MS003801 (gene) Bitter gourd (TR) v1

Overview


Name	MS003801
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	RNase H domain-containing protein
Location	scaffold127: 645631 .. 646167 (+)
RNA-Seq Expression	MS003801
Synteny	MS003801

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

TCCGTTTGGAAGCTTAAAGTTCCACCAAAAGTTAAGATGTTTTTGTGGAGAGTATTCAACGACTGTGTGCCTTGTATGAGAAATCTTATTAAGAGAGGGATTGATCCAAACCCAAATTGTCTAGTATGTGGGAAGCAAATTGAAACAACTGACCATGCTCTTTTTAAATGCAAAAGAGCAAAGGAGGTTTGGAATATTTTGTTACCTCAAGCTTTCCCGCAATGTGATTTTAATAATTCCGTTCAAGATGGATTGTTGTATCTGGTGGAGTCGCTCTCTACTCCAGACTTTGATTTGGTTGGAGTTGGTGTTTGGGCAATCTGGAATGATCGAAATGCAATTAGAATGCAAAGACAAATTCCAGACGCTAAGATTAGGAGCGATTGGATCCTTACCTATGTGAGAGATTTTCAGGTCCGTGATGTCCCTTCTCTCGGTGATTTTCGGATTCAGGAGGATGCAAGTTCGAATGATCGGACTATTGAGAATTACTGGTCGCCTCCTCCGGCTGGTTGGATTAAAATAAACGTGGACGCA

mRNA sequence

TCCGTTTGGAAGCTTAAAGTTCCACCAAAAGTTAAGATGTTTTTGTGGAGAGTATTCAACGACTGTGTGCCTTGTATGAGAAATCTTATTAAGAGAGGGATTGATCCAAACCCAAATTGTCTAGTATGTGGGAAGCAAATTGAAACAACTGACCATGCTCTTTTTAAATGCAAAAGAGCAAAGGAGGTTTGGAATATTTTGTTACCTCAAGCTTTCCCGCAATGTGATTTTAATAATTCCGTTCAAGATGGATTGTTGTATCTGGTGGAGTCGCTCTCTACTCCAGACTTTGATTTGGTTGGAGTTGGTGTTTGGGCAATCTGGAATGATCGAAATGCAATTAGAATGCAAAGACAAATTCCAGACGCTAAGATTAGGAGCGATTGGATCCTTACCTATGTGAGAGATTTTCAGGTCCGTGATGTCCCTTCTCTCGGTGATTTTCGGATTCAGGAGGATGCAAGTTCGAATGATCGGACTATTGAGAATTACTGGTCGCCTCCTCCGGCTGGTTGGATTAAAATAAACGTGGACGCA

Coding sequence (CDS)

TCCGTTTGGAAGCTTAAAGTTCCACCAAAAGTTAAGATGTTTTTGTGGAGAGTATTCAACGACTGTGTGCCTTGTATGAGAAATCTTATTAAGAGAGGGATTGATCCAAACCCAAATTGTCTAGTATGTGGGAAGCAAATTGAAACAACTGACCATGCTCTTTTTAAATGCAAAAGAGCAAAGGAGGTTTGGAATATTTTGTTACCTCAAGCTTTCCCGCAATGTGATTTTAATAATTCCGTTCAAGATGGATTGTTGTATCTGGTGGAGTCGCTCTCTACTCCAGACTTTGATTTGGTTGGAGTTGGTGTTTGGGCAATCTGGAATGATCGAAATGCAATTAGAATGCAAAGACAAATTCCAGACGCTAAGATTAGGAGCGATTGGATCCTTACCTATGTGAGAGATTTTCAGGTCCGTGATGTCCCTTCTCTCGGTGATTTTCGGATTCAGGAGGATGCAAGTTCGAATGATCGGACTATTGAGAATTACTGGTCGCCTCCTCCGGCTGGTTGGATTAAAATAAACGTGGACGCA

Protein sequence

SVWKLKVPPKVKMFLWRVFNDCVPCMRNLIKRGIDPNPNCLVCGKQIETTDHALFKCKRAKEVWNILLPQAFPQCDFNNSVQDGLLYLVESLSTPDFDLVGVGVWAIWNDRNAIRMQRQIPDAKIRSDWILTYVRDFQVRDVPSLGDFRIQEDASSNDRTIENYWSPPPAGWIKINVDA

Homology

[Close]

BLAST of MS003801 vs. NCBI nr
Match: XP_022145060.1 (uncharacterized protein LOC111014578 [Momordica charantia])

HSP 1 Score: 362.8 bits (930), Expect = 1.7e-96
Identity = 166/167 (99.40%), Postives = 166/167 (99.40%), Query Frame = 0


Query: 13  MFLWRVFNDCVPCMRNLIKRGIDPNPNCLVCGKQIETTDHALFKCKRAKEVWNILLPQAF 72
           MFLWRVFNDCVP MRNLIKRGIDPNPNCLVCGKQIETTDHALFKCKRAKEVWNILLPQAF
Sbjct: 1   MFLWRVFNDCVPSMRNLIKRGIDPNPNCLVCGKQIETTDHALFKCKRAKEVWNILLPQAF 60

Query: 73  PQCDFNNSVQDGLLYLVESLSTPDFDLVGVGVWAIWNDRNAIRMQRQIPDAKIRSDWILT 132
           PQCDFNNSVQDGLLYLVESLSTPDFDLVGVGVWAIWNDRNAIRMQRQIPDAKIRSDWILT
Sbjct: 61  PQCDFNNSVQDGLLYLVESLSTPDFDLVGVGVWAIWNDRNAIRMQRQIPDAKIRSDWILT 120

Query: 133 YVRDFQVRDVPSLGDFRIQEDASSNDRTIENYWSPPPAGWIKINVDA 180
           YVRDFQVRDVPSLGDFRIQEDASSNDRTIENYWSPPPAGWIKINVDA
Sbjct: 121 YVRDFQVRDVPSLGDFRIQEDASSNDRTIENYWSPPPAGWIKINVDA 167

[Close]

BLAST of MS003801 vs. NCBI nr
Match: XP_022158377.1 (uncharacterized protein LOC111024874 [Momordica charantia])

HSP 1 Score: 127.9 bits (320), Expect = 8.9e-26
Identity = 60/182 (32.97%), Postives = 95/182 (52.20%), Query Frame = 0


Query: 1    SVWKLKVPPKVKMFLWRVFNDCVPCMRNLIKRGIDPNPNCLVCGKQIETTDHALFKCKRA 60
            S+WKL VP K+K+F+WR  ++ +P  +NL+ RGI   P C +CG + E+  HA F CKRA
Sbjct: 1134 SIWKLTVPTKIKIFIWRSAHEHIPTAQNLLLRGIGELPACTICGDRRESIIHAFFHCKRA 1193

Query: 61   KEVWNILLPQAFPQC---DFNNSVQDGLLYLVESLSTPDFDLVGVGVWAIWNDRNAIRMQ 120
            +++W  L P  F  C   + N S  +    L E L   D +L  +  W IWNDRN++   
Sbjct: 1194 RQIWRTLFP--FLTCLSAEDNISFLELWSSLTEQLEPKDLNLAAITGWGIWNDRNSLIHG 1253

Query: 121  RQIPDAKIRSDWILTYVRDFQVRDVPSLGDFRIQEDASSNDRTIENYWSPPPAGWIKINV 180
            +Q+   + + +W+  ++       + +           SN R +  YW P  +  +K+N 
Sbjct: 1254 KQVSPVEFKCEWLTPFLDSHSQAQMSN-----YSPRTQSNHRPVVQYWRPSSSVSLKLNT 1308

[Close]

BLAST of MS003801 vs. NCBI nr
Match: XP_030509188.1 (uncharacterized protein LOC115723863 [Cannabis sativa])

HSP 1 Score: 122.1 bits (305), Expect = 4.9e-24
Identity = 63/185 (34.05%), Postives = 101/185 (54.59%), Query Frame = 0


Query: 3    WKLKVPPKVKMFLWRVFNDCVPCMRNLIKRGIDPNPNCLVCGKQIETTDHALFKCKRAKE 62
            WK+K+PPKV++F+W+VF+  +P    L +R I  +P+C +C    E+ +HALF C RAK 
Sbjct: 1015 WKMKLPPKVRIFVWKVFHSTLPVAAELFRRHIATSPHCSICNSAEESINHALFDCPRAKA 1074

Query: 63   VWNILLPQAFPQCDFNNSVQ----DGLLYLVESLSTPDFDLVGVGVWAIWNDRNAIRMQR 122
            VW +    +    DF+   Q    D LL+L  +LST +F+L  V  W  W++RNAI    
Sbjct: 1075 VWEL----SSLHIDFHTLRQSASADILLHLSTALSTSEFELFLVLCWCNWHERNAIYHGN 1134

Query: 123  QIPDAKIRSDWILTYVRDFQ----VRDVPSLGDFRIQEDASSNDRTIENYWSPPPAGWIK 180
             +  ++  + +  +Y+ +FQ     R  P+       +   S++ T    W+ PP G +K
Sbjct: 1135 TVRSSQAVASYAPSYLAEFQNARAKRSQPTPISIAATDPRPSSEFTHAPKWTAPPQGRLK 1194

[Close]

BLAST of MS003801 vs. NCBI nr
Match: XP_030498020.1 (uncharacterized protein LOC115713676 [Cannabis sativa])

HSP 1 Score: 116.7 bits (291), Expect = 2.1e-22
Identity = 52/168 (30.95%), Postives = 89/168 (52.98%), Query Frame = 0


Query: 1   SVWKLKVPPKVKMFLWRVFNDCVPCMRNLIKRGIDPNPNCLVCGKQIETTDHALFKCKRA 60
           ++W L VPPK+K F+W+ F+  +PC  NL  +   P+PNC +CG   E+  HAL  C RA
Sbjct: 731 NLWSLSVPPKIKHFIWKAFHHILPCAFNLFLKRSLPHPNCSICGNTTESLTHALIGCPRA 790

Query: 61  KEVWNILLPQAFPQCDFNNSVQDGLLYLVESLSTPDFDLVGVGVWAIWNDRNAIRMQRQI 120
           K +W     + F      + ++D L+  + ++S  DF +    VW IWN RN+I   +  
Sbjct: 791 KSIWKSSRFKQFYLAYHRSDIKDFLIQALLNISKQDFPIFIAFVWQIWNSRNSILFNKSS 850

Query: 121 PDAKIRSDWILTYVRDFQVRDVPSLGDFRIQEDASSNDRTIENYWSPP 169
               +  ++++ Y++D+        GD  +  +A+   RT +++  PP
Sbjct: 851 VPNNV-EEFVVNYLQDYSAAQCNYQGD-NLDSEAAPIPRTSQHHHPPP 896

[Close]

BLAST of MS003801 vs. NCBI nr
Match: XP_030508852.1 (uncharacterized protein LOC115723496 [Cannabis sativa])

HSP 1 Score: 116.3 bits (290), Expect = 2.7e-22
Identity = 62/181 (34.25%), Postives = 91/181 (50.28%), Query Frame = 0


Query: 3    WKLKVPPKVKMFLWRVFNDCVPCMRNLIKRGIDPNPNCLVCGKQIETTDHALFKCKRAKE 62
            WKLK+PPKV++F+W+VF+  +P    L +R I  +P C +C    ET  HALF C RAK 
Sbjct: 1056 WKLKLPPKVRIFVWKVFHTSLPVAAELYRRHIAFSPYCTICNSCEETVHHALFSCPRAKA 1115

Query: 63   VWNILLPQAFPQCDFNNSVQDGLLYLVESLSTPDFDLVGVGVWAIWNDRNAIRMQRQIPD 122
            VW +       Q    +S  D LL L  SLS+ + +L  V  W+IW++RNAI     +  
Sbjct: 1116 VWELSNFSIDFQTIERSSTADTLLLLSTSLSSSELELFLVLCWSIWHERNAIYHGNSVRT 1175

Query: 123  AKIRSDWILTYVRDFQVRDV----PSLGDFRIQEDASSNDRTIENYWSPPPAGWIKINVD 180
                + +  +Y+ +FQ        P            S++      W+ PP G +K+N D
Sbjct: 1176 PAAVAAYAPSYLTEFQQARAKNAKPVTASGAATPSRPSSEFIHAPKWTTPPRGRLKLNTD 1235

[Close]

BLAST of MS003801 vs. ExPASy Swiss-Prot
Match: P0C2F6 (Putative ribonuclease H protein At1g65750 OS=Arabidopsis thaliana OX=3702 GN=At1g65750 PE=3 SV=1)

HSP 1 Score: 64.7 bits (156), Expect = 1.2e-09
Identity = 46/184 (25.00%), Postives = 73/184 (39.67%), Query Frame = 0


Query: 2   VWKLKVPPKVKMFLWRVFNDCVPCMRNLIKRGIDPNPNCLVCGKQIETTDHALFKCKRAK 61
           +WK++VP +VK FLW V N  V       +R +  +  C VC   +E+  H L  C    
Sbjct: 291 LWKVRVPERVKTFLWLVGNQAVMTEEERHRRHLSASNVCQVCKGGVESMLHVLRDCPAQL 350

Query: 62  EVWNILLPQAFPQCDFNNSVQDGLLYLVESLS----TPDFDLVGVGVWAIWNDR--NAIR 121
            +W  ++PQ   Q  F+ S+ + L   +   S     P   +  V +W  W  R  N   
Sbjct: 351 GIWVRVVPQRRQQGFFSKSLFEWLYDNLGDRSGCEDIPWSTIFAVIIWWGWKWRCGNIFG 410

Query: 122 MQRQIPD-AKIRSDWILTYVRDFQVRDVPSLGDFRIQEDASSNDRTIENYWSPPPAGWIK 179
              +  D  K   +W +   R      +  +   R++             W  P  GW+K
Sbjct: 411 ENTKCRDRVKFVKEWAVEVYRAHSGNVLVGITQPRVERMIG---------WVSPCVGWVK 465

[Close]

BLAST of MS003801 vs. ExPASy TrEMBL
Match: A0A6J1CTE3 (uncharacterized protein LOC111014578 OS=Momordica charantia OX=3673 GN=LOC111014578 PE=4 SV=1)

HSP 1 Score: 362.8 bits (930), Expect = 8.0e-97
Identity = 166/167 (99.40%), Postives = 166/167 (99.40%), Query Frame = 0


Query: 13  MFLWRVFNDCVPCMRNLIKRGIDPNPNCLVCGKQIETTDHALFKCKRAKEVWNILLPQAF 72
           MFLWRVFNDCVP MRNLIKRGIDPNPNCLVCGKQIETTDHALFKCKRAKEVWNILLPQAF
Sbjct: 1   MFLWRVFNDCVPSMRNLIKRGIDPNPNCLVCGKQIETTDHALFKCKRAKEVWNILLPQAF 60

Query: 73  PQCDFNNSVQDGLLYLVESLSTPDFDLVGVGVWAIWNDRNAIRMQRQIPDAKIRSDWILT 132
           PQCDFNNSVQDGLLYLVESLSTPDFDLVGVGVWAIWNDRNAIRMQRQIPDAKIRSDWILT
Sbjct: 61  PQCDFNNSVQDGLLYLVESLSTPDFDLVGVGVWAIWNDRNAIRMQRQIPDAKIRSDWILT 120

Query: 133 YVRDFQVRDVPSLGDFRIQEDASSNDRTIENYWSPPPAGWIKINVDA 180
           YVRDFQVRDVPSLGDFRIQEDASSNDRTIENYWSPPPAGWIKINVDA
Sbjct: 121 YVRDFQVRDVPSLGDFRIQEDASSNDRTIENYWSPPPAGWIKINVDA 167

[Close]

BLAST of MS003801 vs. ExPASy TrEMBL
Match: A0A6J1DX30 (uncharacterized protein LOC111024874 OS=Momordica charantia OX=3673 GN=LOC111024874 PE=4 SV=1)

HSP 1 Score: 127.9 bits (320), Expect = 4.3e-26
Identity = 60/182 (32.97%), Postives = 95/182 (52.20%), Query Frame = 0


Query: 1    SVWKLKVPPKVKMFLWRVFNDCVPCMRNLIKRGIDPNPNCLVCGKQIETTDHALFKCKRA 60
            S+WKL VP K+K+F+WR  ++ +P  +NL+ RGI   P C +CG + E+  HA F CKRA
Sbjct: 1134 SIWKLTVPTKIKIFIWRSAHEHIPTAQNLLLRGIGELPACTICGDRRESIIHAFFHCKRA 1193

Query: 61   KEVWNILLPQAFPQC---DFNNSVQDGLLYLVESLSTPDFDLVGVGVWAIWNDRNAIRMQ 120
            +++W  L P  F  C   + N S  +    L E L   D +L  +  W IWNDRN++   
Sbjct: 1194 RQIWRTLFP--FLTCLSAEDNISFLELWSSLTEQLEPKDLNLAAITGWGIWNDRNSLIHG 1253

Query: 121  RQIPDAKIRSDWILTYVRDFQVRDVPSLGDFRIQEDASSNDRTIENYWSPPPAGWIKINV 180
            +Q+   + + +W+  ++       + +           SN R +  YW P  +  +K+N 
Sbjct: 1254 KQVSPVEFKCEWLTPFLDSHSQAQMSN-----YSPRTQSNHRPVVQYWRPSSSVSLKLNT 1308

[Close]

BLAST of MS003801 vs. ExPASy TrEMBL
Match: A0A803Q2K8 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 122.1 bits (305), Expect = 2.4e-24
Identity = 63/185 (34.05%), Postives = 101/185 (54.59%), Query Frame = 0


Query: 3    WKLKVPPKVKMFLWRVFNDCVPCMRNLIKRGIDPNPNCLVCGKQIETTDHALFKCKRAKE 62
            WK+K+PPKV++F+W+VF+  +P    L +R I  +P+C +C    E+ +HALF C RAK 
Sbjct: 1414 WKMKLPPKVRIFVWKVFHSTLPVAAELFRRHIATSPHCSICNSAEESINHALFDCPRAKA 1473

Query: 63   VWNILLPQAFPQCDFNNSVQ----DGLLYLVESLSTPDFDLVGVGVWAIWNDRNAIRMQR 122
            VW +    +    DF+   Q    D LL+L  +LST +F+L  V  W  W++RNAI    
Sbjct: 1474 VWEL----SSLHIDFHTLRQSASADILLHLSTALSTSEFELFLVLCWCNWHERNAIYHGN 1533

Query: 123  QIPDAKIRSDWILTYVRDFQ----VRDVPSLGDFRIQEDASSNDRTIENYWSPPPAGWIK 180
             +  ++  + +  +Y+ +FQ     R  P+       +   S++ T    W+ PP G +K
Sbjct: 1534 TVRSSQAVASYAPSYLAEFQNARAKRSQPTPISIAATDPRPSSEFTHAPKWTAPPQGRLK 1593

[Close]

BLAST of MS003801 vs. ExPASy TrEMBL
Match: A0A803PM52 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 114.4 bits (285), Expect = 5.0e-22
Identity = 62/193 (32.12%), Postives = 98/193 (50.78%), Query Frame = 0


Query: 2    VWKLKVPPKVKMFLWRVFNDCVPCMRNLIKRGIDPNPNCLVCGKQIETTDHALFKCKRAK 61
            +W LK+P K+K+F+WR FN+ +P    L+K+ +  +  C +C +  E+  HALF+CK A+
Sbjct: 1203 LWSLKLPEKIKIFVWRTFNEALPVATALVKQKVISDSTCSICHQAWESIGHALFRCKYAR 1262

Query: 62   EVW---NILLPQAFPQCDFNNSVQ----DGLLYLVESLSTPDFDLVGVGVWAIWNDRNAI 121
             VW   N LL       D+NNS      D L++L    +  + + +   +W+IW +RN +
Sbjct: 1263 AVWRHSNFLL-------DWNNSSTMCKGDYLVHLSSIYTKVEMEQILCIMWSIWTERNRV 1322

Query: 122  RMQRQIPDAKIRSDWILTYVRDFQVRDVPSLGDFRIQEDAS--------SNDRTIENYWS 180
                +   AK+ S + L Y ++F +     L D   Q   S         +  TI   W 
Sbjct: 1323 VHGNRAKSAKLLSSFALNYWQNFSIAQQRHLSDHPPQNSGSPTAAAVVQPSATTIPLPWK 1382

[Close]

BLAST of MS003801 vs. ExPASy TrEMBL
Match: A0A803PH33 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 112.5 bits (280), Expect = 1.9e-21
Identity = 62/182 (34.07%), Postives = 94/182 (51.65%), Query Frame = 0


Query: 3   WKLKVPPKVKMFLWRVFNDCVPCMRNLIKRGIDPNPNCLVCGKQIETTDHALFKCKRAKE 62
           WKL++P K+++F+W+VF++ +P    L +R I  +P C +C  Q ET  HALF C RAKE
Sbjct: 263 WKLQLPSKLRIFVWKVFHNVLPVASELNRRHIAASPYCPLCKLQQETIPHALFLCSRAKE 322

Query: 63  VWNILLPQAFPQCDFNNSVQDGLLYLVESLSTPDFDLVGVGVWAIWNDRNAIRMQRQIPD 122
           VW I       +   ++S ++ LLY+ E  ST +F+      W+IW +RNA    +    
Sbjct: 323 VWRISGLNLNFKLAASSSAEEFLLYVSEHTSTLEFEQFLTLCWSIWFERNAEFHDKPAKC 382

Query: 123 AKIRSDWILTYVRDFQ-VRDVPSLGDFRIQEDASSNDRTIE----NYWSPPPAGWIKINV 180
                D+ L Y+  +Q V D P++    +   A     T      + W  PP G  K+N 
Sbjct: 383 PTAIKDFALQYITKYQSVHDKPNVPSPDVAPAAPFPTSTFAAPSVDPWKAPPPGTYKLNT 442

[Close]

BLAST of MS003801 vs. TAIR 10
Match: AT2G02650.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 92.8 bits (229), Expect = 3.0e-19
Identity = 54/187 (28.88%), Postives = 85/187 (45.45%), Query Frame = 0


Query: 1   SVWKLKVPPKVKMFLWRVFNDCVPCMRNLIKRGIDPNPNCLVCGKQIETTDHALFKCKRA 60
           ++WKL V PK+K FLWR     +     L  R ID +P C  C  + ET  H +F C   
Sbjct: 36  AIWKLHVAPKIKHFLWRCVTGALATNTRLRSRNIDADPICQRCCIEEETIHHIMFNCPYT 95

Query: 61  KEVW---NILLPQAF-PQCDFNNSVQDGLLYLVESLSTPDFD--LVGVGVWAIWNDRNAI 120
           + VW   NI++   + P   F +++ + L+ L ++ +T   D  L    +W +W  RN  
Sbjct: 96  QSVWRSANIIIGNQWGPPSSFEDNL-NRLIQLSKTQTTNSLDRFLPFWIMWRLWKSRNVF 155

Query: 121 RMQR--QIPDAKIRSDWILTYVRDFQVRDVPSLGDFRIQEDASSNDRTIENYWSPPPAGW 180
             Q+  Q PD + R   I          +     +  +  +     R   + W+PPP GW
Sbjct: 156 LFQQKCQSPDYEARKG-IQDATEWLNANETTENTNVHVATNPIQTSRRDSSQWNPPPEGW 215

[Close]

BLAST of MS003801 vs. TAIR 10
Match: AT3G09510.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 79.0 bits (193), Expect = 4.4e-15
Identity = 52/188 (27.66%), Postives = 84/188 (44.68%), Query Frame = 0


Query: 2   VWKLKVPPKVKMFLWRVFNDCVPCMRNLIKRGIDPNPNCLVCGKQIETTDHALFKCKRAK 61
           +W L + PK+K FLWR  +  +     L  RG+  +P+C  C ++ E+ +HALF C  A 
Sbjct: 162 IWNLPIMPKLKHFLWRALSQALATTERLTTRGMRIDPSCPRCHRENESINHALFTCPFAT 221

Query: 62  EVWNI----LLPQAFPQCDFNNSVQDGLLYLVESLSTPDFDLVGVG-VWAIWNDRNAIRM 121
             W +    L+       DF  ++ + L ++ ++  +    L+ V  +W IW  RN +  
Sbjct: 222 MAWRLSDSSLIRNQLMSNDFEENISNILNFVQDTTMSDFHKLLPVWLIWRIWKARNNVVF 281

Query: 122 Q--RQIPDAKIRSDWILTYVRDFQVRDVPSLGDFRIQEDASSNDRTI-EN--YWSPPPAG 180
              R+ P   + S    T+           L   +  +   S  R I EN   W  PPA 
Sbjct: 282 NKFRESPSKTVLSAKAETH---------DWLNATQSHKKTPSPTRQIAENKIEWRNPPAT 340

[Close]

BLAST of MS003801 vs. TAIR 10
Match: AT3G25270.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 75.5 bits (184), Expect = 4.9e-14
Identity = 47/183 (25.68%), Postives = 78/183 (42.62%), Query Frame = 0


Query: 2   VWKLKVPPKVKMFLWRVFNDCVPCMRNLIKRGIDPNPNCLVCGKQIETTDHALFKCKRAK 61
           +WKLK  PK+K FLW++ +  +    NL +R I  +P C  C ++ ET+ H  F C  A+
Sbjct: 18  IWKLKTAPKIKHFLWKLLSGALATGDNLKRRHIRNHPQCHRCCQEDETSQHLFFDCFYAQ 77

Query: 62  EVWNIL-LPQAFPQCDFNNSVQDGLLYLVESLSTPDFDLVGVGV---WAIWNDRNAIRMQ 121
           +VW    +P    +           L L   L+     L  + +   W +W  RN +  Q
Sbjct: 78  QVWRASGIPHQELRTTGITMETKMELLLSSCLANRQPQLFNLAIWILWRLWKSRNQLVFQ 137

Query: 122 RQIPDAKIRSDWILTYVRDFQVRD--VPSLGDFRIQEDASSNDRTIENYWSPPPAGWIKI 179
           ++    +         V++++  +  V SL   ++              W  PP+ WIK 
Sbjct: 138 QKSISWQNTLQRARNDVQEWEDTNTYVQSLNQ-QVHSSRHQQPTMARTKWQRPPSTWIKY 197

[Close]

BLAST of MS003801 vs. TAIR 10
Match: AT3G26855.1 (RNA-directed DNA polymerase (reverse transcriptase)-related family protein )

HSP 1 Score: 55.1 bits (131), Expect = 6.9e-08
Identity = 22/60 (36.67%), Postives = 33/60 (55.00%), Query Frame = 0


Query: 2  VWKLKVPPKVKMFLWRVFNDCVPCMRNLIKRGIDPNPNCLVCGKQIETTDHALFKCKRAK 61
          +W LK+ PK+K+ +W+  N+ +P    L+ R I   P C  C +  ET  H LF C  A+
Sbjct: 9  IWSLKISPKIKLLIWKALNNALPVGAQLLSRNISIEPFCTRC-RDFETITHILFNCPFAQ 67

The following BLAST results are available for this feature:

BLAST of MS003801 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022145060.1	1.7e-96	99.40	uncharacterized protein LOC111014578 [Momordica charantia]	[more]
XP_022158377.1	8.9e-26	32.97	uncharacterized protein LOC111024874 [Momordica charantia]	[more]
XP_030509188.1	4.9e-24	34.05	uncharacterized protein LOC115723863 [Cannabis sativa]	[more]
XP_030498020.1	2.1e-22	30.95	uncharacterized protein LOC115713676 [Cannabis sativa]	[more]
XP_030508852.1	2.7e-22	34.25	uncharacterized protein LOC115723496 [Cannabis sativa]	[more]

BLAST of MS003801 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P0C2F6	1.2e-09	25.00	Putative ribonuclease H protein At1g65750 OS=Arabidopsis thaliana OX=3702 GN=At1...	[more]

BLAST of MS003801 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CTE3	8.0e-97	99.40	uncharacterized protein LOC111014578 OS=Momordica charantia OX=3673 GN=LOC111014...	[more]
A0A6J1DX30	4.3e-26	32.97	uncharacterized protein LOC111024874 OS=Momordica charantia OX=3673 GN=LOC111024...	[more]
A0A803Q2K8	2.4e-24	34.05	Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1	[more]
A0A803PM52	5.0e-22	32.12	Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1	[more]
A0A803PH33	1.9e-21	34.07	Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1	[more]

BLAST of MS003801 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G02650.1	3.0e-19	28.88	Ribonuclease H-like superfamily protein	[more]
AT3G09510.1	4.4e-15	27.66	Ribonuclease H-like superfamily protein	[more]
AT3G25270.1	4.9e-14	25.68	Ribonuclease H-like superfamily protein	[more]
AT3G26855.1	6.9e-08	36.67	RNA-directed DNA polymerase (reverse transcriptase)-related family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR026960	Reverse transcriptase zinc-binding domain	PFAM	PF13966	zf-RVT	coord: 2..64 e-value: 2.0E-19 score: 70.1
None	No IPR available	PANTHER	PTHR46736:SF6	SUBFAMILY NOT NAMED	coord: 2..179
None	No IPR available	PANTHER	PTHR46736	FAMILY NOT NAMED	coord: 2..179

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS003801.1	MS003801.1	mRNA