MS003445 (gene) Bitter gourd (TR) v1

Overview


Name	MS003445
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Cytoplasmic tRNA 2-thiolation protein 1
Location	scaffold234: 2875207 .. 2875488 (+)
RNA-Seq Expression	MS003445
Synteny	MS003445

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ACATTATTATATATCAACATTCAAGAATATAATCAACAAAGAACAAACATGGAGAAGCAAGAGAAGATCGAGATGAAACCAAGCTTCTTTCAAATGCCCCTTCACTATCCTAGATACTCGAAGAAGGACTACGAGGACATGCCTGAATGGAAGCTCGACCGCCTCCTCGCCGACTACGGCCTCCCGACCTCGGGTGACTTGCCCTTCAAACGCCGCTTCGCCATGGGTGCATTCCTCTGGCCTGAGTTCCACCATCCCTCTCTTCTCAACAAAAAACTCATC

mRNA sequence

ACATTATTATATATCAACATTCAAGAATATAATCAACAAAGAACAAACATGGAGAAGCAAGAGAAGATCGAGATGAAACCAAGCTTCTTTCAAATGCCCCTTCACTATCCTAGATACTCGAAGAAGGACTACGAGGACATGCCTGAATGGAAGCTCGACCGCCTCCTCGCCGACTACGGCCTCCCGACCTCGGGTGACTTGCCCTTCAAACGCCGCTTCGCCATGGGTGCATTCCTCTGGCCTGAGTTCCACCATCCCTCTCTTCTCAACAAAAAACTCATC

Coding sequence (CDS)

ACATTATTATATATCAACATTCAAGAATATAATCAACAAAGAACAAACATGGAGAAGCAAGAGAAGATCGAGATGAAACCAAGCTTCTTTCAAATGCCCCTTCACTATCCTAGATACTCGAAGAAGGACTACGAGGACATGCCTGAATGGAAGCTCGACCGCCTCCTCGCCGACTACGGCCTCCCGACCTCGGGTGACTTGCCCTTCAAACGCCGCTTCGCCATGGGTGCATTCCTCTGGCCTGAGTTCCACCATCCCTCTCTTCTCAACAAAAAACTCATC

Protein sequence

TLLYINIQEYNQQRTNMEKQEKIEMKPSFFQMPLHYPRYSKKDYEDMPEWKLDRLLADYGLPTSGDLPFKRRFAMGAFLWPEFHHPSLLNKKLI

Homology

[Close]

BLAST of MS003445 vs. NCBI nr
Match: KAG6578420.1 (hypothetical protein SDJN03_22868, partial [Cucurbita argyrosperma subsp. sororia] >KAG7015984.1 hypothetical protein SDJN02_21088, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 120.2 bits (300), Expect = 9.8e-24
Identity = 56/83 (67.47%), Postives = 66/83 (79.52%), Query Frame = 0


Query: 17 MEKQEKIEMKPSFFQMPLHYPRYSKKDYEDMPEWKLDRLLADYGLPTSGDLPFKRRFAMG 76
          ME  EK +   +FFQMPLHYPRYSK DYE+MPEWKLDRLL +YGLPT G+LP+KR+FAMG
Sbjct: 1  MENNEKKQGGDNFFQMPLHYPRYSKMDYENMPEWKLDRLLLEYGLPTHGNLPYKRQFAMG 60

Query: 77 AFLWPEFH-----HPSLLNKKLI 95
          AFLW ++H      PSLLN KL+
Sbjct: 61 AFLWHDYHPTFKLDPSLLNNKLM 83

[Close]

BLAST of MS003445 vs. NCBI nr
Match: XP_040869322.1 (uncharacterized protein LOC121174311, partial [Glycine max])

HSP 1 Score: 117.5 bits (293), Expect = 6.3e-23
Identity = 50/66 (75.76%), Postives = 56/66 (84.85%), Query Frame = 0


Query: 29 FFQMPLHYPRYSKKDYEDMPEWKLDRLLADYGLPTSGDLPFKRRFAMGAFLWPEFHHPSL 88
          FFQMPLHYPRY++KDY+DMPEWKLDRLL +YGLPT GDL +KR FAMGAFLWP   H  L
Sbjct: 12 FFQMPLHYPRYTQKDYQDMPEWKLDRLLKEYGLPTHGDLAYKREFAMGAFLWPNIKHYKL 71

Query: 89 LNKKLI 95
           NKK+I
Sbjct: 72 YNKKII 77

[Close]

BLAST of MS003445 vs. NCBI nr
Match: KAF3433650.1 (hypothetical protein FNV43_RR24753 [Rhamnella rubrinervis])

HSP 1 Score: 111.3 bits (277), Expect = 4.5e-21
Identity = 48/63 (76.19%), Postives = 54/63 (85.71%), Query Frame = 0


Query: 28 SFFQMPLHYPRYSKKDYEDMPEWKLDRLLADYGLPTSGDLPFKRRFAMGAFLWPEFHHPS 87
          SFFQMPLHYPRY++KDY+DMPEWKLDRLLADYGL   GDL +KR FAMGAFLWP FH  +
Sbjct: 10 SFFQMPLHYPRYTRKDYQDMPEWKLDRLLADYGLSIQGDLTYKRDFAMGAFLWPGFHQQA 69

Query: 88 LLN 91
           L+
Sbjct: 70 SLS 72

[Close]

BLAST of MS003445 vs. NCBI nr
Match: XP_010108400.1 (uncharacterized protein LOC21393700 [Morus notabilis] >EXC19391.1 hypothetical protein L484_010408 [Morus notabilis])

HSP 1 Score: 111.3 bits (277), Expect = 4.5e-21
Identity = 48/68 (70.59%), Postives = 58/68 (85.29%), Query Frame = 0


Query: 20 QEKIEMKPSFFQMPLHYPRYSKKDYEDMPEWKLDRLLADYGLPTSGDLPFKRRFAMGAFL 79
          ++K E   S+FQMPLHYPRY+K+DY+DMPEWKLD+LLA YGLPT GDL +KR FA+GAFL
Sbjct: 5  KKKNESGVSYFQMPLHYPRYTKQDYQDMPEWKLDQLLAQYGLPTHGDLAYKRDFAIGAFL 64

Query: 80 WPEFHHPS 88
          WP+FH  S
Sbjct: 65 WPDFHQGS 72

[Close]

BLAST of MS003445 vs. NCBI nr
Match: OVA17787.1 (hypothetical protein BVC80_1835g174 [Macleaya cordata])

HSP 1 Score: 110.9 bits (276), Expect = 5.9e-21
Identity = 48/70 (68.57%), Postives = 57/70 (81.43%), Query Frame = 0


Query: 17 MEKQEKIEMKPSFFQMPLHYPRYSKKDYEDMPEWKLDRLLADYGLPTSGDLPFKRRFAMG 76
          ME ++K  +  S+FQMPLHYPRYS+KDYEDMPEWKLDRLL +YGLP  GDL +KR +AMG
Sbjct: 1  MENKKKNGVSSSYFQMPLHYPRYSRKDYEDMPEWKLDRLLMEYGLPAMGDLAYKREYAMG 60

Query: 77 AFLWPEFHHP 87
          AFLWP+   P
Sbjct: 61 AFLWPDIVPP 70

[Close]

BLAST of MS003445 vs. ExPASy TrEMBL
Match: W9SNS4 (Uncharacterized protein OS=Morus notabilis OX=981085 GN=L484_010408 PE=4 SV=1)

HSP 1 Score: 111.3 bits (277), Expect = 2.2e-21
Identity = 48/68 (70.59%), Postives = 58/68 (85.29%), Query Frame = 0


Query: 20 QEKIEMKPSFFQMPLHYPRYSKKDYEDMPEWKLDRLLADYGLPTSGDLPFKRRFAMGAFL 79
          ++K E   S+FQMPLHYPRY+K+DY+DMPEWKLD+LLA YGLPT GDL +KR FA+GAFL
Sbjct: 5  KKKNESGVSYFQMPLHYPRYTKQDYQDMPEWKLDQLLAQYGLPTHGDLAYKRDFAIGAFL 64

Query: 80 WPEFHHPS 88
          WP+FH  S
Sbjct: 65 WPDFHQGS 72

[Close]

BLAST of MS003445 vs. ExPASy TrEMBL
Match: A0A061FTQ6 (Cytoplasmic tRNA 2-thiolation protein 1 OS=Theobroma cacao OX=3641 GN=TCM_042740 PE=4 SV=1)

HSP 1 Score: 110.9 bits (276), Expect = 2.9e-21
Identity = 49/67 (73.13%), Postives = 57/67 (85.07%), Query Frame = 0


Query: 18 EKQEKIEMKPSFFQMPLHYPRYSKKDYEDMPEWKLDRLLADYGLPTSGDLPFKRRFAMGA 77
          EKQ  +    SFFQMPLHYPRY++KDY+DMPEWKLDRLLA+YGL   GDL +KR+FAMGA
Sbjct: 4  EKQNGV----SFFQMPLHYPRYTQKDYQDMPEWKLDRLLAEYGLSNKGDLAYKRQFAMGA 63

Query: 78 FLWPEFH 85
          FLWP+FH
Sbjct: 64 FLWPDFH 66

[Close]

BLAST of MS003445 vs. ExPASy TrEMBL
Match: A0A200R4Z5 (Uncharacterized protein OS=Macleaya cordata OX=56857 GN=BVC80_1835g174 PE=4 SV=1)

HSP 1 Score: 110.9 bits (276), Expect = 2.9e-21
Identity = 48/70 (68.57%), Postives = 57/70 (81.43%), Query Frame = 0


Query: 17 MEKQEKIEMKPSFFQMPLHYPRYSKKDYEDMPEWKLDRLLADYGLPTSGDLPFKRRFAMG 76
          ME ++K  +  S+FQMPLHYPRYS+KDYEDMPEWKLDRLL +YGLP  GDL +KR +AMG
Sbjct: 1  MENKKKNGVSSSYFQMPLHYPRYSRKDYEDMPEWKLDRLLMEYGLPAMGDLAYKREYAMG 60

Query: 77 AFLWPEFHHP 87
          AFLWP+   P
Sbjct: 61 AFLWPDIVPP 70

[Close]

BLAST of MS003445 vs. ExPASy TrEMBL
Match: A0A2P5ALC9 (Uncharacterized protein OS=Parasponia andersonii OX=3476 GN=PanWU01x14_320780 PE=4 SV=1)

HSP 1 Score: 110.2 bits (274), Expect = 4.9e-21
Identity = 50/69 (72.46%), Postives = 59/69 (85.51%), Query Frame = 0


Query: 28 SFFQMPLHYPRYSKKDYEDMPEWKLDRLLADYGLPTSGDLPFKRRFAMGAFLWPE---FH 87
          S+FQMPLHYPRY+KKDY+DMPEWKLDRLLA+YGL T GDL +KR FA+GAFLWP+   F 
Sbjct: 31 SYFQMPLHYPRYTKKDYQDMPEWKLDRLLAEYGLSTHGDLAYKRDFAIGAFLWPDYRTFT 90

Query: 88 HPSLLNKKL 94
            SLL+KK+
Sbjct: 91 DASLLDKKV 99

[Close]

BLAST of MS003445 vs. ExPASy TrEMBL
Match: A0A1R3I234 (Uncharacterized protein OS=Corchorus olitorius OX=93759 GN=COLO4_25500 PE=4 SV=1)

HSP 1 Score: 110.2 bits (274), Expect = 4.9e-21
Identity = 46/57 (80.70%), Postives = 53/57 (92.98%), Query Frame = 0


Query: 28 SFFQMPLHYPRYSKKDYEDMPEWKLDRLLADYGLPTSGDLPFKRRFAMGAFLWPEFH 85
          SFFQMPLHYPRY+KKDY++M EWK+DRLLADYGL T GDL +KR+FAMGAFLWP+FH
Sbjct: 11 SFFQMPLHYPRYTKKDYQEMAEWKVDRLLADYGLSTKGDLDYKRQFAMGAFLWPDFH 67

[Close]

BLAST of MS003445 vs. TAIR 10
Match: AT3G55570.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G41761.1); Has 128 Blast hits to 128 proteins in 12 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 128; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 102.4 bits (254), Expect = 2.0e-22
Identity = 46/67 (68.66%), Postives = 53/67 (79.10%), Query Frame = 0


Query: 28 SFFQMPLHYPRYSKKDYEDMPEWKLDRLLADYGLPTSGDLPFKRRFAMGAFLWPEFHHPS 87
          S F+MPLHYPRYSK+DY+DMPEWKLDR+LADYGL T GDL  KR FA+GAFLW    +P 
Sbjct: 30 SVFRMPLHYPRYSKEDYQDMPEWKLDRVLADYGLSTYGDLAHKRDFAIGAFLWISTRNPK 89

Query: 88 LLNKKLI 95
          L  +K I
Sbjct: 90 LYQEKSI 96

[Close]

BLAST of MS003445 vs. TAIR 10
Match: AT5G41761.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: cellular_component unknown; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT3G55570.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 87.8 bits (216), Expect = 5.0e-18
Identity = 38/60 (63.33%), Postives = 48/60 (80.00%), Query Frame = 0


Query: 21 EKIEMKPSFFQMPLHYPRYSKKDYEDMPEWKLDRLLADYGLPTSGDLPFKRRFAMGAFLW 80
          +K + + S FQ+PLHYP+Y+K DYE MPEW+LDRLL +YGLP  GD   KR+FA+GAFLW
Sbjct: 36 DKPQNQSSSFQIPLHYPKYTKSDYEKMPEWQLDRLLREYGLPVIGDSYEKRKFAIGAFLW 95

[Close]

BLAST of MS003445 vs. TAIR 10
Match: AT3G09950.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G41761.1); Has 128 Blast hits to 128 proteins in 12 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 128; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 80.1 bits (196), Expect = 1.0e-15
Identity = 40/87 (45.98%), Postives = 57/87 (65.52%), Query Frame = 0


Query: 2  LLYINIQEYNQQRTNMEKQEKIEMKPSFFQMPLHYPRYSKKDYEDMPEWKLDRLLADYGL 61
          L ++N  +  +   N++K   ++   S F+MPLHYPRY+K+DYE+M EW+LD LL++YGL
Sbjct: 8  LNFLNHAKNQESVENLDKNGAVKAPSSGFKMPLHYPRYTKEDYEEMEEWRLDLLLSEYGL 67

Query: 62 PTSGD--LPFKRRFAMGAFLWPEFHHP 87
              D  L  KR FA+  F+WP  HHP
Sbjct: 68 LAFHDNTLHEKRAFAIDTFIWP--HHP 92

[Close]

BLAST of MS003445 vs. TAIR 10
Match: AT5G55620.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT3G09950.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 72.8 bits (177), Expect = 1.7e-13
Identity = 36/69 (52.17%), Postives = 44/69 (63.77%), Query Frame = 0


Query: 14 RTNMEKQEKIEMKPSFFQMPLHYPRYSKKDYEDMPEWKLDRLLADYGLPTSGDLPFKRRF 73
          R  + K  K E  PS FQ+PLHYP+YSK DYE M + +LD LL  YG    G L  KR F
Sbjct: 31 RNKIIKMMKKEEFPSGFQVPLHYPKYSKSDYEVMDDLRLDLLLKQYGFSFEGSLEDKRVF 90

Query: 74 AMGAFLWPE 83
          A+ +FLWP+
Sbjct: 91 AIESFLWPD 99

[Close]

BLAST of MS003445 vs. TAIR 10
Match: AT3G11405.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT3G55570.1); Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink). )

HSP 1 Score: 70.1 bits (170), Expect = 1.1e-12
Identity = 34/55 (61.82%), Postives = 41/55 (74.55%), Query Frame = 0


Query: 27  PSFFQMPLHYPRYSKKDYEDMPEWKLDRLLADYGLPTS-GDLPFKRRFAMGAFLW 81
           PS FQMPL YP Y+K+ Y+ M E +LDRLL  YGLPT  G+L  K+ FA+GAFLW
Sbjct: 54  PSSFQMPLQYPNYAKEQYDIMSEEELDRLLKLYGLPTDIGNLSCKKEFAVGAFLW 108

The following BLAST results are available for this feature:

BLAST of MS003445 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6578420.1	9.8e-24	67.47	hypothetical protein SDJN03_22868, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_040869322.1	6.3e-23	75.76	uncharacterized protein LOC121174311, partial [Glycine max]	[more]
KAF3433650.1	4.5e-21	76.19	hypothetical protein FNV43_RR24753 [Rhamnella rubrinervis]	[more]
XP_010108400.1	4.5e-21	70.59	uncharacterized protein LOC21393700 [Morus notabilis] >EXC19391.1 hypothetical p...	[more]
OVA17787.1	5.9e-21	68.57	hypothetical protein BVC80_1835g174 [Macleaya cordata]	[more]

BLAST of MS003445 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of MS003445 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
W9SNS4	2.2e-21	70.59	Uncharacterized protein OS=Morus notabilis OX=981085 GN=L484_010408 PE=4 SV=1	[more]
A0A061FTQ6	2.9e-21	73.13	Cytoplasmic tRNA 2-thiolation protein 1 OS=Theobroma cacao OX=3641 GN=TCM_042740...	[more]
A0A200R4Z5	2.9e-21	68.57	Uncharacterized protein OS=Macleaya cordata OX=56857 GN=BVC80_1835g174 PE=4 SV=1	[more]
A0A2P5ALC9	4.9e-21	72.46	Uncharacterized protein OS=Parasponia andersonii OX=3476 GN=PanWU01x14_320780 PE...	[more]
A0A1R3I234	4.9e-21	80.70	Uncharacterized protein OS=Corchorus olitorius OX=93759 GN=COLO4_25500 PE=4 SV=1	[more]

BLAST of MS003445 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G55570.1	2.0e-22	68.66	unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA...	[more]
AT5G41761.1	5.0e-18	63.33	unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic...	[more]
AT3G09950.1	1.0e-15	45.98	unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA...	[more]
AT5G55620.1	1.7e-13	52.17	unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA...	[more]
AT3G11405.1	1.1e-12	61.82	unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA...	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PANTHER	PTHR33513:SF24	CYTOPLASMIC TRNA 2-THIOLATION PROTEIN-RELATED	coord: 8..89
None	No IPR available	PANTHER	PTHR33513	OS06G0523300 PROTEIN	coord: 8..89

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS003445.1	MS003445.1	mRNA