MS003178 (gene) Bitter gourd (TR) v1

Overview
NameMS003178
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
Descriptionmetalloendoproteinase 2-MMP-like
Locationscaffold234: 921367 .. 922083 (+)
RNA-Seq ExpressionMS003178
SyntenyMS003178
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCTCCAAAGCTCTCCAAATCTTTGCTTTAATCTTATTTCTTCTTCTTTCCTTCTTCCCTCATCCAAATATGTCGTCTCATGTACTGATTTTGAAACATAATGCCATTGCCCACGACATGAACTCATCTTCCTCATTGTCTTTTAACAATCTCCGAGGATGTCGTTTGGGTGATACAGCACAAGGCATTCATCGCCTAAAAAAGTACCTTCGACGTTTCGGTTACATTACCAATGTGGAGAAGAATTCCATGGCCATGGATGACGACACCTTCGACCATGCCTTGGAGTCCGCCGTTAAAACTTACCAGAAAAACTACAATATTGATGCCTCTGGAATTTTGGACGCCAAGACCATAACCCAACTGGGTATGCCTCGATGTGGGGTTCAAGATTTCATCAATGGCACCACTTGGATGAGATCATCAATGGACCACAAAAATAGAGATGGCCGTTTCCACACAGTTTCTCACTATTCTTTCTTCGGGGGGAAAGCAAAATGGCCAGCTTCCAAGTTCCACTTGAGATATGTATTTCTTCCGGACTACCCATCTGAAGCAATTGAACCCGTGGATCAAGCTTTTTCCAAATGGGCTTCCAAAACCCACTTCACATTTCCAAAGATTTCAAACTATAAAAAATCCGATATCAAAATAAGCTTCGAAGTTGAAGAGCATGGAGATTTTGCCTCGTTGGATGGTGCGGGAGGAATTCTG

mRNA sequence

ATGGCCTCCAAAGCTCTCCAAATCTTTGCTTTAATCTTATTTCTTCTTCTTTCCTTCTTCCCTCATCCAAATATGTCGTCTCATGTACTGATTTTGAAACATAATGCCATTGCCCACGACATGAACTCATCTTCCTCATTGTCTTTTAACAATCTCCGAGGATGTCGTTTGGGTGATACAGCACAAGGCATTCATCGCCTAAAAAAGTACCTTCGACGTTTCGGTTACATTACCAATGTGGAGAAGAATTCCATGGCCATGGATGACGACACCTTCGACCATGCCTTGGAGTCCGCCGTTAAAACTTACCAGAAAAACTACAATATTGATGCCTCTGGAATTTTGGACGCCAAGACCATAACCCAACTGGGTATGCCTCGATGTGGGGTTCAAGATTTCATCAATGGCACCACTTGGATGAGATCATCAATGGACCACAAAAATAGAGATGGCCGTTTCCACACAGTTTCTCACTATTCTTTCTTCGGGGGGAAAGCAAAATGGCCAGCTTCCAAGTTCCACTTGAGATATGTATTTCTTCCGGACTACCCATCTGAAGCAATTGAACCCGTGGATCAAGCTTTTTCCAAATGGGCTTCCAAAACCCACTTCACATTTCCAAAGATTTCAAACTATAAAAAATCCGATATCAAAATAAGCTTCGAAGTTGAAGAGCATGGAGATTTTGCCTCGTTGGATGGTGCGGGAGGAATTCTG

Coding sequence (CDS)

ATGGCCTCCAAAGCTCTCCAAATCTTTGCTTTAATCTTATTTCTTCTTCTTTCCTTCTTCCCTCATCCAAATATGTCGTCTCATGTACTGATTTTGAAACATAATGCCATTGCCCACGACATGAACTCATCTTCCTCATTGTCTTTTAACAATCTCCGAGGATGTCGTTTGGGTGATACAGCACAAGGCATTCATCGCCTAAAAAAGTACCTTCGACGTTTCGGTTACATTACCAATGTGGAGAAGAATTCCATGGCCATGGATGACGACACCTTCGACCATGCCTTGGAGTCCGCCGTTAAAACTTACCAGAAAAACTACAATATTGATGCCTCTGGAATTTTGGACGCCAAGACCATAACCCAACTGGGTATGCCTCGATGTGGGGTTCAAGATTTCATCAATGGCACCACTTGGATGAGATCATCAATGGACCACAAAAATAGAGATGGCCGTTTCCACACAGTTTCTCACTATTCTTTCTTCGGGGGGAAAGCAAAATGGCCAGCTTCCAAGTTCCACTTGAGATATGTATTTCTTCCGGACTACCCATCTGAAGCAATTGAACCCGTGGATCAAGCTTTTTCCAAATGGGCTTCCAAAACCCACTTCACATTTCCAAAGATTTCAAACTATAAAAAATCCGATATCAAAATAAGCTTCGAAGTTGAAGAGCATGGAGATTTTGCCTCGTTGGATGGTGCGGGAGGAATTCTG

Protein sequence

MASKALQIFALILFLLLSFFPHPNMSSHVLILKHNAIAHDMNSSSSLSFNNLRGCRLGDTAQGIHRLKKYLRRFGYITNVEKNSMAMDDDTFDHALESAVKTYQKNYNIDASGILDAKTITQLGMPRCGVQDFINGTTWMRSSMDHKNRDGRFHTVSHYSFFGGKAKWPASKFHLRYVFLPDYPSEAIEPVDQAFSKWASKTHFTFPKISNYKKSDIKISFEVEEHGDFASLDGAGGIL
Homology
BLAST of MS003178 vs. NCBI nr
Match: XP_022134118.1 (metalloendoproteinase 3-MMP-like [Momordica charantia] >AKQ06192.1 metalloendoproteinase 1-like precursor [Momordica charantia])

HSP 1 Score: 444.9 bits (1143), Expect = 4.4e-121
Identity = 217/239 (90.79%), Postives = 224/239 (93.72%), Query Frame = 0

Query: 1   MASKALQIFALILFLLLSFFPHPNMSSHVLILKHNAIAHDMNSSSSLSFNNLRGCRLGDT 60
           M+S+A QIFA ILFLLLSFFPH NMSS VLILKHNAIAHDMNSS SLSFNNLRGCRLGDT
Sbjct: 1   MSSQAFQIFAFILFLLLSFFPHLNMSSRVLILKHNAIAHDMNSSFSLSFNNLRGCRLGDT 60

Query: 61  AQGIHRLKKYLRRFGYITNVEKNSMAMDDDTFDHALESAVKTYQKNYNIDASGILDAKTI 120
           AQGIHRLKKYLRRFGYITNVEKNSMAMDDDTFDHALESAVKTYQKNYNIDASGILDAKTI
Sbjct: 61  AQGIHRLKKYLRRFGYITNVEKNSMAMDDDTFDHALESAVKTYQKNYNIDASGILDAKTI 120

Query: 121 TQLGMPRCGVQDFINGTTWMRSSMDHKNRDGRFHTVSHYSFFGGKAKWPASKFHLRYVFL 180
           TQ+  PRCGVQDF NGTTWMRSSMDHKNRDGRFHTVSHYSFFGGK KWPASKFHL Y FL
Sbjct: 121 TQMRTPRCGVQDFFNGTTWMRSSMDHKNRDGRFHTVSHYSFFGGKPKWPASKFHLSYAFL 180

Query: 181 PDYPSEAIEPVDQAFSKWASKTHFTFPKISNYKKSDIKISFEVEEHGDFASLDGAGGIL 240
           P YPSEAIEPVD+AFSKWAS+THFTF KISNYKKSDIKISFE+E+HGDFAS DG GGIL
Sbjct: 181 PGYPSEAIEPVDRAFSKWASETHFTFSKISNYKKSDIKISFEIEDHGDFASFDGMGGIL 239

BLAST of MS003178 vs. NCBI nr
Match: XP_022134170.1 (metalloendoproteinase 2-MMP-like [Momordica charantia])

HSP 1 Score: 410.2 bits (1053), Expect = 1.2e-110
Identity = 204/241 (84.65%), Postives = 217/241 (90.04%), Query Frame = 0

Query: 1   MASKALQIFALILFLLLSFFPHPNMSSHVLILKHNAIAHDMNSSSSLSFNNLRGCRLGDT 60
           MASKALQIFA ILFLLLSFFPH NMSSHVLILK NAIAHDMNSSSSLSFNNLRGCRLGDT
Sbjct: 1   MASKALQIFAFILFLLLSFFPHLNMSSHVLILKQNAIAHDMNSSSSLSFNNLRGCRLGDT 60

Query: 61  AQGIHRLKKYLRRFGYITNVEKNSMAMDDDTFDHALESAVKTYQKNYNIDASGILDAKTI 120
           AQGIHRLKKYLRRFGYITNVEKNSMAMDDDTFDHALESAVKTYQKNYNIDASGILDAKTI
Sbjct: 61  AQGIHRLKKYLRRFGYITNVEKNSMAMDDDTFDHALESAVKTYQKNYNIDASGILDAKTI 120

Query: 121 TQLGMPRCGVQDFINGTTWMRSSMDHKNRDG--RFHTVSHYSFFGGKAKWPASKFHLRYV 180
           TQ+GMPRCGVQDFINGTTWMRSS   +N++G   FH VSH++FF G  KWPASKFHL Y 
Sbjct: 121 TQMGMPRCGVQDFINGTTWMRSSGRRRNQNGHSHFHKVSHFAFFEGNLKWPASKFHLSYA 180

Query: 181 FLPDYPSEAIEPVDQAFSKWASKTHFTFPKISNYKKSDIKISFEVEEHGDFASLDGAGGI 240
           FLP YPSEAI PV +AFSKWAS THFTF  +S++K +DIKISFE+EEHGD A+ DGAGGI
Sbjct: 181 FLPGYPSEAILPVSRAFSKWASNTHFTFSHVSDHKTADIKISFEIEEHGDDAAFDGAGGI 240

BLAST of MS003178 vs. NCBI nr
Match: XP_022962668.1 (metalloendoproteinase 2-MMP-like [Cucurbita moschata])

HSP 1 Score: 295.0 bits (754), Expect = 5.6e-76
Identity = 155/253 (61.26%), Postives = 183/253 (72.33%), Query Frame = 0

Query: 1   MASKALQIFALILFLLLSFFPH------------PNMSSHVLILKHNAIAHDMNSSSSLS 60
           MASK LQ+ A  L LL SFFPH              + S  +IL HN  +H  NSSS L 
Sbjct: 1   MASKPLQLLAFTL-LLPSFFPHFDIVSVQCHSESEAVGSSPIILNHN--SHATNSSSLLF 60

Query: 61  FNNLRGCRLGDTAQGIHRLKKYLRRFGYITNVEKNSMAMD-DDTFDHALESAVKTYQKNY 120
            NNL+GCRLGDTAQGIHR+KKYL+RFGYITNV+K S AMD DDTFD+ALESA+KTYQKN+
Sbjct: 61  LNNLQGCRLGDTAQGIHRIKKYLQRFGYITNVQKQSNAMDHDDTFDNALESALKTYQKNF 120

Query: 121 NIDASGILDAKTITQLGMPRCGVQDFINGTTWMRSSMDHKNR-DGRFHTVSHYSFFGGKA 180
           N+ ASGILDA T+ Q+G PRCGV+D INGTTWMRS  + K +    FH VSH++FF G  
Sbjct: 121 NLVASGILDANTLAQMGKPRCGVEDVINGTTWMRSGRERKPKMHAHFHLVSHFAFFEGNL 180

Query: 181 KWPASKFHLRYVFLPDYPSEAIEPVDQAFSKWASKTHFTFPKISNYKKSDIKISFEVEEH 240
           KWP SKFHL Y FLP YP EA+  V +AFSKWA  THFTF ++ ++ K+DIKISFE+ +H
Sbjct: 181 KWPPSKFHLTYAFLPGYPFEAMMAVSRAFSKWAFNTHFTFSRVFDHTKADIKISFEIGDH 240

BLAST of MS003178 vs. NCBI nr
Match: XP_022991021.1 (metalloendoproteinase 2-MMP-like [Cucurbita maxima])

HSP 1 Score: 291.2 bits (744), Expect = 8.1e-75
Identity = 152/253 (60.08%), Postives = 185/253 (73.12%), Query Frame = 0

Query: 1   MASKALQIFALILFLLLSFFPHPNMS------------SHVLILKHNAIAHDMNSSSSLS 60
           MASK LQ+ A  L LL SFFPH +++            S  +IL HN  +H  NS+S L 
Sbjct: 1   MASKPLQLLAFTL-LLPSFFPHFDIASVQCHSESKVVGSSPIILNHN--SHATNSASLLF 60

Query: 61  FNNLRGCRLGDTAQGIHRLKKYLRRFGYITNVEKNSMAMD-DDTFDHALESAVKTYQKNY 120
            NNL+GCRLGDT QGIHR+KKYL+RFGYI+NV+K+S AMD DDTFD+ LESA+KTYQKN+
Sbjct: 61  LNNLQGCRLGDTVQGIHRIKKYLQRFGYISNVQKHSNAMDHDDTFDNTLESALKTYQKNF 120

Query: 121 NIDASGILDAKTITQLGMPRCGVQDFINGTTWMRSSMDHKNR-DGRFHTVSHYSFFGGKA 180
           N+ ASGILDA T+ Q+G PRCGV+D INGTTWMRS  +   +    FH VSH++FF G  
Sbjct: 121 NLVASGILDANTLAQMGKPRCGVEDVINGTTWMRSGRERNPKMHAHFHLVSHFAFFEGNL 180

Query: 181 KWPASKFHLRYVFLPDYPSEAIEPVDQAFSKWASKTHFTFPKISNYKKSDIKISFEVEEH 240
           KWP SKFHL Y FLP YP EA+  V +AFSKWA  THFTF ++ ++ K+DIKISFE+ +H
Sbjct: 181 KWPPSKFHLTYAFLPGYPFEAMMAVSRAFSKWAFNTHFTFSRVFDHTKADIKISFEIGDH 240

BLAST of MS003178 vs. NCBI nr
Match: KAG6602462.1 (Metalloendoproteinase 2-MMP, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 285.8 bits (730), Expect = 3.4e-73
Identity = 153/253 (60.47%), Postives = 180/253 (71.15%), Query Frame = 0

Query: 1   MASKALQIFALILFLLLSFFPH------------PNMSSHVLILKHNAIAHDMNSSSSLS 60
           MASK LQ+ A  L LL S FP               + S  +IL  N  +H  NSSS L 
Sbjct: 1   MASKPLQLLAFTL-LLPSLFPRFDIVSVQCHSELEAVGSSPIILSPN--SHAPNSSSLLF 60

Query: 61  FNNLRGCRLGDTAQGIHRLKKYLRRFGYITNVEKNSMAMD-DDTFDHALESAVKTYQKNY 120
            NNL+GCRLGDTAQGIHR+KKYL+RFGYITNV+K S AMD DDTFD+ALESA+KTYQKN+
Sbjct: 61  LNNLQGCRLGDTAQGIHRIKKYLQRFGYITNVQKQSNAMDHDDTFDNALESALKTYQKNF 120

Query: 121 NIDASGILDAKTITQLGMPRCGVQDFINGTTWMRSSMDHKNR-DGRFHTVSHYSFFGGKA 180
           N+ ASGILDA T+ Q+G PRCGV+D INGTTWMRS  + K +    FH VSH++FF G  
Sbjct: 121 NLVASGILDANTLAQMGKPRCGVEDVINGTTWMRSGRERKPKMHAHFHLVSHFAFFEGNL 180

Query: 181 KWPASKFHLRYVFLPDYPSEAIEPVDQAFSKWASKTHFTFPKISNYKKSDIKISFEVEEH 240
           KWP SKFHL Y FLP YP EA+  V +AFSKWA  THFTF ++  + K+DIKISFE+ +H
Sbjct: 181 KWPPSKFHLTYAFLPGYPFEAMMAVSRAFSKWAFNTHFTFSRVFYHTKADIKISFEIGDH 240

BLAST of MS003178 vs. ExPASy Swiss-Prot
Match: Q5XF51 (Metalloendoproteinase 3-MMP OS=Arabidopsis thaliana OX=3702 GN=3MMP PE=1 SV=1)

HSP 1 Score: 142.1 bits (357), Expect = 8.0e-33
Identity = 87/238 (36.55%), Postives = 124/238 (52.10%), Query Frame = 0

Query: 11  LILFLLLSFFPHP-------NMSSHVLILKHNAIAHDMNSSSSLSFNNLRGCRLGDTAQG 70
           + +  LL F P P       N S+    L  NA  +  N     SF N  GC  G    G
Sbjct: 6   VFMVFLLFFAPSPVSAGFYTNSSAIPPQLLRNATGNPWN-----SFLNFTGCHAGKKYDG 65

Query: 71  IHRLKKYLRRFGYITNVEKNSMAMDDDTFDHALESAVKTYQKNYNIDASGILDAKTITQL 130
           ++ LK+Y + FGYIT  E N      D FD  L++AV+ YQ+N+ ++ +G+LD  T+  +
Sbjct: 66  LYMLKQYFQHFGYIT--ETNLSGNFTDDFDDILKNAVEMYQRNFQLNVTGVLDELTLKHV 125

Query: 131 GMPRCGVQDFINGTTWMRS-----SMDHKNRDGRFHTVSHYSFFGGKAKWPASKFHLRYV 190
            +PRCG  D +NGT+ M S      +    R  RFH V HYSFF G+ +WP ++  L Y 
Sbjct: 126 VIPRCGNPDVVNGTSTMHSGRKTFEVSFAGRGQRFHAVKHYSFFPGEPRWPRNRRDLTYA 185

Query: 191 FLP-DYPSEAIEPV-DQAFSKWASKTHFTFPKISNYKKSDIKISFEVEEHGDFASLDG 235
           F P +  +E ++ V  +AF++W   T  TF ++  +  SDI I F   EHGD    DG
Sbjct: 186 FDPRNALTEEVKSVFSRAFTRWEEVTPLTFTRVERFSTSDISIGFYSGEHGDGEPFDG 236

BLAST of MS003178 vs. ExPASy Swiss-Prot
Match: Q9ZUJ5 (Metalloendoproteinase 5-MMP OS=Arabidopsis thaliana OX=3702 GN=5MMP PE=1 SV=1)

HSP 1 Score: 138.7 bits (348), Expect = 8.9e-32
Identity = 76/194 (39.18%), Postives = 103/194 (53.09%), Query Frame = 0

Query: 48  SFNNLRGCRLGDTAQGIHRLKKYLRRFGYITNVEKNSMAMDDDTFDHALESAVKTYQKNY 107
           +F+ L GC +G+   G+ +LK+Y RRFGYIT     +     D FD  L+SA+ TYQKN+
Sbjct: 44  TFSKLAGCHIGENINGLSKLKQYFRRFGYITTTGNCT-----DDFDDVLQSAINTYQKNF 103

Query: 108 NIDASGILDAKTITQLGMPRCGVQDFINGTTWMRSSMDHKNRDGRFHTVSHYSFFGGKAK 167
           N+  +G LD+ T+ Q+  PRCG  D I+G + M       N      T   YSFF GK +
Sbjct: 104 NLKVTGKLDSSTLRQIVKPRCGNPDLIDGVSEM-------NGGKILRTTEKYSFFPGKPR 163

Query: 168 WPASKFHLRYVFLP--DYPSEAIEPVDQAFSKWASKTHFTFPKISNYKKSDIKISFEVEE 227
           WP  K  L Y F P  +   E      +AF++WA  T   F +  +  ++DI I F   E
Sbjct: 164 WPKRKRDLTYAFAPQNNLTDEVKRVFSRAFTRWAEVTPLNFTRSESILRADIVIGFFSGE 223

Query: 228 HGDFASLDGAGGIL 240
           HGD    DGA G L
Sbjct: 224 HGDGEPFDGAMGTL 225

BLAST of MS003178 vs. ExPASy Swiss-Prot
Match: O04529 (Metalloendoproteinase 2-MMP OS=Arabidopsis thaliana OX=3702 GN=2MMP PE=1 SV=1)

HSP 1 Score: 134.4 bits (337), Expect = 1.7e-30
Identity = 72/198 (36.36%), Postives = 106/198 (53.54%), Query Frame = 0

Query: 48  SFNNLRGCRLGDTAQGIHRLKKYLRRFGYITNVEKNSMAMDDDTFDHALESAVKTYQKNY 107
           +F+N  GC  G    G++R+KKY +RFGYI      +     D FD  L++AV+ YQ N+
Sbjct: 43  AFSNFTGCHHGQNVDGLYRIKKYFQRFGYIPETFSGNFT---DDFDDILKAAVELYQTNF 102

Query: 108 NIDASGILDAKTITQLGMPRCGVQDFINGTTWM----RSSMDHKNRDGRFHTVSHYSFFG 167
           N++ +G LDA TI  + +PRCG  D +NGT+ M    R + +        H V  Y+ F 
Sbjct: 103 NLNVTGELDALTIQHIVIPRCGNPDVVNGTSLMHGGRRKTFEVNFSRTHLHAVKRYTLFP 162

Query: 168 GKAKWPASKFHLRYVFLPDYP-SEAIEPV-DQAFSKWASKTHFTFPKISNYKKSDIKISF 227
           G+ +WP ++  L Y F P  P +E ++ V  +AF +W+  T   F    ++  SDI I F
Sbjct: 163 GEPRWPRNRRDLTYAFDPKNPLTEEVKSVFSRAFGRWSDVTALNFTLSESFSTSDITIGF 222

Query: 228 EVEEHGDFASLDGAGGIL 240
              +HGD    DG  G L
Sbjct: 223 YTGDHGDGEPFDGVLGTL 237

BLAST of MS003178 vs. ExPASy Swiss-Prot
Match: P29136 (Metalloendoproteinase 1 OS=Glycine max OX=3847 PE=1 SV=2)

HSP 1 Score: 119.8 bits (299), Expect = 4.3e-26
Identity = 67/184 (36.41%), Postives = 91/184 (49.46%), Query Frame = 0

Query: 58  GDTAQGIHRLKKYLRRFGYITNVEKNSMAMDDDTFDHALESAVKTYQKNYNIDASGILDA 117
           G   +G+  +K Y    GYI N         DD FD  L SA+KTYQKNYN++ +G  D 
Sbjct: 48  GQNYKGLSNVKNYFHHLGYIPNAPH-----FDDNFDDTLVSAIKTYQKNYNLNVTGKFDI 107

Query: 118 KTITQLGMPRCGVQDFINGTTWMRSSMDHKNRDGRFHTVSHYSFFGGKAKWPASKFHLRY 177
            T+ Q+  PRCGV D I  T          N+   F  +S Y+FF    +W A    L Y
Sbjct: 108 NTLKQIMTPRCGVPDIIINT----------NKTTSFGMISDYTFFKDMPRWQAGTTQLTY 167

Query: 178 VFLPD--YPSEAIEPVDQAFSKWASKTHFTFPKISNYKKSDIKISFEVEEHGDFASLDGA 237
            F P+          + +AFSKW    +  F + ++Y+ ++IKI F  + HGD    DG 
Sbjct: 168 AFSPEPRLDDTFKSAIARAFSKWTPVVNIAFQETTSYETANIKILFASKNHGDPYPFDGP 216

Query: 238 GGIL 240
           GGIL
Sbjct: 228 GGIL 216

BLAST of MS003178 vs. ExPASy Swiss-Prot
Match: O23507 (Metalloendoproteinase 1-MMP OS=Arabidopsis thaliana OX=3702 GN=1MMP PE=1 SV=1)

HSP 1 Score: 110.5 bits (275), Expect = 2.6e-23
Identity = 80/238 (33.61%), Postives = 116/238 (48.74%), Query Frame = 0

Query: 9   FALILFLLLSFFPHPNM-SSHVLILKHNAIAHDMNSSSSLSFNNLRGCRLGDTAQGIHRL 68
           F LILF     F   N   +     K   I H  NS+    F+ L   ++G    G+  L
Sbjct: 15  FVLILFCFPYRFGARNTPEAEQSTAKATQIIHVSNSTWH-DFSRLVDVQIGSHVSGVSEL 74

Query: 69  KKYLRRFGYITNVEKNSMAMDDDTFDHALESAVKTYQKNYNIDASGILDAKTITQLGMPR 128
           K+YL RFGY+     +   +  D FD  LESA+  YQ+N  +  +G LD  T+T + +PR
Sbjct: 75  KRYLHRFGYV----NDGSEIFSDVFDGPLESAISLYQENLGLPITGRLDTSTVTLMSLPR 134

Query: 129 CGVQDFINGTTWMRSSMDHKNRDGRFHTVSHYSFFGGKAKWPASKFHLRYVF-----LPD 188
           CGV D     T M  + D        HT +HY++F GK KW  ++  L Y       L  
Sbjct: 135 CGVSD-----THMTINNDF------LHTTAHYTYFNGKPKW--NRDTLTYAISKTHKLDY 194

Query: 189 YPSEAIEPV-DQAFSKWASKTHFTFPKISNYKKSDIKISFEVEEHGDFASLDGAGGIL 240
             SE ++ V  +AFS+W+S    +F ++ ++  +D+KI F   +HGD    DG  G L
Sbjct: 195 LTSEDVKTVFRRAFSQWSSVIPVSFEEVDDFTTADLKIGFYAGDHGDGLPFDGVLGTL 234

BLAST of MS003178 vs. ExPASy TrEMBL
Match: A0A0H4TG97 (Metalloendoproteinase 1-like OS=Momordica charantia OX=3673 GN=LOC111006468 PE=2 SV=1)

HSP 1 Score: 444.9 bits (1143), Expect = 2.1e-121
Identity = 217/239 (90.79%), Postives = 224/239 (93.72%), Query Frame = 0

Query: 1   MASKALQIFALILFLLLSFFPHPNMSSHVLILKHNAIAHDMNSSSSLSFNNLRGCRLGDT 60
           M+S+A QIFA ILFLLLSFFPH NMSS VLILKHNAIAHDMNSS SLSFNNLRGCRLGDT
Sbjct: 1   MSSQAFQIFAFILFLLLSFFPHLNMSSRVLILKHNAIAHDMNSSFSLSFNNLRGCRLGDT 60

Query: 61  AQGIHRLKKYLRRFGYITNVEKNSMAMDDDTFDHALESAVKTYQKNYNIDASGILDAKTI 120
           AQGIHRLKKYLRRFGYITNVEKNSMAMDDDTFDHALESAVKTYQKNYNIDASGILDAKTI
Sbjct: 61  AQGIHRLKKYLRRFGYITNVEKNSMAMDDDTFDHALESAVKTYQKNYNIDASGILDAKTI 120

Query: 121 TQLGMPRCGVQDFINGTTWMRSSMDHKNRDGRFHTVSHYSFFGGKAKWPASKFHLRYVFL 180
           TQ+  PRCGVQDF NGTTWMRSSMDHKNRDGRFHTVSHYSFFGGK KWPASKFHL Y FL
Sbjct: 121 TQMRTPRCGVQDFFNGTTWMRSSMDHKNRDGRFHTVSHYSFFGGKPKWPASKFHLSYAFL 180

Query: 181 PDYPSEAIEPVDQAFSKWASKTHFTFPKISNYKKSDIKISFEVEEHGDFASLDGAGGIL 240
           P YPSEAIEPVD+AFSKWAS+THFTF KISNYKKSDIKISFE+E+HGDFAS DG GGIL
Sbjct: 181 PGYPSEAIEPVDRAFSKWASETHFTFSKISNYKKSDIKISFEIEDHGDFASFDGMGGIL 239

BLAST of MS003178 vs. ExPASy TrEMBL
Match: A0A6J1BXD5 (metalloendoproteinase 2-MMP-like OS=Momordica charantia OX=3673 GN=LOC111006498 PE=3 SV=1)

HSP 1 Score: 410.2 bits (1053), Expect = 5.8e-111
Identity = 204/241 (84.65%), Postives = 217/241 (90.04%), Query Frame = 0

Query: 1   MASKALQIFALILFLLLSFFPHPNMSSHVLILKHNAIAHDMNSSSSLSFNNLRGCRLGDT 60
           MASKALQIFA ILFLLLSFFPH NMSSHVLILK NAIAHDMNSSSSLSFNNLRGCRLGDT
Sbjct: 1   MASKALQIFAFILFLLLSFFPHLNMSSHVLILKQNAIAHDMNSSSSLSFNNLRGCRLGDT 60

Query: 61  AQGIHRLKKYLRRFGYITNVEKNSMAMDDDTFDHALESAVKTYQKNYNIDASGILDAKTI 120
           AQGIHRLKKYLRRFGYITNVEKNSMAMDDDTFDHALESAVKTYQKNYNIDASGILDAKTI
Sbjct: 61  AQGIHRLKKYLRRFGYITNVEKNSMAMDDDTFDHALESAVKTYQKNYNIDASGILDAKTI 120

Query: 121 TQLGMPRCGVQDFINGTTWMRSSMDHKNRDG--RFHTVSHYSFFGGKAKWPASKFHLRYV 180
           TQ+GMPRCGVQDFINGTTWMRSS   +N++G   FH VSH++FF G  KWPASKFHL Y 
Sbjct: 121 TQMGMPRCGVQDFINGTTWMRSSGRRRNQNGHSHFHKVSHFAFFEGNLKWPASKFHLSYA 180

Query: 181 FLPDYPSEAIEPVDQAFSKWASKTHFTFPKISNYKKSDIKISFEVEEHGDFASLDGAGGI 240
           FLP YPSEAI PV +AFSKWAS THFTF  +S++K +DIKISFE+EEHGD A+ DGAGGI
Sbjct: 181 FLPGYPSEAILPVSRAFSKWASNTHFTFSHVSDHKTADIKISFEIEEHGDDAAFDGAGGI 240

BLAST of MS003178 vs. ExPASy TrEMBL
Match: A0A6J1HFG1 (metalloendoproteinase 2-MMP-like OS=Cucurbita moschata OX=3662 GN=LOC111463086 PE=3 SV=1)

HSP 1 Score: 295.0 bits (754), Expect = 2.7e-76
Identity = 155/253 (61.26%), Postives = 183/253 (72.33%), Query Frame = 0

Query: 1   MASKALQIFALILFLLLSFFPH------------PNMSSHVLILKHNAIAHDMNSSSSLS 60
           MASK LQ+ A  L LL SFFPH              + S  +IL HN  +H  NSSS L 
Sbjct: 1   MASKPLQLLAFTL-LLPSFFPHFDIVSVQCHSESEAVGSSPIILNHN--SHATNSSSLLF 60

Query: 61  FNNLRGCRLGDTAQGIHRLKKYLRRFGYITNVEKNSMAMD-DDTFDHALESAVKTYQKNY 120
            NNL+GCRLGDTAQGIHR+KKYL+RFGYITNV+K S AMD DDTFD+ALESA+KTYQKN+
Sbjct: 61  LNNLQGCRLGDTAQGIHRIKKYLQRFGYITNVQKQSNAMDHDDTFDNALESALKTYQKNF 120

Query: 121 NIDASGILDAKTITQLGMPRCGVQDFINGTTWMRSSMDHKNR-DGRFHTVSHYSFFGGKA 180
           N+ ASGILDA T+ Q+G PRCGV+D INGTTWMRS  + K +    FH VSH++FF G  
Sbjct: 121 NLVASGILDANTLAQMGKPRCGVEDVINGTTWMRSGRERKPKMHAHFHLVSHFAFFEGNL 180

Query: 181 KWPASKFHLRYVFLPDYPSEAIEPVDQAFSKWASKTHFTFPKISNYKKSDIKISFEVEEH 240
           KWP SKFHL Y FLP YP EA+  V +AFSKWA  THFTF ++ ++ K+DIKISFE+ +H
Sbjct: 181 KWPPSKFHLTYAFLPGYPFEAMMAVSRAFSKWAFNTHFTFSRVFDHTKADIKISFEIGDH 240

BLAST of MS003178 vs. ExPASy TrEMBL
Match: A0A6J1JPK1 (metalloendoproteinase 2-MMP-like OS=Cucurbita maxima OX=3661 GN=LOC111487736 PE=3 SV=1)

HSP 1 Score: 291.2 bits (744), Expect = 3.9e-75
Identity = 152/253 (60.08%), Postives = 185/253 (73.12%), Query Frame = 0

Query: 1   MASKALQIFALILFLLLSFFPHPNMS------------SHVLILKHNAIAHDMNSSSSLS 60
           MASK LQ+ A  L LL SFFPH +++            S  +IL HN  +H  NS+S L 
Sbjct: 1   MASKPLQLLAFTL-LLPSFFPHFDIASVQCHSESKVVGSSPIILNHN--SHATNSASLLF 60

Query: 61  FNNLRGCRLGDTAQGIHRLKKYLRRFGYITNVEKNSMAMD-DDTFDHALESAVKTYQKNY 120
            NNL+GCRLGDT QGIHR+KKYL+RFGYI+NV+K+S AMD DDTFD+ LESA+KTYQKN+
Sbjct: 61  LNNLQGCRLGDTVQGIHRIKKYLQRFGYISNVQKHSNAMDHDDTFDNTLESALKTYQKNF 120

Query: 121 NIDASGILDAKTITQLGMPRCGVQDFINGTTWMRSSMDHKNR-DGRFHTVSHYSFFGGKA 180
           N+ ASGILDA T+ Q+G PRCGV+D INGTTWMRS  +   +    FH VSH++FF G  
Sbjct: 121 NLVASGILDANTLAQMGKPRCGVEDVINGTTWMRSGRERNPKMHAHFHLVSHFAFFEGNL 180

Query: 181 KWPASKFHLRYVFLPDYPSEAIEPVDQAFSKWASKTHFTFPKISNYKKSDIKISFEVEEH 240
           KWP SKFHL Y FLP YP EA+  V +AFSKWA  THFTF ++ ++ K+DIKISFE+ +H
Sbjct: 181 KWPPSKFHLTYAFLPGYPFEAMMAVSRAFSKWAFNTHFTFSRVFDHTKADIKISFEIGDH 240

BLAST of MS003178 vs. ExPASy TrEMBL
Match: Q9LEL9 (Matrix metalloproteinase OS=Cucumis sativus OX=3659 GN=MMP-1 PE=2 SV=1)

HSP 1 Score: 266.5 bits (680), Expect = 1.0e-67
Identity = 143/243 (58.85%), Postives = 177/243 (72.84%), Query Frame = 0

Query: 1   MAS-KALQIFALILFLLLSFFPHPNMSSHVLILKHNAIAHDMNSSSSLSF-NNLRGCRLG 60
           MAS KALQI      L LS FP+PN SS + ILKH+  + +MNSS+SL F  NL+GC LG
Sbjct: 1   MASPKALQIIFPFTLLFLSLFPNPNTSSPI-ILKHS--SQNMNSSNSLMFLKNLQGCHLG 60

Query: 61  DTAQGIHRLKKYLRRFGYI-TNVEKNSMAMDDDTFDHALESAVKTYQKNYNIDASGILDA 120
           DT QGIH++KKYL+RFGYI TN++K+S  + DDTFDH LESA+KTYQ N+N+  SGILD+
Sbjct: 61  DTKQGIHQIKKYLQRFGYITTNIQKHSNPIFDDTFDHILESALKTYQTNHNLAPSGILDS 120

Query: 121 KTITQLGMPRCGVQDFI-NGTTWMRSSMDHKNRDGRFHTVSHYSFFGGKAKWPASKFHLR 180
            TI Q+ MPRCGVQD I N  T  R+     N    FH VSH++FF G  KWP+SK HL 
Sbjct: 121 NTIAQIAMPRCGVQDVIKNKKTKKRNQNFTNNGHTHFHKVSHFTFFEGNLKWPSSKLHLS 180

Query: 181 YVFLPDYPSEAIEPVDQAFSKWASKTHFTFPKISNYKKSDIKISFEVEEHGDFASLDGAG 240
           Y FLP+YP +AI+PV +AFSKW+  THF F  +++Y+K+DIKISFE  EHGD A  DG G
Sbjct: 181 YGFLPNYPIDAIKPVSRAFSKWSLNTHFKFSHVADYRKADIKISFERGEHGDNAPFDGVG 240

BLAST of MS003178 vs. TAIR 10
Match: AT1G24140.1 (Matrixin family protein )

HSP 1 Score: 142.1 bits (357), Expect = 5.7e-34
Identity = 87/238 (36.55%), Postives = 124/238 (52.10%), Query Frame = 0

Query: 11  LILFLLLSFFPHP-------NMSSHVLILKHNAIAHDMNSSSSLSFNNLRGCRLGDTAQG 70
           + +  LL F P P       N S+    L  NA  +  N     SF N  GC  G    G
Sbjct: 6   VFMVFLLFFAPSPVSAGFYTNSSAIPPQLLRNATGNPWN-----SFLNFTGCHAGKKYDG 65

Query: 71  IHRLKKYLRRFGYITNVEKNSMAMDDDTFDHALESAVKTYQKNYNIDASGILDAKTITQL 130
           ++ LK+Y + FGYIT  E N      D FD  L++AV+ YQ+N+ ++ +G+LD  T+  +
Sbjct: 66  LYMLKQYFQHFGYIT--ETNLSGNFTDDFDDILKNAVEMYQRNFQLNVTGVLDELTLKHV 125

Query: 131 GMPRCGVQDFINGTTWMRS-----SMDHKNRDGRFHTVSHYSFFGGKAKWPASKFHLRYV 190
            +PRCG  D +NGT+ M S      +    R  RFH V HYSFF G+ +WP ++  L Y 
Sbjct: 126 VIPRCGNPDVVNGTSTMHSGRKTFEVSFAGRGQRFHAVKHYSFFPGEPRWPRNRRDLTYA 185

Query: 191 FLP-DYPSEAIEPV-DQAFSKWASKTHFTFPKISNYKKSDIKISFEVEEHGDFASLDG 235
           F P +  +E ++ V  +AF++W   T  TF ++  +  SDI I F   EHGD    DG
Sbjct: 186 FDPRNALTEEVKSVFSRAFTRWEEVTPLTFTRVERFSTSDISIGFYSGEHGDGEPFDG 236

BLAST of MS003178 vs. TAIR 10
Match: AT1G59970.1 (Matrixin family protein )

HSP 1 Score: 138.7 bits (348), Expect = 6.3e-33
Identity = 76/194 (39.18%), Postives = 103/194 (53.09%), Query Frame = 0

Query: 48  SFNNLRGCRLGDTAQGIHRLKKYLRRFGYITNVEKNSMAMDDDTFDHALESAVKTYQKNY 107
           +F+ L GC +G+   G+ +LK+Y RRFGYIT     +     D FD  L+SA+ TYQKN+
Sbjct: 44  TFSKLAGCHIGENINGLSKLKQYFRRFGYITTTGNCT-----DDFDDVLQSAINTYQKNF 103

Query: 108 NIDASGILDAKTITQLGMPRCGVQDFINGTTWMRSSMDHKNRDGRFHTVSHYSFFGGKAK 167
           N+  +G LD+ T+ Q+  PRCG  D I+G + M       N      T   YSFF GK +
Sbjct: 104 NLKVTGKLDSSTLRQIVKPRCGNPDLIDGVSEM-------NGGKILRTTEKYSFFPGKPR 163

Query: 168 WPASKFHLRYVFLP--DYPSEAIEPVDQAFSKWASKTHFTFPKISNYKKSDIKISFEVEE 227
           WP  K  L Y F P  +   E      +AF++WA  T   F +  +  ++DI I F   E
Sbjct: 164 WPKRKRDLTYAFAPQNNLTDEVKRVFSRAFTRWAEVTPLNFTRSESILRADIVIGFFSGE 223

Query: 228 HGDFASLDGAGGIL 240
           HGD    DGA G L
Sbjct: 224 HGDGEPFDGAMGTL 225

BLAST of MS003178 vs. TAIR 10
Match: AT1G70170.1 (matrix metalloproteinase )

HSP 1 Score: 134.4 bits (337), Expect = 1.2e-31
Identity = 72/198 (36.36%), Postives = 106/198 (53.54%), Query Frame = 0

Query: 48  SFNNLRGCRLGDTAQGIHRLKKYLRRFGYITNVEKNSMAMDDDTFDHALESAVKTYQKNY 107
           +F+N  GC  G    G++R+KKY +RFGYI      +     D FD  L++AV+ YQ N+
Sbjct: 43  AFSNFTGCHHGQNVDGLYRIKKYFQRFGYIPETFSGNFT---DDFDDILKAAVELYQTNF 102

Query: 108 NIDASGILDAKTITQLGMPRCGVQDFINGTTWM----RSSMDHKNRDGRFHTVSHYSFFG 167
           N++ +G LDA TI  + +PRCG  D +NGT+ M    R + +        H V  Y+ F 
Sbjct: 103 NLNVTGELDALTIQHIVIPRCGNPDVVNGTSLMHGGRRKTFEVNFSRTHLHAVKRYTLFP 162

Query: 168 GKAKWPASKFHLRYVFLPDYP-SEAIEPV-DQAFSKWASKTHFTFPKISNYKKSDIKISF 227
           G+ +WP ++  L Y F P  P +E ++ V  +AF +W+  T   F    ++  SDI I F
Sbjct: 163 GEPRWPRNRRDLTYAFDPKNPLTEEVKSVFSRAFGRWSDVTALNFTLSESFSTSDITIGF 222

Query: 228 EVEEHGDFASLDGAGGIL 240
              +HGD    DG  G L
Sbjct: 223 YTGDHGDGEPFDGVLGTL 237

BLAST of MS003178 vs. TAIR 10
Match: AT4G16640.1 (Matrixin family protein )

HSP 1 Score: 110.5 bits (275), Expect = 1.8e-24
Identity = 80/238 (33.61%), Postives = 116/238 (48.74%), Query Frame = 0

Query: 9   FALILFLLLSFFPHPNM-SSHVLILKHNAIAHDMNSSSSLSFNNLRGCRLGDTAQGIHRL 68
           F LILF     F   N   +     K   I H  NS+    F+ L   ++G    G+  L
Sbjct: 15  FVLILFCFPYRFGARNTPEAEQSTAKATQIIHVSNSTWH-DFSRLVDVQIGSHVSGVSEL 74

Query: 69  KKYLRRFGYITNVEKNSMAMDDDTFDHALESAVKTYQKNYNIDASGILDAKTITQLGMPR 128
           K+YL RFGY+     +   +  D FD  LESA+  YQ+N  +  +G LD  T+T + +PR
Sbjct: 75  KRYLHRFGYV----NDGSEIFSDVFDGPLESAISLYQENLGLPITGRLDTSTVTLMSLPR 134

Query: 129 CGVQDFINGTTWMRSSMDHKNRDGRFHTVSHYSFFGGKAKWPASKFHLRYVF-----LPD 188
           CGV D     T M  + D        HT +HY++F GK KW  ++  L Y       L  
Sbjct: 135 CGVSD-----THMTINNDF------LHTTAHYTYFNGKPKW--NRDTLTYAISKTHKLDY 194

Query: 189 YPSEAIEPV-DQAFSKWASKTHFTFPKISNYKKSDIKISFEVEEHGDFASLDGAGGIL 240
             SE ++ V  +AFS+W+S    +F ++ ++  +D+KI F   +HGD    DG  G L
Sbjct: 195 LTSEDVKTVFRRAFSQWSSVIPVSFEEVDDFTTADLKIGFYAGDHGDGLPFDGVLGTL 234

BLAST of MS003178 vs. TAIR 10
Match: AT2G45040.1 (Matrixin family protein )

HSP 1 Score: 84.7 bits (208), Expect = 1.1e-16
Identity = 56/183 (30.60%), Postives = 90/183 (49.18%), Query Frame = 0

Query: 64  IHRLKKYLRRFGYITNVEKNSMAMDDDTFDHALESAVKTYQKNYNIDASGILDAKTITQL 123
           I  +K++L+++GY+   +++    DD +F    E A+  YQKN  +  +G  D+ T++Q+
Sbjct: 50  IPEIKRHLQQYGYLPQNKES----DDVSF----EQALVRYQKNLGLPITGKPDSDTLSQI 109

Query: 124 GMPRCGVQDFINGTTWMRSSMDHKNRDGRFHTVSHYSFFGGKAKWPAS-KFHLRYVFLPD 183
            +PRCG  D            D + +   FHT   Y +F G+ +W       L Y F  +
Sbjct: 110 LLPRCGFPD------------DVEPKTAPFHTGKKYVYFPGRPRWTRDVPLKLTYAFSQE 169

Query: 184 YPSEAIEPVD------QAFSKWASKTHFTFPKISNYKKSDIKISFEVEEHGDFASLDGAG 240
             +  + P D      +AF KWAS    +F +  +Y  +DIKI F   +HGD    DG  
Sbjct: 170 NLTPYLAPTDIRRVFRRAFGKWASVIPVSFIETEDYVIADIKIGFFNGDHGDGEPFDGVL 212

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022134118.14.4e-12190.79metalloendoproteinase 3-MMP-like [Momordica charantia] >AKQ06192.1 metalloendopr... [more]
XP_022134170.11.2e-11084.65metalloendoproteinase 2-MMP-like [Momordica charantia][more]
XP_022962668.15.6e-7661.26metalloendoproteinase 2-MMP-like [Cucurbita moschata][more]
XP_022991021.18.1e-7560.08metalloendoproteinase 2-MMP-like [Cucurbita maxima][more]
KAG6602462.13.4e-7360.47Metalloendoproteinase 2-MMP, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
Q5XF518.0e-3336.55Metalloendoproteinase 3-MMP OS=Arabidopsis thaliana OX=3702 GN=3MMP PE=1 SV=1[more]
Q9ZUJ58.9e-3239.18Metalloendoproteinase 5-MMP OS=Arabidopsis thaliana OX=3702 GN=5MMP PE=1 SV=1[more]
O045291.7e-3036.36Metalloendoproteinase 2-MMP OS=Arabidopsis thaliana OX=3702 GN=2MMP PE=1 SV=1[more]
P291364.3e-2636.41Metalloendoproteinase 1 OS=Glycine max OX=3847 PE=1 SV=2[more]
O235072.6e-2333.61Metalloendoproteinase 1-MMP OS=Arabidopsis thaliana OX=3702 GN=1MMP PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0H4TG972.1e-12190.79Metalloendoproteinase 1-like OS=Momordica charantia OX=3673 GN=LOC111006468 PE=2... [more]
A0A6J1BXD55.8e-11184.65metalloendoproteinase 2-MMP-like OS=Momordica charantia OX=3673 GN=LOC111006498 ... [more]
A0A6J1HFG12.7e-7661.26metalloendoproteinase 2-MMP-like OS=Cucurbita moschata OX=3662 GN=LOC111463086 P... [more]
A0A6J1JPK13.9e-7560.08metalloendoproteinase 2-MMP-like OS=Cucurbita maxima OX=3661 GN=LOC111487736 PE=... [more]
Q9LEL91.0e-6758.85Matrix metalloproteinase OS=Cucumis sativus OX=3659 GN=MMP-1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
AT1G24140.15.7e-3436.55Matrixin family protein [more]
AT1G59970.16.3e-3339.18Matrixin family protein [more]
AT1G70170.11.2e-3136.36matrix metalloproteinase [more]
AT4G16640.11.8e-2433.61Matrixin family protein [more]
AT2G45040.11.1e-1630.60Matrixin family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR021190Peptidase M10APRINTSPR00138MATRIXINcoord: 215..239
score: 42.22
coord: 123..136
score: 43.12
coord: 191..206
score: 32.7
IPR002477Peptidoglycan binding-likePFAMPF01471PG_binding_1coord: 62..123
e-value: 4.3E-12
score: 46.0
IPR024079Metallopeptidase, catalytic domain superfamilyGENE3D3.40.390.10Collagenase (Catalytic Domain)coord: 53..239
e-value: 2.6E-33
score: 117.4
IPR001818Peptidase M10, metallopeptidasePFAMPF00413Peptidase_M10coord: 186..239
e-value: 4.4E-9
score: 36.4
NoneNo IPR availablePANTHERPTHR10201MATRIX METALLOPROTEINASEcoord: 29..239
NoneNo IPR availablePANTHERPTHR10201:SF269METALLOENDOPROTEINASE 3-MMP-LIKEcoord: 29..239
NoneNo IPR availableSUPERFAMILY55486Metalloproteases ("zincins"), catalytic domaincoord: 161..239
IPR036365PGBD-like superfamilySUPERFAMILY47090PGBD-likecoord: 55..132

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS003178.1MS003178.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
cellular_component GO:0031012 extracellular matrix
molecular_function GO:0004222 metalloendopeptidase activity
molecular_function GO:0008237 metallopeptidase activity
molecular_function GO:0008270 zinc ion binding