MS003348 (gene) Bitter gourd (TR) v1

Overview


Name	MS003348
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	transcription elongation factor TFIIS
Location	scaffold234: 2276034 .. 2277481 (-)
RNA-Seq Expression	MS003348
Synteny	MS003348

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGAATGAGCTGGTGGAATTGTTTGAAGCGGCGAAGAAGGCTGCAGATGCCGCAGCAGCTCCATCAAATGATGGGGGAGCGGAGGAAAGTCGTTGCCTAGATGCCTTACGGCAGCTCAAGAAATTTCCTGTTACTTATCAAATCCTTGTTTCCACTCAGGTTTTCTTCTCATTAGACTTGATAATCGTTTCTTTTTCTAGACGACTAGTTCATAATATCATGCATGATTCTTATTATGAATTAAGCCAAAATTTCATCGGTTCCATAACATGCTGAGTTCACTTTATTTCATCTGTTATTCGAGATTGTTCACTTAATGATATCAATTCATTTGTGACCATTCCAAGTTTTTGGAACATAGGTCTGGAACCATAGTTCTTGATATGTTTTCTTTTCTGGAATTTCTTTAATCCCTGTTCCGGGTAATTGAGGTCCATTTGCCTGTGTTGATGACTCCAATATATTGCATTCTGTAGGTCGGGAAGCGCCTTCGGCACCTCACTAAACACCCCAAGAAGAAAATACAGGACTATGCATCTGACCTGATTGAGATGTGGAAGGAAATAGTTATCAAGGAGACTAACAAGAATAAGAAAAATGGAAACGGCAATTGTAAAGAGTCGCCTAAAATTGGTTCTCCCAGTGCAGATTCTGTCAAGGTGGAGAAGTTTCAAAAATCATCTTCCATGAAGGTTGAGAGAGTTTCCAAGGTTGAGCAGTCCGATAGGAATGGCACCACGAGCTCTGTAAAATATTCTCGATCAGAAAGTGCGATCTCGGAGAAGAACATTGTCAAGGTTGAGAAAACTGATTCATTGGAGAAGGTCAAGGCTGAAAGGGTGGTTAAAGAAGAGAAGAAAATCTCAATAGATAAGAGACCATCAAGTGGCACTGCTGCACCTCCAAAGCTTACATCAATGATCAAATCAAAAGATGCTGCTCGAGACAAAATAAGAGAACTTCTTTGTGAAGCTTTCTCCAAGGTTCCTGGCGAGGCCGATGAAGATGTTATAGATGAGGTCAATGCAAGCGACCCCATCCGTGTTGCTGTTTCTGTAGAATCCGTGATGTTTGAGAATTGGGGAGGTTCTACCGGGGCACAGAAGGCCAAGTACAGATCTATAATGTTCAACCTCAAGGATCCCAAGAACCCAGATTTCCGGAGAAACGTTCTTGTTGGACATATCAAGCCCGAAAGGCTAATTAATATGAGCACAGCCGAGATGGCAAGCGATCAGAGGAAACGTGAGAATGAAGAGATTGAACAAAAAGCTTTATTTGACTGTGAGCGTGGAGGAGCTCCGAAAGCTACTACCGATCAATTCAAATGTGGTCGATGTGGTCAACGCAAGACGACCTACTATCAATTGCAGACGCGGAGTGCCGATGAACCTATGACTACATTTGTAACTTGTGTAAACTGCAATAACCATTGGAAGTTCTGT

mRNA sequence

ATGGAGAATGAGCTGGTGGAATTGTTTGAAGCGGCGAAGAAGGCTGCAGATGCCGCAGCAGCTCCATCAAATGATGGGGGAGCGGAGGAAAGTCGTTGCCTAGATGCCTTACGGCAGCTCAAGAAATTTCCTGTTACTTATCAAATCCTTGTTTCCACTCAGGTCGGGAAGCGCCTTCGGCACCTCACTAAACACCCCAAGAAGAAAATACAGGACTATGCATCTGACCTGATTGAGATGTGGAAGGAAATAGTTATCAAGGAGACTAACAAGAATAAGAAAAATGGAAACGGCAATTGTAAAGAGTCGCCTAAAATTGGTTCTCCCAGTGCAGATTCTGTCAAGGTGGAGAAGTTTCAAAAATCATCTTCCATGAAGGTTGAGAGAGTTTCCAAGGTTGAGCAGTCCGATAGGAATGGCACCACGAGCTCTGTAAAATATTCTCGATCAGAAAGTGCGATCTCGGAGAAGAACATTGTCAAGGTTGAGAAAACTGATTCATTGGAGAAGGTCAAGGCTGAAAGGGTGGTTAAAGAAGAGAAGAAAATCTCAATAGATAAGAGACCATCAAGTGGCACTGCTGCACCTCCAAAGCTTACATCAATGATCAAATCAAAAGATGCTGCTCGAGACAAAATAAGAGAACTTCTTTGTGAAGCTTTCTCCAAGGTTCCTGGCGAGGCCGATGAAGATGTTATAGATGAGGTCAATGCAAGCGACCCCATCCGTGTTGCTGTTTCTGTAGAATCCGTGATGTTTGAGAATTGGGGAGGTTCTACCGGGGCACAGAAGGCCAAGTACAGATCTATAATGTTCAACCTCAAGGATCCCAAGAACCCAGATTTCCGGAGAAACGTTCTTGTTGGACATATCAAGCCCGAAAGGCTAATTAATATGAGCACAGCCGAGATGGCAAGCGATCAGAGGAAACGTGAGAATGAAGAGATTGAACAAAAAGCTTTATTTGACTGTGAGCGTGGAGGAGCTCCGAAAGCTACTACCGATCAATTCAAATGTGGTCGATGTGGTCAACGCAAGACGACCTACTATCAATTGCAGACGCGGAGTGCCGATGAACCTATGACTACATTTGTAACTTGTGTAAACTGCAATAACCATTGGAAGTTCTGT

Coding sequence (CDS)

ATGGAGAATGAGCTGGTGGAATTGTTTGAAGCGGCGAAGAAGGCTGCAGATGCCGCAGCAGCTCCATCAAATGATGGGGGAGCGGAGGAAAGTCGTTGCCTAGATGCCTTACGGCAGCTCAAGAAATTTCCTGTTACTTATCAAATCCTTGTTTCCACTCAGGTCGGGAAGCGCCTTCGGCACCTCACTAAACACCCCAAGAAGAAAATACAGGACTATGCATCTGACCTGATTGAGATGTGGAAGGAAATAGTTATCAAGGAGACTAACAAGAATAAGAAAAATGGAAACGGCAATTGTAAAGAGTCGCCTAAAATTGGTTCTCCCAGTGCAGATTCTGTCAAGGTGGAGAAGTTTCAAAAATCATCTTCCATGAAGGTTGAGAGAGTTTCCAAGGTTGAGCAGTCCGATAGGAATGGCACCACGAGCTCTGTAAAATATTCTCGATCAGAAAGTGCGATCTCGGAGAAGAACATTGTCAAGGTTGAGAAAACTGATTCATTGGAGAAGGTCAAGGCTGAAAGGGTGGTTAAAGAAGAGAAGAAAATCTCAATAGATAAGAGACCATCAAGTGGCACTGCTGCACCTCCAAAGCTTACATCAATGATCAAATCAAAAGATGCTGCTCGAGACAAAATAAGAGAACTTCTTTGTGAAGCTTTCTCCAAGGTTCCTGGCGAGGCCGATGAAGATGTTATAGATGAGGTCAATGCAAGCGACCCCATCCGTGTTGCTGTTTCTGTAGAATCCGTGATGTTTGAGAATTGGGGAGGTTCTACCGGGGCACAGAAGGCCAAGTACAGATCTATAATGTTCAACCTCAAGGATCCCAAGAACCCAGATTTCCGGAGAAACGTTCTTGTTGGACATATCAAGCCCGAAAGGCTAATTAATATGAGCACAGCCGAGATGGCAAGCGATCAGAGGAAACGTGAGAATGAAGAGATTGAACAAAAAGCTTTATTTGACTGTGAGCGTGGAGGAGCTCCGAAAGCTACTACCGATCAATTCAAATGTGGTCGATGTGGTCAACGCAAGACGACCTACTATCAATTGCAGACGCGGAGTGCCGATGAACCTATGACTACATTTGTAACTTGTGTAAACTGCAATAACCATTGGAAGTTCTGT

Protein sequence

MENELVELFEAAKKAADAAAAPSNDGGAEESRCLDALRQLKKFPVTYQILVSTQVGKRLRHLTKHPKKKIQDYASDLIEMWKEIVIKETNKNKKNGNGNCKESPKIGSPSADSVKVEKFQKSSSMKVERVSKVEQSDRNGTTSSVKYSRSESAISEKNIVKVEKTDSLEKVKAERVVKEEKKISIDKRPSSGTAAPPKLTSMIKSKDAARDKIRELLCEAFSKVPGEADEDVIDEVNASDPIRVAVSVESVMFENWGGSTGAQKAKYRSIMFNLKDPKNPDFRRNVLVGHIKPERLINMSTAEMASDQRKRENEEIEQKALFDCERGGAPKATTDQFKCGRCGQRKTTYYQLQTRSADEPMTTFVTCVNCNNHWKFC

Homology

[Close]

BLAST of MS003348 vs. NCBI nr
Match: XP_022134049.1 (transcription elongation factor TFIIS [Momordica charantia] >XP_022134050.1 transcription elongation factor TFIIS [Momordica charantia])

HSP 1 Score: 721.5 bits (1861), Expect = 3.9e-204
Identity = 374/377 (99.20%), Postives = 376/377 (99.73%), Query Frame = 0


Query: 1   MENELVELFEAAKKAADAAAAPSNDGGAEESRCLDALRQLKKFPVTYQILVSTQVGKRLR 60
           MENELVELFEAAKKAADAAAAPSNDGGAEESRCLDALRQLKKFPVTYQILVSTQVGKRLR
Sbjct: 1   MENELVELFEAAKKAADAAAAPSNDGGAEESRCLDALRQLKKFPVTYQILVSTQVGKRLR 60

Query: 61  HLTKHPKKKIQDYASDLIEMWKEIVIKETNKNKKNGNGNCKESPKIGSPSADSVKVEKFQ 120
           HLTKHPKKKIQDYASDLIEMWKEIVIKETNKNKKNGNGNCKESPKIGSPSA+SVKVEKFQ
Sbjct: 61  HLTKHPKKKIQDYASDLIEMWKEIVIKETNKNKKNGNGNCKESPKIGSPSAESVKVEKFQ 120

Query: 121 KSSSMKVERVSKVEQSDRNGTTSSVKYSRSESAISEKNIVKVEKTDSLEKVKAERVVKEE 180
           KSSSMKVERVSKVEQSDRNGTTSSVKYSRSES ISEKNIVKVEKTDSLEKVKAERVVKEE
Sbjct: 121 KSSSMKVERVSKVEQSDRNGTTSSVKYSRSESVISEKNIVKVEKTDSLEKVKAERVVKEE 180

Query: 181 KKISIDKRPSSGTAAPPKLTSMIKSKDAARDKIRELLCEAFSKVPGEADEDVIDEVNASD 240
           KKISIDKRPSSGTAAPPKLTSMIKSKDAARDKIRELLCEAFSKVPGEADEDVIDEVNASD
Sbjct: 181 KKISIDKRPSSGTAAPPKLTSMIKSKDAARDKIRELLCEAFSKVPGEADEDVIDEVNASD 240

Query: 241 PIRVAVSVESVMFENWGGSTGAQKAKYRSIMFNLKDPKNPDFRRNVLVGHIKPERLINMS 300
           P+RVAVSVESVMFENWGGSTGAQKAKYRSIMFNLKDPKNPDFRRNVLVGHIKPERLINMS
Sbjct: 241 PVRVAVSVESVMFENWGGSTGAQKAKYRSIMFNLKDPKNPDFRRNVLVGHIKPERLINMS 300

Query: 301 TAEMASDQRKRENEEIEQKALFDCERGGAPKATTDQFKCGRCGQRKTTYYQLQTRSADEP 360
           TAEMASDQRKRENEEIEQKALFDCERGGAPKATTDQFKCGRCGQRKTTYYQLQTRSADEP
Sbjct: 301 TAEMASDQRKRENEEIEQKALFDCERGGAPKATTDQFKCGRCGQRKTTYYQLQTRSADEP 360

Query: 361 MTTFVTCVNCNNHWKFC 378
           MTTFVTCVNCNNHWKFC
Sbjct: 361 MTTFVTCVNCNNHWKFC 377

[Close]

BLAST of MS003348 vs. NCBI nr
Match: XP_038884917.1 (transcription elongation factor TFIIS [Benincasa hispida])

HSP 1 Score: 667.2 bits (1720), Expect = 8.6e-188
Identity = 346/377 (91.78%), Postives = 361/377 (95.76%), Query Frame = 0


Query: 1   MENELVELFEAAKKAADAAAAPSNDGGAEESRCLDALRQLKKFPVTYQILVSTQVGKRLR 60
           MENELVELF+AAKKAADAAAAPSNDGGAEESRCLDALRQLKKFPVTYQILVSTQVGKRLR
Sbjct: 1   MENELVELFQAAKKAADAAAAPSNDGGAEESRCLDALRQLKKFPVTYQILVSTQVGKRLR 60

Query: 61  HLTKHPKKKIQDYASDLIEMWKEIVIKETNKNKKNGNGNCKESPKIGSPSADSVKVEKFQ 120
           HLTKHPKKKIQ+YASDL+EMWKEIVIKETNKNKKNGNGNCK+SPKIGSPSA+SVKVEKFQ
Sbjct: 61  HLTKHPKKKIQEYASDLVEMWKEIVIKETNKNKKNGNGNCKDSPKIGSPSAESVKVEKFQ 120

Query: 121 KSSSMKVERVSKVEQSDRNGTTSSVKYSRSESAISEKNIVKVEKTDSLEKVKAERVVKEE 180
           KSSSMKVERVSKVEQ D+NG T SVKYSR ESA+SE+N VKVEKTDS   VK E  VKEE
Sbjct: 121 KSSSMKVERVSKVEQFDKNGATGSVKYSRLESAVSERNSVKVEKTDS--AVKVEGTVKEE 180

Query: 181 KKISIDKRPSSGTAAPPKLTSMIKSKDAARDKIRELLCEAFSKVPGEADEDVIDEVNASD 240
           ++ISIDK+PS GTAAPPKLTSMIKSKDAARDKIRELL EAFSKVPGEADEDV+DEVNASD
Sbjct: 181 RRISIDKKPSGGTAAPPKLTSMIKSKDAARDKIRELLFEAFSKVPGEADEDVMDEVNASD 240

Query: 241 PIRVAVSVESVMFENWGGSTGAQKAKYRSIMFNLKDPKNPDFRRNVLVGHIKPERLINMS 300
           PIRVAVSVESVMFENWGGSTGAQKAKYRSIMFNLKDPKNPDFRR VL+G IKPER+INMS
Sbjct: 241 PIRVAVSVESVMFENWGGSTGAQKAKYRSIMFNLKDPKNPDFRRKVLLGLIKPERMINMS 300

Query: 301 TAEMASDQRKRENEEIEQKALFDCERGGAPKATTDQFKCGRCGQRKTTYYQLQTRSADEP 360
           TA+MASDQRKRENEEI QKALFDCERGGAPKATTDQFKCGRCGQRKTTYYQLQTRSADEP
Sbjct: 301 TADMASDQRKRENEEIAQKALFDCERGGAPKATTDQFKCGRCGQRKTTYYQLQTRSADEP 360

Query: 361 MTTFVTCVNCNNHWKFC 378
           MTTFVTCVNCNNHWKFC
Sbjct: 361 MTTFVTCVNCNNHWKFC 375

[Close]

BLAST of MS003348 vs. NCBI nr
Match: KAG6578486.1 (Transcription elongation factor TFIIS, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 655.2 bits (1689), Expect = 3.4e-184
Identity = 343/377 (90.98%), Postives = 359/377 (95.23%), Query Frame = 0


Query: 1   MENELVELFEAAKKAADAAAAPSNDGGAEESRCLDALRQLKKFPVTYQILVSTQVGKRLR 60
           MENELVELFEAAKKAADAAAAPSNDGGAEESRCLDALRQLKKFPVTYQILVSTQVGKRLR
Sbjct: 1   MENELVELFEAAKKAADAAAAPSNDGGAEESRCLDALRQLKKFPVTYQILVSTQVGKRLR 60

Query: 61  HLTKHPKKKIQDYASDLIEMWKEIVIKETNKNKKNGNGNCKESPKIGSPSADSVKVEKFQ 120
           HLTKHPKKKIQ+YASDLIE+WKEIVIKETNKNKKNGN +CKESPKIGSPSA+SVKVEKF+
Sbjct: 61  HLTKHPKKKIQNYASDLIEIWKEIVIKETNKNKKNGNIDCKESPKIGSPSAESVKVEKFR 120

Query: 121 KSSSMKVERVSKVEQSDRNGTTSSVKYSRSESAISEKNIVKVEKTDSLEKVKAERVVKEE 180
           KSSSMKVERVS+VEQ DRNG TSS K+SRSES IS KN VKVEKTDS+  VK ER+VKEE
Sbjct: 121 KSSSMKVERVSQVEQFDRNGVTSSAKFSRSESDISGKNSVKVEKTDSV--VKVERIVKEE 180

Query: 181 KKISIDKRPSSGTAAPPKLTSMIKSKDAARDKIRELLCEAFSKVPGEADEDVIDEVNASD 240
           K+ISI+K+PSSG  APPKLTSMIKSKDAARDKIRELL EAFSKVP EADEDV+DEVNASD
Sbjct: 181 KRISIEKKPSSGPDAPPKLTSMIKSKDAARDKIRELLFEAFSKVPSEADEDVMDEVNASD 240

Query: 241 PIRVAVSVESVMFENWGGSTGAQKAKYRSIMFNLKDPKNPDFRRNVLVGHIKPERLINMS 300
           PIRVAVSVESVMFENWGGSTG+QK KYRSIMFNLKDPKNPDFRR VL+G IKPERLINMS
Sbjct: 241 PIRVAVSVESVMFENWGGSTGSQKTKYRSIMFNLKDPKNPDFRRKVLLGLIKPERLINMS 300

Query: 301 TAEMASDQRKRENEEIEQKALFDCERGGAPKATTDQFKCGRCGQRKTTYYQLQTRSADEP 360
           TA+MASDQRKRENEEIEQKALFDCER GAPKATTDQFKCGRCGQRKTTYYQLQTRSADEP
Sbjct: 301 TADMASDQRKRENEEIEQKALFDCERAGAPKATTDQFKCGRCGQRKTTYYQLQTRSADEP 360

Query: 361 MTTFVTCVNCNNHWKFC 378
           MTTFVTCVNCNNHWKFC
Sbjct: 361 MTTFVTCVNCNNHWKFC 375

[Close]

BLAST of MS003348 vs. NCBI nr
Match: KAG7016050.1 (Transcription elongation factor TFIIS, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 652.9 bits (1683), Expect = 1.7e-183
Identity = 342/377 (90.72%), Postives = 358/377 (94.96%), Query Frame = 0


Query: 1   MENELVELFEAAKKAADAAAAPSNDGGAEESRCLDALRQLKKFPVTYQILVSTQVGKRLR 60
           MENELVELFEAAKKAADAAAAPSNDGGAEESRCLDALRQLKKFPVTYQILVSTQVGKRLR
Sbjct: 1   MENELVELFEAAKKAADAAAAPSNDGGAEESRCLDALRQLKKFPVTYQILVSTQVGKRLR 60

Query: 61  HLTKHPKKKIQDYASDLIEMWKEIVIKETNKNKKNGNGNCKESPKIGSPSADSVKVEKFQ 120
           HLTKHPKKKIQ+YASDLIE+WKEIVIKETNKNKKNGN +CKESPKIGSPSA+SVKVEKF+
Sbjct: 61  HLTKHPKKKIQNYASDLIEIWKEIVIKETNKNKKNGNIDCKESPKIGSPSAESVKVEKFR 120

Query: 121 KSSSMKVERVSKVEQSDRNGTTSSVKYSRSESAISEKNIVKVEKTDSLEKVKAERVVKEE 180
           KSSSMKVERVS+VEQ DRNG TSS K+SR ES IS KN VKVEKTDS+  VK ER+VKEE
Sbjct: 121 KSSSMKVERVSQVEQFDRNGVTSSAKFSRLESDISGKNSVKVEKTDSV--VKVERIVKEE 180

Query: 181 KKISIDKRPSSGTAAPPKLTSMIKSKDAARDKIRELLCEAFSKVPGEADEDVIDEVNASD 240
           K+ISI+K+PSSG  APPKLTSMIKSKDAARDKIRELL EAFSKVP EADEDV+DEVNASD
Sbjct: 181 KRISIEKKPSSGPDAPPKLTSMIKSKDAARDKIRELLFEAFSKVPSEADEDVMDEVNASD 240

Query: 241 PIRVAVSVESVMFENWGGSTGAQKAKYRSIMFNLKDPKNPDFRRNVLVGHIKPERLINMS 300
           PIRVAVSVESVMFENWGGSTG+QK KYRSIMFNLKDPKNPDFRR VL+G IKPERLINMS
Sbjct: 241 PIRVAVSVESVMFENWGGSTGSQKTKYRSIMFNLKDPKNPDFRRKVLLGLIKPERLINMS 300

Query: 301 TAEMASDQRKRENEEIEQKALFDCERGGAPKATTDQFKCGRCGQRKTTYYQLQTRSADEP 360
           TA+MASDQRKRENEEIEQKALFDCER GAPKATTDQFKCGRCGQRKTTYYQLQTRSADEP
Sbjct: 301 TADMASDQRKRENEEIEQKALFDCERAGAPKATTDQFKCGRCGQRKTTYYQLQTRSADEP 360

Query: 361 MTTFVTCVNCNNHWKFC 378
           MTTFVTCVNCNNHWKFC
Sbjct: 361 MTTFVTCVNCNNHWKFC 375

[Close]

BLAST of MS003348 vs. NCBI nr
Match: XP_022938772.1 (transcription elongation factor TFIIS-like [Cucurbita moschata])

HSP 1 Score: 652.1 bits (1681), Expect = 2.9e-183
Identity = 341/377 (90.45%), Postives = 359/377 (95.23%), Query Frame = 0


Query: 1   MENELVELFEAAKKAADAAAAPSNDGGAEESRCLDALRQLKKFPVTYQILVSTQVGKRLR 60
           MENELVELFEAAKKAADAAAAPSNDGGAEESRCLDALRQLKKFPVTYQILVSTQVGKRLR
Sbjct: 1   MENELVELFEAAKKAADAAAAPSNDGGAEESRCLDALRQLKKFPVTYQILVSTQVGKRLR 60

Query: 61  HLTKHPKKKIQDYASDLIEMWKEIVIKETNKNKKNGNGNCKESPKIGSPSADSVKVEKFQ 120
           HLTKHPKKKIQ+YASDLIE+WKEIVIK+TNKNKKNGN +C+ESPKIGSPSA+SVKVEKF+
Sbjct: 61  HLTKHPKKKIQNYASDLIEIWKEIVIKDTNKNKKNGNIDCEESPKIGSPSAESVKVEKFR 120

Query: 121 KSSSMKVERVSKVEQSDRNGTTSSVKYSRSESAISEKNIVKVEKTDSLEKVKAERVVKEE 180
           KSSSMKVERVS+VEQ DRNG TSS K+SRSES IS KN VKVEKTDS+  VK ER+VKEE
Sbjct: 121 KSSSMKVERVSQVEQFDRNGVTSSAKFSRSESDISGKNPVKVEKTDSV--VKVERIVKEE 180

Query: 181 KKISIDKRPSSGTAAPPKLTSMIKSKDAARDKIRELLCEAFSKVPGEADEDVIDEVNASD 240
           K+ISI+K+PSSG  APPKLTSMIKSKDAARDKIRELL EAFSKVP EADEDV+DEVNASD
Sbjct: 181 KRISIEKKPSSGPDAPPKLTSMIKSKDAARDKIRELLFEAFSKVPSEADEDVMDEVNASD 240

Query: 241 PIRVAVSVESVMFENWGGSTGAQKAKYRSIMFNLKDPKNPDFRRNVLVGHIKPERLINMS 300
           PIRVAVSVESVMFENWGGSTG+QK KYRSIMFNLKDPKNPDFRR VL+G IKPERLINMS
Sbjct: 241 PIRVAVSVESVMFENWGGSTGSQKTKYRSIMFNLKDPKNPDFRRKVLLGLIKPERLINMS 300

Query: 301 TAEMASDQRKRENEEIEQKALFDCERGGAPKATTDQFKCGRCGQRKTTYYQLQTRSADEP 360
           TA+MASDQRKRENEEIEQKALFDCER GAPKATTDQFKCGRCGQRKTTYYQLQTRSADEP
Sbjct: 301 TADMASDQRKRENEEIEQKALFDCERAGAPKATTDQFKCGRCGQRKTTYYQLQTRSADEP 360

Query: 361 MTTFVTCVNCNNHWKFC 378
           MTTFVTCVNCNNHWKFC
Sbjct: 361 MTTFVTCVNCNNHWKFC 375

[Close]

BLAST of MS003348 vs. ExPASy Swiss-Prot
Match: Q9ZVH8 (Transcription elongation factor TFIIS OS=Arabidopsis thaliana OX=3702 GN=TFIIS PE=1 SV=1)

HSP 1 Score: 387.9 bits (995), Expect = 1.3e-106
Identity = 221/384 (57.55%), Postives = 273/384 (71.09%), Query Frame = 0


Query: 1   MENELVELFEAAKKAADAAAAPS-NDGGAEESRCLDALRQLKKFPVTYQILVSTQVGKRL 60
           ME++L++LFE AKKAADAAA       G E S+C+DAL+QLKKFPVTY  LV+TQVGK+L
Sbjct: 1   MESDLIDLFEGAKKAADAAALDGVTSSGPEVSQCIDALKQLKKFPVTYDTLVATQVGKKL 60

Query: 61  RHLTKHPKKKIQDYASDLIEMWKEIVIKETNKNKK-NGNGNCKESP----KIGSPSADS- 120
           R L KHP + I+  A+DL+E+WK++VI+ET K KK  G   CKE+      +  PS  + 
Sbjct: 61  RSLAKHPVEDIKSVATDLLEIWKKVVIEETAKAKKTEGTNGCKEAKVNKMDVEKPSNPAP 120

Query: 121 VKVEKFQKSSSMKVERVSKVEQSDRNGTTSSVKYSRSESAISEKNIVKVEKTDSLEKVKA 180
           VKV+K Q+  S K  +V + E    N   + VK  R    I   N  K++     + VK 
Sbjct: 121 VKVQKLQRGDSAKSIKVERKEPD--NKVVTGVKIERKVPDIKVTNGTKIDYRG--QAVKD 180

Query: 181 ERVVKEEKKISIDKRPSSGTAAPPKLTSMIKSKDAARDKIRELLCEAFSKVPGEADEDVI 240
           E+V K+ +  S  K P+    APPKLT+M+K  D  RDKIRELL EA  +V GEAD+   
Sbjct: 181 EKVSKDNQ--SSMKAPAKAANAPPKLTAMLKCNDPVRDKIRELLVEALCRVAGEADDYER 240

Query: 241 DEVNASDPIRVAVSVESVMFENWGGSTGAQKAKYRSIMFNLKDPKNPDFRRNVLVGHIKP 300
           + VNASDP+RVAVSVES+MFE  G STGAQK KYRSIMFNL+D  NPD RR VL G I P
Sbjct: 241 ESVNASDPLRVAVSVESLMFEKLGRSTGAQKLKYRSIMFNLRDSNNPDLRRRVLTGEISP 300

Query: 301 ERLINMSTAEMASDQRKRENEEIEQKALFDCERGGAPKATTDQFKCGRCGQRKTTYYQLQ 360
           E+LI +S  +MASD+RK+EN +I++KALFDCERG A KA+TDQFKCGRCGQRK TYYQ+Q
Sbjct: 301 EKLITLSAEDMASDKRKQENNQIKEKALFDCERGLAAKASTDQFKCGRCGQRKCTYYQMQ 360

Query: 361 TRSADEPMTTFVTCVNCNNHWKFC 378
           TRSADEPMTT+VTCVNC+NHWKFC
Sbjct: 361 TRSADEPMTTYVTCVNCDNHWKFC 378

[Close]

BLAST of MS003348 vs. ExPASy Swiss-Prot
Match: P20232 (Transcription elongation factor S-II OS=Drosophila melanogaster OX=7227 GN=TfIIS PE=2 SV=1)

HSP 1 Score: 133.3 bits (334), Expect = 5.9e-30
Identity = 111/377 (29.44%), Postives = 160/377 (42.44%), Query Frame = 0


Query: 7   ELFEAAKKAADAAAAPSNDGGAEESRCLDALRQLKKFPVTYQILVSTQVGKRLRHLTKHP 66
           E+F   KK +  A+      G  + + LD L+ L+   +   IL  T++G  +  L K  
Sbjct: 6   EVFRIQKKMSKMAS-----DGTGQDQALDLLKALQTLNINLDILTKTRIGMTVNELRKSS 65

Query: 67  K-KKIQDYASDLIEMWKEIVIK-----ETNKNKKNGNGNCKESPKIGSPSADSVKVEKFQ 126
           K  ++   A  LI+ WK  +         N + K G+ N   + K  S +  S  +    
Sbjct: 66  KDDEVIALAKTLIKNWKRFLASPAPTTPNNSSAKEGSSNNSSASKSTSAAKSSSSISGKD 125

Query: 127 KSSSMKVERVSKVEQSDRNGTTSSVKYSRSESAISEKNIVKVEKTDSLEKVKAERVVKEE 186
           KSSS      S  +  ++ G+TSS + S                                
Sbjct: 126 KSSSS-----SSSKDKEKKGSTSSSQTS-------------------------------- 185

Query: 187 KKISIDKRPSSGTAAPPKLTSMIKSKDAARDKIRELLCEAFSKVPGEADEDVIDEVNASD 246
                   PS G              DA R K RE+L  A     GE  E         +
Sbjct: 186 -------FPSGGMT------------DAVRIKCREMLATALK--IGEVPE------GCGE 245

Query: 247 PIRVAVSVESVMFENWGGSTGAQKAKYRSIMFNLKDPKNPDFRRNVLVGHIKPERLINMS 306
           P  +A  +E  ++  +  +    K + RS + NLKDPKNP  R N + G +  ++L  M+
Sbjct: 246 PEEMAAELEDAIYSEFNNTDMKYKNRIRSRVANLKDPKNPGLRGNFMCGAVTAKQLAKMT 305

Query: 307 TAEMASDQRKRENEEIEQKALFDCERGGAPKATTDQFKCGRCGQRKTTYYQLQTRSADEP 366
             EMASD+ K+  E+  ++A+ D +        TD  KC +C +R  TY QLQTRSADEP
Sbjct: 306 PEEMASDEMKKLREKFVKEAINDAQLATVQGTKTDLLKCAKCKKRNCTYNQLQTRSADEP 313

Query: 367 MTTFVTCVNCNNHWKFC 378
           MTTFV C  C N WKFC
Sbjct: 366 MTTFVMCNECGNRWKFC 313

[Close]

BLAST of MS003348 vs. ExPASy Swiss-Prot
Match: Q2KI09 (Transcription elongation factor A protein 3 OS=Bos taurus OX=9913 GN=TCEA3 PE=2 SV=1)

HSP 1 Score: 132.1 bits (331), Expect = 1.3e-29
Identity = 116/373 (31.10%), Postives = 175/373 (46.92%), Query Frame = 0


Query: 7   ELFEAAKKAADAAAAPSNDGGAEESRCLDALRQLKKFPVTYQILVSTQVGKRLRHLTKH- 66
           EL   AKK     A    +G       LD L++L  + ++ Q+L +T++G  +  + KH 
Sbjct: 6   ELLRIAKKLEKMVARKKTEG------ALDLLKKLGSWQMSIQLLQTTRIGVAVNGVRKHC 65

Query: 67  PKKKIQDYASDLIEMWKEIVIKETNKNKKNGNGNCKESPKIGSPSADSVKVEKFQKSSSM 126
             K++   A  LI  WK+++        + G    K   K        +    ++  +S+
Sbjct: 66  SNKEVVALAKVLIRNWKQLLDSPATPKGEKGEERVKAKKK-----EKGLDCSDWKPETSL 125

Query: 127 KVERVSKVEQ-SDRNGTTSSVKYSRSESAISEKNIVKVEKTDSLEKVKAERVVKEEKKIS 186
              R  +VE+  DR  +  S       SA S      +E+++S  K KAE         S
Sbjct: 126 SPPRKKRVEEPKDRRDSVDS-----KSSATSSPKRPSMERSNS-SKSKAETPRTPSSPSS 185

Query: 187 IDKRPSSGTAAPPKLTSMIKSKDAARDKIRELLCEAFSKVPGEADEDVIDEVNASDPIRV 246
               PS    AP  LT      D+ RDK  E+L  A      +AD+D  D     D  ++
Sbjct: 186 PTFAPSVCLLAPCYLTG-----DSVRDKCVEMLSAAL-----KADDDYKDYGVNCD--KM 245

Query: 247 AVSVESVMFENWGGSTGAQKAKYRSIMFNLKDPKNPDFRRNVLVGHIKPERLINMSTAEM 306
           A  +E  +++    +    + + RS + NLKDP+NP  RRNVL G I    +  M+  EM
Sbjct: 246 ASEIEDHIYQELKSTDMKYRNRVRSRISNLKDPRNPGLRRNVLSGAISAGLIAKMTAEEM 305

Query: 307 ASDQRKRENEEIEQKALFDCERGGAPKATTDQFKCGRCGQRKTTYYQLQTRSADEPMTTF 366
           ASD+ +     + Q+A+ + +       TTD F+C +C ++  TY Q+QTRSADEPMTTF
Sbjct: 306 ASDELRELRNAMTQEAIREHQMAKTGGTTTDLFQCSKCKKKNCTYNQVQTRSADEPMTTF 349

Query: 367 VTCVNCNNHWKFC 378
           V C  C N WKFC
Sbjct: 366 VLCNECGNRWKFC 349

[Close]

BLAST of MS003348 vs. ExPASy Swiss-Prot
Match: P23881 (Transcription elongation factor A protein 3 OS=Mus musculus OX=10090 GN=Tcea3 PE=1 SV=3)

HSP 1 Score: 129.8 bits (325), Expect = 6.5e-29
Identity = 116/379 (30.61%), Postives = 175/379 (46.17%), Query Frame = 0


Query: 7   ELFEAAKKAADAAAAPSNDGGAEESRCLDALRQLKKFPVTYQILVSTQVGKRLRHLTKH- 66
           EL   AKK     +    +G       LD L++L    ++ Q+L +T++G  +  + KH 
Sbjct: 6   ELLRIAKKLEKMVSRKKTEG------ALDLLKKLNSCQMSIQLLQTTRIGVAVNGVRKHC 65

Query: 67  PKKKIQDYASDLIEMWKEIV-IKETNKNKKNGNGNCKESPKIG----SPSADSVKVEKFQ 126
             K++   A  LI+ WK ++    T K ++      K+   +G     P A      K  
Sbjct: 66  SDKEVVSLAKVLIKNWKRLLDSPRTTKGEREEREKAKKEKGLGCSDWKPEAGLSPPRK-- 125

Query: 127 KSSSMKVERVSKVEQSDRNGTTSSVKYSRSESAISEKNIVKVEKTDSLEKVKAERVVKEE 186
           K       R   V+   R+ TTSS K    E + S K+ V+   T S             
Sbjct: 126 KGGGEPKTRRDSVD--SRSSTTSSPKRPSLERSNSSKSKVETPTTPS------------- 185

Query: 187 KKISIDKRPSSGTAAPP--KLTSMIKSKDAARDKIRELLCEAFSKVPGEADEDVIDEVNA 246
                   PS+ T AP    L     + D+ RDK  E+L  A      +A+++  D    
Sbjct: 186 -------SPSTPTFAPAVCLLAPCYLTGDSVRDKCVEMLSAAL-----KAEDNFKDYGVN 245

Query: 247 SDPIRVAVSVESVMFENWGGSTGAQKAKYRSIMFNLKDPKNPDFRRNVLVGHIKPERLIN 306
            D  ++A  +E  +++    +    + + RS + NLKDP+NP  RRNVL G I PE +  
Sbjct: 246 CD--KLASEIEDHIYQELKSTDMKYRNRVRSRISNLKDPRNPGLRRNVLSGAISPELIAK 305

Query: 307 MSTAEMASDQRKRENEEIEQKALFDCERGGAPKATTDQFKCGRCGQRKTTYYQLQTRSAD 366
           M+  EMASD+ +     + Q+A+ + +       TTD  +C +C ++  TY Q+QTRSAD
Sbjct: 306 MTAEEMASDELRELRNAMTQEAIREHQMAKTGGTTTDLLRCSKCKKKNCTYNQVQTRSAD 347

Query: 367 EPMTTFVTCVNCNNHWKFC 378
           EPMTTFV C  C N WKFC
Sbjct: 366 EPMTTFVLCNECGNRWKFC 347

[Close]

BLAST of MS003348 vs. ExPASy Swiss-Prot
Match: P10711 (Transcription elongation factor A protein 1 OS=Mus musculus OX=10090 GN=Tcea1 PE=1 SV=2)

HSP 1 Score: 127.5 bits (319), Expect = 3.2e-28
Identity = 109/379 (28.76%), Postives = 169/379 (44.59%), Query Frame = 0


Query: 1   MENELVELFEAAKKAADAAAAPSNDGGAEESRCLDALRQLKKFPVTYQILVSTQVGKRLR 60
           ME+E+V +     K  D      N  GA     LD L++LK  P+T ++L ST++G  + 
Sbjct: 1   MEDEVVRI----AKKMDKMVQKKNAAGA-----LDLLKELKNIPMTLELLQSTRIGMSVN 60

Query: 61  HLTKH-PKKKIQDYASDLIEMWKEIVIKETNKNKKNGNGNCKESPKIGSPSADSVKVEKF 120
            L K    +++   A  LI+ WK++                     +  PS D    EK 
Sbjct: 61  ALRKQSTDEEVTSLAKSLIKSWKKL---------------------LDGPSTDKDPEEK- 120

Query: 121 QKSSSMKVERVSKVEQSDRNGTTSSVKYSRSESAISEKNIVKV-EKTDSLEKVKAERVVK 180
                                        + E AIS +N  +  E++ S   V +    +
Sbjct: 121 -----------------------------KKEPAISSQNSPEAREESSSSSNVSS----R 180

Query: 181 EEKKISIDKRPSSGTAAPPKLTSMIKSKDAARDKIRELLCEAFSKVPGEADEDVIDEVNA 240
           +++  + D   SS   AP        + D+ R K RE+L  A         +D +     
Sbjct: 181 KDETNARDTYVSSFPRAP-------STSDSVRLKCREMLAAAL-----RTGDDYV--AIG 240

Query: 241 SDPIRVAVSVESVMFENWGGSTGAQKAKYRSIMFNLKDPKNPDFRRNVLVGHIKPERLIN 300
           +D   +   +E  +++    +    K + RS + NLKD KNP+ R+NVL G+I P+    
Sbjct: 241 ADEEELGSQIEEAIYQEIRNTDMKYKNRVRSRISNLKDAKNPNLRKNVLCGNIPPDLFAR 300

Query: 301 MSTAEMASDQRKRENEEIEQKALFDCERGGAPKATTDQFKCGRCGQRKTTYYQLQTRSAD 360
           M+  EMASD+ K   + + ++A+ + +        TD F CG+C ++  TY Q+QTRSAD
Sbjct: 301 MTAEEMASDELKEMRKNLTKEAIREHQMAKTGGTQTDLFTCGKCKKKNCTYTQVQTRSAD 301

Query: 361 EPMTTFVTCVNCNNHWKFC 378
           EPMTTFV C  C N WKFC
Sbjct: 361 EPMTTFVVCNECGNRWKFC 301

[Close]

BLAST of MS003348 vs. ExPASy TrEMBL
Match: A0A6J1BWY6 (transcription elongation factor TFIIS OS=Momordica charantia OX=3673 GN=LOC111006416 PE=4 SV=1)

HSP 1 Score: 721.5 bits (1861), Expect = 1.9e-204
Identity = 374/377 (99.20%), Postives = 376/377 (99.73%), Query Frame = 0


Query: 1   MENELVELFEAAKKAADAAAAPSNDGGAEESRCLDALRQLKKFPVTYQILVSTQVGKRLR 60
           MENELVELFEAAKKAADAAAAPSNDGGAEESRCLDALRQLKKFPVTYQILVSTQVGKRLR
Sbjct: 1   MENELVELFEAAKKAADAAAAPSNDGGAEESRCLDALRQLKKFPVTYQILVSTQVGKRLR 60

Query: 61  HLTKHPKKKIQDYASDLIEMWKEIVIKETNKNKKNGNGNCKESPKIGSPSADSVKVEKFQ 120
           HLTKHPKKKIQDYASDLIEMWKEIVIKETNKNKKNGNGNCKESPKIGSPSA+SVKVEKFQ
Sbjct: 61  HLTKHPKKKIQDYASDLIEMWKEIVIKETNKNKKNGNGNCKESPKIGSPSAESVKVEKFQ 120

Query: 121 KSSSMKVERVSKVEQSDRNGTTSSVKYSRSESAISEKNIVKVEKTDSLEKVKAERVVKEE 180
           KSSSMKVERVSKVEQSDRNGTTSSVKYSRSES ISEKNIVKVEKTDSLEKVKAERVVKEE
Sbjct: 121 KSSSMKVERVSKVEQSDRNGTTSSVKYSRSESVISEKNIVKVEKTDSLEKVKAERVVKEE 180

Query: 181 KKISIDKRPSSGTAAPPKLTSMIKSKDAARDKIRELLCEAFSKVPGEADEDVIDEVNASD 240
           KKISIDKRPSSGTAAPPKLTSMIKSKDAARDKIRELLCEAFSKVPGEADEDVIDEVNASD
Sbjct: 181 KKISIDKRPSSGTAAPPKLTSMIKSKDAARDKIRELLCEAFSKVPGEADEDVIDEVNASD 240

Query: 241 PIRVAVSVESVMFENWGGSTGAQKAKYRSIMFNLKDPKNPDFRRNVLVGHIKPERLINMS 300
           P+RVAVSVESVMFENWGGSTGAQKAKYRSIMFNLKDPKNPDFRRNVLVGHIKPERLINMS
Sbjct: 241 PVRVAVSVESVMFENWGGSTGAQKAKYRSIMFNLKDPKNPDFRRNVLVGHIKPERLINMS 300

Query: 301 TAEMASDQRKRENEEIEQKALFDCERGGAPKATTDQFKCGRCGQRKTTYYQLQTRSADEP 360
           TAEMASDQRKRENEEIEQKALFDCERGGAPKATTDQFKCGRCGQRKTTYYQLQTRSADEP
Sbjct: 301 TAEMASDQRKRENEEIEQKALFDCERGGAPKATTDQFKCGRCGQRKTTYYQLQTRSADEP 360

Query: 361 MTTFVTCVNCNNHWKFC 378
           MTTFVTCVNCNNHWKFC
Sbjct: 361 MTTFVTCVNCNNHWKFC 377

[Close]

BLAST of MS003348 vs. ExPASy TrEMBL
Match: A0A6J1FF11 (transcription elongation factor TFIIS-like OS=Cucurbita moschata OX=3662 GN=LOC111444888 PE=4 SV=1)

HSP 1 Score: 652.1 bits (1681), Expect = 1.4e-183
Identity = 341/377 (90.45%), Postives = 359/377 (95.23%), Query Frame = 0


Query: 1   MENELVELFEAAKKAADAAAAPSNDGGAEESRCLDALRQLKKFPVTYQILVSTQVGKRLR 60
           MENELVELFEAAKKAADAAAAPSNDGGAEESRCLDALRQLKKFPVTYQILVSTQVGKRLR
Sbjct: 1   MENELVELFEAAKKAADAAAAPSNDGGAEESRCLDALRQLKKFPVTYQILVSTQVGKRLR 60

Query: 61  HLTKHPKKKIQDYASDLIEMWKEIVIKETNKNKKNGNGNCKESPKIGSPSADSVKVEKFQ 120
           HLTKHPKKKIQ+YASDLIE+WKEIVIK+TNKNKKNGN +C+ESPKIGSPSA+SVKVEKF+
Sbjct: 61  HLTKHPKKKIQNYASDLIEIWKEIVIKDTNKNKKNGNIDCEESPKIGSPSAESVKVEKFR 120

Query: 121 KSSSMKVERVSKVEQSDRNGTTSSVKYSRSESAISEKNIVKVEKTDSLEKVKAERVVKEE 180
           KSSSMKVERVS+VEQ DRNG TSS K+SRSES IS KN VKVEKTDS+  VK ER+VKEE
Sbjct: 121 KSSSMKVERVSQVEQFDRNGVTSSAKFSRSESDISGKNPVKVEKTDSV--VKVERIVKEE 180

Query: 181 KKISIDKRPSSGTAAPPKLTSMIKSKDAARDKIRELLCEAFSKVPGEADEDVIDEVNASD 240
           K+ISI+K+PSSG  APPKLTSMIKSKDAARDKIRELL EAFSKVP EADEDV+DEVNASD
Sbjct: 181 KRISIEKKPSSGPDAPPKLTSMIKSKDAARDKIRELLFEAFSKVPSEADEDVMDEVNASD 240

Query: 241 PIRVAVSVESVMFENWGGSTGAQKAKYRSIMFNLKDPKNPDFRRNVLVGHIKPERLINMS 300
           PIRVAVSVESVMFENWGGSTG+QK KYRSIMFNLKDPKNPDFRR VL+G IKPERLINMS
Sbjct: 241 PIRVAVSVESVMFENWGGSTGSQKTKYRSIMFNLKDPKNPDFRRKVLLGLIKPERLINMS 300

Query: 301 TAEMASDQRKRENEEIEQKALFDCERGGAPKATTDQFKCGRCGQRKTTYYQLQTRSADEP 360
           TA+MASDQRKRENEEIEQKALFDCER GAPKATTDQFKCGRCGQRKTTYYQLQTRSADEP
Sbjct: 301 TADMASDQRKRENEEIEQKALFDCERAGAPKATTDQFKCGRCGQRKTTYYQLQTRSADEP 360

Query: 361 MTTFVTCVNCNNHWKFC 378
           MTTFVTCVNCNNHWKFC
Sbjct: 361 MTTFVTCVNCNNHWKFC 375

[Close]

BLAST of MS003348 vs. ExPASy TrEMBL
Match: A0A0A0KDH9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G014610 PE=4 SV=1)

HSP 1 Score: 649.8 bits (1675), Expect = 6.9e-183
Identity = 342/377 (90.72%), Postives = 356/377 (94.43%), Query Frame = 0


Query: 1   MENELVELFEAAKKAADAAAAPSNDGGAEESRCLDALRQLKKFPVTYQILVSTQVGKRLR 60
           MENELVELFEAAKKAADAAAAPSNDGGAEESRCLDALRQLKKFPVTYQILVSTQVGKRLR
Sbjct: 1   MENELVELFEAAKKAADAAAAPSNDGGAEESRCLDALRQLKKFPVTYQILVSTQVGKRLR 60

Query: 61  HLTKHPKKKIQDYASDLIEMWKEIVIKETNKNKKNGNGNCKESPKIGSPSADSVKVEKFQ 120
           HLTKHPKKKIQ++ASDLIEMWKEIVIKETNKNKKNGN + K+SPKIGSPSA+SVKVEKFQ
Sbjct: 61  HLTKHPKKKIQEHASDLIEMWKEIVIKETNKNKKNGNASSKDSPKIGSPSAESVKVEKFQ 120

Query: 121 KSSSMKVERVSKVEQSDRNGTTSSVKYSRSESAISEKNIVKVEKTDSLEKVKAERVVKEE 180
           KSSSMKVERVSKVEQ DRNG TSSVKYS+SES +SE+N VKVEKTDS+  VK ERVVKEE
Sbjct: 121 KSSSMKVERVSKVEQFDRNGATSSVKYSKSESVVSERNSVKVEKTDSM--VKVERVVKEE 180

Query: 181 KKISIDKRPSSGTAAPPKLTSMIKSKDAARDKIRELLCEAFSKVPGEADEDVIDEVNASD 240
           KK      PSSG AAPPKLTSMIKSKDAARDKIRELL EAFSKVPGEADE+ +DEVNASD
Sbjct: 181 KK------PSSGAAAPPKLTSMIKSKDAARDKIRELLFEAFSKVPGEADEEFMDEVNASD 240

Query: 241 PIRVAVSVESVMFENWGGSTGAQKAKYRSIMFNLKDPKNPDFRRNVLVGHIKPERLINMS 300
           PIRVAVSVESVMFENWGGSTGAQKAKYRSIMFNLKDPKNPDFRR VL+G IKPER+INMS
Sbjct: 241 PIRVAVSVESVMFENWGGSTGAQKAKYRSIMFNLKDPKNPDFRRKVLLGLIKPERMINMS 300

Query: 301 TAEMASDQRKRENEEIEQKALFDCERGGAPKATTDQFKCGRCGQRKTTYYQLQTRSADEP 360
           TA+MASDQRKRENEEI QKALFDCERGGAPKATTDQFKCGRCGQRKTTYYQLQTRSADEP
Sbjct: 301 TADMASDQRKRENEEIAQKALFDCERGGAPKATTDQFKCGRCGQRKTTYYQLQTRSADEP 360

Query: 361 MTTFVTCVNCNNHWKFC 378
           MTTFVTCVNCNNHWKFC
Sbjct: 361 MTTFVTCVNCNNHWKFC 369

[Close]

BLAST of MS003348 vs. ExPASy TrEMBL
Match: A0A6J1JLK7 (transcription elongation factor TFIIS-like OS=Cucurbita maxima OX=3661 GN=LOC111486982 PE=4 SV=1)

HSP 1 Score: 647.1 bits (1668), Expect = 4.5e-182
Identity = 341/377 (90.45%), Postives = 356/377 (94.43%), Query Frame = 0


Query: 1   MENELVELFEAAKKAADAAAAPSNDGGAEESRCLDALRQLKKFPVTYQILVSTQVGKRLR 60
           ME ELVELFEAAKKAADAAAAPSNDGGAEESRCLDALRQLKKFPVTYQILVSTQVGKRLR
Sbjct: 1   MEKELVELFEAAKKAADAAAAPSNDGGAEESRCLDALRQLKKFPVTYQILVSTQVGKRLR 60

Query: 61  HLTKHPKKKIQDYASDLIEMWKEIVIKETNKNKKNGNGNCKESPKIGSPSADSVKVEKFQ 120
           HLTKHPKKKIQDYASDLIEMWK+IVIKETNKNKKNGNGN KE  KIGSPSA+SVKVE FQ
Sbjct: 61  HLTKHPKKKIQDYASDLIEMWKDIVIKETNKNKKNGNGNSKEPSKIGSPSAESVKVENFQ 120

Query: 121 KSSSMKVERVSKVEQSDRNGTTSSVKYSRSESAISEKNIVKVEKTDSLEKVKAERVVKEE 180
           KSSSMKVERVSKVEQ +R G+TSSVK+SRS SA+SEKN VKVEKTDS+  VK ER+VKEE
Sbjct: 121 KSSSMKVERVSKVEQFNRIGSTSSVKFSRSASAVSEKNSVKVEKTDSV--VKVERMVKEE 180

Query: 181 KKISIDKRPSSGTAAPPKLTSMIKSKDAARDKIRELLCEAFSKVPGEADEDVIDEVNASD 240
           KKISI+K+P SG A PPKLTSMIKSKDAARDKIRELL EAFSKVPGEADEDVIDEVNASD
Sbjct: 181 KKISIEKKP-SGAAGPPKLTSMIKSKDAARDKIRELLVEAFSKVPGEADEDVIDEVNASD 240

Query: 241 PIRVAVSVESVMFENWGGSTGAQKAKYRSIMFNLKDPKNPDFRRNVLVGHIKPERLINMS 300
           PIRVAVSVESVMFENWGGSTGAQKAKYRSIMFNLKDPKNPDFRR VL+G IKPER+ N+S
Sbjct: 241 PIRVAVSVESVMFENWGGSTGAQKAKYRSIMFNLKDPKNPDFRRKVLLGLIKPERMTNLS 300

Query: 301 TAEMASDQRKRENEEIEQKALFDCERGGAPKATTDQFKCGRCGQRKTTYYQLQTRSADEP 360
           T +MASDQRKRENEEI QKALF+CERGGAPKATTDQFKCGRCGQRKTTYYQLQTRSADEP
Sbjct: 301 TTDMASDQRKRENEEIAQKALFECERGGAPKATTDQFKCGRCGQRKTTYYQLQTRSADEP 360

Query: 361 MTTFVTCVNCNNHWKFC 378
           MTTFVTCVNCNNHWKFC
Sbjct: 361 MTTFVTCVNCNNHWKFC 374

[Close]

BLAST of MS003348 vs. ExPASy TrEMBL
Match: A0A6J1GYA3 (transcription elongation factor TFIIS-like OS=Cucurbita moschata OX=3662 GN=LOC111458597 PE=4 SV=1)

HSP 1 Score: 646.7 bits (1667), Expect = 5.8e-182
Identity = 341/377 (90.45%), Postives = 355/377 (94.16%), Query Frame = 0


Query: 1   MENELVELFEAAKKAADAAAAPSNDGGAEESRCLDALRQLKKFPVTYQILVSTQVGKRLR 60
           ME ELVELFEAAKKAADAAAA SNDGGAEESRCLDALRQLKKFPVTYQILVSTQVGKRLR
Sbjct: 1   MEKELVELFEAAKKAADAAAARSNDGGAEESRCLDALRQLKKFPVTYQILVSTQVGKRLR 60

Query: 61  HLTKHPKKKIQDYASDLIEMWKEIVIKETNKNKKNGNGNCKESPKIGSPSADSVKVEKFQ 120
           HLTKHPKKKIQDYASDLIEMWK+IVIKETNKNKKNGNGN KE  KIGSPSA+SVKVE  Q
Sbjct: 61  HLTKHPKKKIQDYASDLIEMWKDIVIKETNKNKKNGNGNSKEPSKIGSPSAESVKVENLQ 120

Query: 121 KSSSMKVERVSKVEQSDRNGTTSSVKYSRSESAISEKNIVKVEKTDSLEKVKAERVVKEE 180
           KSSSMKVERVSKVEQ DRNG+TSSVK+SRS SA+SEKN VKVEKTDS+  VK ER+VKEE
Sbjct: 121 KSSSMKVERVSKVEQFDRNGSTSSVKFSRSASAVSEKNSVKVEKTDSV--VKVERMVKEE 180

Query: 181 KKISIDKRPSSGTAAPPKLTSMIKSKDAARDKIRELLCEAFSKVPGEADEDVIDEVNASD 240
           KKISI+K+P SG A PPKLTSMIKSKDAARDKIRELL EAFSKVPGEADEDVIDEVNASD
Sbjct: 181 KKISIEKKP-SGAAGPPKLTSMIKSKDAARDKIRELLVEAFSKVPGEADEDVIDEVNASD 240

Query: 241 PIRVAVSVESVMFENWGGSTGAQKAKYRSIMFNLKDPKNPDFRRNVLVGHIKPERLINMS 300
           PIRVAVSVESVMFENWGGSTGAQKAKYRSIMFNLKDPKNPDFRR VL+G IKPER+ N+S
Sbjct: 241 PIRVAVSVESVMFENWGGSTGAQKAKYRSIMFNLKDPKNPDFRRKVLLGLIKPERMTNLS 300

Query: 301 TAEMASDQRKRENEEIEQKALFDCERGGAPKATTDQFKCGRCGQRKTTYYQLQTRSADEP 360
           T +MASDQRKRENEEI QKALF+CERGGAPKATTDQFKCGRCGQRKTTYYQLQTRSADEP
Sbjct: 301 TTDMASDQRKRENEEIAQKALFECERGGAPKATTDQFKCGRCGQRKTTYYQLQTRSADEP 360

Query: 361 MTTFVTCVNCNNHWKFC 378
           MTTFVTCVNCNNHWKFC
Sbjct: 361 MTTFVTCVNCNNHWKFC 374

[Close]

BLAST of MS003348 vs. TAIR 10
Match: AT2G38560.1 (transcript elongation factor IIS )

HSP 1 Score: 387.9 bits (995), Expect = 9.4e-108
Identity = 221/384 (57.55%), Postives = 273/384 (71.09%), Query Frame = 0


Query: 1   MENELVELFEAAKKAADAAAAPS-NDGGAEESRCLDALRQLKKFPVTYQILVSTQVGKRL 60
           ME++L++LFE AKKAADAAA       G E S+C+DAL+QLKKFPVTY  LV+TQVGK+L
Sbjct: 1   MESDLIDLFEGAKKAADAAALDGVTSSGPEVSQCIDALKQLKKFPVTYDTLVATQVGKKL 60

Query: 61  RHLTKHPKKKIQDYASDLIEMWKEIVIKETNKNKK-NGNGNCKESP----KIGSPSADS- 120
           R L KHP + I+  A+DL+E+WK++VI+ET K KK  G   CKE+      +  PS  + 
Sbjct: 61  RSLAKHPVEDIKSVATDLLEIWKKVVIEETAKAKKTEGTNGCKEAKVNKMDVEKPSNPAP 120

Query: 121 VKVEKFQKSSSMKVERVSKVEQSDRNGTTSSVKYSRSESAISEKNIVKVEKTDSLEKVKA 180
           VKV+K Q+  S K  +V + E    N   + VK  R    I   N  K++     + VK 
Sbjct: 121 VKVQKLQRGDSAKSIKVERKEPD--NKVVTGVKIERKVPDIKVTNGTKIDYRG--QAVKD 180

Query: 181 ERVVKEEKKISIDKRPSSGTAAPPKLTSMIKSKDAARDKIRELLCEAFSKVPGEADEDVI 240
           E+V K+ +  S  K P+    APPKLT+M+K  D  RDKIRELL EA  +V GEAD+   
Sbjct: 181 EKVSKDNQ--SSMKAPAKAANAPPKLTAMLKCNDPVRDKIRELLVEALCRVAGEADDYER 240

Query: 241 DEVNASDPIRVAVSVESVMFENWGGSTGAQKAKYRSIMFNLKDPKNPDFRRNVLVGHIKP 300
           + VNASDP+RVAVSVES+MFE  G STGAQK KYRSIMFNL+D  NPD RR VL G I P
Sbjct: 241 ESVNASDPLRVAVSVESLMFEKLGRSTGAQKLKYRSIMFNLRDSNNPDLRRRVLTGEISP 300

Query: 301 ERLINMSTAEMASDQRKRENEEIEQKALFDCERGGAPKATTDQFKCGRCGQRKTTYYQLQ 360
           E+LI +S  +MASD+RK+EN +I++KALFDCERG A KA+TDQFKCGRCGQRK TYYQ+Q
Sbjct: 301 EKLITLSAEDMASDKRKQENNQIKEKALFDCERGLAAKASTDQFKCGRCGQRKCTYYQMQ 360

Query: 361 TRSADEPMTTFVTCVNCNNHWKFC 378
           TRSADEPMTT+VTCVNC+NHWKFC
Sbjct: 361 TRSADEPMTTYVTCVNCDNHWKFC 378

[Close]

BLAST of MS003348 vs. TAIR 10
Match: AT2G42730.1 (F-box family protein )

HSP 1 Score: 105.5 bits (262), Expect = 9.3e-23
Identity = 60/127 (47.24%), Postives = 80/127 (62.99%), Query Frame = 0


Query: 197 PKLTSMIKSKDAARDKIRELLCEAFSKVPGE-ADEDVIDEVNASDPIRVAVSVESVMFEN 256
           P  ++M K+ D+ RDK+RE+L  +  KV  E  D ++   V A DP  VAVSVES MFE 
Sbjct: 594 PTHSTMKKTGDSKRDKVREILQTSLVKVASEIVDTEMKTRVTACDPSVVAVSVESAMFEK 653

Query: 257 WGGSTGAQKAKYRSIMFNLKDPKNPDFRRNVLVGHIKPERLINMSTAEMASDQRKRENEE 316
            G   G  KAKYRSI+FN+ D  NPD RR VL+G I  ERL+ M   EM S++ ++E + 
Sbjct: 654 LGCFMGPHKAKYRSILFNMGDSNNPDLRRKVLIGEINGERLVTMERQEMGSEKIQKEVQR 713

Query: 317 IEQKALF 323
           I++ A F
Sbjct: 714 IKENARF 720

[Close]

BLAST of MS003348 vs. TAIR 10
Match: AT4G18720.1 (Transcription factor IIS protein )

HSP 1 Score: 101.3 bits (251), Expect = 1.8e-21
Identity = 57/111 (51.35%), Postives = 71/111 (63.96%), Query Frame = 0


Query: 197 PKLTSMIKSKDAARDKIRELLCEAFSKVPGE-ADEDVIDEVNASDPIRVAVSVESVMFEN 256
           P   +M K+ D+ RDK+RE+L  + +KV  E  D ++   V A DP  VAVSVE+ MFEN
Sbjct: 105 PTHATMKKTGDSKRDKVREILQTSLAKVASEVVDTEMKTRVTACDPWVVAVSVETAMFEN 164

Query: 257 WGGSTGAQKAKYRSIMFNLKDPKNPDFRRNVLVGHIKPERLINMSTAEMAS 307
            G   G QKAKYRSI+FN+ D  NPD RR VL+G I  ERL+ M   EM S
Sbjct: 165 LGCFMGPQKAKYRSILFNMGDSNNPDLRRKVLLGEISGERLVKMEKEEMGS 215

[Close]

BLAST of MS003348 vs. TAIR 10
Match: AT5G42325.1 (Transcription factor IIS protein )

HSP 1 Score: 72.0 bits (175), Expect = 1.1e-12
Identity = 47/128 (36.72%), Postives = 70/128 (54.69%), Query Frame = 0


Query: 197 PKLTSMIKSKDAARDKIRELLCEAFSKVPGEADEDVIDE--VNASDPIRVAVSVESVMFE 256
           P  ++M K+ D+ RDK+ E+L  + +KV  E  +  +    +   DP  VAVSVES M  
Sbjct: 104 PTHSTMKKTGDSKRDKVHEILQSSLAKVATEVVDTEMKRRVMTVCDPWVVAVSVESAM-- 163

Query: 257 NWGGSTGAQKAKYRSIMFNLKDPKNPDFRRNVLVGHIKPERLINMSTAEMASDQRKRENE 316
                         SI+FN+ D  NPD RR VL+G I  ERL+ M   EM S++ ++E +
Sbjct: 164 --------------SILFNMGDSNNPDLRRKVLIGEISGERLVKMEKDEMGSEKIQKEVQ 215

Query: 317 EIEQKALF 323
            I+++A F
Sbjct: 224 RIKERARF 215

[Close]

BLAST of MS003348 vs. TAIR 10
Match: AT5G25520.1 (SPOC domain / Transcription elongation factor S-II protein )

HSP 1 Score: 62.8 bits (151), Expect = 6.9e-10
Identity = 34/83 (40.96%), Postives = 51/83 (61.45%), Query Frame = 0


Query: 240 DPIRVAVSVESVMFENWGGSTGAQKAKYRSIMFNLKDPKNPDFRRNVLVGHIKPERLINM 299
           DP  +A  +E  +F+ +GG     K K RS++FNLKD  NP+ R +V+ G I PERL NM
Sbjct: 359 DPELLASKIELELFKLFGGVNKKYKEKGRSLLFNLKDKNNPELRESVMSGKISPERLCNM 418

Query: 300 STAEMASDQ----RKRENEEIEQ 319
           +  E+AS +    R+ + EE+ +
Sbjct: 419 TAEELASKELSQWRQAKAEEMAE 441

The following BLAST results are available for this feature:

BLAST of MS003348 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022134049.1	3.9e-204	99.20	transcription elongation factor TFIIS [Momordica charantia] >XP_022134050.1 tran...	[more]
XP_038884917.1	8.6e-188	91.78	transcription elongation factor TFIIS [Benincasa hispida]	[more]
KAG6578486.1	3.4e-184	90.98	Transcription elongation factor TFIIS, partial [Cucurbita argyrosperma subsp. so...	[more]
KAG7016050.1	1.7e-183	90.72	Transcription elongation factor TFIIS, partial [Cucurbita argyrosperma subsp. ar...	[more]
XP_022938772.1	2.9e-183	90.45	transcription elongation factor TFIIS-like [Cucurbita moschata]	[more]

BLAST of MS003348 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9ZVH8	1.3e-106	57.55	Transcription elongation factor TFIIS OS=Arabidopsis thaliana OX=3702 GN=TFIIS P...	[more]
P20232	5.9e-30	29.44	Transcription elongation factor S-II OS=Drosophila melanogaster OX=7227 GN=TfIIS...	[more]
Q2KI09	1.3e-29	31.10	Transcription elongation factor A protein 3 OS=Bos taurus OX=9913 GN=TCEA3 PE=2 ...	[more]
P23881	6.5e-29	30.61	Transcription elongation factor A protein 3 OS=Mus musculus OX=10090 GN=Tcea3 PE...	[more]
P10711	3.2e-28	28.76	Transcription elongation factor A protein 1 OS=Mus musculus OX=10090 GN=Tcea1 PE...	[more]

BLAST of MS003348 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1BWY6	1.9e-204	99.20	transcription elongation factor TFIIS OS=Momordica charantia OX=3673 GN=LOC11100...	[more]
A0A6J1FF11	1.4e-183	90.45	transcription elongation factor TFIIS-like OS=Cucurbita moschata OX=3662 GN=LOC1...	[more]
A0A0A0KDH9	6.9e-183	90.72	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G014610 PE=4 SV=1	[more]
A0A6J1JLK7	4.5e-182	90.45	transcription elongation factor TFIIS-like OS=Cucurbita maxima OX=3661 GN=LOC111...	[more]
A0A6J1GYA3	5.8e-182	90.45	transcription elongation factor TFIIS-like OS=Cucurbita moschata OX=3662 GN=LOC1...	[more]

BLAST of MS003348 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G38560.1	9.4e-108	57.55	transcript elongation factor IIS	[more]
AT2G42730.1	9.3e-23	47.24	F-box family protein	[more]
AT4G18720.1	1.8e-21	51.35	Transcription factor IIS protein	[more]
AT5G42325.1	1.1e-12	36.72	Transcription factor IIS protein	[more]
AT5G25520.1	6.9e-10	40.96	SPOC domain / Transcription elongation factor S-II protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25

Position :

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x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 299..319
None	No IPR available	GENE3D	2.20.25.10		coord: 306..377 e-value: 1.1E-27 score: 97.6
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 136..150
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 119..135
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 91..150
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 91..106
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 185..204
None	No IPR available	PANTHER	PTHR11477	TRANSCRIPTION FACTOR S-II ZINC FINGER DOMAIN-CONTAINING PROTEIN	coord: 1..377
None	No IPR available	PANTHER	PTHR11477:SF36	TRANSCRIPTION ELONGATION FACTOR TFIIS	coord: 1..377
None	No IPR available	CDD	cd13749	Zn-ribbon_TFIIS	coord: 330..376 e-value: 1.2261E-29 score: 106.536
None	No IPR available	CDD	cd00183	TFIIS_I	coord: 24..85 e-value: 1.44687E-16 score: 71.9558
None	No IPR available	SUPERFAMILY	57783	Zinc beta-ribbon	coord: 316..375
IPR001222	Zinc finger, TFIIS-type	SMART	SM00440	Cys4_2	coord: 337..376 e-value: 9.7E-24 score: 94.9
IPR001222	Zinc finger, TFIIS-type	PFAM	PF01096	TFIIS_C	coord: 337..375 e-value: 1.1E-19 score: 70.0
IPR001222	Zinc finger, TFIIS-type	PROSITE	PS00466	ZF_TFIIS_1	coord: 339..374
IPR001222	Zinc finger, TFIIS-type	PROSITE	PS51133	ZF_TFIIS_2	coord: 335..375 score: 14.636859
IPR003617	Transcription elongation factor, TFIIS/CRSP70, N-terminal, sub-type	SMART	SM00509	TFS2_5	coord: 8..87 e-value: 5.3E-24 score: 95.7
IPR003618	Transcription elongation factor S-II, central domain	SMART	SM00510	mid_6	coord: 211..315 e-value: 3.5E-37 score: 139.5
IPR003618	Transcription elongation factor S-II, central domain	PFAM	PF07500	TFIIS_M	coord: 207..323 e-value: 3.8E-33 score: 114.4
IPR003618	Transcription elongation factor S-II, central domain	PROSITE	PS51321	TFIIS_CENTRAL	coord: 209..332 score: 33.545177
IPR035441	TFIIS/LEDGF domain superfamily	GENE3D	1.20.930.10	Conserved domain common to transcription factors TFIIS, elongin A, CRSP70	coord: 1..100 e-value: 9.6E-24 score: 85.0
IPR035441	TFIIS/LEDGF domain superfamily	SUPERFAMILY	47676	Conserved domain common to transcription factors TFIIS, elongin A, CRSP70	coord: 27..98
IPR036575	Transcription elongation factor S-II, central domain superfamily	GENE3D	1.10.472.30		coord: 187..305 e-value: 8.5E-28 score: 98.8
IPR036575	Transcription elongation factor S-II, central domain superfamily	SUPERFAMILY	46942	Elongation factor TFIIS domain 2	coord: 196..307
IPR006289	Transcription elongation factor, TFIIS	TIGRFAM	TIGR01385	TIGR01385	coord: 158..377 e-value: 4.7E-81 score: 270.7
IPR035100	Transcription elongation factor, IIS-type	PIRSF	PIRSF006704	TFIIS	coord: 2..377 e-value: 1.3E-97 score: 325.0
IPR017923	Transcription factor IIS, N-terminal	PFAM	PF08711	Med26	coord: 36..85 e-value: 3.1E-11 score: 43.1
IPR017923	Transcription factor IIS, N-terminal	PROSITE	PS51319	TFIIS_N	coord: 10..88 score: 20.508789

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS003348.1	MS003348.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006355	regulation of transcription, DNA-templated
biological_process	GO:0006351	transcription, DNA-templated
cellular_component	GO:0005634	nucleus
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0008270	zinc ion binding