NMMQF
Sbjct: 421 DEVTDPQNLGAIIRSAYFFGASGVVLCAKNSAPLSGVVSKASAGSLELMELRYCKNMMQF 480

Query: 421 LTSSAENGWRVLGASVSSKSLDLKEVQPGAPTILVLGSEGTGLRPLVERSCSQLVRIPGN 480
           LTSSAENGWRVLGASVSSKS+ L E+ PGAPTILVLGSEGTGLRPLVERSCSQLVRIPGN
Sbjct: 481 LTSSAENGWRVLGASVSSKSIALNEIAPGAPTILVLGSEGTGLRPLVERSCSQLVRIPGN 540

Query: 481 IPVDVTMEKDNDAEPAQPTNGCSIDEFRSFMAVESLNVSVAAGVLVHHLIGSNYIND 521
           IPV+VT++K+ND E  QP+N C +DEF+SFMAVESLNVSVAAGVL+HHLIG+NY N+
Sbjct: 541 IPVEVTIDKENDIETTQPSNVCLVDEFQSFMAVESLNVSVAAGVLIHHLIGNNYKNE 596

[Close]

BLAST of MS003866 vs. NCBI nr
Match: XP_004135672.1 (uncharacterized protein LOC101220884 [Cucumis sativus] >XP_031739747.1 uncharacterized protein LOC101220884 [Cucumis sativus] >KGN66175.1 hypothetical protein Csa_007609 [Cucumis sativus])

HSP 1 Score: 783.1 bits (2021), Expect = 1.5e-222
Identity = 416/540 (77.04%), Postives = 460/540 (85.19%), Query Frame = 0


Query: 1   MKLSVRSRHCFPEIRELVKPEL--------SSTRCLSLGKFRSFSSSHPLKDEGVRSGNK 60
           +KLS+R RHCFPEIR+LV P+L        SS R +SL K R FSSS+ LKDEGV SGNK
Sbjct: 5   VKLSIRGRHCFPEIRDLVSPQLNSLPLSVGSSLRSVSLNKSRCFSSSYRLKDEGVLSGNK 64

Query: 61  LPWLA------KSKWSEKSSGKTGRDQSSWKDSDTRSGKMRSSWGNPAKTFGKKSNRSSG 120
           LPWLA      K+KW +KS+ K   D+S WK+SD +S           K FGK  NRSS 
Sbjct: 65  LPWLAAASSVEKAKWLDKSTTKKRGDRSPWKESDFKS-----------KRFGKNGNRSSR 124

Query: 121 DKSEKRYGQSPTRTATRSTWELSAENYAGRDIDSTDEIIQGRRIRVDNEEEERNDDNERR 180
           D+ EKRYG+ P  +A RSTWE+SAE +A R I+  DE  + RR R+DN EE+++DD ERR
Sbjct: 125 DEPEKRYGKEPIGSANRSTWEVSAEKFARRSIELADEKRESRRTRMDN-EEQKDDDIERR 184

Query: 181 GSYEEVEDEEAEAIADPRWDKIKSRFRGTVDENAAPERPEFKRWNKQESWGRKTWREASE 240
           GSYEEVEDEE EAIADPRWDKIKSRFRG VDE  A ERPEF+RWNKQESWGRKTW+EASE
Sbjct: 185 GSYEEVEDEETEAIADPRWDKIKSRFRGMVDERGATERPEFQRWNKQESWGRKTWKEASE 244

Query: 241 STIPKMVGEGVYGVGPVLAALSAGRREFYALYMQEGLDLNNNNKKKKDKKGFEKVLRLAE 300
           ST+PKM+GEGVYGVGPVLAALSAGRREFYALY+QEG+DLNNNNKKKKDKKGFEKVL+LAE
Sbjct: 245 STLPKMIGEGVYGVGPVLAALSAGRREFYALYVQEGIDLNNNNKKKKDKKGFEKVLKLAE 304

Query: 301 RNALSIKEISKHDLNMICDNRPHQGLVLDASPLEMVKIKELDPVSLEEDKGSLWLALDEV 360
           +NAL+IKEISKHDLNMI DNRPHQGLVLDASPLEMVKIKELDPVSLE+DKGSLWLALDEV
Sbjct: 305 KNALTIKEISKHDLNMISDNRPHQGLVLDASPLEMVKIKELDPVSLEDDKGSLWLALDEV 364

Query: 361 TDPQNLGAIIRSAYFFGASGVVLCAKNSAPLSGVVSKASAGSLELMELRYCTNMMQFLTS 420
           TDPQNLGAIIRSAYFFGASGVVLCAKNSAPLSGVVSKASAGSLELMELRYC NMMQFLTS
Sbjct: 365 TDPQNLGAIIRSAYFFGASGVVLCAKNSAPLSGVVSKASAGSLELMELRYCKNMMQFLTS 424

Query: 421 SAENGWRVLGASVSSKSLDLKEVQPGAPTILVLGSEGTGLRPLVERSCSQLVRIPGNIPV 480
           SAENGWRVLGASVSSKS+DLKEV PGAPTILVLGSEGTGLRPLVERSCSQLVRIPGNIPV
Sbjct: 425 SAENGWRVLGASVSSKSIDLKEVLPGAPTILVLGSEGTGLRPLVERSCSQLVRIPGNIPV 484

Query: 481 DVTMEKDNDAEPAQPTNGCSIDEFRSFMAVESLNVSVAAGVLVHHLIGSNYINDPLETCV 527
           DVT++ +ND E    T+G S+DEFRSFMAVESLNVSVAAGV VHHL+G++Y N+ LET +
Sbjct: 485 DVTIDTENDIE---TTDGRSVDEFRSFMAVESLNVSVAAGVFVHHLVGNSYKNESLETSI 529

[Close]

BLAST of MS003866 vs. NCBI nr
Match: XP_008450781.1 (PREDICTED: uncharacterized tRNA/rRNA methyltransferase MSMEG_6073/MSMEI_5913 [Cucumis melo] >TYK10149.1 putative tRNA/rRNA methyltransferase [Cucumis melo var. makuwa])

HSP 1 Score: 778.1 bits (2008), Expect = 4.8e-221
Identity = 416/538 (77.32%), Postives = 459/538 (85.32%), Query Frame = 0


Query: 1   MKLSVRSRHCFPEIRELVKPEL--------SSTRCLSLGKFRSFSSSHPLKDEGVRSGNK 60
           +KLS+R RHCFPEIR+LV P+L        SS + LSL K R FSSS+ LKDEGV SGNK
Sbjct: 5   VKLSIRGRHCFPEIRDLVSPQLNSLPLSVGSSLKSLSLNKSRCFSSSYRLKDEGVLSGNK 64

Query: 61  LPWLA------KSKWSEKSSGKTGRDQSSWKDSDTRSGKMRSSWGNPAKTFGKKSNRSSG 120
           LPWLA      K+KW +KS+ K   D+SSWK+SD +S           K FGK  NRSS 
Sbjct: 65  LPWLAAANSVEKAKWLDKSTTKKRGDRSSWKESDFKS-----------KRFGKNGNRSSR 124

Query: 121 DKSEKRYGQSPTRTATRSTWELSAENYAGRDIDSTDEIIQGRRIRVDNEEEERNDDNERR 180
           D+ EKRYG+ P  +A RSTWE+SAE +A R I+S DE  + RR R+DNEE+E NDD ERR
Sbjct: 125 DEPEKRYGKEPIGSANRSTWEVSAEKFARRGIESADE-RRERRTRMDNEEQE-NDDIERR 184

Query: 181 GSYEEVEDEEAEAIADPRWDKIKSRFRGTVDENAAPERPEFKRWNKQESWGRKTWREASE 240
           G+YEE EDEE EAIADPRWDKIKSRFRG VDE  A ERP+F+RWNKQESWGRKTW+EASE
Sbjct: 185 GNYEEGEDEEIEAIADPRWDKIKSRFRGMVDERGATERPQFQRWNKQESWGRKTWKEASE 244

Query: 241 STIPKMVGEGVYGVGPVLAALSAGRREFYALYMQEGLDLNNNNKKKKDKKGFEKVLRLAE 300
           ST+PKM+GEGVYGVGPVLAALSAGRREFYALYMQEG+DLNNNNKKKKDKKGFEKVL+LAE
Sbjct: 245 STLPKMIGEGVYGVGPVLAALSAGRREFYALYMQEGIDLNNNNKKKKDKKGFEKVLKLAE 304

Query: 301 RNALSIKEISKHDLNMICDNRPHQGLVLDASPLEMVKIKELDPVSLEEDKGSLWLALDEV 360
           +NAL+IKEISKHDLNMI DNRPHQGLVLD SPLEMVKIKELDPVSLE+DKGSLWLALDEV
Sbjct: 305 KNALTIKEISKHDLNMISDNRPHQGLVLDTSPLEMVKIKELDPVSLEDDKGSLWLALDEV 364

Query: 361 TDPQNLGAIIRSAYFFGASGVVLCAKNSAPLSGVVSKASAGSLELMELRYCTNMMQFLTS 420
           TDPQNLGAIIRSAYFFGASGVVLCAKNSAPLSGVVSKASAGSLELMELRYC NMMQFLTS
Sbjct: 365 TDPQNLGAIIRSAYFFGASGVVLCAKNSAPLSGVVSKASAGSLELMELRYCKNMMQFLTS 424

Query: 421 SAENGWRVLGASVSSKSLDLKEVQPGAPTILVLGSEGTGLRPLVERSCSQLVRIPGNIPV 480
           SA NGWRVLGASVSSKS+DL+EV PGAPTILVLGSEGTGLRPLVERSCSQLVRIPGNIP 
Sbjct: 425 SAGNGWRVLGASVSSKSIDLEEVLPGAPTILVLGSEGTGLRPLVERSCSQLVRIPGNIPA 484

Query: 481 DVTMEKDNDAEPAQPTNGCSIDEFRSFMAVESLNVSVAAGVLVHHLIGSNYINDPLET 525
           +VT++K+ND E    T+GCS+DEFRSFMAVESLNVSVAAGV VHHLIG++Y N+ LET
Sbjct: 485 NVTIDKENDIE---TTDGCSVDEFRSFMAVESLNVSVAAGVFVHHLIGNSYKNESLET 526

[Close]

BLAST of MS003866 vs. NCBI nr
Match: KAG6586465.1 (rRNA methyltransferase 1, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 754.2 bits (1946), Expect = 7.5e-214
Identity = 401/532 (75.38%), Postives = 448/532 (84.21%), Query Frame = 0


Query: 1   MKLSVRSRHCFPEIRELVKPELSSTRCL--SLGKFRSFSSSHPLKDEGVRSGNKLPWLA- 60
           M LS+RS HC PEIR+LV P+LSS      S  K R++SSSH +KDEGV+S +KLPWLA 
Sbjct: 1   MNLSIRSHHCSPEIRKLVSPKLSSPALTIESSLKSRTYSSSHSIKDEGVKSRDKLPWLAA 60

Query: 61  -----KSKWSEKSSGKTGRDQSSWKDSDTRSGKMRSSWGNPAKTFGKKSNRSSGDKSEKR 120
                K++W  KS+ K   D+SSWK+S++ S KMRSSW    KTFGK+ NR+SGD+ EKR
Sbjct: 61  TNSFEKARWLGKSTTKKRGDRSSWKESNSGSEKMRSSWDYSKKTFGKRGNRASGDEPEKR 120

Query: 121 YGQSPTRTATRSTWELSAENYAGRDIDSTDEIIQGRRIRVDNEEEERNDDNERRGSYEEV 180
           YG++P  +  RS+WE+SAE +A + ++  +E  + RR RVDNEE++ +D         EV
Sbjct: 121 YGKAPVGSTDRSSWEVSAEKFATQRMEYAEERRESRRGRVDNEEQQNHD--------VEV 180

Query: 181 EDEEAEAIADPRWDKIKSRFRGTVDENAAPERPEFKRWNKQESWGRKTWREASESTIPKM 240
           EDEE EAIADPRWDKIKS+F          ERPEF+RWNKQESW RKTW+EASEST+PKM
Sbjct: 181 EDEETEAIADPRWDKIKSKF----------ERPEFQRWNKQESWSRKTWKEASESTLPKM 240

Query: 241 VGEGVYGVGPVLAALSAGRREFYALYMQEGLDLNNNNKKKKDKKGFEKVLRLAERNALSI 300
           VGEGVYGVGPVLAALSAGRREFYALYMQEGLDLNNNNKKKKDKKGFEKVLRLAERNAL++
Sbjct: 241 VGEGVYGVGPVLAALSAGRREFYALYMQEGLDLNNNNKKKKDKKGFEKVLRLAERNALTV 300

Query: 301 KEISKHDLNMICDNRPHQGLVLDASPLEMVKIKELDPVSLEEDKGSLWLALDEVTDPQNL 360
           KEISKHDLNM+ DNRPHQGLVLDASPLEMVKI ELDPVSLEEDKGSLWLALDEVTDPQNL
Sbjct: 301 KEISKHDLNMLSDNRPHQGLVLDASPLEMVKIMELDPVSLEEDKGSLWLALDEVTDPQNL 360

Query: 361 GAIIRSAYFFGASGVVLCAKNSAPLSGVVSKASAGSLELMELRYCTNMMQFLTSSAENGW 420
           GAIIRSAYFFGASGVVLCAKNSAPLSGVVSKASAGSLELMELRYC NMMQFLTSSAENGW
Sbjct: 361 GAIIRSAYFFGASGVVLCAKNSAPLSGVVSKASAGSLELMELRYCKNMMQFLTSSAENGW 420

Query: 421 RVLGASVSSKSLDLKEVQPGAPTILVLGSEGTGLRPLVERSCSQLVRIPGNIPVDVTMEK 480
           RVLGASVSSKS+DL+EV PGAPTILVLGSEGTGLRPLVERSCSQLV+IPGNIPVD+ M+K
Sbjct: 421 RVLGASVSSKSIDLEEVPPGAPTILVLGSEGTGLRPLVERSCSQLVKIPGNIPVDIAMDK 480

Query: 481 DNDAEPAQPTNGCSIDEFRSFMAVESLNVSVAAGVLVHHLIGSNYINDPLET 525
           +ND E  QPT   S+DEFRSFMAVESLNVSVAAGVLVHHLIGSNY N+  ET
Sbjct: 481 ENDIETTQPTKESSVDEFRSFMAVESLNVSVAAGVLVHHLIGSNYKNESYET 514

[Close]

BLAST of MS003866 vs. ExPASy Swiss-Prot
Match: Q76NT0 (rRNA methyltransferase 1, mitochondrial OS=Dictyostelium discoideum OX=44689 GN=mrm1 PE=3 SV=1)

HSP 1 Score: 148.7 bits (374), Expect = 1.9e-34
Identity = 95/294 (32.31%), Postives = 149/294 (50.68%), Query Frame = 0


Query: 260 QEGLDLNNNNKKKK-------DKK---GFEKVLRLAERNALSIKEISKHDLNMICDNRPH 319
           +E  D NNNN  KK       D+K     +++++ + +  + ++ ++K  L+     RPH
Sbjct: 262 EEEEDNNNNNNIKKTTFNRYNDRKNLVALKEIVKNSFKLKIPVRSVNKGTLDSFSKGRPH 321

Query: 320 QGLVLDASPLEMVKIKELDPVSLEE-----------------------DKGSLWLALDEV 379
           QG++LDASPL ++ I  L+   + E                       DK  LWL LDE+
Sbjct: 322 QGIILDASPLTLLNIDFLEKFDINERVNKRMNGGGGGGGGGGGNKINNDKYPLWLVLDEL 381

Query: 380 TDPQNLGAIIRSAYFFGASGVVLCAKNSAPLSGVVSKASAGSLELMELRYCTNMMQFLTS 439
            DPQN+GAIIR+  FF   GVV+  KNS+P++   SK+S+G+ E   +    ++  FL S
Sbjct: 382 WDPQNVGAIIRNCSFFNIDGVVISNKNSSPITPAASKSSSGACESFVINRTESIEGFLKS 441

Query: 440 SAENGWRVLGASV-----SSKSLDLKEVQPGAPTILVLGSEGTGLRPLVERSCSQLVRIP 499
           S  NGW V+G S+     +   LD+ +++   PTIL+LG+EG GL+P V   C++ ++I 
Sbjct: 442 SQRNGWNVVGTSLDDLNENQPCLDINQIKLDQPTILILGNEGFGLKPSVLEICNKTIKIV 501

Query: 500 GNIPVDVTMEKDNDAEPAQPTNGCSIDEFRSFMAVESLNVSVAAGVLVHHLIGS 516
           G                                 ++SLNVSV +G+L+H L+ S
Sbjct: 502 G-----------------------------GNYKIDSLNVSVTSGILIHTLLSS 526

[Close]

BLAST of MS003866 vs. ExPASy Swiss-Prot
Match: Q6IN84 (rRNA methyltransferase 1, mitochondrial OS=Homo sapiens OX=9606 GN=MRM1 PE=1 SV=1)

HSP 1 Score: 139.8 bits (351), Expect = 8.8e-32
Identity = 97/310 (31.29%), Postives = 149/310 (48.06%), Query Frame = 0


Query: 217 GRKTWREASESTIPKMVGEGVYGVGPVLAALSAGRREFYALYMQEGLDLNNNNKKKKDKK 276
           G +  R   +  +P    E ++G+ P L AL A RR    L +Q G            K 
Sbjct: 32  GEELSRLLLDDLVPTSRLELLFGMTPCLLALQAARRSVARLLLQAG------------KA 91

Query: 277 GFE----KVLRLAERNALSIKEISKHDLNMICDNRPHQGLVLDASPLEMVKIKELDPVSL 336
           G +    ++LR+AE   + +    +  L+ +C  + HQG+ ++ SPL     +E    S 
Sbjct: 92  GLQGKRAELLRMAEARDIPVLRPRRQKLDTMCRYQVHQGVCMEVSPLRPRPWREAGEASP 151

Query: 337 EEDKGSLWLALDEVTDPQNLGAIIRSAYFFGASGVVLCAKNSAPLSGVVSKASAGSLELM 396
            +D   LWL LD + DP+N GA++RSA+F G   V+   +NS PL+ VVSK+SAG++E+M
Sbjct: 152 GDDPQQLWLVLDGIQDPRNFGAVLRSAHFLGVDKVITSRRNSCPLTPVVSKSSAGAMEVM 211

Query: 397 ELRYCTNMMQFLTSSAENGWRVLGA----------SVSSKSLDLKEVQPGAPTILVLGSE 456
           ++    ++  FL + A+ GW V G           S     +   E     PT+LVLG+E
Sbjct: 212 DVFSTDDLTGFLQTKAQQGWLVAGTVGCPSTEDPQSSEIPIMSCLEFLWERPTLLVLGNE 271

Query: 457 GTGLRPLVERSCSQLVRIPGNIPVDVTMEKDNDAEPAQPTNGCSIDEFRSFMAVESLNVS 513
           G+GL   V+ SC  L+ I                          +   +    +ESLNVS
Sbjct: 272 GSGLSQEVQASCQLLLTI--------------------------LPRRQLPPGLESLNVS 303

[Close]

BLAST of MS003866 vs. ExPASy Swiss-Prot
Match: Q99J25 (rRNA methyltransferase 1, mitochondrial OS=Mus musculus OX=10090 GN=Mrm1 PE=2 SV=2)

HSP 1 Score: 139.4 bits (350), Expect = 1.1e-31
Identity = 101/310 (32.58%), Postives = 148/310 (47.74%), Query Frame = 0


Query: 217 GRKTWREASESTIPKMVGEGVYGVGPVLAALSAGRREFYALYMQEGLDLNNNNKKKKDKK 276
           G +  R   +   P    E ++G+ P L AL A RR    L +Q G            K 
Sbjct: 30  GEELSRLLLDDLAPAQRLERLFGLSPCLLALRAARRRVARLLLQAG------------KA 89

Query: 277 GFE----KVLRLAERNALSIKEISKHDLNMICDNRPHQGLVLDASPLEMVKIKELDPVSL 336
           G +    ++LR+AE   + +    +  L+ +C  + HQG+ ++ SPL      E    S 
Sbjct: 90  GLQGERAELLRVAEARGIPVLRPRRQKLDALCGYQVHQGVCMEVSPLRPRPCDEAADTSS 149

Query: 337 EEDKGSLWLALDEVTDPQNLGAIIRSAYFFGASGVVLCAKNSAPLSGVVSKASAGSLELM 396
            +D   LWL L+ + DP+NLGA++RSA+F G   V+   +NS PL+ VVSKASAG++E+M
Sbjct: 150 GDDPQQLWLVLEGLQDPRNLGAVMRSAHFLGVDRVITSQRNSCPLTPVVSKASAGAMEVM 209

Query: 397 ELRYCTNMMQFLTSSAENGWRVLGA--------SVSSK--SLDLKEVQPGAPTILVLGSE 456
           ++    ++  FL + A+ GW V+G         S SSK       E     PT+LVLGSE
Sbjct: 210 DVFATPDLPGFLQAKAQQGWLVVGTVGCPGPEISQSSKVPITSCLEFVWDRPTLLVLGSE 269

Query: 457 GTGLRPLVERSCSQLVRIPGNIPVDVTMEKDNDAEPAQPTNGCSIDEFRSFMAVESLNVS 513
           G+GL   V  SC  L+ I                          +        +ESLNVS
Sbjct: 270 GSGLSQEVFASCQLLLTI--------------------------LPRRHLPPGLESLNVS 301

[Close]

BLAST of MS003866 vs. ExPASy Swiss-Prot
Match: O94631 (rRNA methyltransferase 1, mitochondrial OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=mrm1 PE=3 SV=1)

HSP 1 Score: 136.7 bits (343), Expect = 7.4e-31
Identity = 110/321 (34.27%), Postives = 155/321 (48.29%), Query Frame = 0


Query: 224 ASESTIPKMVGEGVYGVGPVLAALSAGRREFYALYMQEGLDLNNNNKKKKDKKGFEKVLR 283
           + E  + K   E V+G   V  AL+  +RE  ALY+Q   D+++          FE+ L 
Sbjct: 21  SKERRLIKEDSEFVFGTHSVKNALATRKRECRALYVQNA-DIHSE---------FEEFLN 80

Query: 284 LAERNALSIKEISKHDLNMICDNRPHQGLVLDASPLEMVKIKEL---------------- 343
             +   + IK ++K  LN I   RPH G+VL+AS L +  I +L                
Sbjct: 81  KLQYK-IPIKSVNKEHLNQITACRPHNGVVLEASSLNVPTISDLLFPAGEEYNNKNGQDS 140

Query: 344 DPVSLEEDKGS-------LWLALDEVTDPQNLGAIIRSAYFFGASGVVLCAKNSAPLSGV 403
               L E K S       L++ +D +TDPQN+GA+IRSAY  GA G++L  K++  LS V
Sbjct: 141 PHNDLNEGKSSSSDNYPPLYVYVDGITDPQNMGAVIRSAYILGAKGILLSKKHNTFLSPV 200

Query: 404 VSKASAGSLELMELRYCTNMMQFLTSSAENGWRVLGASVSSKS--------LDLKEVQPG 463
           VSKASAG+LE+  + +  N M FL +S   GW+V+G   +             +K     
Sbjct: 201 VSKASAGALEVFNISHVKNPMVFLRNSVLKGWKVIGTKPALPDNKDQIYTPHKIKTELMN 260

Query: 464 APTILVLGSEGTGLRPLVERSCSQLVRIPGNIPVDVTMEKDNDAEPAQPTNGCSIDEFRS 514
            P ILVLGSE  GLR  +   CS  V IPG                         D++  
Sbjct: 261 EPKILVLGSE-KGLRTNILTQCSHYVSIPGG------------------------DKY-- 300

[Close]

BLAST of MS003866 vs. ExPASy Swiss-Prot
Match: A4T5L0 (Uncharacterized tRNA/rRNA methyltransferase Mflv_1447 OS=Mycolicibacterium gilvum (strain PYR-GCK) OX=350054 GN=Mflv_1447 PE=3 SV=1)

HSP 1 Score: 113.2 bits (282), Expect = 8.8e-24
Identity = 104/349 (29.80%), Postives = 160/349 (45.85%), Query Frame = 0


Query: 162 DNERRGSYEEVEDEEAEAIADPRWDKIKSRFRG-TVDENAAPERPEFKRWNKQESWGRKT 221
           +++RRG+  +   ++   +      +     RG T   +  P  P  KR  K     +  
Sbjct: 4   NSQRRGAVRKAGTKKGPQVGSGGVRRRGLEGRGATPPAHERPHHPAGKRAAKAARQAQGR 63

Query: 222 WREASESTIPKMVGEGVYGVGPVLAALSAGRREFYALYMQEGLDLNNNNKKKKDKKGFEK 281
            ++  ++ I       V G  PV+  L AG     ALY+  G D         D++  E 
Sbjct: 64  HKKTDDTEI-------VLGRNPVVECLRAG-VPATALYVALGTD--------ADERLTES 123

Query: 282 VLRLAERNALSIKEISKHDLNMICDNRPHQGLVLDASPLEMVKIKELDPVSLEEDKGSLW 341
           V   A+R  +SI E+ +HDL+ +  N  HQG+ L   P       +L   +  +   +L 
Sbjct: 124 VQMAADR-GISILEVQRHDLDRMAANGLHQGIALQVPPYAYAHPDDLLRDAKSDAAPALL 183

Query: 342 LALDEVTDPQNLGAIIRSAYFFGASGVVLCAKNSAPLSGVVSKASAGSLELMELRYCTNM 401
           +ALD ++DP+NLGAI+RS   FG  GVVL  + SA ++ V  + SAG+     +   TN+
Sbjct: 184 VALDNISDPRNLGAIVRSVAAFGGHGVVLPQRRSASVTAVAWRTSAGAAARTPVARATNL 243

Query: 402 MQFLTSSAENGWRVLGASVSSKSLDLKEVQPGAPTILVLGSEGTGLRPLVERSCSQLVRI 461
            + L   A+ G +V+G      +  + E+    PTI+V+GSEG GL  LV  +C ++V I
Sbjct: 244 NRTLKQYADAGLQVVGLDAGGDT-TIDEIDGLTPTIVVVGSEGKGLSRLVRENCDRIVSI 303

Query: 462 PGNIPVDVTMEKDNDAEPAQPTNGCSIDEFRSFMAVESLNVSVAAGVLV 510
           P                 A PT              ESLN SVAAGV++
Sbjct: 304 P----------------MAGPT--------------ESLNASVAAGVVL 304

[Close]

BLAST of MS003866 vs. ExPASy TrEMBL
Match: A0A6J1CVE3 (uncharacterized protein LOC111014564 OS=Momordica charantia OX=3673 GN=LOC111014564 PE=4 SV=1)

HSP 1 Score: 982.2 bits (2538), Expect = 8.2e-283
Identity = 507/513 (98.83%), Postives = 508/513 (99.03%), Query Frame = 0


Query: 1   MKLSVRSRHCFPEIRELVKPELSSTRCLSLGKFRSFSSSHPLKDEGVRSGNKLPWLAKSK 60
           MKLSVRSRHCFPEIRELVKPELSSTRCLSLGKFRSFSSSHPLKDEGVRSGNKLPWLAKSK
Sbjct: 59  MKLSVRSRHCFPEIRELVKPELSSTRCLSLGKFRSFSSSHPLKDEGVRSGNKLPWLAKSK 118

Query: 61  WSEKSSGKTGRDQSSWKDSDTRSGKMRSSWGNPAKTFGKKSNRSSGDKSEKRYGQSPTRT 120
           WSEKSSGKTGRDQSSWKDSDTRSGKMRSSWGNPAKTFGKKSNRSSGDKSEKRYGQSPTRT
Sbjct: 119 WSEKSSGKTGRDQSSWKDSDTRSGKMRSSWGNPAKTFGKKSNRSSGDKSEKRYGQSPTRT 178

Query: 121 ATRSTWELSAENYAGRDIDSTDEIIQGRRIRVDNEEEERNDDNERRGSYEEVEDEEAEAI 180
           ATRSTWELSAENYAGRDIDSTDEIIQGRRIRVDN EEERNDDNERRGSYEEVEDEEAEAI
Sbjct: 179 ATRSTWELSAENYAGRDIDSTDEIIQGRRIRVDN-EEERNDDNERRGSYEEVEDEEAEAI 238

Query: 181 ADPRWDKIKSRFRGTVDENAAPERPEFKRWNKQESWGRKTWREASESTIPKMVGEGVYGV 240
           ADPRWDKIKSRFRGTVDENAAPERPEFKRWNKQESWGRKTWREASESTIPKMVGEGVYGV
Sbjct: 239 ADPRWDKIKSRFRGTVDENAAPERPEFKRWNKQESWGRKTWREASESTIPKMVGEGVYGV 298

Query: 241 GPVLAALSAGRREFYALYMQEGLDLNNNNKKKKDKKGFEKVLRLAERNALSIKEISKHDL 300
           GPVLAALSAGRREFYALYMQEGLDL NNNK KKDKKGFEKVLRLAERNALSIKEISKHDL
Sbjct: 299 GPVLAALSAGRREFYALYMQEGLDLKNNNKTKKDKKGFEKVLRLAERNALSIKEISKHDL 358

Query: 301 NMICDNRPHQGLVLDASPLEMVKIKELDPVSLEEDKGSLWLALDEVTDPQNLGAIIRSAY 360
           NMICDNRPHQGLVLDASPLEMVKIKELDPVSLEEDKGSLWLALDEVTDPQNLGAIIRSAY
Sbjct: 359 NMICDNRPHQGLVLDASPLEMVKIKELDPVSLEEDKGSLWLALDEVTDPQNLGAIIRSAY 418

Query: 361 FFGASGVVLCAKNSAPLSGVVSKASAGSLELMELRYCTNMMQFLTSSAENGWRVLGASVS 420
           FFGASGVVLCAKNSAPLSGVVSKASAGSLELMELRYC NMMQFLTSSAENGWRVLGASVS
Sbjct: 419 FFGASGVVLCAKNSAPLSGVVSKASAGSLELMELRYCKNMMQFLTSSAENGWRVLGASVS 478

Query: 421 SKSLDLKEVQPGAPTILVLGSEGTGLRPLVERSCSQLVRIPGNIPVDVTMEKDNDAEPAQ 480
           SKSLDLKEVQPGAPTILVLGSEGTGLRPLVERSCSQLVR+PGNIPVDVTMEKDNDAEPAQ
Sbjct: 479 SKSLDLKEVQPGAPTILVLGSEGTGLRPLVERSCSQLVRVPGNIPVDVTMEKDNDAEPAQ 538

Query: 481 PTNGCSIDEFRSFMAVESLNVSVAAGVLVHHLI 514
            TNGCSIDEFRSFMAVESLNVSVAAGVLVHHLI
Sbjct: 539 TTNGCSIDEFRSFMAVESLNVSVAAGVLVHHLI 570

[Close]

BLAST of MS003866 vs. ExPASy TrEMBL
Match: A0A0A0LW97 (SpoU_sub_bind domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G574920 PE=4 SV=1)

HSP 1 Score: 783.1 bits (2021), Expect = 7.3e-223
Identity = 416/540 (77.04%), Postives = 460/540 (85.19%), Query Frame = 0


Query: 1   MKLSVRSRHCFPEIRELVKPEL--------SSTRCLSLGKFRSFSSSHPLKDEGVRSGNK 60
           +KLS+R RHCFPEIR+LV P+L        SS R +SL K R FSSS+ LKDEGV SGNK
Sbjct: 5   VKLSIRGRHCFPEIRDLVSPQLNSLPLSVGSSLRSVSLNKSRCFSSSYRLKDEGVLSGNK 64

Query: 61  LPWLA------KSKWSEKSSGKTGRDQSSWKDSDTRSGKMRSSWGNPAKTFGKKSNRSSG 120
           LPWLA      K+KW +KS+ K   D+S WK+SD +S           K FGK  NRSS 
Sbjct: 65  LPWLAAASSVEKAKWLDKSTTKKRGDRSPWKESDFKS-----------KRFGKNGNRSSR 124

Query: 121 DKSEKRYGQSPTRTATRSTWELSAENYAGRDIDSTDEIIQGRRIRVDNEEEERNDDNERR 180
           D+ EKRYG+ P  +A RSTWE+SAE +A R I+  DE  + RR R+DN EE+++DD ERR
Sbjct: 125 DEPEKRYGKEPIGSANRSTWEVSAEKFARRSIELADEKRESRRTRMDN-EEQKDDDIERR 184

Query: 181 GSYEEVEDEEAEAIADPRWDKIKSRFRGTVDENAAPERPEFKRWNKQESWGRKTWREASE 240
           GSYEEVEDEE EAIADPRWDKIKSRFRG VDE  A ERPEF+RWNKQESWGRKTW+EASE
Sbjct: 185 GSYEEVEDEETEAIADPRWDKIKSRFRGMVDERGATERPEFQRWNKQESWGRKTWKEASE 244

Query: 241 STIPKMVGEGVYGVGPVLAALSAGRREFYALYMQEGLDLNNNNKKKKDKKGFEKVLRLAE 300
           ST+PKM+GEGVYGVGPVLAALSAGRREFYALY+QEG+DLNNNNKKKKDKKGFEKVL+LAE
Sbjct: 245 STLPKMIGEGVYGVGPVLAALSAGRREFYALYVQEGIDLNNNNKKKKDKKGFEKVLKLAE 304

Query: 301 RNALSIKEISKHDLNMICDNRPHQGLVLDASPLEMVKIKELDPVSLEEDKGSLWLALDEV 360
           +NAL+IKEISKHDLNMI DNRPHQGLVLDASPLEMVKIKELDPVSLE+DKGSLWLALDEV
Sbjct: 305 KNALTIKEISKHDLNMISDNRPHQGLVLDASPLEMVKIKELDPVSLEDDKGSLWLALDEV 364

Query: 361 TDPQNLGAIIRSAYFFGASGVVLCAKNSAPLSGVVSKASAGSLELMELRYCTNMMQFLTS 420
           TDPQNLGAIIRSAYFFGASGVVLCAKNSAPLSGVVSKASAGSLELMELRYC NMMQFLTS
Sbjct: 365 TDPQNLGAIIRSAYFFGASGVVLCAKNSAPLSGVVSKASAGSLELMELRYCKNMMQFLTS 424

Query: 421 SAENGWRVLGASVSSKSLDLKEVQPGAPTILVLGSEGTGLRPLVERSCSQLVRIPGNIPV 480
           SAENGWRVLGASVSSKS+DLKEV PGAPTILVLGSEGTGLRPLVERSCSQLVRIPGNIPV
Sbjct: 425 SAENGWRVLGASVSSKSIDLKEVLPGAPTILVLGSEGTGLRPLVERSCSQLVRIPGNIPV 484

Query: 481 DVTMEKDNDAEPAQPTNGCSIDEFRSFMAVESLNVSVAAGVLVHHLIGSNYINDPLETCV 527
           DVT++ +ND E    T+G S+DEFRSFMAVESLNVSVAAGV VHHL+G++Y N+ LET +
Sbjct: 485 DVTIDTENDIE---TTDGRSVDEFRSFMAVESLNVSVAAGVFVHHLVGNSYKNESLETSI 529

[Close]

BLAST of MS003866 vs. ExPASy TrEMBL
Match: A0A5D3CIS9 (Putative tRNA/rRNA methyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G002930 PE=4 SV=1)

HSP 1 Score: 778.1 bits (2008), Expect = 2.3e-221
Identity = 416/538 (77.32%), Postives = 459/538 (85.32%), Query Frame = 0


Query: 1   MKLSVRSRHCFPEIRELVKPEL--------SSTRCLSLGKFRSFSSSHPLKDEGVRSGNK 60
           +KLS+R RHCFPEIR+LV P+L        SS + LSL K R FSSS+ LKDEGV SGNK
Sbjct: 5   VKLSIRGRHCFPEIRDLVSPQLNSLPLSVGSSLKSLSLNKSRCFSSSYRLKDEGVLSGNK 64

Query: 61  LPWLA------KSKWSEKSSGKTGRDQSSWKDSDTRSGKMRSSWGNPAKTFGKKSNRSSG 120
           LPWLA      K+KW +KS+ K   D+SSWK+SD +S           K FGK  NRSS 
Sbjct: 65  LPWLAAANSVEKAKWLDKSTTKKRGDRSSWKESDFKS-----------KRFGKNGNRSSR 124

Query: 121 DKSEKRYGQSPTRTATRSTWELSAENYAGRDIDSTDEIIQGRRIRVDNEEEERNDDNERR 180
           D+ EKRYG+ P  +A RSTWE+SAE +A R I+S DE  + RR R+DNEE+E NDD ERR
Sbjct: 125 DEPEKRYGKEPIGSANRSTWEVSAEKFARRGIESADE-RRERRTRMDNEEQE-NDDIERR 184

Query: 181 GSYEEVEDEEAEAIADPRWDKIKSRFRGTVDENAAPERPEFKRWNKQESWGRKTWREASE 240
           G+YEE EDEE EAIADPRWDKIKSRFRG VDE  A ERP+F+RWNKQESWGRKTW+EASE
Sbjct: 185 GNYEEGEDEEIEAIADPRWDKIKSRFRGMVDERGATERPQFQRWNKQESWGRKTWKEASE 244

Query: 241 STIPKMVGEGVYGVGPVLAALSAGRREFYALYMQEGLDLNNNNKKKKDKKGFEKVLRLAE 300
           ST+PKM+GEGVYGVGPVLAALSAGRREFYALYMQEG+DLNNNNKKKKDKKGFEKVL+LAE
Sbjct: 245 STLPKMIGEGVYGVGPVLAALSAGRREFYALYMQEGIDLNNNNKKKKDKKGFEKVLKLAE 304

Query: 301 RNALSIKEISKHDLNMICDNRPHQGLVLDASPLEMVKIKELDPVSLEEDKGSLWLALDEV 360
           +NAL+IKEISKHDLNMI DNRPHQGLVLD SPLEMVKIKELDPVSLE+DKGSLWLALDEV
Sbjct: 305 KNALTIKEISKHDLNMISDNRPHQGLVLDTSPLEMVKIKELDPVSLEDDKGSLWLALDEV 364

Query: 361 TDPQNLGAIIRSAYFFGASGVVLCAKNSAPLSGVVSKASAGSLELMELRYCTNMMQFLTS 420
           TDPQNLGAIIRSAYFFGASGVVLCAKNSAPLSGVVSKASAGSLELMELRYC NMMQFLTS
Sbjct: 365 TDPQNLGAIIRSAYFFGASGVVLCAKNSAPLSGVVSKASAGSLELMELRYCKNMMQFLTS 424

Query: 421 SAENGWRVLGASVSSKSLDLKEVQPGAPTILVLGSEGTGLRPLVERSCSQLVRIPGNIPV 480
           SA NGWRVLGASVSSKS+DL+EV PGAPTILVLGSEGTGLRPLVERSCSQLVRIPGNIP 
Sbjct: 425 SAGNGWRVLGASVSSKSIDLEEVLPGAPTILVLGSEGTGLRPLVERSCSQLVRIPGNIPA 484

Query: 481 DVTMEKDNDAEPAQPTNGCSIDEFRSFMAVESLNVSVAAGVLVHHLIGSNYINDPLET 525
           +VT++K+ND E    T+GCS+DEFRSFMAVESLNVSVAAGV VHHLIG++Y N+ LET
Sbjct: 485 NVTIDKENDIE---TTDGCSVDEFRSFMAVESLNVSVAAGVFVHHLIGNSYKNESLET 526

[Close]

BLAST of MS003866 vs. ExPASy TrEMBL
Match: A0A1S3BQ13 (uncharacterized tRNA/rRNA methyltransferase MSMEG_6073/MSMEI_5913 OS=Cucumis melo OX=3656 GN=LOC103492262 PE=4 SV=1)

HSP 1 Score: 778.1 bits (2008), Expect = 2.3e-221
Identity = 416/538 (77.32%), Postives = 459/538 (85.32%), Query Frame = 0


Query: 1   MKLSVRSRHCFPEIRELVKPEL--------SSTRCLSLGKFRSFSSSHPLKDEGVRSGNK 60
           +KLS+R RHCFPEIR+LV P+L        SS + LSL K R FSSS+ LKDEGV SGNK
Sbjct: 5   VKLSIRGRHCFPEIRDLVSPQLNSLPLSVGSSLKSLSLNKSRCFSSSYRLKDEGVLSGNK 64

Query: 61  LPWLA------KSKWSEKSSGKTGRDQSSWKDSDTRSGKMRSSWGNPAKTFGKKSNRSSG 120
           LPWLA      K+KW +KS+ K   D+SSWK+SD +S           K FGK  NRSS 
Sbjct: 65  LPWLAAANSVEKAKWLDKSTTKKRGDRSSWKESDFKS-----------KRFGKNGNRSSR 124

Query: 121 DKSEKRYGQSPTRTATRSTWELSAENYAGRDIDSTDEIIQGRRIRVDNEEEERNDDNERR 180
           D+ EKRYG+ P  +A RSTWE+SAE +A R I+S DE  + RR R+DNEE+E NDD ERR
Sbjct: 125 DEPEKRYGKEPIGSANRSTWEVSAEKFARRGIESADE-RRERRTRMDNEEQE-NDDIERR 184

Query: 181 GSYEEVEDEEAEAIADPRWDKIKSRFRGTVDENAAPERPEFKRWNKQESWGRKTWREASE 240
           G+YEE EDEE EAIADPRWDKIKSRFRG VDE  A ERP+F+RWNKQESWGRKTW+EASE
Sbjct: 185 GNYEEGEDEEIEAIADPRWDKIKSRFRGMVDERGATERPQFQRWNKQESWGRKTWKEASE 244

Query: 241 STIPKMVGEGVYGVGPVLAALSAGRREFYALYMQEGLDLNNNNKKKKDKKGFEKVLRLAE 300
           ST+PKM+GEGVYGVGPVLAALSAGRREFYALYMQEG+DLNNNNKKKKDKKGFEKVL+LAE
Sbjct: 245 STLPKMIGEGVYGVGPVLAALSAGRREFYALYMQEGIDLNNNNKKKKDKKGFEKVLKLAE 304

Query: 301 RNALSIKEISKHDLNMICDNRPHQGLVLDASPLEMVKIKELDPVSLEEDKGSLWLALDEV 360
           +NAL+IKEISKHDLNMI DNRPHQGLVLD SPLEMVKIKELDPVSLE+DKGSLWLALDEV
Sbjct: 305 KNALTIKEISKHDLNMISDNRPHQGLVLDTSPLEMVKIKELDPVSLEDDKGSLWLALDEV 364

Query: 361 TDPQNLGAIIRSAYFFGASGVVLCAKNSAPLSGVVSKASAGSLELMELRYCTNMMQFLTS 420
           TDPQNLGAIIRSAYFFGASGVVLCAKNSAPLSGVVSKASAGSLELMELRYC NMMQFLTS
Sbjct: 365 TDPQNLGAIIRSAYFFGASGVVLCAKNSAPLSGVVSKASAGSLELMELRYCKNMMQFLTS 424

Query: 421 SAENGWRVLGASVSSKSLDLKEVQPGAPTILVLGSEGTGLRPLVERSCSQLVRIPGNIPV 480
           SA NGWRVLGASVSSKS+DL+EV PGAPTILVLGSEGTGLRPLVERSCSQLVRIPGNIP 
Sbjct: 425 SAGNGWRVLGASVSSKSIDLEEVLPGAPTILVLGSEGTGLRPLVERSCSQLVRIPGNIPA 484

Query: 481 DVTMEKDNDAEPAQPTNGCSIDEFRSFMAVESLNVSVAAGVLVHHLIGSNYINDPLET 525
           +VT++K+ND E    T+GCS+DEFRSFMAVESLNVSVAAGV VHHLIG++Y N+ LET
Sbjct: 485 NVTIDKENDIE---TTDGCSVDEFRSFMAVESLNVSVAAGVFVHHLIGNSYKNESLET 526

[Close]

BLAST of MS003866 vs. ExPASy TrEMBL
Match: A0A6J1HPM2 (uncharacterized protein LOC111465489 OS=Cucurbita maxima OX=3661 GN=LOC111465489 PE=4 SV=1)

HSP 1 Score: 746.1 bits (1925), Expect = 9.9e-212
Identity = 399/532 (75.00%), Postives = 442/532 (83.08%), Query Frame = 0


Query: 1   MKLSVRSRHCFPEIRELVKPELSSTRCL--SLGKFRSFSSSHPLKDEGVRSGNKLPWLA- 60
           M LS+RS HC PEIR+ V P+LSS      S  K R++SSSH +KDEGV+S NKLPWLA 
Sbjct: 55  MNLSMRSHHCSPEIRKFVSPKLSSPALTAESSLKSRTYSSSHSIKDEGVKSRNKLPWLAA 114

Query: 61  -----KSKWSEKSSGKTGRDQSSWKDSDTRSGKMRSSWGNPAKTFGKKSNRSSGDKSEKR 120
                K+KW +KS+ K   D+SSWK+S++ S KMRSSW    KTFGK+ NR SGD+ EKR
Sbjct: 115 TNSFEKAKWLDKSTTKKRGDRSSWKESNSGSEKMRSSWDYSKKTFGKRGNRPSGDEPEKR 174

Query: 121 YGQSPTRTATRSTWELSAENYAGRDIDSTDEIIQGRRIRVDNEEEERNDDNERRGSYEEV 180
           YG++P  +A RS+WE+SAE +  + ++  DE  + RR RVDNEE+   D         EV
Sbjct: 175 YGKAPVGSAYRSSWEVSAEKFGTQGMEYADERRESRRGRVDNEEQRNRD--------VEV 234

Query: 181 EDEEAEAIADPRWDKIKSRFRGTVDENAAPERPEFKRWNKQESWGRKTWREASESTIPKM 240
           EDEE EAIADPRWDKIKS+F          ER EF+RWNKQESW RKTW+EASEST+PKM
Sbjct: 235 EDEETEAIADPRWDKIKSKF----------ERHEFQRWNKQESWSRKTWKEASESTLPKM 294

Query: 241 VGEGVYGVGPVLAALSAGRREFYALYMQEGLDLNNNNKKKKDKKGFEKVLRLAERNALSI 300
           VGEGVYGVGPVLAALSAGRREFYALYMQEGLDLNNNNKKKKDKKGFEKVLRLAERNAL++
Sbjct: 295 VGEGVYGVGPVLAALSAGRREFYALYMQEGLDLNNNNKKKKDKKGFEKVLRLAERNALTV 354

Query: 301 KEISKHDLNMICDNRPHQGLVLDASPLEMVKIKELDPVSLEEDKGSLWLALDEVTDPQNL 360
           KEISKHDLNM+ DNRPHQGLVLDASPLEMVKI ELDPVSLEEDKGSLWLALDEVTDPQNL
Sbjct: 355 KEISKHDLNMLSDNRPHQGLVLDASPLEMVKIMELDPVSLEEDKGSLWLALDEVTDPQNL 414

Query: 361 GAIIRSAYFFGASGVVLCAKNSAPLSGVVSKASAGSLELMELRYCTNMMQFLTSSAENGW 420
           GAIIRSAYFFGASGVVLCAKNSAPLSGVVSKASAGSLELMELRYC NMMQFLTSSAENGW
Sbjct: 415 GAIIRSAYFFGASGVVLCAKNSAPLSGVVSKASAGSLELMELRYCKNMMQFLTSSAENGW 474

Query: 421 RVLGASVSSKSLDLKEVQPGAPTILVLGSEGTGLRPLVERSCSQLVRIPGNIPVDVTMEK 480
           RVLGASVSSKS+DL+EV PGAPTILVLGSEGTGLRPLVERSCSQLV+IPGNIPVDV +++
Sbjct: 475 RVLGASVSSKSIDLEEVPPGAPTILVLGSEGTGLRPLVERSCSQLVKIPGNIPVDVAIDR 534

Query: 481 DNDAEPAQPTNGCSIDEFRSFMAVESLNVSVAAGVLVHHLIGSNYINDPLET 525
           DND E  QPT   S+DEFRSFMAVESLNV VAAGVLVHHLIGS+Y  +  ET
Sbjct: 535 DNDIETTQPTKESSVDEFRSFMAVESLNVGVAAGVLVHHLIGSSYKKESYET 568

[Close]

BLAST of MS003866 vs. TAIR 10
Match: AT2G19870.1 (tRNA/rRNA methyltransferase (SpoU) family protein )

HSP 1 Score: 461.5 bits (1186), Expect = 9.3e-130
Identity = 281/545 (51.56%), Postives = 359/545 (65.87%), Query Frame = 0


Query: 5   VRSRHCFPEIRELVKPELSSTRCLSLGKFRSFSSSHPLKDEGVRSGNKLPWLAKSKWSEK 64
           V SR    +IR L  PE        + +F SF S    K     S      L    + +K
Sbjct: 57  VSSRLNSMKIRMLRNPE-------GVREFSSFDSQRFGKRSSSNSSRGKSGLGSKAYRDK 116

Query: 65  SSGKTGRD--QSSWKDSDTRSGKMRSSWGNP---AKTFGKKSNRSSGDKSEKRYGQSPTR 124
            SG +GR    S W  SD +  + R   G+     K  G+  + S      + YG++  R
Sbjct: 117 RSGGSGRSSGDSIWVKSDEKPVEERFQRGDRPSWEKRDGRNDSGSDRRSRSRGYGETRNR 176

Query: 125 TATR-------STWELSAENYAGRDI--DSTDEIIQGRRIRVDN----EEEERNDDN-ER 184
            + R       S  E  ++   G  I   + D+  + +  RV+N    ++  RN ++   
Sbjct: 177 DSFRGRRDDRISEVEEESKKGGGNSIWVANDDKPAKEQSPRVNNRSSWDDRTRNQNSFSA 236

Query: 185 RGSYEEVEDEEAEAI---------------ADPRWDKIKSRFRGTVDENAAPERPEFKRW 244
           RG     E+ E E +                + RW +IK+RF      +   +   ++ W
Sbjct: 237 RGDDRITEEAEEETMNHAPEDGDGIVEEEPDNTRWSEIKNRFNRYDVRDQGRDDAAYRNW 296

Query: 245 NKQESWGRKTWREASESTIPKMVGEGVYGVGPVLAALSAGRREFYALYMQEGLDLNNNNK 304
           N+QESWGRKTW+EA+ES++P++ GE VYGV PVLAALS GRREFYALY+QEGLDL++NN+
Sbjct: 297 NRQESWGRKTWQEATESSVPRLEGEVVYGVSPVLAALSVGRREFYALYVQEGLDLSSNNR 356

Query: 305 KKKDKKGFEKVLRLAERNALSIKEISKHDLNMICDNRPHQGLVLDASPLEMVKIKELDPV 364
           KKKDKKGFE+VL+++E+  L+IKE SKHDLNM+ DNRPHQGLVLDASPLE+VK+KELDP+
Sbjct: 357 KKKDKKGFERVLKISEKLGLNIKETSKHDLNMVADNRPHQGLVLDASPLELVKVKELDPI 416

Query: 365 SLEEDKGSLWLALDEVTDPQNLGAIIRSAYFFGASGVVLCAKNSAPLSGVVSKASAGSLE 424
           S EE+K SLW+ALDEVTDPQNLGAIIRSAYFFGA+GVV+CAKNSAPLS VVSKASAGSLE
Sbjct: 417 SSEEEKYSLWVALDEVTDPQNLGAIIRSAYFFGATGVVVCAKNSAPLSAVVSKASAGSLE 476

Query: 425 LMELRYCTNMMQFLTSSAENGWRVLGASVSSKSLDLKEVQPGAPTILVLGSEGTGLRPLV 484
           +MELRYC NMMQFL +SAENGWRV+G SVS K++ L EV PG+PTILVLG+EGTGLRPLV
Sbjct: 477 VMELRYCKNMMQFLEASAENGWRVVGGSVSPKAVALNEVLPGSPTILVLGNEGTGLRPLV 536

Query: 485 ERSCSQLVRIPGNIPVDVTMEKDNDAEPAQPTNGCSIDEFRSFMAVESLNVSVAAGVLVH 516
           ERSC+ LVRI GN+P +V + + +DAE          + FRSF+AVESLNVSVAAG+ +H
Sbjct: 537 ERSCTDLVRISGNMPNEVAVTESDDAEG---------EGFRSFLAVESLNVSVAAGLFLH 585

[Close]

BLAST of MS003866 vs. TAIR 10
Match: AT4G38020.1 (tRNA/rRNA methyltransferase (SpoU) family protein )

HSP 1 Score: 48.5 bits (114), Expect = 1.9e-05
Identity = 50/185 (27.03%), Postives = 71/185 (38.38%), Query Frame = 0


Query: 341 LALDEVTDPQNLGAIIRSAYFFGASGVVLCAKNSAPLSGVVSKASAGS------------ 400
           L LD + DP NLG ++RSA  F   G  L      P +    +AS G+            
Sbjct: 199 LVLDSIQDPGNLGTLVRSAMAFNWDGAFLLPGCCDPYNDKALRASRGASFQLPIVSGNWN 258

Query: 401 -LELMELRYCTNMMQFLTSSAENGWRVLGASVSSKSLDLKEVQPGAPTILVLGSEGTGLR 460
            L+L+E  +    M+ L        + L   VS  S++  +     P  L+LGSEG GL 
Sbjct: 259 HLKLLENEF---QMKLLAGHPATTTQKL-KPVSKLSVEFAQSLAEKPLCLILGSEGNGLS 318

Query: 461 PLVERSCSQLVRIPGNIPVDVTMEKDNDAEPAQPTNGCSIDEFRSFMAVESLNVSVAAGV 513
               + C         + V + M  D                       ESLNVSVA G+
Sbjct: 319 EQARKVC---------VLVSIPMAGD----------------------FESLNVSVAGGI 348

[Close]

BLAST of MS003866 vs. TAIR 10
Match: AT5G15390.1 (tRNA/rRNA methyltransferase (SpoU) family protein )

HSP 1 Score: 48.1 bits (113), Expect = 2.5e-05
Identity = 41/182 (22.53%), Postives = 78/182 (42.86%), Query Frame = 0


Query: 332 LEEDKGSLWLALDEVTDPQNLGAIIRSAYFFGASGVVLCAKNSAPLSGVVSKASAGSLEL 391
           +E    S+ L ++ ++D  N+ A  RSA   G   V + + +S+         S G+ + 
Sbjct: 148 VENRSYSVCLVVEGLSDFGNISAAFRSADALGIQSVHVVSCDSSKRYNGNRHVSMGAEKW 207

Query: 392 MELRYCTNMMQFLTSSAENGWRVLGASVSSKSLDLKEVQPGAPTILVLGSEGTGLRPLVE 451
           +++ +     +        G+R+    +   ++ + ++    PT +V+G+EG G+     
Sbjct: 208 LDIEFWDTPKECFKVLKSRGYRIATTHLGMDTVSIYDMDWSCPTAIVVGNEGRGIS---- 267

Query: 452 RSCSQLVRIPGNIPVDVTMEKDNDAEPAQPTNGCSIDEFRSFMAVESLNVSVAAGVLVHH 511
                          D  +E  +D   + P NG           V+S NVSVAAG+L+HH
Sbjct: 268 ---------------DEALEL-SDLRCSIPMNG----------MVDSFNVSVAAGILMHH 299

Query: 512 LI 514
            +
Sbjct: 328 AV 299

The following BLAST results are available for this feature:

BLAST of MS003866 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022145044.1	1.7e-282	98.83	uncharacterized protein LOC111014564 [Momordica charantia] >XP_022145045.1 uncha...	[more]
XP_038880007.1	3.5e-227	78.40	uncharacterized protein LOC120071698 [Benincasa hispida] >XP_038880008.1 unchara...	[more]
XP_004135672.1	1.5e-222	77.04	uncharacterized protein LOC101220884 [Cucumis sativus] >XP_031739747.1 uncharact...	[more]
XP_008450781.1	4.8e-221	77.32	PREDICTED: uncharacterized tRNA/rRNA methyltransferase MSMEG_6073/MSMEI_5913 [Cu...	[more]
KAG6586465.1	7.5e-214	75.38	rRNA methyltransferase 1, mitochondrial, partial [Cucurbita argyrosperma subsp. ...	[more]

BLAST of MS003866 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q76NT0	1.9e-34	32.31	rRNA methyltransferase 1, mitochondrial OS=Dictyostelium discoideum OX=44689 GN=...	[more]
Q6IN84	8.8e-32	31.29	rRNA methyltransferase 1, mitochondrial OS=Homo sapiens OX=9606 GN=MRM1 PE=1 SV=...	[more]
Q99J25	1.1e-31	32.58	rRNA methyltransferase 1, mitochondrial OS=Mus musculus OX=10090 GN=Mrm1 PE=2 SV...	[more]
O94631	7.4e-31	34.27	rRNA methyltransferase 1, mitochondrial OS=Schizosaccharomyces pombe (strain 972...	[more]
A4T5L0	8.8e-24	29.80	Uncharacterized tRNA/rRNA methyltransferase Mflv_1447 OS=Mycolicibacterium gilvu...	[more]

BLAST of MS003866 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CVE3	8.2e-283	98.83	uncharacterized protein LOC111014564 OS=Momordica charantia OX=3673 GN=LOC111014...	[more]
A0A0A0LW97	7.3e-223	77.04	SpoU_sub_bind domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G5749...	[more]
A0A5D3CIS9	2.3e-221	77.32	Putative tRNA/rRNA methyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E...	[more]
A0A1S3BQ13	2.3e-221	77.32	uncharacterized tRNA/rRNA methyltransferase MSMEG_6073/MSMEI_5913 OS=Cucumis mel...	[more]
A0A6J1HPM2	9.9e-212	75.00	uncharacterized protein LOC111465489 OS=Cucurbita maxima OX=3661 GN=LOC111465489...	[more]

BLAST of MS003866 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G19870.1	9.3e-130	51.56	tRNA/rRNA methyltransferase (SpoU) family protein	[more]
AT4G38020.1	1.9e-05	27.03	tRNA/rRNA methyltransferase (SpoU) family protein	[more]
AT5G15390.1	2.5e-05	22.53	tRNA/rRNA methyltransferase (SpoU) family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR013123	RNA 2-O ribose methyltransferase, substrate binding	SMART	SM00967	SpoU_sub_bind_2	coord: 236..322 e-value: 8.3E-8 score: 41.9
IPR013123	RNA 2-O ribose methyltransferase, substrate binding	PFAM	PF08032	SpoU_sub_bind	coord: 237..320 e-value: 5.1E-13 score: 49.1
IPR001537	tRNA/rRNA methyltransferase, SpoU type	PFAM	PF00588	SpoU_methylase	coord: 340..463 e-value: 5.6E-26 score: 91.4
IPR029064	50S ribosomal protein L30e-like	GENE3D	3.30.1330.30		coord: 230..318 e-value: 5.8E-17 score: 63.7
IPR029064	50S ribosomal protein L30e-like	SUPERFAMILY	55315	L30e-like	coord: 236..319
IPR029026	tRNA (guanine-N1-)-methyltransferase, N-terminal	GENE3D	3.40.1280.10		coord: 321..520 e-value: 7.5E-48 score: 164.5
IPR004441	RNA methyltransferase TrmH family	TIGRFAM	TIGR00186	TIGR00186	coord: 266..511 e-value: 7.2E-51 score: 170.9
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 64..84
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 85..100
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 149..172
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 149..183
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 56..126
None	No IPR available	PANTHER	PTHR46103	RRNA METHYLTRANSFERASE 1, MITOCHONDRIAL	coord: 45..516
None	No IPR available	CDD	cd18105	SpoU-like_MRM1	coord: 339..512 e-value: 2.01652E-74 score: 230.868
IPR029028	Alpha/beta knot methyltransferases	SUPERFAMILY	75217	alpha/beta knot	coord: 313..463

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS003866.1	MS003866.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006396	RNA processing
molecular_function	GO:0008168	methyltransferase activity
molecular_function	GO:0003723	RNA binding
molecular_function	GO:0008173	RNA methyltransferase activity