MS003287 (gene) Bitter gourd (TR) v1

Overview
NameMS003287
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptiontRNA (guanine(26)-N(2))-dimethyltransferase
Locationscaffold234: 1792138 .. 1799295 (-)
RNA-Seq ExpressionMS003287
SyntenyMS003287
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGACAGATCTTAGTGAATTCACCATCATCAAGGAGGGAGAGGCTGAGATTCTCATGCACGCGAAGAATGAAGTCTTTTTCAATAAGACCCAGGTGCCTTGTCTATTTTTCAATGATGGTTTCTGGAGTTTTGGAGTTAAGGCATTGTCCTTAACTAAACTTAATGGGTCATTCAAAAAATTTCCTCGTTTTTTCTTTTACTTTCTTCGCCCATGGATGCTAAAGGTGGGGGAAGAGCTTACACATGATTGGTACTAGCATTTGAAGTTAGATTAGGGGAAATTTCTATTGGTGAAGTGAAGGGGCTTTGTGATTGATGCCACTATATTGGAACTTCAGAATTTTAGCATATTTAAAGATAGATATTTGATGGAGAAACTAAGATATACAATTTATTCAAGTCAGCAAAGTAAAATGTGGTATAGTAAATTGTAAGATATTGTTCCATTCAGTATGCACAACATGACGGCAGTTTTCTTAATGTTATGAATGATTAATCATCGGCCTATAGCTGCATTGCTTTATTACATAATTTGTTTTAGGTCCCAGAGTCTTCCCTTCAGTAATTAAGAGTATCTGGGAATCTTTCTTTTACTTCATGGAAATTGTTACCTACTTTATTCTATCTGTAATATCATCATATAATGAAGGGTTGTGGGAGTTGTACTTACCCTTTTTTGGCTAGCTTCAAACATAATATGTCTAGCTTCAAACTTTTTCTCATTGTAGAATCTTGACTGTTCACCATTGTAATATTCAGGTTAACAACAGAGACATATCCATTGCTGTCCTTAGGGCATTTATATCAAAACGCAAACAGGAGCACGAAGCAAAACTATCAAGAAGAACAAAAGCCGGGCAAAAGATTTCTGAAAATAATGTTTCTGGACCTGTTGTGGAAGAAACACCTAATGAATCTGCCACAAATGATGAAAAGTCAAATACAGATCGTGATGCTCATGAAGAAACACCGGATGAATGTGGAGTTCCAAAGTTGTCAGAAGAACAAAAAGAGATCATCGAGGAAAAAGAACAAAGAGATCCCAAGCCTCTTAGAGTTCTCGAGGTTCCTTTTATTTCAATGATCATCTTATTACGTGCATAATTTAATTGATGTTTATTATTCTATGAAGTGTGGACATTTTAATTTAGGAATATGTCCATGTCTGGATTATCAGTACCCTGTTTCAGTAATACAGAGTCTGTAATCTGTCTTTCCCCTAATAAGCTACCTAAATTCTGAACTGATGCTACTACTAGAAAGGACCTTCTTGAGATGCACTACAAGAAGTTTGGTGACTATTTATTAATTAGCAGTTGTTAGGGTAAGAGGCCTGAGTCTGTTCTGAGAATTTTTTGGAATAAGATGACCGAGAGTTTCATTAGTGCTGAAATCGAAGACTTCCTTTACCACAGAAAGTTCTGGAACAATGCAACACAGCTGGGGCTTTTCCAAGTCTAAGGTCTTAGGTGGTTGCAATATTTACCCTCCCCCAAACGTTTCCTCCAATTGTTAGTGACCTTGAGTTGAATTGGCCACTAGAGGACATAATTCTTTTTAAATAAATGGAAGACCAAGTGGAAGGGTAAGGATATAACAGGAAAAAAAAAAATTGTTTAAAATGATGAATTTTGAATGAAGATACTTCTACGTATCTGATATTTGAATTTTTGCTTTTGATATGATAGATAGGTAATTAGTTAAGGTTTTAAAAAAATCTGATTTTAGTTTTCCTGTATATTATTTTGCTATACATCATTGCATTATTAAACATTTTGTAACTGGCTTCTCAAATTCTTACAGATGTTGTATAGCTTATCCTCTCATTCATATAGTTGGAATTCAAGTAACATAATGTAAAGTGCATATTTTATGTGAAAGTTGAAAATTATTTAGAAGCGTCAAAATATACAAATATATTAGATACAAGTTGTTATGAATAGTGTGTTTTTGTGCCATGCTCATGTCCATGAATATGAATGTCACCCCATTCTAACATGTTAGAATTGAATGTTCACCATGTTGTCATTGTGTTTTTTTTACTCCGATGTTATATTTGTGCTTCTAAAACTTTTTATGTGTGTTCCCTTCTTAAGGGCCTTTTGTAATTTCATACATCAATGAAATTGTTTCTTTCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACTTTTTATGTGTGTTTGCTTCACTTTTGGGATTCCTGATGTGGGTATAGTGATTTGTTGTCTATTCACTATTTTCTGAGTACTTTCTGTTTAACTTTGAAGGCCTTGTCGGCTTCAGGGCTAAGAGCCTTAAGATATGCTCGTGAAGTAGAGGGCGTTGGTCAAGTTGTGGCGCTAGACAATGATAAAGGTAATTTCTTGGTTACTGTAATATCAAAAAGTTACTTTAGAATGTTTCTTGTCAAAAATAAAACAAAGGTTAAAAAATCTTGTAAGAAATCTAAAGTTTACGAGATTGTTAGGTTATCTGTTATTCATATCAATGGATACCTTTATGCACACACTTCATACATGCACGAAGAGCTCACAGTGGTTTCTGATCATAAAGCTCTCTACTTCCACAAGGTGTTTAATAAGTGGATTTTGGAATATTGCCAATGCTTGACAGTTGGCACTACCCCAAATCTGATACGGAAGGTACCTTTGCCTTGTACAGGCAGAGGAAGTTAGTGTTGATAATCTTTTCCCTTGTTATCTATATTCAAATGTTTTTTTTAACTCGATGCAAACTTCTCATTGAAATAATGAAGATAAAATACAAAATTTAGGAGTGTATTCTTTGTATTTATCTCTGTAAATCATTAATTGATGTCTGTCAGTGACACCATAAAAATGGAAGTTATCCATTTTTACTGTTCTTACAATATGTTCTTATCTATATTGATATGACATCTAGGTGTTGGTTACTAACTGAACCTGTTAATAATAAGAATTGTCTTTCTACAGCATCAGTGGAAGCTTGTCGAAGAAACATCAAGTTTAATGGTTCAGTAGCATGTGCGAAGGTGGAATCACATCTTACTGATGCTCGTGTTTATATGCTCACTCACCCAAAAGAATTTGATATGGTATGCATGCACTATTTAAGATATGTAGGTTATCTCTTTAGAAATTACATATAGGGAAGTAATTATTGGAATGCATTTTCTTTTTCCTTGTTATAGTTTAGATAATAACCATGTATAACTTAATTTCCTTGTTATAGTTTGCTGTTCTTTAGGGTTTGTGGTATGAGAGGAAACAAGGGGTAGAGAGTTCTAGGGAGGAGGTGTCAGCTCTTGTTCAAAGTATTTTGTGATTATTCTTAGGTTTGATCTTGCTTGATTGGAGCTCGTTCTTTTAATTTGGCTCCCTTGGTTTGGCTCTTTTTTTGTATGCTCCTTTGTATTCTTTCATTTTTCAAAATGAAAGCTCGGCTTTTCATTAAAAAAAAAAAAAACCAGTATACCTTAATGATTTAAGCTCTGACATGAATGCAAGTATCAAGCTTTATCAACTTATTTGGATATTTTCAGGTTGATCTGGATCCTTATGGTTCACCTTCTGTTTTCTTGGACTCTGCTGTTCAATCAGTTGTCGATGGTGGTATGCTGATGTGCACAGCAACTGATATGGCGGTGCTTTGTGGGGGAAATGGAGAAGTTTGCTATTCAAAGTAAGGTTTTTTTGGGTCCTCAAATATTATTATTGTTTCAATTTCTGCTGTAACTGATTCTGTGTCTATAAATTGCTGACTGATTCAGATATGGTTCCTATCCTTTGAGAGGGAAATATTGCCACGAAATGGCTTTAAGAATCCTCCTTGCCTGCATTGAGGTATGTCTATCTCCTGTGTATTTAAAAAGTTTTAATAATTTATTTTTTCACCTTTTCTTTTCTCTTTCTTTTTCCTTCTCTGGTTTGACTTGCCGTATGAGATCATTCTTATCTTAGTAAAAACATGCGGTATATAAAGAGTGAAGAGGAAACTGAGATCATTCATTTATATGTATTTATGTACATATTTTATAAGAAACTGAGAATCTATTGAGGGGAGTACAAAAAAGGATCCCAATTAGCACAAATAAGCAAAAGAAGTGTAATTATAAAAAGAATAGGATGAGGTACAGCACCAAGAAGCATAGTGTCTAAGTAGGTCCAAACATTCTTCCAAAGGAGAGAAAGAGTCTTTGAAAATCCTTTGGTTCCTGTCATACCCAAGCCTCCATAAAGAGTGAAGAGGGTATTAGTCCAAAGGATTTTGGCCTTCCCGGAGAAAGGGTGGCCGCACTGAATCTGAGGTATCAATGATCTGCTACATGAAGGGAAATACCAAGCAACTCCTGTTTATGAGCTTTTATCACTCAAGAAATATACATGTATGAACGCGTATATACATCTTCAGAAGTGTTGATATCCAAGTTGACACTCATTATATTGTTAACTAAAGTATATAGCTCTGCCTGTTTATGAGTTTTTTGTCATTCAAGGAATAAGTTATTTTCTTTATTTTTAGTTAATTTGAGTCGTATGGTGCTTCACCTGCCTCTTTATTCTAATCTTTAGAACATGCGAGCTTCTTTAACCGAAATATAACATACTTCAAATGCTTGGTGTGGTCTCTTATGCATTATTTTTGTTGTTCTAAATTTTGTAGAGCCATGCAAATAGATACAAGCGGTACATTGTTCCTGTGCTATCTGTTCAGATGGATTTCTACGTTCGTGTTTTTGTTCGTATTTACACGTAAGTGCCTAAGTCTTCAAATTTAGTCAGCTAGACGGTATTCCACTACTTCTTTTACGTGGTATAATTATGATATTAATATAAAAATGATGTAGTTTCTGTTGATGTTAACACTTAGCAAAATAATTGGCTGCAGTTCTGCAAGTGCAATGAAAAATACTCCCCTTAAGCTCTCATATGTTTATCAGTGCACTGGTTGTGATTCTTTCCATCTCCAGCAGATTGGGAGAAGCGTCTCGAAGGTATATACCACTAAGACCTGGTATTTATGCTAGTTATTACATAATTGAGTTTATTTTTATTATCCAGTGTAACTTCTCAAACTGGTTCCTGTGACAAGTTTCTCATGCTTATTTTTACACTTACCTCTTATTTATTTATGATTATTTTTGTGGAAAAACTAGCTTTCATTCAATTGAATGAAATAAAACTAACAGCCAACAAAAACACAATGGCCAGCCAAAACTGGAGAACACAAGAAAAAAATTACGAGAATGGGTCCTAACTTTGGTGATAAAAGTTTATTACAAAAAAGGTTCGAAATATGAGTGCAAAGGAAGGCATTAAAATGCACCAGCCCTTCACCTCTTCCCACCACCTGTCTAATCCCATAAACGTTCTCCCATTTCTAAAGCTTGTTTTCCAAAGTACACTACTACCTTTCTTTATAAACAGAGGATCCAAAACAATGTCCTCCATCAACCCTCAAATGTCGGCTTTTTAACCAGATAAATATCAAAAGCCTGCTACAAATAATCCACACTTCAGAAGCAAGAGAGTATGGCCAAAGGAGGGGTGATTAAGATCCTCTGCATCACACTTGCACCAAGCGCACCAATTTGGCGAACAAAAATAGAGCCTCTCGGCAGGAAGTTAACATCAACAAGTCAAGAAAAGAACTGAAATTTCATTAAAACTTGATATTCAGAATCTGGCTGATGTAAGCTATCGATTTTGTATCAATATTTAATTTTGGTTGGAAGTTATGATAGTTGAAGTAGTAATTTCTTTATAATATGAACTTCTATAGTTTCTTGACGTGTCACTATCCTTCTGCTTTCAGAACAACAGCATCAGGTATTTACCTGGTTTTGGTCCTTCTGTTCCTCAAGAGTGCAGTGATTGTGGGAAGAAATTCAACATGGGTGGGCCTATCTGGTCTGCTCCTATCCATGATCAAGAATGGGTTACTTCCATGCTAGCAGACGTGAAGTCTATGAAGGACTCATATCCTGCATATGATCGTATATCTGCTGTATTGACAACAATTTCAGAGGTGTGCCTTATGTGCGGACAATAGTGATTTTAGAGTAAACACTTTATTAGTAACACCATGGTTAAAAGGCTCTCAAACATACTAAAAATCATCTTGCTATTATGTGCTGACGAATGGTTCTAACTTGTGAAGGATTAAGTACAATAGTGATTTTAGAGTAAACACTTTATTAGTAACACCATGGTTAAAAGGCTCTCAAACATACTCTAAATCATCTTGCTATTATGTGCTGATGAATGGTATTCATGTACAGCAATTATAAACTCGGATGTATTTGTTACTATGCAGGAATTGATGGATGTTCCCTTATTTTTGAGTCTACACAACCTCTGTGCAACATTGAAGTGCACATCTCCATCAGCTGTAAACTTCCGTTCTGCAGTGATTAATGCAGGATATCGCATATCGGGCACTCATGTGAATCCTTTAGGACTGAAATCAGATGCTCCCATGGATGTAATTTGGGATATTATGCGTTGCTGGGTAAGGCACACCCAATGCTACATTTCATTATATACTTGAATGTCACTTTATAATATCATAGCCTTGTGATCAGGAGTGGAACTGTAGACCTTCCTTAAATGTACTTTCATCCTAATTTCATCTACTGAAGATGGGGCCTTCTTGATTACCTGAAGAGATTTTTGAGTTTTCAGTGATCCTTTTTCATGTAGGTCAAAAACCACCCGGTGAAACCTCAACCAGAAGATCAGCCAGGAAGTGTCATTCTTGCTAAGGAACCGGTGCTTCAAGTAAGTTCCATCTTGTAGTTCGAGTCTTGTCGAACATGTTATTGTCCGGGGACTAAACTTTGGGTTACCACCATTCACATTCAAACACATAAAACAAAAGGCCTGTGGCTGATATACTTTTGCCAATTCTTATCTTCTTGACGCAGGCTAATTTTGCTAGAGCTGTGGCATCTCTTAGCAAGGCTCAAGCAAAGAAAGTTGCACGTTTCCTTCCAAATCCTGAAAGACATTGGGGCCCCAAGCTGAGAGCAGGTCGTCAAATCACTAGTAAGCACATCTCTCTACTGGGCGCAGAGGCAGTAAACGGAGCTCTTAGCCATACAGATTCTGAAGAGCCTAATGCAAAACGCCCCAATGTAGAAGAAGTTGCTCTTAGCCATACG

mRNA sequence

ATGGCGACAGATCTTAGTGAATTCACCATCATCAAGGAGGGAGAGGCTGAGATTCTCATGCACGCGAAGAATGAAGTCTTTTTCAATAAGACCCAGGTTAACAACAGAGACATATCCATTGCTGTCCTTAGGGCATTTATATCAAAACGCAAACAGGAGCACGAAGCAAAACTATCAAGAAGAACAAAAGCCGGGCAAAAGATTTCTGAAAATAATGTTTCTGGACCTGTTGTGGAAGAAACACCTAATGAATCTGCCACAAATGATGAAAAGTCAAATACAGATCGTGATGCTCATGAAGAAACACCGGATGAATGTGGAGTTCCAAAGTTGTCAGAAGAACAAAAAGAGATCATCGAGGAAAAAGAACAAAGAGATCCCAAGCCTCTTAGAGTTCTCGAGGCCTTGTCGGCTTCAGGGCTAAGAGCCTTAAGATATGCTCGTGAAGTAGAGGGCGTTGGTCAAGTTGTGGCGCTAGACAATGATAAAGCATCAGTGGAAGCTTGTCGAAGAAACATCAAGTTTAATGGTTCAGTAGCATGTGCGAAGGTGGAATCACATCTTACTGATGCTCGTGTTTATATGCTCACTCACCCAAAAGAATTTGATATGGTTGATCTGGATCCTTATGGTTCACCTTCTGTTTTCTTGGACTCTGCTGTTCAATCAGTTGTCGATGGTGGTATGCTGATGTGCACAGCAACTGATATGGCGGTGCTTTGTGGGGGAAATGGAGAAGTTTGCTATTCAAAATATGGTTCCTATCCTTTGAGAGGGAAATATTGCCACGAAATGGCTTTAAGAATCCTCCTTGCCTGCATTGAGAGCCATGCAAATAGATACAAGCGGTACATTGTTCCTGTGCTATCTGTTCAGATGGATTTCTACGTTCGTGTTTTTGTTCGTATTTACACTTCTGCAAGTGCAATGAAAAATACTCCCCTTAAGCTCTCATATGTTTATCAGTGCACTGGTTGTGATTCTTTCCATCTCCAGCAGATTGGGAGAAGCGTCTCGAAGAACAACAGCATCAGGTATTTACCTGGTTTTGGTCCTTCTGTTCCTCAAGAGTGCAGTGATTGTGGGAAGAAATTCAACATGGGTGGGCCTATCTGGTCTGCTCCTATCCATGATCAAGAATGGGTTACTTCCATGCTAGCAGACGTGAAGTCTATGAAGGACTCATATCCTGCATATGATCGTATATCTGCTGTATTGACAACAATTTCAGAGGAATTGATGGATGTTCCCTTATTTTTGAGTCTACACAACCTCTGTGCAACATTGAAGTGCACATCTCCATCAGCTGTAAACTTCCGTTCTGCAGTGATTAATGCAGGATATCGCATATCGGGCACTCATGTGAATCCTTTAGGACTGAAATCAGATGCTCCCATGGATGTAATTTGGGATATTATGCGTTGCTGGGTCAAAAACCACCCGGTGAAACCTCAACCAGAAGATCAGCCAGGAAGTGTCATTCTTGCTAAGGAACCGGTGCTTCAAGCTAATTTTGCTAGAGCTGTGGCATCTCTTAGCAAGGCTCAAGCAAAGAAAGTTGCACGTTTCCTTCCAAATCCTGAAAGACATTGGGGCCCCAAGCTGAGAGCAGGTCGTCAAATCACTAGTAAGCACATCTCTCTACTGGGCGCAGAGGCAGTAAACGGAGCTCTTAGCCATACAGATTCTGAAGAGCCTAATGCAAAACGCCCCAATGTAGAAGAAGTTGCTCTTAGCCATACG

Coding sequence (CDS)

ATGGCGACAGATCTTAGTGAATTCACCATCATCAAGGAGGGAGAGGCTGAGATTCTCATGCACGCGAAGAATGAAGTCTTTTTCAATAAGACCCAGGTTAACAACAGAGACATATCCATTGCTGTCCTTAGGGCATTTATATCAAAACGCAAACAGGAGCACGAAGCAAAACTATCAAGAAGAACAAAAGCCGGGCAAAAGATTTCTGAAAATAATGTTTCTGGACCTGTTGTGGAAGAAACACCTAATGAATCTGCCACAAATGATGAAAAGTCAAATACAGATCGTGATGCTCATGAAGAAACACCGGATGAATGTGGAGTTCCAAAGTTGTCAGAAGAACAAAAAGAGATCATCGAGGAAAAAGAACAAAGAGATCCCAAGCCTCTTAGAGTTCTCGAGGCCTTGTCGGCTTCAGGGCTAAGAGCCTTAAGATATGCTCGTGAAGTAGAGGGCGTTGGTCAAGTTGTGGCGCTAGACAATGATAAAGCATCAGTGGAAGCTTGTCGAAGAAACATCAAGTTTAATGGTTCAGTAGCATGTGCGAAGGTGGAATCACATCTTACTGATGCTCGTGTTTATATGCTCACTCACCCAAAAGAATTTGATATGGTTGATCTGGATCCTTATGGTTCACCTTCTGTTTTCTTGGACTCTGCTGTTCAATCAGTTGTCGATGGTGGTATGCTGATGTGCACAGCAACTGATATGGCGGTGCTTTGTGGGGGAAATGGAGAAGTTTGCTATTCAAAATATGGTTCCTATCCTTTGAGAGGGAAATATTGCCACGAAATGGCTTTAAGAATCCTCCTTGCCTGCATTGAGAGCCATGCAAATAGATACAAGCGGTACATTGTTCCTGTGCTATCTGTTCAGATGGATTTCTACGTTCGTGTTTTTGTTCGTATTTACACTTCTGCAAGTGCAATGAAAAATACTCCCCTTAAGCTCTCATATGTTTATCAGTGCACTGGTTGTGATTCTTTCCATCTCCAGCAGATTGGGAGAAGCGTCTCGAAGAACAACAGCATCAGGTATTTACCTGGTTTTGGTCCTTCTGTTCCTCAAGAGTGCAGTGATTGTGGGAAGAAATTCAACATGGGTGGGCCTATCTGGTCTGCTCCTATCCATGATCAAGAATGGGTTACTTCCATGCTAGCAGACGTGAAGTCTATGAAGGACTCATATCCTGCATATGATCGTATATCTGCTGTATTGACAACAATTTCAGAGGAATTGATGGATGTTCCCTTATTTTTGAGTCTACACAACCTCTGTGCAACATTGAAGTGCACATCTCCATCAGCTGTAAACTTCCGTTCTGCAGTGATTAATGCAGGATATCGCATATCGGGCACTCATGTGAATCCTTTAGGACTGAAATCAGATGCTCCCATGGATGTAATTTGGGATATTATGCGTTGCTGGGTCAAAAACCACCCGGTGAAACCTCAACCAGAAGATCAGCCAGGAAGTGTCATTCTTGCTAAGGAACCGGTGCTTCAAGCTAATTTTGCTAGAGCTGTGGCATCTCTTAGCAAGGCTCAAGCAAAGAAAGTTGCACGTTTCCTTCCAAATCCTGAAAGACATTGGGGCCCCAAGCTGAGAGCAGGTCGTCAAATCACTAGTAAGCACATCTCTCTACTGGGCGCAGAGGCAGTAAACGGAGCTCTTAGCCATACAGATTCTGAAGAGCCTAATGCAAAACGCCCCAATGTAGAAGAAGTTGCTCTTAGCCATACG

Protein sequence

MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRAFISKRKQEHEAKLSRRTKAGQKISENNVSGPVVEETPNESATNDEKSNTDRDAHEETPDECGVPKLSEEQKEIIEEKEQRDPKPLRVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEACRRNIKFNGSVACAKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSVFLDSAVQSVVDGGMLMCTATDMAVLCGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVFVRIYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSIRYLPGFGPSVPQECSDCGKKFNMGGPIWSAPIHDQEWVTSMLADVKSMKDSYPAYDRISAVLTTISEELMDVPLFLSLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNHPVKPQPEDQPGSVILAKEPVLQANFARAVASLSKAQAKKVARFLPNPERHWGPKLRAGRQITSKHISLLGAEAVNGALSHTDSEEPNAKRPNVEEVALSHT
Homology
BLAST of MS003287 vs. NCBI nr
Match: XP_022134106.1 (probable tRNA (guanine(26)-N(2))-dimethyltransferase 2 [Momordica charantia])

HSP 1 Score: 1152.1 bits (2979), Expect = 0.0e+00
Identity = 579/581 (99.66%), Postives = 580/581 (99.83%), Query Frame = 0

Query: 1   MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRAFISKRKQEHEAKLSR 60
           MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRAFISKRKQEHEAKLSR
Sbjct: 1   MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRAFISKRKQEHEAKLSR 60

Query: 61  RTKAGQKISENNVSGPVVEETPNESATNDEKSNTDRDAHEETPDECGVPKLSEEQKEIIE 120
           RTKAGQKISENNVSGPVVEETPNESATNDEKSNTDRDAHEETPDECGVPKLSEEQKEIIE
Sbjct: 61  RTKAGQKISENNVSGPVVEETPNESATNDEKSNTDRDAHEETPDECGVPKLSEEQKEIIE 120

Query: 121 EKEQRDPKPLRVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEACRRNIKFNGSVA 180
           EKEQRDPKPLRVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEACRRNIKFNGSVA
Sbjct: 121 EKEQRDPKPLRVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEACRRNIKFNGSVA 180

Query: 181 CAKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSVFLDSAVQSVVDGGMLMCTATDMAVL 240
           CAKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSVFLDSAVQSVVDGGMLMCTATDMAVL
Sbjct: 181 CAKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSVFLDSAVQSVVDGGMLMCTATDMAVL 240

Query: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300
           CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF
Sbjct: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300

Query: 301 VRIYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSIRYLPGFGPSVPQECSD 360
           VRIYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSIRYLPGFGPSVPQECSD
Sbjct: 301 VRIYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSIRYLPGFGPSVPQECSD 360

Query: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSMKDSYPAYDRISAVLTTISEELMDVPLFL 420
           CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSMKD+YPAYDRISAVLTTISEELMDVPLFL
Sbjct: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSMKDTYPAYDRISAVLTTISEELMDVPLFL 420

Query: 421 SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480
           SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH
Sbjct: 421 SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480

Query: 481 PVKPQPEDQPGSVILAKEPVLQANFARAVASLSKAQAKKVARFLPNPERHWGPKLRAGRQ 540
           PVKPQPEDQPGSVILAKEPVLQANFARAVASLSKAQAKKVARFLPNPERHWGPKLRAGRQ
Sbjct: 481 PVKPQPEDQPGSVILAKEPVLQANFARAVASLSKAQAKKVARFLPNPERHWGPKLRAGRQ 540

Query: 541 ITSKHISLLGAEAVNGALSHTDSEEPNAKRPNVEEVALSHT 582
           I SKHISLLGAEAVNGALSHTDSEEPNAKRPNVEEVALSHT
Sbjct: 541 IASKHISLLGAEAVNGALSHTDSEEPNAKRPNVEEVALSHT 581

BLAST of MS003287 vs. NCBI nr
Match: XP_038890035.1 (probable tRNA (guanine(26)-N(2))-dimethyltransferase 2 [Benincasa hispida])

HSP 1 Score: 1081.6 bits (2796), Expect = 0.0e+00
Identity = 540/575 (93.91%), Postives = 557/575 (96.87%), Query Frame = 0

Query: 1   MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRAFISKRKQEHEAKLSR 60
           MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLR FI+KRKQEHEAKLS+
Sbjct: 1   MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRLFITKRKQEHEAKLSK 60

Query: 61  RTKAGQKISENNVSGPVVEETPNESATNDEKSNTDRDAHEETPDECGVPKLSEEQKEIIE 120
           R K+GQK++ENN S  V+EE  NESATNDEK +TD  AHE+T +ECG P LSEEQKE+ E
Sbjct: 61  RAKSGQKVTENNGSETVMEEASNESATNDEKCHTDCGAHEKTSNECGSPMLSEEQKEVAE 120

Query: 121 EKEQRDPKPLRVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEACRRNIKFNGSVA 180
            KEQRDPKP+RVLEALSASGLRALRYAREVEG+GQVVALDNDKASVEACRRNIKFNGSVA
Sbjct: 121 GKEQRDPKPIRVLEALSASGLRALRYAREVEGIGQVVALDNDKASVEACRRNIKFNGSVA 180

Query: 181 CAKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSVFLDSAVQSVVDGGMLMCTATDMAVL 240
           CAKVESHLTDARVYMLTHPKEFDMVDLDPYGSPS+FLDSAVQSVVDGGMLMCTATDMAVL
Sbjct: 181 CAKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL 240

Query: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300
           CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF
Sbjct: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300

Query: 301 VRIYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSIRYLPGFGPSVPQECSD 360
           VRIYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNS+RYLPGFGPSV QECSD
Sbjct: 301 VRIYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSVRYLPGFGPSVAQECSD 360

Query: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSMKDSYPAYDRISAVLTTISEELMDVPLFL 420
           CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSMKDSYPAYDRISAVLTTISEELMDVPLFL
Sbjct: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSMKDSYPAYDRISAVLTTISEELMDVPLFL 420

Query: 421 SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480
           SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH
Sbjct: 421 SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480

Query: 481 PVKPQPEDQPGSVILAKEPVLQANFARAVASLSKAQAKKVARFLPNPERHWGPKLRAGRQ 540
           PVK QPEDQPGS+ILAKEPVL+ANFARAVASLSKAQAKK+ARFLPNPERHWGPKLRAGRQ
Sbjct: 481 PVKAQPEDQPGSIILAKEPVLEANFARAVASLSKAQAKKIARFLPNPERHWGPKLRAGRQ 540

Query: 541 ITSKHISLLGAEAVNGALSHTDSEEPNAKRPNVEE 576
           ITSKHISLLGAEAVNGALSHTDSEEPNAKRP VEE
Sbjct: 541 ITSKHISLLGAEAVNGALSHTDSEEPNAKRPKVEE 575

BLAST of MS003287 vs. NCBI nr
Match: XP_011656015.1 (probable tRNA (guanine(26)-N(2))-dimethyltransferase 2 [Cucumis sativus] >KGN52527.1 hypothetical protein Csa_008279 [Cucumis sativus])

HSP 1 Score: 1073.2 bits (2774), Expect = 8.9e-310
Identity = 537/575 (93.39%), Postives = 555/575 (96.52%), Query Frame = 0

Query: 1   MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRAFISKRKQEHEAKLSR 60
           MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLR+FI+KRKQEHEAKLS+
Sbjct: 1   MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRSFIAKRKQEHEAKLSK 60

Query: 61  RTKAGQKISENNVSGPVVEETPNESATNDEKSNTDRDAHEETPDECGVPKLSEEQKEIIE 120
           R+K+GQK+SENN S    EE  NESATNDEK +TD DAHE+  +ECG P LSEEQKEI+E
Sbjct: 61  RSKSGQKLSENNGSETATEEACNESATNDEKCDTDCDAHEKPSNECGSPMLSEEQKEIVE 120

Query: 121 EKEQRDPKPLRVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEACRRNIKFNGSVA 180
            KEQR PKP+RVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEACRRNIKFNGSVA
Sbjct: 121 GKEQRGPKPIRVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEACRRNIKFNGSVA 180

Query: 181 CAKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSVFLDSAVQSVVDGGMLMCTATDMAVL 240
           C+KVESHLTDARVYMLTHPKEFDMVDLDPYGSPS+FLDSAVQSVVDGGMLMCTATDMAVL
Sbjct: 181 CSKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL 240

Query: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300
           CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF
Sbjct: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300

Query: 301 VRIYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSIRYLPGFGPSVPQECSD 360
           VR+YTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNS+RYLPGFGPSV QECSD
Sbjct: 301 VRVYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSVRYLPGFGPSVAQECSD 360

Query: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSMKDSYPAYDRISAVLTTISEELMDVPLFL 420
           CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSMKDSYPAYDRISAVLTTISEELMDVPLFL
Sbjct: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSMKDSYPAYDRISAVLTTISEELMDVPLFL 420

Query: 421 SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480
           SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH
Sbjct: 421 SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480

Query: 481 PVKPQPEDQPGSVILAKEPVLQANFARAVASLSKAQAKKVARFLPNPERHWGPKLRAGRQ 540
           PVK QPEDQ GS+ILAKEPVL+ANFARAVASLSKAQAKK+ARFLPNPERHWGPKLRAGRQ
Sbjct: 481 PVKTQPEDQAGSIILAKEPVLEANFARAVASLSKAQAKKIARFLPNPERHWGPKLRAGRQ 540

Query: 541 ITSKHISLLGAEAVNGALSHTDSEEPNAKRPNVEE 576
           ITSKHISLLGAEAVNGALSHTDSEEPNAKR  VEE
Sbjct: 541 ITSKHISLLGAEAVNGALSHTDSEEPNAKRSKVEE 575

BLAST of MS003287 vs. NCBI nr
Match: XP_008459398.1 (PREDICTED: probable tRNA (guanine(26)-N(2))-dimethyltransferase 2 [Cucumis melo])

HSP 1 Score: 1070.1 bits (2766), Expect = 6.7e-309
Identity = 535/575 (93.04%), Postives = 554/575 (96.35%), Query Frame = 0

Query: 1   MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRAFISKRKQEHEAKLSR 60
           MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLR+FI+KRKQEHEAKLS+
Sbjct: 1   MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRSFIAKRKQEHEAKLSK 60

Query: 61  RTKAGQKISENNVSGPVVEETPNESATNDEKSNTDRDAHEETPDECGVPKLSEEQKEIIE 120
           R+K+GQK+SENN S    EE  N SATN+EK +TD DAHE+  +ECG P LSEEQKEI+E
Sbjct: 61  RSKSGQKLSENNGSETATEEAFNGSATNEEKCHTDCDAHEKPSNECGSPMLSEEQKEIVE 120

Query: 121 EKEQRDPKPLRVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEACRRNIKFNGSVA 180
            KEQR PKP+RVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEAC+RNIKFNGSVA
Sbjct: 121 GKEQRGPKPIRVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEACQRNIKFNGSVA 180

Query: 181 CAKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSVFLDSAVQSVVDGGMLMCTATDMAVL 240
           C KVESHLTDARVYMLTHPKEFDMVDLDPYGSPS+FLDSAVQSVVDGGMLMCTATDMAVL
Sbjct: 181 CTKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL 240

Query: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300
           CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF
Sbjct: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300

Query: 301 VRIYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSIRYLPGFGPSVPQECSD 360
           VR+YTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNS+RYLPGFGPSV QECSD
Sbjct: 301 VRVYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSVRYLPGFGPSVAQECSD 360

Query: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSMKDSYPAYDRISAVLTTISEELMDVPLFL 420
           CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSMKDSYPAYDRISAVLTTISEEL DVPLFL
Sbjct: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSMKDSYPAYDRISAVLTTISEELNDVPLFL 420

Query: 421 SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480
           SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH
Sbjct: 421 SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480

Query: 481 PVKPQPEDQPGSVILAKEPVLQANFARAVASLSKAQAKKVARFLPNPERHWGPKLRAGRQ 540
           PVK QPEDQPGS+ILAKEPVL+ANFARAVASLSKAQAKK+ARFLPNPERHWGPKLRAGRQ
Sbjct: 481 PVKAQPEDQPGSIILAKEPVLEANFARAVASLSKAQAKKIARFLPNPERHWGPKLRAGRQ 540

Query: 541 ITSKHISLLGAEAVNGALSHTDSEEPNAKRPNVEE 576
           ITSKHISLLGAEAVNGALSHTDSEEPNAKRP VEE
Sbjct: 541 ITSKHISLLGAEAVNGALSHTDSEEPNAKRPKVEE 575

BLAST of MS003287 vs. NCBI nr
Match: KAA0039424.1 (putative tRNA (guanine(26)-N(2))-dimethyltransferase 2 [Cucumis melo var. makuwa] >TYK00612.1 putative tRNA (guanine(26)-N(2))-dimethyltransferase 2 [Cucumis melo var. makuwa])

HSP 1 Score: 1067.8 bits (2760), Expect = 3.4e-308
Identity = 534/575 (92.87%), Postives = 552/575 (96.00%), Query Frame = 0

Query: 1   MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRAFISKRKQEHEAKLSR 60
           M TDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLR+FI+KRKQEHEAKLS+
Sbjct: 1   MTTDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRSFIAKRKQEHEAKLSK 60

Query: 61  RTKAGQKISENNVSGPVVEETPNESATNDEKSNTDRDAHEETPDECGVPKLSEEQKEIIE 120
           R+K+GQK+SENN S    EE  N SATN+EK +TD DAHE+   ECG P LSEEQKEI+E
Sbjct: 61  RSKSGQKLSENNGSETATEEAFNGSATNEEKCHTDCDAHEKPSTECGSPMLSEEQKEIVE 120

Query: 121 EKEQRDPKPLRVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEACRRNIKFNGSVA 180
            KEQR PKP+RVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEAC+RNIKFNGSVA
Sbjct: 121 GKEQRGPKPIRVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEACQRNIKFNGSVA 180

Query: 181 CAKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSVFLDSAVQSVVDGGMLMCTATDMAVL 240
           C KVESHLTDARVYMLTHPKEFDMVDLDPYGSPS+FLDSAVQSVVDGGMLMCTATDMAVL
Sbjct: 181 CTKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL 240

Query: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300
           CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF
Sbjct: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300

Query: 301 VRIYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSIRYLPGFGPSVPQECSD 360
           VR+YTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNS+RYLPGFGPSV QECSD
Sbjct: 301 VRVYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSVRYLPGFGPSVAQECSD 360

Query: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSMKDSYPAYDRISAVLTTISEELMDVPLFL 420
           CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSMKDSYPAYDRISAVLTTISEEL DVPLFL
Sbjct: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSMKDSYPAYDRISAVLTTISEELNDVPLFL 420

Query: 421 SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480
           SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH
Sbjct: 421 SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480

Query: 481 PVKPQPEDQPGSVILAKEPVLQANFARAVASLSKAQAKKVARFLPNPERHWGPKLRAGRQ 540
           PVK QPEDQPGS+ILAKEPVL+ANFARAVASLSKAQAKK+ARFLPNPERHWGPKLRAGRQ
Sbjct: 481 PVKAQPEDQPGSIILAKEPVLEANFARAVASLSKAQAKKIARFLPNPERHWGPKLRAGRQ 540

Query: 541 ITSKHISLLGAEAVNGALSHTDSEEPNAKRPNVEE 576
           ITSKHISLLGAEAVNGALSHTDSEEPNAKRP VEE
Sbjct: 541 ITSKHISLLGAEAVNGALSHTDSEEPNAKRPKVEE 575

BLAST of MS003287 vs. ExPASy Swiss-Prot
Match: Q9SRU7 (Probable tRNA (guanine(26)-N(2))-dimethyltransferase 2 OS=Arabidopsis thaliana OX=3702 GN=At3g02320 PE=2 SV=3)

HSP 1 Score: 871.7 bits (2251), Expect = 4.7e-252
Identity = 431/564 (76.42%), Postives = 484/564 (85.82%), Query Frame = 0

Query: 1   MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRAFISKRKQEHEAKLSR 60
           M TDL+++T+IKEGEAEILMH KN+VFFNK QVNNRD+SIAVLR F+SKRKQEHEAK S+
Sbjct: 1   METDLNDYTVIKEGEAEILMHKKNQVFFNKAQVNNRDMSIAVLREFLSKRKQEHEAKSSK 60

Query: 61  RTKAGQKISENNVSGPVVEETPNESATNDEKSNTDRDAHEETPDECGVPKLSEEQKEIIE 120
           RT+   K+ E + S    EETP+E+  N        +   E   E G   +S++  +  E
Sbjct: 61  RTRPASKVIEKDASEASKEETPSENGMN--------NGDHEVASEDGPSSVSKDPAKTTE 120

Query: 121 EKEQRDPKPLRVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEACRRNIKFNGSVA 180
               R+PKP +VLEALSASGLRALRYARE+EG+GQVVALDND ASVEAC+RNIKFNGSVA
Sbjct: 121 RFAPREPKPPKVLEALSASGLRALRYAREIEGIGQVVALDNDLASVEACQRNIKFNGSVA 180

Query: 181 CAKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSVFLDSAVQSVVDGGMLMCTATDMAVL 240
            +KVESH TDARV+MLTHPKEFD+VDLDPYGSPS+FLDSA+QSV DGG+LMCTATDMAVL
Sbjct: 181 ISKVESHHTDARVHMLTHPKEFDVVDLDPYGSPSIFLDSAIQSVTDGGLLMCTATDMAVL 240

Query: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300
           CGGNGEVCYSKYGSYPLR KYCHEMALRILLA IESHANRYKRYIVPVLSVQMDFYVRVF
Sbjct: 241 CGGNGEVCYSKYGSYPLRAKYCHEMALRILLASIESHANRYKRYIVPVLSVQMDFYVRVF 300

Query: 301 VRIYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSIRYLPGFGPSVPQECSD 360
           VR+YTSASAMKNTPLKLSYVYQC GCDSFHLQ +GRS+ KNNS+RYLP  GP V Q+C+ 
Sbjct: 301 VRVYTSASAMKNTPLKLSYVYQCIGCDSFHLQPVGRSLPKNNSVRYLPAIGPVVKQDCNH 360

Query: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSMKDSYPAYDRISAVLTTISEELMDVPLFL 420
           CGKK+NMGGPIWSAP+HD EWVTS+L  VKSMKD YPAYDRISAVLTT+SEEL+DVPLFL
Sbjct: 361 CGKKYNMGGPIWSAPMHDPEWVTSILNSVKSMKDRYPAYDRISAVLTTVSEELLDVPLFL 420

Query: 421 SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480
           SLHNLCATLKC SPSA  FRSAVINA YRISGTHVNPLG+K+DAPM+VIWDIMRCWVKNH
Sbjct: 421 SLHNLCATLKCISPSAAMFRSAVINANYRISGTHVNPLGMKTDAPMEVIWDIMRCWVKNH 480

Query: 481 PVKPQPEDQPGSVILAKEPVLQANFARAVASLSKAQAKKVARFLPNPERHWGPKLRAGRQ 540
           P+K Q  +QPGSVIL+KEP  + +F+R + SLSKAQAKKVARFLPNPE+HWGPKLRAGRQ
Sbjct: 481 PIKAQSPEQPGSVILSKEPSHEVDFSRHIGSLSKAQAKKVARFLPNPEKHWGPKLRAGRQ 540

Query: 541 ITSKHISLLGAEAVNGALSHTDSE 565
           ITSKH+SL+G EAVNG LS    E
Sbjct: 541 ITSKHVSLIGHEAVNGHLSQHHEE 556

BLAST of MS003287 vs. ExPASy Swiss-Prot
Match: Q9LFU5 (Probable tRNA (guanine(26)-N(2))-dimethyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=At5g15810 PE=2 SV=3)

HSP 1 Score: 860.5 bits (2222), Expect = 1.1e-248
Identity = 432/575 (75.13%), Postives = 493/575 (85.74%), Query Frame = 0

Query: 1   MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRAFISKRKQEHEAKLSR 60
           M TDL+++T+IKEGEAE+LMH KN+VFFNK QVNNRD+SIAVLRAFI KRKQEHEA LS+
Sbjct: 1   METDLNDYTVIKEGEAEVLMHKKNQVFFNKAQVNNRDMSIAVLRAFIIKRKQEHEAMLSK 60

Query: 61  RTKAGQKISENNVSGPVVEETPNESATNDEKSNTDRDAHEETPDECGVPKLSEEQKEIIE 120
           R ++  K+ E +VS    EETP E+  ++ K+N +   HE T  +   PK   E  +   
Sbjct: 61  RARSSGKVVEKDVSETSKEETPTENGDDNGKTNGE---HEVTTQD--GPK---EAAKTAY 120

Query: 121 EKEQRDPKPLRVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEACRRNIKFNGSVA 180
           E  +R+ KP RVLEALSASGLRALRYAREVEG+GQVVALDND ASVEAC+RNIKFNG ++
Sbjct: 121 ESARRELKPPRVLEALSASGLRALRYAREVEGIGQVVALDNDPASVEACQRNIKFNGLMS 180

Query: 181 CAKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSVFLDSAVQSVVDGGMLMCTATDMAVL 240
            +KVESHLTDARV+ML+HPK+FD+VDLDPYG+PS+FLDSAVQSV DGG+LMCTATDMAVL
Sbjct: 181 TSKVESHLTDARVHMLSHPKDFDVVDLDPYGAPSIFLDSAVQSVADGGLLMCTATDMAVL 240

Query: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300
           CG NGEVCYSKYGSYPL+GKYCHEMALRILLA IESHANRYKRYIVPVLSVQMDFYVRVF
Sbjct: 241 CGANGEVCYSKYGSYPLKGKYCHEMALRILLASIESHANRYKRYIVPVLSVQMDFYVRVF 300

Query: 301 VRIYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSIRYLPGFGPSVPQECSD 360
           VR+YTSASAMKNTPLKLSYVYQC GCDSFHLQ +GRS+ KNNS+RYLPG GP VPQ+C+ 
Sbjct: 301 VRVYTSASAMKNTPLKLSYVYQCIGCDSFHLQSVGRSLPKNNSVRYLPGVGPVVPQDCTH 360

Query: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSMKDSYPAYDRISAVLTTISEELMDVPLFL 420
           CGKK+NMGGPIWSAPIHDQEWV S+L  VKSMKD YPAYDRI AVLTTISEEL DVPLFL
Sbjct: 361 CGKKYNMGGPIWSAPIHDQEWVNSILNGVKSMKDRYPAYDRICAVLTTISEELPDVPLFL 420

Query: 421 SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480
           SLH+L ATLKCTSPSA  FRSAVINA YR+SG+HVNPLG+K+DAPM++IWDIMRCWVKNH
Sbjct: 421 SLHSLSATLKCTSPSAALFRSAVINAKYRVSGSHVNPLGIKTDAPMEIIWDIMRCWVKNH 480

Query: 481 PVKPQPEDQPGSVILAKEPVLQANFARAVASLSKAQAKKVARFLPNPERHWGPKLRAGRQ 540
           P+KPQ  + PGSVIL+KEP  QA+F+R V SLSKAQAKKVARFLPNPE+HWGPK+RAGR 
Sbjct: 481 PIKPQSPEHPGSVILSKEPSHQADFSRHVGSLSKAQAKKVARFLPNPEKHWGPKIRAGRT 540

Query: 541 ITSKHISLLGAEAVNGALSHTDSEEPNAKRPNVEE 576
           ITSKH+SLLG EAVNG L++   E  + +    EE
Sbjct: 541 ITSKHVSLLGHEAVNGHLNNNHKEAGDEEEEEEEE 567

BLAST of MS003287 vs. ExPASy Swiss-Prot
Match: Q3TX08 (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Mus musculus OX=10090 GN=Trmt1 PE=1 SV=2)

HSP 1 Score: 388.3 bits (996), Expect = 1.6e-106
Identity = 222/533 (41.65%), Postives = 321/533 (60.23%), Query Frame = 0

Query: 11  IKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRAFISKRKQEHEAKLSRRTKAGQKISE 70
           + EG A+I+  + NEVF+N  Q  NRD++ AV+  F    +    AK  +    G+K SE
Sbjct: 58  VTEGAAKIVFPSANEVFYNPVQEFNRDLTCAVITEF---ARIHLGAKGIQIKVPGEKDSE 117

Query: 71  NNVSGPVVEETPNESATNDEKSNTDRDAHEETPDECGVPKLSEEQKEIIEEKEQRDPKPL 130
                  V+ +  E  T  +  N    A  + P    V ++ EE               L
Sbjct: 118 K----IAVDLSDQEEETAGKNENL---APGDWPRTAAVGEICEE--------------GL 177

Query: 131 RVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEACRRNIKFNGSVACAKVESHLTD 190
           RVLE L+ASGLR++R+A EV G+  VVA D    +VE   RN++ NG      V+ +  D
Sbjct: 178 RVLEGLAASGLRSIRFALEVPGLQSVVANDASARAVELMHRNVELNG--VAHLVQPNQAD 237

Query: 191 ARVYMLTH---PKEFDMVDLDPYGSPSVFLDSAVQSVVDGGMLMCTATDMAVLCGGNGEV 250
           AR+ M  H   P+ FD++DLDPYGSP+ FLD+AVQ+V DGG+L  T TDMAVL G +GE 
Sbjct: 238 ARMLMYQHQKAPERFDVIDLDPYGSPAPFLDAAVQAVSDGGLLCVTCTDMAVLAGNSGET 297

Query: 251 CYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVFVRIYTSA 310
           CYSKYG+  L+ + CHEMALRI+L  ++ HAN Y+RYIVP+LS+  DFY+RVFVR++T  
Sbjct: 298 CYSKYGAMALKSRACHEMALRIVLHSLDLHANCYQRYIVPLLSISADFYIRVFVRVFTGQ 357

Query: 311 SAMKNTPLKLSYVYQCTGCDSFHLQQIGR-SVSKNNSIRYLPGFGPSVPQECSDCGKKFN 370
           + +K++  K + V+QC GC +F+LQ++G+ S      I++    GP V  EC  CG++  
Sbjct: 358 AKVKSSASKQALVFQCVGCGAFYLQRLGKASGDPGGRIKFSAACGPPVTPECEHCGQRHQ 417

Query: 371 MGGPIWSAPIHDQEWVTSMLADVKSMKDSYPAYDRISAVLTTISEELMDVPLFLSLHNLC 430
           +GGP+W+ PIHD ++V  +L  V +    +    RI  VL+ ++EEL DVPL+ +L  L 
Sbjct: 418 LGGPMWAEPIHDLDFVGRVLDAVTTNPGRFHTSMRIQGVLSVVTEELPDVPLYYTLDQLS 477

Query: 431 ATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNHPVKPQ- 490
           +T+ C +P  +  RSA+++AG+R+S +H     +K+DAP + +WDIMRCW K  PVK + 
Sbjct: 478 STIHCNTPRLLQLRSALLHAGFRVSLSHACKNAVKTDAPPEALWDIMRCWEKECPVKRER 537

Query: 491 -PEDQPGSVILAKEPVLQANFARAVASLSKAQAKKVARFLPNPERHWGPKLRA 538
             E  P   ILA EP L+ANF     +   ++ + + RF  NPE +WGP+ RA
Sbjct: 538 LSESSPAFRILAVEPRLKANFNIREDANPSSRQRGLKRFQANPEANWGPRPRA 564

BLAST of MS003287 vs. ExPASy Swiss-Prot
Match: Q9NXH9 (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Homo sapiens OX=9606 GN=TRMT1 PE=1 SV=1)

HSP 1 Score: 379.8 bits (974), Expect = 5.6e-104
Identity = 215/536 (40.11%), Postives = 315/536 (58.77%), Query Frame = 0

Query: 9   TIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRAFISKRKQEHEAKLSRRTKAGQ-K 68
           T + EG A+I   + NEVF+N  Q  NRD++ AV+  F         A++    K  Q K
Sbjct: 55  TTVTEGAAKIAFPSANEVFYNPVQEFNRDLTCAVITEF---------ARIQLGAKGIQIK 114

Query: 69  ISENNVSGPVVEETPNESATNDEKSNTDRDAHEETPDECGVPKLSEEQKEIIEEKEQRDP 128
           +     +  VV +   +     E   ++  A  + P    V ++ EE             
Sbjct: 115 VPGEKDTQKVVVDLSEQEEEKVELKESENLASGDQPRTAAVGEICEE------------- 174

Query: 129 KPLRVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEACRRNIKFNGSVACAKVESH 188
             L VLE L+ASGLR++R+A EV G+  VVA D    +V+  RRN++ N       V+  
Sbjct: 175 -GLHVLEGLAASGLRSIRFALEVPGLRSVVANDASTRAVDLIRRNVQLND--VAHLVQPS 234

Query: 189 LTDARVYMLTHPK---EFDMVDLDPYGSPSVFLDSAVQSVVDGGMLMCTATDMAVLCGGN 248
             DAR+ M  H +    FD++DLDPYGSP+ FLD+AVQ+V +GG+L  T TDMAVL G +
Sbjct: 235 QADARMLMYQHQRVSERFDVIDLDPYGSPATFLDAAVQAVSEGGLLCVTCTDMAVLAGNS 294

Query: 249 GEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVFVRIY 308
           GE CYSKYG+  L+ + CHEMALRI+L  ++  AN Y+R++VP+LS+  DFYVRVFVR++
Sbjct: 295 GETCYSKYGAMALKSRACHEMALRIVLHSLDLRANCYQRFVVPLLSISADFYVRVFVRVF 354

Query: 309 TSASAMKNTPLKLSYVYQCTGCDSFHLQQIGR-SVSKNNSIRYLPGFGPSVPQECSDCGK 368
           T  + +K +  K + V+QC GC +FHLQ++G+ S   +   ++    GP V  EC  CG+
Sbjct: 355 TGQAKVKASASKQALVFQCVGCGAFHLQRLGKASGVPSGRAKFSAACGPPVTPECEHCGQ 414

Query: 369 KFNMGGPIWSAPIHDQEWVTSMLADVKSMKDSYPAYDRISAVLTTISEELMDVPLFLSLH 428
           +  +GGP+W+ PIHD ++V  +L  V +    +   +RI  VL+ I+EEL DVPL+ +L 
Sbjct: 415 RHQLGGPMWAEPIHDLDFVGRVLEAVSANPGRFHTSERIRGVLSVITEELPDVPLYYTLD 474

Query: 429 NLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNHPVK 488
            L +T+ C +PS +  RSA+++A +R+S +H     +K+DAP   +WDIMRCW K  PVK
Sbjct: 475 QLSSTIHCNTPSLLQLRSALLHADFRVSLSHACKNAVKTDAPASALWDIMRCWEKECPVK 534

Query: 489 PQ--PEDQPGSVILAKEPVLQANFARAVASLSKAQAKKVARFLPNPERHWGPKLRA 538
            +   E  P   IL+ EP LQANF     +   ++ + + RF  NPE +WGP+ RA
Sbjct: 535 RERLSETSPAFRILSVEPRLQANFTIREDANPSSRQRGLKRFQANPEANWGPRPRA 565

BLAST of MS003287 vs. ExPASy Swiss-Prot
Match: Q9VK89 (Probable tRNA (guanine(26)-N(2))-dimethyltransferase OS=Drosophila melanogaster OX=7227 GN=CG6388 PE=2 SV=1)

HSP 1 Score: 375.6 bits (963), Expect = 1.0e-102
Identity = 219/540 (40.56%), Postives = 306/540 (56.67%), Query Frame = 0

Query: 10  IIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRAFISKRKQEHEAKLSRRTKAGQKIS 69
           +I+E  AEI+  +   VF+N  Q  NRD+SIA L  +  +  +E   K  ++ +   K  
Sbjct: 19  VIRERNAEIV--SGGNVFYNPVQEFNRDLSIAALNVYRQRLTKERSEKALKKQRKKVKEQ 78

Query: 70  ENNVSGPVVEETPNESATNDEKSNTDRDAHEETPDECGVPKLSEEQKEIIEEKEQRDPKP 129
           E+  + PV E+ P                                    + E   R    
Sbjct: 79  EDEKTTPVPEDPP------------------------------------VYEAGTRYEDG 138

Query: 130 LRVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEACRRNIKFNGSVACAKVESHLT 189
           LR+LEAL+A+GLR++RYA+E+ GV Q+VA D  + +V +   NI+ N      +      
Sbjct: 139 LRILEALAATGLRSIRYAQEIAGVRQIVANDLSRQAVASINTNIRHNKVEELIEPSHSDA 198

Query: 190 DARVYMLTHP-KEFDMVDLDPYGSPSVFLDSAVQSVVDGGMLMCTATDMAVLCGGNGEVC 249
              +Y+ T P K FD VDLDPYG P+ FLD A+Q +VDGG+L+ TATDMAVL G   E C
Sbjct: 199 MTLMYLSTQPEKRFDAVDLDPYGCPNRFLDGAMQCLVDGGLLLVTATDMAVLAGNAPEAC 258

Query: 250 YSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVFVRIYTSAS 309
           Y KYGS PLR K CHEMALRILL CIESHANRY +YI P+LS+  DFY+R+FVR+Y   +
Sbjct: 259 YVKYGSVPLRMKCCHEMALRILLHCIESHANRYGKYIEPLLSISADFYIRIFVRVYVGQA 318

Query: 310 AMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNN-------SIRYLPGFGPSVPQECSDC 369
             K +  K S++YQCTGC++F LQ +G  ++K N        +++    GP+V  +C  C
Sbjct: 319 QCKLSMSKQSWIYQCTGCETFTLQPLG--ITKPNPTAGNPQQLKFGIPTGPAVNSQCEHC 378

Query: 370 GKKFNMGGPIWSAPIHDQEWVTSMLADV-KSMKDSYPAYDRISAVLTTISEELMDVPLFL 429
           G + ++GGPIWSAPIH+ E+V  +L  V ++   S     RI  VL+ + EEL DVPL+ 
Sbjct: 379 GHRHHLGGPIWSAPIHNPEFVQDLLTAVQETTLQSLGTQRRIVGVLSMVQEELQDVPLYY 438

Query: 430 SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 489
           +   LC  LK      + FRSA+++AGYR+S +H +   LK++AP  V+WDI+R W K H
Sbjct: 439 TPDKLCCVLKLEIVPMLKFRSAILHAGYRVSYSHASKNSLKTNAPPAVLWDILRSWSKRH 498

Query: 490 PVKPQP--EDQPGSVILAKEPVLQANFARAVASLS-KAQAKKVARFLPNPERHWGPKLRA 538
           PV P+      P + IL+KE      F       + K++   ++RF  NP  HWGP  RA
Sbjct: 499 PVNPERMIPGSPLAAILSKECTAVYEFDELHPEANPKSRKSALSRFQENPTPHWGPGTRA 518

BLAST of MS003287 vs. ExPASy TrEMBL
Match: A0A6J1BX10 (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Momordica charantia OX=3673 GN=LOC111006460 PE=3 SV=1)

HSP 1 Score: 1152.1 bits (2979), Expect = 0.0e+00
Identity = 579/581 (99.66%), Postives = 580/581 (99.83%), Query Frame = 0

Query: 1   MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRAFISKRKQEHEAKLSR 60
           MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRAFISKRKQEHEAKLSR
Sbjct: 1   MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRAFISKRKQEHEAKLSR 60

Query: 61  RTKAGQKISENNVSGPVVEETPNESATNDEKSNTDRDAHEETPDECGVPKLSEEQKEIIE 120
           RTKAGQKISENNVSGPVVEETPNESATNDEKSNTDRDAHEETPDECGVPKLSEEQKEIIE
Sbjct: 61  RTKAGQKISENNVSGPVVEETPNESATNDEKSNTDRDAHEETPDECGVPKLSEEQKEIIE 120

Query: 121 EKEQRDPKPLRVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEACRRNIKFNGSVA 180
           EKEQRDPKPLRVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEACRRNIKFNGSVA
Sbjct: 121 EKEQRDPKPLRVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEACRRNIKFNGSVA 180

Query: 181 CAKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSVFLDSAVQSVVDGGMLMCTATDMAVL 240
           CAKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSVFLDSAVQSVVDGGMLMCTATDMAVL
Sbjct: 181 CAKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSVFLDSAVQSVVDGGMLMCTATDMAVL 240

Query: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300
           CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF
Sbjct: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300

Query: 301 VRIYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSIRYLPGFGPSVPQECSD 360
           VRIYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSIRYLPGFGPSVPQECSD
Sbjct: 301 VRIYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSIRYLPGFGPSVPQECSD 360

Query: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSMKDSYPAYDRISAVLTTISEELMDVPLFL 420
           CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSMKD+YPAYDRISAVLTTISEELMDVPLFL
Sbjct: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSMKDTYPAYDRISAVLTTISEELMDVPLFL 420

Query: 421 SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480
           SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH
Sbjct: 421 SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480

Query: 481 PVKPQPEDQPGSVILAKEPVLQANFARAVASLSKAQAKKVARFLPNPERHWGPKLRAGRQ 540
           PVKPQPEDQPGSVILAKEPVLQANFARAVASLSKAQAKKVARFLPNPERHWGPKLRAGRQ
Sbjct: 481 PVKPQPEDQPGSVILAKEPVLQANFARAVASLSKAQAKKVARFLPNPERHWGPKLRAGRQ 540

Query: 541 ITSKHISLLGAEAVNGALSHTDSEEPNAKRPNVEEVALSHT 582
           I SKHISLLGAEAVNGALSHTDSEEPNAKRPNVEEVALSHT
Sbjct: 541 IASKHISLLGAEAVNGALSHTDSEEPNAKRPNVEEVALSHT 581

BLAST of MS003287 vs. ExPASy TrEMBL
Match: A0A0A0KVU4 (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucumis sativus OX=3659 GN=Csa_5G641600 PE=3 SV=1)

HSP 1 Score: 1073.2 bits (2774), Expect = 4.3e-310
Identity = 537/575 (93.39%), Postives = 555/575 (96.52%), Query Frame = 0

Query: 1   MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRAFISKRKQEHEAKLSR 60
           MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLR+FI+KRKQEHEAKLS+
Sbjct: 1   MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRSFIAKRKQEHEAKLSK 60

Query: 61  RTKAGQKISENNVSGPVVEETPNESATNDEKSNTDRDAHEETPDECGVPKLSEEQKEIIE 120
           R+K+GQK+SENN S    EE  NESATNDEK +TD DAHE+  +ECG P LSEEQKEI+E
Sbjct: 61  RSKSGQKLSENNGSETATEEACNESATNDEKCDTDCDAHEKPSNECGSPMLSEEQKEIVE 120

Query: 121 EKEQRDPKPLRVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEACRRNIKFNGSVA 180
            KEQR PKP+RVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEACRRNIKFNGSVA
Sbjct: 121 GKEQRGPKPIRVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEACRRNIKFNGSVA 180

Query: 181 CAKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSVFLDSAVQSVVDGGMLMCTATDMAVL 240
           C+KVESHLTDARVYMLTHPKEFDMVDLDPYGSPS+FLDSAVQSVVDGGMLMCTATDMAVL
Sbjct: 181 CSKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL 240

Query: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300
           CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF
Sbjct: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300

Query: 301 VRIYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSIRYLPGFGPSVPQECSD 360
           VR+YTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNS+RYLPGFGPSV QECSD
Sbjct: 301 VRVYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSVRYLPGFGPSVAQECSD 360

Query: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSMKDSYPAYDRISAVLTTISEELMDVPLFL 420
           CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSMKDSYPAYDRISAVLTTISEELMDVPLFL
Sbjct: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSMKDSYPAYDRISAVLTTISEELMDVPLFL 420

Query: 421 SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480
           SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH
Sbjct: 421 SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480

Query: 481 PVKPQPEDQPGSVILAKEPVLQANFARAVASLSKAQAKKVARFLPNPERHWGPKLRAGRQ 540
           PVK QPEDQ GS+ILAKEPVL+ANFARAVASLSKAQAKK+ARFLPNPERHWGPKLRAGRQ
Sbjct: 481 PVKTQPEDQAGSIILAKEPVLEANFARAVASLSKAQAKKIARFLPNPERHWGPKLRAGRQ 540

Query: 541 ITSKHISLLGAEAVNGALSHTDSEEPNAKRPNVEE 576
           ITSKHISLLGAEAVNGALSHTDSEEPNAKR  VEE
Sbjct: 541 ITSKHISLLGAEAVNGALSHTDSEEPNAKRSKVEE 575

BLAST of MS003287 vs. ExPASy TrEMBL
Match: A0A1S3CA23 (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucumis melo OX=3656 GN=LOC103498546 PE=3 SV=1)

HSP 1 Score: 1070.1 bits (2766), Expect = 3.2e-309
Identity = 535/575 (93.04%), Postives = 554/575 (96.35%), Query Frame = 0

Query: 1   MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRAFISKRKQEHEAKLSR 60
           MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLR+FI+KRKQEHEAKLS+
Sbjct: 1   MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRSFIAKRKQEHEAKLSK 60

Query: 61  RTKAGQKISENNVSGPVVEETPNESATNDEKSNTDRDAHEETPDECGVPKLSEEQKEIIE 120
           R+K+GQK+SENN S    EE  N SATN+EK +TD DAHE+  +ECG P LSEEQKEI+E
Sbjct: 61  RSKSGQKLSENNGSETATEEAFNGSATNEEKCHTDCDAHEKPSNECGSPMLSEEQKEIVE 120

Query: 121 EKEQRDPKPLRVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEACRRNIKFNGSVA 180
            KEQR PKP+RVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEAC+RNIKFNGSVA
Sbjct: 121 GKEQRGPKPIRVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEACQRNIKFNGSVA 180

Query: 181 CAKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSVFLDSAVQSVVDGGMLMCTATDMAVL 240
           C KVESHLTDARVYMLTHPKEFDMVDLDPYGSPS+FLDSAVQSVVDGGMLMCTATDMAVL
Sbjct: 181 CTKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL 240

Query: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300
           CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF
Sbjct: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300

Query: 301 VRIYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSIRYLPGFGPSVPQECSD 360
           VR+YTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNS+RYLPGFGPSV QECSD
Sbjct: 301 VRVYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSVRYLPGFGPSVAQECSD 360

Query: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSMKDSYPAYDRISAVLTTISEELMDVPLFL 420
           CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSMKDSYPAYDRISAVLTTISEEL DVPLFL
Sbjct: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSMKDSYPAYDRISAVLTTISEELNDVPLFL 420

Query: 421 SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480
           SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH
Sbjct: 421 SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480

Query: 481 PVKPQPEDQPGSVILAKEPVLQANFARAVASLSKAQAKKVARFLPNPERHWGPKLRAGRQ 540
           PVK QPEDQPGS+ILAKEPVL+ANFARAVASLSKAQAKK+ARFLPNPERHWGPKLRAGRQ
Sbjct: 481 PVKAQPEDQPGSIILAKEPVLEANFARAVASLSKAQAKKIARFLPNPERHWGPKLRAGRQ 540

Query: 541 ITSKHISLLGAEAVNGALSHTDSEEPNAKRPNVEE 576
           ITSKHISLLGAEAVNGALSHTDSEEPNAKRP VEE
Sbjct: 541 ITSKHISLLGAEAVNGALSHTDSEEPNAKRPKVEE 575

BLAST of MS003287 vs. ExPASy TrEMBL
Match: A0A5A7T7H3 (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold169G002310 PE=3 SV=1)

HSP 1 Score: 1067.8 bits (2760), Expect = 1.6e-308
Identity = 534/575 (92.87%), Postives = 552/575 (96.00%), Query Frame = 0

Query: 1   MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRAFISKRKQEHEAKLSR 60
           M TDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLR+FI+KRKQEHEAKLS+
Sbjct: 1   MTTDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRSFIAKRKQEHEAKLSK 60

Query: 61  RTKAGQKISENNVSGPVVEETPNESATNDEKSNTDRDAHEETPDECGVPKLSEEQKEIIE 120
           R+K+GQK+SENN S    EE  N SATN+EK +TD DAHE+   ECG P LSEEQKEI+E
Sbjct: 61  RSKSGQKLSENNGSETATEEAFNGSATNEEKCHTDCDAHEKPSTECGSPMLSEEQKEIVE 120

Query: 121 EKEQRDPKPLRVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEACRRNIKFNGSVA 180
            KEQR PKP+RVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEAC+RNIKFNGSVA
Sbjct: 121 GKEQRGPKPIRVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEACQRNIKFNGSVA 180

Query: 181 CAKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSVFLDSAVQSVVDGGMLMCTATDMAVL 240
           C KVESHLTDARVYMLTHPKEFDMVDLDPYGSPS+FLDSAVQSVVDGGMLMCTATDMAVL
Sbjct: 181 CTKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL 240

Query: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300
           CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF
Sbjct: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300

Query: 301 VRIYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSIRYLPGFGPSVPQECSD 360
           VR+YTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNS+RYLPGFGPSV QECSD
Sbjct: 301 VRVYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSVRYLPGFGPSVAQECSD 360

Query: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSMKDSYPAYDRISAVLTTISEELMDVPLFL 420
           CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSMKDSYPAYDRISAVLTTISEEL DVPLFL
Sbjct: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSMKDSYPAYDRISAVLTTISEELNDVPLFL 420

Query: 421 SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480
           SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH
Sbjct: 421 SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480

Query: 481 PVKPQPEDQPGSVILAKEPVLQANFARAVASLSKAQAKKVARFLPNPERHWGPKLRAGRQ 540
           PVK QPEDQPGS+ILAKEPVL+ANFARAVASLSKAQAKK+ARFLPNPERHWGPKLRAGRQ
Sbjct: 481 PVKAQPEDQPGSIILAKEPVLEANFARAVASLSKAQAKKIARFLPNPERHWGPKLRAGRQ 540

Query: 541 ITSKHISLLGAEAVNGALSHTDSEEPNAKRPNVEE 576
           ITSKHISLLGAEAVNGALSHTDSEEPNAKRP VEE
Sbjct: 541 ITSKHISLLGAEAVNGALSHTDSEEPNAKRPKVEE 575

BLAST of MS003287 vs. ExPASy TrEMBL
Match: A0A6J1GSN6 (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111456770 PE=3 SV=1)

HSP 1 Score: 1064.7 bits (2752), Expect = 1.4e-307
Identity = 531/575 (92.35%), Postives = 551/575 (95.83%), Query Frame = 0

Query: 1   MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRAFISKRKQEHEAKLSR 60
           MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRD+SIAVLRAFISKRKQEHE K+SR
Sbjct: 1   MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDLSIAVLRAFISKRKQEHETKISR 60

Query: 61  RTKAGQKISENNVSGPVVEETPNESATNDEKSNTDRDAHEETPDECGVPKLSEEQKEIIE 120
           R K+ QKISENN S  V+EE  NESATNDEK NTD DAHE+  +ECG P LSEEQKE + 
Sbjct: 61  RAKSAQKISENNGSEIVMEEASNESATNDEKCNTDCDAHEKLSNECGSPVLSEEQKENVA 120

Query: 121 EKEQRDPKPLRVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEACRRNIKFNGSVA 180
            KE+RD KP+RVLEALSASGLRALRYAREVEG+GQVVALDNDKASVEACRRNIKFNGS+A
Sbjct: 121 GKERRDAKPIRVLEALSASGLRALRYAREVEGIGQVVALDNDKASVEACRRNIKFNGSIA 180

Query: 181 CAKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSVFLDSAVQSVVDGGMLMCTATDMAVL 240
           CAKVESHLTDARVYMLTHP EFDMVDLDPYGSPS+FLDSAVQSVVDGGMLMCTATDMAVL
Sbjct: 181 CAKVESHLTDARVYMLTHPNEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL 240

Query: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300
           CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF
Sbjct: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300

Query: 301 VRIYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSIRYLPGFGPSVPQECSD 360
           VR+YTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNS+RYLPGFGPSVPQECSD
Sbjct: 301 VRVYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSVRYLPGFGPSVPQECSD 360

Query: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSMKDSYPAYDRISAVLTTISEELMDVPLFL 420
           CGKKFNMGGPIWSAPIHDQEWV+SMLADVKSMKD+YPAYDRISAVLTTISEELMDVPLFL
Sbjct: 361 CGKKFNMGGPIWSAPIHDQEWVSSMLADVKSMKDTYPAYDRISAVLTTISEELMDVPLFL 420

Query: 421 SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480
           SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPM VIWDIMRCWVKNH
Sbjct: 421 SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMSVIWDIMRCWVKNH 480

Query: 481 PVKPQPEDQPGSVILAKEPVLQANFARAVASLSKAQAKKVARFLPNPERHWGPKLRAGRQ 540
           PVK QPEDQPGS+ILAKEPVL+ANFARA+ASLSKAQAKKVARFLPNPERHWGPKLRAGRQ
Sbjct: 481 PVKAQPEDQPGSIILAKEPVLEANFARAIASLSKAQAKKVARFLPNPERHWGPKLRAGRQ 540

Query: 541 ITSKHISLLGAEAVNGALSHTDSEEPNAKRPNVEE 576
           ITSKHISLLGA+AVNGALSH DSEEPNAKR  VEE
Sbjct: 541 ITSKHISLLGADAVNGALSHADSEEPNAKRSKVEE 575

BLAST of MS003287 vs. TAIR 10
Match: AT3G02320.1 (N2,N2-dimethylguanosine tRNA methyltransferase )

HSP 1 Score: 871.7 bits (2251), Expect = 3.3e-253
Identity = 431/564 (76.42%), Postives = 484/564 (85.82%), Query Frame = 0

Query: 1   MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRAFISKRKQEHEAKLSR 60
           M TDL+++T+IKEGEAEILMH KN+VFFNK QVNNRD+SIAVLR F+SKRKQEHEAK S+
Sbjct: 1   METDLNDYTVIKEGEAEILMHKKNQVFFNKAQVNNRDMSIAVLREFLSKRKQEHEAKSSK 60

Query: 61  RTKAGQKISENNVSGPVVEETPNESATNDEKSNTDRDAHEETPDECGVPKLSEEQKEIIE 120
           RT+   K+ E + S    EETP+E+  N        +   E   E G   +S++  +  E
Sbjct: 61  RTRPASKVIEKDASEASKEETPSENGMN--------NGDHEVASEDGPSSVSKDPAKTTE 120

Query: 121 EKEQRDPKPLRVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEACRRNIKFNGSVA 180
               R+PKP +VLEALSASGLRALRYARE+EG+GQVVALDND ASVEAC+RNIKFNGSVA
Sbjct: 121 RFAPREPKPPKVLEALSASGLRALRYAREIEGIGQVVALDNDLASVEACQRNIKFNGSVA 180

Query: 181 CAKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSVFLDSAVQSVVDGGMLMCTATDMAVL 240
            +KVESH TDARV+MLTHPKEFD+VDLDPYGSPS+FLDSA+QSV DGG+LMCTATDMAVL
Sbjct: 181 ISKVESHHTDARVHMLTHPKEFDVVDLDPYGSPSIFLDSAIQSVTDGGLLMCTATDMAVL 240

Query: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300
           CGGNGEVCYSKYGSYPLR KYCHEMALRILLA IESHANRYKRYIVPVLSVQMDFYVRVF
Sbjct: 241 CGGNGEVCYSKYGSYPLRAKYCHEMALRILLASIESHANRYKRYIVPVLSVQMDFYVRVF 300

Query: 301 VRIYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSIRYLPGFGPSVPQECSD 360
           VR+YTSASAMKNTPLKLSYVYQC GCDSFHLQ +GRS+ KNNS+RYLP  GP V Q+C+ 
Sbjct: 301 VRVYTSASAMKNTPLKLSYVYQCIGCDSFHLQPVGRSLPKNNSVRYLPAIGPVVKQDCNH 360

Query: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSMKDSYPAYDRISAVLTTISEELMDVPLFL 420
           CGKK+NMGGPIWSAP+HD EWVTS+L  VKSMKD YPAYDRISAVLTT+SEEL+DVPLFL
Sbjct: 361 CGKKYNMGGPIWSAPMHDPEWVTSILNSVKSMKDRYPAYDRISAVLTTVSEELLDVPLFL 420

Query: 421 SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480
           SLHNLCATLKC SPSA  FRSAVINA YRISGTHVNPLG+K+DAPM+VIWDIMRCWVKNH
Sbjct: 421 SLHNLCATLKCISPSAAMFRSAVINANYRISGTHVNPLGMKTDAPMEVIWDIMRCWVKNH 480

Query: 481 PVKPQPEDQPGSVILAKEPVLQANFARAVASLSKAQAKKVARFLPNPERHWGPKLRAGRQ 540
           P+K Q  +QPGSVIL+KEP  + +F+R + SLSKAQAKKVARFLPNPE+HWGPKLRAGRQ
Sbjct: 481 PIKAQSPEQPGSVILSKEPSHEVDFSRHIGSLSKAQAKKVARFLPNPEKHWGPKLRAGRQ 540

Query: 541 ITSKHISLLGAEAVNGALSHTDSE 565
           ITSKH+SL+G EAVNG LS    E
Sbjct: 541 ITSKHVSLIGHEAVNGHLSQHHEE 556

BLAST of MS003287 vs. TAIR 10
Match: AT5G15810.1 (N2,N2-dimethylguanosine tRNA methyltransferase )

HSP 1 Score: 860.5 bits (2222), Expect = 7.6e-250
Identity = 432/575 (75.13%), Postives = 493/575 (85.74%), Query Frame = 0

Query: 1   MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRAFISKRKQEHEAKLSR 60
           M TDL+++T+IKEGEAE+LMH KN+VFFNK QVNNRD+SIAVLRAFI KRKQEHEA LS+
Sbjct: 99  METDLNDYTVIKEGEAEVLMHKKNQVFFNKAQVNNRDMSIAVLRAFIIKRKQEHEAMLSK 158

Query: 61  RTKAGQKISENNVSGPVVEETPNESATNDEKSNTDRDAHEETPDECGVPKLSEEQKEIIE 120
           R ++  K+ E +VS    EETP E+  ++ K+N +   HE T  +   PK   E  +   
Sbjct: 159 RARSSGKVVEKDVSETSKEETPTENGDDNGKTNGE---HEVTTQD--GPK---EAAKTAY 218

Query: 121 EKEQRDPKPLRVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEACRRNIKFNGSVA 180
           E  +R+ KP RVLEALSASGLRALRYAREVEG+GQVVALDND ASVEAC+RNIKFNG ++
Sbjct: 219 ESARRELKPPRVLEALSASGLRALRYAREVEGIGQVVALDNDPASVEACQRNIKFNGLMS 278

Query: 181 CAKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSVFLDSAVQSVVDGGMLMCTATDMAVL 240
            +KVESHLTDARV+ML+HPK+FD+VDLDPYG+PS+FLDSAVQSV DGG+LMCTATDMAVL
Sbjct: 279 TSKVESHLTDARVHMLSHPKDFDVVDLDPYGAPSIFLDSAVQSVADGGLLMCTATDMAVL 338

Query: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300
           CG NGEVCYSKYGSYPL+GKYCHEMALRILLA IESHANRYKRYIVPVLSVQMDFYVRVF
Sbjct: 339 CGANGEVCYSKYGSYPLKGKYCHEMALRILLASIESHANRYKRYIVPVLSVQMDFYVRVF 398

Query: 301 VRIYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSIRYLPGFGPSVPQECSD 360
           VR+YTSASAMKNTPLKLSYVYQC GCDSFHLQ +GRS+ KNNS+RYLPG GP VPQ+C+ 
Sbjct: 399 VRVYTSASAMKNTPLKLSYVYQCIGCDSFHLQSVGRSLPKNNSVRYLPGVGPVVPQDCTH 458

Query: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSMKDSYPAYDRISAVLTTISEELMDVPLFL 420
           CGKK+NMGGPIWSAPIHDQEWV S+L  VKSMKD YPAYDRI AVLTTISEEL DVPLFL
Sbjct: 459 CGKKYNMGGPIWSAPIHDQEWVNSILNGVKSMKDRYPAYDRICAVLTTISEELPDVPLFL 518

Query: 421 SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480
           SLH+L ATLKCTSPSA  FRSAVINA YR+SG+HVNPLG+K+DAPM++IWDIMRCWVKNH
Sbjct: 519 SLHSLSATLKCTSPSAALFRSAVINAKYRVSGSHVNPLGIKTDAPMEIIWDIMRCWVKNH 578

Query: 481 PVKPQPEDQPGSVILAKEPVLQANFARAVASLSKAQAKKVARFLPNPERHWGPKLRAGRQ 540
           P+KPQ  + PGSVIL+KEP  QA+F+R V SLSKAQAKKVARFLPNPE+HWGPK+RAGR 
Sbjct: 579 PIKPQSPEHPGSVILSKEPSHQADFSRHVGSLSKAQAKKVARFLPNPEKHWGPKIRAGRT 638

Query: 541 ITSKHISLLGAEAVNGALSHTDSEEPNAKRPNVEE 576
           ITSKH+SLLG EAVNG L++   E  + +    EE
Sbjct: 639 ITSKHVSLLGHEAVNGHLNNNHKEAGDEEEEEEEE 665

BLAST of MS003287 vs. TAIR 10
Match: AT3G56330.1 (N2,N2-dimethylguanosine tRNA methyltransferase )

HSP 1 Score: 95.9 bits (237), Expect = 1.1e-19
Identity = 91/360 (25.28%), Postives = 152/360 (42.22%), Query Frame = 0

Query: 124 QRDPKPLRVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEACRRNIKFNGSVACAK 183
           +R    LRVL+A+   G+R+LRY  E E    V+A D +  +    RR I  N     +K
Sbjct: 84  KRSNGSLRVLDAMCGCGIRSLRYLVEAE-ADFVMANDANDDN----RRVITDN----LSK 143

Query: 184 VESHLTDARVYMLTH-------------PKEFDMVDLDPYGSPSVFLDSAVQSVVDGGML 243
           VE    D R +++TH                FDM+D+D +GS S FL  A  ++  GG+L
Sbjct: 144 VERGTGDERRWVVTHMLANKAMIERYMVADFFDMIDIDSFGSDSSFLRDAFNALRLGGLL 203

Query: 244 MCTATDMAVLCGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLS 303
             T+TD     G       + YG++     + +E+ LR+L+      A     ++ P+ S
Sbjct: 204 YLTSTDGYSSGGHRPYNSLAAYGAFIRPMPFGNEIGLRMLIGGAVREAALLGYHVTPLFS 263

Query: 304 VQMDFYVRVFVRIYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSIRYLPGF 363
               ++  VF  +        +      +V  C  C   H            ++R+    
Sbjct: 264 -YYSYHGPVFRVMLRVHRGKLHEDRNYGFVTHCNLCGHSH------------TLRFDELG 323

Query: 364 GPSVPQECSDCGKKFNMGGPIWSAPIHDQEWVTSMLADVKS----MKDSYPAYDRISAVL 423
               P   +       + GP+W  P+HD  +VT ML   K      + +    D++ +++
Sbjct: 324 LMGCPCSDTKASSSLVVSGPMWLGPLHDASYVTEMLELAKEWGWVSEGTGMDLDKLLSIM 383

Query: 424 TTISEELMDVPLFLSLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPM 467
              S+  +  P +  +  + +  K  SP      SA++  GY  S +H+ P  LK+D PM
Sbjct: 384 IEESDPRLP-PGYTKMDEMASRAKMNSPPLKKMMSALVKEGYAASRSHIIPNALKTDCPM 420

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022134106.10.0e+0099.66probable tRNA (guanine(26)-N(2))-dimethyltransferase 2 [Momordica charantia][more]
XP_038890035.10.0e+0093.91probable tRNA (guanine(26)-N(2))-dimethyltransferase 2 [Benincasa hispida][more]
XP_011656015.18.9e-31093.39probable tRNA (guanine(26)-N(2))-dimethyltransferase 2 [Cucumis sativus] >KGN525... [more]
XP_008459398.16.7e-30993.04PREDICTED: probable tRNA (guanine(26)-N(2))-dimethyltransferase 2 [Cucumis melo][more]
KAA0039424.13.4e-30892.87putative tRNA (guanine(26)-N(2))-dimethyltransferase 2 [Cucumis melo var. makuwa... [more]
Match NameE-valueIdentityDescription
Q9SRU74.7e-25276.42Probable tRNA (guanine(26)-N(2))-dimethyltransferase 2 OS=Arabidopsis thaliana O... [more]
Q9LFU51.1e-24875.13Probable tRNA (guanine(26)-N(2))-dimethyltransferase 1 OS=Arabidopsis thaliana O... [more]
Q3TX081.6e-10641.65tRNA (guanine(26)-N(2))-dimethyltransferase OS=Mus musculus OX=10090 GN=Trmt1 PE... [more]
Q9NXH95.6e-10440.11tRNA (guanine(26)-N(2))-dimethyltransferase OS=Homo sapiens OX=9606 GN=TRMT1 PE=... [more]
Q9VK891.0e-10240.56Probable tRNA (guanine(26)-N(2))-dimethyltransferase OS=Drosophila melanogaster ... [more]
Match NameE-valueIdentityDescription
A0A6J1BX100.0e+0099.66tRNA (guanine(26)-N(2))-dimethyltransferase OS=Momordica charantia OX=3673 GN=LO... [more]
A0A0A0KVU44.3e-31093.39tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucumis sativus OX=3659 GN=Csa_5G... [more]
A0A1S3CA233.2e-30993.04tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucumis melo OX=3656 GN=LOC103498... [more]
A0A5A7T7H31.6e-30892.87tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucumis melo var. makuwa OX=11946... [more]
A0A6J1GSN61.4e-30792.35tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucurbita moschata OX=3662 GN=LOC... [more]
Match NameE-valueIdentityDescription
AT3G02320.13.3e-25376.42N2,N2-dimethylguanosine tRNA methyltransferase [more]
AT5G15810.17.6e-25075.13N2,N2-dimethylguanosine tRNA methyltransferase [more]
AT3G56330.11.1e-1925.28N2,N2-dimethylguanosine tRNA methyltransferase [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR042296tRNA methyltransferase, Trm1, C-terminalGENE3D3.30.56.70coord: 417..475
e-value: 2.1E-21
score: 77.4
NoneNo IPR availableGENE3D3.40.50.150Vaccinia Virus protein VP39coord: 8..415
e-value: 4.9E-125
score: 418.5
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 65..83
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 53..112
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 84..112
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 559..581
NoneNo IPR availablePANTHERPTHR10631:SF12TRNA (GUANINE(26)-N(2))-DIMETHYLTRANSFERASE 1-RELATEDcoord: 1..563
IPR002905tRNA methyltransferase, Trm1PFAMPF02005TRMcoord: 12..474
e-value: 2.7E-120
score: 402.0
IPR002905tRNA methyltransferase, Trm1TIGRFAMTIGR00308TIGR00308coord: 11..475
e-value: 4.0E-96
score: 320.6
IPR002905tRNA methyltransferase, Trm1PANTHERPTHR10631N 2 ,N 2 -DIMETHYLGUANOSINE TRNA METHYLTRANSFERASEcoord: 1..563
IPR002905tRNA methyltransferase, Trm1PROSITEPS51626SAM_MT_TRM1coord: 9..473
score: 139.433121
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILY53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 8..475

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS003287.1MS003287.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008033 tRNA processing
molecular_function GO:0003723 RNA binding
molecular_function GO:0004809 tRNA (guanine-N2-)-methyltransferase activity