MS002437 (gene) Bitter gourd (TR) v1

Overview
NameMS002437
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
Descriptioncell wall / vacuolar inhibitor of fructosidase 2-like
Locationscaffold318: 58956 .. 59456 (-)
RNA-Seq ExpressionMS002437
SyntenyMS002437
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ACTGACCCAATTGCAGTCTCGAATGATTTTGTTAAAGACACCTGTAAGAAACATGAAGAATTGTGCAAATTATGCATTAATGTGGTAGATTCAGATCCTCACCAAGACTTGAAATTAGACCTATTCGGGCTCTTAGCCATATTTATAAACCATTCTATTGCCAATGCCACCAACGATAATTTGTATCTTGTGGAGCAGTTCAAGAGACCAGGTTTAGACAATAGAACACAACAAATCTTCGATAGATGCATAGATGTGTACGGAAGGGGCATAGATGTTCTGACATCATCCTTAGAAACAGTGTTAAAGAAACATGATATAGAGTTGAGTATGACAATGACTGACGCGTTCGACTTGTTTGCGAGTTGTGAATATGAATATGAGTATACACCGGAACCTCCAACTTGGTTGTCTCGTTATCTTACCTTACGTTCACTTATGGAGCTCTCAATGGGGACTGCAAATCTCATAGAGTGTAAAAGGGTCGCCGCTTGTGTTCCA

mRNA sequence

ACTGACCCAATTGCAGTCTCGAATGATTTTGTTAAAGACACCTGTAAGAAACATGAAGAATTGTGCAAATTATGCATTAATGTGGTAGATTCAGATCCTCACCAAGACTTGAAATTAGACCTATTCGGGCTCTTAGCCATATTTATAAACCATTCTATTGCCAATGCCACCAACGATAATTTGTATCTTGTGGAGCAGTTCAAGAGACCAGGTTTAGACAATAGAACACAACAAATCTTCGATAGATGCATAGATGTGTACGGAAGGGGCATAGATGTTCTGACATCATCCTTAGAAACAGTGTTAAAGAAACATGATATAGAGTTGAGTATGACAATGACTGACGCGTTCGACTTGTTTGCGAGTTGTGAATATGAATATGAGTATACACCGGAACCTCCAACTTGGTTGTCTCGTTATCTTACCTTACGTTCACTTATGGAGCTCTCAATGGGGACTGCAAATCTCATAGAGTGTAAAAGGGTCGCCGCTTGTGTTCCA

Coding sequence (CDS)

ACTGACCCAATTGCAGTCTCGAATGATTTTGTTAAAGACACCTGTAAGAAACATGAAGAATTGTGCAAATTATGCATTAATGTGGTAGATTCAGATCCTCACCAAGACTTGAAATTAGACCTATTCGGGCTCTTAGCCATATTTATAAACCATTCTATTGCCAATGCCACCAACGATAATTTGTATCTTGTGGAGCAGTTCAAGAGACCAGGTTTAGACAATAGAACACAACAAATCTTCGATAGATGCATAGATGTGTACGGAAGGGGCATAGATGTTCTGACATCATCCTTAGAAACAGTGTTAAAGAAACATGATATAGAGTTGAGTATGACAATGACTGACGCGTTCGACTTGTTTGCGAGTTGTGAATATGAATATGAGTATACACCGGAACCTCCAACTTGGTTGTCTCGTTATCTTACCTTACGTTCACTTATGGAGCTCTCAATGGGGACTGCAAATCTCATAGAGTGTAAAAGGGTCGCCGCTTGTGTTCCA

Protein sequence

TDPIAVSNDFVKDTCKKHEELCKLCINVVDSDPHQDLKLDLFGLLAIFINHSIANATNDNLYLVEQFKRPGLDNRTQQIFDRCIDVYGRGIDVLTSSLETVLKKHDIELSMTMTDAFDLFASCEYEYEYTPEPPTWLSRYLTLRSLMELSMGTANLIECKRVAACVP
Homology
BLAST of MS002437 vs. NCBI nr
Match: XP_022137307.1 (uncharacterized protein LOC111008804 [Momordica charantia])

HSP 1 Score: 204.9 bits (520), Expect = 5.4e-49
Identity = 96/167 (57.49%), Postives = 129/167 (77.25%), Query Frame = 0

Query: 2   DPIAVSNDFVKDTCKK-HEELCKLCINVVDSDPHQDLKLDLFGLLAIFINHSIANATNDN 61
           + ++V+N FV +TC K  EE  K+CI+V+DSDPHQDLKL+L GLLAIFINHSI NAT+++
Sbjct: 5   EKLSVANSFVNNTCNKFEEEFIKVCIDVIDSDPHQDLKLNLSGLLAIFINHSIDNATDNH 64

Query: 62  LYLVEQFKRPGLDNRTQQIFDRCIDVYGRGIDVLTSSLETVLKKHDIELSMTMTDAFDLF 121
           LYL+EQ KR  LD   +  F+ C++ Y RG+D+L SSLE +LK    +L + ++DAFD  
Sbjct: 65  LYLMEQLKRSDLDKDIRYTFNVCVEQYERGLDILKSSLEALLKNDKDDLGLILSDAFDKI 124

Query: 122 ASCEYEYEYTPEPPTWLSRYLTLRSLMELSMGTANLIECKRVAACVP 168
            +CE ++E TPEPP+WL+ YL LR L+ LSMGTANLI+CK+VAAC+P
Sbjct: 125 VACEDDFESTPEPPSWLAHYLPLRKLLALSMGTANLIQCKKVAACIP 171

BLAST of MS002437 vs. NCBI nr
Match: KAG6572152.1 (Expansin-like A2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 124.8 bits (312), Expect = 7.1e-25
Identity = 60/166 (36.14%), Postives = 100/166 (60.24%), Query Frame = 0

Query: 5   AVSNDFVKDTCKK--HEELCKLCINVVDSDPHQDLKLDLFGLLAIFINHSIANATNDNLY 64
           + +N+F  DTC +   E+L KLCI  VDS+P +DLK +L G+L IF+NH+I+N  ++ ++
Sbjct: 150 STNNNFAHDTCNRFQFEQLYKLCIPEVDSEPREDLKSNLTGILIIFVNHTISNFKDNVMF 209

Query: 65  LVEQFKRPGLDNRTQQIFDRCIDVYGRGIDVLTSSLETVLKKHDIEL-SMTMTDAFDLFA 124
           L  + K   L+ +T++++D C++ + RG   L  S+  +L +   ++  +          
Sbjct: 210 LQREIKSGKLNGKTKEMYDTCLENFERGTTDLQQSMHVLLTETGKDIYGLPALSVASYVT 269

Query: 125 SCEYEYEYTPEPPTWLSRYLTLRSLMELSMGTANLIECKRVAACVP 168
            C  ++E  P+PP W SRY T   L+EL +GT NL++C R  AC+P
Sbjct: 270 ECVDDFEGVPKPPEWQSRYNTSNKLLELILGTCNLMKCNRELACIP 315

BLAST of MS002437 vs. NCBI nr
Match: KAG6599042.1 (hypothetical protein SDJN03_08820, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 124.0 bits (310), Expect = 1.2e-24
Identity = 67/162 (41.36%), Postives = 96/162 (59.26%), Query Frame = 0

Query: 6   VSNDFVKDTCKKHEELCKLCINVVDSDPHQDLKLDLFGLLAIFINHSIANATNDNLYLVE 65
           V+NDFV DTC K EEL  LC +V+ SDP  DLK +L  LL IFIN ++    +D+ YL  
Sbjct: 29  VANDFVHDTCNKTEELKDLCNSVIGSDPRDDLKTNLTSLLTIFINQTLRVVVDDHSYLYN 88

Query: 66  QFKRPGLDNRTQQIFDRCIDVYGRGIDVLTSSLET-VLKKHDIELSMTMTDAFDLFASCE 125
           Q     LDNRT ++F  C+++Y    D L + L+  +LK  + +L+M +    +    CE
Sbjct: 89  QTLNGELDNRTLEVFKSCMNLYQMSKDSLQALLDVELLKNKNADLNMDLAQVDNFLDLCE 148

Query: 126 YEYE-YTPEPPTWLSRYLTLRSLMELSMGTANLIECKRVAAC 166
            ++E +TPE  TW SRY  + SL+ LS+   NLI+C  +  C
Sbjct: 149 VDFEGFTPESLTWKSRYDYVSSLLILSLRMTNLIKCNHIHYC 190

BLAST of MS002437 vs. NCBI nr
Match: KAG6585821.1 (hypothetical protein SDJN03_18554, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 121.3 bits (303), Expect = 7.8e-24
Identity = 68/167 (40.72%), Postives = 99/167 (59.28%), Query Frame = 0

Query: 2   DP-IAVSNDFVKDTCKKHEELCKLCINVVDSDPHQDLKLDLFGLLAIFINHSIANATNDN 61
           DP +   +DF + TC +  EL  LCI+V++S+P +DLK +L G LAIFIN ++    +D+
Sbjct: 22  DPEMKAGSDFTRATCDRTSELKDLCISVINSNPCEDLKSNLTGFLAIFINETLHVVIDDH 81

Query: 62  LYLVEQFKRPGLDNRTQQIFDRCIDVYGRGIDVLTSSLE-TVLKKHDIELSMTMTDAFDL 121
            YL+ Q     LDNRT Q+F  C   Y    D L   LE  +LK+H+ +L+M +    + 
Sbjct: 82  SYLLNQTLNGQLDNRTLQVFRSCASQYQMSKDSLRVLLEDELLKQHNADLNMDLGQIDNY 141

Query: 122 FASCEYEYE-YTPEPPTWLSRYLTLRSLMELSMGTANLIECKRVAAC 166
            A CE E+E +  EP  W +RY  + SL+ LS+ T NLI+C R+  C
Sbjct: 142 LALCEMEFEGFIEEPSAWKTRYDYVSSLLILSLQTTNLIKCNRIHVC 188

BLAST of MS002437 vs. NCBI nr
Match: KAG6599036.1 (hypothetical protein SDJN03_08814, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 120.6 bits (301), Expect = 1.3e-23
Identity = 65/160 (40.62%), Postives = 95/160 (59.38%), Query Frame = 0

Query: 8   NDFVKDTCKKHEELCKLCINVVDSDPHQDLKLDLFGLLAIFINHSIANATNDNLYLVEQF 67
           NDF++DTC + E+   LCI+V+DSDP +DLKL+    L IFIN ++    +D+ YL+ Q 
Sbjct: 5   NDFIQDTCNRTEDFKDLCISVIDSDPREDLKLNSMDFLVIFINRTLQVIIDDHSYLLNQT 64

Query: 68  KRPGLDNRTQQIFDRCIDVYGRGIDVLTSSL-ETVLKKHDIELSMTMTDAFDLFASCEYE 127
               LDNRT+ IF  C+ +Y    D L   L E +LK+ + +L+M +         C+ +
Sbjct: 65  LNEELDNRTRHIFMGCMTLYQMSKDSLRVLLNEELLKQPNADLNMKLQQIEGYLDLCQND 124

Query: 128 YE-YTPEPPTWLSRYLTLRSLMELSMGTANLIECKRVAAC 166
           +E +TP P TW SRY  + SL+ LS    NLI+C  + AC
Sbjct: 125 FEGFTPTPSTWTSRYDYVISLLILSFRMMNLIKCNYIRAC 164

BLAST of MS002437 vs. ExPASy TrEMBL
Match: A0A6J1C7W5 (uncharacterized protein LOC111008804 OS=Momordica charantia OX=3673 GN=LOC111008804 PE=4 SV=1)

HSP 1 Score: 204.9 bits (520), Expect = 2.6e-49
Identity = 96/167 (57.49%), Postives = 129/167 (77.25%), Query Frame = 0

Query: 2   DPIAVSNDFVKDTCKK-HEELCKLCINVVDSDPHQDLKLDLFGLLAIFINHSIANATNDN 61
           + ++V+N FV +TC K  EE  K+CI+V+DSDPHQDLKL+L GLLAIFINHSI NAT+++
Sbjct: 5   EKLSVANSFVNNTCNKFEEEFIKVCIDVIDSDPHQDLKLNLSGLLAIFINHSIDNATDNH 64

Query: 62  LYLVEQFKRPGLDNRTQQIFDRCIDVYGRGIDVLTSSLETVLKKHDIELSMTMTDAFDLF 121
           LYL+EQ KR  LD   +  F+ C++ Y RG+D+L SSLE +LK    +L + ++DAFD  
Sbjct: 65  LYLMEQLKRSDLDKDIRYTFNVCVEQYERGLDILKSSLEALLKNDKDDLGLILSDAFDKI 124

Query: 122 ASCEYEYEYTPEPPTWLSRYLTLRSLMELSMGTANLIECKRVAACVP 168
            +CE ++E TPEPP+WL+ YL LR L+ LSMGTANLI+CK+VAAC+P
Sbjct: 125 VACEDDFESTPEPPSWLAHYLPLRKLLALSMGTANLIQCKKVAACIP 171

BLAST of MS002437 vs. ExPASy TrEMBL
Match: A0A5D3BJB5 (Putative invertase inhibitor OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G004170 PE=4 SV=1)

HSP 1 Score: 108.6 bits (270), Expect = 2.5e-20
Identity = 59/162 (36.42%), Postives = 91/162 (56.17%), Query Frame = 0

Query: 8   NDFVKDTCKKHEELCKLCINVVDSDPHQDLKLDLFGLLAIFINHSIANATNDNLYLVEQF 67
           NDFV+ TC +  EL  LC++ + S+P  DLK +L GLL IF+N SI+N  ND  +L ++ 
Sbjct: 29  NDFVRHTCNRTHELKNLCLSQISSEPRNDLKSNLTGLLMIFVNQSISNFKNDISFLEKEI 88

Query: 68  KRPGLDNRTQQIFDRCIDVYGRGIDVLTSSLETVLKK--HDIELSMTMTDAFDLFASCEY 127
               +   T+ + + C+  +  G   L  ++E +  K  +D E  + + +  +L   C  
Sbjct: 89  NSNKISRDTKDMLEDCLQNFQIGSVNLRETMEILQTKTGYDAE-QLPVGNVGNLAVECFD 148

Query: 128 EYEYTPEPPTWLSRYLTLRSLMELSMGTANLIECKRVAACVP 168
           ++E TP PP W SRY    +L++LSM   NLI C R  AC+P
Sbjct: 149 DFEGTPVPPEWQSRYNASFALLKLSMAMINLITCNRDYACIP 189

BLAST of MS002437 vs. ExPASy TrEMBL
Match: A0A1U7YVD9 (cell wall / vacuolar inhibitor of fructosidase 2-like OS=Nelumbo nucifera OX=4432 GN=LOC104587371 PE=4 SV=1)

HSP 1 Score: 58.2 bits (139), Expect = 3.9e-05
Identity = 36/113 (31.86%), Postives = 56/113 (49.56%), Query Frame = 0

Query: 25  CINVVDSDPHQDLKLDLFGLLAIFINHSIANATNDNLYLVEQFKRPGLDNRTQQIFDRCI 84
           CI++++SDPH +LK DL G   I +   ++NATND  Y+ E  K    DN T      C 
Sbjct: 52  CISLLESDPHSELKSDLNGFARIIMEVGLSNATNDYDYIGELVKNT-TDNATLSFLHTCS 111

Query: 85  DVYGRGIDVLTSSLETV--LKKHDIELSMTMTDAFDLFASCEYEYEYTPEPPT 136
            +Y + I    SS   +  +K + ++L  T+  +      C  E  + P+P T
Sbjct: 112 SLYEKTIAGYNSSFNMLSDIKGNYVQLYQTLDSSIQNAQDC--EDAFAPQPNT 161

BLAST of MS002437 vs. TAIR 10
Match: AT5G46930.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 45.4 bits (106), Expect = 5.1e-05
Identity = 32/135 (23.70%), Postives = 62/135 (45.93%), Query Frame = 0

Query: 5   AVSNDFVKDTCKK-----HEELCKLCINVVDSDPHQDLKLDLFGLLAIFINHSIANATN- 64
           A +   ++D+CKK      +     C+  ++ +P       L GL+ +   ++++  T+ 
Sbjct: 18  ATAQSLIRDSCKKATTKDPKLKYDFCVKSLEENPQSKTAKSLEGLVFVSTKNAVSKTTSL 77

Query: 65  ----DNLYLVEQF--KRPGLDNRTQQIFDRCIDVYGRGIDVLTSSLETVLKKHDIELSMT 124
               D +   +++  +RP LD         C+++Y   ID L  SL+TV  +     +M 
Sbjct: 78  KGMVDKILKEDKYEVERPLLD---------CLELYTEAIDSLNQSLDTVKSRDYKTATML 137

Query: 125 MTDAFDLFASCEYEY 128
           M+ A D   SCE ++
Sbjct: 138 MSAAMDAPGSCETKF 143

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022137307.15.4e-4957.49uncharacterized protein LOC111008804 [Momordica charantia][more]
KAG6572152.17.1e-2536.14Expansin-like A2, partial [Cucurbita argyrosperma subsp. sororia][more]
KAG6599042.11.2e-2441.36hypothetical protein SDJN03_08820, partial [Cucurbita argyrosperma subsp. sorori... [more]
KAG6585821.17.8e-2440.72hypothetical protein SDJN03_18554, partial [Cucurbita argyrosperma subsp. sorori... [more]
KAG6599036.11.3e-2340.63hypothetical protein SDJN03_08814, partial [Cucurbita argyrosperma subsp. sorori... [more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A6J1C7W52.6e-4957.49uncharacterized protein LOC111008804 OS=Momordica charantia OX=3673 GN=LOC111008... [more]
A0A5D3BJB52.5e-2036.42Putative invertase inhibitor OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca... [more]
A0A1U7YVD93.9e-0531.86cell wall / vacuolar inhibitor of fructosidase 2-like OS=Nelumbo nucifera OX=443... [more]
Match NameE-valueIdentityDescription
AT5G46930.15.1e-0523.70Plant invertase/pectin methylesterase inhibitor superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilyGENE3D1.20.140.40Invertase/pectin methylesterase inhibitor family proteincoord: 6..154
e-value: 2.0E-13
score: 52.6
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilySUPERFAMILY101148Plant invertase/pectin methylesterase inhibitorcoord: 8..146
IPR006501Pectinesterase inhibitor domainTIGRFAMTIGR01614TIGR01614coord: 6..131
e-value: 2.2E-15
score: 54.4
IPR006501Pectinesterase inhibitor domainPFAMPF04043PMEIcoord: 9..124
e-value: 3.2E-8
score: 34.0

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS002437.1MS002437.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0004857 enzyme inhibitor activity