MS002876 (gene) Bitter gourd (TR) v1

BLAST of MS002876 vs. NCBI nr
Match: XP_022142446.1 (U-box domain-containing protein 35-like isoform X3 [Momordica charantia])

HSP 1 Score: 557.0 bits (1434), Expect = 9.6e-155
Identity = 281/282 (99.65%), Postives = 281/282 (99.65%), Query Frame = 0

Query: 8   MMGGGIYSDDQGSTRKHYFGSENFQFDRSLSENEEGGSSELFEIELGIEVRSNRKQEDDC 67
           MMGGGIYSDDQGSTRKHYFGSENFQFDRSLSENEEGGSSELFEIELGIEVRSNRKQEDDC
Sbjct: 1   MMGGGIYSDDQGSTRKHYFGSENFQFDRSLSENEEGGSSELFEIELGIEVRSNRKQEDDC 60

Query: 68  ECEFEGSWFSFDFRKSNHCVYVGIGDNIDSSMDALKWTLNFAVLPSTVVYLLHIFPEIRF 127
           ECEFEGSWFSFDFRKSNHCVYVGIGDNIDSSMDALKWTLNFAVLPSTVVYLLHIFPEIRF
Sbjct: 61  ECEFEGSWFSFDFRKSNHCVYVGIGDNIDSSMDALKWTLNFAVLPSTVVYLLHIFPEIRF 120

Query: 128 VPSPLGMLPKSQVSPRLVERHMAEERAKRTEFLRKFVDTCSAAEVKVEAVMIESDMVAKA 187
           VPSPLGMLPKSQVSPRLVERHMAEERAKRTEFLRKFVDTCSAAEVKVEAVMIESDMVAKA
Sbjct: 121 VPSPLGMLPKSQVSPRLVERHMAEERAKRTEFLRKFVDTCSAAEVKVEAVMIESDMVAKA 180

Query: 188 ILDLISIFQIRKLILGSTKTRRKWRSKRGALARQVLQNAGEFCDVKIICEGKEATYQMIE 247
           ILDLISIFQIRKLILGSTKTRRKWRSKRGALARQVLQNAGEFCDVKIICEGKEATYQMIE
Sbjct: 181 ILDLISIFQIRKLILGSTKTRRKWRSKRGALARQVLQNAGEFCDVKIICEGKEATYQMIE 240

Query: 248 SSSSSSSNILSPNISDNENFDIKRMQKKYRHNGFFWCRCFGY 290
           SSSSSSSNILSPNISDNENFDIKRMQKKYRHN FFWCRCFGY
Sbjct: 241 SSSSSSSNILSPNISDNENFDIKRMQKKYRHNSFFWCRCFGY 282

BLAST of MS002876 vs. NCBI nr
Match: XP_022142444.1 (uncharacterized protein LOC111012574 isoform X1 [Momordica charantia])

HSP 1 Score: 551.6 bits (1420), Expect = 4.0e-153
Identity = 281/285 (98.60%), Postives = 281/285 (98.60%), Query Frame = 0

Query: 8   MMGGGIYSDDQGSTRKHYFGSENFQFDRSLSENEEGGSSELFEIELGIEVRSNRKQEDDC 67
           MMGGGIYSDDQGSTRKHYFGSENFQFDRSLSENEEGGSSELFEIELGIEVRSNRKQEDDC
Sbjct: 1   MMGGGIYSDDQGSTRKHYFGSENFQFDRSLSENEEGGSSELFEIELGIEVRSNRKQEDDC 60

Query: 68  ECEFEGSWFSFDFRKSNHCVYVGIGDNIDSSMDALKWTLNFAVLPSTVVYLLHIFPEIRF 127
           ECEFEGSWFSFDFRKSNHCVYVGIGDNIDSSMDALKWTLNFAVLPSTVVYLLHIFPEIRF
Sbjct: 61  ECEFEGSWFSFDFRKSNHCVYVGIGDNIDSSMDALKWTLNFAVLPSTVVYLLHIFPEIRF 120

Query: 128 VPSPLGMLPKSQVSPRLVERHMAEERAKRTEFLRKFVDTCSAAE---VKVEAVMIESDMV 187
           VPSPLGMLPKSQVSPRLVERHMAEERAKRTEFLRKFVDTCSAAE   VKVEAVMIESDMV
Sbjct: 121 VPSPLGMLPKSQVSPRLVERHMAEERAKRTEFLRKFVDTCSAAEFEQVKVEAVMIESDMV 180

Query: 188 AKAILDLISIFQIRKLILGSTKTRRKWRSKRGALARQVLQNAGEFCDVKIICEGKEATYQ 247
           AKAILDLISIFQIRKLILGSTKTRRKWRSKRGALARQVLQNAGEFCDVKIICEGKEATYQ
Sbjct: 181 AKAILDLISIFQIRKLILGSTKTRRKWRSKRGALARQVLQNAGEFCDVKIICEGKEATYQ 240

Query: 248 MIESSSSSSSNILSPNISDNENFDIKRMQKKYRHNGFFWCRCFGY 290
           MIESSSSSSSNILSPNISDNENFDIKRMQKKYRHN FFWCRCFGY
Sbjct: 241 MIESSSSSSSNILSPNISDNENFDIKRMQKKYRHNSFFWCRCFGY 285

BLAST of MS002876 vs. NCBI nr
Match: XP_022142447.1 (U-box domain-containing protein 35-like isoform X4 [Momordica charantia])

HSP 1 Score: 550.8 bits (1418), Expect = 6.9e-153
Identity = 280/282 (99.29%), Postives = 280/282 (99.29%), Query Frame = 0

Query: 8   MMGGGIYSDDQGSTRKHYFGSENFQFDRSLSENEEGGSSELFEIELGIEVRSNRKQEDDC 67
           MMGGGIYSDDQGSTRKHYFGSENFQFDRSLSENEEGGSSELFEIELGIEVRSNRKQEDDC
Sbjct: 1   MMGGGIYSDDQGSTRKHYFGSENFQFDRSLSENEEGGSSELFEIELGIEVRSNRKQEDDC 60

Query: 68  ECEFEGSWFSFDFRKSNHCVYVGIGDNIDSSMDALKWTLNFAVLPSTVVYLLHIFPEIRF 127
           ECEFEGSWFSFDFRKSNHCVYVGIGDNIDSSMDALKWTLNFAVLPSTVVYLLHIFPEIRF
Sbjct: 61  ECEFEGSWFSFDFRKSNHCVYVGIGDNIDSSMDALKWTLNFAVLPSTVVYLLHIFPEIRF 120

Query: 128 VPSPLGMLPKSQVSPRLVERHMAEERAKRTEFLRKFVDTCSAAEVKVEAVMIESDMVAKA 187
           VPSPLGMLPKSQVSPRLVERHMAEERAKRTEFLRKFVDTCSAAEVKVEAVMIESDMVAKA
Sbjct: 121 VPSPLGMLPKSQVSPRLVERHMAEERAKRTEFLRKFVDTCSAAEVKVEAVMIESDMVAKA 180

Query: 188 ILDLISIFQIRKLILGSTKTRRKWRSKRGALARQVLQNAGEFCDVKIICEGKEATYQMIE 247
           ILDLISIFQIRKLILGSTKT RKWRSKRGALARQVLQNAGEFCDVKIICEGKEATYQMIE
Sbjct: 181 ILDLISIFQIRKLILGSTKT-RKWRSKRGALARQVLQNAGEFCDVKIICEGKEATYQMIE 240

Query: 248 SSSSSSSNILSPNISDNENFDIKRMQKKYRHNGFFWCRCFGY 290
           SSSSSSSNILSPNISDNENFDIKRMQKKYRHN FFWCRCFGY
Sbjct: 241 SSSSSSSNILSPNISDNENFDIKRMQKKYRHNSFFWCRCFGY 281

BLAST of MS002876 vs. NCBI nr
Match: XP_022142445.1 (uncharacterized protein LOC111012574 isoform X2 [Momordica charantia])

HSP 1 Score: 545.4 bits (1404), Expect = 2.9e-151
Identity = 280/285 (98.25%), Postives = 280/285 (98.25%), Query Frame = 0

Query: 8   MMGGGIYSDDQGSTRKHYFGSENFQFDRSLSENEEGGSSELFEIELGIEVRSNRKQEDDC 67
           MMGGGIYSDDQGSTRKHYFGSENFQFDRSLSENEEGGSSELFEIELGIEVRSNRKQEDDC
Sbjct: 1   MMGGGIYSDDQGSTRKHYFGSENFQFDRSLSENEEGGSSELFEIELGIEVRSNRKQEDDC 60

Query: 68  ECEFEGSWFSFDFRKSNHCVYVGIGDNIDSSMDALKWTLNFAVLPSTVVYLLHIFPEIRF 127
           ECEFEGSWFSFDFRKSNHCVYVGIGDNIDSSMDALKWTLNFAVLPSTVVYLLHIFPEIRF
Sbjct: 61  ECEFEGSWFSFDFRKSNHCVYVGIGDNIDSSMDALKWTLNFAVLPSTVVYLLHIFPEIRF 120

Query: 128 VPSPLGMLPKSQVSPRLVERHMAEERAKRTEFLRKFVDTCSAAE---VKVEAVMIESDMV 187
           VPSPLGMLPKSQVSPRLVERHMAEERAKRTEFLRKFVDTCSAAE   VKVEAVMIESDMV
Sbjct: 121 VPSPLGMLPKSQVSPRLVERHMAEERAKRTEFLRKFVDTCSAAEFEQVKVEAVMIESDMV 180

Query: 188 AKAILDLISIFQIRKLILGSTKTRRKWRSKRGALARQVLQNAGEFCDVKIICEGKEATYQ 247
           AKAILDLISIFQIRKLILGSTKT RKWRSKRGALARQVLQNAGEFCDVKIICEGKEATYQ
Sbjct: 181 AKAILDLISIFQIRKLILGSTKT-RKWRSKRGALARQVLQNAGEFCDVKIICEGKEATYQ 240

Query: 248 MIESSSSSSSNILSPNISDNENFDIKRMQKKYRHNGFFWCRCFGY 290
           MIESSSSSSSNILSPNISDNENFDIKRMQKKYRHN FFWCRCFGY
Sbjct: 241 MIESSSSSSSNILSPNISDNENFDIKRMQKKYRHNSFFWCRCFGY 284

BLAST of MS002876 vs. NCBI nr
Match: XP_038894104.1 (U-box domain-containing protein 35-like [Benincasa hispida])

HSP 1 Score: 360.9 bits (925), Expect = 1.0e-95
Identity = 199/268 (74.25%), Postives = 220/268 (82.09%), Query Frame = 0

Query: 27  GSENFQFDRSLSENEEGGSSELFEIELGIEVRSNRKQEDDCECEFEGSWFSFDFRKSNHC 86
           G ++F  DRSL  NE+  SSELFEIELGIEVRSNRKQEDDC    E SWFSFDFRKSNHC
Sbjct: 6   GGDDFLLDRSLIANEDEESSELFEIELGIEVRSNRKQEDDC----EESWFSFDFRKSNHC 65

Query: 87  VYVGIGDNIDSSMDALKWTLNFAVLPSTVVYLLHIFPEIRFVPSPLGMLPKSQVSPRLVE 146
           VYVGI DN DSSMDAL+WTL+FAVLPST+VYL H+FPE+RFVPSPLGMLPKSQVSP+LVE
Sbjct: 66  VYVGISDNFDSSMDALQWTLDFAVLPSTIVYLFHVFPELRFVPSPLGMLPKSQVSPKLVE 125

Query: 147 RHMAEERAKRTEFLRKFVDTCSAAEVKVEAVMIESDMVAKAILDLISIFQIRKLILGSTK 206
           +  AEERAKR EFL KFVD C AA+V VE VMIESDMVAKAILDLIS+ QIRKLILGSTK
Sbjct: 126 KFKAEERAKRKEFLHKFVDKCYAAQVNVEVVMIESDMVAKAILDLISLLQIRKLILGSTK 185

Query: 207 TRRKWRSKRG-ALARQVLQNAGEFCDVKIICEGKEATYQMIE--SSSSSSSNILSPNISD 266
             RKWRS+RG  +AR VLQNA EFCDVKIICEGKE+TYQ IE  SSSSSSS+  SPN+SD
Sbjct: 186 A-RKWRSRRGKGIARHVLQNAQEFCDVKIICEGKESTYQAIESSSSSSSSSHTPSPNLSD 245

Query: 267 -NENF--DIKRMQKKYRHNGFFWCRCFG 289
            N +F    K  +K+Y  +GF   RCFG
Sbjct: 246 KNIHFKSHFKNTEKEYHRSGFLTWRCFG 268

BLAST of MS002876 vs. ExPASy Swiss-Prot
Match: Q9SW11 (U-box domain-containing protein 35 OS=Arabidopsis thaliana OX=3702 GN=PUB35 PE=2 SV=2)

HSP 1 Score: 51.6 bits (122), Expect = 1.7e-05
Identity = 41/152 (26.97%), Postives = 77/152 (50.66%), Query Frame = 0

Query: 104 WTL-NFAVLPSTVVYLLHIFPEIRFVPSPLG-MLPKSQVSPRLVERHMAEERAKRTEFLR 163
           W +  FA   +    LLHI P I  VP+P+G  +P S+V   +V  +  E   +  E L+
Sbjct: 38  WAIEKFATEGNVGFKLLHIHPMITSVPTPMGNAIPISEVRDDVVTAYRQEILWQSEEMLK 97

Query: 164 KFVDTCSAAEVKVEAVMIESDMVAKAILDLISIFQIRKLILGSTKTRRKWRSKRGALARQ 223
            +       +V VE ++IESD VA AI + ++   I ++++G +   R + S++  +   
Sbjct: 98  PYTKLFVRRKVAVEVLVIESDNVAAAIAEEVTRDSIDRIVIGGSS--RSFFSRKADICSV 157

Query: 224 VLQNAGEFCDVKIICEGKEATYQMIESSSSSS 254
           +      FC V ++ +GK +  +  +S  +++
Sbjct: 158 ISALMPNFCTVYVVSKGKLSCVRPSDSDGNAT 187

BLAST of MS002876 vs. ExPASy Swiss-Prot
Match: Q8GUH1 (U-box domain-containing protein 33 OS=Arabidopsis thaliana OX=3702 GN=PUB33 PE=2 SV=2)

HSP 1 Score: 49.3 bits (116), Expect = 8.6e-05
Identity = 50/202 (24.75%), Postives = 85/202 (42.08%), Query Frame = 0

Query: 87  VYVGIGDNIDSSMDALKWTLNFAVLPSTVVYLLHIFPEIRFVPSPLGMLPKSQVSPRLVE 146
           ++V +  ++  S   L W L         + L+H+    + +P      P   V    V 
Sbjct: 47  IFVAVDKHVAKSKSTLIWALQNT--GGKKICLIHVHQPSQMIPLMGAKFPVGAVKEEEVR 106

Query: 147 RHMAEERAKRTEFLRKFVDTCSAAEVKVEAVMIESDMVAKAILDLISIFQIRKLILGSTK 206
               +ER K    L  ++  C    V+ E + IE + +   I+ LIS   IRKL++G+  
Sbjct: 107 VFREKEREKVHMILDDYLRICQQRGVRAEKMFIEMESIENGIVQLISELGIRKLVMGAAA 166

Query: 207 TR---RKWRSKRGALARQVLQNAGEFCDVKIICEG-----KEATYQMIESS------SSS 266
            R   R+    +   A  V + A   C +   C+G     +EAT    ES       S S
Sbjct: 167 DRHYSRRMTDLKSRKAIFVRREAPTLCQIWFTCKGYLIHTREATMDDTESEYASPRPSIS 226

Query: 267 SSNILSP-NISDNENFDIKRMQ 274
           +S++L   +  ++E+  I R+Q
Sbjct: 227 ASDLLQTFSTPESEHQHISRVQ 246

BLAST of MS002876 vs. ExPASy TrEMBL
Match: A0A6J1CM72 (U-box domain-containing protein 35-like isoform X3 OS=Momordica charantia OX=3673 GN=LOC111012574 PE=4 SV=1)

HSP 1 Score: 557.0 bits (1434), Expect = 4.7e-155
Identity = 281/282 (99.65%), Postives = 281/282 (99.65%), Query Frame = 0

Query: 8   MMGGGIYSDDQGSTRKHYFGSENFQFDRSLSENEEGGSSELFEIELGIEVRSNRKQEDDC 67
           MMGGGIYSDDQGSTRKHYFGSENFQFDRSLSENEEGGSSELFEIELGIEVRSNRKQEDDC
Sbjct: 1   MMGGGIYSDDQGSTRKHYFGSENFQFDRSLSENEEGGSSELFEIELGIEVRSNRKQEDDC 60

Query: 68  ECEFEGSWFSFDFRKSNHCVYVGIGDNIDSSMDALKWTLNFAVLPSTVVYLLHIFPEIRF 127
           ECEFEGSWFSFDFRKSNHCVYVGIGDNIDSSMDALKWTLNFAVLPSTVVYLLHIFPEIRF
Sbjct: 61  ECEFEGSWFSFDFRKSNHCVYVGIGDNIDSSMDALKWTLNFAVLPSTVVYLLHIFPEIRF 120

Query: 128 VPSPLGMLPKSQVSPRLVERHMAEERAKRTEFLRKFVDTCSAAEVKVEAVMIESDMVAKA 187
           VPSPLGMLPKSQVSPRLVERHMAEERAKRTEFLRKFVDTCSAAEVKVEAVMIESDMVAKA
Sbjct: 121 VPSPLGMLPKSQVSPRLVERHMAEERAKRTEFLRKFVDTCSAAEVKVEAVMIESDMVAKA 180

Query: 188 ILDLISIFQIRKLILGSTKTRRKWRSKRGALARQVLQNAGEFCDVKIICEGKEATYQMIE 247
           ILDLISIFQIRKLILGSTKTRRKWRSKRGALARQVLQNAGEFCDVKIICEGKEATYQMIE
Sbjct: 181 ILDLISIFQIRKLILGSTKTRRKWRSKRGALARQVLQNAGEFCDVKIICEGKEATYQMIE 240

Query: 248 SSSSSSSNILSPNISDNENFDIKRMQKKYRHNGFFWCRCFGY 290
           SSSSSSSNILSPNISDNENFDIKRMQKKYRHN FFWCRCFGY
Sbjct: 241 SSSSSSSNILSPNISDNENFDIKRMQKKYRHNSFFWCRCFGY 282

BLAST of MS002876 vs. ExPASy TrEMBL
Match: A0A6J1CLJ4 (uncharacterized protein LOC111012574 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111012574 PE=4 SV=1)

HSP 1 Score: 551.6 bits (1420), Expect = 2.0e-153
Identity = 281/285 (98.60%), Postives = 281/285 (98.60%), Query Frame = 0

Query: 8   MMGGGIYSDDQGSTRKHYFGSENFQFDRSLSENEEGGSSELFEIELGIEVRSNRKQEDDC 67
           MMGGGIYSDDQGSTRKHYFGSENFQFDRSLSENEEGGSSELFEIELGIEVRSNRKQEDDC
Sbjct: 1   MMGGGIYSDDQGSTRKHYFGSENFQFDRSLSENEEGGSSELFEIELGIEVRSNRKQEDDC 60

Query: 68  ECEFEGSWFSFDFRKSNHCVYVGIGDNIDSSMDALKWTLNFAVLPSTVVYLLHIFPEIRF 127
           ECEFEGSWFSFDFRKSNHCVYVGIGDNIDSSMDALKWTLNFAVLPSTVVYLLHIFPEIRF
Sbjct: 61  ECEFEGSWFSFDFRKSNHCVYVGIGDNIDSSMDALKWTLNFAVLPSTVVYLLHIFPEIRF 120

Query: 128 VPSPLGMLPKSQVSPRLVERHMAEERAKRTEFLRKFVDTCSAAE---VKVEAVMIESDMV 187
           VPSPLGMLPKSQVSPRLVERHMAEERAKRTEFLRKFVDTCSAAE   VKVEAVMIESDMV
Sbjct: 121 VPSPLGMLPKSQVSPRLVERHMAEERAKRTEFLRKFVDTCSAAEFEQVKVEAVMIESDMV 180

Query: 188 AKAILDLISIFQIRKLILGSTKTRRKWRSKRGALARQVLQNAGEFCDVKIICEGKEATYQ 247
           AKAILDLISIFQIRKLILGSTKTRRKWRSKRGALARQVLQNAGEFCDVKIICEGKEATYQ
Sbjct: 181 AKAILDLISIFQIRKLILGSTKTRRKWRSKRGALARQVLQNAGEFCDVKIICEGKEATYQ 240

Query: 248 MIESSSSSSSNILSPNISDNENFDIKRMQKKYRHNGFFWCRCFGY 290
           MIESSSSSSSNILSPNISDNENFDIKRMQKKYRHN FFWCRCFGY
Sbjct: 241 MIESSSSSSSNILSPNISDNENFDIKRMQKKYRHNSFFWCRCFGY 285

BLAST of MS002876 vs. ExPASy TrEMBL
Match: A0A6J1CMT0 (U-box domain-containing protein 35-like isoform X4 OS=Momordica charantia OX=3673 GN=LOC111012574 PE=4 SV=1)

HSP 1 Score: 550.8 bits (1418), Expect = 3.3e-153
Identity = 280/282 (99.29%), Postives = 280/282 (99.29%), Query Frame = 0

Query: 8   MMGGGIYSDDQGSTRKHYFGSENFQFDRSLSENEEGGSSELFEIELGIEVRSNRKQEDDC 67
           MMGGGIYSDDQGSTRKHYFGSENFQFDRSLSENEEGGSSELFEIELGIEVRSNRKQEDDC
Sbjct: 1   MMGGGIYSDDQGSTRKHYFGSENFQFDRSLSENEEGGSSELFEIELGIEVRSNRKQEDDC 60

Query: 68  ECEFEGSWFSFDFRKSNHCVYVGIGDNIDSSMDALKWTLNFAVLPSTVVYLLHIFPEIRF 127
           ECEFEGSWFSFDFRKSNHCVYVGIGDNIDSSMDALKWTLNFAVLPSTVVYLLHIFPEIRF
Sbjct: 61  ECEFEGSWFSFDFRKSNHCVYVGIGDNIDSSMDALKWTLNFAVLPSTVVYLLHIFPEIRF 120

Query: 128 VPSPLGMLPKSQVSPRLVERHMAEERAKRTEFLRKFVDTCSAAEVKVEAVMIESDMVAKA 187
           VPSPLGMLPKSQVSPRLVERHMAEERAKRTEFLRKFVDTCSAAEVKVEAVMIESDMVAKA
Sbjct: 121 VPSPLGMLPKSQVSPRLVERHMAEERAKRTEFLRKFVDTCSAAEVKVEAVMIESDMVAKA 180

Query: 188 ILDLISIFQIRKLILGSTKTRRKWRSKRGALARQVLQNAGEFCDVKIICEGKEATYQMIE 247
           ILDLISIFQIRKLILGSTKT RKWRSKRGALARQVLQNAGEFCDVKIICEGKEATYQMIE
Sbjct: 181 ILDLISIFQIRKLILGSTKT-RKWRSKRGALARQVLQNAGEFCDVKIICEGKEATYQMIE 240

Query: 248 SSSSSSSNILSPNISDNENFDIKRMQKKYRHNGFFWCRCFGY 290
           SSSSSSSNILSPNISDNENFDIKRMQKKYRHN FFWCRCFGY
Sbjct: 241 SSSSSSSNILSPNISDNENFDIKRMQKKYRHNSFFWCRCFGY 281

BLAST of MS002876 vs. ExPASy TrEMBL
Match: A0A6J1CKY9 (uncharacterized protein LOC111012574 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111012574 PE=4 SV=1)

HSP 1 Score: 545.4 bits (1404), Expect = 1.4e-151
Identity = 280/285 (98.25%), Postives = 280/285 (98.25%), Query Frame = 0

Query: 8   MMGGGIYSDDQGSTRKHYFGSENFQFDRSLSENEEGGSSELFEIELGIEVRSNRKQEDDC 67
           MMGGGIYSDDQGSTRKHYFGSENFQFDRSLSENEEGGSSELFEIELGIEVRSNRKQEDDC
Sbjct: 1   MMGGGIYSDDQGSTRKHYFGSENFQFDRSLSENEEGGSSELFEIELGIEVRSNRKQEDDC 60

Query: 68  ECEFEGSWFSFDFRKSNHCVYVGIGDNIDSSMDALKWTLNFAVLPSTVVYLLHIFPEIRF 127
           ECEFEGSWFSFDFRKSNHCVYVGIGDNIDSSMDALKWTLNFAVLPSTVVYLLHIFPEIRF
Sbjct: 61  ECEFEGSWFSFDFRKSNHCVYVGIGDNIDSSMDALKWTLNFAVLPSTVVYLLHIFPEIRF 120

Query: 128 VPSPLGMLPKSQVSPRLVERHMAEERAKRTEFLRKFVDTCSAAE---VKVEAVMIESDMV 187
           VPSPLGMLPKSQVSPRLVERHMAEERAKRTEFLRKFVDTCSAAE   VKVEAVMIESDMV
Sbjct: 121 VPSPLGMLPKSQVSPRLVERHMAEERAKRTEFLRKFVDTCSAAEFEQVKVEAVMIESDMV 180

Query: 188 AKAILDLISIFQIRKLILGSTKTRRKWRSKRGALARQVLQNAGEFCDVKIICEGKEATYQ 247
           AKAILDLISIFQIRKLILGSTKT RKWRSKRGALARQVLQNAGEFCDVKIICEGKEATYQ
Sbjct: 181 AKAILDLISIFQIRKLILGSTKT-RKWRSKRGALARQVLQNAGEFCDVKIICEGKEATYQ 240

Query: 248 MIESSSSSSSNILSPNISDNENFDIKRMQKKYRHNGFFWCRCFGY 290
           MIESSSSSSSNILSPNISDNENFDIKRMQKKYRHN FFWCRCFGY
Sbjct: 241 MIESSSSSSSNILSPNISDNENFDIKRMQKKYRHNSFFWCRCFGY 284

BLAST of MS002876 vs. ExPASy TrEMBL
Match: A0A6J1IAG5 (U-box domain-containing protein 35-like OS=Cucurbita maxima OX=3661 GN=LOC111471613 PE=4 SV=1)

HSP 1 Score: 358.6 bits (919), Expect = 2.4e-95
Identity = 202/289 (69.90%), Postives = 237/289 (82.01%), Query Frame = 0

Query: 5   ESNMMGGGIYSDDQGSTRKHYF-GSENFQFDRSLSENEEGGSSELFEIELGIEVRSNR-K 64
           ES ++ GG+ SD Q ST KHY+ GS++F+F RSLS NEE  SS+LFEI+LG+ V+SNR K
Sbjct: 5   ESLLVEGGVRSDVQESTSKHYYLGSDDFKFHRSLSVNEEEKSSDLFEIDLGLHVQSNRNK 64

Query: 65  QEDDCECEFEGSWFSFDFRKSNHCVYVGIGDNIDSSMDALKWTLNFAVLPSTVVYLLHIF 124
            EDDC    E SWFSFDFRKSNHCVYVGIGDN  SSM AL+WTL+FAVLPS++VYLLH+ 
Sbjct: 65  LEDDC----EESWFSFDFRKSNHCVYVGIGDNDASSMAALQWTLDFAVLPSSIVYLLHVS 124

Query: 125 PEIRFVPSPLGMLPKSQVSPRLVERHMAEERAKRTEFLRKFVDTCSAAEVKVEAVMIESD 184
           PEIRFVPSPLGMLP+SQV P++VE  MAEERAKR EFL KFVDTCS+A+V VE VMIESD
Sbjct: 125 PEIRFVPSPLGMLPRSQVGPKIVETFMAEERAKRREFLHKFVDTCSSAQVNVEVVMIESD 184

Query: 185 MVAKAILDLISIFQIRKLILG--STKTRRKWRSKRGALARQVLQNAGEFCDVKIIC-EGK 244
           MVAKAILDLIS+ QIRKLILG  STK RRKWRSKR  +A+Q+L+NA EFCDVKIIC EGK
Sbjct: 185 MVAKAILDLISLLQIRKLILGRNSTKARRKWRSKREGIAKQILKNAEEFCDVKIICEEGK 244

Query: 245 EATYQMIESSSSSSSNILSPNISDNENFDIKRMQKKYRHNGFFWCRCFG 289
           E+T Q+I+SSSSSSS+    + S + NF I  M+K+YRH+GF   RCFG
Sbjct: 245 ESTCQVIDSSSSSSSS----SSSCSTNFKI--MKKEYRHSGFVSWRCFG 283

BLAST of MS002876 vs. TAIR 10
Match: AT5G47740.1 (Adenine nucleotide alpha hydrolases-like superfamily protein )

HSP 1 Score: 142.1 bits (357), Expect = 6.9e-34
Identity = 92/233 (39.48%), Postives = 134/233 (57.51%), Query Frame = 0

Query: 61  RKQEDDCECEFEGSWFSFDFRKSNHCVYVGIGDNIDSSMDALKWTL-NFAVLPSTVVYLL 120
           R+ E   E + EG             VYVG+G   DSSM+AL+W + N     ST+++L+
Sbjct: 19  RRLESVRESQLEGRSSGTVSMNGEDNVYVGVGKG-DSSMEALRWAIDNLMTSSSTLLFLI 78

Query: 121 HIFPEIRFVPSPLGMLPKSQVSPRLVERHMAEERAKRTEFLRKFVDTCSAAEVKVEAVMI 180
           H+FPE RF+P PLG + + + S   VE  M++ER KR   L KF+  CSA++VKVE +++
Sbjct: 79  HVFPETRFIPYPLGRITRERASQEQVESFMSQEREKRRTLLLKFLHACSASKVKVETILV 138

Query: 181 ESDMVAKAILDLISIFQIRKLILGSTKTR-RKWRSKRGALARQVL--QNAGEFCDVKIIC 240
           ESD VAKA+ DLI+I  IRKL+LG  K+  RK  + +G    +++   +A + C+VK+IC
Sbjct: 139 ESDSVAKAVQDLITILNIRKLVLGIDKSNARKASTMKGNSVPELIMRSSAADVCEVKVIC 198

Query: 241 EGKEATYQ--MIESSSSSSSNILSPNISDNENFDIKRMQKKYRHNGFFWCRCF 288
           +GKE   +  M+ESS + S               I + QKK +    F C CF
Sbjct: 199 QGKEINMEQTMMESSPAKS--------------PIVQRQKKDQPVDPFACICF 236

BLAST of MS002876 vs. TAIR 10
Match: AT5G47740.2 (Adenine nucleotide alpha hydrolases-like superfamily protein )

HSP 1 Score: 137.5 bits (345), Expect = 1.7e-32
Identity = 92/235 (39.15%), Postives = 134/235 (57.02%), Query Frame = 0

Query: 61  RKQEDDCECEFEGSWFSFDFRKSNHCVYVGIGDNIDSSMDALKWTL-NFAVLPSTVVYLL 120
           R+ E   E + EG             VYVG+G   DSSM+AL+W + N     ST+++L+
Sbjct: 19  RRLESVRESQLEGRSSGTVSMNGEDNVYVGVGKG-DSSMEALRWAIDNLMTSSSTLLFLI 78

Query: 121 HIFPEIRFVPSPLGMLPKSQVSPRLVERHMAEERAKRTEFLRKFVDTCSAA--EVKVEAV 180
           H+FPE RF+P PLG + + + S   VE  M++ER KR   L KF+  CSA+  +VKVE +
Sbjct: 79  HVFPETRFIPYPLGRITRERASQEQVESFMSQEREKRRTLLLKFLHACSASKEQVKVETI 138

Query: 181 MIESDMVAKAILDLISIFQIRKLILGSTKTR-RKWRSKRGALARQVL--QNAGEFCDVKI 240
           ++ESD VAKA+ DLI+I  IRKL+LG  K+  RK  + +G    +++   +A + C+VK+
Sbjct: 139 LVESDSVAKAVQDLITILNIRKLVLGIDKSNARKASTMKGNSVPELIMRSSAADVCEVKV 198

Query: 241 ICEGKEATYQ--MIESSSSSSSNILSPNISDNENFDIKRMQKKYRHNGFFWCRCF 288
           IC+GKE   +  M+ESS + S               I + QKK +    F C CF
Sbjct: 199 ICQGKEINMEQTMMESSPAKS--------------PIVQRQKKDQPVDPFACICF 238

BLAST of MS002876 vs. TAIR 10
Match: AT5G65500.1 (U-box domain-containing protein kinase family protein )

HSP 1 Score: 69.7 bits (169), Expect = 4.3e-12
Identity = 55/177 (31.07%), Postives = 91/177 (51.41%), Query Frame = 0

Query: 87  VYVGIGDNIDSSMDALKWTL-NFAVLPSTVVYLLHIFPEIR-FVPSPLGMLPKSQVSPRL 146
           VY+ +G+++      + W L  +  +P ++V LLH+    + FV +P G LP S VS   
Sbjct: 22  VYIAVGNDVQEGYKTIHWALKKWNNIPISIV-LLHLCNISQDFVYTPFGKLPASSVSEEK 81

Query: 147 VERHMAEERAKRTEFLRKFVDTCSAAEVKVEAVMIESDMVAKAILDLISIFQIRKLILGS 206
           ++     E  K  + L K++  C   + ++  V  + D +   ILDLIS  +I KL++G 
Sbjct: 82  LQVLRKYEDQKIDKLLSKYITFCGKVKAELLKVEKQHDSIQVLILDLISKLRITKLVMGI 141

Query: 207 T--KTRRKWRSKRGALAR-QVLQNAGEFCDVKIICEGKEATYQMIESSSSSSSNILS 259
           T  ++   W+SK        V QN  EFC+  IIC GK  + +     ++++SNI S
Sbjct: 142 TFMRSSSSWKSKSAISGSFYVYQNKPEFCEFYIICGGKMVSLK--NDVNNNNSNIRS 195

BLAST of MS002876 vs. TAIR 10
Match: AT5G57035.1 (U-box domain-containing protein kinase family protein )

HSP 1 Score: 59.3 bits (142), Expect = 5.9e-09
Identity = 48/189 (25.40%), Postives = 91/189 (48.15%), Query Frame = 0

Query: 91  IGDNI--DSSMDALKWTLNFAVLPSTVVYLLHIFPEIRFVPSPLG-MLPKSQVSPRLVER 150
           +GD +   +S  AL+WT+   +     + L+H+ P +  +PSP G  +P  ++   +V  
Sbjct: 29  VGDAVGGTASRRALRWTIENFLPKIDRLVLVHVMPTVTTIPSPSGSKIPIEELDESVVSM 88

Query: 151 HMAEERAKRTEFLRKFVDTCSAAEVKVEAVMIESDMVAKAILDLISIFQIRKLILGSTKT 210
           +  + R +  +    F   C +   KVE +++E    AKA+L  +S   +  L++GS  +
Sbjct: 89  YKRDLRKEFEQVFVPFKRICKSN--KVETLLLEHHDPAKALLKYMSDTDVECLVIGSCSS 148

Query: 211 RRKWRSKRGALARQVLQNAGEFCDVKIICEGKEATYQMIESSSSSSSNILSPNISD--NE 270
               R K   +   VL  A E C++ ++C+ +  T    + ++ SSS+   P  ++   E
Sbjct: 149 NFLTRKKGQEMPLTVLGEAPETCEIYVVCKDRILTKSTNQFTADSSSSFRIPEGAEAYTE 208

Query: 271 NFDIKRMQK 275
           +F   R  K
Sbjct: 209 SFSRTRSDK 215

BLAST of MS002876 vs. TAIR 10
Match: AT1G16760.1 (Protein kinase protein with adenine nucleotide alpha hydrolases-like domain )

HSP 1 Score: 53.9 bits (128), Expect = 2.5e-07
Identity = 48/184 (26.09%), Postives = 82/184 (44.57%), Query Frame = 0

Query: 93  DNIDSSMDALKWTLNFAVLPSTVVYLLHIFPEIRFVPSPLGMLPKSQVSPRLVE--RHMA 152
           D   SS +A+KWTL         + L+H+             LPKSQ S  + E   H  
Sbjct: 23  DKDKSSQNAIKWTLENLATRGQTLALIHV-------------LPKSQSSLDIEEGITHKQ 82

Query: 153 EERAKRTEFLRKFVDTCSAAEVKVEAVMIESDMVAKAILDLISIFQIRKLILGSTKTRRK 212
           +   +  +    F   CS  E+    V++E     KAI++ +++  I  L+LG+      
Sbjct: 83  QMEKQTKDLFVSFHCYCSRKEIHCLDVVLEDVDKVKAIVEYVTVSAIENLVLGAPSRNSF 142

Query: 213 WRSKRGALARQVLQNAGEFCDVKIICEGKEATYQMIESSSSSSSNILSPNISDNENFDIK 272
            R  +  L   V + A +FC+V +I +GK ++ +     +    ++LS  + D+E   I+
Sbjct: 143 MRRFKTDLPTSVSKAAPDFCNVYVISKGKISSLRNASRLAPYHPSVLS-EVDDHETIAIE 192

Query: 273 RMQK 275
           R  K
Sbjct: 203 RKHK 192