MS002863 (gene) Bitter gourd (TR) v1

Overview
NameMS002863
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionPentatricopeptide repeat-containing protein
Locationscaffold359: 281618 .. 283009 (+)
RNA-Seq ExpressionMS002863
SyntenyMS002863
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATCGTTCTATGGACCTCTTCCATCGCTCGCTACTGCCGCAATGGCCAATTAGCCGAAGCGGCCGCAGAGTTTACAAGCATGAGACTCGCCAGAGTTGAGCCGAACCACGTCACGTTGATTACGCTTCTCTCCGGCTGTGCTGATTTTCCGTCAGAAAGCCTCTACTTCGGCTCTTCGCTCCATGGCTACGCCCGTAAATTAGGTTTGGATACAGCGCATGTAATGGTGGGGACTTCTATCGTTGATATGTATGCCAAATGTGCTCAACTGGGTCTTGCTAGGAGGGTTTTCGATTACCTGAGGATGAAAAACTCTGTTTCTTGGAACACGATGCTCGATGGTTACACGAGGAACGGGGAGATTGAGCTGGCGATTGACCTGTTTGATGAAATGCCTACGAGAGATGCGATTTCTTGGACGGCTTTGATTAATGGTCTCTTGAAACAGGGGTACTCTGAACAAGCATTGGAGTGCTTCCATCAGATGCAGTGCTCAGGTATCAAACCTGATTATGTGTCTATAATTGCCGTTCTTGCTGCGTGTGCTGATTTGGGAACGCTTACTTTGGGGTTATGGGTTAATCGGTTCGTTATGCAGCAGGAGTTTAAGGATAATATTAGGATTAGTAATTCCTTGATAGATATGTATTCTCGATGTGGGTGCATCGGATTTGCCCGCCAAGTGTTTGAGAGAATGTCTAAGCGAACTTTGGTGTCTTGGAACTCCATTATTGTGGGGTATGCTGCTAATGGGTTTGCAGATGAATCTCTGGAGTTTTTTGATGCAATGCAGAAGGAGGGATTCAAGCCAGATGCAGTTAGCTATACGGGAGCTCTTACTGCGTGTAGTCATGCTGGCTTAGTGAATAAGGGGTTGGAATTGTTTGATAACATGAAGAGGGTGCACAGAATTATTCCCAGGATCGAGCATTATGGATGCATTGTCGACCTCTATGGCCGTGCAGGGAGGTTGGAGGATGCGTTGGATGTGATCGAGAAAATGCCGATGAAACCGAATGAAGTTGTGCTTGGATCGTTGCTGGCTGCCTGCAGGACTCATGGCGATGTGAGCTTGGCTGAAAGGTTGATGAAACATCTCTCTAAGTTGGACCCGGGAGGCGATTCAAATTATGTGCTCCTTTCGAACATTTATGCAGCAATTGGAAAGTGGGAAGGGGCTAACAAGGTCAGGAGAACGATGAAAGCCCGAGGTGTGCAGAAAAAACCGGGGTTTAGTTCCGTTGAGATTGATGGTAAGGTTCATGAGTTTGTTGCTGGTGACAAATACCATGCTGATGCAGACAGTATTTATTCTATGTTAGAGCTGCTTGTTCATGAACTAAAGATATGTGGCTCTGTCCCTGAAACTGAAACCTTTCTGAATACCAAAGAA

mRNA sequence

ATCGTTCTATGGACCTCTTCCATCGCTCGCTACTGCCGCAATGGCCAATTAGCCGAAGCGGCCGCAGAGTTTACAAGCATGAGACTCGCCAGAGTTGAGCCGAACCACGTCACGTTGATTACGCTTCTCTCCGGCTGTGCTGATTTTCCGTCAGAAAGCCTCTACTTCGGCTCTTCGCTCCATGGCTACGCCCGTAAATTAGGTTTGGATACAGCGCATGTAATGGTGGGGACTTCTATCGTTGATATGTATGCCAAATGTGCTCAACTGGGTCTTGCTAGGAGGGTTTTCGATTACCTGAGGATGAAAAACTCTGTTTCTTGGAACACGATGCTCGATGGTTACACGAGGAACGGGGAGATTGAGCTGGCGATTGACCTGTTTGATGAAATGCCTACGAGAGATGCGATTTCTTGGACGGCTTTGATTAATGGTCTCTTGAAACAGGGGTACTCTGAACAAGCATTGGAGTGCTTCCATCAGATGCAGTGCTCAGGTATCAAACCTGATTATGTGTCTATAATTGCCGTTCTTGCTGCGTGTGCTGATTTGGGAACGCTTACTTTGGGGTTATGGGTTAATCGGTTCGTTATGCAGCAGGAGTTTAAGGATAATATTAGGATTAGTAATTCCTTGATAGATATGTATTCTCGATGTGGGTGCATCGGATTTGCCCGCCAAGTGTTTGAGAGAATGTCTAAGCGAACTTTGGTGTCTTGGAACTCCATTATTGTGGGGTATGCTGCTAATGGGTTTGCAGATGAATCTCTGGAGTTTTTTGATGCAATGCAGAAGGAGGGATTCAAGCCAGATGCAGTTAGCTATACGGGAGCTCTTACTGCGTGTAGTCATGCTGGCTTAGTGAATAAGGGGTTGGAATTGTTTGATAACATGAAGAGGGTGCACAGAATTATTCCCAGGATCGAGCATTATGGATGCATTGTCGACCTCTATGGCCGTGCAGGGAGGTTGGAGGATGCGTTGGATGTGATCGAGAAAATGCCGATGAAACCGAATGAAGTTGTGCTTGGATCGTTGCTGGCTGCCTGCAGGACTCATGGCGATGTGAGCTTGGCTGAAAGGTTGATGAAACATCTCTCTAAGTTGGACCCGGGAGGCGATTCAAATTATGTGCTCCTTTCGAACATTTATGCAGCAATTGGAAAGTGGGAAGGGGCTAACAAGGTCAGGAGAACGATGAAAGCCCGAGGTGTGCAGAAAAAACCGGGGTTTAGTTCCGTTGAGATTGATGGTAAGGTTCATGAGTTTGTTGCTGGTGACAAATACCATGCTGATGCAGACAGTATTTATTCTATGTTAGAGCTGCTTGTTCATGAACTAAAGATATGTGGCTCTGTCCCTGAAACTGAAACCTTTCTGAATACCAAAGAA

Coding sequence (CDS)

ATCGTTCTATGGACCTCTTCCATCGCTCGCTACTGCCGCAATGGCCAATTAGCCGAAGCGGCCGCAGAGTTTACAAGCATGAGACTCGCCAGAGTTGAGCCGAACCACGTCACGTTGATTACGCTTCTCTCCGGCTGTGCTGATTTTCCGTCAGAAAGCCTCTACTTCGGCTCTTCGCTCCATGGCTACGCCCGTAAATTAGGTTTGGATACAGCGCATGTAATGGTGGGGACTTCTATCGTTGATATGTATGCCAAATGTGCTCAACTGGGTCTTGCTAGGAGGGTTTTCGATTACCTGAGGATGAAAAACTCTGTTTCTTGGAACACGATGCTCGATGGTTACACGAGGAACGGGGAGATTGAGCTGGCGATTGACCTGTTTGATGAAATGCCTACGAGAGATGCGATTTCTTGGACGGCTTTGATTAATGGTCTCTTGAAACAGGGGTACTCTGAACAAGCATTGGAGTGCTTCCATCAGATGCAGTGCTCAGGTATCAAACCTGATTATGTGTCTATAATTGCCGTTCTTGCTGCGTGTGCTGATTTGGGAACGCTTACTTTGGGGTTATGGGTTAATCGGTTCGTTATGCAGCAGGAGTTTAAGGATAATATTAGGATTAGTAATTCCTTGATAGATATGTATTCTCGATGTGGGTGCATCGGATTTGCCCGCCAAGTGTTTGAGAGAATGTCTAAGCGAACTTTGGTGTCTTGGAACTCCATTATTGTGGGGTATGCTGCTAATGGGTTTGCAGATGAATCTCTGGAGTTTTTTGATGCAATGCAGAAGGAGGGATTCAAGCCAGATGCAGTTAGCTATACGGGAGCTCTTACTGCGTGTAGTCATGCTGGCTTAGTGAATAAGGGGTTGGAATTGTTTGATAACATGAAGAGGGTGCACAGAATTATTCCCAGGATCGAGCATTATGGATGCATTGTCGACCTCTATGGCCGTGCAGGGAGGTTGGAGGATGCGTTGGATGTGATCGAGAAAATGCCGATGAAACCGAATGAAGTTGTGCTTGGATCGTTGCTGGCTGCCTGCAGGACTCATGGCGATGTGAGCTTGGCTGAAAGGTTGATGAAACATCTCTCTAAGTTGGACCCGGGAGGCGATTCAAATTATGTGCTCCTTTCGAACATTTATGCAGCAATTGGAAAGTGGGAAGGGGCTAACAAGGTCAGGAGAACGATGAAAGCCCGAGGTGTGCAGAAAAAACCGGGGTTTAGTTCCGTTGAGATTGATGGTAAGGTTCATGAGTTTGTTGCTGGTGACAAATACCATGCTGATGCAGACAGTATTTATTCTATGTTAGAGCTGCTTGTTCATGAACTAAAGATATGTGGCTCTGTCCCTGAAACTGAAACCTTTCTGAATACCAAAGAA

Protein sequence

IVLWTSSIARYCRNGQLAEAAAEFTSMRLARVEPNHVTLITLLSGCADFPSESLYFGSSLHGYARKLGLDTAHVMVGTSIVDMYAKCAQLGLARRVFDYLRMKNSVSWNTMLDGYTRNGEIELAIDLFDEMPTRDAISWTALINGLLKQGYSEQALECFHQMQCSGIKPDYVSIIAVLAACADLGTLTLGLWVNRFVMQQEFKDNIRISNSLIDMYSRCGCIGFARQVFERMSKRTLVSWNSIIVGYAANGFADESLEFFDAMQKEGFKPDAVSYTGALTACSHAGLVNKGLELFDNMKRVHRIIPRIEHYGCIVDLYGRAGRLEDALDVIEKMPMKPNEVVLGSLLAACRTHGDVSLAERLMKHLSKLDPGGDSNYVLLSNIYAAIGKWEGANKVRRTMKARGVQKKPGFSSVEIDGKVHEFVAGDKYHADADSIYSMLELLVHELKICGSVPETETFLNTKE
Homology
BLAST of MS002863 vs. NCBI nr
Match: XP_022142716.1 (pentatricopeptide repeat-containing protein At1g05750, chloroplastic [Momordica charantia])

HSP 1 Score: 941.0 bits (2431), Expect = 3.8e-270
Identity = 462/464 (99.57%), Postives = 463/464 (99.78%), Query Frame = 0

Query: 1   IVLWTSSIARYCRNGQLAEAAAEFTSMRLARVEPNHVTLITLLSGCADFPSESLYFGSSL 60
           IVLWTSSIARYCRNGQLAEAAAEFTSMRLA VEPNHVTLITLLSGCADFPSESLYFGSSL
Sbjct: 57  IVLWTSSIARYCRNGQLAEAAAEFTSMRLAGVEPNHVTLITLLSGCADFPSESLYFGSSL 116

Query: 61  HGYARKLGLDTAHVMVGTSIVDMYAKCAQLGLARRVFDYLRMKNSVSWNTMLDGYTRNGE 120
           HGYARKLGLDTAHVMVGTSIVDMYAKCAQLGLARRVFDYLRMKNSVSWNTMLDGYTRNGE
Sbjct: 117 HGYARKLGLDTAHVMVGTSIVDMYAKCAQLGLARRVFDYLRMKNSVSWNTMLDGYTRNGE 176

Query: 121 IELAIDLFDEMPTRDAISWTALINGLLKQGYSEQALECFHQMQCSGIKPDYVSIIAVLAA 180
           IELAIDLFDEMPTRDAISWTALINGLLKQGYSEQALECFHQMQCSGIKPDYVSIIAVLAA
Sbjct: 177 IELAIDLFDEMPTRDAISWTALINGLLKQGYSEQALECFHQMQCSGIKPDYVSIIAVLAA 236

Query: 181 CADLGTLTLGLWVNRFVMQQEFKDNIRISNSLIDMYSRCGCIGFARQVFERMSKRTLVSW 240
           CADLGTLTLGLWVNRFVMQQEFKDNIRISNSLIDMYSRCGCIGFARQVFERMSKRTLVSW
Sbjct: 237 CADLGTLTLGLWVNRFVMQQEFKDNIRISNSLIDMYSRCGCIGFARQVFERMSKRTLVSW 296

Query: 241 NSIIVGYAANGFADESLEFFDAMQKEGFKPDAVSYTGALTACSHAGLVNKGLELFDNMKR 300
           NSIIVGYAANGFADESLEFFDAMQKEGFKPDAVSYTGALTACSHAGLVNKGLELFDNMKR
Sbjct: 297 NSIIVGYAANGFADESLEFFDAMQKEGFKPDAVSYTGALTACSHAGLVNKGLELFDNMKR 356

Query: 301 VHRIIPRIEHYGCIVDLYGRAGRLEDALDVIEKMPMKPNEVVLGSLLAACRTHGDVSLAE 360
           VHRIIPRIEHYGCIVDLYGRAGRLEDALDVIEKMPMKPNEVVLGSLLAACRTHGDVSLAE
Sbjct: 357 VHRIIPRIEHYGCIVDLYGRAGRLEDALDVIEKMPMKPNEVVLGSLLAACRTHGDVSLAE 416

Query: 361 RLMKHLSKLDPGGDSNYVLLSNIYAAIGKWEGANKVRRTMKARGVQKKPGFSSVEIDGKV 420
           RLMKHLSKLDPGGDSNYVLLSNIYAAIGKWEGANKVRRTMKARGVQKKPGFSSVEIDGKV
Sbjct: 417 RLMKHLSKLDPGGDSNYVLLSNIYAAIGKWEGANKVRRTMKARGVQKKPGFSSVEIDGKV 476

Query: 421 HEFVAGDKYHADADSIYSMLELLVHELKICGSVPETETFLNTKE 465
           HEFVAGDKYHADADSIYSML+LLVHELKICGSVPETETFLNTKE
Sbjct: 477 HEFVAGDKYHADADSIYSMLQLLVHELKICGSVPETETFLNTKE 520

BLAST of MS002863 vs. NCBI nr
Match: XP_038877228.1 (pentatricopeptide repeat-containing protein At1g05750, chloroplastic [Benincasa hispida])

HSP 1 Score: 865.1 bits (2234), Expect = 2.7e-247
Identity = 419/464 (90.30%), Postives = 440/464 (94.83%), Query Frame = 0

Query: 1   IVLWTSSIARYCRNGQLAEAAAEFTSMRLARVEPNHVTLITLLSGCADFPSESLYFGSSL 60
           IVLWTSS+ARYCRNGQL+EAA EFT MRLA VEPNHVT ITLLSGC DFPSESL+FGSSL
Sbjct: 57  IVLWTSSLARYCRNGQLSEAATEFTRMRLAGVEPNHVTFITLLSGCTDFPSESLFFGSSL 116

Query: 61  HGYARKLGLDTAHVMVGTSIVDMYAKCAQLGLARRVFDYLRMKNSVSWNTMLDGYTRNGE 120
           HGYARKLGLDT HVMVGT++VDMYAKCAQ  LAR+VFDYL MKNSV+WNTMLDGYTRNGE
Sbjct: 117 HGYARKLGLDTGHVMVGTALVDMYAKCAQSRLARKVFDYLGMKNSVTWNTMLDGYTRNGE 176

Query: 121 IELAIDLFDEMPTRDAISWTALINGLLKQGYSEQALECFHQMQCSGIKPDYVSIIAVLAA 180
           IELAIDLFDEMPTRDAISWTALINGLLKQG+SEQALECFHQMQCSGI+PDYVSIIAVLAA
Sbjct: 177 IELAIDLFDEMPTRDAISWTALINGLLKQGFSEQALECFHQMQCSGIEPDYVSIIAVLAA 236

Query: 181 CADLGTLTLGLWVNRFVMQQEFKDNIRISNSLIDMYSRCGCIGFARQVFERMSKRTLVSW 240
           CADLG LTLGLWVNRFVMQQEFKDNIRISNSL+DMYSRCGCI FARQVFE+M KRTLVSW
Sbjct: 237 CADLGALTLGLWVNRFVMQQEFKDNIRISNSLVDMYSRCGCIEFARQVFEKMPKRTLVSW 296

Query: 241 NSIIVGYAANGFADESLEFFDAMQKEGFKPDAVSYTGALTACSHAGLVNKGLELFDNMKR 300
           NSIIVG+A NGFADESLEFFDAMQ EGFKPD VSYTGALTACSHAGLVNKGLELFDNMKR
Sbjct: 297 NSIIVGFAVNGFADESLEFFDAMQNEGFKPDGVSYTGALTACSHAGLVNKGLELFDNMKR 356

Query: 301 VHRIIPRIEHYGCIVDLYGRAGRLEDALDVIEKMPMKPNEVVLGSLLAACRTHGDVSLAE 360
           +H+I PRIEHYGCIVDLYGRAGRLEDAL+VIE+MPMKPNEVVLGSLLAACRT+GDVSLAE
Sbjct: 357 IHKITPRIEHYGCIVDLYGRAGRLEDALNVIEEMPMKPNEVVLGSLLAACRTYGDVSLAE 416

Query: 361 RLMKHLSKLDPGGDSNYVLLSNIYAAIGKWEGANKVRRTMKARGVQKKPGFSSVEIDGKV 420
           +LMKHLSKLDP GDSNYVLLSNIYAAIG+WEGANKVRRTMKARGVQKKPG SSVEIDGKV
Sbjct: 417 KLMKHLSKLDPRGDSNYVLLSNIYAAIGRWEGANKVRRTMKARGVQKKPGCSSVEIDGKV 476

Query: 421 HEFVAGDKYHADADSIYSMLELLVHELKICGSVPETETFLNTKE 465
           HEFVAGDKYHADAD+IYSMLELL HELKI G VP+T   LNTKE
Sbjct: 477 HEFVAGDKYHADADNIYSMLELLFHELKIYGYVPDTNIILNTKE 520

BLAST of MS002863 vs. NCBI nr
Match: KAG7012718.1 (Pentatricopeptide repeat-containing protein, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 844.3 bits (2180), Expect = 4.8e-241
Identity = 404/464 (87.07%), Postives = 435/464 (93.75%), Query Frame = 0

Query: 1   IVLWTSSIARYCRNGQLAEAAAEFTSMRLARVEPNHVTLITLLSGCADFPSESLYFGSSL 60
           IVLWTSSIARYCRN QLAEAAAEFT MRLA VEPNH+T ITLLSGCADFPS SL+FG+SL
Sbjct: 42  IVLWTSSIARYCRNAQLAEAAAEFTRMRLAGVEPNHITFITLLSGCADFPSHSLHFGASL 101

Query: 61  HGYARKLGLDTAHVMVGTSIVDMYAKCAQLGLARRVFDYLRMKNSVSWNTMLDGYTRNGE 120
           HGY RKLGLDT HVMVGT+++ MYAKCAQLGLAR VFDYL MKNSV+WNTMLDGY RNGE
Sbjct: 102 HGYVRKLGLDTGHVMVGTALIAMYAKCAQLGLARNVFDYLDMKNSVTWNTMLDGYMRNGE 161

Query: 121 IELAIDLFDEMPTRDAISWTALINGLLKQGYSEQALECFHQMQCSGIKPDYVSIIAVLAA 180
           IELAI+LFDEMPTRDAISWTALING LKQGYSEQAL+CFH+MQCSGI+PDYVSIIAVLAA
Sbjct: 162 IELAIELFDEMPTRDAISWTALINGFLKQGYSEQALDCFHEMQCSGIEPDYVSIIAVLAA 221

Query: 181 CADLGTLTLGLWVNRFVMQQEFKDNIRISNSLIDMYSRCGCIGFARQVFERMSKRTLVSW 240
           CADLG L+ GLWVNRF+MQQEFKDNIRISNSLIDMYSRCGCI F+RQVF++M KRTLVSW
Sbjct: 222 CADLGALSFGLWVNRFLMQQEFKDNIRISNSLIDMYSRCGCIEFSRQVFDKMPKRTLVSW 281

Query: 241 NSIIVGYAANGFADESLEFFDAMQKEGFKPDAVSYTGALTACSHAGLVNKGLELFDNMKR 300
           NS+IVG+A NGFADESLEFFDAMQKEGFK D VSYTGALTACSHAGLVNKGLELFDNMKR
Sbjct: 282 NSMIVGFAINGFADESLEFFDAMQKEGFKADGVSYTGALTACSHAGLVNKGLELFDNMKR 341

Query: 301 VHRIIPRIEHYGCIVDLYGRAGRLEDALDVIEKMPMKPNEVVLGSLLAACRTHGDVSLAE 360
           VHRI PRIEHYGCIVDLY RAGRL++AL+VIE MPMKPNEVVLGSLLAACRTHGDVSLAE
Sbjct: 342 VHRITPRIEHYGCIVDLYSRAGRLDEALNVIETMPMKPNEVVLGSLLAACRTHGDVSLAE 401

Query: 361 RLMKHLSKLDPGGDSNYVLLSNIYAAIGKWEGANKVRRTMKARGVQKKPGFSSVEIDGKV 420
           RL+K+L +LDPGGDS+YVLLSNIYAA+G+WEGAN VRRTMKARGVQKKPGFSS+EIDGKV
Sbjct: 402 RLIKYLFELDPGGDSSYVLLSNIYAAVGRWEGANMVRRTMKARGVQKKPGFSSIEIDGKV 461

Query: 421 HEFVAGDKYHADADSIYSMLELLVHELKICGSVPETETFLNTKE 465
           HEFVAGDKYHADAD+IYSMLE+L HELKICG VPET TF+N  E
Sbjct: 462 HEFVAGDKYHADADNIYSMLEVLFHELKICGYVPETATFMNGNE 505

BLAST of MS002863 vs. NCBI nr
Match: XP_023521260.1 (pentatricopeptide repeat-containing protein At1g05750, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 844.3 bits (2180), Expect = 4.8e-241
Identity = 405/464 (87.28%), Postives = 434/464 (93.53%), Query Frame = 0

Query: 1   IVLWTSSIARYCRNGQLAEAAAEFTSMRLARVEPNHVTLITLLSGCADFPSESLYFGSSL 60
           IVLWTSSIARYCRN QL EAAAEFT MRLA VEPNH+T ITLLSGCADFPS SL+FG+SL
Sbjct: 42  IVLWTSSIARYCRNDQLPEAAAEFTRMRLAGVEPNHITFITLLSGCADFPSHSLHFGASL 101

Query: 61  HGYARKLGLDTAHVMVGTSIVDMYAKCAQLGLARRVFDYLRMKNSVSWNTMLDGYTRNGE 120
           HGY RKLGLDT HVMVGT+++ MYAKCAQLGLAR VFDYL MKNSV+WNTMLDGY RNGE
Sbjct: 102 HGYVRKLGLDTGHVMVGTALIAMYAKCAQLGLARNVFDYLDMKNSVTWNTMLDGYMRNGE 161

Query: 121 IELAIDLFDEMPTRDAISWTALINGLLKQGYSEQALECFHQMQCSGIKPDYVSIIAVLAA 180
           IELAI+LFDEMPTRDAISWTALING LKQGYSEQALECFH+MQCSGI+PDYVSIIAVLAA
Sbjct: 162 IELAIELFDEMPTRDAISWTALINGFLKQGYSEQALECFHEMQCSGIEPDYVSIIAVLAA 221

Query: 181 CADLGTLTLGLWVNRFVMQQEFKDNIRISNSLIDMYSRCGCIGFARQVFERMSKRTLVSW 240
           CADLG L+ GLWVNRF+MQQEFKDNIRISNSLIDMYSRCGCI FARQVF++M KRTLVSW
Sbjct: 222 CADLGALSFGLWVNRFLMQQEFKDNIRISNSLIDMYSRCGCIEFARQVFDKMPKRTLVSW 281

Query: 241 NSIIVGYAANGFADESLEFFDAMQKEGFKPDAVSYTGALTACSHAGLVNKGLELFDNMKR 300
           NS+IVG+A NGFADESLEFFDAMQKEGFK D VSYTGALTACSHAGLVNKGLELFDNMKR
Sbjct: 282 NSMIVGFAINGFADESLEFFDAMQKEGFKADGVSYTGALTACSHAGLVNKGLELFDNMKR 341

Query: 301 VHRIIPRIEHYGCIVDLYGRAGRLEDALDVIEKMPMKPNEVVLGSLLAACRTHGDVSLAE 360
           VHRI PRIEHYGCIVDLY RAGRL++AL+VIE MPMKPNEVVLGSLLAACRTHGDVSLAE
Sbjct: 342 VHRITPRIEHYGCIVDLYSRAGRLDEALNVIETMPMKPNEVVLGSLLAACRTHGDVSLAE 401

Query: 361 RLMKHLSKLDPGGDSNYVLLSNIYAAIGKWEGANKVRRTMKARGVQKKPGFSSVEIDGKV 420
           RL+K+L +LDPGGDS+YVLLSNIYAA+G+WEGANKVRRTMKARGVQKKPGFSS+EIDGKV
Sbjct: 402 RLIKYLFELDPGGDSSYVLLSNIYAAVGRWEGANKVRRTMKARGVQKKPGFSSIEIDGKV 461

Query: 421 HEFVAGDKYHADADSIYSMLELLVHELKICGSVPETETFLNTKE 465
           HEFVAGDKYHADAD+IYSMLE+L HELKICG VPET T +N  E
Sbjct: 462 HEFVAGDKYHADADNIYSMLEVLFHELKICGYVPETATLMNGNE 505

BLAST of MS002863 vs. NCBI nr
Match: XP_022945035.1 (pentatricopeptide repeat-containing protein At1g05750, chloroplastic [Cucurbita moschata])

HSP 1 Score: 844.0 bits (2179), Expect = 6.3e-241
Identity = 404/464 (87.07%), Postives = 434/464 (93.53%), Query Frame = 0

Query: 1   IVLWTSSIARYCRNGQLAEAAAEFTSMRLARVEPNHVTLITLLSGCADFPSESLYFGSSL 60
           IVLWTSSIARYCRN QL EAAAEFT MRLA VEPNH+T ITLLSGCADFPS SL+FG+SL
Sbjct: 42  IVLWTSSIARYCRNAQLPEAAAEFTRMRLAGVEPNHITFITLLSGCADFPSHSLHFGASL 101

Query: 61  HGYARKLGLDTAHVMVGTSIVDMYAKCAQLGLARRVFDYLRMKNSVSWNTMLDGYTRNGE 120
           HGY RKLGLDT HVMVGT+++ MYAKCAQLGLAR VFDYL MKNSV+WNTMLDGY RNGE
Sbjct: 102 HGYVRKLGLDTGHVMVGTALIAMYAKCAQLGLARNVFDYLDMKNSVTWNTMLDGYMRNGE 161

Query: 121 IELAIDLFDEMPTRDAISWTALINGLLKQGYSEQALECFHQMQCSGIKPDYVSIIAVLAA 180
           IELAI+LFDEMPTRDAISWTALING LKQGYSEQAL+CFH+MQCSGI+PDYVSIIAVLAA
Sbjct: 162 IELAIELFDEMPTRDAISWTALINGFLKQGYSEQALDCFHEMQCSGIEPDYVSIIAVLAA 221

Query: 181 CADLGTLTLGLWVNRFVMQQEFKDNIRISNSLIDMYSRCGCIGFARQVFERMSKRTLVSW 240
           CADLG L+ GLWVNRF+MQQEFKDNIRISNSLIDMYSRCGCI FARQVF++M KRTLVSW
Sbjct: 222 CADLGALSFGLWVNRFLMQQEFKDNIRISNSLIDMYSRCGCIEFARQVFDKMPKRTLVSW 281

Query: 241 NSIIVGYAANGFADESLEFFDAMQKEGFKPDAVSYTGALTACSHAGLVNKGLELFDNMKR 300
           NS+IVG+A NGFADESLEFFDAMQKEGFK D VSYTGALTACSHAGLVNKGLELFDNMKR
Sbjct: 282 NSMIVGFATNGFADESLEFFDAMQKEGFKADGVSYTGALTACSHAGLVNKGLELFDNMKR 341

Query: 301 VHRIIPRIEHYGCIVDLYGRAGRLEDALDVIEKMPMKPNEVVLGSLLAACRTHGDVSLAE 360
           VHRI PRIEHYGCIVDLY RAGRL++AL+VIE MPMKPNEVVLGSLLAACRTHGDVSLAE
Sbjct: 342 VHRITPRIEHYGCIVDLYSRAGRLDEALNVIETMPMKPNEVVLGSLLAACRTHGDVSLAE 401

Query: 361 RLMKHLSKLDPGGDSNYVLLSNIYAAIGKWEGANKVRRTMKARGVQKKPGFSSVEIDGKV 420
           RL+K+L +LDPGGDS+YVLLSNIYAA+G+WEGAN VRRTMKARGVQKKPGFSS+EIDGKV
Sbjct: 402 RLIKYLFELDPGGDSSYVLLSNIYAAVGRWEGANMVRRTMKARGVQKKPGFSSIEIDGKV 461

Query: 421 HEFVAGDKYHADADSIYSMLELLVHELKICGSVPETETFLNTKE 465
           HEFVAGDKYHADAD+IYSMLE+L HELKICG VPET TF+N  E
Sbjct: 462 HEFVAGDKYHADADNIYSMLEVLFHELKICGYVPETATFMNGNE 505

BLAST of MS002863 vs. ExPASy Swiss-Prot
Match: Q9MA50 (Pentatricopeptide repeat-containing protein At1g05750, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PDE247 PE=2 SV=1)

HSP 1 Score: 595.9 bits (1535), Expect = 3.9e-169
Identity = 286/456 (62.72%), Postives = 355/456 (77.85%), Query Frame = 0

Query: 2   VLWTSSIARYCRNGQLAEAAAEFTSMRLARVEPNHVTLITLLSGCADFPSESLYFGSSLH 61
           V WTS I    RNG+LAEAA EF+ M LA VEPNH+T I LLSGC DF S S   G  LH
Sbjct: 37  VSWTSRINLLTRNGRLAEAAKEFSDMTLAGVEPNHITFIALLSGCGDFTSGSEALGDLLH 96

Query: 62  GYARKLGLDTAHVMVGTSIVDMYAKCAQLGLARRVFDYLRMKNSVSWNTMLDGYTRNGEI 121
           GYA KLGLD  HVMVGT+I+ MY+K  +   AR VFDY+  KNSV+WNTM+DGY R+G++
Sbjct: 97  GYACKLGLDRNHVMVGTAIIGMYSKRGRFKKARLVFDYMEDKNSVTWNTMIDGYMRSGQV 156

Query: 122 ELAIDLFDEMPTRDAISWTALINGLLKQGYSEQALECFHQMQCSGIKPDYVSIIAVLAAC 181
           + A  +FD+MP RD ISWTA+ING +K+GY E+AL  F +MQ SG+KPDYV+IIA L AC
Sbjct: 157 DNAAKMFDKMPERDLISWTAMINGFVKKGYQEEALLWFREMQISGVKPDYVAIIAALNAC 216

Query: 182 ADLGTLTLGLWVNRFVMQQEFKDNIRISNSLIDMYSRCGCIGFARQVFERMSKRTLVSWN 241
            +LG L+ GLWV+R+V+ Q+FK+N+R+SNSLID+Y RCGC+ FARQVF  M KRT+VSWN
Sbjct: 217 TNLGALSFGLWVHRYVLSQDFKNNVRVSNSLIDLYCRCGCVEFARQVFYNMEKRTVVSWN 276

Query: 242 SIIVGYAANGFADESLEFFDAMQKEGFKPDAVSYTGALTACSHAGLVNKGLELFDNMKRV 301
           S+IVG+AANG A ESL +F  MQ++GFKPDAV++TGALTACSH GLV +GL  F  MK  
Sbjct: 277 SVIVGFAANGNAHESLVYFRKMQEKGFKPDAVTFTGALTACSHVGLVEEGLRYFQIMKCD 336

Query: 302 HRIIPRIEHYGCIVDLYGRAGRLEDALDVIEKMPMKPNEVVLGSLLAACRTHG-DVSLAE 361
           +RI PRIEHYGC+VDLY RAGRLEDAL +++ MPMKPNEVV+GSLLAAC  HG ++ LAE
Sbjct: 337 YRISPRIEHYGCLVDLYSRAGRLEDALKLVQSMPMKPNEVVIGSLLAACSNHGNNIVLAE 396

Query: 362 RLMKHLSKLDPGGDSNYVLLSNIYAAIGKWEGANKVRRTMKARGVQKKPGFSSVEIDGKV 421
           RLMKHL+ L+    SNYV+LSN+YAA GKWEGA+K+RR MK  G++K+PGFSS+EID  +
Sbjct: 397 RLMKHLTDLNVKSHSNYVILSNMYAADGKWEGASKMRRKMKGLGLKKQPGFSSIEIDDCM 456

Query: 422 HEFVAGDKYHADADSIYSMLELLVHELKICGSVPET 457
           H F+AGD  H +   I  +LEL+  +L++ G V ET
Sbjct: 457 HVFMAGDNAHVETTYIREVLELISSDLRLQGCVVET 492

BLAST of MS002863 vs. ExPASy Swiss-Prot
Match: Q9LUJ2 (Pentatricopeptide repeat-containing protein At3g22690 OS=Arabidopsis thaliana OX=3702 GN=PCMP-H56 PE=3 SV=1)

HSP 1 Score: 365.9 bits (938), Expect = 6.6e-100
Identity = 174/459 (37.91%), Postives = 281/459 (61.22%), Query Frame = 0

Query: 3   LWTSSIARYCRNGQLAEAAAEFTSMRLARVEPNHVTLITLLSGCADFPSESLYFGSSLHG 62
           L  +  + Y R G   EA   F  M  + V P+ +++++ +S C+     ++ +G S HG
Sbjct: 304 LCNAMASNYVRQGLTREALGVFNLMMDSGVRPDRISMLSAISSCSQL--RNILWGKSCHG 363

Query: 63  YARKLGLDTAHVMVGTSIVDMYAKCAQLGLARRVFDYLRMKNSVSWNTMLDGYTRNGEIE 122
           Y  + G ++    +  +++DMY KC +   A R+FD +  K  V+WN+++ GY  NGE++
Sbjct: 364 YVLRNGFESWD-NICNALIDMYMKCHRQDTAFRIFDRMSNKTVVTWNSIVAGYVENGEVD 423

Query: 123 LAIDLFDEMPTRDAISWTALINGLLKQGYSEQALECFHQMQC-SGIKPDYVSIIAVLAAC 182
            A + F+ MP ++ +SW  +I+GL++    E+A+E F  MQ   G+  D V+++++ +AC
Sbjct: 424 AAWETFETMPEKNIVSWNTIISGLVQGSLFEEAIEVFCSMQSQEGVNADGVTMMSIASAC 483

Query: 183 ADLGTLTLGLWVNRFVMQQEFKDNIRISNSLIDMYSRCGCIGFARQVFERMSKRTLVSWN 242
             LG L L  W+  ++ +   + ++R+  +L+DM+SRCG    A  +F  ++ R + +W 
Sbjct: 484 GHLGALDLAKWIYYYIEKNGIQLDVRLGTTLVDMFSRCGDPESAMSIFNSLTNRDVSAWT 543

Query: 243 SIIVGYAANGFADESLEFFDAMQKEGFKPDAVSYTGALTACSHAGLVNKGLELFDNMKRV 302
           + I   A  G A+ ++E FD M ++G KPD V++ GALTACSH GLV +G E+F +M ++
Sbjct: 544 AAIGAMAMAGNAERAIELFDDMIEQGLKPDGVAFVGALTACSHGGLVQQGKEIFYSMLKL 603

Query: 303 HRIIPRIEHYGCIVDLYGRAGRLEDALDVIEKMPMKPNEVVLGSLLAACRTHGDVSLAER 362
           H + P   HYGC+VDL GRAG LE+A+ +IE MPM+PN+V+  SLLAACR  G+V +A  
Sbjct: 604 HGVSPEDVHYGCMVDLLGRAGLLEEAVQLIEDMPMEPNDVIWNSLLAACRVQGNVEMAAY 663

Query: 363 LMKHLSKLDPGGDSNYVLLSNIYAAIGKWEGANKVRRTMKARGVQKKPGFSSVEIDGKVH 422
             + +  L P    +YVLLSN+YA+ G+W    KVR +MK +G++K PG SS++I GK H
Sbjct: 664 AAEKIQVLAPERTGSYVLLSNVYASAGRWNDMAKVRLSMKEKGLRKPPGTSSIQIRGKTH 723

Query: 423 EFVAGDKYHADADSIYSMLELLVHELKICGSVPETETFL 461
           EF +GD+ H +  +I +ML+ +       G VP+    L
Sbjct: 724 EFTSGDESHPEMPNIEAMLDEVSQRASHLGHVPDLSNVL 759

BLAST of MS002863 vs. ExPASy Swiss-Prot
Match: Q9LSB8 (Putative pentatricopeptide repeat-containing protein At3g15930 OS=Arabidopsis thaliana OX=3702 GN=PCMP-E51 PE=3 SV=2)

HSP 1 Score: 365.5 bits (937), Expect = 8.7e-100
Identity = 170/457 (37.20%), Postives = 276/457 (60.39%), Query Frame = 0

Query: 4   WTSSIARYCRNGQLAEAAAEFTSMRLARVEPNHVTLITLLSGCADFPSESLYFGSSLHGY 63
           W   I+ Y R  +  E+      M    V P  VTL+ +LS C+    + L     +H Y
Sbjct: 204 WNLMISGYNRMKEYEESIELLVEMERNLVSPTSVTLLLVLSACSKVKDKDLC--KRVHEY 263

Query: 64  ARKLGLDTAHVMVGTSIVDMYAKCAQLGLARRVFDYLRMKNSVSWNTMLDGYTRNGEIEL 123
             +   + + + +  ++V+ YA C ++ +A R+F  ++ ++ +SW +++ GY   G ++L
Sbjct: 264 VSECKTEPS-LRLENALVNAYAACGEMDIAVRIFRSMKARDVISWTSIVKGYVERGNLKL 323

Query: 124 AIDLFDEMPTRDAISWTALINGLLKQGYSEQALECFHQMQCSGIKPDYVSIIAVLAACAD 183
           A   FD+MP RD ISWT +I+G L+ G   ++LE F +MQ +G+ PD  ++++VL ACA 
Sbjct: 324 ARTYFDQMPVRDRISWTIMIDGYLRAGCFNESLEIFREMQSAGMIPDEFTMVSVLTACAH 383

Query: 184 LGTLTLGLWVNRFVMQQEFKDNIRISNSLIDMYSRCGCIGFARQVFERMSKRTLVSWNSI 243
           LG+L +G W+  ++ + + K+++ + N+LIDMY +CGC   A++VF  M +R   +W ++
Sbjct: 384 LGSLEIGEWIKTYIDKNKIKNDVVVGNALIDMYFKCGCSEKAQKVFHDMDQRDKFTWTAM 443

Query: 244 IVGYAANGFADESLEFFDAMQKEGFKPDAVSYTGALTACSHAGLVNKGLELFDNMKRVHR 303
           +VG A NG   E+++ F  MQ    +PD ++Y G L+AC+H+G+V++  + F  M+  HR
Sbjct: 444 VVGLANNGQGQEAIKVFFQMQDMSIQPDDITYLGVLSACNHSGMVDQARKFFAKMRSDHR 503

Query: 304 IIPRIEHYGCIVDLYGRAGRLEDALDVIEKMPMKPNEVVLGSLLAACRTHGDVSLAERLM 363
           I P + HYGC+VD+ GRAG +++A +++ KMPM PN +V G+LL A R H D  +AE   
Sbjct: 504 IEPSLVHYGCMVDMLGRAGLVKEAYEILRKMPMNPNSIVWGALLGASRLHNDEPMAELAA 563

Query: 364 KHLSKLDPGGDSNYVLLSNIYAAIGKWEGANKVRRTMKARGVQKKPGFSSVEIDGKVHEF 423
           K + +L+P   + Y LL NIYA   +W+   +VRR +    ++K PGFS +E++G  HEF
Sbjct: 564 KKILELEPDNGAVYALLCNIYAGCKRWKDLREVRRKIVDVAIKKTPGFSLIEVNGFAHEF 623

Query: 424 VAGDKYHADADSIYSMLELLVHELKICGSVPETETFL 461
           VAGDK H  ++ IY  LE L  E      +P+T   L
Sbjct: 624 VAGDKSHLQSEEIYMKLEELAQESTFAAYLPDTSELL 657

BLAST of MS002863 vs. ExPASy Swiss-Prot
Match: Q9SIT7 (Pentatricopeptide repeat-containing protein At2g13600 OS=Arabidopsis thaliana OX=3702 GN=PCMP-E76 PE=3 SV=1)

HSP 1 Score: 360.1 bits (923), Expect = 3.6e-98
Identity = 179/454 (39.43%), Postives = 276/454 (60.79%), Query Frame = 0

Query: 1   IVLWTSSIARYCRNGQLAEAAAEFTSMRLARVEPNHVTLITLLSGCADFPSESLYFGSSL 60
           +V W S I  + +NG   EA   F  M  +RVEP+ VTL +++S CA     ++  G  +
Sbjct: 218 VVSWNSLITCFEQNGPAVEALDVFQMMLESRVEPDEVTLASVISACASL--SAIKVGQEV 277

Query: 61  HGYARKLGLDTAHVMVGTSIVDMYAKCAQLGLARRVFDYLRMKNSVSWNTMLDGYTRNGE 120
           HG   K       +++  + VDMYAKC+++  AR +FD + ++N ++  +M+ GY     
Sbjct: 278 HGRVVKNDKLRNDIILSNAFVDMYAKCSRIKEARFIFDSMPIRNVIAETSMISGYAMAAS 337

Query: 121 IELAIDLFDEMPTRDAISWTALINGLLKQGYSEQALECFHQMQCSGIKPDYVSIIAVLAA 180
            + A  +F +M  R+ +SW ALI G  + G +E+AL  F  ++   + P + S   +L A
Sbjct: 338 TKAARLMFTKMAERNVVSWNALIAGYTQNGENEEALSLFCLLKRESVCPTHYSFANILKA 397

Query: 181 CADLGTLTLGLWVNRFVMQQEFK------DNIRISNSLIDMYSRCGCIGFARQVFERMSK 240
           CADL  L LG+  +  V++  FK      D+I + NSLIDMY +CGC+     VF +M +
Sbjct: 398 CADLAELHLGMQAHVHVLKHGFKFQSGEEDDIFVGNSLIDMYVKCGCVEEGYLVFRKMME 457

Query: 241 RTLVSWNSIIVGYAANGFADESLEFFDAMQKEGFKPDAVSYTGALTACSHAGLVNKGLEL 300
           R  VSWN++I+G+A NG+ +E+LE F  M + G KPD ++  G L+AC HAG V +G   
Sbjct: 458 RDCVSWNAMIIGFAQNGYGNEALELFREMLESGEKPDHITMIGVLSACGHAGFVEEGRHY 517

Query: 301 FDNMKRVHRIIPRIEHYGCIVDLYGRAGRLEDALDVIEKMPMKPNEVVLGSLLAACRTHG 360
           F +M R   + P  +HY C+VDL GRAG LE+A  +IE+MPM+P+ V+ GSLLAAC+ H 
Sbjct: 518 FSSMTRDFGVAPLRDHYTCMVDLLGRAGFLEEAKSMIEEMPMQPDSVIWGSLLAACKVHR 577

Query: 361 DVSLAERLMKHLSKLDPGGDSNYVLLSNIYAAIGKWEGANKVRRTMKARGVQKKPGFSSV 420
           +++L + + + L +++P     YVLLSN+YA +GKWE    VR++M+  GV K+PG S +
Sbjct: 578 NITLGKYVAEKLLEVEPSNSGPYVLLSNMYAELGKWEDVMNVRKSMRKEGVTKQPGCSWI 637

Query: 421 EIDGKVHEFVAGDKYHADADSIYSMLELLVHELK 449
           +I G  H F+  DK H     I+S+L++L+ E++
Sbjct: 638 KIQGHDHVFMVKDKSHPRKKQIHSLLDILIAEMR 669

BLAST of MS002863 vs. ExPASy Swiss-Prot
Match: O82380 (Pentatricopeptide repeat-containing protein At2g29760, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PCMP-H33 PE=2 SV=1)

HSP 1 Score: 358.2 bits (918), Expect = 1.4e-97
Identity = 171/465 (36.77%), Postives = 286/465 (61.51%), Query Frame = 0

Query: 1   IVLWTSSIARYCRNGQLAEAAAEFTSMRLARVEPNHVTLITLLSGCADFPSESLYFGSSL 60
           +V W S I  + + G   +A   F  M    V+ +HVT++ +LS CA     +L FG  +
Sbjct: 197 VVSWNSMINGFVQKGSPDKALELFKKMESEDVKASHVTMVGVLSACAKI--RNLEFGRQV 256

Query: 61  HGYARKLGLDTAHVMVGTSIVDMYAKCAQLGLARRVFDYLRMKNSVSWNTMLDGYTRNGE 120
             Y  +  ++  ++ +  +++DMY KC  +  A+R+FD +  K++V+W TMLDGY  + +
Sbjct: 257 CSYIEENRVN-VNLTLANAMLDMYTKCGSIEDAKRLFDAMEEKDNVTWTTMLDGYAISED 316

Query: 121 IELAIDLFDEMPTRDAISWTALINGLLKQGYSEQALECFHQMQC-SGIKPDYVSIIAVLA 180
            E A ++ + MP +D ++W ALI+   + G   +AL  FH++Q    +K + +++++ L+
Sbjct: 317 YEAAREVLNSMPQKDIVAWNALISAYEQNGKPNEALIVFHELQLQKNMKLNQITLVSTLS 376

Query: 181 ACADLGTLTLGLWVNRFVMQQEFKDNIRISNSLIDMYSRCGCIGFARQVFERMSKRTLVS 240
           ACA +G L LG W++ ++ +   + N  ++++LI MYS+CG +  +R+VF  + KR +  
Sbjct: 377 ACAQVGALELGRWIHSYIKKHGIRMNFHVTSALIHMYSKCGDLEKSREVFNSVEKRDVFV 436

Query: 241 WNSIIVGYAANGFADESLEFFDAMQKEGFKPDAVSYTGALTACSHAGLVNKGLELFDNMK 300
           W+++I G A +G  +E+++ F  MQ+   KP+ V++T    ACSH GLV++   LF  M+
Sbjct: 437 WSAMIGGLAMHGCGNEAVDMFYKMQEANVKPNGVTFTNVFCACSHTGLVDEAESLFHQME 496

Query: 301 RVHRIIPRIEHYGCIVDLYGRAGRLEDALDVIEKMPMKPNEVVLGSLLAACRTHGDVSLA 360
             + I+P  +HY CIVD+ GR+G LE A+  IE MP+ P+  V G+LL AC+ H +++LA
Sbjct: 497 SNYGIVPEEKHYACIVDVLGRSGYLEKAVKFIEAMPIPPSTSVWGALLGACKIHANLNLA 556

Query: 361 ERLMKHLSKLDPGGDSNYVLLSNIYAAIGKWEGANKVRRTMKARGVQKKPGFSSVEIDGK 420
           E     L +L+P  D  +VLLSNIYA +GKWE  +++R+ M+  G++K+PG SS+EIDG 
Sbjct: 557 EMACTRLLELEPRNDGAHVLLSNIYAKLGKWENVSELRKHMRVTGLKKEPGCSSIEIDGM 616

Query: 421 VHEFVAGDKYHADADSIYSMLELLVHELKICGSVPETETFLNTKE 465
           +HEF++GD  H  ++ +Y  L  ++ +LK  G  PE    L   E
Sbjct: 617 IHEFLSGDNAHPMSEKVYGKLHEVMEKLKSNGYEPEISQVLQIIE 658

BLAST of MS002863 vs. ExPASy TrEMBL
Match: A0A6J1CN07 (pentatricopeptide repeat-containing protein At1g05750, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111012760 PE=4 SV=1)

HSP 1 Score: 941.0 bits (2431), Expect = 1.8e-270
Identity = 462/464 (99.57%), Postives = 463/464 (99.78%), Query Frame = 0

Query: 1   IVLWTSSIARYCRNGQLAEAAAEFTSMRLARVEPNHVTLITLLSGCADFPSESLYFGSSL 60
           IVLWTSSIARYCRNGQLAEAAAEFTSMRLA VEPNHVTLITLLSGCADFPSESLYFGSSL
Sbjct: 57  IVLWTSSIARYCRNGQLAEAAAEFTSMRLAGVEPNHVTLITLLSGCADFPSESLYFGSSL 116

Query: 61  HGYARKLGLDTAHVMVGTSIVDMYAKCAQLGLARRVFDYLRMKNSVSWNTMLDGYTRNGE 120
           HGYARKLGLDTAHVMVGTSIVDMYAKCAQLGLARRVFDYLRMKNSVSWNTMLDGYTRNGE
Sbjct: 117 HGYARKLGLDTAHVMVGTSIVDMYAKCAQLGLARRVFDYLRMKNSVSWNTMLDGYTRNGE 176

Query: 121 IELAIDLFDEMPTRDAISWTALINGLLKQGYSEQALECFHQMQCSGIKPDYVSIIAVLAA 180
           IELAIDLFDEMPTRDAISWTALINGLLKQGYSEQALECFHQMQCSGIKPDYVSIIAVLAA
Sbjct: 177 IELAIDLFDEMPTRDAISWTALINGLLKQGYSEQALECFHQMQCSGIKPDYVSIIAVLAA 236

Query: 181 CADLGTLTLGLWVNRFVMQQEFKDNIRISNSLIDMYSRCGCIGFARQVFERMSKRTLVSW 240
           CADLGTLTLGLWVNRFVMQQEFKDNIRISNSLIDMYSRCGCIGFARQVFERMSKRTLVSW
Sbjct: 237 CADLGTLTLGLWVNRFVMQQEFKDNIRISNSLIDMYSRCGCIGFARQVFERMSKRTLVSW 296

Query: 241 NSIIVGYAANGFADESLEFFDAMQKEGFKPDAVSYTGALTACSHAGLVNKGLELFDNMKR 300
           NSIIVGYAANGFADESLEFFDAMQKEGFKPDAVSYTGALTACSHAGLVNKGLELFDNMKR
Sbjct: 297 NSIIVGYAANGFADESLEFFDAMQKEGFKPDAVSYTGALTACSHAGLVNKGLELFDNMKR 356

Query: 301 VHRIIPRIEHYGCIVDLYGRAGRLEDALDVIEKMPMKPNEVVLGSLLAACRTHGDVSLAE 360
           VHRIIPRIEHYGCIVDLYGRAGRLEDALDVIEKMPMKPNEVVLGSLLAACRTHGDVSLAE
Sbjct: 357 VHRIIPRIEHYGCIVDLYGRAGRLEDALDVIEKMPMKPNEVVLGSLLAACRTHGDVSLAE 416

Query: 361 RLMKHLSKLDPGGDSNYVLLSNIYAAIGKWEGANKVRRTMKARGVQKKPGFSSVEIDGKV 420
           RLMKHLSKLDPGGDSNYVLLSNIYAAIGKWEGANKVRRTMKARGVQKKPGFSSVEIDGKV
Sbjct: 417 RLMKHLSKLDPGGDSNYVLLSNIYAAIGKWEGANKVRRTMKARGVQKKPGFSSVEIDGKV 476

Query: 421 HEFVAGDKYHADADSIYSMLELLVHELKICGSVPETETFLNTKE 465
           HEFVAGDKYHADADSIYSML+LLVHELKICGSVPETETFLNTKE
Sbjct: 477 HEFVAGDKYHADADSIYSMLQLLVHELKICGSVPETETFLNTKE 520

BLAST of MS002863 vs. ExPASy TrEMBL
Match: A0A6J1FZR8 (pentatricopeptide repeat-containing protein At1g05750, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111449396 PE=4 SV=1)

HSP 1 Score: 844.0 bits (2179), Expect = 3.1e-241
Identity = 404/464 (87.07%), Postives = 434/464 (93.53%), Query Frame = 0

Query: 1   IVLWTSSIARYCRNGQLAEAAAEFTSMRLARVEPNHVTLITLLSGCADFPSESLYFGSSL 60
           IVLWTSSIARYCRN QL EAAAEFT MRLA VEPNH+T ITLLSGCADFPS SL+FG+SL
Sbjct: 42  IVLWTSSIARYCRNAQLPEAAAEFTRMRLAGVEPNHITFITLLSGCADFPSHSLHFGASL 101

Query: 61  HGYARKLGLDTAHVMVGTSIVDMYAKCAQLGLARRVFDYLRMKNSVSWNTMLDGYTRNGE 120
           HGY RKLGLDT HVMVGT+++ MYAKCAQLGLAR VFDYL MKNSV+WNTMLDGY RNGE
Sbjct: 102 HGYVRKLGLDTGHVMVGTALIAMYAKCAQLGLARNVFDYLDMKNSVTWNTMLDGYMRNGE 161

Query: 121 IELAIDLFDEMPTRDAISWTALINGLLKQGYSEQALECFHQMQCSGIKPDYVSIIAVLAA 180
           IELAI+LFDEMPTRDAISWTALING LKQGYSEQAL+CFH+MQCSGI+PDYVSIIAVLAA
Sbjct: 162 IELAIELFDEMPTRDAISWTALINGFLKQGYSEQALDCFHEMQCSGIEPDYVSIIAVLAA 221

Query: 181 CADLGTLTLGLWVNRFVMQQEFKDNIRISNSLIDMYSRCGCIGFARQVFERMSKRTLVSW 240
           CADLG L+ GLWVNRF+MQQEFKDNIRISNSLIDMYSRCGCI FARQVF++M KRTLVSW
Sbjct: 222 CADLGALSFGLWVNRFLMQQEFKDNIRISNSLIDMYSRCGCIEFARQVFDKMPKRTLVSW 281

Query: 241 NSIIVGYAANGFADESLEFFDAMQKEGFKPDAVSYTGALTACSHAGLVNKGLELFDNMKR 300
           NS+IVG+A NGFADESLEFFDAMQKEGFK D VSYTGALTACSHAGLVNKGLELFDNMKR
Sbjct: 282 NSMIVGFATNGFADESLEFFDAMQKEGFKADGVSYTGALTACSHAGLVNKGLELFDNMKR 341

Query: 301 VHRIIPRIEHYGCIVDLYGRAGRLEDALDVIEKMPMKPNEVVLGSLLAACRTHGDVSLAE 360
           VHRI PRIEHYGCIVDLY RAGRL++AL+VIE MPMKPNEVVLGSLLAACRTHGDVSLAE
Sbjct: 342 VHRITPRIEHYGCIVDLYSRAGRLDEALNVIETMPMKPNEVVLGSLLAACRTHGDVSLAE 401

Query: 361 RLMKHLSKLDPGGDSNYVLLSNIYAAIGKWEGANKVRRTMKARGVQKKPGFSSVEIDGKV 420
           RL+K+L +LDPGGDS+YVLLSNIYAA+G+WEGAN VRRTMKARGVQKKPGFSS+EIDGKV
Sbjct: 402 RLIKYLFELDPGGDSSYVLLSNIYAAVGRWEGANMVRRTMKARGVQKKPGFSSIEIDGKV 461

Query: 421 HEFVAGDKYHADADSIYSMLELLVHELKICGSVPETETFLNTKE 465
           HEFVAGDKYHADAD+IYSMLE+L HELKICG VPET TF+N  E
Sbjct: 462 HEFVAGDKYHADADNIYSMLEVLFHELKICGYVPETATFMNGNE 505

BLAST of MS002863 vs. ExPASy TrEMBL
Match: A0A6J1HU31 (pentatricopeptide repeat-containing protein At1g05750, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111466586 PE=4 SV=1)

HSP 1 Score: 841.3 bits (2172), Expect = 2.0e-240
Identity = 404/464 (87.07%), Postives = 433/464 (93.32%), Query Frame = 0

Query: 1   IVLWTSSIARYCRNGQLAEAAAEFTSMRLARVEPNHVTLITLLSGCADFPSESLYFGSSL 60
           IVLWTSSIARYCRN QLAEAAAEFT MRLA VEPNH+T ITLLSGCADFPS SL+FG+SL
Sbjct: 49  IVLWTSSIARYCRNAQLAEAAAEFTRMRLAGVEPNHITFITLLSGCADFPSHSLHFGASL 108

Query: 61  HGYARKLGLDTAHVMVGTSIVDMYAKCAQLGLARRVFDYLRMKNSVSWNTMLDGYTRNGE 120
           HGY RKLGLDT HVMVGT+++ MYAKCAQLGLAR VFDYL MKNSV+WNTMLDGY RNGE
Sbjct: 109 HGYVRKLGLDTGHVMVGTALIAMYAKCAQLGLARNVFDYLAMKNSVTWNTMLDGYMRNGE 168

Query: 121 IELAIDLFDEMPTRDAISWTALINGLLKQGYSEQALECFHQMQCSGIKPDYVSIIAVLAA 180
           IELAI+LFDEMPTRDAISWTALING LKQGYSEQALECFH+MQCSGI+PDYVSIIAVLAA
Sbjct: 169 IELAIELFDEMPTRDAISWTALINGFLKQGYSEQALECFHEMQCSGIEPDYVSIIAVLAA 228

Query: 181 CADLGTLTLGLWVNRFVMQQEFKDNIRISNSLIDMYSRCGCIGFARQVFERMSKRTLVSW 240
           CADLG L+ GLWVNRF+MQQEFKDNIRISNSLIDMYSRCGCI FARQVF++MSK TLVSW
Sbjct: 229 CADLGALSFGLWVNRFLMQQEFKDNIRISNSLIDMYSRCGCIEFARQVFDKMSKHTLVSW 288

Query: 241 NSIIVGYAANGFADESLEFFDAMQKEGFKPDAVSYTGALTACSHAGLVNKGLELFDNMKR 300
           NS+IVG+A NGFADESLEFFDAMQKEGF  D VSYTGALTACSHAGLVNKGLELFDNMKR
Sbjct: 289 NSMIVGFAINGFADESLEFFDAMQKEGFMADGVSYTGALTACSHAGLVNKGLELFDNMKR 348

Query: 301 VHRIIPRIEHYGCIVDLYGRAGRLEDALDVIEKMPMKPNEVVLGSLLAACRTHGDVSLAE 360
           VHRI PRIEHYGCIVDLY RAGRL++AL+VIE MPMKPNEVVLGSLLAACRTHGDVSLAE
Sbjct: 349 VHRITPRIEHYGCIVDLYSRAGRLDEALNVIETMPMKPNEVVLGSLLAACRTHGDVSLAE 408

Query: 361 RLMKHLSKLDPGGDSNYVLLSNIYAAIGKWEGANKVRRTMKARGVQKKPGFSSVEIDGKV 420
           RL+K+L +LDPGGDS+YVLLSNIYAA+G+WEGANKVRRTMKARGVQKKPGFSS+EIDGKV
Sbjct: 409 RLIKYLFELDPGGDSSYVLLSNIYAAVGRWEGANKVRRTMKARGVQKKPGFSSIEIDGKV 468

Query: 421 HEFVAGDKYHADADSIYSMLELLVHELKICGSVPETETFLNTKE 465
           HEFVAGDKYH DAD+IYSMLE+L HELKI G VPET TF+N  E
Sbjct: 469 HEFVAGDKYHVDADNIYSMLEVLFHELKIYGYVPETATFMNGNE 512

BLAST of MS002863 vs. ExPASy TrEMBL
Match: A0A5A7UJB6 (Pentatricopeptide repeat-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold302G002000 PE=4 SV=1)

HSP 1 Score: 818.9 bits (2114), Expect = 1.1e-233
Identity = 399/464 (85.99%), Postives = 427/464 (92.03%), Query Frame = 0

Query: 1   IVLWTSSIARYCRNGQLAEAAAEFTSMRLARVEPNHVTLITLLSGCADFPSESLYFGSSL 60
           IV WTSSIARYC NGQL EAAAEFT MRLA VEPNH+T ITLLSGCADFPSES +F SSL
Sbjct: 55  IVQWTSSIARYCGNGQLPEAAAEFTRMRLAGVEPNHITFITLLSGCADFPSES-FFASSL 114

Query: 61  HGYARKLGLDTAHVMVGTSIVDMYAKCAQLGLARRVFDYLRMKNSVSWNTMLDGYTRNGE 120
           HGYA K GLDT HVMVGT+++DMY+KC+QLGLA++VFDYL +KNSVSWNTML+G+ RNGE
Sbjct: 115 HGYACKFGLDTGHVMVGTALIDMYSKCSQLGLAKKVFDYLGVKNSVSWNTMLNGFMRNGE 174

Query: 121 IELAIDLFDEMPTRDAISWTALINGLLKQGYSEQALECFHQMQCSGIKPDYVSIIAVLAA 180
           IELAI LFDEMPTRDAISWTALINGLLK GYSEQALECFHQMQ SG+  DYVSIIAVLAA
Sbjct: 175 IELAIQLFDEMPTRDAISWTALINGLLKHGYSEQALECFHQMQRSGVVADYVSIIAVLAA 234

Query: 181 CADLGTLTLGLWVNRFVMQQEFKDNIRISNSLIDMYSRCGCIGFARQVFERMSKRTLVSW 240
           CADLG LT GLWVNRFVMQQEFKDN+RISNSLIDMYSRCGCI FARQVF +M+KRTLVSW
Sbjct: 235 CADLGALTSGLWVNRFVMQQEFKDNVRISNSLIDMYSRCGCIEFARQVFVKMAKRTLVSW 294

Query: 241 NSIIVGYAANGFADESLEFFDAMQKEGFKPDAVSYTGALTACSHAGLVNKGLELFDNMKR 300
           NSIIVG+A NGFADESLEFF AMQKEGFKPD VSYTGALTACSHAGLVNKGLELFDNMKR
Sbjct: 295 NSIIVGFAFNGFADESLEFFYAMQKEGFKPDGVSYTGALTACSHAGLVNKGLELFDNMKR 354

Query: 301 VHRIIPRIEHYGCIVDLYGRAGRLEDALDVIEKMPMKPNEVVLGSLLAACRTHGDVSLAE 360
           VH+I P IEHYGCIVDLYGRAGRLEDA +VIE+MPMKPNEVVLGSLLAACRTHGDV LAE
Sbjct: 355 VHKITPGIEHYGCIVDLYGRAGRLEDASNVIEEMPMKPNEVVLGSLLAACRTHGDVRLAE 414

Query: 361 RLMKHLSKLDPGGDSNYVLLSNIYAAIGKWEGANKVRRTMKARGVQKKPGFSSVEIDGKV 420
           RLMKH+ KLD  GDSNYVLLSNIYAAIGKWEGANKVRRTMKARGVQKK G+SSVEIDGKV
Sbjct: 415 RLMKHIFKLDSVGDSNYVLLSNIYAAIGKWEGANKVRRTMKARGVQKKRGYSSVEIDGKV 474

Query: 421 HEFVAGDKYHADADSIYSMLELLVHELKICGSVPETETFLNTKE 465
           HEFVAGDKYHADAD+IYSML+LL HELK+CG VP+T+  LNTK+
Sbjct: 475 HEFVAGDKYHADADNIYSMLDLLFHELKVCGYVPDTDIILNTKD 517

BLAST of MS002863 vs. ExPASy TrEMBL
Match: A0A1S3C956 (pentatricopeptide repeat-containing protein At1g05750, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103498199 PE=4 SV=1)

HSP 1 Score: 818.9 bits (2114), Expect = 1.1e-233
Identity = 399/464 (85.99%), Postives = 427/464 (92.03%), Query Frame = 0

Query: 1   IVLWTSSIARYCRNGQLAEAAAEFTSMRLARVEPNHVTLITLLSGCADFPSESLYFGSSL 60
           IV WTSSIARYC NGQL EAAAEFT MRLA VEPNH+T ITLLSGCADFPSES +F SSL
Sbjct: 55  IVQWTSSIARYCGNGQLPEAAAEFTRMRLAGVEPNHITFITLLSGCADFPSES-FFASSL 114

Query: 61  HGYARKLGLDTAHVMVGTSIVDMYAKCAQLGLARRVFDYLRMKNSVSWNTMLDGYTRNGE 120
           HGYA K GLDT HVMVGT+++DMY+KC+QLGLA++VFDYL +KNSVSWNTML+G+ RNGE
Sbjct: 115 HGYACKFGLDTGHVMVGTALIDMYSKCSQLGLAKKVFDYLGVKNSVSWNTMLNGFMRNGE 174

Query: 121 IELAIDLFDEMPTRDAISWTALINGLLKQGYSEQALECFHQMQCSGIKPDYVSIIAVLAA 180
           IELAI LFDEMPTRDAISWTALINGLLK GYSEQALECFHQMQ SG+  DYVSIIAVLAA
Sbjct: 175 IELAIQLFDEMPTRDAISWTALINGLLKHGYSEQALECFHQMQRSGVVADYVSIIAVLAA 234

Query: 181 CADLGTLTLGLWVNRFVMQQEFKDNIRISNSLIDMYSRCGCIGFARQVFERMSKRTLVSW 240
           CADLG LT GLWVNRFVMQQEFKDN+RISNSLIDMYSRCGCI FARQVF +M+KRTLVSW
Sbjct: 235 CADLGALTSGLWVNRFVMQQEFKDNVRISNSLIDMYSRCGCIEFARQVFVKMAKRTLVSW 294

Query: 241 NSIIVGYAANGFADESLEFFDAMQKEGFKPDAVSYTGALTACSHAGLVNKGLELFDNMKR 300
           NSIIVG+A NGFADESLEFF AMQKEGFKPD VSYTGALTACSHAGLVNKGLELFDNMKR
Sbjct: 295 NSIIVGFAFNGFADESLEFFYAMQKEGFKPDGVSYTGALTACSHAGLVNKGLELFDNMKR 354

Query: 301 VHRIIPRIEHYGCIVDLYGRAGRLEDALDVIEKMPMKPNEVVLGSLLAACRTHGDVSLAE 360
           VH+I P IEHYGCIVDLYGRAGRLEDA +VIE+MPMKPNEVVLGSLLAACRTHGDV LAE
Sbjct: 355 VHKITPGIEHYGCIVDLYGRAGRLEDASNVIEEMPMKPNEVVLGSLLAACRTHGDVRLAE 414

Query: 361 RLMKHLSKLDPGGDSNYVLLSNIYAAIGKWEGANKVRRTMKARGVQKKPGFSSVEIDGKV 420
           RLMKH+ KLD  GDSNYVLLSNIYAAIGKWEGANKVRRTMKARGVQKK G+SSVEIDGKV
Sbjct: 415 RLMKHIFKLDSVGDSNYVLLSNIYAAIGKWEGANKVRRTMKARGVQKKRGYSSVEIDGKV 474

Query: 421 HEFVAGDKYHADADSIYSMLELLVHELKICGSVPETETFLNTKE 465
           HEFVAGDKYHADAD+IYSML+LL HELK+CG VP+T+  LNTK+
Sbjct: 475 HEFVAGDKYHADADNIYSMLDLLFHELKVCGYVPDTDIILNTKD 517

BLAST of MS002863 vs. TAIR 10
Match: AT1G05750.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 595.9 bits (1535), Expect = 2.8e-170
Identity = 286/456 (62.72%), Postives = 355/456 (77.85%), Query Frame = 0

Query: 2   VLWTSSIARYCRNGQLAEAAAEFTSMRLARVEPNHVTLITLLSGCADFPSESLYFGSSLH 61
           V WTS I    RNG+LAEAA EF+ M LA VEPNH+T I LLSGC DF S S   G  LH
Sbjct: 37  VSWTSRINLLTRNGRLAEAAKEFSDMTLAGVEPNHITFIALLSGCGDFTSGSEALGDLLH 96

Query: 62  GYARKLGLDTAHVMVGTSIVDMYAKCAQLGLARRVFDYLRMKNSVSWNTMLDGYTRNGEI 121
           GYA KLGLD  HVMVGT+I+ MY+K  +   AR VFDY+  KNSV+WNTM+DGY R+G++
Sbjct: 97  GYACKLGLDRNHVMVGTAIIGMYSKRGRFKKARLVFDYMEDKNSVTWNTMIDGYMRSGQV 156

Query: 122 ELAIDLFDEMPTRDAISWTALINGLLKQGYSEQALECFHQMQCSGIKPDYVSIIAVLAAC 181
           + A  +FD+MP RD ISWTA+ING +K+GY E+AL  F +MQ SG+KPDYV+IIA L AC
Sbjct: 157 DNAAKMFDKMPERDLISWTAMINGFVKKGYQEEALLWFREMQISGVKPDYVAIIAALNAC 216

Query: 182 ADLGTLTLGLWVNRFVMQQEFKDNIRISNSLIDMYSRCGCIGFARQVFERMSKRTLVSWN 241
            +LG L+ GLWV+R+V+ Q+FK+N+R+SNSLID+Y RCGC+ FARQVF  M KRT+VSWN
Sbjct: 217 TNLGALSFGLWVHRYVLSQDFKNNVRVSNSLIDLYCRCGCVEFARQVFYNMEKRTVVSWN 276

Query: 242 SIIVGYAANGFADESLEFFDAMQKEGFKPDAVSYTGALTACSHAGLVNKGLELFDNMKRV 301
           S+IVG+AANG A ESL +F  MQ++GFKPDAV++TGALTACSH GLV +GL  F  MK  
Sbjct: 277 SVIVGFAANGNAHESLVYFRKMQEKGFKPDAVTFTGALTACSHVGLVEEGLRYFQIMKCD 336

Query: 302 HRIIPRIEHYGCIVDLYGRAGRLEDALDVIEKMPMKPNEVVLGSLLAACRTHG-DVSLAE 361
           +RI PRIEHYGC+VDLY RAGRLEDAL +++ MPMKPNEVV+GSLLAAC  HG ++ LAE
Sbjct: 337 YRISPRIEHYGCLVDLYSRAGRLEDALKLVQSMPMKPNEVVIGSLLAACSNHGNNIVLAE 396

Query: 362 RLMKHLSKLDPGGDSNYVLLSNIYAAIGKWEGANKVRRTMKARGVQKKPGFSSVEIDGKV 421
           RLMKHL+ L+    SNYV+LSN+YAA GKWEGA+K+RR MK  G++K+PGFSS+EID  +
Sbjct: 397 RLMKHLTDLNVKSHSNYVILSNMYAADGKWEGASKMRRKMKGLGLKKQPGFSSIEIDDCM 456

Query: 422 HEFVAGDKYHADADSIYSMLELLVHELKICGSVPET 457
           H F+AGD  H +   I  +LEL+  +L++ G V ET
Sbjct: 457 HVFMAGDNAHVETTYIREVLELISSDLRLQGCVVET 492

BLAST of MS002863 vs. TAIR 10
Match: AT3G22690.1 (CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF1685 (InterPro:IPR012881), Pentatricopeptide repeat (InterPro:IPR002885); BEST Arabidopsis thaliana protein match is: Tetratricopeptide repeat (TPR)-like superfamily protein (TAIR:AT2G29760.1); Has 49784 Blast hits to 14716 proteins in 280 species: Archae - 2; Bacteria - 10; Metazoa - 107; Fungi - 167; Plants - 48594; Viruses - 0; Other Eukaryotes - 904 (source: NCBI BLink). )

HSP 1 Score: 365.9 bits (938), Expect = 4.7e-101
Identity = 174/459 (37.91%), Postives = 281/459 (61.22%), Query Frame = 0

Query: 3   LWTSSIARYCRNGQLAEAAAEFTSMRLARVEPNHVTLITLLSGCADFPSESLYFGSSLHG 62
           L  +  + Y R G   EA   F  M  + V P+ +++++ +S C+     ++ +G S HG
Sbjct: 304 LCNAMASNYVRQGLTREALGVFNLMMDSGVRPDRISMLSAISSCSQL--RNILWGKSCHG 363

Query: 63  YARKLGLDTAHVMVGTSIVDMYAKCAQLGLARRVFDYLRMKNSVSWNTMLDGYTRNGEIE 122
           Y  + G ++    +  +++DMY KC +   A R+FD +  K  V+WN+++ GY  NGE++
Sbjct: 364 YVLRNGFESWD-NICNALIDMYMKCHRQDTAFRIFDRMSNKTVVTWNSIVAGYVENGEVD 423

Query: 123 LAIDLFDEMPTRDAISWTALINGLLKQGYSEQALECFHQMQC-SGIKPDYVSIIAVLAAC 182
            A + F+ MP ++ +SW  +I+GL++    E+A+E F  MQ   G+  D V+++++ +AC
Sbjct: 424 AAWETFETMPEKNIVSWNTIISGLVQGSLFEEAIEVFCSMQSQEGVNADGVTMMSIASAC 483

Query: 183 ADLGTLTLGLWVNRFVMQQEFKDNIRISNSLIDMYSRCGCIGFARQVFERMSKRTLVSWN 242
             LG L L  W+  ++ +   + ++R+  +L+DM+SRCG    A  +F  ++ R + +W 
Sbjct: 484 GHLGALDLAKWIYYYIEKNGIQLDVRLGTTLVDMFSRCGDPESAMSIFNSLTNRDVSAWT 543

Query: 243 SIIVGYAANGFADESLEFFDAMQKEGFKPDAVSYTGALTACSHAGLVNKGLELFDNMKRV 302
           + I   A  G A+ ++E FD M ++G KPD V++ GALTACSH GLV +G E+F +M ++
Sbjct: 544 AAIGAMAMAGNAERAIELFDDMIEQGLKPDGVAFVGALTACSHGGLVQQGKEIFYSMLKL 603

Query: 303 HRIIPRIEHYGCIVDLYGRAGRLEDALDVIEKMPMKPNEVVLGSLLAACRTHGDVSLAER 362
           H + P   HYGC+VDL GRAG LE+A+ +IE MPM+PN+V+  SLLAACR  G+V +A  
Sbjct: 604 HGVSPEDVHYGCMVDLLGRAGLLEEAVQLIEDMPMEPNDVIWNSLLAACRVQGNVEMAAY 663

Query: 363 LMKHLSKLDPGGDSNYVLLSNIYAAIGKWEGANKVRRTMKARGVQKKPGFSSVEIDGKVH 422
             + +  L P    +YVLLSN+YA+ G+W    KVR +MK +G++K PG SS++I GK H
Sbjct: 664 AAEKIQVLAPERTGSYVLLSNVYASAGRWNDMAKVRLSMKEKGLRKPPGTSSIQIRGKTH 723

Query: 423 EFVAGDKYHADADSIYSMLELLVHELKICGSVPETETFL 461
           EF +GD+ H +  +I +ML+ +       G VP+    L
Sbjct: 724 EFTSGDESHPEMPNIEAMLDEVSQRASHLGHVPDLSNVL 759

BLAST of MS002863 vs. TAIR 10
Match: AT3G22690.2 (INVOLVED IN: photosystem II assembly, regulation of chlorophyll biosynthetic process, photosystem I assembly, thylakoid membrane organization, RNA modification; LOCATED IN: chloroplast; EXPRESSED IN: 13 plant structures; EXPRESSED DURING: LP.04 four leaves visible, 4 anthesis, petal differentiation and expansion stage, E expanded cotyledon stage, D bilateral stage; CONTAINS InterPro DOMAIN/s: Pentatricopeptide repeat (InterPro:IPR002885); BEST Arabidopsis thaliana protein match is: Tetratricopeptide repeat (TPR)-like superfamily protein (TAIR:AT2G29760.1). )

HSP 1 Score: 365.9 bits (938), Expect = 4.7e-101
Identity = 174/459 (37.91%), Postives = 281/459 (61.22%), Query Frame = 0

Query: 3   LWTSSIARYCRNGQLAEAAAEFTSMRLARVEPNHVTLITLLSGCADFPSESLYFGSSLHG 62
           L  +  + Y R G   EA   F  M  + V P+ +++++ +S C+     ++ +G S HG
Sbjct: 304 LCNAMASNYVRQGLTREALGVFNLMMDSGVRPDRISMLSAISSCSQL--RNILWGKSCHG 363

Query: 63  YARKLGLDTAHVMVGTSIVDMYAKCAQLGLARRVFDYLRMKNSVSWNTMLDGYTRNGEIE 122
           Y  + G ++    +  +++DMY KC +   A R+FD +  K  V+WN+++ GY  NGE++
Sbjct: 364 YVLRNGFESWD-NICNALIDMYMKCHRQDTAFRIFDRMSNKTVVTWNSIVAGYVENGEVD 423

Query: 123 LAIDLFDEMPTRDAISWTALINGLLKQGYSEQALECFHQMQC-SGIKPDYVSIIAVLAAC 182
            A + F+ MP ++ +SW  +I+GL++    E+A+E F  MQ   G+  D V+++++ +AC
Sbjct: 424 AAWETFETMPEKNIVSWNTIISGLVQGSLFEEAIEVFCSMQSQEGVNADGVTMMSIASAC 483

Query: 183 ADLGTLTLGLWVNRFVMQQEFKDNIRISNSLIDMYSRCGCIGFARQVFERMSKRTLVSWN 242
             LG L L  W+  ++ +   + ++R+  +L+DM+SRCG    A  +F  ++ R + +W 
Sbjct: 484 GHLGALDLAKWIYYYIEKNGIQLDVRLGTTLVDMFSRCGDPESAMSIFNSLTNRDVSAWT 543

Query: 243 SIIVGYAANGFADESLEFFDAMQKEGFKPDAVSYTGALTACSHAGLVNKGLELFDNMKRV 302
           + I   A  G A+ ++E FD M ++G KPD V++ GALTACSH GLV +G E+F +M ++
Sbjct: 544 AAIGAMAMAGNAERAIELFDDMIEQGLKPDGVAFVGALTACSHGGLVQQGKEIFYSMLKL 603

Query: 303 HRIIPRIEHYGCIVDLYGRAGRLEDALDVIEKMPMKPNEVVLGSLLAACRTHGDVSLAER 362
           H + P   HYGC+VDL GRAG LE+A+ +IE MPM+PN+V+  SLLAACR  G+V +A  
Sbjct: 604 HGVSPEDVHYGCMVDLLGRAGLLEEAVQLIEDMPMEPNDVIWNSLLAACRVQGNVEMAAY 663

Query: 363 LMKHLSKLDPGGDSNYVLLSNIYAAIGKWEGANKVRRTMKARGVQKKPGFSSVEIDGKVH 422
             + +  L P    +YVLLSN+YA+ G+W    KVR +MK +G++K PG SS++I GK H
Sbjct: 664 AAEKIQVLAPERTGSYVLLSNVYASAGRWNDMAKVRLSMKEKGLRKPPGTSSIQIRGKTH 723

Query: 423 EFVAGDKYHADADSIYSMLELLVHELKICGSVPETETFL 461
           EF +GD+ H +  +I +ML+ +       G VP+    L
Sbjct: 724 EFTSGDESHPEMPNIEAMLDEVSQRASHLGHVPDLSNVL 759

BLAST of MS002863 vs. TAIR 10
Match: AT3G15930.1 (Pentatricopeptide repeat (PPR) superfamily protein )

HSP 1 Score: 365.5 bits (937), Expect = 6.1e-101
Identity = 170/457 (37.20%), Postives = 276/457 (60.39%), Query Frame = 0

Query: 4   WTSSIARYCRNGQLAEAAAEFTSMRLARVEPNHVTLITLLSGCADFPSESLYFGSSLHGY 63
           W   I+ Y R  +  E+      M    V P  VTL+ +LS C+    + L     +H Y
Sbjct: 204 WNLMISGYNRMKEYEESIELLVEMERNLVSPTSVTLLLVLSACSKVKDKDLC--KRVHEY 263

Query: 64  ARKLGLDTAHVMVGTSIVDMYAKCAQLGLARRVFDYLRMKNSVSWNTMLDGYTRNGEIEL 123
             +   + + + +  ++V+ YA C ++ +A R+F  ++ ++ +SW +++ GY   G ++L
Sbjct: 264 VSECKTEPS-LRLENALVNAYAACGEMDIAVRIFRSMKARDVISWTSIVKGYVERGNLKL 323

Query: 124 AIDLFDEMPTRDAISWTALINGLLKQGYSEQALECFHQMQCSGIKPDYVSIIAVLAACAD 183
           A   FD+MP RD ISWT +I+G L+ G   ++LE F +MQ +G+ PD  ++++VL ACA 
Sbjct: 324 ARTYFDQMPVRDRISWTIMIDGYLRAGCFNESLEIFREMQSAGMIPDEFTMVSVLTACAH 383

Query: 184 LGTLTLGLWVNRFVMQQEFKDNIRISNSLIDMYSRCGCIGFARQVFERMSKRTLVSWNSI 243
           LG+L +G W+  ++ + + K+++ + N+LIDMY +CGC   A++VF  M +R   +W ++
Sbjct: 384 LGSLEIGEWIKTYIDKNKIKNDVVVGNALIDMYFKCGCSEKAQKVFHDMDQRDKFTWTAM 443

Query: 244 IVGYAANGFADESLEFFDAMQKEGFKPDAVSYTGALTACSHAGLVNKGLELFDNMKRVHR 303
           +VG A NG   E+++ F  MQ    +PD ++Y G L+AC+H+G+V++  + F  M+  HR
Sbjct: 444 VVGLANNGQGQEAIKVFFQMQDMSIQPDDITYLGVLSACNHSGMVDQARKFFAKMRSDHR 503

Query: 304 IIPRIEHYGCIVDLYGRAGRLEDALDVIEKMPMKPNEVVLGSLLAACRTHGDVSLAERLM 363
           I P + HYGC+VD+ GRAG +++A +++ KMPM PN +V G+LL A R H D  +AE   
Sbjct: 504 IEPSLVHYGCMVDMLGRAGLVKEAYEILRKMPMNPNSIVWGALLGASRLHNDEPMAELAA 563

Query: 364 KHLSKLDPGGDSNYVLLSNIYAAIGKWEGANKVRRTMKARGVQKKPGFSSVEIDGKVHEF 423
           K + +L+P   + Y LL NIYA   +W+   +VRR +    ++K PGFS +E++G  HEF
Sbjct: 564 KKILELEPDNGAVYALLCNIYAGCKRWKDLREVRRKIVDVAIKKTPGFSLIEVNGFAHEF 623

Query: 424 VAGDKYHADADSIYSMLELLVHELKICGSVPETETFL 461
           VAGDK H  ++ IY  LE L  E      +P+T   L
Sbjct: 624 VAGDKSHLQSEEIYMKLEELAQESTFAAYLPDTSELL 657

BLAST of MS002863 vs. TAIR 10
Match: AT2G13600.1 (Pentatricopeptide repeat (PPR) superfamily protein )

HSP 1 Score: 360.1 bits (923), Expect = 2.6e-99
Identity = 179/454 (39.43%), Postives = 276/454 (60.79%), Query Frame = 0

Query: 1   IVLWTSSIARYCRNGQLAEAAAEFTSMRLARVEPNHVTLITLLSGCADFPSESLYFGSSL 60
           +V W S I  + +NG   EA   F  M  +RVEP+ VTL +++S CA     ++  G  +
Sbjct: 218 VVSWNSLITCFEQNGPAVEALDVFQMMLESRVEPDEVTLASVISACASL--SAIKVGQEV 277

Query: 61  HGYARKLGLDTAHVMVGTSIVDMYAKCAQLGLARRVFDYLRMKNSVSWNTMLDGYTRNGE 120
           HG   K       +++  + VDMYAKC+++  AR +FD + ++N ++  +M+ GY     
Sbjct: 278 HGRVVKNDKLRNDIILSNAFVDMYAKCSRIKEARFIFDSMPIRNVIAETSMISGYAMAAS 337

Query: 121 IELAIDLFDEMPTRDAISWTALINGLLKQGYSEQALECFHQMQCSGIKPDYVSIIAVLAA 180
            + A  +F +M  R+ +SW ALI G  + G +E+AL  F  ++   + P + S   +L A
Sbjct: 338 TKAARLMFTKMAERNVVSWNALIAGYTQNGENEEALSLFCLLKRESVCPTHYSFANILKA 397

Query: 181 CADLGTLTLGLWVNRFVMQQEFK------DNIRISNSLIDMYSRCGCIGFARQVFERMSK 240
           CADL  L LG+  +  V++  FK      D+I + NSLIDMY +CGC+     VF +M +
Sbjct: 398 CADLAELHLGMQAHVHVLKHGFKFQSGEEDDIFVGNSLIDMYVKCGCVEEGYLVFRKMME 457

Query: 241 RTLVSWNSIIVGYAANGFADESLEFFDAMQKEGFKPDAVSYTGALTACSHAGLVNKGLEL 300
           R  VSWN++I+G+A NG+ +E+LE F  M + G KPD ++  G L+AC HAG V +G   
Sbjct: 458 RDCVSWNAMIIGFAQNGYGNEALELFREMLESGEKPDHITMIGVLSACGHAGFVEEGRHY 517

Query: 301 FDNMKRVHRIIPRIEHYGCIVDLYGRAGRLEDALDVIEKMPMKPNEVVLGSLLAACRTHG 360
           F +M R   + P  +HY C+VDL GRAG LE+A  +IE+MPM+P+ V+ GSLLAAC+ H 
Sbjct: 518 FSSMTRDFGVAPLRDHYTCMVDLLGRAGFLEEAKSMIEEMPMQPDSVIWGSLLAACKVHR 577

Query: 361 DVSLAERLMKHLSKLDPGGDSNYVLLSNIYAAIGKWEGANKVRRTMKARGVQKKPGFSSV 420
           +++L + + + L +++P     YVLLSN+YA +GKWE    VR++M+  GV K+PG S +
Sbjct: 578 NITLGKYVAEKLLEVEPSNSGPYVLLSNMYAELGKWEDVMNVRKSMRKEGVTKQPGCSWI 637

Query: 421 EIDGKVHEFVAGDKYHADADSIYSMLELLVHELK 449
           +I G  H F+  DK H     I+S+L++L+ E++
Sbjct: 638 KIQGHDHVFMVKDKSHPRKKQIHSLLDILIAEMR 669

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022142716.13.8e-27099.57pentatricopeptide repeat-containing protein At1g05750, chloroplastic [Momordica ... [more]
XP_038877228.12.7e-24790.30pentatricopeptide repeat-containing protein At1g05750, chloroplastic [Benincasa ... [more]
KAG7012718.14.8e-24187.07Pentatricopeptide repeat-containing protein, chloroplastic, partial [Cucurbita a... [more]
XP_023521260.14.8e-24187.28pentatricopeptide repeat-containing protein At1g05750, chloroplastic [Cucurbita ... [more]
XP_022945035.16.3e-24187.07pentatricopeptide repeat-containing protein At1g05750, chloroplastic [Cucurbita ... [more]
Match NameE-valueIdentityDescription
Q9MA503.9e-16962.72Pentatricopeptide repeat-containing protein At1g05750, chloroplastic OS=Arabidop... [more]
Q9LUJ26.6e-10037.91Pentatricopeptide repeat-containing protein At3g22690 OS=Arabidopsis thaliana OX... [more]
Q9LSB88.7e-10037.20Putative pentatricopeptide repeat-containing protein At3g15930 OS=Arabidopsis th... [more]
Q9SIT73.6e-9839.43Pentatricopeptide repeat-containing protein At2g13600 OS=Arabidopsis thaliana OX... [more]
O823801.4e-9736.77Pentatricopeptide repeat-containing protein At2g29760, chloroplastic OS=Arabidop... [more]
Match NameE-valueIdentityDescription
A0A6J1CN071.8e-27099.57pentatricopeptide repeat-containing protein At1g05750, chloroplastic OS=Momordic... [more]
A0A6J1FZR83.1e-24187.07pentatricopeptide repeat-containing protein At1g05750, chloroplastic OS=Cucurbit... [more]
A0A6J1HU312.0e-24087.07pentatricopeptide repeat-containing protein At1g05750, chloroplastic OS=Cucurbit... [more]
A0A5A7UJB61.1e-23385.99Pentatricopeptide repeat-containing protein OS=Cucumis melo var. makuwa OX=11946... [more]
A0A1S3C9561.1e-23385.99pentatricopeptide repeat-containing protein At1g05750, chloroplastic OS=Cucumis ... [more]
Match NameE-valueIdentityDescription
AT1G05750.12.8e-17062.72Tetratricopeptide repeat (TPR)-like superfamily protein [more]
AT3G22690.14.7e-10137.91CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF1685 (InterPro:IPR012... [more]
AT3G22690.24.7e-10137.91INVOLVED IN: photosystem II assembly, regulation of chlorophyll biosynthetic pro... [more]
AT3G15930.16.1e-10137.20Pentatricopeptide repeat (PPR) superfamily protein [more]
AT2G13600.12.6e-9939.43Pentatricopeptide repeat (PPR) superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3D1.25.40.10Tetratricopeptide repeat domaincoord: 63..190
e-value: 3.5E-30
score: 106.7
coord: 1..53
e-value: 4.6E-7
score: 31.3
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3D1.25.40.10Tetratricopeptide repeat domaincoord: 210..421
e-value: 3.4E-44
score: 153.5
IPR011990Tetratricopeptide-like helical domain superfamilySUPERFAMILY48452TPR-likecoord: 88..235
IPR002885Pentatricopeptide repeatPFAMPF01535PPRcoord: 106..133
e-value: 1.3E-8
score: 34.5
coord: 310..335
e-value: 0.0011
score: 19.1
coord: 210..235
e-value: 0.0013
score: 18.8
IPR002885Pentatricopeptide repeatTIGRFAMTIGR00756TIGR00756coord: 238..272
e-value: 2.5E-7
score: 28.5
coord: 273..300
e-value: 0.0027
score: 15.7
coord: 311..334
e-value: 3.7E-4
score: 18.5
coord: 106..134
e-value: 3.7E-8
score: 31.1
coord: 137..170
e-value: 3.5E-9
score: 34.3
IPR002885Pentatricopeptide repeatPFAMPF13041PPR_2coord: 135..182
e-value: 5.7E-10
score: 39.2
coord: 237..283
e-value: 8.1E-9
score: 35.6
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 236..270
score: 12.112294
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 135..169
score: 12.123256
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 1..34
score: 8.812943
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 104..134
score: 11.23539
NoneNo IPR availablePANTHERPTHR47926PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEINcoord: 1..457
NoneNo IPR availablePANTHERPTHR47926:SF65BNAANNG25350D PROTEINcoord: 1..457

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS002863.1MS002863.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005515 protein binding