MS003354 (gene) Bitter gourd (TR) v1

Overview


Name	MS003354
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	RING-H2 finger protein ATL74
Location	scaffold234: 2312260 .. 2312859 (-)
RNA-Seq Expression	MS003354
Synteny	MS003354

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTGTTCGATCACCACCGTCACCGTCCCCACCGCCTCCTCCTCGACGGCGACGCGACGGCGCCGCCGTCGAATGGAAACAGAACGCGAAGTTCGTACAGCAACGAAGCGAATTTCGACACGAATATGGTGATAATCCTCGCGGCGTTACTCTGCGCGCTGATTTGCGCTCTCGGACTGAATTCGATCGTCCGCTGCGCCCTCCGCTGCAGCCGGCGGTTCGCCTTCGAGACGGCCGGCGGCACATCCACGCGCCTCGCCGCCACGGGGCTGAAGAAAAGCGCATTACGCCAAATTCCGGTGGCCGTGTACGGGTCGGAGTCTGGTCTAGAGATCCGAGAAACGGACTGCCCTATCTGCTTGGGAGATTTCATCGCCGGCGAGAAAATCAAAGTTCTGCCCAAATGTAACCACGAGTTTCACGTACGATGTATCGACACGTGGTTGGCTTCCCACTCGTCTTGTCCGACATGTCGTCAGTCGCTGCTCGAACAACAGTCGTGTTCTGAATCGGCGGTAGCTGAAGCCGGAAACCGACCCGCCGGAAATTCGTCGGCCGGCGGCCAAGGTGAGATCTCGATTACTGTAGATGAAATTAGC

mRNA sequence

ATGGTGTTCGATCACCACCGTCACCGTCCCCACCGCCTCCTCCTCGACGGCGACGCGACGGCGCCGCCGTCGAATGGAAACAGAACGCGAAGTTCGTACAGCAACGAAGCGAATTTCGACACGAATATGGTGATAATCCTCGCGGCGTTACTCTGCGCGCTGATTTGCGCTCTCGGACTGAATTCGATCGTCCGCTGCGCCCTCCGCTGCAGCCGGCGGTTCGCCTTCGAGACGGCCGGCGGCACATCCACGCGCCTCGCCGCCACGGGGCTGAAGAAAAGCGCATTACGCCAAATTCCGGTGGCCGTGTACGGGTCGGAGTCTGGTCTAGAGATCCGAGAAACGGACTGCCCTATCTGCTTGGGAGATTTCATCGCCGGCGAGAAAATCAAAGTTCTGCCCAAATGTAACCACGAGTTTCACGTACGATGTATCGACACGTGGTTGGCTTCCCACTCGTCTTGTCCGACATGTCGTCAGTCGCTGCTCGAACAACAGTCGTGTTCTGAATCGGCGGTAGCTGAAGCCGGAAACCGACCCGCCGGAAATTCGTCGGCCGGCGGCCAAGGTGAGATCTCGATTACTGTAGATGAAATTAGC

Coding sequence (CDS)

ATGGTGTTCGATCACCACCGTCACCGTCCCCACCGCCTCCTCCTCGACGGCGACGCGACGGCGCCGCCGTCGAATGGAAACAGAACGCGAAGTTCGTACAGCAACGAAGCGAATTTCGACACGAATATGGTGATAATCCTCGCGGCGTTACTCTGCGCGCTGATTTGCGCTCTCGGACTGAATTCGATCGTCCGCTGCGCCCTCCGCTGCAGCCGGCGGTTCGCCTTCGAGACGGCCGGCGGCACATCCACGCGCCTCGCCGCCACGGGGCTGAAGAAAAGCGCATTACGCCAAATTCCGGTGGCCGTGTACGGGTCGGAGTCTGGTCTAGAGATCCGAGAAACGGACTGCCCTATCTGCTTGGGAGATTTCATCGCCGGCGAGAAAATCAAAGTTCTGCCCAAATGTAACCACGAGTTTCACGTACGATGTATCGACACGTGGTTGGCTTCCCACTCGTCTTGTCCGACATGTCGTCAGTCGCTGCTCGAACAACAGTCGTGTTCTGAATCGGCGGTAGCTGAAGCCGGAAACCGACCCGCCGGAAATTCGTCGGCCGGCGGCCAAGGTGAGATCTCGATTACTGTAGATGAAATTAGC

Protein sequence

MVFDHHRHRPHRLLLDGDATAPPSNGNRTRSSYSNEANFDTNMVIILAALLCALICALGLNSIVRCALRCSRRFAFETAGGTSTRLAATGLKKSALRQIPVAVYGSESGLEIRETDCPICLGDFIAGEKIKVLPKCNHEFHVRCIDTWLASHSSCPTCRQSLLEQQSCSESAVAEAGNRPAGNSSAGGQGEISITVDEIS

Homology

[Close]

BLAST of MS003354 vs. NCBI nr
Match: XP_022134542.1 (RING-H2 finger protein ATL74 [Momordica charantia])

HSP 1 Score: 375.2 bits (962), Expect = 3.6e-100
Identity = 187/190 (98.42%), Postives = 187/190 (98.42%), Query Frame = 0


Query: 1   MVFDHHRHRPHRLLLDGDATAPPSNGNRTRSSYSNEANFDTNMVIILAALLCALICALGL 60
           M  DHHRHRPHR LLDGDATAPPSNGNRTRSSYSNEANFDTNMVIILAALLCALICALGL
Sbjct: 3   MASDHHRHRPHRFLLDGDATAPPSNGNRTRSSYSNEANFDTNMVIILAALLCALICALGL 62

Query: 61  NSIVRCALRCSRRFAFETAGGTSTRLAATGLKKSALRQIPVAVYGSESGLEIRETDCPIC 120
           NSIVRCALRCSRRFAFETAGGTSTRLAATGLKKSALRQIPVAVYGSESGLEIRETDCPIC
Sbjct: 63  NSIVRCALRCSRRFAFETAGGTSTRLAATGLKKSALRQIPVAVYGSESGLEIRETDCPIC 122

Query: 121 LGDFIAGEKIKVLPKCNHEFHVRCIDTWLASHSSCPTCRQSLLEQQSCSESAVAEAGNRP 180
           LGDFIAGEKIKVLPKCNHEFHVRCIDTWLASHSSCPTCRQSLLEQQSCSESAVAEAGNRP
Sbjct: 123 LGDFIAGEKIKVLPKCNHEFHVRCIDTWLASHSSCPTCRQSLLEQQSCSESAVAEAGNRP 182

Query: 181 AGNSSAGGQG 191
           AGNSSAGGQG
Sbjct: 183 AGNSSAGGQG 192

[Close]

BLAST of MS003354 vs. NCBI nr
Match: KAA0052006.1 (RING-H2 finger protein ATL72 [Cucumis melo var. makuwa] >TYK04553.1 RING-H2 finger protein ATL72 [Cucumis melo var. makuwa])

HSP 1 Score: 351.3 bits (900), Expect = 5.6e-93
Identity = 177/200 (88.50%), Postives = 187/200 (93.50%), Query Frame = 0


Query: 1   MVFDHHRHRPHRLLLDGDATAPPSNGNRTRSSYSNEANFDTNMVIILAALLCALICALGL 60
           M  +HHR R HRLLLD D TA PSNG+RTR+SY+NEANFDTNMVIILAALLCALICALGL
Sbjct: 1   MESNHHRPRSHRLLLDADTTAAPSNGSRTRNSYNNEANFDTNMVIILAALLCALICALGL 60

Query: 61  NSIVRCALRCSRRFAFETAGGTSTRLAATGLKKSALRQIPVAVYGSESGLEIRETDCPIC 120
           NSIVRCALRCSRRFAFET GGT++RL A+GLKKSALRQIPVAVYGSE+GLEIRETDCPIC
Sbjct: 61  NSIVRCALRCSRRFAFETGGGTASRLTASGLKKSALRQIPVAVYGSETGLEIRETDCPIC 120

Query: 121 LGDFIAGEKIKVLPKCNHEFHVRCIDTWLASHSSCPTCRQSLLEQQSCSESAVAEAGNRP 180
           LGDFIAGEKIKVLPKCNH FHVRCIDTWLASHSSCPTCRQSL+EQQS SESA AEAGNRP
Sbjct: 121 LGDFIAGEKIKVLPKCNHGFHVRCIDTWLASHSSCPTCRQSLVEQQSVSESAGAEAGNRP 180

Query: 181 AGNSSAGGQGEISITVDEIS 201
           AGNSSA GQGEISITVDEI+
Sbjct: 181 AGNSSASGQGEISITVDEIT 200

[Close]

BLAST of MS003354 vs. NCBI nr
Match: KAG7016045.1 (RING-H2 finger protein ATL72, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 346.3 bits (887), Expect = 1.8e-91
Identity = 172/200 (86.00%), Postives = 184/200 (92.00%), Query Frame = 0


Query: 1   MVFDHHRHRPHRLLLDGDATAPPSNGNRTRSSYSNEANFDTNMVIILAALLCALICALGL 60
           MV DHHR RPHRLLLD + T  PSNG+RTR+SY+NEANFDTNMVIILAALLCALICALGL
Sbjct: 45  MVSDHHRPRPHRLLLDANTTEAPSNGSRTRNSYTNEANFDTNMVIILAALLCALICALGL 104

Query: 61  NSIVRCALRCSRRFAFETAGGTSTRLAATGLKKSALRQIPVAVYGSESGLEIRETDCPIC 120
           NSIVRCALRCSRRF+FET GGT++RLAATGLKKSALR++PV VYGSESGL IRETDCPIC
Sbjct: 105 NSIVRCALRCSRRFSFETGGGTASRLAATGLKKSALRKLPVTVYGSESGLGIRETDCPIC 164

Query: 121 LGDFIAGEKIKVLPKCNHEFHVRCIDTWLASHSSCPTCRQSLLEQQSCSESAVAEAGNRP 180
           LGDFIAGEKIKVLPKCNH FHVRCIDTWLASHSSCPTCRQSL EQQS SES  A+AGNRP
Sbjct: 165 LGDFIAGEKIKVLPKCNHGFHVRCIDTWLASHSSCPTCRQSLFEQQSASESTEADAGNRP 224

Query: 181 AGNSSAGGQGEISITVDEIS 201
           AGNSSA GQGEISI +D+IS
Sbjct: 225 AGNSSASGQGEISIPIDQIS 244

[Close]

BLAST of MS003354 vs. NCBI nr
Match: XP_023550807.1 (RING-H2 finger protein ATL74-like [Cucurbita pepo subsp. pepo] >XP_023550808.1 RING-H2 finger protein ATL74-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 346.3 bits (887), Expect = 1.8e-91
Identity = 172/200 (86.00%), Postives = 184/200 (92.00%), Query Frame = 0


Query: 1   MVFDHHRHRPHRLLLDGDATAPPSNGNRTRSSYSNEANFDTNMVIILAALLCALICALGL 60
           MV DHHR RPHRLLLD + T  PSNG+RTR+SY+NEANFDTNMVIILAALLCALICALGL
Sbjct: 3   MVSDHHRPRPHRLLLDANTTEAPSNGSRTRNSYTNEANFDTNMVIILAALLCALICALGL 62

Query: 61  NSIVRCALRCSRRFAFETAGGTSTRLAATGLKKSALRQIPVAVYGSESGLEIRETDCPIC 120
           NSIVRCALRCSRRF+FET GGT++RLAATGLKKSALR++PV VYGSESGL IRETDCPIC
Sbjct: 63  NSIVRCALRCSRRFSFETGGGTASRLAATGLKKSALRKLPVTVYGSESGLGIRETDCPIC 122

Query: 121 LGDFIAGEKIKVLPKCNHEFHVRCIDTWLASHSSCPTCRQSLLEQQSCSESAVAEAGNRP 180
           LGDFIAGEKIKVLPKCNH FHVRCIDTWLASHSSCPTCRQSL EQQS SES  A+AGNRP
Sbjct: 123 LGDFIAGEKIKVLPKCNHGFHVRCIDTWLASHSSCPTCRQSLFEQQSASESTEADAGNRP 182

Query: 181 AGNSSAGGQGEISITVDEIS 201
           AGNSSA GQGEISI +D+IS
Sbjct: 183 AGNSSASGQGEISIPIDQIS 202

[Close]

BLAST of MS003354 vs. NCBI nr
Match: XP_022938777.1 (RING-H2 finger protein ATL74-like [Cucurbita moschata])

HSP 1 Score: 344.7 bits (883), Expect = 5.2e-91
Identity = 171/200 (85.50%), Postives = 183/200 (91.50%), Query Frame = 0


Query: 1   MVFDHHRHRPHRLLLDGDATAPPSNGNRTRSSYSNEANFDTNMVIILAALLCALICALGL 60
           MV DHHR RPHRLLLD + T  PSNG+RTR+SY+NEANFDTNMVIILAALLCALICALGL
Sbjct: 3   MVSDHHRPRPHRLLLDANTTEAPSNGSRTRNSYTNEANFDTNMVIILAALLCALICALGL 62

Query: 61  NSIVRCALRCSRRFAFETAGGTSTRLAATGLKKSALRQIPVAVYGSESGLEIRETDCPIC 120
           NSIVRCA RCSRRF+FET GGT++RLAATGLKKSALR++PV VYGSESGL IRETDCPIC
Sbjct: 63  NSIVRCAFRCSRRFSFETGGGTASRLAATGLKKSALRKLPVTVYGSESGLGIRETDCPIC 122

Query: 121 LGDFIAGEKIKVLPKCNHEFHVRCIDTWLASHSSCPTCRQSLLEQQSCSESAVAEAGNRP 180
           LGDFIAGEKIKVLPKCNH FHVRCIDTWLASHSSCPTCRQSL EQQS SES  A+AGNRP
Sbjct: 123 LGDFIAGEKIKVLPKCNHGFHVRCIDTWLASHSSCPTCRQSLFEQQSASESTEADAGNRP 182

Query: 181 AGNSSAGGQGEISITVDEIS 201
           AGNSSA GQGEISI +D+IS
Sbjct: 183 AGNSSASGQGEISIPIDQIS 202

[Close]

BLAST of MS003354 vs. ExPASy Swiss-Prot
Match: Q9SG96 (RING-H2 finger protein ATL72 OS=Arabidopsis thaliana OX=3702 GN=ATL72 PE=2 SV=1)

HSP 1 Score: 179.9 bits (455), Expect = 2.9e-44
Identity = 100/163 (61.35%), Postives = 116/163 (71.17%), Query Frame = 0


Query: 12  RLLLDGDATAPPSNGNRTRSSYSNEANFDTNMVIILAALLCALICALGLNSIVRCALRCS 71
           RLLL+  A A P+N N       N+  FDTNMVIILAALLCALICAL LNS +RC LR +
Sbjct: 3   RLLLEPQANA-PANANPKPKGGINDTYFDTNMVIILAALLCALICALSLNSALRCVLRIT 62

Query: 72  RRFAFE-------TAGGTSTRL-AATGLKKSALRQIPVAVYGSESGLEIRETDCPICLGD 131
           RRF  +        A     RL AATGLKK AL+QIPV +YGS   ++++ T+C ICLGD
Sbjct: 63  RRFTSDDQVSNASNANANLGRLAAATGLKKQALKQIPVGLYGS-GIIDMKATECLICLGD 122

Query: 132 FIAGEKIKVLPKCNHEFHVRCIDTWLASHSSCPTCRQSLLEQQ 167
           F  GEK++VLPKCNH FHVRCIDTWL S SSCPTCRQSLL +Q
Sbjct: 123 FEDGEKVRVLPKCNHGFHVRCIDTWLLSRSSCPTCRQSLLLEQ 163

[Close]

BLAST of MS003354 vs. ExPASy Swiss-Prot
Match: Q9FLC6 (RING-H2 finger protein ATL73 OS=Arabidopsis thaliana OX=3702 GN=ATL73 PE=2 SV=1)

HSP 1 Score: 172.2 bits (435), Expect = 6.1e-42
Identity = 85/137 (62.04%), Postives = 104/137 (75.91%), Query Frame = 0


Query: 31  SSYSNEANFDTNMVIILAALLCALICALGLNSIVRCALRCSRRFAFETAGGTSTRLAATG 90
           S+  N A+ DT+MVIILAALLCALICALG+NS++RC LRC+RRF        +    A G
Sbjct: 29  SNNKNIASMDTHMVIILAALLCALICALGINSVLRCVLRCTRRFTPNEDPVDTNANVAKG 88

Query: 91  LKKSALRQIPVAVYGSESGLEIRETDCPICLGDFIAGEKIKVLPKCNHEFHVRCIDTWLA 150
           +KK AL+ IPV  Y  E  L+++ T+C ICLGDF+ GE ++VLPKCNH FHV+CIDTWL 
Sbjct: 89  IKKRALKVIPVDSYSPE--LKMKATECLICLGDFVEGETVRVLPKCNHGFHVKCIDTWLL 148

Query: 151 SHSSCPTCRQSLLEQQS 168
           SHSSCPTCRQSLLE Q+
Sbjct: 149 SHSSCPTCRQSLLEHQT 163

[Close]

BLAST of MS003354 vs. ExPASy Swiss-Prot
Match: Q9LZV8 (RING-H2 finger protein ATL74 OS=Arabidopsis thaliana OX=3702 GN=ATL74 PE=2 SV=1)

HSP 1 Score: 169.5 bits (428), Expect = 3.9e-41
Identity = 86/154 (55.84%), Postives = 106/154 (68.83%), Query Frame = 0


Query: 11  HRLLLDGDATAPPSNGNRTRSSYSNEANFDTNMVIILAALLCALICALGLNSIVRCALRC 70
           HRLLL+       ++G+     Y+ + NFD NMVIILAALLCALI ALGLNSI+RCA+RC
Sbjct: 2   HRLLLESHGGGNETSGSGGGDGYTRDMNFDANMVIILAALLCALILALGLNSILRCAMRC 61

Query: 71  SRRFAFETAGGTSTRLAATGLKKSALRQIPVAVYGSESGLEIRETDCPICLGDFIAGEKI 130
               +   A GT    A  GLKK  L++ PVA YGS   ++I  T+C ICLG+F  GE++
Sbjct: 62  GFGLSSSAAAGTVADRA--GLKKRELKKFPVAEYGS-GEVKIAATECAICLGEFADGERV 121

Query: 131 KVLPKCNHEFHVRCIDTWLASHSSCPTCRQSLLE 165
           +VLP CNH FH+ CIDTWL SHSSCP CR SL+E
Sbjct: 122 RVLPPCNHSFHMSCIDTWLVSHSSCPNCRHSLIE 152

[Close]

BLAST of MS003354 vs. ExPASy Swiss-Prot
Match: Q6NQG7 (RING-H2 finger protein ATL78 OS=Arabidopsis thaliana OX=3702 GN=ATL78 PE=2 SV=1)

HSP 1 Score: 152.9 bits (385), Expect = 3.8e-36
Identity = 78/167 (46.71%), Postives = 108/167 (64.67%), Query Frame = 0


Query: 31  SSYSNEANFDTNMVIILAALLCALICALGLNSIVRCALRCSRRFAFETAGGT-STRLAAT 90
           S Y  + NFD N+V++L+ LLCAL+C+LGLNSI+RCALRCS     E  G     RL  T
Sbjct: 44  SPYVGDNNFDANVVMVLSVLLCALVCSLGLNSIIRCALRCSNLVPSEAGGDNYPVRLTNT 103

Query: 91  GLKKSALRQIPVAVYGSESGLEIRETDCPICLGDFIAGEKIKVLPKCNHEFHVRCIDTWL 150
           G+K+ AL+      Y +E  L   +T+C ICL +F+A E++K+LP C+H FHVRCID WL
Sbjct: 104 GVKRKALKSFQTVSYSTELNLPGLDTECAICLSEFVAEERVKLLPTCHHGFHVRCIDKWL 163

Query: 151 ASHSSCPTCRQSLLEQQSCSESAVAEAGNRPAGNSSAGGQGEISITV 197
           +SHSSCPTCR  L+  Q+C +  +A+     + NS+   Q  I + +
Sbjct: 164 SSHSSCPTCRHCLI--QTCEK--IADCSQTSSLNSTQPPQDSIILQI 206

[Close]

BLAST of MS003354 vs. ExPASy Swiss-Prot
Match: P0C034 (RING-H2 finger protein ATL10 OS=Arabidopsis thaliana OX=3702 GN=ATL10 PE=2 SV=1)

HSP 1 Score: 131.7 bits (330), Expect = 9.1e-30
Identity = 64/134 (47.76%), Postives = 89/134 (66.42%), Query Frame = 0


Query: 34  SNEANFDTNMVIILAALLCALICALGLNSIVRCALRCSRRFAFE--TAGGTSTRLAAT-G 93
           ++E N   N++++L+ L+C +IC LGL+ I+RCALR S RF         +STR ++  G
Sbjct: 49  THENNLSGNVMMLLSILICGIICCLGLHYIIRCALRRSTRFMISEPVPSLSSTRGSSNKG 108

Query: 94  LKKSALRQIPVAVYGSESGLEIRETDCPICLGDFIAGEKIKVLPKCNHEFHVRCIDTWLA 153
           +KK ALR  PV  Y  E  L   + +C ICL DF++GE++++LPKCNH FHVRCID WL 
Sbjct: 109 IKKKALRMFPVVSYSPEMNLPGLDEECVICLSDFVSGEQLRLLPKCNHGFHVRCIDKWLQ 168

Query: 154 SHSSCPTCRQSLLE 165
            H +CP CR  L+E
Sbjct: 169 QHLTCPKCRNCLVE 182

[Close]

BLAST of MS003354 vs. ExPASy TrEMBL
Match: A0A6J1BZW4 (RING-H2 finger protein ATL74 OS=Momordica charantia OX=3673 GN=LOC111006761 PE=4 SV=1)

HSP 1 Score: 375.2 bits (962), Expect = 1.7e-100
Identity = 187/190 (98.42%), Postives = 187/190 (98.42%), Query Frame = 0


Query: 1   MVFDHHRHRPHRLLLDGDATAPPSNGNRTRSSYSNEANFDTNMVIILAALLCALICALGL 60
           M  DHHRHRPHR LLDGDATAPPSNGNRTRSSYSNEANFDTNMVIILAALLCALICALGL
Sbjct: 3   MASDHHRHRPHRFLLDGDATAPPSNGNRTRSSYSNEANFDTNMVIILAALLCALICALGL 62

Query: 61  NSIVRCALRCSRRFAFETAGGTSTRLAATGLKKSALRQIPVAVYGSESGLEIRETDCPIC 120
           NSIVRCALRCSRRFAFETAGGTSTRLAATGLKKSALRQIPVAVYGSESGLEIRETDCPIC
Sbjct: 63  NSIVRCALRCSRRFAFETAGGTSTRLAATGLKKSALRQIPVAVYGSESGLEIRETDCPIC 122

Query: 121 LGDFIAGEKIKVLPKCNHEFHVRCIDTWLASHSSCPTCRQSLLEQQSCSESAVAEAGNRP 180
           LGDFIAGEKIKVLPKCNHEFHVRCIDTWLASHSSCPTCRQSLLEQQSCSESAVAEAGNRP
Sbjct: 123 LGDFIAGEKIKVLPKCNHEFHVRCIDTWLASHSSCPTCRQSLLEQQSCSESAVAEAGNRP 182

Query: 181 AGNSSAGGQG 191
           AGNSSAGGQG
Sbjct: 183 AGNSSAGGQG 192

[Close]

BLAST of MS003354 vs. ExPASy TrEMBL
Match: A0A5D3BXT6 (RING-H2 finger protein ATL72 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold409G001810 PE=4 SV=1)

HSP 1 Score: 351.3 bits (900), Expect = 2.7e-93
Identity = 177/200 (88.50%), Postives = 187/200 (93.50%), Query Frame = 0


Query: 1   MVFDHHRHRPHRLLLDGDATAPPSNGNRTRSSYSNEANFDTNMVIILAALLCALICALGL 60
           M  +HHR R HRLLLD D TA PSNG+RTR+SY+NEANFDTNMVIILAALLCALICALGL
Sbjct: 1   MESNHHRPRSHRLLLDADTTAAPSNGSRTRNSYNNEANFDTNMVIILAALLCALICALGL 60

Query: 61  NSIVRCALRCSRRFAFETAGGTSTRLAATGLKKSALRQIPVAVYGSESGLEIRETDCPIC 120
           NSIVRCALRCSRRFAFET GGT++RL A+GLKKSALRQIPVAVYGSE+GLEIRETDCPIC
Sbjct: 61  NSIVRCALRCSRRFAFETGGGTASRLTASGLKKSALRQIPVAVYGSETGLEIRETDCPIC 120

Query: 121 LGDFIAGEKIKVLPKCNHEFHVRCIDTWLASHSSCPTCRQSLLEQQSCSESAVAEAGNRP 180
           LGDFIAGEKIKVLPKCNH FHVRCIDTWLASHSSCPTCRQSL+EQQS SESA AEAGNRP
Sbjct: 121 LGDFIAGEKIKVLPKCNHGFHVRCIDTWLASHSSCPTCRQSLVEQQSVSESAGAEAGNRP 180

Query: 181 AGNSSAGGQGEISITVDEIS 201
           AGNSSA GQGEISITVDEI+
Sbjct: 181 AGNSSASGQGEISITVDEIT 200

[Close]

BLAST of MS003354 vs. ExPASy TrEMBL
Match: A0A0A0KAH2 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G014550 PE=4 SV=1)

HSP 1 Score: 349.0 bits (894), Expect = 1.3e-92
Identity = 176/200 (88.00%), Postives = 186/200 (93.00%), Query Frame = 0


Query: 1   MVFDHHRHRPHRLLLDGDATAPPSNGNRTRSSYSNEANFDTNMVIILAALLCALICALGL 60
           M  +HHR RP RLLLD D TAPPSNG+RTR+SY+NEANFDTNMVIILAALLCALICALGL
Sbjct: 3   MESNHHRPRPPRLLLDPDTTAPPSNGSRTRNSYNNEANFDTNMVIILAALLCALICALGL 62

Query: 61  NSIVRCALRCSRRFAFETAGGTSTRLAATGLKKSALRQIPVAVYGSESGLEIRETDCPIC 120
           NSIVRCALRCSRRFAFET GGT++RL ATGLKKSALRQIPVAVYGSE+GLEIRETDCPIC
Sbjct: 63  NSIVRCALRCSRRFAFETGGGTASRLTATGLKKSALRQIPVAVYGSETGLEIRETDCPIC 122

Query: 121 LGDFIAGEKIKVLPKCNHEFHVRCIDTWLASHSSCPTCRQSLLEQQSCSESAVAEAGNRP 180
           LGDF+AGEKIK+LPKCNH FHVRCIDTWLASHSSCPTCRQSLLEQQS SESAV EAGNR 
Sbjct: 123 LGDFMAGEKIKILPKCNHGFHVRCIDTWLASHSSCPTCRQSLLEQQSVSESAVTEAGNRG 182

Query: 181 AGNSSAGGQGEISITVDEIS 201
           AGNSSA GQGEISITVD I+
Sbjct: 183 AGNSSASGQGEISITVDGIT 202

[Close]

BLAST of MS003354 vs. ExPASy TrEMBL
Match: A0A6J1FF16 (RING-H2 finger protein ATL74-like OS=Cucurbita moschata OX=3662 GN=LOC111444892 PE=4 SV=1)

HSP 1 Score: 344.7 bits (883), Expect = 2.5e-91
Identity = 171/200 (85.50%), Postives = 183/200 (91.50%), Query Frame = 0


Query: 1   MVFDHHRHRPHRLLLDGDATAPPSNGNRTRSSYSNEANFDTNMVIILAALLCALICALGL 60
           MV DHHR RPHRLLLD + T  PSNG+RTR+SY+NEANFDTNMVIILAALLCALICALGL
Sbjct: 3   MVSDHHRPRPHRLLLDANTTEAPSNGSRTRNSYTNEANFDTNMVIILAALLCALICALGL 62

Query: 61  NSIVRCALRCSRRFAFETAGGTSTRLAATGLKKSALRQIPVAVYGSESGLEIRETDCPIC 120
           NSIVRCA RCSRRF+FET GGT++RLAATGLKKSALR++PV VYGSESGL IRETDCPIC
Sbjct: 63  NSIVRCAFRCSRRFSFETGGGTASRLAATGLKKSALRKLPVTVYGSESGLGIRETDCPIC 122

Query: 121 LGDFIAGEKIKVLPKCNHEFHVRCIDTWLASHSSCPTCRQSLLEQQSCSESAVAEAGNRP 180
           LGDFIAGEKIKVLPKCNH FHVRCIDTWLASHSSCPTCRQSL EQQS SES  A+AGNRP
Sbjct: 123 LGDFIAGEKIKVLPKCNHGFHVRCIDTWLASHSSCPTCRQSLFEQQSASESTEADAGNRP 182

Query: 181 AGNSSAGGQGEISITVDEIS 201
           AGNSSA GQGEISI +D+IS
Sbjct: 183 AGNSSASGQGEISIPIDQIS 202

[Close]

BLAST of MS003354 vs. ExPASy TrEMBL
Match: A0A6J1JVF4 (RING-H2 finger protein ATL74-like OS=Cucurbita maxima OX=3661 GN=LOC111489233 PE=4 SV=1)

HSP 1 Score: 344.4 bits (882), Expect = 3.3e-91
Identity = 171/200 (85.50%), Postives = 183/200 (91.50%), Query Frame = 0


Query: 1   MVFDHHRHRPHRLLLDGDATAPPSNGNRTRSSYSNEANFDTNMVIILAALLCALICALGL 60
           MV DHHR RPHRLLLD + T  PSNG+RTR+SY+NEANFDTNMVIILAALLCALICALGL
Sbjct: 3   MVSDHHRPRPHRLLLDANTTEAPSNGSRTRNSYTNEANFDTNMVIILAALLCALICALGL 62

Query: 61  NSIVRCALRCSRRFAFETAGGTSTRLAATGLKKSALRQIPVAVYGSESGLEIRETDCPIC 120
           NSIVRCALRCSRRF+FET GGT++RLAATGLKKSALR++PV VYGSESGL IRETDCPIC
Sbjct: 63  NSIVRCALRCSRRFSFETGGGTASRLAATGLKKSALRKLPVTVYGSESGLGIRETDCPIC 122

Query: 121 LGDFIAGEKIKVLPKCNHEFHVRCIDTWLASHSSCPTCRQSLLEQQSCSESAVAEAGNRP 180
           LGDFIAGEKIKVLPKCNH FHVRCIDTWLASHSSCPTCRQSL EQQS SES  A+AGNRP
Sbjct: 123 LGDFIAGEKIKVLPKCNHGFHVRCIDTWLASHSSCPTCRQSLFEQQSASESTEADAGNRP 182

Query: 181 AGNSSAGGQGEISITVDEIS 201
           AGN SA GQGEISI +D+IS
Sbjct: 183 AGNPSASGQGEISIPIDQIS 202

[Close]

BLAST of MS003354 vs. TAIR 10
Match: AT3G10910.1 (RING/U-box superfamily protein )

HSP 1 Score: 179.9 bits (455), Expect = 2.1e-45
Identity = 100/163 (61.35%), Postives = 116/163 (71.17%), Query Frame = 0


Query: 12  RLLLDGDATAPPSNGNRTRSSYSNEANFDTNMVIILAALLCALICALGLNSIVRCALRCS 71
           RLLL+  A A P+N N       N+  FDTNMVIILAALLCALICAL LNS +RC LR +
Sbjct: 3   RLLLEPQANA-PANANPKPKGGINDTYFDTNMVIILAALLCALICALSLNSALRCVLRIT 62

Query: 72  RRFAFE-------TAGGTSTRL-AATGLKKSALRQIPVAVYGSESGLEIRETDCPICLGD 131
           RRF  +        A     RL AATGLKK AL+QIPV +YGS   ++++ T+C ICLGD
Sbjct: 63  RRFTSDDQVSNASNANANLGRLAAATGLKKQALKQIPVGLYGS-GIIDMKATECLICLGD 122

Query: 132 FIAGEKIKVLPKCNHEFHVRCIDTWLASHSSCPTCRQSLLEQQ 167
           F  GEK++VLPKCNH FHVRCIDTWL S SSCPTCRQSLL +Q
Sbjct: 123 FEDGEKVRVLPKCNHGFHVRCIDTWLLSRSSCPTCRQSLLLEQ 163

[Close]

BLAST of MS003354 vs. TAIR 10
Match: AT5G05280.1 (RING/U-box superfamily protein )

HSP 1 Score: 172.2 bits (435), Expect = 4.3e-43
Identity = 85/137 (62.04%), Postives = 104/137 (75.91%), Query Frame = 0


Query: 31  SSYSNEANFDTNMVIILAALLCALICALGLNSIVRCALRCSRRFAFETAGGTSTRLAATG 90
           S+  N A+ DT+MVIILAALLCALICALG+NS++RC LRC+RRF        +    A G
Sbjct: 29  SNNKNIASMDTHMVIILAALLCALICALGINSVLRCVLRCTRRFTPNEDPVDTNANVAKG 88

Query: 91  LKKSALRQIPVAVYGSESGLEIRETDCPICLGDFIAGEKIKVLPKCNHEFHVRCIDTWLA 150
           +KK AL+ IPV  Y  E  L+++ T+C ICLGDF+ GE ++VLPKCNH FHV+CIDTWL 
Sbjct: 89  IKKRALKVIPVDSYSPE--LKMKATECLICLGDFVEGETVRVLPKCNHGFHVKCIDTWLL 148

Query: 151 SHSSCPTCRQSLLEQQS 168
           SHSSCPTCRQSLLE Q+
Sbjct: 149 SHSSCPTCRQSLLEHQT 163

[Close]

BLAST of MS003354 vs. TAIR 10
Match: AT5G01880.1 (RING/U-box superfamily protein )

HSP 1 Score: 169.5 bits (428), Expect = 2.8e-42
Identity = 86/154 (55.84%), Postives = 106/154 (68.83%), Query Frame = 0


Query: 11  HRLLLDGDATAPPSNGNRTRSSYSNEANFDTNMVIILAALLCALICALGLNSIVRCALRC 70
           HRLLL+       ++G+     Y+ + NFD NMVIILAALLCALI ALGLNSI+RCA+RC
Sbjct: 2   HRLLLESHGGGNETSGSGGGDGYTRDMNFDANMVIILAALLCALILALGLNSILRCAMRC 61

Query: 71  SRRFAFETAGGTSTRLAATGLKKSALRQIPVAVYGSESGLEIRETDCPICLGDFIAGEKI 130
               +   A GT    A  GLKK  L++ PVA YGS   ++I  T+C ICLG+F  GE++
Sbjct: 62  GFGLSSSAAAGTVADRA--GLKKRELKKFPVAEYGS-GEVKIAATECAICLGEFADGERV 121

Query: 131 KVLPKCNHEFHVRCIDTWLASHSSCPTCRQSLLE 165
           +VLP CNH FH+ CIDTWL SHSSCP CR SL+E
Sbjct: 122 RVLPPCNHSFHMSCIDTWLVSHSSCPNCRHSLIE 152

[Close]

BLAST of MS003354 vs. TAIR 10
Match: AT1G49230.1 (RING/U-box superfamily protein )

HSP 1 Score: 152.9 bits (385), Expect = 2.7e-37
Identity = 78/167 (46.71%), Postives = 108/167 (64.67%), Query Frame = 0


Query: 31  SSYSNEANFDTNMVIILAALLCALICALGLNSIVRCALRCSRRFAFETAGGT-STRLAAT 90
           S Y  + NFD N+V++L+ LLCAL+C+LGLNSI+RCALRCS     E  G     RL  T
Sbjct: 44  SPYVGDNNFDANVVMVLSVLLCALVCSLGLNSIIRCALRCSNLVPSEAGGDNYPVRLTNT 103

Query: 91  GLKKSALRQIPVAVYGSESGLEIRETDCPICLGDFIAGEKIKVLPKCNHEFHVRCIDTWL 150
           G+K+ AL+      Y +E  L   +T+C ICL +F+A E++K+LP C+H FHVRCID WL
Sbjct: 104 GVKRKALKSFQTVSYSTELNLPGLDTECAICLSEFVAEERVKLLPTCHHGFHVRCIDKWL 163

Query: 151 ASHSSCPTCRQSLLEQQSCSESAVAEAGNRPAGNSSAGGQGEISITV 197
           +SHSSCPTCR  L+  Q+C +  +A+     + NS+   Q  I + +
Sbjct: 164 SSHSSCPTCRHCLI--QTCEK--IADCSQTSSLNSTQPPQDSIILQI 206

[Close]

BLAST of MS003354 vs. TAIR 10
Match: AT1G49220.1 (RING/U-box superfamily protein )

HSP 1 Score: 131.7 bits (330), Expect = 6.4e-31
Identity = 64/134 (47.76%), Postives = 89/134 (66.42%), Query Frame = 0


Query: 34  SNEANFDTNMVIILAALLCALICALGLNSIVRCALRCSRRFAFE--TAGGTSTRLAAT-G 93
           ++E N   N++++L+ L+C +IC LGL+ I+RCALR S RF         +STR ++  G
Sbjct: 49  THENNLSGNVMMLLSILICGIICCLGLHYIIRCALRRSTRFMISEPVPSLSSTRGSSNKG 108

Query: 94  LKKSALRQIPVAVYGSESGLEIRETDCPICLGDFIAGEKIKVLPKCNHEFHVRCIDTWLA 153
           +KK ALR  PV  Y  E  L   + +C ICL DF++GE++++LPKCNH FHVRCID WL 
Sbjct: 109 IKKKALRMFPVVSYSPEMNLPGLDEECVICLSDFVSGEQLRLLPKCNHGFHVRCIDKWLQ 168

Query: 154 SHSSCPTCRQSLLE 165
            H +CP CR  L+E
Sbjct: 169 QHLTCPKCRNCLVE 182

The following BLAST results are available for this feature:

BLAST of MS003354 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022134542.1	3.6e-100	98.42	RING-H2 finger protein ATL74 [Momordica charantia]	[more]
KAA0052006.1	5.6e-93	88.50	RING-H2 finger protein ATL72 [Cucumis melo var. makuwa] >TYK04553.1 RING-H2 fing...	[more]
KAG7016045.1	1.8e-91	86.00	RING-H2 finger protein ATL72, partial [Cucurbita argyrosperma subsp. argyrosperm...	[more]
XP_023550807.1	1.8e-91	86.00	RING-H2 finger protein ATL74-like [Cucurbita pepo subsp. pepo] >XP_023550808.1 R...	[more]
XP_022938777.1	5.2e-91	85.50	RING-H2 finger protein ATL74-like [Cucurbita moschata]	[more]

BLAST of MS003354 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SG96	2.9e-44	61.35	RING-H2 finger protein ATL72 OS=Arabidopsis thaliana OX=3702 GN=ATL72 PE=2 SV=1	[more]
Q9FLC6	6.1e-42	62.04	RING-H2 finger protein ATL73 OS=Arabidopsis thaliana OX=3702 GN=ATL73 PE=2 SV=1	[more]
Q9LZV8	3.9e-41	55.84	RING-H2 finger protein ATL74 OS=Arabidopsis thaliana OX=3702 GN=ATL74 PE=2 SV=1	[more]
Q6NQG7	3.8e-36	46.71	RING-H2 finger protein ATL78 OS=Arabidopsis thaliana OX=3702 GN=ATL78 PE=2 SV=1	[more]
P0C034	9.1e-30	47.76	RING-H2 finger protein ATL10 OS=Arabidopsis thaliana OX=3702 GN=ATL10 PE=2 SV=1	[more]

BLAST of MS003354 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1BZW4	1.7e-100	98.42	RING-H2 finger protein ATL74 OS=Momordica charantia OX=3673 GN=LOC111006761 PE=4...	[more]
A0A5D3BXT6	2.7e-93	88.50	RING-H2 finger protein ATL72 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca...	[more]
A0A0A0KAH2	1.3e-92	88.00	RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G014550 P...	[more]
A0A6J1FF16	2.5e-91	85.50	RING-H2 finger protein ATL74-like OS=Cucurbita moschata OX=3662 GN=LOC111444892 ...	[more]
A0A6J1JVF4	3.3e-91	85.50	RING-H2 finger protein ATL74-like OS=Cucurbita maxima OX=3661 GN=LOC111489233 PE...	[more]

BLAST of MS003354 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G10910.1	2.1e-45	61.35	RING/U-box superfamily protein	[more]
AT5G05280.1	4.3e-43	62.04	RING/U-box superfamily protein	[more]
AT5G01880.1	2.8e-42	55.84	RING/U-box superfamily protein	[more]
AT1G49230.1	2.7e-37	46.71	RING/U-box superfamily protein	[more]
AT1G49220.1	6.4e-31	47.76	RING/U-box superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 117..158 e-value: 1.1E-7 score: 41.5
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 116..159 e-value: 5.0E-13 score: 49.2
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 117..159 score: 12.238477
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 78..164 e-value: 4.9E-23 score: 82.6
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 178..200
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..33
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..15
None	No IPR available	PANTHER	PTHR45798:SF44	RING-H2 FINGER PROTEIN ATL74	coord: 11..183
None	No IPR available	PANTHER	PTHR45798	RING-H2 FINGER PROTEIN ATL61-RELATED-RELATED	coord: 11..183
None	No IPR available	CDD	cd16486	mRING-H2-C3H2C2D_ZSWM2	coord: 117..159 e-value: 1.65538E-16 score: 68.0221
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 110..165

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS003354.1	MS003354.1	mRNA