Homology
BLAST of MS003287 vs. NCBI nr
Match:
XP_022134106.1 (probable tRNA (guanine(26)-N(2))-dimethyltransferase 2 [Momordica charantia])
HSP 1 Score: 1152.1 bits (2979), Expect = 0.0e+00
Identity = 579/581 (99.66%), Postives = 580/581 (99.83%), Query Frame = 0
Query: 1 MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRAFISKRKQEHEAKLSR 60
MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRAFISKRKQEHEAKLSR
Sbjct: 1 MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRAFISKRKQEHEAKLSR 60
Query: 61 RTKAGQKISENNVSGPVVEETPNESATNDEKSNTDRDAHEETPDECGVPKLSEEQKEIIE 120
RTKAGQKISENNVSGPVVEETPNESATNDEKSNTDRDAHEETPDECGVPKLSEEQKEIIE
Sbjct: 61 RTKAGQKISENNVSGPVVEETPNESATNDEKSNTDRDAHEETPDECGVPKLSEEQKEIIE 120
Query: 121 EKEQRDPKPLRVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEACRRNIKFNGSVA 180
EKEQRDPKPLRVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEACRRNIKFNGSVA
Sbjct: 121 EKEQRDPKPLRVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEACRRNIKFNGSVA 180
Query: 181 CAKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSVFLDSAVQSVVDGGMLMCTATDMAVL 240
CAKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSVFLDSAVQSVVDGGMLMCTATDMAVL
Sbjct: 181 CAKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSVFLDSAVQSVVDGGMLMCTATDMAVL 240
Query: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300
CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF
Sbjct: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300
Query: 301 VRIYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSIRYLPGFGPSVPQECSD 360
VRIYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSIRYLPGFGPSVPQECSD
Sbjct: 301 VRIYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSIRYLPGFGPSVPQECSD 360
Query: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSMKDSYPAYDRISAVLTTISEELMDVPLFL 420
CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSMKD+YPAYDRISAVLTTISEELMDVPLFL
Sbjct: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSMKDTYPAYDRISAVLTTISEELMDVPLFL 420
Query: 421 SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480
SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH
Sbjct: 421 SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480
Query: 481 PVKPQPEDQPGSVILAKEPVLQANFARAVASLSKAQAKKVARFLPNPERHWGPKLRAGRQ 540
PVKPQPEDQPGSVILAKEPVLQANFARAVASLSKAQAKKVARFLPNPERHWGPKLRAGRQ
Sbjct: 481 PVKPQPEDQPGSVILAKEPVLQANFARAVASLSKAQAKKVARFLPNPERHWGPKLRAGRQ 540
Query: 541 ITSKHISLLGAEAVNGALSHTDSEEPNAKRPNVEEVALSHT 582
I SKHISLLGAEAVNGALSHTDSEEPNAKRPNVEEVALSHT
Sbjct: 541 IASKHISLLGAEAVNGALSHTDSEEPNAKRPNVEEVALSHT 581
BLAST of MS003287 vs. NCBI nr
Match:
XP_038890035.1 (probable tRNA (guanine(26)-N(2))-dimethyltransferase 2 [Benincasa hispida])
HSP 1 Score: 1081.6 bits (2796), Expect = 0.0e+00
Identity = 540/575 (93.91%), Postives = 557/575 (96.87%), Query Frame = 0
Query: 1 MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRAFISKRKQEHEAKLSR 60
MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLR FI+KRKQEHEAKLS+
Sbjct: 1 MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRLFITKRKQEHEAKLSK 60
Query: 61 RTKAGQKISENNVSGPVVEETPNESATNDEKSNTDRDAHEETPDECGVPKLSEEQKEIIE 120
R K+GQK++ENN S V+EE NESATNDEK +TD AHE+T +ECG P LSEEQKE+ E
Sbjct: 61 RAKSGQKVTENNGSETVMEEASNESATNDEKCHTDCGAHEKTSNECGSPMLSEEQKEVAE 120
Query: 121 EKEQRDPKPLRVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEACRRNIKFNGSVA 180
KEQRDPKP+RVLEALSASGLRALRYAREVEG+GQVVALDNDKASVEACRRNIKFNGSVA
Sbjct: 121 GKEQRDPKPIRVLEALSASGLRALRYAREVEGIGQVVALDNDKASVEACRRNIKFNGSVA 180
Query: 181 CAKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSVFLDSAVQSVVDGGMLMCTATDMAVL 240
CAKVESHLTDARVYMLTHPKEFDMVDLDPYGSPS+FLDSAVQSVVDGGMLMCTATDMAVL
Sbjct: 181 CAKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL 240
Query: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300
CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF
Sbjct: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300
Query: 301 VRIYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSIRYLPGFGPSVPQECSD 360
VRIYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNS+RYLPGFGPSV QECSD
Sbjct: 301 VRIYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSVRYLPGFGPSVAQECSD 360
Query: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSMKDSYPAYDRISAVLTTISEELMDVPLFL 420
CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSMKDSYPAYDRISAVLTTISEELMDVPLFL
Sbjct: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSMKDSYPAYDRISAVLTTISEELMDVPLFL 420
Query: 421 SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480
SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH
Sbjct: 421 SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480
Query: 481 PVKPQPEDQPGSVILAKEPVLQANFARAVASLSKAQAKKVARFLPNPERHWGPKLRAGRQ 540
PVK QPEDQPGS+ILAKEPVL+ANFARAVASLSKAQAKK+ARFLPNPERHWGPKLRAGRQ
Sbjct: 481 PVKAQPEDQPGSIILAKEPVLEANFARAVASLSKAQAKKIARFLPNPERHWGPKLRAGRQ 540
Query: 541 ITSKHISLLGAEAVNGALSHTDSEEPNAKRPNVEE 576
ITSKHISLLGAEAVNGALSHTDSEEPNAKRP VEE
Sbjct: 541 ITSKHISLLGAEAVNGALSHTDSEEPNAKRPKVEE 575
BLAST of MS003287 vs. NCBI nr
Match:
XP_011656015.1 (probable tRNA (guanine(26)-N(2))-dimethyltransferase 2 [Cucumis sativus] >KGN52527.1 hypothetical protein Csa_008279 [Cucumis sativus])
HSP 1 Score: 1073.2 bits (2774), Expect = 8.9e-310
Identity = 537/575 (93.39%), Postives = 555/575 (96.52%), Query Frame = 0
Query: 1 MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRAFISKRKQEHEAKLSR 60
MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLR+FI+KRKQEHEAKLS+
Sbjct: 1 MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRSFIAKRKQEHEAKLSK 60
Query: 61 RTKAGQKISENNVSGPVVEETPNESATNDEKSNTDRDAHEETPDECGVPKLSEEQKEIIE 120
R+K+GQK+SENN S EE NESATNDEK +TD DAHE+ +ECG P LSEEQKEI+E
Sbjct: 61 RSKSGQKLSENNGSETATEEACNESATNDEKCDTDCDAHEKPSNECGSPMLSEEQKEIVE 120
Query: 121 EKEQRDPKPLRVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEACRRNIKFNGSVA 180
KEQR PKP+RVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEACRRNIKFNGSVA
Sbjct: 121 GKEQRGPKPIRVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEACRRNIKFNGSVA 180
Query: 181 CAKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSVFLDSAVQSVVDGGMLMCTATDMAVL 240
C+KVESHLTDARVYMLTHPKEFDMVDLDPYGSPS+FLDSAVQSVVDGGMLMCTATDMAVL
Sbjct: 181 CSKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL 240
Query: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300
CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF
Sbjct: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300
Query: 301 VRIYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSIRYLPGFGPSVPQECSD 360
VR+YTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNS+RYLPGFGPSV QECSD
Sbjct: 301 VRVYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSVRYLPGFGPSVAQECSD 360
Query: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSMKDSYPAYDRISAVLTTISEELMDVPLFL 420
CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSMKDSYPAYDRISAVLTTISEELMDVPLFL
Sbjct: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSMKDSYPAYDRISAVLTTISEELMDVPLFL 420
Query: 421 SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480
SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH
Sbjct: 421 SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480
Query: 481 PVKPQPEDQPGSVILAKEPVLQANFARAVASLSKAQAKKVARFLPNPERHWGPKLRAGRQ 540
PVK QPEDQ GS+ILAKEPVL+ANFARAVASLSKAQAKK+ARFLPNPERHWGPKLRAGRQ
Sbjct: 481 PVKTQPEDQAGSIILAKEPVLEANFARAVASLSKAQAKKIARFLPNPERHWGPKLRAGRQ 540
Query: 541 ITSKHISLLGAEAVNGALSHTDSEEPNAKRPNVEE 576
ITSKHISLLGAEAVNGALSHTDSEEPNAKR VEE
Sbjct: 541 ITSKHISLLGAEAVNGALSHTDSEEPNAKRSKVEE 575
BLAST of MS003287 vs. NCBI nr
Match:
XP_008459398.1 (PREDICTED: probable tRNA (guanine(26)-N(2))-dimethyltransferase 2 [Cucumis melo])
HSP 1 Score: 1070.1 bits (2766), Expect = 6.7e-309
Identity = 535/575 (93.04%), Postives = 554/575 (96.35%), Query Frame = 0
Query: 1 MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRAFISKRKQEHEAKLSR 60
MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLR+FI+KRKQEHEAKLS+
Sbjct: 1 MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRSFIAKRKQEHEAKLSK 60
Query: 61 RTKAGQKISENNVSGPVVEETPNESATNDEKSNTDRDAHEETPDECGVPKLSEEQKEIIE 120
R+K+GQK+SENN S EE N SATN+EK +TD DAHE+ +ECG P LSEEQKEI+E
Sbjct: 61 RSKSGQKLSENNGSETATEEAFNGSATNEEKCHTDCDAHEKPSNECGSPMLSEEQKEIVE 120
Query: 121 EKEQRDPKPLRVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEACRRNIKFNGSVA 180
KEQR PKP+RVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEAC+RNIKFNGSVA
Sbjct: 121 GKEQRGPKPIRVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEACQRNIKFNGSVA 180
Query: 181 CAKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSVFLDSAVQSVVDGGMLMCTATDMAVL 240
C KVESHLTDARVYMLTHPKEFDMVDLDPYGSPS+FLDSAVQSVVDGGMLMCTATDMAVL
Sbjct: 181 CTKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL 240
Query: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300
CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF
Sbjct: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300
Query: 301 VRIYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSIRYLPGFGPSVPQECSD 360
VR+YTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNS+RYLPGFGPSV QECSD
Sbjct: 301 VRVYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSVRYLPGFGPSVAQECSD 360
Query: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSMKDSYPAYDRISAVLTTISEELMDVPLFL 420
CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSMKDSYPAYDRISAVLTTISEEL DVPLFL
Sbjct: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSMKDSYPAYDRISAVLTTISEELNDVPLFL 420
Query: 421 SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480
SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH
Sbjct: 421 SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480
Query: 481 PVKPQPEDQPGSVILAKEPVLQANFARAVASLSKAQAKKVARFLPNPERHWGPKLRAGRQ 540
PVK QPEDQPGS+ILAKEPVL+ANFARAVASLSKAQAKK+ARFLPNPERHWGPKLRAGRQ
Sbjct: 481 PVKAQPEDQPGSIILAKEPVLEANFARAVASLSKAQAKKIARFLPNPERHWGPKLRAGRQ 540
Query: 541 ITSKHISLLGAEAVNGALSHTDSEEPNAKRPNVEE 576
ITSKHISLLGAEAVNGALSHTDSEEPNAKRP VEE
Sbjct: 541 ITSKHISLLGAEAVNGALSHTDSEEPNAKRPKVEE 575
BLAST of MS003287 vs. NCBI nr
Match:
KAA0039424.1 (putative tRNA (guanine(26)-N(2))-dimethyltransferase 2 [Cucumis melo var. makuwa] >TYK00612.1 putative tRNA (guanine(26)-N(2))-dimethyltransferase 2 [Cucumis melo var. makuwa])
HSP 1 Score: 1067.8 bits (2760), Expect = 3.4e-308
Identity = 534/575 (92.87%), Postives = 552/575 (96.00%), Query Frame = 0
Query: 1 MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRAFISKRKQEHEAKLSR 60
M TDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLR+FI+KRKQEHEAKLS+
Sbjct: 1 MTTDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRSFIAKRKQEHEAKLSK 60
Query: 61 RTKAGQKISENNVSGPVVEETPNESATNDEKSNTDRDAHEETPDECGVPKLSEEQKEIIE 120
R+K+GQK+SENN S EE N SATN+EK +TD DAHE+ ECG P LSEEQKEI+E
Sbjct: 61 RSKSGQKLSENNGSETATEEAFNGSATNEEKCHTDCDAHEKPSTECGSPMLSEEQKEIVE 120
Query: 121 EKEQRDPKPLRVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEACRRNIKFNGSVA 180
KEQR PKP+RVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEAC+RNIKFNGSVA
Sbjct: 121 GKEQRGPKPIRVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEACQRNIKFNGSVA 180
Query: 181 CAKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSVFLDSAVQSVVDGGMLMCTATDMAVL 240
C KVESHLTDARVYMLTHPKEFDMVDLDPYGSPS+FLDSAVQSVVDGGMLMCTATDMAVL
Sbjct: 181 CTKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL 240
Query: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300
CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF
Sbjct: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300
Query: 301 VRIYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSIRYLPGFGPSVPQECSD 360
VR+YTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNS+RYLPGFGPSV QECSD
Sbjct: 301 VRVYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSVRYLPGFGPSVAQECSD 360
Query: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSMKDSYPAYDRISAVLTTISEELMDVPLFL 420
CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSMKDSYPAYDRISAVLTTISEEL DVPLFL
Sbjct: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSMKDSYPAYDRISAVLTTISEELNDVPLFL 420
Query: 421 SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480
SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH
Sbjct: 421 SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480
Query: 481 PVKPQPEDQPGSVILAKEPVLQANFARAVASLSKAQAKKVARFLPNPERHWGPKLRAGRQ 540
PVK QPEDQPGS+ILAKEPVL+ANFARAVASLSKAQAKK+ARFLPNPERHWGPKLRAGRQ
Sbjct: 481 PVKAQPEDQPGSIILAKEPVLEANFARAVASLSKAQAKKIARFLPNPERHWGPKLRAGRQ 540
Query: 541 ITSKHISLLGAEAVNGALSHTDSEEPNAKRPNVEE 576
ITSKHISLLGAEAVNGALSHTDSEEPNAKRP VEE
Sbjct: 541 ITSKHISLLGAEAVNGALSHTDSEEPNAKRPKVEE 575
BLAST of MS003287 vs. ExPASy Swiss-Prot
Match:
Q9SRU7 (Probable tRNA (guanine(26)-N(2))-dimethyltransferase 2 OS=Arabidopsis thaliana OX=3702 GN=At3g02320 PE=2 SV=3)
HSP 1 Score: 871.7 bits (2251), Expect = 4.7e-252
Identity = 431/564 (76.42%), Postives = 484/564 (85.82%), Query Frame = 0
Query: 1 MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRAFISKRKQEHEAKLSR 60
M TDL+++T+IKEGEAEILMH KN+VFFNK QVNNRD+SIAVLR F+SKRKQEHEAK S+
Sbjct: 1 METDLNDYTVIKEGEAEILMHKKNQVFFNKAQVNNRDMSIAVLREFLSKRKQEHEAKSSK 60
Query: 61 RTKAGQKISENNVSGPVVEETPNESATNDEKSNTDRDAHEETPDECGVPKLSEEQKEIIE 120
RT+ K+ E + S EETP+E+ N + E E G +S++ + E
Sbjct: 61 RTRPASKVIEKDASEASKEETPSENGMN--------NGDHEVASEDGPSSVSKDPAKTTE 120
Query: 121 EKEQRDPKPLRVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEACRRNIKFNGSVA 180
R+PKP +VLEALSASGLRALRYARE+EG+GQVVALDND ASVEAC+RNIKFNGSVA
Sbjct: 121 RFAPREPKPPKVLEALSASGLRALRYAREIEGIGQVVALDNDLASVEACQRNIKFNGSVA 180
Query: 181 CAKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSVFLDSAVQSVVDGGMLMCTATDMAVL 240
+KVESH TDARV+MLTHPKEFD+VDLDPYGSPS+FLDSA+QSV DGG+LMCTATDMAVL
Sbjct: 181 ISKVESHHTDARVHMLTHPKEFDVVDLDPYGSPSIFLDSAIQSVTDGGLLMCTATDMAVL 240
Query: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300
CGGNGEVCYSKYGSYPLR KYCHEMALRILLA IESHANRYKRYIVPVLSVQMDFYVRVF
Sbjct: 241 CGGNGEVCYSKYGSYPLRAKYCHEMALRILLASIESHANRYKRYIVPVLSVQMDFYVRVF 300
Query: 301 VRIYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSIRYLPGFGPSVPQECSD 360
VR+YTSASAMKNTPLKLSYVYQC GCDSFHLQ +GRS+ KNNS+RYLP GP V Q+C+
Sbjct: 301 VRVYTSASAMKNTPLKLSYVYQCIGCDSFHLQPVGRSLPKNNSVRYLPAIGPVVKQDCNH 360
Query: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSMKDSYPAYDRISAVLTTISEELMDVPLFL 420
CGKK+NMGGPIWSAP+HD EWVTS+L VKSMKD YPAYDRISAVLTT+SEEL+DVPLFL
Sbjct: 361 CGKKYNMGGPIWSAPMHDPEWVTSILNSVKSMKDRYPAYDRISAVLTTVSEELLDVPLFL 420
Query: 421 SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480
SLHNLCATLKC SPSA FRSAVINA YRISGTHVNPLG+K+DAPM+VIWDIMRCWVKNH
Sbjct: 421 SLHNLCATLKCISPSAAMFRSAVINANYRISGTHVNPLGMKTDAPMEVIWDIMRCWVKNH 480
Query: 481 PVKPQPEDQPGSVILAKEPVLQANFARAVASLSKAQAKKVARFLPNPERHWGPKLRAGRQ 540
P+K Q +QPGSVIL+KEP + +F+R + SLSKAQAKKVARFLPNPE+HWGPKLRAGRQ
Sbjct: 481 PIKAQSPEQPGSVILSKEPSHEVDFSRHIGSLSKAQAKKVARFLPNPEKHWGPKLRAGRQ 540
Query: 541 ITSKHISLLGAEAVNGALSHTDSE 565
ITSKH+SL+G EAVNG LS E
Sbjct: 541 ITSKHVSLIGHEAVNGHLSQHHEE 556
BLAST of MS003287 vs. ExPASy Swiss-Prot
Match:
Q9LFU5 (Probable tRNA (guanine(26)-N(2))-dimethyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=At5g15810 PE=2 SV=3)
HSP 1 Score: 860.5 bits (2222), Expect = 1.1e-248
Identity = 432/575 (75.13%), Postives = 493/575 (85.74%), Query Frame = 0
Query: 1 MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRAFISKRKQEHEAKLSR 60
M TDL+++T+IKEGEAE+LMH KN+VFFNK QVNNRD+SIAVLRAFI KRKQEHEA LS+
Sbjct: 1 METDLNDYTVIKEGEAEVLMHKKNQVFFNKAQVNNRDMSIAVLRAFIIKRKQEHEAMLSK 60
Query: 61 RTKAGQKISENNVSGPVVEETPNESATNDEKSNTDRDAHEETPDECGVPKLSEEQKEIIE 120
R ++ K+ E +VS EETP E+ ++ K+N + HE T + PK E +
Sbjct: 61 RARSSGKVVEKDVSETSKEETPTENGDDNGKTNGE---HEVTTQD--GPK---EAAKTAY 120
Query: 121 EKEQRDPKPLRVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEACRRNIKFNGSVA 180
E +R+ KP RVLEALSASGLRALRYAREVEG+GQVVALDND ASVEAC+RNIKFNG ++
Sbjct: 121 ESARRELKPPRVLEALSASGLRALRYAREVEGIGQVVALDNDPASVEACQRNIKFNGLMS 180
Query: 181 CAKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSVFLDSAVQSVVDGGMLMCTATDMAVL 240
+KVESHLTDARV+ML+HPK+FD+VDLDPYG+PS+FLDSAVQSV DGG+LMCTATDMAVL
Sbjct: 181 TSKVESHLTDARVHMLSHPKDFDVVDLDPYGAPSIFLDSAVQSVADGGLLMCTATDMAVL 240
Query: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300
CG NGEVCYSKYGSYPL+GKYCHEMALRILLA IESHANRYKRYIVPVLSVQMDFYVRVF
Sbjct: 241 CGANGEVCYSKYGSYPLKGKYCHEMALRILLASIESHANRYKRYIVPVLSVQMDFYVRVF 300
Query: 301 VRIYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSIRYLPGFGPSVPQECSD 360
VR+YTSASAMKNTPLKLSYVYQC GCDSFHLQ +GRS+ KNNS+RYLPG GP VPQ+C+
Sbjct: 301 VRVYTSASAMKNTPLKLSYVYQCIGCDSFHLQSVGRSLPKNNSVRYLPGVGPVVPQDCTH 360
Query: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSMKDSYPAYDRISAVLTTISEELMDVPLFL 420
CGKK+NMGGPIWSAPIHDQEWV S+L VKSMKD YPAYDRI AVLTTISEEL DVPLFL
Sbjct: 361 CGKKYNMGGPIWSAPIHDQEWVNSILNGVKSMKDRYPAYDRICAVLTTISEELPDVPLFL 420
Query: 421 SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480
SLH+L ATLKCTSPSA FRSAVINA YR+SG+HVNPLG+K+DAPM++IWDIMRCWVKNH
Sbjct: 421 SLHSLSATLKCTSPSAALFRSAVINAKYRVSGSHVNPLGIKTDAPMEIIWDIMRCWVKNH 480
Query: 481 PVKPQPEDQPGSVILAKEPVLQANFARAVASLSKAQAKKVARFLPNPERHWGPKLRAGRQ 540
P+KPQ + PGSVIL+KEP QA+F+R V SLSKAQAKKVARFLPNPE+HWGPK+RAGR
Sbjct: 481 PIKPQSPEHPGSVILSKEPSHQADFSRHVGSLSKAQAKKVARFLPNPEKHWGPKIRAGRT 540
Query: 541 ITSKHISLLGAEAVNGALSHTDSEEPNAKRPNVEE 576
ITSKH+SLLG EAVNG L++ E + + EE
Sbjct: 541 ITSKHVSLLGHEAVNGHLNNNHKEAGDEEEEEEEE 567
BLAST of MS003287 vs. ExPASy Swiss-Prot
Match:
Q3TX08 (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Mus musculus OX=10090 GN=Trmt1 PE=1 SV=2)
HSP 1 Score: 388.3 bits (996), Expect = 1.6e-106
Identity = 222/533 (41.65%), Postives = 321/533 (60.23%), Query Frame = 0
Query: 11 IKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRAFISKRKQEHEAKLSRRTKAGQKISE 70
+ EG A+I+ + NEVF+N Q NRD++ AV+ F + AK + G+K SE
Sbjct: 58 VTEGAAKIVFPSANEVFYNPVQEFNRDLTCAVITEF---ARIHLGAKGIQIKVPGEKDSE 117
Query: 71 NNVSGPVVEETPNESATNDEKSNTDRDAHEETPDECGVPKLSEEQKEIIEEKEQRDPKPL 130
V+ + E T + N A + P V ++ EE L
Sbjct: 118 K----IAVDLSDQEEETAGKNENL---APGDWPRTAAVGEICEE--------------GL 177
Query: 131 RVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEACRRNIKFNGSVACAKVESHLTD 190
RVLE L+ASGLR++R+A EV G+ VVA D +VE RN++ NG V+ + D
Sbjct: 178 RVLEGLAASGLRSIRFALEVPGLQSVVANDASARAVELMHRNVELNG--VAHLVQPNQAD 237
Query: 191 ARVYMLTH---PKEFDMVDLDPYGSPSVFLDSAVQSVVDGGMLMCTATDMAVLCGGNGEV 250
AR+ M H P+ FD++DLDPYGSP+ FLD+AVQ+V DGG+L T TDMAVL G +GE
Sbjct: 238 ARMLMYQHQKAPERFDVIDLDPYGSPAPFLDAAVQAVSDGGLLCVTCTDMAVLAGNSGET 297
Query: 251 CYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVFVRIYTSA 310
CYSKYG+ L+ + CHEMALRI+L ++ HAN Y+RYIVP+LS+ DFY+RVFVR++T
Sbjct: 298 CYSKYGAMALKSRACHEMALRIVLHSLDLHANCYQRYIVPLLSISADFYIRVFVRVFTGQ 357
Query: 311 SAMKNTPLKLSYVYQCTGCDSFHLQQIGR-SVSKNNSIRYLPGFGPSVPQECSDCGKKFN 370
+ +K++ K + V+QC GC +F+LQ++G+ S I++ GP V EC CG++
Sbjct: 358 AKVKSSASKQALVFQCVGCGAFYLQRLGKASGDPGGRIKFSAACGPPVTPECEHCGQRHQ 417
Query: 371 MGGPIWSAPIHDQEWVTSMLADVKSMKDSYPAYDRISAVLTTISEELMDVPLFLSLHNLC 430
+GGP+W+ PIHD ++V +L V + + RI VL+ ++EEL DVPL+ +L L
Sbjct: 418 LGGPMWAEPIHDLDFVGRVLDAVTTNPGRFHTSMRIQGVLSVVTEELPDVPLYYTLDQLS 477
Query: 431 ATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNHPVKPQ- 490
+T+ C +P + RSA+++AG+R+S +H +K+DAP + +WDIMRCW K PVK +
Sbjct: 478 STIHCNTPRLLQLRSALLHAGFRVSLSHACKNAVKTDAPPEALWDIMRCWEKECPVKRER 537
Query: 491 -PEDQPGSVILAKEPVLQANFARAVASLSKAQAKKVARFLPNPERHWGPKLRA 538
E P ILA EP L+ANF + ++ + + RF NPE +WGP+ RA
Sbjct: 538 LSESSPAFRILAVEPRLKANFNIREDANPSSRQRGLKRFQANPEANWGPRPRA 564
BLAST of MS003287 vs. ExPASy Swiss-Prot
Match:
Q9NXH9 (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Homo sapiens OX=9606 GN=TRMT1 PE=1 SV=1)
HSP 1 Score: 379.8 bits (974), Expect = 5.6e-104
Identity = 215/536 (40.11%), Postives = 315/536 (58.77%), Query Frame = 0
Query: 9 TIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRAFISKRKQEHEAKLSRRTKAGQ-K 68
T + EG A+I + NEVF+N Q NRD++ AV+ F A++ K Q K
Sbjct: 55 TTVTEGAAKIAFPSANEVFYNPVQEFNRDLTCAVITEF---------ARIQLGAKGIQIK 114
Query: 69 ISENNVSGPVVEETPNESATNDEKSNTDRDAHEETPDECGVPKLSEEQKEIIEEKEQRDP 128
+ + VV + + E ++ A + P V ++ EE
Sbjct: 115 VPGEKDTQKVVVDLSEQEEEKVELKESENLASGDQPRTAAVGEICEE------------- 174
Query: 129 KPLRVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEACRRNIKFNGSVACAKVESH 188
L VLE L+ASGLR++R+A EV G+ VVA D +V+ RRN++ N V+
Sbjct: 175 -GLHVLEGLAASGLRSIRFALEVPGLRSVVANDASTRAVDLIRRNVQLND--VAHLVQPS 234
Query: 189 LTDARVYMLTHPK---EFDMVDLDPYGSPSVFLDSAVQSVVDGGMLMCTATDMAVLCGGN 248
DAR+ M H + FD++DLDPYGSP+ FLD+AVQ+V +GG+L T TDMAVL G +
Sbjct: 235 QADARMLMYQHQRVSERFDVIDLDPYGSPATFLDAAVQAVSEGGLLCVTCTDMAVLAGNS 294
Query: 249 GEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVFVRIY 308
GE CYSKYG+ L+ + CHEMALRI+L ++ AN Y+R++VP+LS+ DFYVRVFVR++
Sbjct: 295 GETCYSKYGAMALKSRACHEMALRIVLHSLDLRANCYQRFVVPLLSISADFYVRVFVRVF 354
Query: 309 TSASAMKNTPLKLSYVYQCTGCDSFHLQQIGR-SVSKNNSIRYLPGFGPSVPQECSDCGK 368
T + +K + K + V+QC GC +FHLQ++G+ S + ++ GP V EC CG+
Sbjct: 355 TGQAKVKASASKQALVFQCVGCGAFHLQRLGKASGVPSGRAKFSAACGPPVTPECEHCGQ 414
Query: 369 KFNMGGPIWSAPIHDQEWVTSMLADVKSMKDSYPAYDRISAVLTTISEELMDVPLFLSLH 428
+ +GGP+W+ PIHD ++V +L V + + +RI VL+ I+EEL DVPL+ +L
Sbjct: 415 RHQLGGPMWAEPIHDLDFVGRVLEAVSANPGRFHTSERIRGVLSVITEELPDVPLYYTLD 474
Query: 429 NLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNHPVK 488
L +T+ C +PS + RSA+++A +R+S +H +K+DAP +WDIMRCW K PVK
Sbjct: 475 QLSSTIHCNTPSLLQLRSALLHADFRVSLSHACKNAVKTDAPASALWDIMRCWEKECPVK 534
Query: 489 PQ--PEDQPGSVILAKEPVLQANFARAVASLSKAQAKKVARFLPNPERHWGPKLRA 538
+ E P IL+ EP LQANF + ++ + + RF NPE +WGP+ RA
Sbjct: 535 RERLSETSPAFRILSVEPRLQANFTIREDANPSSRQRGLKRFQANPEANWGPRPRA 565
BLAST of MS003287 vs. ExPASy Swiss-Prot
Match:
Q9VK89 (Probable tRNA (guanine(26)-N(2))-dimethyltransferase OS=Drosophila melanogaster OX=7227 GN=CG6388 PE=2 SV=1)
HSP 1 Score: 375.6 bits (963), Expect = 1.0e-102
Identity = 219/540 (40.56%), Postives = 306/540 (56.67%), Query Frame = 0
Query: 10 IIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRAFISKRKQEHEAKLSRRTKAGQKIS 69
+I+E AEI+ + VF+N Q NRD+SIA L + + +E K ++ + K
Sbjct: 19 VIRERNAEIV--SGGNVFYNPVQEFNRDLSIAALNVYRQRLTKERSEKALKKQRKKVKEQ 78
Query: 70 ENNVSGPVVEETPNESATNDEKSNTDRDAHEETPDECGVPKLSEEQKEIIEEKEQRDPKP 129
E+ + PV E+ P + E R
Sbjct: 79 EDEKTTPVPEDPP------------------------------------VYEAGTRYEDG 138
Query: 130 LRVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEACRRNIKFNGSVACAKVESHLT 189
LR+LEAL+A+GLR++RYA+E+ GV Q+VA D + +V + NI+ N +
Sbjct: 139 LRILEALAATGLRSIRYAQEIAGVRQIVANDLSRQAVASINTNIRHNKVEELIEPSHSDA 198
Query: 190 DARVYMLTHP-KEFDMVDLDPYGSPSVFLDSAVQSVVDGGMLMCTATDMAVLCGGNGEVC 249
+Y+ T P K FD VDLDPYG P+ FLD A+Q +VDGG+L+ TATDMAVL G E C
Sbjct: 199 MTLMYLSTQPEKRFDAVDLDPYGCPNRFLDGAMQCLVDGGLLLVTATDMAVLAGNAPEAC 258
Query: 250 YSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVFVRIYTSAS 309
Y KYGS PLR K CHEMALRILL CIESHANRY +YI P+LS+ DFY+R+FVR+Y +
Sbjct: 259 YVKYGSVPLRMKCCHEMALRILLHCIESHANRYGKYIEPLLSISADFYIRIFVRVYVGQA 318
Query: 310 AMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNN-------SIRYLPGFGPSVPQECSDC 369
K + K S++YQCTGC++F LQ +G ++K N +++ GP+V +C C
Sbjct: 319 QCKLSMSKQSWIYQCTGCETFTLQPLG--ITKPNPTAGNPQQLKFGIPTGPAVNSQCEHC 378
Query: 370 GKKFNMGGPIWSAPIHDQEWVTSMLADV-KSMKDSYPAYDRISAVLTTISEELMDVPLFL 429
G + ++GGPIWSAPIH+ E+V +L V ++ S RI VL+ + EEL DVPL+
Sbjct: 379 GHRHHLGGPIWSAPIHNPEFVQDLLTAVQETTLQSLGTQRRIVGVLSMVQEELQDVPLYY 438
Query: 430 SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 489
+ LC LK + FRSA+++AGYR+S +H + LK++AP V+WDI+R W K H
Sbjct: 439 TPDKLCCVLKLEIVPMLKFRSAILHAGYRVSYSHASKNSLKTNAPPAVLWDILRSWSKRH 498
Query: 490 PVKPQP--EDQPGSVILAKEPVLQANFARAVASLS-KAQAKKVARFLPNPERHWGPKLRA 538
PV P+ P + IL+KE F + K++ ++RF NP HWGP RA
Sbjct: 499 PVNPERMIPGSPLAAILSKECTAVYEFDELHPEANPKSRKSALSRFQENPTPHWGPGTRA 518
BLAST of MS003287 vs. ExPASy TrEMBL
Match:
A0A6J1BX10 (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Momordica charantia OX=3673 GN=LOC111006460 PE=3 SV=1)
HSP 1 Score: 1152.1 bits (2979), Expect = 0.0e+00
Identity = 579/581 (99.66%), Postives = 580/581 (99.83%), Query Frame = 0
Query: 1 MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRAFISKRKQEHEAKLSR 60
MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRAFISKRKQEHEAKLSR
Sbjct: 1 MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRAFISKRKQEHEAKLSR 60
Query: 61 RTKAGQKISENNVSGPVVEETPNESATNDEKSNTDRDAHEETPDECGVPKLSEEQKEIIE 120
RTKAGQKISENNVSGPVVEETPNESATNDEKSNTDRDAHEETPDECGVPKLSEEQKEIIE
Sbjct: 61 RTKAGQKISENNVSGPVVEETPNESATNDEKSNTDRDAHEETPDECGVPKLSEEQKEIIE 120
Query: 121 EKEQRDPKPLRVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEACRRNIKFNGSVA 180
EKEQRDPKPLRVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEACRRNIKFNGSVA
Sbjct: 121 EKEQRDPKPLRVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEACRRNIKFNGSVA 180
Query: 181 CAKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSVFLDSAVQSVVDGGMLMCTATDMAVL 240
CAKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSVFLDSAVQSVVDGGMLMCTATDMAVL
Sbjct: 181 CAKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSVFLDSAVQSVVDGGMLMCTATDMAVL 240
Query: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300
CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF
Sbjct: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300
Query: 301 VRIYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSIRYLPGFGPSVPQECSD 360
VRIYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSIRYLPGFGPSVPQECSD
Sbjct: 301 VRIYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSIRYLPGFGPSVPQECSD 360
Query: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSMKDSYPAYDRISAVLTTISEELMDVPLFL 420
CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSMKD+YPAYDRISAVLTTISEELMDVPLFL
Sbjct: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSMKDTYPAYDRISAVLTTISEELMDVPLFL 420
Query: 421 SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480
SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH
Sbjct: 421 SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480
Query: 481 PVKPQPEDQPGSVILAKEPVLQANFARAVASLSKAQAKKVARFLPNPERHWGPKLRAGRQ 540
PVKPQPEDQPGSVILAKEPVLQANFARAVASLSKAQAKKVARFLPNPERHWGPKLRAGRQ
Sbjct: 481 PVKPQPEDQPGSVILAKEPVLQANFARAVASLSKAQAKKVARFLPNPERHWGPKLRAGRQ 540
Query: 541 ITSKHISLLGAEAVNGALSHTDSEEPNAKRPNVEEVALSHT 582
I SKHISLLGAEAVNGALSHTDSEEPNAKRPNVEEVALSHT
Sbjct: 541 IASKHISLLGAEAVNGALSHTDSEEPNAKRPNVEEVALSHT 581
BLAST of MS003287 vs. ExPASy TrEMBL
Match:
A0A0A0KVU4 (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucumis sativus OX=3659 GN=Csa_5G641600 PE=3 SV=1)
HSP 1 Score: 1073.2 bits (2774), Expect = 4.3e-310
Identity = 537/575 (93.39%), Postives = 555/575 (96.52%), Query Frame = 0
Query: 1 MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRAFISKRKQEHEAKLSR 60
MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLR+FI+KRKQEHEAKLS+
Sbjct: 1 MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRSFIAKRKQEHEAKLSK 60
Query: 61 RTKAGQKISENNVSGPVVEETPNESATNDEKSNTDRDAHEETPDECGVPKLSEEQKEIIE 120
R+K+GQK+SENN S EE NESATNDEK +TD DAHE+ +ECG P LSEEQKEI+E
Sbjct: 61 RSKSGQKLSENNGSETATEEACNESATNDEKCDTDCDAHEKPSNECGSPMLSEEQKEIVE 120
Query: 121 EKEQRDPKPLRVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEACRRNIKFNGSVA 180
KEQR PKP+RVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEACRRNIKFNGSVA
Sbjct: 121 GKEQRGPKPIRVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEACRRNIKFNGSVA 180
Query: 181 CAKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSVFLDSAVQSVVDGGMLMCTATDMAVL 240
C+KVESHLTDARVYMLTHPKEFDMVDLDPYGSPS+FLDSAVQSVVDGGMLMCTATDMAVL
Sbjct: 181 CSKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL 240
Query: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300
CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF
Sbjct: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300
Query: 301 VRIYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSIRYLPGFGPSVPQECSD 360
VR+YTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNS+RYLPGFGPSV QECSD
Sbjct: 301 VRVYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSVRYLPGFGPSVAQECSD 360
Query: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSMKDSYPAYDRISAVLTTISEELMDVPLFL 420
CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSMKDSYPAYDRISAVLTTISEELMDVPLFL
Sbjct: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSMKDSYPAYDRISAVLTTISEELMDVPLFL 420
Query: 421 SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480
SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH
Sbjct: 421 SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480
Query: 481 PVKPQPEDQPGSVILAKEPVLQANFARAVASLSKAQAKKVARFLPNPERHWGPKLRAGRQ 540
PVK QPEDQ GS+ILAKEPVL+ANFARAVASLSKAQAKK+ARFLPNPERHWGPKLRAGRQ
Sbjct: 481 PVKTQPEDQAGSIILAKEPVLEANFARAVASLSKAQAKKIARFLPNPERHWGPKLRAGRQ 540
Query: 541 ITSKHISLLGAEAVNGALSHTDSEEPNAKRPNVEE 576
ITSKHISLLGAEAVNGALSHTDSEEPNAKR VEE
Sbjct: 541 ITSKHISLLGAEAVNGALSHTDSEEPNAKRSKVEE 575
BLAST of MS003287 vs. ExPASy TrEMBL
Match:
A0A1S3CA23 (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucumis melo OX=3656 GN=LOC103498546 PE=3 SV=1)
HSP 1 Score: 1070.1 bits (2766), Expect = 3.2e-309
Identity = 535/575 (93.04%), Postives = 554/575 (96.35%), Query Frame = 0
Query: 1 MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRAFISKRKQEHEAKLSR 60
MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLR+FI+KRKQEHEAKLS+
Sbjct: 1 MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRSFIAKRKQEHEAKLSK 60
Query: 61 RTKAGQKISENNVSGPVVEETPNESATNDEKSNTDRDAHEETPDECGVPKLSEEQKEIIE 120
R+K+GQK+SENN S EE N SATN+EK +TD DAHE+ +ECG P LSEEQKEI+E
Sbjct: 61 RSKSGQKLSENNGSETATEEAFNGSATNEEKCHTDCDAHEKPSNECGSPMLSEEQKEIVE 120
Query: 121 EKEQRDPKPLRVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEACRRNIKFNGSVA 180
KEQR PKP+RVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEAC+RNIKFNGSVA
Sbjct: 121 GKEQRGPKPIRVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEACQRNIKFNGSVA 180
Query: 181 CAKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSVFLDSAVQSVVDGGMLMCTATDMAVL 240
C KVESHLTDARVYMLTHPKEFDMVDLDPYGSPS+FLDSAVQSVVDGGMLMCTATDMAVL
Sbjct: 181 CTKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL 240
Query: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300
CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF
Sbjct: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300
Query: 301 VRIYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSIRYLPGFGPSVPQECSD 360
VR+YTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNS+RYLPGFGPSV QECSD
Sbjct: 301 VRVYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSVRYLPGFGPSVAQECSD 360
Query: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSMKDSYPAYDRISAVLTTISEELMDVPLFL 420
CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSMKDSYPAYDRISAVLTTISEEL DVPLFL
Sbjct: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSMKDSYPAYDRISAVLTTISEELNDVPLFL 420
Query: 421 SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480
SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH
Sbjct: 421 SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480
Query: 481 PVKPQPEDQPGSVILAKEPVLQANFARAVASLSKAQAKKVARFLPNPERHWGPKLRAGRQ 540
PVK QPEDQPGS+ILAKEPVL+ANFARAVASLSKAQAKK+ARFLPNPERHWGPKLRAGRQ
Sbjct: 481 PVKAQPEDQPGSIILAKEPVLEANFARAVASLSKAQAKKIARFLPNPERHWGPKLRAGRQ 540
Query: 541 ITSKHISLLGAEAVNGALSHTDSEEPNAKRPNVEE 576
ITSKHISLLGAEAVNGALSHTDSEEPNAKRP VEE
Sbjct: 541 ITSKHISLLGAEAVNGALSHTDSEEPNAKRPKVEE 575
BLAST of MS003287 vs. ExPASy TrEMBL
Match:
A0A5A7T7H3 (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold169G002310 PE=3 SV=1)
HSP 1 Score: 1067.8 bits (2760), Expect = 1.6e-308
Identity = 534/575 (92.87%), Postives = 552/575 (96.00%), Query Frame = 0
Query: 1 MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRAFISKRKQEHEAKLSR 60
M TDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLR+FI+KRKQEHEAKLS+
Sbjct: 1 MTTDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRSFIAKRKQEHEAKLSK 60
Query: 61 RTKAGQKISENNVSGPVVEETPNESATNDEKSNTDRDAHEETPDECGVPKLSEEQKEIIE 120
R+K+GQK+SENN S EE N SATN+EK +TD DAHE+ ECG P LSEEQKEI+E
Sbjct: 61 RSKSGQKLSENNGSETATEEAFNGSATNEEKCHTDCDAHEKPSTECGSPMLSEEQKEIVE 120
Query: 121 EKEQRDPKPLRVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEACRRNIKFNGSVA 180
KEQR PKP+RVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEAC+RNIKFNGSVA
Sbjct: 121 GKEQRGPKPIRVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEACQRNIKFNGSVA 180
Query: 181 CAKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSVFLDSAVQSVVDGGMLMCTATDMAVL 240
C KVESHLTDARVYMLTHPKEFDMVDLDPYGSPS+FLDSAVQSVVDGGMLMCTATDMAVL
Sbjct: 181 CTKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL 240
Query: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300
CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF
Sbjct: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300
Query: 301 VRIYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSIRYLPGFGPSVPQECSD 360
VR+YTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNS+RYLPGFGPSV QECSD
Sbjct: 301 VRVYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSVRYLPGFGPSVAQECSD 360
Query: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSMKDSYPAYDRISAVLTTISEELMDVPLFL 420
CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSMKDSYPAYDRISAVLTTISEEL DVPLFL
Sbjct: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSMKDSYPAYDRISAVLTTISEELNDVPLFL 420
Query: 421 SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480
SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH
Sbjct: 421 SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480
Query: 481 PVKPQPEDQPGSVILAKEPVLQANFARAVASLSKAQAKKVARFLPNPERHWGPKLRAGRQ 540
PVK QPEDQPGS+ILAKEPVL+ANFARAVASLSKAQAKK+ARFLPNPERHWGPKLRAGRQ
Sbjct: 481 PVKAQPEDQPGSIILAKEPVLEANFARAVASLSKAQAKKIARFLPNPERHWGPKLRAGRQ 540
Query: 541 ITSKHISLLGAEAVNGALSHTDSEEPNAKRPNVEE 576
ITSKHISLLGAEAVNGALSHTDSEEPNAKRP VEE
Sbjct: 541 ITSKHISLLGAEAVNGALSHTDSEEPNAKRPKVEE 575
BLAST of MS003287 vs. ExPASy TrEMBL
Match:
A0A6J1GSN6 (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111456770 PE=3 SV=1)
HSP 1 Score: 1064.7 bits (2752), Expect = 1.4e-307
Identity = 531/575 (92.35%), Postives = 551/575 (95.83%), Query Frame = 0
Query: 1 MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRAFISKRKQEHEAKLSR 60
MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRD+SIAVLRAFISKRKQEHE K+SR
Sbjct: 1 MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDLSIAVLRAFISKRKQEHETKISR 60
Query: 61 RTKAGQKISENNVSGPVVEETPNESATNDEKSNTDRDAHEETPDECGVPKLSEEQKEIIE 120
R K+ QKISENN S V+EE NESATNDEK NTD DAHE+ +ECG P LSEEQKE +
Sbjct: 61 RAKSAQKISENNGSEIVMEEASNESATNDEKCNTDCDAHEKLSNECGSPVLSEEQKENVA 120
Query: 121 EKEQRDPKPLRVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEACRRNIKFNGSVA 180
KE+RD KP+RVLEALSASGLRALRYAREVEG+GQVVALDNDKASVEACRRNIKFNGS+A
Sbjct: 121 GKERRDAKPIRVLEALSASGLRALRYAREVEGIGQVVALDNDKASVEACRRNIKFNGSIA 180
Query: 181 CAKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSVFLDSAVQSVVDGGMLMCTATDMAVL 240
CAKVESHLTDARVYMLTHP EFDMVDLDPYGSPS+FLDSAVQSVVDGGMLMCTATDMAVL
Sbjct: 181 CAKVESHLTDARVYMLTHPNEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL 240
Query: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300
CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF
Sbjct: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300
Query: 301 VRIYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSIRYLPGFGPSVPQECSD 360
VR+YTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNS+RYLPGFGPSVPQECSD
Sbjct: 301 VRVYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSVRYLPGFGPSVPQECSD 360
Query: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSMKDSYPAYDRISAVLTTISEELMDVPLFL 420
CGKKFNMGGPIWSAPIHDQEWV+SMLADVKSMKD+YPAYDRISAVLTTISEELMDVPLFL
Sbjct: 361 CGKKFNMGGPIWSAPIHDQEWVSSMLADVKSMKDTYPAYDRISAVLTTISEELMDVPLFL 420
Query: 421 SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480
SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPM VIWDIMRCWVKNH
Sbjct: 421 SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMSVIWDIMRCWVKNH 480
Query: 481 PVKPQPEDQPGSVILAKEPVLQANFARAVASLSKAQAKKVARFLPNPERHWGPKLRAGRQ 540
PVK QPEDQPGS+ILAKEPVL+ANFARA+ASLSKAQAKKVARFLPNPERHWGPKLRAGRQ
Sbjct: 481 PVKAQPEDQPGSIILAKEPVLEANFARAIASLSKAQAKKVARFLPNPERHWGPKLRAGRQ 540
Query: 541 ITSKHISLLGAEAVNGALSHTDSEEPNAKRPNVEE 576
ITSKHISLLGA+AVNGALSH DSEEPNAKR VEE
Sbjct: 541 ITSKHISLLGADAVNGALSHADSEEPNAKRSKVEE 575
BLAST of MS003287 vs. TAIR 10
Match:
AT3G02320.1 (N2,N2-dimethylguanosine tRNA methyltransferase )
HSP 1 Score: 871.7 bits (2251), Expect = 3.3e-253
Identity = 431/564 (76.42%), Postives = 484/564 (85.82%), Query Frame = 0
Query: 1 MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRAFISKRKQEHEAKLSR 60
M TDL+++T+IKEGEAEILMH KN+VFFNK QVNNRD+SIAVLR F+SKRKQEHEAK S+
Sbjct: 1 METDLNDYTVIKEGEAEILMHKKNQVFFNKAQVNNRDMSIAVLREFLSKRKQEHEAKSSK 60
Query: 61 RTKAGQKISENNVSGPVVEETPNESATNDEKSNTDRDAHEETPDECGVPKLSEEQKEIIE 120
RT+ K+ E + S EETP+E+ N + E E G +S++ + E
Sbjct: 61 RTRPASKVIEKDASEASKEETPSENGMN--------NGDHEVASEDGPSSVSKDPAKTTE 120
Query: 121 EKEQRDPKPLRVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEACRRNIKFNGSVA 180
R+PKP +VLEALSASGLRALRYARE+EG+GQVVALDND ASVEAC+RNIKFNGSVA
Sbjct: 121 RFAPREPKPPKVLEALSASGLRALRYAREIEGIGQVVALDNDLASVEACQRNIKFNGSVA 180
Query: 181 CAKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSVFLDSAVQSVVDGGMLMCTATDMAVL 240
+KVESH TDARV+MLTHPKEFD+VDLDPYGSPS+FLDSA+QSV DGG+LMCTATDMAVL
Sbjct: 181 ISKVESHHTDARVHMLTHPKEFDVVDLDPYGSPSIFLDSAIQSVTDGGLLMCTATDMAVL 240
Query: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300
CGGNGEVCYSKYGSYPLR KYCHEMALRILLA IESHANRYKRYIVPVLSVQMDFYVRVF
Sbjct: 241 CGGNGEVCYSKYGSYPLRAKYCHEMALRILLASIESHANRYKRYIVPVLSVQMDFYVRVF 300
Query: 301 VRIYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSIRYLPGFGPSVPQECSD 360
VR+YTSASAMKNTPLKLSYVYQC GCDSFHLQ +GRS+ KNNS+RYLP GP V Q+C+
Sbjct: 301 VRVYTSASAMKNTPLKLSYVYQCIGCDSFHLQPVGRSLPKNNSVRYLPAIGPVVKQDCNH 360
Query: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSMKDSYPAYDRISAVLTTISEELMDVPLFL 420
CGKK+NMGGPIWSAP+HD EWVTS+L VKSMKD YPAYDRISAVLTT+SEEL+DVPLFL
Sbjct: 361 CGKKYNMGGPIWSAPMHDPEWVTSILNSVKSMKDRYPAYDRISAVLTTVSEELLDVPLFL 420
Query: 421 SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480
SLHNLCATLKC SPSA FRSAVINA YRISGTHVNPLG+K+DAPM+VIWDIMRCWVKNH
Sbjct: 421 SLHNLCATLKCISPSAAMFRSAVINANYRISGTHVNPLGMKTDAPMEVIWDIMRCWVKNH 480
Query: 481 PVKPQPEDQPGSVILAKEPVLQANFARAVASLSKAQAKKVARFLPNPERHWGPKLRAGRQ 540
P+K Q +QPGSVIL+KEP + +F+R + SLSKAQAKKVARFLPNPE+HWGPKLRAGRQ
Sbjct: 481 PIKAQSPEQPGSVILSKEPSHEVDFSRHIGSLSKAQAKKVARFLPNPEKHWGPKLRAGRQ 540
Query: 541 ITSKHISLLGAEAVNGALSHTDSE 565
ITSKH+SL+G EAVNG LS E
Sbjct: 541 ITSKHVSLIGHEAVNGHLSQHHEE 556
BLAST of MS003287 vs. TAIR 10
Match:
AT5G15810.1 (N2,N2-dimethylguanosine tRNA methyltransferase )
HSP 1 Score: 860.5 bits (2222), Expect = 7.6e-250
Identity = 432/575 (75.13%), Postives = 493/575 (85.74%), Query Frame = 0
Query: 1 MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRAFISKRKQEHEAKLSR 60
M TDL+++T+IKEGEAE+LMH KN+VFFNK QVNNRD+SIAVLRAFI KRKQEHEA LS+
Sbjct: 99 METDLNDYTVIKEGEAEVLMHKKNQVFFNKAQVNNRDMSIAVLRAFIIKRKQEHEAMLSK 158
Query: 61 RTKAGQKISENNVSGPVVEETPNESATNDEKSNTDRDAHEETPDECGVPKLSEEQKEIIE 120
R ++ K+ E +VS EETP E+ ++ K+N + HE T + PK E +
Sbjct: 159 RARSSGKVVEKDVSETSKEETPTENGDDNGKTNGE---HEVTTQD--GPK---EAAKTAY 218
Query: 121 EKEQRDPKPLRVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEACRRNIKFNGSVA 180
E +R+ KP RVLEALSASGLRALRYAREVEG+GQVVALDND ASVEAC+RNIKFNG ++
Sbjct: 219 ESARRELKPPRVLEALSASGLRALRYAREVEGIGQVVALDNDPASVEACQRNIKFNGLMS 278
Query: 181 CAKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSVFLDSAVQSVVDGGMLMCTATDMAVL 240
+KVESHLTDARV+ML+HPK+FD+VDLDPYG+PS+FLDSAVQSV DGG+LMCTATDMAVL
Sbjct: 279 TSKVESHLTDARVHMLSHPKDFDVVDLDPYGAPSIFLDSAVQSVADGGLLMCTATDMAVL 338
Query: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300
CG NGEVCYSKYGSYPL+GKYCHEMALRILLA IESHANRYKRYIVPVLSVQMDFYVRVF
Sbjct: 339 CGANGEVCYSKYGSYPLKGKYCHEMALRILLASIESHANRYKRYIVPVLSVQMDFYVRVF 398
Query: 301 VRIYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSIRYLPGFGPSVPQECSD 360
VR+YTSASAMKNTPLKLSYVYQC GCDSFHLQ +GRS+ KNNS+RYLPG GP VPQ+C+
Sbjct: 399 VRVYTSASAMKNTPLKLSYVYQCIGCDSFHLQSVGRSLPKNNSVRYLPGVGPVVPQDCTH 458
Query: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSMKDSYPAYDRISAVLTTISEELMDVPLFL 420
CGKK+NMGGPIWSAPIHDQEWV S+L VKSMKD YPAYDRI AVLTTISEEL DVPLFL
Sbjct: 459 CGKKYNMGGPIWSAPIHDQEWVNSILNGVKSMKDRYPAYDRICAVLTTISEELPDVPLFL 518
Query: 421 SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480
SLH+L ATLKCTSPSA FRSAVINA YR+SG+HVNPLG+K+DAPM++IWDIMRCWVKNH
Sbjct: 519 SLHSLSATLKCTSPSAALFRSAVINAKYRVSGSHVNPLGIKTDAPMEIIWDIMRCWVKNH 578
Query: 481 PVKPQPEDQPGSVILAKEPVLQANFARAVASLSKAQAKKVARFLPNPERHWGPKLRAGRQ 540
P+KPQ + PGSVIL+KEP QA+F+R V SLSKAQAKKVARFLPNPE+HWGPK+RAGR
Sbjct: 579 PIKPQSPEHPGSVILSKEPSHQADFSRHVGSLSKAQAKKVARFLPNPEKHWGPKIRAGRT 638
Query: 541 ITSKHISLLGAEAVNGALSHTDSEEPNAKRPNVEE 576
ITSKH+SLLG EAVNG L++ E + + EE
Sbjct: 639 ITSKHVSLLGHEAVNGHLNNNHKEAGDEEEEEEEE 665
BLAST of MS003287 vs. TAIR 10
Match:
AT3G56330.1 (N2,N2-dimethylguanosine tRNA methyltransferase )
HSP 1 Score: 95.9 bits (237), Expect = 1.1e-19
Identity = 91/360 (25.28%), Postives = 152/360 (42.22%), Query Frame = 0
Query: 124 QRDPKPLRVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEACRRNIKFNGSVACAK 183
+R LRVL+A+ G+R+LRY E E V+A D + + RR I N +K
Sbjct: 84 KRSNGSLRVLDAMCGCGIRSLRYLVEAE-ADFVMANDANDDN----RRVITDN----LSK 143
Query: 184 VESHLTDARVYMLTH-------------PKEFDMVDLDPYGSPSVFLDSAVQSVVDGGML 243
VE D R +++TH FDM+D+D +GS S FL A ++ GG+L
Sbjct: 144 VERGTGDERRWVVTHMLANKAMIERYMVADFFDMIDIDSFGSDSSFLRDAFNALRLGGLL 203
Query: 244 MCTATDMAVLCGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLS 303
T+TD G + YG++ + +E+ LR+L+ A ++ P+ S
Sbjct: 204 YLTSTDGYSSGGHRPYNSLAAYGAFIRPMPFGNEIGLRMLIGGAVREAALLGYHVTPLFS 263
Query: 304 VQMDFYVRVFVRIYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSIRYLPGF 363
++ VF + + +V C C H ++R+
Sbjct: 264 -YYSYHGPVFRVMLRVHRGKLHEDRNYGFVTHCNLCGHSH------------TLRFDELG 323
Query: 364 GPSVPQECSDCGKKFNMGGPIWSAPIHDQEWVTSMLADVKS----MKDSYPAYDRISAVL 423
P + + GP+W P+HD +VT ML K + + D++ +++
Sbjct: 324 LMGCPCSDTKASSSLVVSGPMWLGPLHDASYVTEMLELAKEWGWVSEGTGMDLDKLLSIM 383
Query: 424 TTISEELMDVPLFLSLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPM 467
S+ + P + + + + K SP SA++ GY S +H+ P LK+D PM
Sbjct: 384 IEESDPRLP-PGYTKMDEMASRAKMNSPPLKKMMSALVKEGYAASRSHIIPNALKTDCPM 420
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
XP_022134106.1 | 0.0e+00 | 99.66 | probable tRNA (guanine(26)-N(2))-dimethyltransferase 2 [Momordica charantia] | [more] |
XP_038890035.1 | 0.0e+00 | 93.91 | probable tRNA (guanine(26)-N(2))-dimethyltransferase 2 [Benincasa hispida] | [more] |
XP_011656015.1 | 8.9e-310 | 93.39 | probable tRNA (guanine(26)-N(2))-dimethyltransferase 2 [Cucumis sativus] >KGN525... | [more] |
XP_008459398.1 | 6.7e-309 | 93.04 | PREDICTED: probable tRNA (guanine(26)-N(2))-dimethyltransferase 2 [Cucumis melo] | [more] |
KAA0039424.1 | 3.4e-308 | 92.87 | putative tRNA (guanine(26)-N(2))-dimethyltransferase 2 [Cucumis melo var. makuwa... | [more] |
Match Name | E-value | Identity | Description | |
Q9SRU7 | 4.7e-252 | 76.42 | Probable tRNA (guanine(26)-N(2))-dimethyltransferase 2 OS=Arabidopsis thaliana O... | [more] |
Q9LFU5 | 1.1e-248 | 75.13 | Probable tRNA (guanine(26)-N(2))-dimethyltransferase 1 OS=Arabidopsis thaliana O... | [more] |
Q3TX08 | 1.6e-106 | 41.65 | tRNA (guanine(26)-N(2))-dimethyltransferase OS=Mus musculus OX=10090 GN=Trmt1 PE... | [more] |
Q9NXH9 | 5.6e-104 | 40.11 | tRNA (guanine(26)-N(2))-dimethyltransferase OS=Homo sapiens OX=9606 GN=TRMT1 PE=... | [more] |
Q9VK89 | 1.0e-102 | 40.56 | Probable tRNA (guanine(26)-N(2))-dimethyltransferase OS=Drosophila melanogaster ... | [more] |
Match Name | E-value | Identity | Description | |
A0A6J1BX10 | 0.0e+00 | 99.66 | tRNA (guanine(26)-N(2))-dimethyltransferase OS=Momordica charantia OX=3673 GN=LO... | [more] |
A0A0A0KVU4 | 4.3e-310 | 93.39 | tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucumis sativus OX=3659 GN=Csa_5G... | [more] |
A0A1S3CA23 | 3.2e-309 | 93.04 | tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucumis melo OX=3656 GN=LOC103498... | [more] |
A0A5A7T7H3 | 1.6e-308 | 92.87 | tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucumis melo var. makuwa OX=11946... | [more] |
A0A6J1GSN6 | 1.4e-307 | 92.35 | tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucurbita moschata OX=3662 GN=LOC... | [more] |
Match Name | E-value | Identity | Description | |
AT3G02320.1 | 3.3e-253 | 76.42 | N2,N2-dimethylguanosine tRNA methyltransferase | [more] |
AT5G15810.1 | 7.6e-250 | 75.13 | N2,N2-dimethylguanosine tRNA methyltransferase | [more] |
AT3G56330.1 | 1.1e-19 | 25.28 | N2,N2-dimethylguanosine tRNA methyltransferase | [more] |