MS003114 (gene) Bitter gourd (TR) v1

Overview
NameMS003114
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
Descriptionaltered inheritance of mitochondria protein 32-like
Locationscaffold234: 503420 .. 505967 (-)
RNA-Seq ExpressionMS003114
SyntenyMS003114
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCTCCGAGGATCTCTCCGCCGTTTCCAACGCCGATGACGAAAACTATGGATTCCGACGACAAGAGATGTATCAGAGCGACCTCGCCGGCACCGTCAACGCTTATGACCGGCACGTTTTTCTTTGCTATGAGAGTCCGGAGTCCTGGCCCTCCAATTTCGAAAAATCTGACTCCGACGTCCTCCCTAAGCTGCTATCTGCGGCTCTCAAGGATCGCAAGGATGATATCTCTGGGAAGGTTAGCGTTGACAGAATCGATGTTGTTCCACAATCGGAAATCCAATTTATTCGGAATAAGGCGTTTTCGGTTTGATTTTGTTGGAGTTGATGAATTTCGTGCTCGGTGCTTTTGTTTGTAACTTTGTCTTTTATTTGGTTTCTCAGACGAAGCTGACAGTATTCGCGGGTCGTGAAGAAACTGGATTTTCGGATGGAGATGTGTTGATTTTCCCCGAAATGGTTAAATACAGGTGACTTGCAAGGATTTAATGGAATTAGTTGTGGTTTCCGCAAAATTTCCGGGAGATGATAAATTACTGTCGACTTAGTTACTGGAAATATGCAGAAATATGCTGTCTGTTTCACGAGGATATAGTAATTCTTTCTATCGAATATTATGAAATCCTTGATTTCTTATTTATTTCTTCTCGCGTTGACTTTAACTTCTTTGGTTTCCAAGTAGATAATGAAGCTTAGGGTATTTTCCATTACTAGTTTCCTGAATCGGTATCATTTATGCACTAATCTGAAGCTAGAAAATTACTACAATCTTGGTTTTTGTATCTAGTATTTCTTATCAATTTGGGAGACCAGACTAGTAATGAATCAGTTAGATTTTGGAGGAATTGGAATGTTTACTCTTGTTATATGTGCCGCTCTTTCTTGAAGAGTGTTCTGCTGTGCTTTTGTTTCCATTTCCCTTCTTTCGTTTTTTTGTCTTGGTTTTCCTCTCGTAAGTTTTATTTTTTCTGTTTGGAATGTCAGGCATTTTCACTGTTTTTCCTTTTGATTTGATGATAATAGGGGTTTAAAAGAATCAGATGTGGATGGCTTTGTCAATGATGTGCTTGTGAATGATAAACCATGGGCATCCGGAGTGCAAGAGGTTTTGACTGGCTCTCATGTGTTTGTGTGTGTTCATGCTAGTCGAGATCGCAGATGTGGTGTCTGTGGACCCATTCTTGTTCATAAGTTTGATGAGGAGATTGAGTTGCGAGGACTAAAGGATCAGGTTTTGGTTAGTCCATGTTCACATGTTGGAGGACACAAGTATGCCGGAAACTTGATCATATACCAACATGGTGTGGATGGGAAAACTACAGGTCATTGGTAAGATTCTCCAAAGCTATTGTGGTGCTTAACATATTTACTGGAAAACGAACTCTAACCAAATATTTAAAATTTATGTGGGTTGAATTTGGGGTAAAACTTTATTGTTTATAGGTACGGATATGTAACTCCTGAAGATGTGCCTGGATTGCTTGAACAGCACATTGCCAAGGGCGAGGTTGTGGAAAGGCTTTTGAGGTTTGTGCTTTTCAAGTTACATTTTAATTCACGAACTGGATACTAAATTTATTTGAGCATTGCTGGTATGATAACAGCTACTAGTGAGTTATACGGAAAAATGCACTCTTTTTTATTGTTTCACTTCATGATTTTGTGGGATATACGTTTTGTATCAATAAACAATCTCTTCCTCTCAATATTTAATCATGTAGTTTAGGTTTACTGATCATGGACATAGTATCCTCCTCCCCAATTTTCATACGTTCTTTGGATCAGTGTCGTGAACTCTTAGAACTTAGTCTTCCGTTTACTGCTATGGAAGACTTCGTGTGGGCCTGAATGTCTGAGCTCTAAAGCAGATGATGCTGAGTGGACTTTTTGTTGTTAGTAGTTATTGTTTATGTTCAACACTCACCAATTTCCGTTGTCACCTTAACCCTCAGCGTCCCTCAGTAGATGGCCTAAATGGGTAGACTGCAATGTTTTCATTGGCTAGTTGACTGACTTGCTTTGGCTTCTCCATAGCTGTCCTGAGTCACCTATACTTTGGCTTCTCCAAAGAACAATCTATAAATTTCGAAGCAAGCAATTTCATTTTGCTTTCTGATTGCCTATTGATGCAATTATTCTGTTATTTGTCATTAAATTGGCATCACAATTTCAGAGGGCAAATGGGCGCAAGTCCTGAGGAAGGCAAGAAGGCAGGTGAACAAAATCTTCCAAATGGAGAAGATACAAAGAAAAGCAAGGTTGAAATTCAAGAGAATGGGGATCAAAGCAAAGTTGAAACAACAGCAGGTTGTTGCCAGGGTAGTAATGGATTCACTTGTTGCAGAGATGAGACTTCTGGTTCTGGGAAAAATATCATAATCGAAGAAAATTCGAAAGAAGCTTCAGAAGATCAGGTACCGAAGAAGGCGAGTAAATCATGTTGTTGGGCAGGAAAATGGGAACAAAGTGAAATTCTTGCTGCTGTTGCGATTGTTGGAGCTGTGGCCACTGTTGCTGTTGCCTATAGCTTCTACAGGAGATCATCA

mRNA sequence

ATGGCCTCCGAGGATCTCTCCGCCGTTTCCAACGCCGATGACGAAAACTATGGATTCCGACGACAAGAGATGTATCAGAGCGACCTCGCCGGCACCGTCAACGCTTATGACCGGCACGTTTTTCTTTGCTATGAGAGTCCGGAGTCCTGGCCCTCCAATTTCGAAAAATCTGACTCCGACGTCCTCCCTAAGCTGCTATCTGCGGCTCTCAAGGATCGCAAGGATGATATCTCTGGGAAGACGAAGCTGACAGTATTCGCGGGTCGTGAAGAAACTGGATTTTCGGATGGAGATGTGTTGATTTTCCCCGAAATGGTTAAATACAGGGGTTTAAAAGAATCAGATGTGGATGGCTTTGTCAATGATGTGCTTGTGAATGATAAACCATGGGCATCCGGAGTGCAAGAGGTTTTGACTGGCTCTCATGTGTTTGTGTGTGTTCATGCTAGTCGAGATCGCAGATGTGGTGTCTGTGGACCCATTCTTGTTCATAAGTTTGATGAGGAGATTGAGTTGCGAGGACTAAAGGATCAGGTTTTGGTTAGTCCATGTTCACATGTTGGAGGACACAAGTATGCCGGAAACTTGATCATATACCAACATGGTGTGGATGGGAAAACTACAGGTCATTGGTACGGATATGTAACTCCTGAAGATGTGCCTGGATTGCTTGAACAGCACATTGCCAAGGGCGAGGTTGTGGAAAGGCTTTTGAGAGGGCAAATGGGCGCAAGTCCTGAGGAAGGCAAGAAGGCAGGTGAACAAAATCTTCCAAATGGAGAAGATACAAAGAAAAGCAAGGTTGAAATTCAAGAGAATGGGGATCAAAGCAAAGTTGAAACAACAGCAGGTTGTTGCCAGGGTAGTAATGGATTCACTTGTTGCAGAGATGAGACTTCTGGTTCTGGGAAAAATATCATAATCGAAGAAAATTCGAAAGAAGCTTCAGAAGATCAGGTACCGAAGAAGGCGAGTAAATCATGTTGTTGGGCAGGAAAATGGGAACAAAGTGAAATTCTTGCTGCTGTTGCGATTGTTGGAGCTGTGGCCACTGTTGCTGTTGCCTATAGCTTCTACAGGAGATCATCA

Coding sequence (CDS)

ATGGCCTCCGAGGATCTCTCCGCCGTTTCCAACGCCGATGACGAAAACTATGGATTCCGACGACAAGAGATGTATCAGAGCGACCTCGCCGGCACCGTCAACGCTTATGACCGGCACGTTTTTCTTTGCTATGAGAGTCCGGAGTCCTGGCCCTCCAATTTCGAAAAATCTGACTCCGACGTCCTCCCTAAGCTGCTATCTGCGGCTCTCAAGGATCGCAAGGATGATATCTCTGGGAAGACGAAGCTGACAGTATTCGCGGGTCGTGAAGAAACTGGATTTTCGGATGGAGATGTGTTGATTTTCCCCGAAATGGTTAAATACAGGGGTTTAAAAGAATCAGATGTGGATGGCTTTGTCAATGATGTGCTTGTGAATGATAAACCATGGGCATCCGGAGTGCAAGAGGTTTTGACTGGCTCTCATGTGTTTGTGTGTGTTCATGCTAGTCGAGATCGCAGATGTGGTGTCTGTGGACCCATTCTTGTTCATAAGTTTGATGAGGAGATTGAGTTGCGAGGACTAAAGGATCAGGTTTTGGTTAGTCCATGTTCACATGTTGGAGGACACAAGTATGCCGGAAACTTGATCATATACCAACATGGTGTGGATGGGAAAACTACAGGTCATTGGTACGGATATGTAACTCCTGAAGATGTGCCTGGATTGCTTGAACAGCACATTGCCAAGGGCGAGGTTGTGGAAAGGCTTTTGAGAGGGCAAATGGGCGCAAGTCCTGAGGAAGGCAAGAAGGCAGGTGAACAAAATCTTCCAAATGGAGAAGATACAAAGAAAAGCAAGGTTGAAATTCAAGAGAATGGGGATCAAAGCAAAGTTGAAACAACAGCAGGTTGTTGCCAGGGTAGTAATGGATTCACTTGTTGCAGAGATGAGACTTCTGGTTCTGGGAAAAATATCATAATCGAAGAAAATTCGAAAGAAGCTTCAGAAGATCAGGTACCGAAGAAGGCGAGTAAATCATGTTGTTGGGCAGGAAAATGGGAACAAAGTGAAATTCTTGCTGCTGTTGCGATTGTTGGAGCTGTGGCCACTGTTGCTGTTGCCTATAGCTTCTACAGGAGATCATCA

Protein sequence

MASEDLSAVSNADDENYGFRRQEMYQSDLAGTVNAYDRHVFLCYESPESWPSNFEKSDSDVLPKLLSAALKDRKDDISGKTKLTVFAGREETGFSDGDVLIFPEMVKYRGLKESDVDGFVNDVLVNDKPWASGVQEVLTGSHVFVCVHASRDRRCGVCGPILVHKFDEEIELRGLKDQVLVSPCSHVGGHKYAGNLIIYQHGVDGKTTGHWYGYVTPEDVPGLLEQHIAKGEVVERLLRGQMGASPEEGKKAGEQNLPNGEDTKKSKVEIQENGDQSKVETTAGCCQGSNGFTCCRDETSGSGKNIIIEENSKEASEDQVPKKASKSCCWAGKWEQSEILAAVAIVGAVATVAVAYSFYRRSS
Homology
BLAST of MS003114 vs. NCBI nr
Match: XP_022134290.1 (uncharacterized protein LOC111006588 [Momordica charantia])

HSP 1 Score: 732.6 bits (1890), Expect = 1.6e-207
Identity = 361/363 (99.45%), Postives = 362/363 (99.72%), Query Frame = 0

Query: 1   MASEDLSAVSNADDENYGFRRQEMYQSDLAGTVNAYDRHVFLCYESPESWPSNFEKSDSD 60
           MASEDLSAVSNADDENYGFRRQEMYQSDLAGTVNAYDRHVFLCYESPESWPSNFEKSDSD
Sbjct: 1   MASEDLSAVSNADDENYGFRRQEMYQSDLAGTVNAYDRHVFLCYESPESWPSNFEKSDSD 60

Query: 61  VLPKLLSAALKDRKDDISGKTKLTVFAGREETGFSDGDVLIFPEMVKYRGLKESDVDGFV 120
           VLPKLLSAALKDRKDDISGKTKLTVFAGREETGFSDGDVLIFPEMVKYRGLKESDVDGFV
Sbjct: 61  VLPKLLSAALKDRKDDISGKTKLTVFAGREETGFSDGDVLIFPEMVKYRGLKESDVDGFV 120

Query: 121 NDVLVNDKPWASGVQEVLTGSHVFVCVHASRDRRCGVCGPILVHKFDEEIELRGLKDQVL 180
           +DVLVNDKPWASGVQEVLTGSHVFVCVHASRDRRCGVCGPILVHKFDEEIELRGLKDQVL
Sbjct: 121 DDVLVNDKPWASGVQEVLTGSHVFVCVHASRDRRCGVCGPILVHKFDEEIELRGLKDQVL 180

Query: 181 VSPCSHVGGHKYAGNLIIYQHGVDGKTTGHWYGYVTPEDVPGLLEQHIAKGEVVERLLRG 240
           VSPCSHVGGHKYAGNLIIYQHGVDGKTTGHWYGYVTPEDVPGLLEQHIAKGEVVERLLRG
Sbjct: 181 VSPCSHVGGHKYAGNLIIYQHGVDGKTTGHWYGYVTPEDVPGLLEQHIAKGEVVERLLRG 240

Query: 241 QMGASPEEGKKAGEQNLPNGEDTKKSKVEIQENGDQSKVETTAGCCQGSNGFTCCRDETS 300
           QMGASPEEGKKAGEQNLPNGEDTKKSKVEIQENGDQSKVE TAGCCQGSNGFTCCRDETS
Sbjct: 241 QMGASPEEGKKAGEQNLPNGEDTKKSKVEIQENGDQSKVEKTAGCCQGSNGFTCCRDETS 300

Query: 301 GSGKNIIIEENSKEASEDQVPKKASKSCCWAGKWEQSEILAAVAIVGAVATVAVAYSFYR 360
           GSGKNIIIEENSKEASEDQVPKKASKSCCWAGKWEQSEILAAVAIVGAVATVAVAYSFYR
Sbjct: 301 GSGKNIIIEENSKEASEDQVPKKASKSCCWAGKWEQSEILAAVAIVGAVATVAVAYSFYR 360

Query: 361 RSS 364
           RSS
Sbjct: 361 RSS 363

BLAST of MS003114 vs. NCBI nr
Match: KAG7016209.1 (AIM32 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 604.7 bits (1558), Expect = 5.1e-169
Identity = 302/363 (83.20%), Postives = 326/363 (89.81%), Query Frame = 0

Query: 1   MASEDLSAVSNADDENYGFRRQEMYQSDLAGTVNAYDRHVFLCYESPESWPSNFEKSDSD 60
           MAS++LS +S ADDENYGFRR+EMYQS LAGTV+AYDRHVFLCY++PE+WPS+ E SDSD
Sbjct: 1   MASDNLS-ISVADDENYGFRREEMYQSKLAGTVSAYDRHVFLCYKTPEAWPSHLEASDSD 60

Query: 61  VLPKLLSAALKDRKDDISGKTKLTVFAGREETGFSDGDVLIFPEMVKYRGLKESDVDGFV 120
           VLP+LLSAALK RKDD+S KTKLT+F+GREETGFSDGDVLIFPEMVKYRGL++SDVDGFV
Sbjct: 61  VLPRLLSAALKARKDDLSVKTKLTIFSGREETGFSDGDVLIFPEMVKYRGLEDSDVDGFV 120

Query: 121 NDVLVNDKPWASGVQEVLTGSHVFVCVHASRDRRCGVCGPILVHKFDEEIELRGLKDQVL 180
           +DVLVNDKPWAS   EV TGSHVFVCVHASRDRRCGVCGPIL+ KF+EEI LRGLKDQV 
Sbjct: 121 DDVLVNDKPWASREPEVFTGSHVFVCVHASRDRRCGVCGPILIQKFEEEIGLRGLKDQVY 180

Query: 181 VSPCSHVGGHKYAGNLIIYQHGVDGKTTGHWYGYVTPEDVPGLLEQHIAKGEVVERLLRG 240
           VSPCSHVGGHKYAGNLIIYQ GVDGKTTGHWYGYVTPEDV  L EQHIAKGEVVERLLRG
Sbjct: 181 VSPCSHVGGHKYAGNLIIYQPGVDGKTTGHWYGYVTPEDVSELFEQHIAKGEVVERLLRG 240

Query: 241 QMGASPEEGKKAGEQNLPNGEDTKKSKVEIQENGDQSKVETTAGCCQGSNGFTCCRDETS 300
           QMG +PEE +K  EQ LPNGEDTKKSK EIQENGDQSKVET AGCCQGSNGFTCCRDE+ 
Sbjct: 241 QMGTNPEEVQKEVEQKLPNGEDTKKSKDEIQENGDQSKVETVAGCCQGSNGFTCCRDES- 300

Query: 301 GSGKNIIIEENSKEASEDQVPKKASK-SCCWAGKWEQSEILAAVAIVGAVATVAVAYSFY 360
            SGK+  IEE SKE SEDQVPKK SK SCCW  KWEQSEIL AVA++GAVATVAVAYS Y
Sbjct: 301 -SGKSTRIEETSKEPSEDQVPKKTSKLSCCWTEKWEQSEILTAVAVIGAVATVAVAYSIY 360

Query: 361 RRS 363
           RRS
Sbjct: 361 RRS 360

BLAST of MS003114 vs. NCBI nr
Match: XP_038890361.1 (altered inheritance of mitochondria protein 32-like [Benincasa hispida])

HSP 1 Score: 603.2 bits (1554), Expect = 1.5e-168
Identity = 295/362 (81.49%), Postives = 322/362 (88.95%), Query Frame = 0

Query: 1   MASEDLSAVSNADDENYGFRRQEMYQSDLAGTVNAYDRHVFLCYESPESWPSNFEKSDSD 60
           MAS++LS +S ADDENYGFRR+EMYQS+LAGTV+AYDRHVFLCY++PE WPS+ E SDSD
Sbjct: 1   MASDNLSTLSGADDENYGFRREEMYQSNLAGTVSAYDRHVFLCYKTPEDWPSHLESSDSD 60

Query: 61  VLPKLLSAALKDRKDDISGKTKLTVFAGREETGFSDGDVLIFPEMVKYRGLKESDVDGFV 120
           VLP+LLSAALK RKDDIS KTKLT+F+GREETGFSDGDVLIFPEM+KYRGLK+SDVDGFV
Sbjct: 61  VLPRLLSAALKARKDDISLKTKLTIFSGREETGFSDGDVLIFPEMIKYRGLKDSDVDGFV 120

Query: 121 NDVLVNDKPWASGVQEVLTGSHVFVCVHASRDRRCGVCGPILVHKFDEEIELRGLKDQVL 180
           +DVLVNDKPWASG  E+ TGSHVFVCVHASRDRRCGVCGP+LV KFDEEI LR +KDQV 
Sbjct: 121 DDVLVNDKPWASGAPEIFTGSHVFVCVHASRDRRCGVCGPVLVQKFDEEIGLREMKDQVY 180

Query: 181 VSPCSHVGGHKYAGNLIIYQHGVDGKTTGHWYGYVTPEDVPGLLEQHIAKGEVVERLLRG 240
           VSPCSH+GGHKYAGNLIIYQ GVDGKTTGHWYGYVTPEDV  L E+HIAKGEVVERLLRG
Sbjct: 181 VSPCSHIGGHKYAGNLIIYQPGVDGKTTGHWYGYVTPEDVSELFERHIAKGEVVERLLRG 240

Query: 241 QMGASPEEGKKAGEQNLPNGEDTKKSKVEIQENGDQSKVETTAGCCQGSNGFTCCRDETS 300
           QMG +PEE +K GE+ LPNGEDTK+SKVEIQENGDQS VET  GC QGSNGFTCCRDE+ 
Sbjct: 241 QMGTNPEEVQKEGERKLPNGEDTKRSKVEIQENGDQSIVETFTGCSQGSNGFTCCRDES- 300

Query: 301 GSGKNIIIEENSKEASEDQVPKKASKSCCWAGKWEQSEILAAVAIVGAVATVAVAYSFYR 360
            S K   IEE SKE SEDQVPK  SK  CW GKWEQSEILAAVA++GAVATVAVAYS YR
Sbjct: 301 -SAKTSRIEEKSKEISEDQVPKMGSKFSCWTGKWEQSEILAAVAVIGAVATVAVAYSIYR 360

Query: 361 RS 363
           RS
Sbjct: 361 RS 360

BLAST of MS003114 vs. NCBI nr
Match: KAG6578669.1 (hypothetical protein SDJN03_23117, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 602.1 bits (1551), Expect = 3.3e-168
Identity = 300/363 (82.64%), Postives = 326/363 (89.81%), Query Frame = 0

Query: 1   MASEDLSAVSNADDENYGFRRQEMYQSDLAGTVNAYDRHVFLCYESPESWPSNFEKSDSD 60
           MAS++LS +S ADDENYGFRR+EMYQS LAGTV+AYDRHVFLCY++PE+WPS+ E SDSD
Sbjct: 1   MASDNLS-ISVADDENYGFRREEMYQSKLAGTVSAYDRHVFLCYKTPEAWPSHLEASDSD 60

Query: 61  VLPKLLSAALKDRKDDISGKTKLTVFAGREETGFSDGDVLIFPEMVKYRGLKESDVDGFV 120
           VLP+LLSAALK RKDD+S KTKLT+F+GREETGFSDGDVLIFPEMVKYRGL++SDVDGFV
Sbjct: 61  VLPRLLSAALKARKDDLSVKTKLTIFSGREETGFSDGDVLIFPEMVKYRGLEDSDVDGFV 120

Query: 121 NDVLVNDKPWASGVQEVLTGSHVFVCVHASRDRRCGVCGPILVHKFDEEIELRGLKDQVL 180
           +DVLVNDKPWAS   EV TGSHVFVCVHASRDRRCGVCGPIL+ KF+EEI LRGLKD+V 
Sbjct: 121 DDVLVNDKPWASREPEVFTGSHVFVCVHASRDRRCGVCGPILIQKFEEEIGLRGLKDRVY 180

Query: 181 VSPCSHVGGHKYAGNLIIYQHGVDGKTTGHWYGYVTPEDVPGLLEQHIAKGEVVERLLRG 240
           VSPCSHVGGHKYAGNLIIYQ GVDGKTTGHWYGYVTPEDV  L EQHIAKGEV+ERLLRG
Sbjct: 181 VSPCSHVGGHKYAGNLIIYQPGVDGKTTGHWYGYVTPEDVSELFEQHIAKGEVLERLLRG 240

Query: 241 QMGASPEEGKKAGEQNLPNGEDTKKSKVEIQENGDQSKVETTAGCCQGSNGFTCCRDETS 300
           QMG +PEE +K  EQ LPNGEDTKKSK EIQENGDQSKVET AGCCQGSNGFTCCRDE+ 
Sbjct: 241 QMGTNPEEVQKEVEQKLPNGEDTKKSKDEIQENGDQSKVETVAGCCQGSNGFTCCRDES- 300

Query: 301 GSGKNIIIEENSKEASEDQVPKKASK-SCCWAGKWEQSEILAAVAIVGAVATVAVAYSFY 360
            SGK+  IEE SKE SEDQVPKK SK SCCW  KWEQSEIL AVA++GAVATVAVAYS Y
Sbjct: 301 -SGKSTRIEETSKEPSEDQVPKKTSKLSCCWTEKWEQSEILTAVAVIGAVATVAVAYSIY 360

Query: 361 RRS 363
           RRS
Sbjct: 361 RRS 360

BLAST of MS003114 vs. NCBI nr
Match: XP_022939649.1 (altered inheritance of mitochondria protein 32-like [Cucurbita moschata])

HSP 1 Score: 601.7 bits (1550), Expect = 4.3e-168
Identity = 300/363 (82.64%), Postives = 325/363 (89.53%), Query Frame = 0

Query: 1   MASEDLSAVSNADDENYGFRRQEMYQSDLAGTVNAYDRHVFLCYESPESWPSNFEKSDSD 60
           MAS++LS +S ADDENYGFRR+EMYQS L GTV+AYDRHVFLCY++PE+WPS+ E SDSD
Sbjct: 1   MASDNLS-ISVADDENYGFRREEMYQSKLTGTVSAYDRHVFLCYKTPEAWPSHLEASDSD 60

Query: 61  VLPKLLSAALKDRKDDISGKTKLTVFAGREETGFSDGDVLIFPEMVKYRGLKESDVDGFV 120
           VLP+LLSAALK RKDD+S KTKLT+F+GREETGFSDGDVLIFPEMVKYRGL++SDVDGFV
Sbjct: 61  VLPRLLSAALKARKDDLSVKTKLTIFSGREETGFSDGDVLIFPEMVKYRGLEDSDVDGFV 120

Query: 121 NDVLVNDKPWASGVQEVLTGSHVFVCVHASRDRRCGVCGPILVHKFDEEIELRGLKDQVL 180
           +DVLVNDKPWAS   EV TGSHVFVCVHASRDRRCGVCGPIL+ KF+EEI LRGLKD+V 
Sbjct: 121 DDVLVNDKPWASREPEVFTGSHVFVCVHASRDRRCGVCGPILIQKFEEEIGLRGLKDRVY 180

Query: 181 VSPCSHVGGHKYAGNLIIYQHGVDGKTTGHWYGYVTPEDVPGLLEQHIAKGEVVERLLRG 240
           VSPCSHVGGHKYAGNLIIYQ GVDGKTTGHWYGYVTPEDV  L EQHIAKGEVVERLLRG
Sbjct: 181 VSPCSHVGGHKYAGNLIIYQPGVDGKTTGHWYGYVTPEDVSELFEQHIAKGEVVERLLRG 240

Query: 241 QMGASPEEGKKAGEQNLPNGEDTKKSKVEIQENGDQSKVETTAGCCQGSNGFTCCRDETS 300
           QMG +PEE +K  EQ LPNGEDTKKSK EIQENGDQSKVET AGCCQGSNGFTCCRDE+ 
Sbjct: 241 QMGTNPEEVQKEVEQKLPNGEDTKKSKDEIQENGDQSKVETVAGCCQGSNGFTCCRDES- 300

Query: 301 GSGKNIIIEENSKEASEDQVPKKASK-SCCWAGKWEQSEILAAVAIVGAVATVAVAYSFY 360
            SGK+  IEE SKE SEDQVPKK SK SCCW  KWEQSEIL AVA++GAVATVAVAYS Y
Sbjct: 301 -SGKSTRIEETSKEPSEDQVPKKTSKLSCCWTEKWEQSEILTAVAVIGAVATVAVAYSIY 360

Query: 361 RRS 363
           RRS
Sbjct: 361 RRS 360

BLAST of MS003114 vs. ExPASy Swiss-Prot
Match: A5DK49 (Altered inheritance of mitochondria protein 32 OS=Meyerozyma guilliermondii (strain ATCC 6260 / CBS 566 / DSM 6381 / JCM 1539 / NBRC 10279 / NRRL Y-324) OX=294746 GN=AIM32 PE=3 SV=2)

HSP 1 Score: 89.0 bits (219), Expect = 1.2e-16
Identity = 40/111 (36.04%), Postives = 61/111 (54.95%), Query Frame = 0

Query: 135 QEVLTGSHVFVCVHASRDRRCGVCGPILVHKFDEEIELRGLKDQVLVSPCSHVGGHKYAG 194
           ++ +T   V +C H SRD RCGV GP+L  +FD+ +    +   V     +HVGGH YAG
Sbjct: 176 EQDITDELVLICGHGSRDVRCGVMGPLLQREFDQVLTQENMSSHVKTGQITHVGGHAYAG 235

Query: 195 NLIIYQHGVDGKTTGHWYGYVTPEDVPGLLEQHIAKGEVVERLLRGQMGAS 246
           N++ +      K    WYG V PEDV G+++  I +G ++    RG +  +
Sbjct: 236 NVVYFPR----KGESVWYGRVFPEDVQGIVDTTIKQGVIIRDKYRGYVNGA 282

BLAST of MS003114 vs. ExPASy Swiss-Prot
Match: Q6BPT6 (Altered inheritance of mitochondria protein 32 OS=Debaryomyces hansenii (strain ATCC 36239 / CBS 767 / BCRC 21394 / JCM 1990 / NBRC 0083 / IGC 2968) OX=284592 GN=AIM32 PE=3 SV=2)

HSP 1 Score: 84.7 bits (208), Expect = 2.3e-15
Identity = 41/100 (41.00%), Postives = 60/100 (60.00%), Query Frame = 0

Query: 143 VFVCVHASRDRRCGVCGPILVHKFDEEIELRGLKDQVLVSPCSHVGGHKYAGNLIIYQHG 202
           + VC H  RD RCG   PILV +F + ++ RGL   V V   SH+GGH YAGN+I +   
Sbjct: 178 ILVCGHTQRDVRCGKIAPILVDQFKKSLKDRGL--DVDVGVVSHIGGHAYAGNVIYFPSD 237

Query: 203 VDGKTTGHWYGYVTPEDVPGLLEQHIAKGEVVERLLRGQM 243
              K+   WYG V PE V G++++ +  G +++ L RG++
Sbjct: 238 KTKKSI--WYGRVFPEKVAGIVDETVIGGNIIKELYRGEV 273

BLAST of MS003114 vs. ExPASy Swiss-Prot
Match: P38281 (Actin patches distal protein 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=APD1 PE=1 SV=1)

HSP 1 Score: 79.3 bits (194), Expect = 9.7e-14
Identity = 51/156 (32.69%), Postives = 76/156 (48.72%), Query Frame = 0

Query: 98  DVLIFPEMVKYRGLKESDV----DGFVNDV---------LVNDKPWASGVQEVLTGSHVF 157
           +VLI P  +    L+  DV    DG V D+         L+  +P  +  +E    + VF
Sbjct: 149 NVLILPYFIWLNDLRSDDVEATLDGLVPDLLDENISREKLLETRPNVAVARE---RAFVF 208

Query: 158 VCVHASRDRRCGVCGPILVHKFDEEIELRGL--------KDQVLVSPCSHVGGHKYAGNL 217
           +C H +RD+RCG+  P L   FD +++  GL         + V ++  +HVGGHK+A N+
Sbjct: 209 ICSHTTRDKRCGITAPYLKKVFDSKLQEHGLYRDNSDYRAEGVKIAFVNHVGGHKFAANV 268

Query: 218 IIYQHGVDGKTTGHWYGYVTPEDVPGLLEQHIAKGE 233
            IY   +    T  W G VTP  VP ++E  I   E
Sbjct: 269 QIY---LRNPNTLIWLGRVTPTIVPSIVEHLIVPEE 298

BLAST of MS003114 vs. ExPASy Swiss-Prot
Match: C4Y1G7 (Altered inheritance of mitochondria protein 32 OS=Clavispora lusitaniae (strain ATCC 42720) OX=306902 GN=AIM32 PE=3 SV=1)

HSP 1 Score: 76.3 bits (186), Expect = 8.2e-13
Identity = 38/99 (38.38%), Postives = 54/99 (54.55%), Query Frame = 0

Query: 143 VFVCVHASRDRRCGVCGPILVHKFDEEIELRGLKDQVLVSPCSHVGGHKYAGNLIIYQHG 202
           V +C H  RD RCG   P+L+ +F+  +    L   V V   SH+GGH YAGN+I +   
Sbjct: 179 VLICGHTQRDIRCGRIAPLLLQEFERVLAHEKL--DVDVGLVSHIGGHAYAGNVIYFSK- 238

Query: 203 VDGKTTGHWYGYVTPEDVPGLLEQHIAKGEVVERLLRGQ 242
                   WYG V PE V G++ + I +G +++ L RGQ
Sbjct: 239 ---HQPPVWYGRVFPEQVQGIVRETIVEGRIIKELYRGQ 271

BLAST of MS003114 vs. ExPASy Swiss-Prot
Match: Q6FLQ5 (Altered inheritance of mitochondria protein 32 OS=Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65) OX=284593 GN=AIM32 PE=3 SV=1)

HSP 1 Score: 73.6 bits (179), Expect = 5.3e-12
Identity = 69/261 (26.44%), Postives = 114/261 (43.68%), Query Frame = 0

Query: 29  LAGTVNAYDRHVFLCYESPES-----------WPSNFEKSDS---DVLPKLLSAALKDRK 88
           L   V +YD+HV L  +               W S  E + +   D++ K L+       
Sbjct: 59  LKTAVPSYDKHVMLISDINRGMAKKPGVWKNIWESRIENNTTHPYDIISK-LNFGPGVLF 118

Query: 89  DDISGKTKLTVFAGREETGFSDGDVLIFPEMVKYRGLKESDVDGFVNDV----------- 148
           + IS  + L  FA    T     D L+ P+M  YR +K+ D++ F   +           
Sbjct: 119 NAISITSSLESFA---PTSLEFYDFLVMPDMRYYR-VKKPDIEKFSQYINSGHAVAPKLS 178

Query: 149 ---LVNDKPWASGV----------------QEVLTGSHVFVCVHASRDRRCGVCGPILVH 208
               ++ K  A+ V                +E+   + +FVC H  RD RCG+ GP ++H
Sbjct: 179 FSDYLSGKAAATTVSNNNQITLSLDDSIYYRELKNDAWLFVCGHEKRDMRCGIMGPEILH 238

Query: 209 KFDEEIELRGLKDQVLVSPCSHVGGHKYAGNLIIY-----QHGVDGKTTGHWYGYVTPED 241
             +       + +  ++   SH+GGHK+AGN++IY     Q+G   K    W+G VTP +
Sbjct: 239 SVNTANSKPLVNNTGII---SHIGGHKFAGNILIYKPIENQNG-RKKVDSLWFGKVTPFN 298

BLAST of MS003114 vs. ExPASy TrEMBL
Match: A0A6J1BXW9 (uncharacterized protein LOC111006588 OS=Momordica charantia OX=3673 GN=LOC111006588 PE=4 SV=1)

HSP 1 Score: 732.6 bits (1890), Expect = 7.8e-208
Identity = 361/363 (99.45%), Postives = 362/363 (99.72%), Query Frame = 0

Query: 1   MASEDLSAVSNADDENYGFRRQEMYQSDLAGTVNAYDRHVFLCYESPESWPSNFEKSDSD 60
           MASEDLSAVSNADDENYGFRRQEMYQSDLAGTVNAYDRHVFLCYESPESWPSNFEKSDSD
Sbjct: 1   MASEDLSAVSNADDENYGFRRQEMYQSDLAGTVNAYDRHVFLCYESPESWPSNFEKSDSD 60

Query: 61  VLPKLLSAALKDRKDDISGKTKLTVFAGREETGFSDGDVLIFPEMVKYRGLKESDVDGFV 120
           VLPKLLSAALKDRKDDISGKTKLTVFAGREETGFSDGDVLIFPEMVKYRGLKESDVDGFV
Sbjct: 61  VLPKLLSAALKDRKDDISGKTKLTVFAGREETGFSDGDVLIFPEMVKYRGLKESDVDGFV 120

Query: 121 NDVLVNDKPWASGVQEVLTGSHVFVCVHASRDRRCGVCGPILVHKFDEEIELRGLKDQVL 180
           +DVLVNDKPWASGVQEVLTGSHVFVCVHASRDRRCGVCGPILVHKFDEEIELRGLKDQVL
Sbjct: 121 DDVLVNDKPWASGVQEVLTGSHVFVCVHASRDRRCGVCGPILVHKFDEEIELRGLKDQVL 180

Query: 181 VSPCSHVGGHKYAGNLIIYQHGVDGKTTGHWYGYVTPEDVPGLLEQHIAKGEVVERLLRG 240
           VSPCSHVGGHKYAGNLIIYQHGVDGKTTGHWYGYVTPEDVPGLLEQHIAKGEVVERLLRG
Sbjct: 181 VSPCSHVGGHKYAGNLIIYQHGVDGKTTGHWYGYVTPEDVPGLLEQHIAKGEVVERLLRG 240

Query: 241 QMGASPEEGKKAGEQNLPNGEDTKKSKVEIQENGDQSKVETTAGCCQGSNGFTCCRDETS 300
           QMGASPEEGKKAGEQNLPNGEDTKKSKVEIQENGDQSKVE TAGCCQGSNGFTCCRDETS
Sbjct: 241 QMGASPEEGKKAGEQNLPNGEDTKKSKVEIQENGDQSKVEKTAGCCQGSNGFTCCRDETS 300

Query: 301 GSGKNIIIEENSKEASEDQVPKKASKSCCWAGKWEQSEILAAVAIVGAVATVAVAYSFYR 360
           GSGKNIIIEENSKEASEDQVPKKASKSCCWAGKWEQSEILAAVAIVGAVATVAVAYSFYR
Sbjct: 301 GSGKNIIIEENSKEASEDQVPKKASKSCCWAGKWEQSEILAAVAIVGAVATVAVAYSFYR 360

Query: 361 RSS 364
           RSS
Sbjct: 361 RSS 363

BLAST of MS003114 vs. ExPASy TrEMBL
Match: A0A6J1FHU3 (altered inheritance of mitochondria protein 32-like OS=Cucurbita moschata OX=3662 GN=LOC111445480 PE=4 SV=1)

HSP 1 Score: 601.7 bits (1550), Expect = 2.1e-168
Identity = 300/363 (82.64%), Postives = 325/363 (89.53%), Query Frame = 0

Query: 1   MASEDLSAVSNADDENYGFRRQEMYQSDLAGTVNAYDRHVFLCYESPESWPSNFEKSDSD 60
           MAS++LS +S ADDENYGFRR+EMYQS L GTV+AYDRHVFLCY++PE+WPS+ E SDSD
Sbjct: 1   MASDNLS-ISVADDENYGFRREEMYQSKLTGTVSAYDRHVFLCYKTPEAWPSHLEASDSD 60

Query: 61  VLPKLLSAALKDRKDDISGKTKLTVFAGREETGFSDGDVLIFPEMVKYRGLKESDVDGFV 120
           VLP+LLSAALK RKDD+S KTKLT+F+GREETGFSDGDVLIFPEMVKYRGL++SDVDGFV
Sbjct: 61  VLPRLLSAALKARKDDLSVKTKLTIFSGREETGFSDGDVLIFPEMVKYRGLEDSDVDGFV 120

Query: 121 NDVLVNDKPWASGVQEVLTGSHVFVCVHASRDRRCGVCGPILVHKFDEEIELRGLKDQVL 180
           +DVLVNDKPWAS   EV TGSHVFVCVHASRDRRCGVCGPIL+ KF+EEI LRGLKD+V 
Sbjct: 121 DDVLVNDKPWASREPEVFTGSHVFVCVHASRDRRCGVCGPILIQKFEEEIGLRGLKDRVY 180

Query: 181 VSPCSHVGGHKYAGNLIIYQHGVDGKTTGHWYGYVTPEDVPGLLEQHIAKGEVVERLLRG 240
           VSPCSHVGGHKYAGNLIIYQ GVDGKTTGHWYGYVTPEDV  L EQHIAKGEVVERLLRG
Sbjct: 181 VSPCSHVGGHKYAGNLIIYQPGVDGKTTGHWYGYVTPEDVSELFEQHIAKGEVVERLLRG 240

Query: 241 QMGASPEEGKKAGEQNLPNGEDTKKSKVEIQENGDQSKVETTAGCCQGSNGFTCCRDETS 300
           QMG +PEE +K  EQ LPNGEDTKKSK EIQENGDQSKVET AGCCQGSNGFTCCRDE+ 
Sbjct: 241 QMGTNPEEVQKEVEQKLPNGEDTKKSKDEIQENGDQSKVETVAGCCQGSNGFTCCRDES- 300

Query: 301 GSGKNIIIEENSKEASEDQVPKKASK-SCCWAGKWEQSEILAAVAIVGAVATVAVAYSFY 360
            SGK+  IEE SKE SEDQVPKK SK SCCW  KWEQSEIL AVA++GAVATVAVAYS Y
Sbjct: 301 -SGKSTRIEETSKEPSEDQVPKKTSKLSCCWTEKWEQSEILTAVAVIGAVATVAVAYSIY 360

Query: 361 RRS 363
           RRS
Sbjct: 361 RRS 360

BLAST of MS003114 vs. ExPASy TrEMBL
Match: A0A6J1JRQ9 (altered inheritance of mitochondria protein 32-like OS=Cucurbita maxima OX=3661 GN=LOC111487744 PE=4 SV=1)

HSP 1 Score: 594.0 bits (1530), Expect = 4.3e-166
Identity = 291/362 (80.39%), Postives = 320/362 (88.40%), Query Frame = 0

Query: 1   MASEDLSAVSNADDENYGFRRQEMYQSDLAGTVNAYDRHVFLCYESPESWPSNFEKSDSD 60
           MAS++L+ VS ADDE +GFRR+EMYQS LAGTV+AYDRHVFLCY++PE+WPS+ E SDSD
Sbjct: 1   MASDNLATVSGADDEKFGFRREEMYQSSLAGTVSAYDRHVFLCYKTPEAWPSHLEASDSD 60

Query: 61  VLPKLLSAALKDRKDDISGKTKLTVFAGREETGFSDGDVLIFPEMVKYRGLKESDVDGFV 120
            LP+LLSAALK RKDDIS KTKLT+FAGREETGFSDGDVLIFPEMVKYRGLKESDVDGFV
Sbjct: 61  ALPRLLSAALKARKDDISVKTKLTMFAGREETGFSDGDVLIFPEMVKYRGLKESDVDGFV 120

Query: 121 NDVLVNDKPWASGVQEVLTGSHVFVCVHASRDRRCGVCGPILVHKFDEEIELRGLKDQVL 180
           +DVLVNDK WASGV +  TGSHVFVCVHASRDRRCGVCGP+LV K DEEIELRGLK++V 
Sbjct: 121 DDVLVNDKQWASGVPKAFTGSHVFVCVHASRDRRCGVCGPVLVQKLDEEIELRGLKERVF 180

Query: 181 VSPCSHVGGHKYAGNLIIYQHGVDGKTTGHWYGYVTPEDVPGLLEQHIAKGEVVERLLRG 240
           VS CSH+GGHKYAGNLIIYQ GVDGKTTGHWYGYVTPEDV  L E HIAKGEVVERLLRG
Sbjct: 181 VSACSHIGGHKYAGNLIIYQPGVDGKTTGHWYGYVTPEDVSELFELHIAKGEVVERLLRG 240

Query: 241 QMGASPEEGKKAGEQNLPNGEDTKKSKVEIQENGDQSKVETTAGCCQGSNGFTCCRDETS 300
           QMG +PEEGKK  E  +PNG+DTKKSKVEI+EN  Q+KVET AGCCQGSNGFTCCRDE+ 
Sbjct: 241 QMGTNPEEGKKEDELKIPNGDDTKKSKVEIEENSGQNKVETIAGCCQGSNGFTCCRDES- 300

Query: 301 GSGKNIIIEENSKEASEDQVPKKASKSCCWAGKWEQSEILAAVAIVGAVATVAVAYSFYR 360
            SGK+  IE  SKEASE+QVPKKA+K  CW G+WEQSEILAA A +GAVATVAVAYS YR
Sbjct: 301 -SGKSSRIENQSKEASEEQVPKKANKFSCWTGQWEQSEILAAAATIGAVATVAVAYSIYR 360

Query: 361 RS 363
           RS
Sbjct: 361 RS 360

BLAST of MS003114 vs. ExPASy TrEMBL
Match: A0A6J1JSI1 (altered inheritance of mitochondria protein 32-like OS=Cucurbita maxima OX=3661 GN=LOC111489377 PE=4 SV=1)

HSP 1 Score: 590.5 bits (1521), Expect = 4.8e-165
Identity = 295/363 (81.27%), Postives = 326/363 (89.81%), Query Frame = 0

Query: 1   MASEDLSAVSNADDENYGFRRQEMYQSDLAGTVNAYDRHVFLCYESPESWPSNFEKSDSD 60
           MAS++LS +S A+DENYGFRR+EMYQS+LAGTV+AYDRHVFLCY++PE+WPS+ E SDSD
Sbjct: 1   MASDNLS-ISVANDENYGFRREEMYQSNLAGTVSAYDRHVFLCYKTPEAWPSHLEASDSD 60

Query: 61  VLPKLLSAALKDRKDDISGKTKLTVFAGREETGFSDGDVLIFPEMVKYRGLKESDVDGFV 120
           VLP+LLSA+LK RKDD+S KTKLT+F+GREETGFSDGDVLIFPEMVKYRGL++SDVDGFV
Sbjct: 61  VLPRLLSASLKARKDDLSVKTKLTIFSGREETGFSDGDVLIFPEMVKYRGLEDSDVDGFV 120

Query: 121 NDVLVNDKPWASGVQEVLTGSHVFVCVHASRDRRCGVCGPILVHKFDEEIELRGLKDQVL 180
           +DVLVNDKPWAS   EV TGSHVFVCVHASRDRRCGVCGPIL+ KF+EEI LRGLKD+V 
Sbjct: 121 DDVLVNDKPWASREPEVFTGSHVFVCVHASRDRRCGVCGPILIQKFEEEIGLRGLKDRVY 180

Query: 181 VSPCSHVGGHKYAGNLIIYQHGVDGKTTGHWYGYVTPEDVPGLLEQHIAKGEVVERLLRG 240
           VSPCSHVGGHKYAGNLIIYQ GVDGKTTGHWYGYVTPEDV  L EQHIAKGEVVERLLRG
Sbjct: 181 VSPCSHVGGHKYAGNLIIYQPGVDGKTTGHWYGYVTPEDVSELFEQHIAKGEVVERLLRG 240

Query: 241 QMGASPEEGKKAGEQNLPNGEDTKKSKVEIQENGDQSKVETTAGCCQGSNGFTCCRDETS 300
           QMG +PEE +K  EQ LPNGEDT+K+K E+QENGDQSKVET AGCCQGSNGFTCCRDE+ 
Sbjct: 241 QMGTNPEEVQKEVEQKLPNGEDTRKNKDEVQENGDQSKVETVAGCCQGSNGFTCCRDES- 300

Query: 301 GSGKNIIIEENSKEAS-EDQVPKKASK-SCCWAGKWEQSEILAAVAIVGAVATVAVAYSF 360
            SGK+  IE  SKE S EDQVPKKASK SCCW  KWEQSEIL AVA++GAVATVAVAYS 
Sbjct: 301 -SGKSSRIEVKSKEPSEEDQVPKKASKFSCCWTEKWEQSEILTAVAVIGAVATVAVAYSI 360

Query: 361 YRR 362
           YRR
Sbjct: 361 YRR 360

BLAST of MS003114 vs. ExPASy TrEMBL
Match: A0A1S3C9A5 (altered inheritance of mitochondria protein 32-like OS=Cucumis melo OX=3656 GN=LOC103498108 PE=4 SV=1)

HSP 1 Score: 590.5 bits (1521), Expect = 4.8e-165
Identity = 293/364 (80.49%), Postives = 324/364 (89.01%), Query Frame = 0

Query: 1   MASEDLSAVSN-ADDENYGFRRQEMYQSDLAGTVNAYDRHVFLCYESPESWPSNFEKSDS 60
           MAS++LS+VS  ADD+NYGFRR+EMYQS+LAGTV+AYDRHVFLCY++PE+WPS+ E SD 
Sbjct: 42  MASDNLSSVSGAADDDNYGFRREEMYQSNLAGTVSAYDRHVFLCYKTPEAWPSHLESSDC 101

Query: 61  DVLPKLLSAALKDRKDDISGKTKLTVFAGREETGFSDGDVLIFPEMVKYRGLKESDVDGF 120
           D+LPKLLSAALK RKDDIS KTKLT+F+GR ETGFSDGD+LIFP+M+KY GLK+SDVDGF
Sbjct: 102 DLLPKLLSAALKARKDDISLKTKLTMFSGRYETGFSDGDLLIFPDMIKYSGLKDSDVDGF 161

Query: 121 VNDVLVNDKPWASGVQEVLTGSHVFVCVHASRDRRCGVCGPILVHKFDEEIELRGLKDQV 180
           V+DVLVN+KPWASGV EV TGSHVFVCVHASRDRRCGVCGPILV K DEEIELRGLKDQV
Sbjct: 162 VDDVLVNNKPWASGVPEVFTGSHVFVCVHASRDRRCGVCGPILVQKLDEEIELRGLKDQV 221

Query: 181 LVSPCSHVGGHKYAGNLIIYQHGVDGKTTGHWYGYVTPEDVPGLLEQHIAKGEVVERLLR 240
            VSPCSH+GGHKYAGNLIIYQ G DGKTTGHWYGYVTPED+  L EQHIAKGEVVERLLR
Sbjct: 222 YVSPCSHIGGHKYAGNLIIYQPGTDGKTTGHWYGYVTPEDLSELFEQHIAKGEVVERLLR 281

Query: 241 GQMGASPEEGKKAGEQNLPNGEDTKKSKVEIQENGDQSKVETTAGCCQGSNGFTCCRDET 300
           GQMG +PEE +K GEQ LPNGED+K+SKVEI+ENG+QSKVET A CCQGSNGFTCCRDE+
Sbjct: 282 GQMGTNPEEVQKEGEQKLPNGEDSKESKVEIKENGNQSKVETVASCCQGSNGFTCCRDES 341

Query: 301 SGSGKNIIIEENSKEASED-QVPKKASKSCCWAGKWEQSEILAAVAIVGAVATVAVAYSF 360
             SGK+  IEE SKE S D QVP  ASK  CW GKWEQSEIL AVA+VGAVATVAVAYS 
Sbjct: 342 --SGKSSSIEERSKEISNDEQVPTTASKFSCWTGKWEQSEILTAVAVVGAVATVAVAYSI 401

Query: 361 YRRS 363
           YRRS
Sbjct: 402 YRRS 403

BLAST of MS003114 vs. TAIR 10
Match: AT3G27570.1 (Sucrase/ferredoxin-like family protein )

HSP 1 Score: 356.3 bits (913), Expect = 2.9e-98
Identity = 179/362 (49.45%), Postives = 238/362 (65.75%), Query Frame = 0

Query: 3   SEDLSAVSNADDENYGFRRQEMYQSDLAGTVNAYDRHVFLCYESPESWPSNFEKSDSDVL 62
           +E+++AV  ++DE YGF+R EMY   LAG+V  Y RHVFLCY+S E+W     + +++ L
Sbjct: 45  AENVNAVPVSEDELYGFKRSEMYSGTLAGSVGPYGRHVFLCYKSHETW---LPRVETEGL 104

Query: 63  PKLLSAALKDRKDDISGKTKLTVFAGREETGFSDGDVLIFPEMVKYRGLKESDVDGFVND 122
           P+  +   KDRK D + +TKLTV  G    G SDGDVLIFPEMV+Y+ ++++DVD FV D
Sbjct: 105 PQRFAKLFKDRKADFAVETKLTVCGG---GGESDGDVLIFPEMVRYKAIQDTDVDAFVED 164

Query: 123 VLVNDKPWASGVQEVLTGSHVFVCVHASRDRRCGVCGPILVHKFDEEIELRGLKDQVLVS 182
           VLV  K W SG+QE LTGS VFVC H SRD+RCGVCGP+L+ KF++EI  RGL D++ V 
Sbjct: 165 VLVKGKTWTSGIQEELTGSFVFVCAHGSRDKRCGVCGPVLMEKFEQEISSRGLSDKIFVL 224

Query: 183 PCSHVGGHKYAGNLIIYQHGVDGKTTGHWYGYVTPEDVPGLLEQHIAKGEVVERLLRGQM 242
           PCSH+GGHKYAGNLI++     G  +GHWYGYVTP+DVP +L+QHIAKGE+++ L RGQM
Sbjct: 225 PCSHIGGHKYAGNLIVFSPDSAGNVSGHWYGYVTPDDVPAMLDQHIAKGEIIQNLSRGQM 284

Query: 243 GASP--EEGKKAGEQNLPNGEDTKKSKVEIQENGDQSKVETTAGCCQGSNGFTCCRDETS 302
              P  EE +K  E  +PNG     + V ++E     +   T GCCQG+NG +CC+++ +
Sbjct: 285 RLRPEGEEAEKEDEHKIPNG-----NSVMVEEREPVEQKGFTGGCCQGANGVSCCQEQAA 344

Query: 303 GSGKNIIIEENSKEASEDQVPKKASKSCCWAGKWEQSEILAAVAIVGAVATVAVAYSFYR 362
                            + V K+      W     + E+L   A+V AVATVAVAYS YR
Sbjct: 345 -----------------EPVKKEGCMKLNWLKSMGKEEVLLGAAVVSAVATVAVAYSIYR 378

BLAST of MS003114 vs. TAIR 10
Match: AT5G40510.1 (Sucrase/ferredoxin-like family protein )

HSP 1 Score: 347.4 bits (890), Expect = 1.4e-95
Identity = 173/360 (48.06%), Postives = 245/360 (68.06%), Query Frame = 0

Query: 4   EDLSAVSNADDENYGFRRQEMYQSDLAGTVNAYDRHVFLCYESPESWPSNFEKSDSDVLP 63
           E+L++V  ++D  YGF+R EMY +++A ++ +Y RHVF+ Y++PE+W S+ E+   + LP
Sbjct: 5   ENLNSVPASEDTEYGFKRPEMYSTNIANSITSYARHVFVLYKTPEAWLSHVEE---EGLP 64

Query: 64  KLLSAALKDRKDDISGKTKLTVFAGREETGFSDGDVLIFPEMVKYRGLKESDVDGFVNDV 123
           +  +  LKDRK D+  +TKL V  G    G SDGDVLIFP+M++Y+G+K++DV+GF  DV
Sbjct: 65  QRFATLLKDRKSDLLVQTKLNVCEG----GGSDGDVLIFPDMIRYKGVKDTDVEGFFEDV 124

Query: 124 LVNDKPWASGVQEVLTGSHVFVCVHASRDRRCGVCGPILVHKFDEEIELRGLKDQVLVSP 183
           LVN KPW+SG+QE ++G+ VFVC HASRD+RCGVCGP+++ +F +EI  RGL DQ+ +  
Sbjct: 125 LVNGKPWSSGIQEEISGTFVFVCTHASRDKRCGVCGPVILERFKQEIGSRGLSDQITLKR 184

Query: 184 CSHVGGHKYAGNLIIYQHGVDGKTTGHWYGYVTPEDVPGLLEQHIAKGEVVERLLRGQMG 243
           CSHVG HKYAGNLII+     GK TG+WYGYVTP+DVP LL+QHIAKGE+++R+ RGQMG
Sbjct: 185 CSHVGQHKYAGNLIIFCPDSAGKITGNWYGYVTPDDVPELLDQHIAKGEIIQRIWRGQMG 244

Query: 244 ASPEEGKKAGEQN-LPNGEDTKKSKVEIQENGDQSKVETTAGCCQGSNGFTCCRDETSGS 303
               E +K  EQ  +PNG    K         ++SK   T GCCQGSNG +CC+DET   
Sbjct: 245 LPGGEAEKVHEQKVIPNGHGVVK---------EESK-GFTGGCCQGSNGVSCCQDET--- 304

Query: 304 GKNIIIEENSKEASEDQVPKKASKSCCWAGKWEQSEILAAVAIVGAVATVAVAYSFYRRS 363
                          + + K+  K   W    ++ E+    A+VGA+AT+A+A++F++RS
Sbjct: 305 ------------PKPEPIKKEVKKCTIWFQPLDKEELYIGAAVVGAIATIAMAFTFFKRS 332

BLAST of MS003114 vs. TAIR 10
Match: AT4G26620.1 (Sucrase/ferredoxin-like family protein )

HSP 1 Score: 325.1 bits (832), Expect = 7.2e-89
Identity = 176/387 (45.48%), Postives = 237/387 (61.24%), Query Frame = 0

Query: 13  DDENYGFRRQEMYQSDLAGTVNAYDRHVFLCYESPESWPSNFEKSDSDVLPKLLSAALKD 72
           +D ++GF R +     LAGTV  Y+RHVFLCY+ P  WP+  E ++ D LP+LLSAA+  
Sbjct: 59  NDADFGFARPDFRSEQLAGTVQFYERHVFLCYKKPSVWPARIEAAEFDRLPRLLSAAVSA 118

Query: 73  RKDDISGKTKLTVFAGREETGFSDGDVLIFPEMVKYRGLKESDVDGFVNDVLVNDKPWAS 132
           RK  +  +T+LT+  G + T  S+GDVLIFP+M++YR L   DV+ FV +VLV D  W  
Sbjct: 119 RKGSMKKETRLTICEGHDGTETSNGDVLIFPDMIRYRRLTHFDVETFVEEVLVKDGEWLP 178

Query: 133 GVQEVLTGSHVFVCVHASRDRRCGVCGPILVHKFDEEIELRGLKDQVLVSPCSHVGGHKY 192
           G  E+L GS+VFVC H SRDRRCGVCGP LV +F EE+E  GL+ +V +SPCSH+GGHKY
Sbjct: 179 GNPELLKGSYVFVCSHGSRDRRCGVCGPSLVSRFREELEFHGLQGKVSISPCSHIGGHKY 238

Query: 193 AGNLIIYQHGVDGKTTGHWYGYVTPEDVPGLLEQHIAKGEVVERLLRGQMGASPEEGKKA 252
           AGN+IIY+  ++ + TGHWYGYVTPEDVP LLEQHI KGE+V+RL RG+MG S E+ KK 
Sbjct: 239 AGNVIIYRSNINREVTGHWYGYVTPEDVPILLEQHINKGEIVDRLWRGEMGLSEEDQKKT 298

Query: 253 GEQNLP----------NGEDTKKSKVEIQENGD----QSKVETTAGCC-QGSNGFTCCRD 312
            E              NG+ +++S V    N D    QS+     GCC Q  N  +CC+D
Sbjct: 299 QEGRFQLNGTVHSVKINGKVSQESSV---HNADVSCCQSRAAEPNGCCQQNGNSSSCCQD 358

Query: 313 ETS------GSGKNIIIEENSKE-----------------ASEDQVPKKASKSCCWAGKW 362
           +T+       S  N +  EN+ E                 + +    +K      W   W
Sbjct: 359 DTTLMLSLGTSEDNQLESENNTEKLTPGRKIAEKTFFRINSDKGSSTRKVCGIPTWLESW 418

BLAST of MS003114 vs. TAIR 10
Match: AT5G55900.1 (Sucrase/ferredoxin-like family protein )

HSP 1 Score: 289.3 bits (739), Expect = 4.4e-78
Identity = 157/349 (44.99%), Postives = 208/349 (59.60%), Query Frame = 0

Query: 29  LAGTVNAYDRHVFLCYESPESWPSNFEKSDSDVLPKLLSAALKDRKDDISGKTKLTVFAG 88
           LAGTV  Y+RHVFLCY+ P  WP+  E S+ D LP+LLS+ +  RK  +  +T LT+  G
Sbjct: 63  LAGTVQFYERHVFLCYKKPSVWPARIEASEFDRLPRLLSSVISARKSSMKKETLLTICEG 122

Query: 89  REETGFSDGDVLIFPEMVKYRGLKESDVDGFVNDVLVNDKPWASGVQEVLTGSHVFVCVH 148
            + +  S+GDVLIFP+M++YR L   DVD FV +VLV    W  G  E L+ S+VFVC H
Sbjct: 123 HDGSETSNGDVLIFPDMIRYRRLTHFDVDTFVEEVLVKGVEWLPGNPESLSSSYVFVCCH 182

Query: 149 ASRDRRCGVCGPILVHKFDEEIELRGLKDQVLVSPCSHVGGHKYAGNLIIYQHGVDGKTT 208
            SRDRRCGVCGP LV +F EEI+  GL+ +V VSPCSH+GGHKY G++IIY   ++ + T
Sbjct: 183 GSRDRRCGVCGPSLVSRFREEIDSCGLRGEVSVSPCSHIGGHKYTGDVIIYGLNINQRVT 242

Query: 209 GHWYGYVTPEDVPGLLEQHIAKGEVVERLLRGQMGASPEEGKKAGEQNLPNGEDTKKSKV 268
           GHWYG VT EDVP LLEQHI KGE+V+RL RG+MG   E+ KK  EQ L    +   ++ 
Sbjct: 243 GHWYGCVTLEDVPLLLEQHINKGEIVDRLWRGEMGLPEEDQKKTQEQRLQLNSEKISNRE 302

Query: 269 EIQENGDQS--------KVETTAGCCQGSNGFTCCRD--------ETSGSGKNIIIEENS 328
             QE+ + S             +GC Q  N   C  +        E   S KN  +   S
Sbjct: 303 VTQESVNNSICCQSRAVPELNGSGCQQNGNSSYCLEEIHTEKNTSERVTSVKNASLRIGS 362

Query: 329 KEASEDQVPKKASKSCCWAGKWEQSEILAAVAIVGAVATVAVAYSFYRR 362
            E       K  +    W   WE+ +  AA+A+  A A+VA+AY+ Y++
Sbjct: 363 SENGSSGGFKVCAVMSMWLETWEREDTYAALAVACAAASVAIAYNCYKQ 411

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022134290.11.6e-20799.45uncharacterized protein LOC111006588 [Momordica charantia][more]
KAG7016209.15.1e-16983.20AIM32 [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_038890361.11.5e-16881.49altered inheritance of mitochondria protein 32-like [Benincasa hispida][more]
KAG6578669.13.3e-16882.64hypothetical protein SDJN03_23117, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_022939649.14.3e-16882.64altered inheritance of mitochondria protein 32-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
A5DK491.2e-1636.04Altered inheritance of mitochondria protein 32 OS=Meyerozyma guilliermondii (str... [more]
Q6BPT62.3e-1541.00Altered inheritance of mitochondria protein 32 OS=Debaryomyces hansenii (strain ... [more]
P382819.7e-1432.69Actin patches distal protein 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 /... [more]
C4Y1G78.2e-1338.38Altered inheritance of mitochondria protein 32 OS=Clavispora lusitaniae (strain ... [more]
Q6FLQ55.3e-1226.44Altered inheritance of mitochondria protein 32 OS=Candida glabrata (strain ATCC ... [more]
Match NameE-valueIdentityDescription
A0A6J1BXW97.8e-20899.45uncharacterized protein LOC111006588 OS=Momordica charantia OX=3673 GN=LOC111006... [more]
A0A6J1FHU32.1e-16882.64altered inheritance of mitochondria protein 32-like OS=Cucurbita moschata OX=366... [more]
A0A6J1JRQ94.3e-16680.39altered inheritance of mitochondria protein 32-like OS=Cucurbita maxima OX=3661 ... [more]
A0A6J1JSI14.8e-16581.27altered inheritance of mitochondria protein 32-like OS=Cucurbita maxima OX=3661 ... [more]
A0A1S3C9A54.8e-16580.49altered inheritance of mitochondria protein 32-like OS=Cucumis melo OX=3656 GN=L... [more]
Match NameE-valueIdentityDescription
AT3G27570.12.9e-9849.45Sucrase/ferredoxin-like family protein [more]
AT5G40510.11.4e-9548.06Sucrase/ferredoxin-like family protein [more]
AT4G26620.17.2e-8945.48Sucrase/ferredoxin-like family protein [more]
AT5G55900.14.4e-7844.99Sucrase/ferredoxin-like family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.40.30.10Glutaredoxincoord: 140..241
e-value: 1.0E-24
score: 88.6
coord: 37..137
e-value: 5.8E-5
score: 25.2
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 243..265
NoneNo IPR availablePANTHERPTHR31902:SF12PROTEIN, PUTATIVE-RELATEDcoord: 6..363
IPR009737Thioredoxin-like ferredoxinPFAMPF06999Suc_Fer-likecoord: 29..240
e-value: 9.9E-44
score: 149.5
IPR009737Thioredoxin-like ferredoxinPANTHERPTHR31902ACTIN PATCHES DISTAL PROTEIN 1coord: 6..363
IPR009737Thioredoxin-like ferredoxinCDDcd03062TRX_Fd_Sucrasecoord: 140..242
e-value: 9.61361E-41
score: 136.991
IPR036249Thioredoxin-like superfamilySUPERFAMILY52833Thioredoxin-likecoord: 142..243

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS003114.1MS003114.1mRNA